CN104623554A - Powder for removing slough and promoting growth of tissue regeneration to improve incision healing of lower limbs - Google Patents

Abstract

The invention provides a kind of Qurot Shengji powder, the raw materials of which include myrrh, frankincense, catechu, calamine, calamine, elephant skin, light powder, musk, borneol, dried blood, calamine, yin Fragrant skin, buffalo horn, white pomegranate, astragalus, mountain white chrysanthemum, burdock seed, chamomile, white wine grass and turmeric. The exogenous recombinant bovine basic fibroblast growth factor of Western medicine is used in combination with the Qufu Shengji powder, which overcomes the deficiency of healing delay caused by low content of local endogenous growth factor or insufficient receptor. Moreover, Qufu Shengji Powder has a great adhesion effect. It can adhere to the recombinant bovine basic fibroblast growth factor to achieve the purpose of slow release. The effect of fibroblast growth factor was more significant.

Description

Qurot Shengji powder for improving incision healing of lower extremities

technical field

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine preparation for improving poor healing of incisions of lower limbs, and more specifically to a self-made traditional Chinese medicine powder for removing putrefaction and promoting muscle growth combined with external application of recombinant bovine basic fibroblast growth factor to treat poor healing of incisions of lower limbs .

Background technique

In surgical clinical work, some patients will experience fat liquefaction in the surgical incision after surgery, incision oozing, dehiscence or partial dehiscence, and secondary infection. This leads to delayed wound healing. Increased the suffering and economic burden of patients. It also increases the pressure on clinical workers. In the field of cardiac surgery, the great saphenous vein is used as the bypass material in most coronary artery bypass surgery at present. Superficial lymphatic vessels, incision trauma, wound infection, local sensory and motor dysfunction, lymphangitis, swelling of lower extremities, fat liquefaction, dehiscence and other related complications have a high incidence. After the great saphenous vein is removed, the venous return of the lower extremity is blocked, edema, subcutaneous ecchymosis, high tension after edema, and more difficult healing of the skin after denervation, so some patients' lower extremity wounds are poorly healed and cracked. Especially for patients with diabetes, the incidence of postoperative wound complications will be greatly increased.

Modern medicine believes that the main factors affecting incision healing are local and systemic. Among the local factors, postoperative wound infection will damage the cells and matrix, resulting in persistent local inflammation and even suppuration, which is not conducive to tissue repair and wound healing. Matrix junctions are blocked, which in turn affects repair. Local blood circulation disorder can lead to tissue hypoxia, ischemia, or bandage, suture too tight and cause further damage to the tissue, which is also not conducive to healing. Systemic factors are mainly malnutrition. Such as protein, vitamin, iron, zinc and other trace element deficiency or metabolic abnormalities; use of a large number of cell regeneration inhibitors such as corticosteroids; low immune function or systemic serious complications such as multiple organ dysfunction, etc. However, from the perspective of clinical practice, the treatment based on the above understanding, especially the treatment of poor wound healing without postoperative infection, the application of medical therapy such as nutritional supplementation, the effect is not very satisfactory. There is no direct record of poor postoperative incision healing in motherland medicine. However, according to the characteristics of wounds not healing for a long time, it can be confirmed with the long-lasting sores. The regeneration function of the human body is of great significance for wound repair and the prevention, treatment and rehabilitation of many chronic diseases. Ancient medical scientists had a saying that "the disease of ulcers should be treated externally". Therefore, external treatment is the primary and key treatment measure. Traditional Chinese medicine can effectively control infection in the treatment of chronic refractory wound ulcers, promote muscle growth on the basis of removing carrion, improve local blood supply, and regulate its growth factors, which can effectively promote early wound healing. Scholars at home and abroad have increasingly focused on the study of growth factors in the process of wound healing. Because a large number of in vitro and in vivo experiments and clinical experiments have confirmed that growth factors play an important role in the initiation, regulation and control of wound healing. It not only participates in the chemotaxis of inflammatory cells and promotes the proliferation of fibroblasts and vascular endothelial cells and the formation of matrix, but also has an important impact on the later tissue remodeling. New capillaryization and fibroblast proliferation are two elements of granulation tissue formation, and recombinant bovine basic fibroblast growth factor can act on these two links at the same time, indicating that it plays an important role in the formation of wound granulation tissue. However, at present, the effect of using growth factors alone to promote the healing of such wounds is not very satisfactory. Traditional Chinese medicine has definite effects in the treatment of wound healing, but there is still a shortcoming of longer healing time. The invention utilizes self-made Qufu Shengji powder and recombinant bovine basic fibroblast growth factor, and adds exogenous recombinant bovine basic fibroblast growth factor to promote wound healing while removing putrefaction and promoting muscle growth.

Contents of the invention

The technical problem to be solved by the present invention is to provide a traditional Chinese medicine powder for dispelling putrefaction and promoting granulation, combined with the use of western medicine exogenous recombinant bovine basic fibroblast growth factor, which overcomes the problems caused by low content of local endogenous growth factors or insufficient receptors. Healing delays deficiencies. Moreover, Qufu Shengji Powder has a great adhesion effect. It can adhere to the recombinant bovine basic fibroblast growth factor to achieve the purpose of slow release. The effect of fibroblast growth factor was more significant.

