CN104585684A - Production method of pickles added with cordyceps sobolifera fungus - Google Patents
Production method of pickles added with cordyceps sobolifera fungus Download PDFInfo
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- CN104585684A CN104585684A CN201510031617.4A CN201510031617A CN104585684A CN 104585684 A CN104585684 A CN 104585684A CN 201510031617 A CN201510031617 A CN 201510031617A CN 104585684 A CN104585684 A CN 104585684A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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Abstract
The invention discloses a production method of pickles added with cordyceps sobolifera fungus. The method comprises the following steps: weighing white radish, fresh ginger and garlic, mixing evenly, packing into a pickle jar, and adding mixed bacteria powder which is 0.05%-0.2% of the vegetable raw materials in mass; covering the jar with a lid, and sealing with water to ensure an oxygen-free environment in the jar; fermenting at 20-30 DEG C for 5-8 hours; and adding a cordyceps sobolifera fungus fruiting body enzymolysis liquid, and further fermenting for 58-80 hours, wherein the mixed bacteria powder comprises the following components in parts by weight: 7-10 parts of lactobacillus plantarum and 1 part of bacillus aceticus.
Description
Technical field:
The present invention utilizes cicada fungus mushroom entity to reduce Nitrite in Pickles content and improves the method for pickle flavor, belongs to field of food.
Technical background:
Pickles are a kind of traditional foods of China, have the long history of more than 2000 year.It is mixed fermentation vegetables based on lactic acid bacteria in natural environment and flavoring, because local flavor is good to eat, be easy to store, nutrient health, therefore become a kind of popular food that Chinese generally like.
But the nitrite problem in pickles always is and receives much concern.Nitrite in pickles due in pickled and sweat nitrate reductase be that nitrite causes by the nitrate reduction in vegetables.After human body takes in nitrite, nitrogen-containing compound (secondary amine, tertiary amine, acid amides and amino acid) in nitrous acid and stomach is combined into the health of the direct harmful to human of the nitrosamine with carcinogenicity, people are quite scrupled for the consumption of pickles, and this becomes one of key factor of restriction pickles development.
Clear stipulaties in national standard (GB2762 ?2005), the content of catsup and pickled vegetables nitrite should more than 20mg/kg sample.In order to reduce the content of nitrite in catsup and pickled vegetables, Chinese scholars has carried out in many ways making great efforts.
At present, the method reducing Nitrite in Pickles mainly contains two classes: a class is by biodegradable method, namely the fermentation strain of the artificial method seed selection high degradability be separated is adopted, carry out method that is purebred or fermented by mixed bacterium, get rid of reproducibility as far as possible and to mix the increase of microbial content of nitrite.Another kind of is adopt the method for physics and chemistry, by the negative effect regulating the osmotic pressure of zymotic fluid or regulate the one-tenth of zymotic fluid to assign to suppress reproducibility microorganism to cause, as added material containing sulfydryl, hydroxyl to reduce the content of nitrite.
And under study for action, the discovery of our novelty adds cicada fungus mushroom entity can reduce content of nitrite in pickles, and successful.The present invention, by adding cicada fungus mushroom entity in the curing process of pickles, provides a kind of new technology and thinking for reducing Nitrite in Pickles content.
Summary of the invention
The invention provides a lactobacillus plantarum, it is strong and effectively can reduce the content of pickle production process nitrite that this bacterial strain produces high, the tolerance of acid amount.
Technical scheme of the present invention:
The invention provides a kind of pickle production method of adding cicada fungus bacterium; Comprise the steps:
Take ternip, ginger and garlic mixing and load pickle jar, add the Mixed Microbes powder of vegetable raw-material quality 0.05-0.2%, cover lid, use water seal mouth, guarantee the oxygen-free environment in altar, fermentation temperature is 20-30 DEG C, after fermentation 5-8h, add cicada fungus mushroom entity enzymolysis liquid, continue fermentation 58-80 hour.
Mixed Microbes powder parts by weight consist of: Lactobacillus plantarum 7-10, acetobacter 1.Acetobacter is the bacterium powder product of market sale or is prepared by common acetobacter fermented and cultured freeze drying.
