CN104509631B - Full coffee peptide nutrient food and preparation method thereof - Google Patents
Full coffee peptide nutrient food and preparation method thereof Download PDFInfo
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- CN104509631B CN104509631B CN201410848904.XA CN201410848904A CN104509631B CN 104509631 B CN104509631 B CN 104509631B CN 201410848904 A CN201410848904 A CN 201410848904A CN 104509631 B CN104509631 B CN 104509631B
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Abstract
The present invention is a kind of to be aided with full coffee peptide nutrient food of soybean and preparation method thereof by major ingredient of coffee bean, the present invention full coffee peptide nutrient food contain 1~60% weight than the coffee peptide for absorption of human body, also containing linoleic acid, caffeine, bifidus factor, saponin, VB, calcium, iron, phosphorus, selenium and glucose etc..It is that add water grinding slurrying after crushing first by coffee bean in 80~150 DEG C of bakings；Again by the grinding slurrying that added water after soybeans soaking；Coffee slurries are mixed after 40~60Mpa high-pressure homogeneous 2~4 times with big soybean milk liquid, then grinding subdivision, heating under 135~160 DEG C and 7~8kg pressure, then add lipase and cellulase standing is biochemical, adding bromelain, constant temperature is biochemical again；Slurries after biochemistry are gone out after enzyme sterilizing, system is separated out by the sheet frame of the filter aid containing food grade kieselguhr and separated out, full coffee peptide stoste is produced, and then full coffee peptide nutrient food is made.
Description
Technical field
The present invention is that one kind uses biotechnology and modern physics nanometer technology principle using coffee bean to be main former
Material is aided with full coffee peptide nutrient food of Soybean production and preparation method thereof.
Background technology
The nutritional ingredient that coffee contains is very abundant：2.2 grams of moisture content, 12.6 grams of protein, fat in every 100 grams of coffee beans
16 grams, 46.7 grams of carbohydrate, 9 grams of cellulose, 4.2 grams of ash content, 120 milligrams of calcium, 170 milligrams of phosphorus, 42 milligrams of iron, 3 milligrams of sodium, dimension life
Plain B20.12 grams, 3.5 milligrams of nicotinic acid, 1.3 grams of caffeine, 8 grams of tannin.And every 99.5 grams of 100 grams of coffee leachates moisture content, egg
0.2 gram of white matter, 0.1 gram of fat, 0.1 gram of ash content, micro carbohydrate, 3 milligrams of calcium, 4 milligrams of phosphorus, 2 milligrams of sodium, the milli of vitamin B2 0.01
Gram, 0.3 milligram of nicotinic acid.10 grams of coffees are dissolved in hot water, content of caffeine be 0.04 gram, tannin content be 0.06 gram.Coffee
Because being most to behave in all compositions of coffee to gaze at, it can accelerate the metabolism of human body, make one to keep clearheaded and think
Dimension is sensitive；Fat：The fat that coffee is included, accounts for particularly important role on local flavor, and volatile fat is that coffee aroma is main
Source；Because protein is difficult dissolution in coffee, it is impossible to which be absorbed by the body utilization, and people can only have a taste of when drinking coffee pastime
Its delicious food and stimulate or even refresh oneself, and the complete nutrients matter of coffee can not be absorbed.
The content of the invention
Problem to be solved by this invention is to provide one kind by primary raw material of coffee bean through overbaking or through overbaking
It is aided with a small amount of soybean again afterwards, with receiving in the enzyme engineering in biotechnology, histone engineering and modern physics
Coffee protein, can be converted into the peptide that people's physical efficiency directly absorbs by full coffee peptide nutrient food prepared by meter Yuan Li and preparation method thereof
Etc. nutritional ingredient.Described peptide is the mixture of the compounds such as oligopeptide, small peptide and polypeptide.
Need to solve following difficult scientific problems to achieve the above object：One is to solve protein in coffee to be difficult to asking for dissolution
Topic；Two be to solve coffee protein to be difficult to be digested the disconnected and stubborn problem of protein chain；Three be to solve usual protein drop
Xie Yi is converted into a large amount of amino acid and is condensed into the low problem of the conversion ratio for being converted into peptide of a small amount of peptide；Four be to solve to drink coffee
The problem of coffee easily causes the three big side effects such as painstaking effort brain pipe disease, induced osteoporosis and carcinogenic danger.
