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CN104621651A - Method for preparing fig vinegar beverage by using multi-strain mixed fermentation - Google Patents

Method for preparing fig vinegar beverage by using multi-strain mixed fermentation Download PDF

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CN104621651A
CN104621651A CN201510036162.5A CN201510036162A CN104621651A CN 104621651 A CN104621651 A CN 104621651A CN 201510036162 A CN201510036162 A CN 201510036162A CN 104621651 A CN104621651 A CN 104621651A
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yeast
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acid bacteria
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CN104621651B (en
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王振斌
刘加友
陈兵兵
马晓珂
刘凤叶
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Jiangsu University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

本发明公开了一种利用多菌种混合发酵制备无花果醋饮料的方法,由以下步骤组成:1)备料:挑选熟透的无花果,除去腐烂果、虫蛀果,经粉碎、护色,得到无花果果浆;2)酶解;3)酵母菌、乳酸菌发酵;4)醋酸发酵;5)过滤;6)调配、杀菌即得无花果醋饮料。本发明采用多菌种混合发酵,在保持无花果营养成分的同时改善产品的风味,产品酸甜可口、色泽纯正、营养丰富、回味绵长、具有无花果特有的果香。本发明制备方法操作简单可行,对于提升无花果精深加工水平,延长产业链,增加无花果产业的经济效益和社会效益具有重要意义。The invention discloses a method for preparing fig vinegar beverage by using multi-strain mixed fermentation, which consists of the following steps: 1) material preparation: selecting ripe figs, removing rotten fruits and moth-eaten fruits, crushing and protecting the color to obtain figs Fruit pulp; 2) enzymatic hydrolysis; 3) yeast and lactic acid bacteria fermentation; 4) acetic acid fermentation; 5) filtration; 6) blending and sterilization to obtain fig vinegar beverage. The invention adopts multi-strain mixed fermentation to improve the flavor of the product while maintaining the nutritional components of the fig, and the product is sweet and sour, pure in color, rich in nutrition, long aftertaste, and has the unique fruity aroma of the fig. The preparation method of the invention is simple and feasible in operation, and has great significance for improving the deep processing level of figs, prolonging the industrial chain, and increasing the economic and social benefits of the fig industry.

Description

一种利用多菌种混合发酵制备无花果醋饮料的方法A method for preparing fig vinegar beverage by using multi-strain mixed fermentation

技术领域                                Technical Fields

本发明属于饮料制备领域,具体涉及一种无花果饮料的制备技术。 The invention belongs to the field of beverage preparation, and in particular relates to a preparation technology of fig beverage.

背景技术 Background technique

无花果是我国一种产量较大的药食兼用的水果,具有很高的营养价值。鲜果除含有丰富的功能多糖、氨基酸类等物质,还有多种脂肪酶、淀粉糖化酶、过氧化物歧化酶(SOD)等,具有润肺止咳、健胃清肠、消肿解毒、增强机体免疫及抑制多种肿瘤的作用。无花果收获季节短且不耐储存,目前主要作为水果消费,不利于无花果精深加工水平的提高和产业链的延长,影响了无花果产业的发展。 Fig is a kind of fruit with a large yield in my country, which is used both as medicine and food, and has high nutritional value. Fresh fruit is not only rich in functional polysaccharides, amino acids and other substances, but also a variety of lipase, starch glucoamylase, superoxide dismutase (SOD), etc., which have the functions of nourishing the lungs and relieving cough, strengthening the stomach and clearing the intestines, reducing swelling and detoxification, and strengthening the body. Immunity and inhibition of a variety of tumor effects. Figs have a short harvest season and are not durable for storage. Currently, they are mainly consumed as fruit, which is not conducive to the improvement of the level of deep processing of figs and the extension of the industrial chain, and affects the development of the fig industry.