In order to solve the above-mentioned technical problems, the present invention provides a kind of Qurot Shengji Powder, whose raw materials include myrrh, frankincense, catechu, calcined keel, calcined pearl, calcined elephant skin, light powder, musk, borneol, dried blood , calamine, yin xiangpi, buffalo horn, white pomegranate, astragalus, mountain white chrysanthemum, burdock seed, chamomile, white wine grass and turmeric.

Wherein, the weights of each raw material in the Qufu Shengji Powder are respectively 28g-32g of myrrh, 28g-32g of frankincense, 4g-8g of catechu, 28g-32g of calcined keel, 4g-8g of calcined pearl, and 4g-8g of calcined elephant skin. 13g~17g, light powder 10g~14g, musk 4g~8g, borneol 4g~8g, dried blood 28g~32g, calamine 13g~17g, yin xiangpi 8g~12g, buffalo horn 18g~22g, white scorpion 16g~ 20g, astragalus 24g-28g, mountain white chrysanthemum 14g-18g, chamomile 10g-14g, liquorice grass 8g-12g and turmeric 11g-15g.

Wherein, the weights of each raw material in the Qufu Shengji Powder are respectively 30g of myrrh, 30g of frankincense, 6g of catechu, 30g of calcined keel, 6g of calcined pearl, 15g of calcined elephant skin, 12g of light powder, 6g of musk, and borneol. 6g, 30g of dried blood, 15g of calamine, 10g of yinxiangpi, 20g of buffalo horn, 18g of white scorpion, 26g of astragalus, 16g of mountain white chrysanthemum, 12g of chamomile, 10g of white wine grass and 13g of turmeric.

The preparation method of the Qufu Shengji Powder is specifically:

In the first step, the yinxiangpi, white pomegranate, astragalus, mountain white chrysanthemum, burdock seed, chaifengchai, baijiucao and turmeric are respectively pulverized into powders, mixed according to the above weight, and the mass of the mixture obtained in this step is 5~ 7 times the alcohol concentration of 60% ~ 80% ethanol reflux extraction 3 times, each 2 hours, combined extracts, filtered; the filtrate was adjusted to pH 5.5 ~ 7 with NaOH solution, let stand, filtered, the filtrate reduced Press to recover ethanol until there is no alcohol smell, let it stand, and centrifuge. The supernatant after centrifugation is concentrated under reduced pressure to remove the solvent to obtain a dry paste, which is crushed into powder;

In the second step, crush myrrh, frankincense, catechu, calcined keel, calcined pearl, calcined elephant skin, light powder, musk, borneol, dried blood, calamine and buffalo horn into powder respectively, and mix according to the above weight, The powder obtained in the first step is further added, stirred and mixed evenly, and the above drugs are dried at about 40°C to 50°C, ground into fine powder, passed through a fine sieve, and autoclaved for later use.

The invention also provides a drug compound for improving the healing of lower extremity incisions, which includes traditional Chinese medicine Qufu Shengji Powder and western medicine recombinant bovine basic fibroblast growth factor.

Wherein, the raw materials of the traditional Chinese medicine Qufu Shengji Powder include myrrh, frankincense, catechu, calcined keel, calcined pearl, calcined elephant skin, light powder, musk, borneol, dried blood, calamine, yinxiangpi , buffalo horn, white pomegranate, astragalus, mountain white chrysanthemum, burdock seed, chamomile, white wine grass and turmeric.

Wherein, the weights of each raw material in the Qufu Shengji Powder are respectively 28g-32g of myrrh, 28g-32g of frankincense, 4g-8g of catechu, 28g-32g of calcined keel, 4g-8g of calcined pearl, and 4g-8g of calcined elephant skin. 13g~17g, light powder 10g~14g, musk 4g~8g, borneol 4g~8g, dried blood 28g~32g, calamine 13g~17g, yin xiangpi 8g~12g, buffalo horn 18g~22g, white scorpion 16g~ 20g, astragalus 24g-28g, mountain white chrysanthemum 14g-18g, chamomile 10g-14g, liquorice grass 8g-12g and turmeric 11g-15g.

Wherein, the weights of each raw material in the Qufu Shengji Powder are respectively 30g of myrrh, 30g of frankincense, 6g of catechu, 30g of calcined keel, 6g of calcined pearl, 15g of calcined elephant skin, 12g of light powder, 6g of musk, and borneol. 6g, 30g of dried blood, 15g of calamine, 10g of yinxiangpi, 20g of buffalo horn, 18g of white scorpion, 26g of astragalus, 16g of mountain white chrysanthemum, 12g of chamomile, 10g of white wine grass and 13g of turmeric.

Beneficial effects of the present invention:

The invention adopts the self-made traditional Chinese medicine powder for removing putrefaction and promoting muscle growth, which has the functions of promoting blood circulation and removing blood stasis, removing putrefaction and promoting muscle regeneration, and clearing heat and detoxifying. It can remove necrotic tissue, rapidly improve the microcirculation of tissue around the wound, increase local blood flow, and promote the rapid growth of residual epithelial tissue. It has a reliable curative effect on various superficial suppurative ulcers, long-term wounds, trauma infections and other diseases. The patient has no pain during the medication process, and the operation is simple and economical. The present invention locally adds western medicine recombinant bovine basic fibroblast growth factor on the basis of traditional Chinese medicine, and overcomes the deficiency of healing delay caused by low content of local endogenous growth factor or insufficient receptor. Moreover, Qufu Shengji Powder has a great adhesion effect. It can adhere to the recombinant bovine basic fibroblast growth factor to achieve the purpose of slow release. The combination of Chinese and Western medicines promotes each other and increases the curative effect.

