CN104621357A - Novel liquid feed additive and preparation method thereof - Google Patents
Novel liquid feed additive and preparation method thereof Download PDFInfo
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- CN104621357A CN104621357A CN201410792051.2A CN201410792051A CN104621357A CN 104621357 A CN104621357 A CN 104621357A CN 201410792051 A CN201410792051 A CN 201410792051A CN 104621357 A CN104621357 A CN 104621357A
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- 239000007788 liquid Substances 0.000 title claims abstract description 43
- 239000003674 animal food additive Substances 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 241000894006 Bacteria Species 0.000 claims abstract description 42
- 150000001875 compounds Chemical class 0.000 claims abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims description 31
- 230000001580 bacterial effect Effects 0.000 claims description 28
- 239000002609 medium Substances 0.000 claims description 28
- 238000009472 formulation Methods 0.000 claims description 17
- 230000004913 activation Effects 0.000 claims description 15
- 230000000975 bioactive effect Effects 0.000 claims description 15
- 244000068988 Glycine max Species 0.000 claims description 13
- 235000010469 Glycine max Nutrition 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 13
- 239000007864 aqueous solution Substances 0.000 claims description 11
- 239000011573 trace mineral Substances 0.000 claims description 11
- 235000013619 trace mineral Nutrition 0.000 claims description 11
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 claims description 10
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 10
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 10
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 10
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 10
- 241000191998 Pediococcus acidilactici Species 0.000 claims description 9
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 8
- 241000194032 Enterococcus faecalis Species 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 8
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 239000011790 ferrous sulphate Substances 0.000 claims description 7
- 239000002054 inoculum Substances 0.000 claims description 7
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 7
- 238000010899 nucleation Methods 0.000 claims description 7
- 230000000243 photosynthetic effect Effects 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 241000235342 Saccharomycetes Species 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 229930003451 Vitamin B1 Natural products 0.000 claims description 5
- 229960004050 aminobenzoic acid Drugs 0.000 claims description 5
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 5
- 239000004327 boric acid Substances 0.000 claims description 5
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims description 5
- 235000002867 manganese chloride Nutrition 0.000 claims description 5
- BZDIAFGKSAYYFC-UHFFFAOYSA-N manganese;hydrate Chemical compound O.[Mn] BZDIAFGKSAYYFC-UHFFFAOYSA-N 0.000 claims description 5
- 229910000480 nickel oxide Inorganic materials 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 235000015393 sodium molybdate Nutrition 0.000 claims description 5
- 229960003495 thiamine Drugs 0.000 claims description 5
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 5
- 235000010374 vitamin B1 Nutrition 0.000 claims description 5
- 239000011691 vitamin B1 Substances 0.000 claims description 5
- 239000011592 zinc chloride Substances 0.000 claims description 5
- 235000005074 zinc chloride Nutrition 0.000 claims description 5
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 4
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 229930003779 Vitamin B12 Natural products 0.000 claims description 4
- 229930003537 Vitamin B3 Natural products 0.000 claims description 4
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 239000011565 manganese chloride Substances 0.000 claims description 4
- 229940099607 manganese chloride Drugs 0.000 claims description 4
- 229960003512 nicotinic acid Drugs 0.000 claims description 4
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 4
- GNRSAWUEBMWBQH-UHFFFAOYSA-N oxonickel Chemical compound [Ni]=O GNRSAWUEBMWBQH-UHFFFAOYSA-N 0.000 claims description 4
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims description 4
- 239000011684 sodium molybdate Substances 0.000 claims description 4
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 4
- 235000019163 vitamin B12 Nutrition 0.000 claims description 4
- 239000011715 vitamin B12 Substances 0.000 claims description 4
- 235000019160 vitamin B3 Nutrition 0.000 claims description 4
- 239000011708 vitamin B3 Substances 0.000 claims description 4
- 235000019158 vitamin B6 Nutrition 0.000 claims description 4
- 239000011726 vitamin B6 Substances 0.000 claims description 4
- 229940011671 vitamin b6 Drugs 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 3
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 238000009630 liquid culture Methods 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 230000006378 damage Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract 1
- 239000008213 purified water Substances 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 description 7