Cicada fungus bacterium (Cordyceps sp.ACCC51169) fructification enzymolysis liquid: square cicada fungus mushroom entity being cut into 1 × 1cm, material-water ratio is 1:10, temperature 55-60 degree, pH6.5, add neutral cellulase (Ningxia jade of the He family Bioisystech Co., Ltd), neutral cellulase addition is the 0.1-1% of cicada fungus mushroom entity quality, and speed of agitator 50-60 turns/min, enzymolysis 3-5h.Then regulate temperature 40 degree, pH is 3.0, and add acid protease (Shandong Su Kehan bioengineering limited company), acid protease addition is the 0.1-1% of cicada fungus mushroom entity quality, and speed of agitator 50-60 turns/min, enzymolysis 2-4h.
Feedstock treating: picked by all pickles raw materials, peeling, stripping and slicing is for subsequent use.Clean all pickles raw materials with cold water, comprise, ternip, ginger, garlic etc., drain for subsequent use.
Every day, sampling detected ternip nitrite, found that, from second day, in whole sweat, ternip nitrite is all in below 2.7mg/kg, is starkly lower than the content of nitrite of ternip in traditional pickling process process, meets national standard.Fragrance is stronger, and brittleness is moderate.
Wherein, ternip 40-50: ginger: 0.5-1: cicada fungus mushroom entity: 5-8: garlic is: 3-10 (ratio of weight and number)
Described Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on July 2nd, 2014, preserving number is CGMCC NO.9405, preservation address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, Institute of Microorganism, Academia Sinica, postcode: 100101.
Described Lactobacillus plantarum tlj-2014 adopts following flow process to carry out seed selection:
The original bacterial classification that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → nitrosoguanidine (NTG) mutagenesis → plasma mutagenesis → dull and stereotyped primary dcreening operation → shaking flask sieves → mitotic stability test again.
Described Lactobacillus plantarum tlj-2014 has following character:
(1) production of lactic acid speed can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L;
(2) have acid resistance, when pH 1.80, survival is good;
(3) degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate.
Beneficial effect:
Through experiment, Lactobacillus plantarum tlj-2014 provided by the present invention finds that genetic stability is comparatively strong, inclined-plane can go down to posterity for continuous ten times, and proterties does not have significant change, property indices is all normal; Production of lactic acid speed can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L; Can survive under pH is the condition of 1.80, can resistance to 1% cholate; Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg, this bacterial classification when producing pickles, whole sweat nitrite concentration at below 5mg/kg, far below the content specified in standard GB/T 2714-2003 (20mg/kg).
In experimentation, unexpected discovery is added cicada fungus mushroom entity enzymolysis liquid and is effectively reduced content of nitrite after fermentation 5h, and in whole sweat, ternip nitrite is all in below 2.7mg/kg; The pickles content of nitrite not adding cicada fungus bacterium after fermentation 5-8h is in 4.5-5.0mg/kg.
Specific embodiment 1:
Feedstock treating: picked by all pickles raw materials, peeling, stripping and slicing is for subsequent use.Clean all pickles raw materials with cold water, comprise, ternip, ginger, garlic etc., drain for subsequent use.
Cicada fungus bacterium (Cordyceps sp.ACCC51169) fructification enzymolysis liquid: square cicada fungus mushroom entity being cut into 1 × 1cm, material-water ratio is 1:10, temperature 55 degree, pH6.5, add neutral cellulase (Ningxia jade of the He family Bioisystech Co., Ltd) 1000u/g cicada fungus mushroom entity, speed of agitator 300 turns/min, enzymolysis 3.5h.Then regulate temperature 40 degree, pH is 3.0, adds acid protease (Shandong Su Kehan bioengineering limited company) 500u/g cicada fungus mushroom entity, speed of agitator 300 turns/min, enzymolysis 3.5h.
The configuration of Mixed Microbes powder: Lactobacillus plantarum: acetobacter is 10:1 (weight ratio), bacterium powder addition is 0.05-0.2% (bacterium grain weight amount: vegetables weight); Described Lactobacillus plantarum is CGMCC9405, and acetobacter is CICC21684.
Pickle: take ternip 5kg, to scale (ternip 50: ginger 0.5: fructification 5: garlic 3, weight fraction ratio) and add ginger and garlic mixing loading pickle jar, add Mixed Microbes powder; Cover lid, water seal mouth, guarantees the oxygen-free environment in altar, and fermentation temperature is 25 DEG C, after fermentation 5h, adds cicada fungus mushroom entity enzymolysis liquid, continues fermentation to 60 hours.
