CN104642726A - Microbial detoxication method for cotton seed meal - Google Patents
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- 238000000855 fermentation Methods 0.000 claims abstract description 37
- 230000004151 fermentation Effects 0.000 claims abstract description 37
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- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 13
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims abstract description 10
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 8
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- 239000004475 Arginine Substances 0.000 description 2
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- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
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- 239000004472 Lysine Substances 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
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- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a microbial detoxication method for cotton seed meal. The method comprises the following steps: grinding cotton seed meal, adding wheat bran, corn flour, glucose, urea, ferrous sulfate heptahydrate, sodium chloride and manganese sulfate into the ground cotton seed meal, uniformly mixing, and regulating the moisture content, and thus preparing a cottonseed meal detoxicated fermentation medium; adding 4-8 percent of a detoxicated microbial fermentation agent consisting of brewer's yeasts, bacillus subtilis, candida utilis lactobacillus acidophilus and Aspergillus oryzae, performing low-temperature fermentation and detoxicated fermentation for 4-6 days, and drying, thereby obtaining the product. The microbial detoxication method disclosed by the invention is simple in process, low in energy consumption and low in investment, the three wastes are avoided, large-scale production is easily realized, the content of free gossypol can be reduced by over 85 percent, certain organic acids can be produced, the pH value of the matrix is reduced, the taste of the cottonseed meal is improved, the nutritive value of the cottonseed meal is obviously improved, and the content of crude proteins is improved by over 15 percent compared with that before fermentation.
Description
Technical field
The invention belongs to field of feed processing, particularly a kind of microbiological detoxifying method of cotton cake dregs.
Background technology
China is the first in the world Chan Mian big country, the whole nation is annual approximately produces 710 ~ 750 tons of cottonseeds, cotton cake dregs is the accessory substance after cottonseed shelling is deoiled, its output is only second to soya-bean cake, cotton cake dregs is the byproduct after cottonseed extracts oil, utilize it as feed, not only there is the characteristic of protein feed but also there is the characteristic of energy feed.Cotton cake dregs crude protein content is higher, reaches more than 34%, and Cottonseed meal thick protein can reach 41% ~ 60%.From the amino acid content of cotton cake dregs, possess 8 kinds of essential amino acids, lysine is up to 6%, and the content of cystine and tryptophan is relatively abundant; Arginine content, far above dregs of beans, is 2 times of dregs of rapeseed cake, is only second to peanut dregs, and arginine reaches more than 2.7 with lysine ratio.In addition, also abundant vitamin E and B family vitamin etc. are contained in cotton cake dregs.But because cotton cake dregs contains toxicant gossypol, and make it utilize to be restricted, the high cotton cake dregs of most of free gossypol content, only as Fertilizer application, wastes valuable protein resource.In order to strengthen the consumption of cotton cake dregs in feed, just detoxification treatment must be carried out to cotton cake dregs.
Traditional poison-removing method comprises organic extraction, ferrous sulfate process, calcium hydroxide process etc., wherein, organic solvent leach extraction method causes organic solvent residual, has potential harm to livestock and poultry, ferrous sulfate process can reduce feed quality, and calcium hydroxide process detoxification efficiency is low.There is the reduction of nutriment in physics detoxicity method and bound gossoypol still can decomposite free gossypol under the effect of enteric microorganism, and in During Detoxification, energy consumption is high, feed palatability is poor, is difficult to suitability for industrialized production.
The method of fermentable is a kind of method with development prospect, fermentable not only can remove the gossypol in cotton cake dregs, but also the protein content of cotton cake dregs can be provided, many kinds of enzymes are retained in fermentation substrate, vitamin, amino acid, and somatomedin, therefore microbial fermentation technology is utilized, development of new feed resource, production protein feeds and new additive agent more and more come into one's own, after particularly entering 21 century, utilize the forage protein of micro-organisms, enzyme preparation, amino acid, vitamin, antibiotic and probio, the use of the feed products such as microorganism formulation makes fermentation engineering obtain in feed industry to apply widely.Under the effect of the efficient-decomposition factor, crude fibre etc. can not be changed into the low molecular compound that can doubly absorb by the polymeric carbohydrate that livestock and poultry absorb; In addition multiple beneficial flora can absorb organic nitrogen, the inorganic nitrogen that can not be absorbed by livestock and poultry or utilize, and makes it be converted into the higher bacterium protein of nutritional labeling; And profitable strain can increase immunity of livestock, reduce the generation of disease.Although adopt biological detoxication method to achieve good effect, also there is the shortcomings such as the more complicated and microorganism bad adaptability of detoxification efficiency instability, technique.