- 238000011109 contamination Methods 0.000 description 4
- 241000238557 Decapoda Species 0.000 description 2
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- 235000013601 eggs Nutrition 0.000 description 2
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- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- HGUFODBRKLSHSI-UHFFFAOYSA-N 2,3,7,8-tetrachloro-dibenzo-p-dioxin Chemical compound O1C2=CC(Cl)=C(Cl)C=C2OC2=C1C=C(Cl)C(Cl)=C2 HGUFODBRKLSHSI-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 235000005744 Bacillus subtilis subsp subtilis Nutrition 0.000 description 1
- 241000948854 Bacillus subtilis subsp. subtilis Species 0.000 description 1
- 206010059410 Faecaluria Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000353204 Girella Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
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- 150000001413 amino acids Chemical class 0.000 description 1
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- 238000006243 chemical reaction Methods 0.000 description 1
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- OPXKTCUYRHXSBK-UHFFFAOYSA-N clenbuterol hydrochloride Chemical compound Cl.CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 OPXKTCUYRHXSBK-UHFFFAOYSA-N 0.000 description 1
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- Fodder In General (AREA)
Abstract
The invention discloses a novel liquid feed additive and a preparation method of the liquid feed additive and belongs to the technical field of feed additives. The feed additive is prepared from the following raw materials in parts by weight: 10-12 parts of a compound biological live bacteria agent, 18-20 parts of a nutrient solution and 65-68 parts of purified water. The compound biological live bacteria agent is independently developed; by virtue of the application of the feed additive prepared by the method, the damage to the ecological environment is avoided, and the environment can be effectively purified and protected; a pollution-free safe feed is provided for a large number of farmers, thereby ensuring the human life and health.
Description
Technical field
The invention belongs to field of feed additive technology, specifically a kind of novel liquid feed additive and preparation technology thereof.
Background technology
Along with China's entry into the WTO, in the face of the fierce international market unexpectedly striven, require that we must produce free of contamination green fish, shrimp, meat, egg, milk etc. and goods thereof, to guarantee that China's export product occupies share in international market, past is owing to studying at feed, trade management, the unsound factor of system of the aspects such as quality surveillance, harmful substance in dangerous feed product, environmental pollution is not caused by means of only animal excretion, can also be detained by the alimentary canal absorbing surface of animal, put aside in animal body, human body is entered by animal product, high risks is brought to health, as Belgium's " dioxin " pollutes meat, egg, milk event, " rabid ox disease " of Britain, China's Heyuan city hundreds of people is edible containing " clenbuterol hydrochloride " pork poisoning etc., all caused by dangerous feed, create significant impact at home and abroad.Visible, improve feed hygiene level of security, Promoting Standardized is produced, and promotes feed industry high technology industrialization, widelys popularize safe, efficient, free of contamination feed addictive imperative.
Summary of the invention
The present invention is directed to the problems referred to above; have developed a kind of novel liquid feed additive and preparation technology thereof; the feed addictive utilizing this manufacture craft to make to ecological environment not only without any destruction; and can effectively purify and protection of the environment; for numerous raisers provide free of contamination safe feed, and then ensure the life and health of the mankind.
Technical scheme of the present invention is:
One, a novel liquid feed additive, key is: this feed addictive is prepared from by the raw material of following parts by weight proportion relation:
Compound bio active bacteria formulation 10 ~ 12 parts,
Nutrient solution 18 ~ 20 parts,
Pure water 65 ~ 68 parts.
Wherein, described nutrient solution is prepared from by the component of following mass fraction proportion relation:
Glucose 35-40%,
Peptone 15-25%,
Trypsase 10-15%,
Surplus is soybean juice,
Wherein soybean juice is with soya bean: water=(70-75%): (25-30%) grinding forms.
Wherein, in the compound bio active bacteria formulation in this feed addictive, effective total viable count is no less than 10
8cfu/ml, said preparation activates in the test tube that supporting culture medium is housed by mixed bacteria, cultivates, generate in fermentation tank through shaking table, more after filtration, concentrated, proportioning forms active bacteria formulation, in said preparation, the percentage of the living bacteria count of contained various bacterial classification is:
Photosynthetic bacteria 22 ~ 25%,
Saccharomycete 21 ~ 24%,
Bacillus subtilis 15 ~ 20%
Pediococcus acidilactici 15 ~ 20%,
Streptococcus fecalis 12 ~ 16%,
Lactobacillus acidophilus 12 ~ 16%.
