CN104663861A - Method for extraction of biological fresh-keeping agent from honeysuckle flower and use of biological fresh-keeping agent in mulberry fresh-keeping - Google Patents
Method for extraction of biological fresh-keeping agent from honeysuckle flower and use of biological fresh-keeping agent in mulberry fresh-keeping Download PDFInfo
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- CN104663861A CN104663861A CN201310613223.0A CN201310613223A CN104663861A CN 104663861 A CN104663861 A CN 104663861A CN 201310613223 A CN201310613223 A CN 201310613223A CN 104663861 A CN104663861 A CN 104663861A
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- Medicines Containing Plant Substances (AREA)
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Abstract
The invention relates to a method for extraction of a biological fresh-keeping agent from honeysuckle flower. The method comprises the following steps of 1, cleaning and selecting honeysuckle flower so that leaves, branches and other foreign materials are removed, 2, crushing the honeysuckle flower to obtain powder of 100-200 meshes, 3, adding the honeysuckle flower powder into an ethanol solution according to a material-liquid ratio of 1: 5-10, carrying out shaking mixing, transferring the mixture into a microwave extraction apparatus and carrying out microwave extraction at a temperature of 65-75 DEG C at microwave power of 500-800w for 5-10min, and 4, filtering the extract to obtain filtrate, and storing the filtrate at a temperature of 2-4 DEG C. The invention also provides a use of the biological fresh-keeping agent in mulberry fresh-keeping. The method extracts the biological fresh-keeping agent from honeysuckle flower as a raw material and the biological fresh-keeping agent is used for mulberry fresh-keeping and is safe. The biological fresh-keeping agent is nontoxic and nuisanceless, can replace a chemical bactericide and an antiseptic for mulberry fresh-keeping, and prevents chemical bactericide-caused environmental pollution and food safety problems.
Description
Technical field
The present invention relates to the field of preserving fruit and vegetable utilizing, the bio-preservative extracted from honeysuckle specifically and the application in mulberries are fresh-keeping thereof.
Background technology
Honeysuckle, has another name called two flowers, and be dry flower or the flower just opened of Caprifoliaceae woodbine honeysuckle, in China, most area all has production, is China's traditional Chinese medicine material, taste is sweet, cold in nature, clearing heat and detoxicating, wind-heat dissipating.Modern chemical analysis and pharmacological evaluation prove, honeysuckle have broad-spectrum antiseptic, antiviral, antitumor, strengthen immunity and antipyretic and anti-inflammatory, cholagogic, protect the liver, lipopenicillinase, antifertility, hemostasis, the pharmacological action such as antiulcer.It is reported, the whole nation has the bud of more than 20 Plants to be used as medicine when honeysuckle, is all Caprifoliaceae woodbine.Owing to having special drug effect, therefore become the study hotspot of Chinese herbal medicine in recent years.Containing cyanidenon in honeysuckle, isochlorogenic acid, the multiple compounds such as chlorogenic acid and flavones, tradition thinks that chlorogenic acid is the principle active component of honeysuckle, but show according to modern medicine study, flavone compound wherein has reduction MCO, make coronary artery, cerebrovascular flow increases, the anti-rhythm of the heart, softening blood vessel, hypoglycemic, the effects such as blood fat, simultaneously it or a kind of natural antioxidant, there is Superoxide anion free radical in removing human body anti-ageing, increase the physiological activity of immunity of organisms, therefore its biologically active becomes the focus of people's research day by day.
Mulberries, be the ripening fruits of Moraceae deciduous tree mulberry tree, mulberries are again sorosis, mulberry mulberries.Traditional Chinese medicine thinks that mulberry fruit taste is sour, cold nature, have improve body immunity, enriching yin of enriching blood, promote the production of body fluid to quench thirst, the effect such as dry is dispelled in ease constipation, cure mainly deficiency of YIN-blood and cause have a dizzy spell, tinnitus palpitaition, irritated insomnia, soreness and weakness of waist and knees, poliosis, quench one's thirst only do, dry and hard excrement, nourishing yin and supplementing blood, improving eyesight are calmed the nerves, sharp joint, curing rheumatism, the effect such as to relieve the effect of alcohol.
Mulberries belong to berries fruit, delicious flavour, rich in nutritive value, but the respiratory intensity of mulberries is large, and protective tissue is poor, and preservation and freshness is more difficult, and preservation term and shelf life are all shorter, can bring great loss like this for orchard worker and consumer.And the preservation method of berries fruit conventional at present mainly adopts chemical bactericide, and a large amount of uses of chemical bactericide, pathogenic microorganism can be caused to produce drug resistance, and environmental pollution; In addition, because mulberries cleaning is more difficult, be difficult to thoroughly wash away chemical bactericide, after edible, health hidden danger can be caused.
Summary of the invention
According to above-mentioned weak point, the object of this invention is to provide a kind of with the honeysuckle extraction method of bio-preservative and the application in mulberries are fresh-keeping thereof.
For achieving the above object, technical scheme of the present invention is: a kind of method extracting bio-preservative from honeysuckle, comprises the steps, 1, honeysuckle is cleaned, and removing leaf, branch and other foreign material; 2, pulverizer is adopted by honeysuckle to be crushed to 100-200 order; 3, be that the ratio of 1:5-10 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 65-75 DEG C, and the working time is 5-10min, and microwave power is 500-800w; 4, by extracting liquid filtering, filtrate is obtained, and in 2-4 DEG C of storage.
Preferably: described ethanol solution concentration is 50-60%.
Preferably: add rotor in described leaching process and stir.
The present invention also provides a kind of application of bio-preservative in mulberries are fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the mulberries newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.