醋酸菌饮料是利用酵母菌、醋酸菌等微生物对植物果汁进行充分发酵后的饮品。利用无花果制备果醋,不仅可以调节血液中的酸碱平衡,促进人体对食物中营养物质的吸收,而且醋中的挥发性有机酸和氨基酸等可刺激人的大脑神经,抑制和降低衰老过程中氧化物的形成。而且还能吸收无花果中丰富的营养 Acetic acid bacteria drink is a drink that uses microorganisms such as yeast and acetic acid bacteria to fully ferment plant juice. Using figs to prepare fruit vinegar can not only regulate the acid-base balance in the blood, and promote the body's absorption of nutrients in food, but also the volatile organic acids and amino acids in vinegar can stimulate the brain nerves of people, inhibit and reduce the aging process. oxide formation. And it can also absorb the rich nutrients in figs

经检索中国专利号CN 103897975 A“前液后固浇淋式无花果醋及其制备工艺公布了一种无花果醋的制备方法,以无花果为原料经过发酵前处理阶段、酒化发酵处理阶段、醋化发酵处理阶段、淋醋处理阶段和澄清处理阶段等六个阶段的制备得到一种无花果醋。其不足在于无花果破碎过程中容易造成浆汁的褐变,影响产品的色泽;操作过程复杂,生产周期较长,生产成本高。 After retrieving Chinese Patent No. CN 103897975 A "Pre-liquid and then solid pouring type fig vinegar and its preparation process", a preparation method of fig vinegar has been published. Using fig as raw material, it goes through the pre-fermentation treatment stage, wine fermentation treatment stage, vinegarization A kind of fig vinegar is obtained by the preparation of six stages such as the fermentation treatment stage, the vinegar treatment stage and the clarification treatment stage. Its disadvantage is that the browning of the juice is easily caused in the fig crushing process, which affects the color of the product; the operation process is complicated and the production cycle Long, high production costs.

发明内容 Contents of the invention

本发明的目的是解决上述现有技术的不足,提供一种利用多菌种混合发酵制备无花果醋饮料的方法,增加无花果果汁饮品的种类。本发明制备的无花果醋酸甜可口、色泽纯正、营养丰富、回味绵长、具有无花果特有的果香。 The purpose of the present invention is to solve the above-mentioned deficiencies in the prior art, provide a method for preparing fig vinegar beverage by using multi-strain mixed fermentation, and increase the types of fig fruit juice beverage. The fig vinegar prepared by the invention is sweet and sour, delicious, pure in color, rich in nutrition, long aftertaste, and has the unique fruity aroma of figs.

为了解决以上技术问题,本发明采用的具体技术方案如下: In order to solve the above technical problems, the concrete technical scheme that the present invention adopts is as follows:

一种利用多菌种混合发酵制备无花果醋饮料的方法,其特征在于包括以下步骤: A method for preparing fig vinegar beverage by multi-species mixed fermentation is characterized in that it comprises the following steps:

步骤一,备料:挑选熟透的无花果,除去腐烂果、虫蛀果,得到优质无花果;将水、Vc和NaCl以质量比100:(0.1-0.4):(0.3-0.5)充分混合,组成护色溶液;将所述优质无花果经粉碎后和所述护色溶液以质量比为1:(3-8)进行混合得到无花果果浆; Step 1, material preparation: select ripe figs, remove rotten fruits and moth-eaten fruits to obtain high-quality figs; fully mix water, Vc and NaCl at a mass ratio of 100: (0.1-0.4): (0.3-0.5) to form a protective Color solution; the high-quality figs are pulverized and mixed with the color protection solution at a mass ratio of 1: (3-8) to obtain fig pulp;

步骤二,酶解:将所述无花果果浆加入发酵罐,然后向所述发酵罐中加入果胶酶,每100g的无花果果浆配0.3-0.5mg的果胶酶,在温度为40-50℃、pH在2.5-4.5的条件下酶解2-4小时;得到无花果发酵液; Step 2, enzymatic hydrolysis: add the fig pulp into a fermenter, then add pectinase to the fermenter, add 0.3-0.5 mg of pectinase per 100 g of fig pulp, and heat the mixture at a temperature of 40-50 Enzymolysis for 2-4 hours under the conditions of ℃ and pH 2.5-4.5; fig fermentation broth is obtained;