The peptide that the full coffee peptide nutrient food that the present invention is provided contains 1~60% percentage by weight, also containing linoleic acid, flax
Acid, oleic acid, caffeine, bifidus factor, saponin, Flavonoid substances, phosphatide, VB, calcium, iron, phosphorus, selenium and glucose, also contain one
Quantitative water；Described full coffee peptide nutrient food is made by the following method：Coffee bean and the ratio of weight and number of soybean are 100
~60: 0~40；First coffee bean is toasted 30~50 minutes at 80~150 DEG C, it is broken after add water grinding slurrying, fineness up to 260~
500 mesh；Again by the grinding slurrying that added water after soybeans soaking, fineness is up to 200~300 mesh；After coffee slurries are mixed with big soybean milk liquid
High-pressure homogeneous 2~4 times, homogenization pressure is 40~60Mpa；Slurries after homogeneous are pressed in 135~160 DEG C of temperature and 7~8kg
Subdivision is ground under power, and is heated 35~40 minutes；Slurries are cooled to 30~55 DEG C, lipase is added and cellulase stands biochemistry
1.5~2.5 hours, then temperature is adjusted to 45~50 DEG C, add bromelain 4~8 hours of constant temperature biochemistry again；Will be raw
Slurries after change are warming up to 80~100 DEG C of enzyme sterilizings of going out, finally under conditions of temperature is 68~85 DEG C, by silicon containing food-grade
The sheet frame of diatomaceous earth filter aid separates out system and separated out, and produces full coffee peptide stoste, and then full coffee peptide nutrient food is made.
The present invention full coffee peptide nutrient food can be：It is full coffee Gly-His-Lys, full coffee peptide capsule, full coffee peptide tablet, complete
Coffee peptide oral liquid or the high peptide activity motion drink of full coffee.60% is may be up to for complete its peptide content of coffee Gly-His-Lys, and full coffee
The peptide content of peptide oral liquid or active motor beverage is more than 4%.
The preparation method of full coffee peptide nutrient food its step that the present invention is provided is as follows：
1) dispensing：Select fresh, free from admixture, without the coffee bean that goes mouldy and soybean, the ratio of weight and number of coffee bean and soybean is
100~60: 0~40；
2) coffee slurries are prepared
2.1) first coffee bean is toasted 30~50 minutes at 80~150 DEG C, is dark brown and aromatic flavour to color, makes it
Albuminous degeneration；
2.2) coffee bean after baking is crushed, then add water grinding 3~5 times, and fineness obtains coffee paste up to 260~500 mesh
Liquid, makes protein denaturation, more degradable and condensation；
3) big soybean milk liquid is prepared
3.1) soak：Soybean is soaked 8~10 hours in water temperature is 20~35 DEG C of water；
3.2) grinding subdivision：Soaked soybean is cleaned up with water and (generally cleaned 3~5 times), and add water defibrination, slurry
Big soybean milk liquid is obtained after slag separation, then big soybean milk liquid is at least ground 2 times in colloid mill, fineness makes albumen up to 200~300 mesh
Qualitative change, more degradable and condensation；
4) mix：Stirred after coffee slurries are mixed with big soybean milk liquid, mixing speed is 2700~3000 revs/min；
5) homogeneous：Homogeneous 2~4 times in high pressure homogenizer by mixed serum, homogenization pressure is 40~60Mpa, makes the two
Protein dispersibility blend evenly, be conducive to overcoming coffee easily to cause painstaking effort brain pipe disease, lure while biochemical degradation
Send out the big side effect of osteoporosis and carcinogenic danger etc. three；
6) evil is gone in deodorant：Slurries after homogeneous are ground into subdivision at 135~160 DEG C of temperature and 7~8kg pressure, and
Heating 35~40 minutes, fineness is 300-350 mesh, harmful substance in coffee and big soybean milk liquid is passivated, thoroughly overcomes and
Coffee easily induce cardio-cerebrovascular diseases and soybean intrinsic anti-trophism, cause Thyroid Gland Swell, digestive discomfort nausea, flatulence
With the five big side effects such as the easy gout of beans raw meat bitter taste and coffee and soybean protein is further denatured；
7) it is biochemical：Above-mentioned slurries are cooled to 30~55 DEG C, lipase is added and cellulase stirs, are stood biochemical
1.5~2.5 hours, then temperature is adjusted to 45~50 DEG C, add bromelain and stir, enzyme preparation is filled with slurries
Divide mixing, then 4~8 hours of constant temperature biochemistry；
8) go out enzyme, sterilizing：Slurries after biochemistry are warming up to 80~100 DEG C, gone out 15~30 seconds enzyme time；
9) separate out：By the material after the enzyme that goes out, sterilizing under conditions of temperature is 68~85 DEG C, by containing food grade kieselguhr
The sheet frame of filter aid separates out system and separated out, and produces full coffee peptide stoste, and then full coffee peptide nutrient food is made.