Detailed ways

The present invention aims at the problems of long healing time and unsatisfactory repairing effect of simple local application of growth factors in the external treatment of lower extremity incision healing by using traditional Chinese medicine, and combined application of self-made Qufu Shengji Powder and recombinant bovine basic fibroblast growth factor improves both curative effects.

The raw materials of the Qufu Shengji Powder include myrrh, frankincense, catechu, calcined keel, calcined pearl, calcined elephant skin, light powder, musk, borneol, dried blood, calamine, yinxiangpi, buffalo horn , white pomegranate, astragalus, mountain white chrysanthemum, burdock seed, chamomile, white wine grass and turmeric.

Further preferably, the Qufu Shengji Powder only consists of the above raw materials.

The weights of each raw material in the Qufu Shengji Powder are respectively 28g-32g of myrrh, 28g-32g of frankincense, 4g-8g of catechu, 28g-32g of calcined keel, 4g-8g of calcined pearl, and 13g-32g of calcined elephant skin. 17g, light powder 10g~14g, musk 4g~8g, borneol 4g~8g, blood jellyfish 28g~32g, calamine 13g~17g, yin xiangpi 8g~12g, buffalo horn 18g~22g, white pomegranate 16g~20g, 24g-28g of astragalus, 14g-18g of mountain white chrysanthemum, 10g-14g of chamomile, 8g-12g of white wine grass and 11g-15g of turmeric.

The weight of each bulk drug in the described Qufu Shengji powder is respectively 30g of myrrh, 30g of frankincense, 6g of catechu, 30g of calcined keel, 6g of calcined pearl, 15g of calcined elephant skin, 12g of light powder, 6g of musk, 6g of borneol, 30g of dried blood, 15g of calamine, 10g of yinxiangpi, 20g of buffalo horn, 18g of white scorpion, 26g of astragalus, 16g of mountain white chrysanthemum, 12g of chamomile, 10g of white wine grass and 13g of turmeric.

The preparation method of the Qufu Shengji Powder is specifically:

In the first step, the yinxiangpi, white pomegranate, astragalus, mountain white chrysanthemum, burdock seed, chaifengchai, baijiucao and turmeric are respectively pulverized into powders, mixed according to the above weight, and the mass of the mixture obtained in this step is 5~ 7 times the alcohol concentration of 60% ~ 80% ethanol reflux extraction 3 times, each 2 hours, combined extracts, filtered; the filtrate was adjusted to pH 5.5 ~ 7 with NaOH solution, let stand, filtered, the filtrate reduced Press to recover ethanol until there is no alcohol smell, let it stand, and centrifuge. The supernatant after centrifugation is concentrated under reduced pressure to remove the solvent to obtain a dry paste, which is crushed into powder;

In the second step, crush myrrh, frankincense, catechu, calcined keel, calcined pearl, calcined elephant skin, light powder, musk, borneol, dried blood, calamine and buffalo horn into powder respectively, and mix according to the above weight, The powder obtained in the first step is further added, stirred and mixed evenly, and the above drugs are dried at about 40°C to 50°C, ground into fine powder, passed through a fine sieve, and autoclaved for later use.

The weight of each bulk drug is respectively:

Making Myrrha: bitter in the mouth, pungent, flat in nature, enters liver, spleen, heart, kidney meridian, has the effect of promoting blood circulation and relieving pain, reducing swelling and promoting granulation. , carbuncle swollen sores, intestinal carbuncle, conjunctival congestion and swelling and pain.

Olibanum: pungent in taste, bitter, warm in nature, enters the heart, liver, and spleen meridians, has the effects of regulating qi and activating blood, relieving pain, and chasing poison. , postpartum blood stasis and tingling and other evidence.

Catechu: bitter in taste, astringent, slightly cold in nature, returns to the lung meridian, has the effect of reducing dampness, promoting granulation and astringing sores, and is used for ulcers, eczema, aphtha, pain from falls, traumatic bleeding and other symptoms.

Calcined keel: sweet, astringent, neutral in nature, enters the heart, liver, kidney, and large intestine meridian, has an important role in calming the nerves, calming the nerves, suppressing sweat and solidifying essence, stopping bleeding and astringent intestines, promoting muscle regeneration and reducing sores. Treatment of convulsions and epilepsy, palpitations and forgetfulness, insomnia and dreaminess, spontaneous sweating, nocturnal emission and stranguria, vomiting blood in the stool, uterine bleeding, dysentery and prolapse of the anus, ulcers that do not close for a long time.

Calcined pearl: sweet, salty, cold in nature, returns to the liver and heart meridian, has the effects of calming the nerves and calming convulsions, improving eyesight and eliminating cloudiness, detoxifying and promoting muscle growth, moisturizing skin and removing freckles, and mainly treating palpitations, insomnia, convulsions, epilepsy, red eyes and cloudy eyes, sores Ulcers are not restrained, skin pigmentation and other certificates.

Calcined Elephant Peel: sweet, salty, warm in nature, has the effect of promoting granulation and astringing sores, and is mainly used to treat sores that persist for a long time.

Light powder: pungent in taste, cold in nature, returns to the large intestine and small intestine meridians, externally used for scabies, stubborn ringworm, ecchymosis, syphilis, sores, eczema; orally used for stagnant phlegm and salivation, edema and swelling, and constipation.