Summary of the invention
The technical problem to be solved in the present invention overcomes existing defect, provides a kind of microbiological detoxifying method of cotton cake dregs, and this technique comprises following step:
1) cotton cake dregs is ground into the fragment of diameter at 0.6 ~ 1.2mm;
2) cotton cake dregs fragment 80 ~ 85 parts, 8 ~ 12 parts, wheat bran, corn flour 6 ~ 10 parts is got, glucose 1.0 ~ 2.5 parts, 0.6 ~ 1.0 part, urea, ferrous sulfate heptahydrate 0.1 ~ 0.15 part, 1 ~ 1.5 part, sodium chloride, manganese sulfate 0.06 ~ 0.1 part mixing, regulate wet weight to be 50% ~ 55%, make cotton cake toxicity removal fermentation medium;
3) in cotton cake toxicity removal fermentation medium, add detoxification microbe leaven, the consumption of detoxification microbe leaven is 4 ~ 8% of cotton cake dregs quality;
Wherein detoxification microbe leaven is saccharomyces cerevisiae 70 ~ 85 parts, bacillus subtilis 25 ~ 35 parts, candida utili lactobacillus acidophilus 20 ~ 30 parts, aspergillus oryzae 30 ~ 35 parts;
4) cold fermentation
25 ~ 37 DEG C, under stir speed (S.S.) is the condition of 40 ~ 60r/min, cold fermentation detoxification fermentation 4 ~ 6 days, namely obtains detoxification cotton dregs after drying.
Further, described step 3) in detoxification microbe leaven detoxification microbe leaven be saccharomyces cerevisiae 78 parts, bacillus subtilis 30 parts, candida utili lactobacillus acidophilus 25 parts, aspergillus oryzae 34 parts.
Further, described step 2) in cotton cake toxicity removal fermentation medium be cotton cake dregs fragment 82 parts, 10 parts, wheat bran, corn flour 8 parts, glucose 1.5 parts, 0.7 part, urea, ferrous sulfate heptahydrate 0.1 part, 1.2 parts, sodium chloride, manganese sulfate 0.08 mixes, and regulates wet weight to be 52%.
Further, described step 4) in cold fermentation condition be 32 DEG C, under stir speed (S.S.) is the condition of 55r/min, cold fermentation detoxification is fermented 5 days.
Compared with prior art, advantage of the present invention and beneficial effect are:
Microbiological detoxifying method of the present invention; technique is simple, energy consumption is low, without the three wastes, invest little, be easy to large-scale production; after the process that undergoes microbial fermentation; free gossypol can decline more than 85% relative to the content in the raw cotton dregs of rice; microbial growth, breeding metabolism etc. are movable creates certain organic acid as lactic acid, acetic acid, propionic acid, butyric acid etc.; reduce the pH value of matrix; improve the mouthfeel of cotton dregs of knowing clearly; the nutritive value of cotton dregs is improved significantly, and improves more than 15% before crude protein content comparatively ferments.
Detailed description of the invention
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein is only for instruction and explanation of the present invention, is not intended to limit the present invention.
Embodiment 1
A microbiological detoxifying method for cotton cake dregs, this technique comprises following step:
1) cotton cake dregs is ground into the fragment of diameter at 0.6mm;
2) get cotton cake dregs fragment 80 parts, 8 parts, wheat bran, corn flour 6 parts, glucose 1.0 parts, 0.6 part, urea, ferrous sulfate heptahydrate 0.1 part, 1 part, sodium chloride, manganese sulfate 0.06 part mixing, regulate wet weight to be 50%, make cotton cake toxicity removal fermentation medium;
3) in cotton cake toxicity removal fermentation medium, add detoxification microbe leaven, the consumption of detoxification microbe leaven is 4% of cotton cake dregs quality;
Wherein detoxification microbe leaven is saccharomyces cerevisiae 70 parts, bacillus subtilis 25 parts, candida utili lactobacillus acidophilus 20 parts, aspergillus oryzae 30 parts;
4) cold fermentation
25 DEG C, under stir speed (S.S.) is the condition of 40r/min, cold fermentation detoxification is fermented 6 days, namely obtains detoxification cotton dregs after drying.
Embodiment 2
A microbiological detoxifying method for cotton cake dregs, this technique comprises following step:
1) cotton cake dregs is ground into the fragment of diameter at 1.2mm;
2) cotton cake dregs fragment 85 parts, 12 parts, wheat bran, corn flour 10 parts is got, glucose 2.5 parts, 1.0 parts, urea, ferrous sulfate heptahydrate 0.15 part, 1.5 parts, sodium chloride, manganese sulfate 0.1 part mixing, regulates wet weight to be 55%, makes cotton cake toxicity removal fermentation medium;
3) in cotton cake toxicity removal fermentation medium, add detoxification microbe leaven, the consumption of detoxification microbe leaven is 8% of cotton cake dregs quality;
Wherein detoxification microbe leaven is saccharomyces cerevisiae 85 parts, bacillus subtilis 35 parts, candida utili lactobacillus acidophilus 30 parts, aspergillus oryzae 35 parts;
4) cold fermentation
37 DEG C, under stir speed (S.S.) is the condition of 60r/min, cold fermentation detoxification is fermented 4 days, namely obtains detoxification cotton dregs after drying.