Every day, sampling detected ternip nitrite, found that, from second day, in whole sweat, ternip nitrite is all in below 2.7mg/kg, is starkly lower than the content of nitrite of ternip in traditional pickling process process, meets national standard.
Embodiment 2 is basic with 1
Feedstock treating: picked by all pickles raw materials, peeling, stripping and slicing is for subsequent use.Clean all pickles raw materials with cold water, comprise, ternip, ginger, garlic etc., drain for subsequent use.
Cicada fungus bacterium (Cordyceps sp.ACCC51169) fructification enzymolysis liquid: square cicada fungus mushroom entity being cut into 1 × 1cm, material-water ratio is 1:10, temperature 55 degree, pH6.5, add neutral cellulase (Ningxia jade of the He family Bioisystech Co., Ltd) 1000u/g cicada fungus mushroom entity, speed of agitator 300 turns/min, enzymolysis 5h.Then regulate temperature 40 degree, pH is 3.0, adds acid protease (Shandong Su Kehan bioengineering limited company) 500u/g cicada fungus mushroom entity, speed of agitator 300 turns/min, enzymolysis 3h.
The configuration of Mixed Microbes powder: Lactobacillus plantarum: acetobacter is 7:1 (weight ratio), bacterium powder addition is 0.05% (bacterium grain weight amount: vegetables weight); Described Lactobacillus plantarum is CGMCC9405, and acetobacter is CICC21684.
Pickle: take ternip 5kg, to scale (ternip 50: ginger 0.5: fructification 5: garlic 3, weight fraction ratio) and add ginger and garlic mixing loading pickle jar, add Mixed Microbes powder; Cover lid, water seal mouth, guarantees the oxygen-free environment in altar, and fermentation temperature is 25 DEG C, after fermentation 8h, adds cicada fungus mushroom entity enzymolysis liquid, continues fermentation to 60 hours.
Every day, sampling detected ternip nitrite, found that, from second day, in whole sweat, ternip nitrite is all in below 2.7mg/kg, is starkly lower than the content of nitrite of ternip in traditional pickling process process, meets national standard.
Embodiment 3
Feedstock treating: picked by all pickles raw materials, peeling, stripping and slicing is for subsequent use.Clean all pickles raw materials with cold water, comprise, ternip, ginger, garlic etc., drain for subsequent use.
Cicada fungus bacterium (Cordyceps sp.ACCC51169) fructification enzymolysis liquid: square cicada fungus mushroom entity being cut into 1 × 1cm, material-water ratio is 1:10, temperature 55 degree, pH6.5, add neutral cellulase (Ningxia jade of the He family Bioisystech Co., Ltd) 1000u/g cicada fungus mushroom entity, speed of agitator 300 turns/min, enzymolysis 5h.Then regulate temperature 40 degree, pH is 3.0, adds acid protease (Shandong Su Kehan bioengineering limited company) 500u/g cicada fungus mushroom entity, speed of agitator 300 turns/min, enzymolysis 3h.
The configuration of Mixed Microbes powder: Lactobacillus plantarum: acetobacter is 8:1 (weight ratio), bacterium powder addition is 0.2% (bacterium grain weight amount: vegetables weight); Described Lactobacillus plantarum is CGMCC9405, and acetobacter is CICC21684.
Pickle: take ternip 5kg, to scale (ternip 50: ginger 0.5: fructification 5: garlic 3, weight fraction ratio) and add ginger and garlic mixing loading pickle jar, add Mixed Microbes powder; Cover lid, water seal mouth, guarantees the oxygen-free environment in altar, and fermentation temperature is 25 DEG C, after fermentation 6h, adds cicada fungus mushroom entity enzymolysis liquid, continues fermentation to 60 hours.
Every day, sampling detected ternip nitrite, found that, from second day, in whole sweat, ternip nitrite is all in below 2.7mg/kg, is starkly lower than the content of nitrite of ternip in traditional pickling process process, meets national standard.
In Tianjin according to Different age group (15-25 year, 26-35 year, 36-45 year and more than 46 years old) choose 50 people respectively and taste, and formulate grade form, statistics, be averaged a point statistics, as shown in the table.The product fragrance that the method is produced is stronger, and brittleness is moderate, and integrated sensory evaluates far above commercially available prod.