Musk: pungent in taste, warm in nature, heart-guiding, spleen meridian, resuscitating the mind, promoting blood circulation and promoting menstruation, relieving pain, inducing labor.

Borneol: taste pungent, bitter, cool in nature, return to the heart, lung meridian, have the effects of clearing all orifices, dispelling stagnant fire, removing nebula and improving eyesight, reducing swelling and relieving pain. Air-occlusion deafness, sore throat, aphtha, otitis media, carbuncle, hemorrhoids, nebula, pinworms, etc.

Dried blood: sweet, salty, flat in nature, enters the heart, liver, and spleen meridian, orally used to promote blood circulation and dissipate blood stasis, relieve pain, externally used to stop bleeding and promote granulation, astringe sores, and mainly treat congestion, amenorrhea, dysmenorrhea, and postpartum stasis; lumps in the abdomen , stabbing pain in the chest and abdomen; bruises, congestion and swelling; traumatic bleeding, ulcers and other symptoms.

Calamine: the Latin name Calamina, sweet in taste, flat in nature, returns to the Stomach Meridian, has the effect of detoxifying, improving eyesight, reducing nebula, reducing dampness, relieving itching and relieving sores. , Ulcers are not restrained, pus dripping, eczema, skin itching and other symptoms.

Yinxiang Pi: It is the bark of Yinxiang, a plant belonging to the genus Lauraceae. It tastes pungent, slightly sweet, and warm in nature. Diarrhea, loss of appetite, anemofrigid-damp arthralgia, waist and leg pain, bruises, traumatic bleeding and other symptoms.

Buffalo Horn: bitter, salty, cold in nature, four meridians of Guixin, liver, spleen, and stomach, have the effects of clearing away heat, cooling blood, and detoxifying. , Sore throat and pharyngeal swelling and other syndromes, buffalo horn has a good effect of cooling blood and detoxification, promoting blood circulation and removing blood stasis, can improve blood circulation, promote the absorption of exudate, and play the function of removing putrefaction and promoting muscle growth.

Baijiao: Latin name Ampelopsis Radix, bitter in taste, slightly cold in nature, Guixin, Stomach meridian, has the effects of clearing away heat and detoxification, eliminating carbuncle and dissipating stagnation, astringing sores and promoting granulation.

Astragalus: Latin name astragalali radix, sweet in taste, warm in nature, enters the spleen and lung meridians, has the effects of nourishing the spleen soil, elevating yang qi, strengthening the surface and antiperspirant, supporting sores and promoting muscle growth, and it is mainly used to treat fatigue caused by spleen and lung qi deficiency. Less loose stools, palpitations, shortness of breath, sagging of viscera with sunken Qi, metrorrhagia and hematochezia caused by Qi not absorbing blood, chronic diarrhea and prolapse of the anus; spontaneous sweating and night sweats due to deficiency of the exterior, insufficient Qi and blood, etc.

Mountain white chrysanthemum: bitter, pungent, cool in nature, has the effect of clearing away heat and detoxification, eliminating phlegm and cooling blood, mainly treating cold and fever, tonsillitis, bronchitis, hepatitis, enteritis, hot stranguria, carbuncle, swollen furuncle, snake bites and other syndromes .

Burdock Fructus: spicy, bitter; cold in nature, returns to the lungs, stomach meridian, has the effects of dispersing wind-heat, clearing away heat and detoxifying rashes, clearing the lungs, relieving the throat, and dissipating swelling. Hot cough, poor expectoration; sore throat; opaque macule; early stage of measles, poor rash and itching of rubella;

Ganfengchai: It is the leaves of the plant of the Verbenaceae Zizhu plant, which is astringent, slightly pungent, slightly bitter, and flat in nature. It has the effects of dispelling blood stasis and stopping bleeding, detoxifying and reducing swelling. Bruises due to traumatic injuries, hemorrhage due to trauma, burns caused by fire and water, and sores and festers.

Liquor Grass: Bitter in the mouth, pungent, cold in nature, has the effect of clearing away heat and relieving pain, dispelling wind and resolving phlegm, mainly treating laryngitis, pneumonia, sore throat, tonsillitis and other syndromes.

Turmeric: Latin name curcumae longae rhizoma, bitter, pungent, warm in nature, enters the liver meridian, has the effect of breaking blood and promoting qi, stimulating menstruation and relieving pain. Swelling and pain etc.

Studies have shown that growth factors can act as chemoattractants to chemoattract inflammatory cells and tissue repair cells, creating conditions for wound sterilization and later repair; they can also directly act on growth factor receptors on tissue repair cells, and accelerate the growth rate through their mitogenic effect. Cell cycle transformation accelerates wound repair; growth factors can up-regulate the activity of growth factor receptors on tissue repair cells through competitive action, thereby accelerating signal transmission and promoting ulcer wound healing. Recombinant bovine basic fibroblast growth factor is a multifunctional cell growth factor that can promote repair and The role of regeneration. Promote capillary regeneration, improve local blood circulation, and accelerate wound healing, so as to actively promote wound repair and comprehensively improve the quality of wound healing. Non-healing incisions were given exogenous fibroblast growth factor. Recombinant basic fibroblast growth factor can not only promote the repair of refractory wounds, but also in some chronic refractory wounds, the tissue repair cells are often in a "dormant" state, and exogenous application of fibroblast growth factor It can directly act on the specific receptors on its target cells (fibroblasts and vascular endothelial cells, etc.), and cause these cells to divide and proliferate through the mitogenic effect, thereby initiating and accelerating the repair process.