步骤三,酵母菌、乳酸菌发酵:将酵母菌和乳酸菌以(1-3):1的质量比混合后加入到所述无花果发酵液,在30-45℃的条件下恒温静置48-96小时后,终止发酵,得到无花果发酵繆液;所述酵母菌和乳酸菌总质量与无花果发酵液的质量比为0.6%-1.0%; Step 3, fermentation of yeast and lactic acid bacteria: mix yeast and lactic acid bacteria at a mass ratio of (1-3): 1, add them to the fig fermentation broth, and stand at a constant temperature of 30-45°C for 48-96 hours Afterwards, stop the fermentation to obtain fig fermentation liquid; the mass ratio of the total mass of yeast and lactic acid bacteria to the fig fermentation liquid is 0.6%-1.0%;

步骤四,醋酸发酵:在所述无花果发酵繆液中接入醋酸菌进行醋酸发酵, 发酵时间为48-72h,发酵温度为35-40℃,得醋酸繆液;所述醋酸菌与无花果发酵繆液的质量比为0.5%-1.0%; Step 4, acetic acid fermentation: insert acetic acid bacteria into the fig fermentation solution to carry out acetic acid fermentation, the fermentation time is 48-72h, and the fermentation temperature is 35-40°C to obtain acetic acid solution; the acetic acid bacteria and fig fermentation solution The mass ratio of liquid is 0.5%-1.0%;

步骤五,过滤:将所述醋酸繆液用微孔过滤器进行过滤,过滤孔径为0.6-1.0微米; Step 5, filtering: filtering the acetic acid solution with a microporous filter, the filter aperture is 0.6-1.0 microns;

步骤六,调配、杀菌:将水、醋酵繆液、蔗糖、蜂蜜、无花果香精依质量比为100:(30-50):(6-10):(2-4):(0.1-0.2)的比例混合均匀,然后迅速加热到75-85℃并保持15-20min即得无花果醋饮料。 Step 6, deployment and sterilization: mix water, vinegar yeast solution, sucrose, honey, and fig essence in a mass ratio of 100: (30-50): (6-10): (2-4): (0.1-0.2) The ratio is mixed evenly, then rapidly heated to 75-85° C. and maintained for 15-20 minutes to obtain the fig vinegar beverage.

所述步骤一中:护色溶液中水、Vc和NaCl的质量比优选为100:0.3:0.4;优质无花果和护色溶液的质量比优选为1:6。 In the first step: the mass ratio of water, V c and NaCl in the color-protecting solution is preferably 100:0.3:0.4; the mass ratio of high-quality figs and color-protecting solution is preferably 1:6.

于所述步骤二中温度优选45℃,pH优选4.0,时间优选3h,果胶酶与无花果果浆的料液比优选为0.4 mg:100g。 In the second step, the temperature is preferably 45°C, the pH is preferably 4.0, the time is preferably 3 hours, and the solid-liquid ratio of pectinase to fig pulp is preferably 0.4 mg:100 g.

所述步骤三中:酵母菌和乳酸菌的接种比例优选2:1,发酵时间优选68h,发酵温度优选35℃;酵母菌和乳酸菌的总质量与无花果发酵液质量比优选0.8%;所述酵母为安琪葡萄酒·果酒专用酵母。 In said step three: the inoculation ratio of yeast and lactic acid bacteria is preferably 2:1, the fermentation time is preferably 68h, and the fermentation temperature is preferably 35°C; the total mass of yeast and lactic acid bacteria is preferably 0.8% to the mass ratio of fig fermentation broth; the yeast is Angel wine and fruit wine special yeast.