This compound bio active bacteria formulation can be produced by following preparation technology:
A, bacterial classification are chosen and activation:
In test tube, carry out inclined-plane according to selected species, the bacterial strain choosing health respectively and supporting activation medium and activate cultivation,
The determination of B, Shake flask medium and the cultivation of liquid spawn:
Required Shake flask medium is obtained than relation: multivitamin aqueous solution 2 parts, trace element water-soluble liquid 1 part, the activation culture based 1.5 part mixture supporting with bacterial classification shake up and disperses to inject flask by following volume parts, then add on the bacterial classification after activation, shaking table in 28-33 DEG C of desinfection chamber and cultivate 24-48 hour produce liquid bacterial classification
The proportioning of C, seeding tank culture medium and the fermentation of mixed bacteria are cultivated:
With reference to following component weight ratio configuration seeding tank culture medium: 35% dilute sulfuric acid 1ml, 7 water magnesium sulfate 2g, sodium chloride 1g, potassium dihydrogen phosphate 0.5g, natrium malicum 2g, 2 water calcium chloride 0.5g, yeast leachate 0.5g, 7 aqueous ferrous sulfate 0.1g, trace element water-soluble liquid 1ml, multivitamin aqueous solution 1ml, agar 10g, sucrose 5g, peptone 5g, add pure water 1L and form fluid nutrient medium
Inject aforesaid liquid culture medium in spawn fermentation tank after, in adjustment composite fluid bacterial classification, the effective total viable count of each bacterial classification reaches 10
6cfu/ml-10
7cfu/ml, and the mixed bacteria reaching following percentage composition with reference to each bacterial classification number of viable:
Photosynthetic bacteria 20 ~ 22%,
Saccharomycete 18 ~ 22%,
Bacillus subtilis 17 ~ 20%
Pediococcus acidilactici 18 ~ 21%,
Streptococcus fecalis 22 ~ 24%,
Lactobacillus acidophilus 22 ~ 18%,
Then join in fluid nutrient medium according to volume ratio 100:7 ~ 10 and stir, adjustment PH7.0, maintenance temperature 28-30 DEG C, incubation time obtains liquid spawn in 28 days;
D, secondary are cultivated:
With reference to following components by weight configuration secondary medium: agar 1kg, enzyme preparation 1kg, soybean juice 2.5kg, corn 40kg, wheat bran 8kg, dregs of beans 11kg, add pure water 876.5kg and form secondary medium, wherein, soybean juice is with soya bean: water=(70-75%): (25-30%) grinding forms;
Using the liquid spawn of step C gained as first class inoculum, the first class inoculum adding 60kg in secondary medium stirs, and adjustment PH7.0, keep temperature 30-33 DEG C, incubation time obtains required compound bio active bacteria formulation for 28 days.
Wherein, described trace element water-soluble liquid is mixed by following composition:
EDTA-2Na 190 ~ 210mg, 2 water sodium molybdate 18 ~ 22mg, 7 aqueous ferrous sulfate 80 ~ 120mg, 6 nickel oxide 8 ~ 12mg, boric acid 80 ~ 120mg, 2 water copper chloride 80 ~ 120mg, zinc chloride 80 ~ 120mg, 4 water manganese chloride 80 ~ 120mg, distilled water 0.8 ~ 1.2L.
Described multivitamin aqueous solution is mixed by following composition:
Vitamin B1: 480 ~ 520g, p-aminobenzoic acid: 280 ~ 320g, vitamin B3: 480 ~ 520mg, vitamin B12 40 ~ 60mg, vitamin B6: 80 ~ 120mg, VB7: 40 ~ 60mg, distilled water 0.8 ~ 1.2L.