Embodiment 3
A microbiological detoxifying method for cotton cake dregs, this technique comprises following step:
1) cotton cake dregs is ground into the fragment of diameter at 0.8mm;
2) cotton cake dregs fragment 82 parts, 10 parts, wheat bran, corn flour 8 parts is got, glucose 1.5 parts, 0.7 part, urea, ferrous sulfate heptahydrate 0.1 part, 1.2 parts, sodium chloride, manganese sulfate 0.08 part mixing, regulates wet weight to be 52%, makes cotton cake toxicity removal fermentation medium;
3) in cotton cake toxicity removal fermentation medium, add detoxification microbe leaven, the consumption of detoxification microbe leaven is 6% of cotton cake dregs quality;
Wherein detoxification microbe leaven is saccharomyces cerevisiae 78 parts, bacillus subtilis 30 parts, candida utili lactobacillus acidophilus 25 parts, aspergillus oryzae 34 parts;
4) cold fermentation
32 DEG C, under stir speed (S.S.) is the condition of 55r/min, cold fermentation detoxification is fermented 5 days, namely obtains detoxification cotton dregs after drying.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. a microbiological detoxifying method for cotton cake dregs, is characterized in that comprising the following steps:
1) cotton cake dregs is ground into the fragment of diameter at 0.6 ~ 1.2mm;
2) cotton cake dregs fragment 80 ~ 85 parts, 8 ~ 12 parts, wheat bran, corn flour 6 ~ 10 parts is got, glucose 1.0 ~ 2.5 parts, 0.6 ~ 1.0 part, urea, ferrous sulfate heptahydrate 0.1 ~ 0.15 part, 1 ~ 1.5 part, sodium chloride, manganese sulfate 0.06 ~ 0.1 part mixing, regulate wet weight to be 50% ~ 55%, make cotton cake toxicity removal fermentation medium;
3) in cotton cake toxicity removal fermentation medium, add detoxification microbe leaven, the consumption of detoxification microbe leaven is 4 ~ 8% of cotton cake dregs quality;
Wherein detoxification microbe leaven is saccharomyces cerevisiae 70 ~ 85 parts, bacillus subtilis 25 ~ 35 parts, candida utili lactobacillus acidophilus 20 ~ 30 parts, aspergillus oryzae 30 ~ 35 parts;
4) cold fermentation
25 ~ 37 DEG C, under stir speed (S.S.) is the condition of 40 ~ 60r/min, cold fermentation detoxification fermentation 4 ~ 6 days, namely obtains detoxification cotton dregs after drying.
2. the microbiological detoxifying method of a kind of cotton cake dregs as claimed in claim 1, it is characterized in that, described step 3) in detoxification microbe leaven detoxification microbe leaven be saccharomyces cerevisiae 78 parts, bacillus subtilis 30 parts, candida utili lactobacillus acidophilus 25 parts, aspergillus oryzae 34 parts.
3. the microbiological detoxifying method of a kind of cotton cake dregs as claimed in claim 1, it is characterized in that, described step 2) in cotton cake toxicity removal fermentation medium be cotton cake dregs fragment 82 parts, 10 parts, wheat bran, corn flour 8 parts, glucose 1.5 parts, 0.7 part, urea, ferrous sulfate heptahydrate 0.1 part, 1.2 parts, sodium chloride, manganese sulfate 0.08 mixes, and regulates wet weight to be 52%.
4. the microbiological detoxifying method of a kind of cotton cake dregs as claimed in claim 1, is characterized in that, described step 4) in cold fermentation condition be 32 DEG C, under stir speed (S.S.) is the condition of 55r/min, cold fermentation detoxification is fermented 5 days.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938787A (en) * | 2015-07-04 | 2015-09-30 | 新疆泰昆集团股份有限公司 | Cottonseed meal compound microbial ecological agent suitable for aquaculture and preparation and application |
| CN105918642A (en) * | 2016-05-12 | 2016-09-07 | 巢湖市鑫宇良种农民专业合作社 | Method for preparing feed from cottonseed meal |
| CN105995029A (en) * | 2016-05-12 | 2016-10-12 | 巢湖市鑫宇良种农民专业合作社 | Cottonseed-meal detoxification method |
| CN112369499A (en) * | 2020-10-21 | 2021-02-19 | 珠海市德海生物科技有限公司 | Feed additive, sea bass feed containing high dephenolized cottonseed protein and preparation method of sea bass feed |
| CN112515038A (en) * | 2020-12-03 | 2021-03-19 | 赵春海 | Method for treating cottonseed meal by microbial solid fermentation |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104938787A (en) * | 2015-07-04 | 2015-09-30 | 新疆泰昆集团股份有限公司 | Cottonseed meal compound microbial ecological agent suitable for aquaculture and preparation and application |
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| CN112369499A (en) * | 2020-10-21 | 2021-02-19 | 珠海市德海生物科技有限公司 | Feed additive, sea bass feed containing high dephenolized cottonseed protein and preparation method of sea bass feed |
| CN112515038A (en) * | 2020-12-03 | 2021-03-19 | 赵春海 | Method for treating cottonseed meal by microbial solid fermentation |
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