所述步骤四中:醋酸菌与无花果发酵液的质量比优选0.6%,发酵时间优选56h,发酵温度优选38℃,所用醋酸菌为上海佳民酿醋醋酸菌。 In the fourth step: the mass ratio of acetic acid bacteria to fig fermentation broth is preferably 0.6%, the fermentation time is preferably 56 hours, and the fermentation temperature is preferably 38°C.

所述步骤五中:将所述发酵液用微孔过滤器进行过滤,过滤孔径为0.8微米。 In the fifth step: filtering the fermented liquid with a microporous filter with a filter pore size of 0.8 microns.

所述步骤六中:水、发酵繆液、蔗糖、蜂蜜、无花果香精的质量比优选100:40:8:3:0.15,杀菌温度优选80℃,时间优选18min。本发明具有有益效果。 In said step six: the mass ratio of water, fermentation liquid, sucrose, honey, and fig essence is preferably 100:40:8:3:0.15, the sterilization temperature is preferably 80° C., and the time is preferably 18 minutes. The invention has beneficial effects.

1.本发明采用酵母菌、乳酸菌和醋酸菌纯种混菌发酵,丰富了无花果饮品的种类。 1. The present invention adopts yeast, lactic acid bacteria and acetic acid bacteria pure-bred mixed bacteria fermentation, enriches the kind of fig drink.

2.本发明采用多菌种混菌发酵,优化了益生菌发酵的效果,饮品色泽纯正、营养丰富、回味绵长,具备降血压、血脂、抗疲劳、润肺止咳、健胃清肠、消肿解毒、增强机体免疫及抑制多种肿瘤的作用 2. The present invention uses multi-strain mixed fermentation to optimize the effect of probiotic fermentation. The drink has pure color, rich nutrition, and long aftertaste. Detoxification, enhance the body's immunity and inhibit the role of a variety of tumors

3.本发明操作简单可行,有利于提高无花果的附加值,延长无花果产业链,对农民创收,增加社会经济效益具有重要意义。 3. The operation of the present invention is simple and feasible, is beneficial to increase the added value of figs, prolongs the industrial chain of figs, and is of great significance for farmers to generate income and increase social and economic benefits.

具体实施方式 Detailed ways

下面结合具体实施例,对本发明的技术方案做进一步详细说明。 The technical solutions of the present invention will be described in further detail below in conjunction with specific embodiments.

实施例1 Example 1

1)备料:挑选熟透的无花果,除去腐烂果、虫蛀果,得到优质无花果;在3000g水中加入3g的Vc和9g NaCl充分混合,组成护色溶液; 1000g无花果和3000g护色液充分粉碎混匀得到无花果果浆; 1) Preparation: Select ripe figs, remove rotten fruits and moth-eaten fruits to obtain high-quality figs; add 3g Vc and 9g NaCl to 3000g water and mix thoroughly to form a color protection solution; 1000g figs and 3000g color protection solution are fully crushed and mixed Evenly obtain fig pulp;

2)酶解:将所述无花果果浆加入发酵罐,然后向所述发酵罐中加入12mg果胶酶,在温度为40℃、pH在2.5的条件下酶解2小时,得到无花果发酵液; 2) Enzymolysis: adding the fig pulp into a fermenter, then adding 12 mg of pectinase into the fermenter, and enzymatically hydrolyzing it for 2 hours at a temperature of 40°C and a pH of 2.5 to obtain a fig fermentation liquid;

3)酵母菌、乳酸菌发酵:向所述的无花果发酵液各加入12g酵母菌和乳酸菌,在30℃的条件下恒温静置48小时后,终止发酵,得到无花果发酵繆液; 3) Fermentation of yeast and lactic acid bacteria: adding 12 g of yeast and lactic acid bacteria to the fig fermentation liquid, standing at a constant temperature at 30° C. for 48 hours, and then terminating the fermentation to obtain a fig fermentation liquid;