In the present invention, self-made Qurot Shengji Powder, calamine and calamine have the effect of astringing and promoting granulation, and external use can astringe mucous membranes, reduce secretion, and have a good effect on non-healing of sores and ulcers; catechu, calcined pearls, calamine, Buffalo horns can absorb dampness and suppress sores, stop bleeding and promote granulation; Xuejie is sweet, salty and flat in nature, enters the pericardium and liver meridian, has the effects of astringing sores and promoting granulation, promoting blood stasis and relieving pain, and can improve microcirculation. There are different degrees of inhibition; frankincense, musk, liquorice and turmeric have the effect of promoting blood circulation and analgesia, and can inhibit inflammation. Accelerate inflammatory excretion, excretion, absorption, and promote wound healing; myrrh has different degrees of inhibitory effects on a variety of pathogenic fungi, and its antibacterial effect may be related to the content of eugenol, which can reduce swelling and pain, promote blood circulation and promote muscle growth; frankincense and myrrh The medicine is a commonly used medicine for promoting blood circulation and dissipating blood stasis, reducing swelling, relieving pain and promoting muscle growth. Clinically, the two are used in combination to treat chronic refractory ulcers on the body surface; , antibacterial, antiseptic; light powder, white pomegranate, champignon firewood have the magic of dispelling rot and promoting regeneration, and are good medicines for surgery. muscle. The combination of various medicines can promote blood circulation and remove blood stasis, clear away heat and detoxify, promote muscle growth, astringe and eliminate rot, and can promote microcirculation, increase blood supply to wounds, facilitate wound healing, promote liquefaction of necrotic tissue, and promote rapid growth of granulation tissue. To achieve the purpose of curing ulcers, rot, and ulcers that are not healed, it not only has a certain debridement effect, but also has a great adhesion effect. It can adhere to the recombinant bovine basic fibroblast growth factor and achieve the purpose of slow release. It can prolong the action time of recombinant bovine basic fibroblast growth factor and maintain a high concentration locally, which is better than simple use of basic fibroblast growth factor.

Compared with local application of traditional Chinese medicine and growth factor alone to heal poorly healed lower extremity incisions, the self-made Qurot Shengji Powder in the present invention can promote blood circulation and remove blood stasis, clear away heat and detoxify, promote granulation, astringe and remove rot, and at the same time apply recombinant bovine alkaline Fibroblast growth factor, the effects of the two strengthen each other, Qufu Shengji Powder can adhere to recombinant bovine basic fibroblast growth factor, making the local concentration higher and maintaining longer, providing exogenous growth factors for wound healing , promote local blood circulation, accelerate wound healing, actively promote wound repair, and comprehensively improve the quality of healing. It greatly shortens the healing time of poorly healed incisions and improves the quality of healing. The combination of Chinese and Western medicines promotes each other, and the curative effect is better, reducing the suffering of patients and reducing medical expenses.

The present invention also provides a method for improving the healing of lower extremity incisions, the specific steps are:

The first step, incision debridement: For non-healed lower limb incisions, routinely disinfect the surrounding skin with povidone iodine, cut off inactive tissues with scissors, clean the wound surface with 3% hydrogen peroxide, and use 0.9% physiological Repeated flushing with saline to remove exudate and necrotic tissue.

The second step, local application of growth factor: After the incision debridement is completed, the recombinant bovine basic fibroblast growth factor is sprayed locally, and the liquid medicine is directly sprayed on the injured part after debridement.

Step 3: Local application of Qufu Shengji Powder: After debridement is completed, recombinant bovine basic fibroblast growth factor is sprayed locally, and the pre-prepared Qufu Shengji Powder is evenly sprinkled on the incision of the lower limbs and covered with sterile gauze The wound was bandaged and fixed with gauze, and the dressing was changed once a day until the incision healed.

The following examples are used to describe the implementation of the present invention in detail, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention.

Example 1 Qurot Shengji Powder 1

Grind 10g of Yinxiangpi, 18g of Baishui, 26g of Radix Astragali, 16g of Mountain White Chrysanthemum, 12g of Chaifengchai, 10g of Baijiucao and 13g of Curcuma longa into powder, mix well, and use alcohol concentration 6 times the mass of the mixture obtained in this step Reflux extraction with 80% ethanol for 3 times, 2 hours each time, combine the extracts, and filter; adjust the filtrate to pH7 with NaOH solution, let it stand, filter, and recover the ethanol from the filtrate under reduced pressure until it has no alcohol smell, let it stand, and centrifuge Separation, supernatant after centrifugation, concentrated under reduced pressure to remove solvent, obtained dry paste, crushed into powder, prepared 30g of myrrh, 30g of frankincense, 6g of catechu, 30g of calcined keel, 6g of calcined pearl, and 15g of calcined elephant skin , 12g of light powder, 6g of musk, 6g of borneol, 30g of dried blood, 15g of calamine and 20g of buffalo horn are respectively pulverized into powders, mixed, and further added to the powder obtained above, stirred and mixed evenly, and the above drugs are dried at about 45°C, Grind into a fine powder, pass through a fine sieve, and use it for later use after autoclaving to obtain Qurot Shengji Powder 1.