The amount containing peptide of above-mentioned full coffee peptide stoste is 0.8~1.5% (percentage by weight) left and right, can be passed through according to the actual requirements
Multistage concentration systems do multistage concentration, and peptide content can be made to reach more than 4%, then handled through multistage filtering, pass through vacuum spraying
Drying system, can draw full coffee Gly-His-Lys or full coffee peptide capsule or tablet are made again, or full coffee is made by aseptic subpackaged
Peptide oral liquid or the high peptide activity sports drink of full coffee.
Above-mentioned steps 1) in coffee bean and the ratio of weight and number of soybean be 100~60: 0.5~40；
Above-mentioned steps 2.2) in the ratio of weight and number of coffee bean and water be preferably 1: 6~8；Step 3.2) in soybean it is (dry big
Beans) with the ratio of weight and number of water it is preferably 1: 6~8.
Step 7) preferred scheme be：Above-mentioned slurries are cooled to 30~35 DEG C, addition account for slurry weight 0.03~
0.05% lipase and 0.01~0.025% cellulase stir, and stand biochemistry 1.5~2 hours, then adjust temperature
Whole to 45~50 DEG C, the bromelain that addition accounts for slurry weight 0.1~0.5% stirs, and makes enzyme preparation abundant with slurries
Mixing, then 6~8 hours of constant temperature biochemistry.
Step 9) in separate out effect preferred plan be：Coated in the sheet frame precipitation system of the filter aid containing food grade kieselguhr
The weight of food grade kieselguhr filter aid on sheet frame is calculated as 0.5~0.8 kilogram/square metre by sheet frame screen area.
The present invention is not only thoroughly overcome using implementation of the biotechnology by unique technique and crucial actual parameter
3 intrinsic big side effects of coffee, remain the original trophic function of coffee, have also obtained rich in for human body directly, fully absorb
Peptide, and hyperosmosis will not be caused to organs such as human body liver,kidney,spleen, brains, with direct enhancing human life vigor and exempted from
Epidemic disease function and inoxidizability, remove free radical, norcholesterol, anallergic promote lipid metaboli, reducing blood lipid, hypotensive, anti-inflammatory,
The special dietary healthcare functions such as beauty, antifatigue, anti-aging, anticancer, the infringement of suppression harmful bacteria and promotion growth of probiotics breeding,
This problem that coffee albumen for a long time is difficult to be absorbed by the body is solved, coffee protein once-used degradeable is condensed into peptide
High conversion rate up to more than 80%.
Embodiment
Present disclosure is described in detail with reference to specific embodiment.