 4)醋酸发酵:在所述无花果发酵繆液中接入20g醋酸菌进行醋酸发酵, 发酵时间为48h,发酵温度为35℃,得醋酸繆液,得到的醋酸繆液总酸度为12%; 4) Acetic acid fermentation: insert 20g of acetic acid bacteria into the fig fermentation liquid to carry out acetic acid fermentation, the fermentation time is 48h, the fermentation temperature is 35°C, to obtain acetic acid liquid, the total acidity of the obtained acetic acid liquid is 12%;

5)过滤:将所述醋酸繆液用微孔过滤器进行过滤,过滤孔径为0.6微米,得到300g残渣和3650g无花果醋酸滤液。 5) Filtration: Filter the fig acetic acid solution with a microporous filter with a filter aperture of 0.6 micron to obtain 300 g of residue and 3650 g of fig acetic acid filtrate.

6)调配、杀菌:向无花果醋酸滤液中加入12166g水、730g蔗糖、243.34g蜂蜜和12.17g无花果香精,然后迅速加热到75℃并保持15min,得无花果醋饮料。本产品饮品色泽纯正、营养丰富、回味绵长。 6) Blending and sterilization: Add 12166g of water, 730g of sucrose, 243.34g of honey and 12.17g of fig essence to the fig acetic acid filtrate, then rapidly heat to 75°C and keep it for 15min to obtain a fig vinegar beverage. The drink of this product has pure color, rich nutrition and long aftertaste.

实施例2: 1)备料:挑选熟透的无花果,除去腐烂果、虫蛀果,得到优质无花果;在8000g水中加入32g的Vc和40g NaCl充分混合,组成护色溶液; 1000g无花果和8000g护色液充分粉碎混匀得到无花果果浆; Embodiment 2: 1) Preparation of materials: select ripe figs, remove rotten fruits and moth-eaten fruits to obtain high-quality figs; add 32g of Vc and 40g NaCl to 8000g of water and fully mix to form a color-protecting solution; 1000g of figs and 8000g of color-protecting The liquid is fully pulverized and mixed to obtain fig pulp;

2)酶解:将所述无花果果浆加入发酵罐,然后向所述发酵罐中加入45mg果胶酶,在温度为50℃、pH在4.5的条件下酶解4小时;得到无花果发酵液; 2) Enzymolysis: add the fig pulp into a fermenter, then add 45 mg of pectinase into the fermenter, and enzymolyze it for 4 hours at a temperature of 50°C and a pH of 4.5; obtain a fig fermented liquid;

3)酵母菌、乳酸菌发酵:向无花果发酵液加入67.5g酵母菌和22.5g乳酸菌在45℃的条件下恒温静置96小时后,终止发酵,得到无花果发酵繆液; 3) Saccharomycete, lactic acid bacteria fermentation: add 67.5g yeast and 22.5g lactic acid bacteria to the fig fermentation broth, leave it at a constant temperature for 96 hours under the condition of 45°C, stop the fermentation, and obtain the fig fermentation broth;

4)醋酸发酵:在所述无花果发酵繆液中接入90g醋酸菌进行醋酸发酵, 发酵时间为72h,发酵温度为40℃,得醋酸繆液;得到的醋酸繆液酸度 10%; 4) Acetic acid fermentation: insert 90g of acetic acid bacteria into the fig fermentation liquid to carry out acetic acid fermentation, the fermentation time is 72h, and the fermentation temperature is 40°C to obtain acetic acid liquid; the acidity of the obtained acetic acid liquid is 10%;

5)过滤:将所述醋酸繆液用微孔过滤器进行过滤,过滤孔径为1.0微米得到280g湿残渣和8720g无花果醋酸滤液; 5) Filtration: The acetic acid solution was filtered with a microporous filter with a filter aperture of 1.0 micron to obtain 280 g of wet residue and 8720 g of fig acetic acid filtrate;

6)调配、杀菌:向无花果醋酸滤液中加入17440g水、1744g蔗糖、697.6g蜂蜜和34.88g无花果香精,然后迅速加热到75℃并保持15min,得无花果醋饮料。本产品饮品色泽纯正、营养丰富、回味绵长。 6) Blending and sterilization: Add 17440g water, 1744g sucrose, 697.6g honey and 34.88g fig essence to the fig acetic acid filtrate, then rapidly heat to 75°C and keep it for 15min to obtain a fig vinegar drink. The drink of this product has pure color, rich nutrition and long aftertaste.