Example 2 Qurot Shengji Powder 2

Grind 8g of yinxiangpi, 16g of white pomegranate, 24g of astragalus, 14g of chrysanthemum, 10g of chaifengchai, 8g of baijiucao and 11g of turmeric into powder, mix well, and use alcohol concentration 6 times the mass of the mixture obtained in this step Reflux extraction with 80% ethanol for 3 times, 2 hours each time, combine the extracts, and filter; adjust the filtrate to pH7 with NaOH solution, let it stand, filter, and recover the ethanol from the filtrate under reduced pressure until it has no alcohol smell, let it stand, and centrifuge Separation, supernatant after centrifugation, concentrated under reduced pressure to remove solvent, obtained dry paste, crushed into powder, prepared 28g of myrrh, 28g of frankincense, 4g of catechu, 28g of calcined keel, 4g of calcined pearl, and 13g of calcined elephant skin , 10g of light powder, 4g of musk, 4g of borneol, 28g of dried blood, 13g of calamine and 18g of buffalo horn were pulverized into powder respectively, mixed, and further added to the powder obtained above, stirred and mixed evenly, and the above medicines were dried at about 45°C, Grind into a fine powder, pass through a fine sieve, and use it for later use after autoclaving to obtain Qurot Shengji Powder 2.

Example 3 Qurot Shengji Powder 3

Grind 12g of Yinxiangpi, 20g of Baixuan, 28g of Radix Astragalus, 18g of Mountain White Chrysanthemum, 14g of Chaifengchai, 12g of Baijiucao and 15g of Turmeric into powder, mix well, and use alcohol concentration 6 times the mass of the mixture obtained in this step Reflux extraction with 80% ethanol for 3 times, 2 hours each time, combine the extracts, and filter; adjust the filtrate to pH7 with NaOH solution, let it stand, filter, and recover the ethanol from the filtrate under reduced pressure until it has no alcohol smell, let it stand, and centrifuge Separation, supernatant after centrifugation, concentrated under reduced pressure to remove solvent, obtained dry paste, crushed into powder, prepared 32g of myrrh, 32g of frankincense, 8g of catechu, 32g of calcined keel, 8g of calcined pearl, and 17g of calcined elephant skin , 14g of light powder, 8g of musk, 8g of borneol, 32g of dried blood, 17g of calamine and 22g of buffalo horn are respectively pulverized into powders, mixed, and further added to the powder obtained above, stirred and mixed evenly, and the above drugs are dried at about 45°C. Grind into a fine powder, pass through a fine sieve, and use it for later use after autoclaving to obtain Qurot Shengji Powder 3.

Embodiment 4 skin allergy test

With the ointment powder 1 prepared by the embodiment of the present invention 1, it is dispersed in water and is configured into a solution of 4g (crude drug)/ml to carry out induction and excitation contact tests, and after 24 hours and 48 hours, the test mice are treated, and no skin is seen. There were erythema and edema, which were not different from those in the negative control group. The results show that the powder has close to zero skin reaction intensity, that is, no skin allergy.

long-term toxicity test

The guinea pigs were randomly divided into 4 groups, 15 in each group. Apply the depilatory agent evenly on both sides of the back spine of the guinea pig, so that the range of depilation is about 40 square centimeters. Clean the depilatory agent, treat each group of guinea pigs after 24 hours and apply respectively the solution 0.2, 0.4 and 0.8ml of the powder 1 configuration prepared by the embodiment of the present invention 1, containing crude drug 92, 184 and 368mg respectively, another group is coated with solvent 0.8ml each Twice a day for 30 consecutive days, the general condition of the guinea pigs was treated, and the animals were sacrificed after the experiment for hematological, blood biochemical and pathological examinations.

No edema, hyperemia, erythema, bleeding spots and ulcers were found in the depilated trunk area of guinea pig trunks in the above three groups of drugs. There was no significant difference between the treatment group and the control group in animal hair color, food intake, and limb activities. Blood biochemical tests showed that the treatment group and the control group were all within the normal range. Histopathological examination showed that there were no obvious lesions in the heart, liver, kidney and local skin of each experimental group. It is suggested that long-term administration of the traditional Chinese medicine powder of the present invention has no obvious toxic effect on local skin and vital organs of the whole body.

Embodiment 5 clinical data

normal information

All 166 patients met the diagnostic criteria of orthopedic incision infection after inspection, and all of them were lower extremity incision infection. There were 83 patients in the control group, including 43 males and 40 females, ranging in age from 21 to 49; 83 patients in the treatment group, including 39 males and 44 females.

treatment method

Treatment group: For non-healed lower limb incisions, routinely disinfect the surrounding skin with povidone iodine, cut off inactive tissues with scissors, clean the wound with 3% hydrogen peroxide, and rinse with 0.9% normal saline repeatedly to remove Exudate and necrotic tissue, spray recombinant bovine basic fibroblast growth factor locally after incision debridement, and spray the liquid directly on the injured area after debridement. After the debridement is completed, after the recombinant bovine basic fibroblast growth factor is sprayed locally, the Qusan Shengji powder 1 prepared in Example 1 prepared in advance is evenly sprinkled on the incision of the lower limbs, and the wound is covered with sterile gauze, wrapped with gauze, Fixed, change the dressing once a day until the incision heals.

Control group: For non-healed lower limb incisions, routinely disinfect the surrounding skin with povidone iodine, cut off inactive tissues with scissors, clean the wound surface with 3% hydrogen peroxide, rinse repeatedly with 0.9% normal saline, and remove Exudate and necrotic tissue, spray recombinant bovine basic fibroblast growth factor locally after incision debridement, and spray the liquid directly on the injured area after debridement. After debridement was completed, recombinant bovine basic fibroblast growth factor was sprayed locally, the wound was covered with sterile gauze, and the gauze was bandaged and fixed. The dressing was changed once a day until the incision healed.