1) choose 70kg without going mouldy, free from admixture, fresh coffee bean and 30kg soybean；
2) 70kg coffee bean is put into the oven for baking 50 minutes that temperature is 80 DEG C；
3) by coffee bean crusher in crushing, pure water 280kg is then added, is ground 3 times, fineness is obtained up to 260 mesh
Coffee slurries；
4) 30kg soybean is placed in the water that water temperature is 20 DEG C and soaked 8 hours；
5) soaked soybean is cleaned 3 times with pure water, then adds pure water 180kg and carry out screenings separating treatment,
Slurries after separation are ground 2 times in colloid mill, and fineness obtains big soybean milk liquid up to 200 mesh；
6) coffee slurries are mixed with big soybean milk liquid, is sufficiently stirred in 2700 revs/min of agitator tank, makes two
Person is well mixed；
7) above-mentioned mixed serum is entered into homogeneous 2 times in high pressure homogenizer, homogenization pressure is 400kg；
8) slurries after homogeneous are handled 35 minutes at 135 DEG C of temperature and 7 atmospheric pressures, fineness of grind is 300
Mesh；
9) above-mentioned slurries are cooled to 30 DEG C by the pipe heat exchanger 10 second time of work；Add and account in slurries after the cooling period
The lipase of slurry weight 0.03% and 0.01% cellulase stir, and stand biochemistry 1.5 hours, then adjust temperature
To 45 DEG C, 0.1% bromelain is added, by high-speed stirred, enzyme preparation is sufficiently mixed with slurries, keep constant temperature biochemical
8 hours；
10) material is warming up to 100 DEG C of enzymes that go out, sterilizing by pipe heat exchanger by the slurries after biochemistry, gone out the enzyme time 15
Second；
11) (contained by having precoated full coffee peptide stoste 450kg of the diatomaceous sheet frame precipitation system precipitation of food-grade or so
Peptide amount 0.8~1.5%), it is 0.5%kg/ squares that wherein the precoating amount of food grade kieselguhr filter aid is calculated by sheet frame screen area
Rice, sheet frame filter screen fineness is 600~3000 mesh, and the sheet frame filter screen fineness of this example is 1000 mesh；
12) the peptide liquid of above-mentioned precipitation is full coffee peptide stoste, and the full coffee peptide that multistage is concentrated to give more than 4% is carried out according to need
Dietary liquid 200kg or so, now, then aseptic subpackaged after multistage filtering and sterilizing is made full coffee peptide oral liquid or complete
The high peptide activity motion drink of coffee；Or the full coffee Gly-His-Lys of 20kg are made by vacuum spray drying system or full coffee peptide glue is made again
Capsule or tablet.
Claims (6)
1. a kind of full coffee peptide nutrient food, it is characterized in that containing 1~60% weight than coffee peptide, also containing linoleic acid, flax
Acid, oleic acid, caffeine, bifidus factor, saponin, Flavonoid substances, phosphatide, VB, calcium, iron, phosphorus, selenium and glucose, also contain one
Quantitative water；Described full coffee peptide nutrient food is made by the following method：Coffee bean and the ratio of weight and number of soybean are 100
~60: 0~40；First coffee bean is toasted 30~50 minutes at 80~150 DEG C, it is broken after add water grinding slurrying, fineness up to 260~
500 mesh；Again by the grinding slurrying that added water after soybeans soaking, fineness is up to 200~300 mesh；After coffee slurries are mixed with big soybean milk liquid
Stir, mixing speed is 2700~3000 revs/min；Then high-pressure homogeneous 2~4 times, homogenization pressure is 40Mpa；By homogeneous
Slurries afterwards grind subdivision at 135~160 DEG C of temperature and 7~8kg pressure, and heat 35~40 minutes；Slurries are cooled down
To 30~55 DEG C, add lipase and cellulase stands biochemistry 1.5~2.5 hours, then adjust temperature to 45~50 DEG C, plus
Enter bromelain 4~8 hours of constant temperature biochemistry again；Slurries after biochemistry are warming up to 80~100 DEG C of enzyme sterilizings of going out, finally existed
Temperature is under conditions of 68~85 DEG C, to separate out system by the sheet frame of the filter aid containing food grade kieselguhr and separate out, produce full coffee
Peptide stoste, and then full coffee peptide nutrient food is made.
2. full coffee peptide nutrient food according to claim 1, it is characterized in that full coffee peptide nutrient food is：Full coffee Gly-His-Lys,
Full coffee peptide capsule, full coffee peptide tablet, full coffee peptide oral liquid or the high peptide activity sports drink of full coffee.