实施例3: Example 3:

1)备料:挑选熟透的无花果,除去腐烂果、虫蛀果,得到优质无花果;在6000g水中加入18gVc和24gNaCl充分混合,组成护色溶液;1000g无花果与6000g护色液混合得到无花果果浆; 1) Preparation: select ripe figs, remove rotten fruits and moth-eaten fruits to obtain high-quality figs; add 18gVc and 24gNaCl to 6000g water and mix thoroughly to form a color protection solution; mix 1000g figs with 6000g color protection solution to obtain fig pulp;

2)酶解:将所述无花果果浆加入发酵罐,然后向所述发酵罐中加入28mg果胶酶,在温度为45℃、pH在4.0的条件下酶解3小时;得到无花果发酵液; 2) Enzymolysis: adding the fig pulp into a fermenter, then adding 28 mg of pectinase into the fermenter, and enzymatically hydrolyzing it for 3 hours at a temperature of 45°C and a pH of 4.0 to obtain a fig fermented liquid;

3)酵母菌、乳酸菌发酵:向无花果发酵液加入37.34g酵母菌和和18.66g乳酸菌,在35℃的条件下恒温静置68小时后,终止发酵,得到无花果发酵繆液;                                                         3) Fermentation of yeast and lactic acid bacteria: add 37.34g of yeast and 18.66g of lactic acid bacteria to the fig fermentation liquid, and after standing at a constant temperature at 35°C for 68 hours, stop the fermentation to obtain a fig fermentation liquid;

4)醋酸发酵:在所述无花果发酵繆液中接入42g醋酸菌进行醋酸发酵, 发酵时间为56h,发酵温度为38℃,得醋酸繆液;得到的醋酸繆液酸度为15%。 4) Acetic acid fermentation: insert 42g of acetic acid bacteria into the fig fermentation liquid to carry out acetic acid fermentation, the fermentation time is 56h, and the fermentation temperature is 38°C to obtain acetic acid liquid; the acidity of the obtained acetic acid liquid is 15%.

5)过滤:将所述醋酸繆液用微孔过滤器进行过滤,过滤孔径为0.8微米,得到275g湿残渣和6725g无花果滤液; 5) Filtration: The acetic acid solution was filtered with a microporous filter with a filter aperture of 0.8 microns to obtain 275g of wet residue and 6725g of fig filtrate;

6)调配、杀菌:向无花果醋酸滤液中加入16812.5g水、1345g蔗糖、504.38g蜂蜜和25.21g无花果香精,然后迅速加热到75℃并保持15min,得无花果醋饮料。本产品饮品色泽纯正、营养丰富、回味绵长。 6) Blending and sterilization: Add 16812.5g water, 1345g sucrose, 504.38g honey and 25.21g fig essence to the fig acetic acid filtrate, then rapidly heat to 75°C and keep it for 15min to obtain a fig vinegar beverage. The drink of this product has pure color, rich nutrition and long aftertaste.

Claims (7)