Efficacy evaluation method

Markedly effective: the patient's incision heals better, the condition recovers better, and the incidence of adverse reactions <5%; effective: the patient's incision heals to a certain extent, the condition recovers to a certain extent, and the incidence of adverse reactions <10%; invalid: the patient's incision heals poorly, There was no sign of recovery, and the incidence of adverse reactions was >10%.

Statistical indicators

Statistical software SPSS13.0 was used, the count data was tested by χ2, and the measurement data was Indicates that the t-test was used, and P<0.05 was considered statistically significant.

result

166 patients were followed up for 6 to 8 months. The results are shown in Table 1, Table 2 and Table 3. Patients with allergies, severe infections, and toxic reactions were considered adverse reactions.

The curative effects of the two groups were compared, and the results are shown in Table 1.

Table 1 Comparison of curative effects between the two groups

group Number of cases markedly effective efficient invalid total effective rate therapy group 83 65 17 1 98.8% control group 83 49 25 9 89.2%

As can be seen from Table 1, the use of Qufu Shengji Powder provided by the present invention combined with western medicine recombinant bovine basic fibroblast growth factor has a better effect on the healing of lower limb incisions, and the difference is significant compared with the control group, P<0.05.

The adverse reactions of the two groups of patients were compared, and the results are shown in Table 2.

Table 2 Comparison of adverse reactions between the two groups of patients

group Number of cases allergy severe infection toxic reaction adverse reaction rate therapy group 83 2 0 0 2.41% control group 83 5 2 2 10.85%

As can be seen from Table 2, the use of Qufu Shengji Powder provided by the present invention combined with western medicine recombinant bovine basic fibroblast growth factor for incision healing of lower extremities has less adverse reaction rate, which is significantly different from the control group, P<0.05.

The treatment time of incision infection and the incision healing time were compared, and the results are shown in Table 3.

Table 3 Comparison of incision infection treatment time and incision healing time

group Incision infection treatment time (d) Incision healing time (d) therapy group 5.1±0.3 10.6±1.7 control group 9.5±0.6 17.3±2.2

As can be seen from Table 3, the use of Qufu Shengji Powder provided by the present invention combined with western medicine recombinant bovine basic fibroblast growth factor has shorter treatment time and wound healing time for wound healing of lower extremities.

All of the above-mentioned primary implementations of this intellectual property rights are not intended to limit other forms of implementations of this new product and/or new method. Those skilled in the art will, with this important information, modify the above to achieve a similar implementation. However, all modifications or alterations to the new product based on the present invention belong to reserved rights.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention to other forms. Any skilled person who is familiar with this profession may use the technical content disclosed above to change or modify the equivalent of equivalent changes. Example. However, any simple modifications, equivalent changes and modifications made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still belong to the protection scope of the technical solution of the present invention.

Claims (9)

1. a powder medicine for removing necrotic tissue and promoting muscle growing, is characterized in that: crude drug comprises Myrrha (processed), Olibanum (processed), catechu, Os Draconis (calcined), forges Margarita, forges Corium elephatis, Calomelas, Moschus, Borneolum Syntheticum, Sanguis Draxonis, Calamina, Cortex Cinnamomi Burmannii, Cornu Bubali, Radix Ampelopsis, the Radix Astragali, Herba Asteris Ageratoidis, Fructus Arctii, Folium et Ramulus Callicarpae nudiflorae, Conyza japonica and Rhizoma Curcumae Longae.

2. powder medicine for removing necrotic tissue and promoting muscle growing as claimed in claim 1, is characterized in that: the weight of each crude drug is respectively Myrrha (processed) 28g ~ 32g, Olibanum (processed) 28g ~ 32g, catechu 4g ~ 8g, Os Draconis (calcined) 28g ~ 32g, forges Margarita 4g ~ 8g, forges Corium elephatis 13g ~ 17g, Calomelas 10g ~ 14g, Moschus 4g ~ 8g, Borneolum Syntheticum 4g ~ 8g, Sanguis Draxonis 28g ~ 32g, Calamina 13g ~ 17g, Cortex Cinnamomi Burmannii 8g ~ 12g, Cornu Bubali 18g ~ 22g, Radix Ampelopsis 16g ~ 20g, Radix Astragali 24g ~ 28g, Herba Asteris Ageratoidis 14g ~ 18g, Folium et Ramulus Callicarpae nudiflorae 10g ~ 14g, Conyza japonica 8g ~ 12g and Rhizoma Curcumae Longae 11g ~ 15g.

3. powder medicine for removing necrotic tissue and promoting muscle growing as claimed in claim 1 or 2, is characterized in that: the weight of each crude drug is respectively Myrrha (processed) 30g, Olibanum (processed) 30g, catechu 6g, Os Draconis (calcined) 30g, forges Margarita 6g, forges Corium elephatis 15g, Calomelas 12g, Moschus 6g, Borneolum Syntheticum 6g, Sanguis Draxonis 30g, Calamina 15g, Cortex Cinnamomi Burmannii 10g, Cornu Bubali 20g, Radix Ampelopsis 18g, Radix Astragali 26g, Herba Asteris Ageratoidis 16g, Folium et Ramulus Callicarpae nudiflorae 12g, Conyza japonica 10g and Rhizoma Curcumae Longae 13g.