Preferably, described trace element water-soluble liquid is mixed by following composition:
EDTA-2Na 200mg, 2 water sodium molybdate 20mg, 7 aqueous ferrous sulfate 100mg, 6 nickel oxide 10mg, boric acid 100mg, 2 water copper chloride 100mg, zinc chloride 100mg, 4 water manganese chloride 100mg, distilled water 1L.
Preferably, described multivitamin aqueous solution is mixed by following composition:
Vitamin B1: 500g, p-aminobenzoic acid: 300g, vitamin B3: 500mg, vitamin B12: 50mg, vitamin B6: 100 mg, VB7: 50mg, distilled water 1L.
Two, a preparation technology for novel liquid feed additive, key is: described processing step comprises:
A, take 10 ~ 12 weight portions the pure water of compound bio active bacteria formulation and 65 ~ 68 weight portions in storage tank, then the nutrient solution adding 18 ~ 20 weight portions carries out expansion cultivation 28 ~ 32 days, and it is 28 ~ 32 DEG C that temperature controls;
The qualified rear filling finished product of B, sample analysis chemical examination.
The qualified index of the sample analysis chemical examination of described step e is: bacillus subtilis every milliliter is not less than 2.1 × 10
8individual, Pediococcus acidilactici every milliliter is not less than 1.6 × 10
8individual, lactobacillus acidophilus every milliliter is not less than 10 × 10
8individual, streptococcus fecalis every milliliter is not less than 1.4 × 10
8individual, pH value 2.0 ~ 5.5.
The invention has the beneficial effects as follows: 1, feed addictive of the present invention is rich in multiple biologic live bacteria and high activity organic nutrient substance, adopt modern biotechnology, be composited, after feeding and drinking-water enters animal intestinal, strong dominant microflora can be formed with beneficial bacterium in enteron aisle, suppress and eliminate pathogenic bacteria, the a large amount of amino acid of secretion synthesis simultaneously, protein, nutriment needed for the animals such as vitamin, regulate and improve livestock and poultry body and each organ dysfunction, improve food conversion ratio, reduce or eliminate fecaluria foul smell, the Growth and reproduction of effective promotion animal, improve survival rate, purification human settlement, improve the economic and social benefits.2, utilize the crop wastes such as peanut skin, rice husk for raw material in the present invention, have the meaning of comprehensive utilization of resources and deep processing, energy saving is strong.3, raw materials usedly in the present invention to utilize completely, realize zero-emission, to ecological environment not only without any destruction, and can effectively purify and protection of the environment, for numerous raisers provide free of contamination safe feed.
Detailed description of the invention
Each probio in compound bio active bacteria formulation in the present invention all selects the bacterial classification preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center, wherein, photosynthetic bacteria (Inoculant of photosynthetic bacteria) be numbered 1.2352, saccharomycete (Saccharomyces sp) be numbered 20122, bacillus subtilis (Bacillus subtilissubsp.subtilis) be numbered 1.0015, Pediococcus acidilactici (Pediococcus acidilactici) be numbered 1.2696, streptococcus fecalis (Lactobacillus acidophilus) be numbered 10180, lactobacillus acidophilus (Lactobacillus acidophilus) be numbered 1.2686.
When buying collection bacterial classification, the planning standard activation medium model that bacterium Tibetan keeping unit can provide on the application direction of different strain is for reference, and the culture medium as activated yeast is YPD ... activation medium LB ... activation medium MRS.The above culture medium being matched in actication of culture is referred to as supporting culture medium.