Preferably: described mulberries holding conditions is relative humidity 90-95%, temperature is 2 ± 0.5 DEG C.
Beneficial effect of the present invention is: the present invention is that raw material extraction bio-preservative carries out fresh-keeping to mulberries with honeysuckle, safety.Nontoxic, nuisanceless, fresh-keeping for mulberries of chemical bactericide, anticorrisive agent can be replaced, avoid the problem such as pollution, food security of chemical bactericide to environment.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention will be further described.
Embodiment 1
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted by honeysuckle to be crushed to 100 orders; 2, be that the ratio of 1:5 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 65 DEG C, and the working time is 5min, and microwave power is 500w; 3, by extracting liquid filtering, filtrate is obtained, and in 2 DEG C of storages.Wherein, ethanol solution concentration is 50%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in mulberries are fresh-keeping of extracting according to method described in claim 1, immerses 3min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the mulberries newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Mulberries holding conditions is relative humidity 90%, and temperature is 1.5 DEG C.
Embodiment 2
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted by honeysuckle to be crushed to 200 orders; 3, be that the ratio of 1:10 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 75 DEG C, and the working time is 10min, and microwave power is 800w; 3, by extracting liquid filtering, filtrate is obtained, and in 4 DEG C of storages.Wherein, ethanol solution concentration is 60%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in mulberries are fresh-keeping of extracting according to method described in claim 1, immerses 3min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the mulberries newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Mulberries holding conditions is relative humidity 95%, and temperature is 2.5 DEG C.
Embodiment 3
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted to be crushed to 150 orders honeysuckle; 3, be that the ratio of 1:7 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 70 DEG C, and the working time is 7min, and microwave power is 600w; 4, by extracting liquid filtering, filtrate is obtained, and in 3 DEG C of storages.Wherein, ethanol solution concentration is 55%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in mulberries are fresh-keeping of extracting according to method described in claim 1, immerses 3min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the mulberries newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Mulberries holding conditions is relative humidity 90%, and temperature is 2 DEG C.
Embodiment 4
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted by honeysuckle to be crushed to 190 orders; 3, be that the ratio of 1:8 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 72 DEG C, and the working time is 9min, and microwave power is 580w; 4, by extracting liquid filtering, filtrate is obtained, and in 2 DEG C of storages.Wherein, ethanol solution concentration is 55%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in mulberries are fresh-keeping of extracting according to method described in claim 1, immerses 3min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the mulberries newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Mulberries holding conditions is relative humidity 90%, and temperature is 2 DEG C.
Claims (5)
1. from honeysuckle, extract a method for bio-preservative, it is characterized in that: comprise the steps,
(1) honeysuckle is cleaned, removing leaf, branch and other foreign material;
(2) pulverizer is adopted by honeysuckle to be crushed to 100-200 order;
(3) be that the ratio of 1:5-10 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 65-75 DEG C, and the working time is 5-10min, and microwave power is 500-800w;
(4) by extracting liquid filtering, filtrate is obtained, and in 2-4 DEG C of storage.
2. according to the method extracting bio-preservative from honeysuckle according to claim 1, it is characterized in that: described ethanol solution concentration is 50-60%.
3. according to the method extracting bio-preservative from honeysuckle according to claim 1, it is characterized in that: add rotor in described leaching process and stir.
4. the application of bio-preservative in mulberries are fresh-keeping of extracting according to method described in claim 1, it is characterized in that: after the mulberries newly plucked being rejected insect pest and mechanical damage fruit, immerse 3min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.
5. according to the application of bio-preservative according to claim 4 in mulberries are fresh-keeping, it is characterized in that: described mulberries holding conditions is relative humidity 90-95%, temperature is 2 ± 0.5 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310613223.0A CN104663861A (en) | 2013-11-26 | 2013-11-26 | Method for extraction of biological fresh-keeping agent from honeysuckle flower and use of biological fresh-keeping agent in mulberry fresh-keeping |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310613223.0A CN104663861A (en) | 2013-11-26 | 2013-11-26 | Method for extraction of biological fresh-keeping agent from honeysuckle flower and use of biological fresh-keeping agent in mulberry fresh-keeping |
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| CN104663861A true CN104663861A (en) | 2015-06-03 |
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| CN201310613223.0A Pending CN104663861A (en) | 2013-11-26 | 2013-11-26 | Method for extraction of biological fresh-keeping agent from honeysuckle flower and use of biological fresh-keeping agent in mulberry fresh-keeping |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904840A (en) * | 2015-06-24 | 2015-09-16 | 南京大地冷冻食品有限公司 | Cucumber and honeysuckle flower coating preservation solution |
| CN104996554A (en) * | 2015-06-24 | 2015-10-28 | 南京大地冷冻食品有限公司 | Application of cucumber and honeysuckle coating preservation liquid in freshness preservation of fruits, vegetables and chilled meat |
| CN110973531A (en) * | 2019-12-04 | 2020-04-10 | 齐鲁工业大学 | Method for preparing high-nutrition white mulberry instant powder by spray freeze drying |
-
2013
- 2013-11-26 CN CN201310613223.0A patent/CN104663861A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904840A (en) * | 2015-06-24 | 2015-09-16 | 南京大地冷冻食品有限公司 | Cucumber and honeysuckle flower coating preservation solution |
| CN104996554A (en) * | 2015-06-24 | 2015-10-28 | 南京大地冷冻食品有限公司 | Application of cucumber and honeysuckle coating preservation liquid in freshness preservation of fruits, vegetables and chilled meat |
| CN110973531A (en) * | 2019-12-04 | 2020-04-10 | 齐鲁工业大学 | Method for preparing high-nutrition white mulberry instant powder by spray freeze drying |
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Application publication date: 20150603 |