1. 一种利用多菌种混合发酵制备无花果醋饮料的方法,其特征在于包括以下步骤: 1. a kind of method that utilizes multi-species mixed fermentation to prepare fig vinegar beverage, it is characterized in that comprising the following steps: 步骤一,备料:挑选熟透的无花果,除去腐烂果、虫蛀果,得到优质无花果;将水、Vc和NaCl以质量比100:(0.1-0.4):(0.3-0.5)充分混合,组成护色溶液;将所述优质无花果经粉碎后和所述护色溶液以质量比为1:(3-8)进行混合得到无花果果浆; Step 1, material preparation: select ripe figs, remove rotten fruits and moth-eaten fruits to obtain high-quality figs; fully mix water, Vc and NaCl at a mass ratio of 100: (0.1-0.4): (0.3-0.5) to form a protective Color solution; the high-quality figs are pulverized and mixed with the color protection solution at a mass ratio of 1: (3-8) to obtain fig pulp; 步骤二,酶解:将所述无花果果浆加入发酵罐,然后向所述发酵罐中加入果胶酶,每100g的无花果果浆配0.3-0.5mg的果胶酶,在温度为40-50℃、pH在2.5-4.5的条件下酶解2-4小时;得到无花果发酵液; Step 2, enzymatic hydrolysis: add the fig pulp into a fermenter, then add pectinase to the fermenter, add 0.3-0.5 mg of pectinase per 100 g of fig pulp, and heat the mixture at a temperature of 40-50 Enzymolysis for 2-4 hours under the conditions of ℃ and pH 2.5-4.5; fig fermentation broth is obtained; 步骤三,酵母菌、乳酸菌发酵:将酵母菌和乳酸菌以(1-3):1的质量比混合后加入到所述无花果发酵液,在30-45℃的条件下恒温静置48-96小时后,终止发酵,得到无花果发酵繆液;所述酵母菌和乳酸菌总质量与无花果发酵液的质量比为0.6%-1.0%; Step 3, fermentation of yeast and lactic acid bacteria: mix yeast and lactic acid bacteria at a mass ratio of (1-3): 1, add them to the fig fermentation broth, and stand at a constant temperature of 30-45°C for 48-96 hours Afterwards, stop the fermentation to obtain fig fermentation liquid; the mass ratio of the total mass of yeast and lactic acid bacteria to the fig fermentation liquid is 0.6%-1.0%; 步骤四,醋酸发酵:在所述无花果发酵繆液中接入醋酸菌进行醋酸发酵, 发酵时间为48-72h,发酵温度为35-40℃,得醋酸繆液;所述醋酸菌与无花果发酵繆液的质量比为0.5%-1.0%; Step 4, acetic acid fermentation: insert acetic acid bacteria into the fig fermentation solution to carry out acetic acid fermentation, the fermentation time is 48-72h, and the fermentation temperature is 35-40°C to obtain acetic acid solution; the acetic acid bacteria and fig fermentation solution The mass ratio of liquid is 0.5%-1.0%; 步骤五,过滤:将所述醋酸繆液用微孔过滤器进行过滤,过滤孔径为0.6-1.0微米; Step 5, filtering: filtering the acetic acid solution with a microporous filter, the filter aperture is 0.6-1.0 microns; 步骤六,调配、杀菌:将水、醋酵繆液、蔗糖、蜂蜜、无花果香精依质量比为100:(30-50):(6-10):(2-4):(0.1-0.2)的比例混合均匀,然后迅速加热到75-85℃并保持15-20min即得无花果醋饮料。 Step 6, deployment and sterilization: mix water, vinegar yeast solution, sucrose, honey, and fig essence in a mass ratio of 100: (30-50): (6-10): (2-4): (0.1-0.2) The ratio is mixed evenly, then rapidly heated to 75-85° C. and maintained for 15-20 minutes to obtain the fig vinegar beverage. 2.根据权利要求1所述的一种利用多菌种混合发酵制备无花果醋饮料的制备方法,其特征在于所述步骤一中:护色溶液中水、Vc和NaCl的质量比优选为100:0.3:0.4;优质无花果和护色溶液的质量比优选为1:6。 2. A kind of preparation method utilizing multi-strain mixed fermentation to prepare fig vinegar beverage according to claim 1, is characterized in that in said step one: in the color protection solution, the mass ratio of water, Vc and NaCl is preferably 100 :0.3:0.4; the mass ratio of high-quality figs and color-protecting solution is preferably 1:6. 3.根据权利要求1所述的一种利用多菌种混合发酵制备无花果醋饮料的制备方法,其特征在于所述步骤二中温度优选45℃,pH优选4.0,时间优选3h,果胶酶与无花果果浆的料液比优选为0.4 mg:100g。 