A, bacterial classification are chosen and activation:
In test tube, carry out inclined-plane according to selected species, the bacterial strain choosing health respectively and supporting activation medium and activate cultivation,
The determination of B, Shake flask medium and the cultivation of liquid spawn:
Required Shake flask medium is obtained than relation: multivitamin aqueous solution 2 parts, trace element water-soluble liquid 1 part, the activation culture based 1.5 part mixture supporting with bacterial classification shake up and disperses to implant flask by following volume parts, then add on the bacterial classification after activation, shaking table in 28-33 DEG C of desinfection chamber and cultivate 24-48 hour produce liquid bacterial classification
The proportioning of C, seeding tank culture medium and the fermentation of mixed bacteria are cultivated:
With reference to following component weight ratio configuration seeding tank culture medium: 35% sulfuric acid 1g, 7 water magnesium sulfate 2g, sodium chloride 1g, potassium dihydrogen phosphate 0.5g, natrium malicum 2g, 2 water calcium chloride 0.5g, yeast leachate 0.5g, 7 aqueous ferrous sulfate 0.1g, trace element water-soluble liquid 1ml, multivitamin aqueous solution 1ml, agar 10g, sucrose 5g, peptone 5g, add the formation fluid nutrient medium that pure water forms 1L
Inject fluid nutrient medium in seeding tank after, the effective total viable count of each bacterial classification in composite fluid bacterial classification must be adjusted and reach 10
6cfu/ml-10
7cfu/ml, and carry out adjustment with reference to the following percentage composition of each bacterial classification number of viable and become mixed bacteria:
Photosynthetic bacteria 20 ~ 22%,
Saccharomycete 18 ~ 22%,
Bacillus subtilis 17 ~ 20%
Pediococcus acidilactici 18 ~ 21%,
Streptococcus fecalis 22 ~ 24%,
Lactobacillus acidophilus 22 ~ 18%,
Then stirring with long-pending to join in fluid nutrient medium than 100:7 ~ 10 according to expanding the fermentation tank size of cultivating, adjustment PH7.0, keeping temperature 28-30 DEG C, incubation time obtains liquid spawn in 28 days;
D, secondary are cultivated:
With reference to following components by weight configuration secondary medium: agar 1kg, enzyme preparation 1kg, soybean juice 2.5kg, corn 40kg, wheat bran 8kg, dregs of beans 11kg, add pure water 876.5kg and form secondary medium,
Using the liquid spawn of step C gained as first class inoculum, the first class inoculum adding 60kg in secondary medium stirs, adjustment PH7.0, keeps temperature 30-33 DEG C, and incubation time obtains preparing the compound bio active bacteria formulation needed for liquid feed additive of the present invention for 28 days.
Then, the pure water of the above-mentioned compound bio active bacteria formulation and 65 ~ 68 weight portions that take 10 ~ 12 weight portions is in storage tank, then the nutrient solution adding 18 ~ 20 weight portions carries out expansion cultivation 28 ~ 32 days, and it is 28 ~ 32 DEG C that temperature controls; The qualified rear filling finished product of last sample analysis chemical examination is liquid feed addictive.
Provide the embodiment of each raw material weight number proportion relation in this feed addictive below, refer to table 1:
Table 1
| Title | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| Compound bio live bacteria agent | 10 | 11 | 11 | 10 | 12 |
| Nutrient solution | 18 | 18 | 19 | 20 | 20 |
| Pure water | 66 | 67 | 65 | 68 | 67 |
The trace element water-soluble liquid that compound bio active bacteria formulation needed for preparation is used is the experience proportioning being adapted to each uniform growth demand in mixed bacteria and drawing through repetition test and formula rate adjustment.
Embodiment is as follows:
Embodiment (mg) 1234
EDTA-2Na 190 195 205 210
2 water sodium molybdates 18 19.5 20.5 22
7 aqueous ferrous sulfates 80 90 105 120
6 water nickel oxides 12 11 10 8
Boric acid 80 90 105 120
2 water copper chlorides 80 90 105 120
Zinc chloride 80 90 105 120
4 water manganese chlorides 80 90 105 120
Add distilled water 1 liter
Equally, preparing compound bio active bacteria formulation multivitamin aqueous solution used is also the experience proportioning being adapted to each uniform growth demand in mixed bacteria and drawing through repetition test and formula rate adjustment.Specific embodiment is as follows:
Embodiment is as follows:
Embodiment (g) A B C D
Vitamin B1 480 490 505 520
P-aminobenzoic acid 320 310 300 180
Cobastab
3480 490 505 520
Cobastab
1240 50 55 60
Cobastab
6120 110 95 80
Cobastab
40 48 52 60
Distilled water (L) 1.2 1.1 9.5 8
This feed addictive is approved through the feeding experiment of the raisers such as scale fish, jellyfish, shrimp, crab, pig, chicken, ox, fox, racoon dog, there has also been certain selling market at home.Has been issued by the ministry of agriculture of the People's Republic of China "microbial additive feed grade" and "additive premix feed" two production license, the product is the national brand, create a good social environment for the farmers and economic benefits.