4. the powder medicine for removing necrotic tissue and promoting muscle growing as described in claims 1 to 3, is characterized in that: the preparation method of described powder medicine for removing necrotic tissue and promoting muscle growing is specially,

The first step, Cortex Cinnamomi Burmannii, Radix Ampelopsis, the Radix Astragali, Herba Asteris Ageratoidis, Fructus Arctii, Folium et Ramulus Callicarpae nudiflorae, Conyza japonica and Rhizoma Curcumae Longae are ground into powder respectively, mix by above-mentioned weight, with the alcohol reflux 3 times of the determining alcohol 60% ~ 80% of the mixture quality obtained relative to this step 5 ~ 7 times, each 2 hours, merge extractive liquid, filters; Filtrate is adjusted to pH5.5 ~ 7 by NaOH solution, leaves standstill, filter, decompression filtrate recycling ethanol, to without alcohol taste, leaves standstill, centrifugalize, the supernatant after centrifugalize, and concentrating under reduced pressure removes solvent, obtains and gets dry extract, be ground into powder;

Second step, by Myrrha (processed), Olibanum (processed), catechu, Os Draconis (calcined), forge Margarita, forge Corium elephatis, Calomelas, Moschus, Borneolum Syntheticum, Sanguis Draxonis, Calamina and Cornu Bubali be ground into powder respectively, mix according to above-mentioned weight, add the powder that the first step obtains further, be uniformly mixed, above medicine is 40 DEG C ~ about 50 DEG C oven dry, and pulverization crosses dusting cover, for subsequent use after autoclave sterilization.

5. for improving a medicinal composition for lower limb wound healing situation, it is characterized in that: comprise Chinese medicine powder medicine for removing necrotic tissue and promoting muscle growing and Western medicine bFGF bovine basic fibroblast growth factor.

6. medicinal composition as claimed in claim 5, is characterized in that: the crude drug of described Chinese medicine powder medicine for removing necrotic tissue and promoting muscle growing comprises Myrrha (processed), Olibanum (processed), catechu, Os Draconis (calcined), forges Margarita, forges Corium elephatis, Calomelas, Moschus, Borneolum Syntheticum, Sanguis Draxonis, Calamina, Cortex Cinnamomi Burmannii, Cornu Bubali, Radix Ampelopsis, the Radix Astragali, Herba Asteris Ageratoidis, Fructus Arctii, Folium et Ramulus Callicarpae nudiflorae, Conyza japonica and Rhizoma Curcumae Longae.

7. the medicinal composition as described in claim 5 or 6, it is characterized in that: in described powder medicine for removing necrotic tissue and promoting muscle growing, the weight of each crude drug is respectively Myrrha (processed) 28g ~ 32g, Olibanum (processed) 28g ~ 32g, catechu 4g ~ 8g, Os Draconis (calcined) 28g ~ 32g, forge Margarita 4g ~ 8g, forge Corium elephatis 13g ~ 17g, Calomelas 10g ~ 14g, Moschus 4g ~ 8g, Borneolum Syntheticum 4g ~ 8g, Sanguis Draxonis 28g ~ 32g, Calamina 13g ~ 17g, Cortex Cinnamomi Burmannii 8g ~ 12g, Cornu Bubali 18g ~ 22g, Radix Ampelopsis 16g ~ 20g, Radix Astragali 24g ~ 28g, Herba Asteris Ageratoidis 14g ~ 18g, Folium et Ramulus Callicarpae nudiflorae 10g ~ 14g, Conyza japonica 8g ~ 12g and Rhizoma Curcumae Longae 11g ~ 15g.

8. the medicinal composition as described in claim 5 to 7, is characterized in that: in described powder medicine for removing necrotic tissue and promoting muscle growing the weight of each crude drug be respectively Myrrha (processed) 30g, Olibanum (processed) 30g, catechu 6g, Os Draconis (calcined) 30g, forge Margarita 6g, forge Corium elephatis 15g, Calomelas 12g, Moschus 6g, Borneolum Syntheticum 6g, Sanguis Draxonis 30g, Calamina 15g, Cortex Cinnamomi Burmannii 10g, Cornu Bubali 20g, Radix Ampelopsis 18g, Radix Astragali 26g, Herba Asteris Ageratoidis 16g, Folium et Ramulus Callicarpae nudiflorae 12g, Conyza japonica 10g and Rhizoma Curcumae Longae 13g.

9. improve a method for lower limb wound healing situation, it is characterized in that, comprising:

The first step, otch debridement: to the lower limb otch of disunion, with povidone iodine routine disinfection surrounding skin, wipe out non-viable tissue with shears, after wound surface is cleaned out, clean wound surface with the hydrogen peroxide of 3% again, repeatedly rinse with 0.9% normal saline, remove sepage and slough;

Second step, topical application somatomedin: localized pulverization bFGF bovine basic fibroblast growth factor after otch debridement, be sprayed directly on to the injury place after debridement by medicinal liquid;

3rd step, topical application powder medicine for removing necrotic tissue and promoting muscle growing: complete in debridement, after localized pulverization bFGF bovine basic fibroblast growth factor, the powder medicine for removing necrotic tissue and promoting muscle growing prepared in advance is evenly spread on lower limb otch, and cover wound with sterile gauze, gauze wrapping, fixing, every day change dressings 1 time, to wound healing.