Grade form
Claims (8)
1. one kind is added the pickle production method of cicada fungus bacterium; Comprise the steps: that taking ternip, ginger and garlic mixing loads pickle jar, add the Mixed Microbes powder of vegetable raw-material quality 0.05-0.2%, cover lid, use water seal mouth, guarantee the oxygen-free environment in altar, fermentation temperature is 20-30 DEG C, after fermentation 5-8h, add cicada fungus mushroom entity enzymolysis liquid, continue fermentation 58-80 hour; Mixed Microbes powder parts by weight consist of: Lactobacillus plantarum 7-10, acetobacter 1.
2. add the pickle production method of cicada fungus bacterium according to claim 1, it is characterized in that, cicada fungus mushroom entity enzymolysis liquid is prepared as follows: fructification is cut into the square of 1 × 1cm, material-water ratio is 1:10, temperature 55-60 degree, pH6.5, add neutral cellulase, neutral cellulase addition is the 0.1-1% of cicada fungus mushroom entity quality, and speed of agitator 50-60 turns/min, enzymolysis 3-5h.Then regulate temperature 40 degree, pH is 3.0, adds acid protease, and acid protease addition is the 0.1-1% of cicada fungus mushroom entity quality, and speed of agitator 50-60 turns/min, enzymolysis 2-4h.
3., according to the pickle production method of adding cicada fungus bacterium according to claim 1, it is characterized in that, wherein, ternip 40-50: ginger: 0.5-1: cicada fungus mushroom entity: 5-8: garlic is: 3-10.
4. add the pickle production method of cicada fungus bacterium according to claim 1, it is characterized in that described Lactobacillus plantarum preserving number is CGMCC NO.9405.
5. according to claim 4 add the pickle production method of cicada fungus bacterium, it is characterized in that, described Lactobacillus plantarum has following character:
(1) production of lactic acid speed can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L;
(2) have acid resistance, when pH 1.80, survival is good;
(3) degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate.
6. add the pickle production method of cicada fungus bacterium according to claim 1, it is characterized in that, cicada fungus bacterium (Cordyceps sp.ACCC51169) fructification enzymolysis liquid: square cicada fungus mushroom entity being cut into 1 × 1cm, material-water ratio is 1:10, temperature 55 degree, pH6.5, adds neutral cellulase 1000u/g cicada fungus mushroom entity, speed of agitator 300 turns/min, enzymolysis 3.5h.Then regulate temperature 40 degree, pH is 3.0, adds acid protease 500u/g cicada fungus mushroom entity, speed of agitator 300 turns/min, enzymolysis 3.5h.
7. add the pickle production method of cicada fungus bacterium according to claim 1, it is characterized in that the configuration of Mixed Microbes powder: Lactobacillus plantarum: acetobacter is 10:1, bacterium powder addition is 0.05-0.2%; Described Lactobacillus plantarum is CGMCC9405, and acetobacter is CICC21684.
8. add the pickle production method of cicada fungus bacterium according to claim 1, it is characterized in that pickling: take ternip 5kg, to scale (ternip 50: ginger 0.5: fructification 5: garlic 3, weight fraction ratio) add ginger and garlic mixing loading pickle jar, add Mixed Microbes powder; Cover lid, water seal mouth, guarantees the oxygen-free environment in altar, and fermentation temperature is 25 DEG C, after fermentation 5h, adds cicada fungus mushroom entity enzymolysis liquid, continues fermentation to 60 hours.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419970A (en) * | 2018-01-29 | 2018-08-21 | 滁州学院 | A kind of chu chrysanthemum Periostracum cicadae fermented beverage and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102899262A (en) * | 2012-05-31 | 2013-01-30 | 浙江正味食品有限公司 | Lactobacillus plantarum and method for fast degrading nitrite produced in fermentation by the lactobacillus plantarum |
| CN103027290A (en) * | 2012-12-21 | 2013-04-10 | 中国农业大学 | Application of boletus edulis in reducing nitrite content in fermented food or pickled food |
Non-Patent Citations (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419970A (en) * | 2018-01-29 | 2018-08-21 | 滁州学院 | A kind of chu chrysanthemum Periostracum cicadae fermented beverage and preparation method thereof |
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Application publication date: 20150506 |