Claims (10)
1. a novel liquid feed additive, is characterized in that: this feed addictive is prepared from by the raw material of following parts by weight proportion relation:
Compound bio active bacteria formulation 10 ~ 12 parts,
Nutrient solution 18 ~ 20 parts,
Pure water 65 ~ 68 parts.
2. a kind of novel liquid feed additive according to claim 1, is characterized in that: described nutrient solution is prepared from by the component of following weight percentage proportion relation:
Glucose 35-40%,
Peptone 15-25%,
Trypsase 10-15%,
Surplus is soybean juice,
Wherein soybean juice is with soya bean: water=(70-75%): (25-30%) grinding forms.
3. a kind of novel liquid feed additive according to claim 1, is characterized in that: in described compound bio active bacteria formulation, effective total viable count is no less than 10
8cfu/ml, said preparation activates in the test tube that supporting culture medium is housed by mixed bacteria, cultivates, generate in fermentation tank through shaking table, more after filtration, concentrated, proportioning forms active bacteria formulation, in said preparation, the percentage of the living bacteria count of contained various bacterial classification is:
Photosynthetic bacteria 22 ~ 25%,
Saccharomycete 21 ~ 24%,
Bacillus subtilis 15 ~ 20%,
Pediococcus acidilactici 15 ~ 20%,
Streptococcus fecalis 12 ~ 16%,
Lactobacillus acidophilus 12 ~ 16%.
4. a kind of novel liquid feed additive according to claim 1, is characterized in that: the step of preparation process of described compound bio active bacteria formulation comprises:
A, bacterial classification are chosen and activation:
In test tube, carry out inclined-plane according to selected species, the bacterial strain choosing health respectively and supporting activation medium and activate cultivation,
The determination of B, Shake flask medium and the cultivation of liquid spawn:
Required Shake flask medium is obtained than relation: multivitamin aqueous solution 2 parts, trace element water-soluble liquid 1 part, the activation culture based 1.5 part mixture supporting with bacterial classification shake up and disperses to inject flask by following volume parts, then add on the bacterial classification after activation, shaking table in 28-33 DEG C of desinfection chamber and cultivate 24-48 hour produce liquid bacterial classification
The proportioning of C, seeding tank culture medium and the fermentation of mixed bacteria are cultivated:
With reference to following component weight ratio configuration seeding tank culture medium: 35% dilute sulfuric acid 1ml, 7 water magnesium sulfate 2g, sodium chloride 1g, potassium dihydrogen phosphate 0.5g, natrium malicum 2g, 2 water calcium chloride 0.5g, yeast leachate 0.5g, 7 aqueous ferrous sulfate 0.1g, trace element water-soluble liquid 1ml, multivitamin aqueous solution 1ml, agar 10g, sucrose 5g, peptone 5g, add pure water 1L and form fluid nutrient medium
Inject aforesaid liquid culture medium in spawn fermentation tank after, in adjustment composite fluid bacterial classification, the effective total viable count of each bacterial classification reaches 10
6cfu/ml-10
7cfu/ml, and the mixed bacteria reaching following percentage composition with reference to each bacterial classification number of viable:
Photosynthetic bacteria 20 ~ 22%,
Saccharomycete 18 ~ 22%,
Bacillus subtilis 17 ~ 20%,
Pediococcus acidilactici 18 ~ 21%,
Streptococcus fecalis 22 ~ 24%,
Lactobacillus acidophilus 22 ~ 18%,
Then join in fluid nutrient medium according to volume ratio 100:7 ~ 10 and stir, adjustment PH7.0, keep temperature 28-30 DEG C, incubation time obtains liquid spawn in 28 days;
D, secondary are cultivated:
With reference to following components by weight configuration secondary medium: agar 1kg, enzyme preparation 1kg, soybean juice 2.5kg, corn 40kg, wheat bran 8kg, dregs of beans 11kg, add pure water 876.5kg and form secondary medium, wherein soybean juice is with soya bean: water=(70-75%): (25-30%) grinding forms;
Using the liquid spawn of step C gained as first class inoculum, the first class inoculum adding 60kg in secondary medium stirs, and adjustment PH7.0, keep temperature 30-33 DEG C, incubation time obtains required compound bio active bacteria formulation for 28 days.