3. a kind of preparation method utilizing multi-strain mixed fermentation to prepare fig vinegar beverage according to claim 1, it is characterized in that in described step 2, temperature is preferably 45 DEG C, pH is preferably 4.0, time is preferably 3h, pectinase and The solid-liquid ratio of fig pulp is preferably 0.4 mg:100g. 4.根据权利要求1所述的一种利用多菌种混合发酵制备无花果醋饮料的制备方法,其特征在于所述步骤三中:酵母菌和乳酸菌的接种比例优选2:1,发酵时间优选68h,发酵温度优选35℃;酵母菌和乳酸菌的总质量与无花果发酵液质量比优选0.8%;所述酵母为安琪葡萄酒·果酒专用酵母。 4. A kind of preparation method utilizing multi-species mixed fermentation to prepare fig vinegar beverage according to claim 1, characterized in that in said step 3: the inoculation ratio of yeast and lactic acid bacteria is preferably 2:1, and the fermentation time is preferably 68h , the fermentation temperature is preferably 35°C; the ratio of the total mass of yeast and lactic acid bacteria to the mass of fig fermentation broth is preferably 0.8%; the yeast is special yeast for Angel wine and fruit wine. 5.根据权利要求1所述的一种利用无花果乳酸混菌发酵功能饮料的制备方法,其特征在于所述步骤四中:醋酸菌与无花果发酵液的质量比优选0.6%,发酵时间优选56h,发酵温度优选38℃,所用醋酸菌为上海佳民酿醋醋酸菌。 5. A method for preparing functional beverages fermented by fig lactic acid mixed bacteria according to claim 1, characterized in that in step 4: the mass ratio of acetic acid bacteria to fig fermentation liquid is preferably 0.6%, and the fermentation time is preferably 56h, The fermentation temperature is preferably 38°C, and the acetic acid bacteria used are Shanghai Jiamin Acetobacter. 6.根据权利要求1所述的一种利用无花果乳酸混菌发酵功能饮料的制备方法,其特征在于所述步骤五中:将所述发酵液用微孔过滤器进行过滤,过滤孔径为0.8微米。 6. A method for preparing functional beverages fermented by fig lactic acid mixed bacteria according to claim 1, characterized in that in step five: filtering the fermented liquid with a microporous filter with a filter aperture of 0.8 microns . 7.根据权利要求1所述的一种利用无花果乳酸混菌发酵功能饮料的制备方法,其特征在于所述步骤六中:水、发酵繆液、蔗糖、蜂蜜、无花果香精的质量比优选100:40:8:3:0.15,杀菌温度优选80℃,时间优选18min。 7. a kind of preparation method utilizing fig lactic acid mixed bacteria fermented functional beverage according to claim 1, is characterized in that in described step 6: the mass ratio of water, fermented liquor, sucrose, honey, fig essence is preferably 100: 40:8:3:0.15, the sterilization temperature is preferably 80°C, and the sterilization time is preferably 18 minutes.
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CN108450726A (en) * 2018-03-12 2018-08-28 大连军门保健食品有限公司 A kind of multi-strain fermentation type brown sugar vinegar beverage and preparation method thereof
CN109770139A (en) * 2019-01-29 2019-05-21 四川久润泰科技有限公司 A kind of low alcohol fermented fig beverage and preparation method thereof
CN111617218A (en) * 2019-02-27 2020-09-04 大江生医股份有限公司 Fat-burning fermented product and its preparation method and use for preparing a fat-burning and weight-loss composition
CN110004022A (en) * 2019-05-13 2019-07-12 云南农业大学 Preparation method of thick-stemmed thick plum fruit vinegar based on mixed fermentation
CN112094725A (en) * 2020-09-08 2020-12-18 山东药品食品职业学院 Preparation method of fermented fig fruit vinegar
CN112342111A (en) * 2020-11-17 2021-02-09 黄健 A kind of preparation method of jasmine tea vinegar beverage

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