3. the preparation method of full coffee peptide nutrient food described in claim 1, it is characterized in that step is as follows：
1) dispensing：Coffee bean and the ratio of weight and number of soybean are 100~60: 0~40；
2) coffee slurries are prepared
2.1) first coffee bean is toasted 30~50 minutes at 80~150 DEG C；
2.2) coffee bean after baking is crushed, then add water grinding 3~5 times, fineness obtains coffee slurries up to 260~500 mesh；
3) big soybean milk liquid is prepared
3.1) soak：Soybean is soaked 8~10 hours in water temperature is 20~35 DEG C of water；
3.2) grinding subdivision：Soaked soybean is cleaned up with water, and add water defibrination, and big soybean milk liquid is obtained after screenings separation, then will
Big soybean milk liquid is at least ground 2 times in colloid mill, and fineness is up to 200~300 mesh；
4) mix：Stirred after coffee slurries are mixed with big soybean milk liquid, mixing speed is 2700~3000 revs/min；
5) homogeneous：Homogeneous 2~4 times in high pressure homogenizer by mixed serum, homogenization pressure is 40Mpa；
6) evil is gone in deodorant：Slurries after homogeneous are ground into subdivision at 135~160 DEG C of temperature and 7~8kg pressure, and heated
35~40 minutes, fineness was up to 300~350 mesh；
7) it is biochemical：Above-mentioned slurries are cooled to 30~55 DEG C, lipase is added and cellulase stirs, standing biochemistry 1.5~
2.5 hours, then temperature is adjusted to 45~50 DEG C, add bromelain and stir, enzyme preparation is fully mixed with slurries
Close, then 4~8 hours of constant temperature biochemistry；
8) go out enzyme, sterilizing：Slurries after biochemistry are warming up to 80~100 DEG C, gone out 15~30 seconds enzyme time；9) separate out：To go out enzyme,
Material after sterilizing is under conditions of temperature is 68~85 DEG C, and the sheet frame for passing through the filter aid containing food grade kieselguhr separates out system analysis
Go out, produce full coffee peptide stoste, and then full coffee peptide nutrient food is made.
4. preparation method according to claim 3, it is characterized in that：
Step 2.2) in coffee bean and water ratio of weight and number be 1: 6~8；
Step 3.2) in soybean and water ratio of weight and number be 1: 6~8.
5. the preparation method according to claim 3 or 4, it is characterized in that：
Step 7) above-mentioned slurries are cooled to 30~35 DEG C, add the lipase for accounting for slurry weight 0.03% and 0.01~
0.025% cellulase stirs, and stands biochemistry 1.5 hours, then adjusts temperature to 45 DEG C, and addition accounts for slurry weight
0.1~0.5% bromelain stirs, and enzyme preparation is sufficiently mixed with slurries, then constant temperature biochemical 8 hours.
6. the preparation method according to claim 3 or 4, it is characterized in that：
Step 9) in the filter aid containing food grade kieselguhr sheet frame precipitation system in the food grade kieselguhr that is coated on sheet frame help
The weight of filtering agent is calculated as 0.5~0.8 kilogram/square metre by sheet frame screen area.
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| CN105368642A (en) * | 2015-12-15 | 2016-03-02 | 贵州大学 | Production method for preparing coffee compound wine with assistance of enzyme method |
| CN109007191B (en) * | 2018-09-06 | 2021-11-19 | 张家界金驰天问农业科技有限公司 | Giant salamander active peptide coffee |
| CN111248327B (en) * | 2020-01-19 | 2022-09-20 | 北京工商大学 | Processing method for improving quality of coffee beans by using soybean extract and coffee beans |
| CN113080288A (en) * | 2021-03-28 | 2021-07-09 | 广东亿盈新材料科技有限公司 | Method for preparing small molecular peptide coffee |
| CN114517219A (en) * | 2021-12-17 | 2022-05-20 | 云南文山坤七药业股份有限公司 | Preparation method and application of soybean, coffee and gastrodia elata bioactive peptide |
| CN114480536A (en) * | 2021-12-17 | 2022-05-13 | 云南文山坤七药业股份有限公司 | Preparation method and application of soybean, coffee and sealwort bioactive peptide |
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| US4983408A (en) * | 1988-12-07 | 1991-01-08 | Colton Ralph L | Method for producing coffee extracts |
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