5. a kind of novel liquid feed additive according to claim 4, is characterized in that: described trace element water-soluble liquid is mixed by following composition:
EDTA-2Na 190 ~ 210mg, 2 water sodium molybdate 18 ~ 22mg, 7 aqueous ferrous sulfate 80 ~ 120mg, 6 nickel oxide 8 ~ 12mg, boric acid 80 ~ 120mg, 2 water copper chloride 80 ~ 120mg, zinc chloride 80 ~ 120mg, 4 water manganese chloride 80 ~ 120mg, distilled water 0.8 ~ 1.2L.
6. a kind of novel liquid feed additive according to claim 4, is characterized in that: described multivitamin aqueous solution is mixed by following composition:
Vitamin B1: 480 ~ 520g, p-aminobenzoic acid: 280 ~ 320g, vitamin B3: 480 ~ 520mg, vitamin B12 40 ~ 60mg, vitamin B6: 80 ~ 120mg, VB7: 40 ~ 60mg, distilled water 0.8 ~ 1.2L.
7. a kind of novel liquid feed additive according to claim 4, is characterized in that: described trace element water-soluble liquid is mixed by following composition:
EDTA-2Na 200mg, 2 water sodium molybdate 20mg, 7 aqueous ferrous sulfate 100mg, 6 nickel oxide 10mg, boric acid 100mg, 2 water copper chloride 100mg, zinc chloride 100mg, 4 water manganese chloride 100mg, distilled water 1L.
8. a kind of novel liquid feed additive according to claim 4, is characterized in that: described multivitamin aqueous solution is mixed by following composition:
Vitamin B1: 500g, p-aminobenzoic acid: 300g, vitamin B3: 500mg, vitamin B12: 50mg, vitamin B6: 100 mg, VB7: 50mg, distilled water 1L.
9. the preparation technology of a kind of novel liquid feed additive as claimed in claim 1, is characterized in that: described processing step comprises:
A, take 10 ~ 12 weight portions the pure water of compound bio active bacteria formulation and 65 ~ 68 weight portions in storage tank, then the nutrient solution adding 18 ~ 20 weight portions carries out expansion cultivation 28 ~ 32 days, and it is 28 ~ 32 DEG C that temperature controls;
The qualified rear filling finished product of B, sample analysis chemical examination.
10. the preparation technology of a kind of novel liquid feed additive according to claim 9, is characterized in that: the qualified index of the sample analysis chemical examination of described step e is: bacillus subtilis every milliliter is not less than 2.1 × 10
8individual, Pediococcus acidilactici every milliliter is not less than 1.6 × 10
8individual, lactobacillus acidophilus every milliliter is not less than 10 × 10
8individual, streptococcus fecalis every milliliter is not less than 1.4 × 10
8individual, pH value 2.0 ~ 5.5.
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| CN106234268A (en) * | 2016-08-03 | 2016-12-21 | 大连海洋大学 | Rhopilema esculenta ecological pond culture nutritional solution and utilize the method that this nutritional solution carries out Rhopilema esculenta nursing |
| CN106472869A (en) * | 2015-08-23 | 2017-03-08 | 射阳盛海海蜇育苗有限公司 | A kind of nutritional solution improving Rhopilema esculenta shoot survival percent |
| CN107279465A (en) * | 2017-08-15 | 2017-10-24 | 赵福振 | Fish nutrition liquid and preparation method rich in biologically active nanometer organic zinc selenium |
| CN109316472A (en) * | 2018-11-29 | 2019-02-12 | 河南欧普生物科技有限公司 | A kind of preparation method of multivitamin oral solution for animals |
| CN114128808A (en) * | 2020-09-03 | 2022-03-04 | 河北科星药业有限公司 | Fish liquid feed additive capable of promoting digestion and preparation method and application thereof |
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Application publication date: 20150520 |