CN104502170A - Immobilization device and immobilization method for pathological tissues - Google Patents

Abstract

The invention relates to an immobilization device and an immobilization method for pathological tissues. The immobilization device comprises a box body and a box cover assembly, wherein the box body is used for containing a immobilization solution and the pathological tissues; partition components for partitioning the box body into a plurality of chambers are arranged in the middle of the box body at intervals; and the box cover assembly is arranged at an upper end opening of the box body, and is used for sealing the box body. The immobilization device has the advantage of good immobilization effect on the pathological tissues; the cross contamination among the pathological tissues is avoided; and the concentration, the temperature and the pH value of the solution in the immobilization device can be detected in real time.

Description

A fixation device for pathological tissue and its fixation method

technical field

The invention relates to the technical field of medical instruments, in particular to a fixing device for pathological tissues and a fixing method thereof.

Background technique

In the medical field, in order to study the lesions and the extent of lesions in human organs (such as the stomach, lungs, or breasts, etc.), it is necessary to remove certain sized diseased tissues from the organs that have already undergone lesions. The excised diseased tissue is processed by various chemicals or embedding methods, so that the excised diseased tissue is fixed and hardened. The lesion tissue is cut into thin slices on a microtome, and the thin slices are adhered to a glass slide to achieve position fixation of the thin slices. At the same time, the thin slices are dyed to facilitate the observation of the morphology of pathological tissues under a microscope, so as to make accurate pathological judgments. Therefore, effective fixation of pathological tissues is a key step to ensure accurate pathological diagnosis.

Currently, the devices for fixing pathological tissues are usually soft bags. The soft bag is equipped with a fixing solution, and the fixing solution is used to soak the pathological tissue, so as to realize the fixing of the pathological tissue. However, the soft bag cannot separate each pathological tissue well, so there is a problem of cross-contamination between pathological tissues.

Contents of the invention

In view of the above problems, according to the first subject of the present invention, a fixation device for pathological tissue is proposed, which includes: a box for containing fixation solution and pathological tissue, and the box is provided with a The box body is divided into a partition part of a plurality of chambers; the box cover assembly is arranged at the upper port position of the box body, and the box cover assembly is used to seal the box body. The fixing device can properly adjust the size of the chamber in the box according to the different types and sizes of pathological tissues to be fixed, avoiding the limitation on the size and type of pathological tissues. In addition, since the box body is provided with a plurality of partition parts, the box body is divided into chambers independent of each other, so that not only can a plurality of pathological tissues be fixed at the same time, but also avoid the The disadvantages of cross-contamination of the pathological tissue, so that the fixation effect of the pathological tissue is better.

Preferably, the partition member is connected to the box in a plug-in type. In this way, the partition member can be reasonably arranged in the box body according to the different types and sizes of pathological tissues, so as to divide the box body into chambers of different sizes. The restriction on the type and size of pathological tissue is avoided, and pathological tissue of different types and sizes can be fixed. In addition, the partition part and the box body are connected in a plug-in type, which makes the installation of the partition part more flexible and facilitates the installation and disassembly of the partition part.

Preferably, the partition members are arranged at equal intervals in the box body. In this way, multiple pathological tissues of the same type can be fixed at one time, realizing the purpose of fixing pathological tissues in large quantities.

Preferably, a liquid level display component capable of displaying the liquid level of the fixing solution is provided on the outer wall of each chamber. The liquid level display part can display the liquid level height of the fixed solution in the corresponding chamber at all times, so that it is convenient for the operator to adjust the liquid level of the fixed solution in the chamber in time. The disadvantage of overflowing the box due to the high liquid level in the chamber is avoided, and the disadvantage of poor fixation effect on pathological tissues due to the low liquid level is also avoided.

Preferably, an ultraviolet sterilizing component for sterilizing pathological tissues in the chamber is fixedly installed on the inner wall of each chamber. The ultraviolet disinfection component can emit ultraviolet light, which has strong penetrating power and can irradiate the bacteria or viruses in the fixed solution, so that the bacteria or viruses cannot reproduce due to denaturation, so as to sterilize the pathological tissue and disinfection purposes. In this way, the cleanliness of the pathological tissue is ensured, and the disadvantage of the pathological tissue being rotted due to the propagation of its own virus or bacteria is avoided.

Preferably, a liquid discharge port is opened on the side wall of each chamber, and a filter net for filtering the pathological tissue is fixedly installed on the peripheral wall of the liquid discharge port. In this way, after the fixation of the pathological tissue is completed, the fixation solution soaked in the pathological tissue needs to be poured out. Therefore, through the setting of the liquid discharge port, the disadvantage of turning the entire fixation device over and pouring out the fixation solution is greatly improved. Reduce the labor intensity of the operator.

Preferably, the fixing device further includes a sealing cover that cooperates with the liquid discharge port and is used to seal the liquid discharge port. The sealing cover cooperates with the liquid discharge port, so as to realize the sealing effect on the liquid discharge port, and avoid the disadvantage that the fixing solution is discharged from the liquid discharge port during the process of fixing the pathological tissue.

Preferably, the side wall of each chamber is also provided with: a temperature detection component for detecting the temperature of the fixation solution; a concentration detection component for detecting the concentration of the fixation solution; A pH detection part for the pH of a solution. The temperature detection component can display the temperature of the fixation solution in the corresponding chamber at all times. Therefore, it is convenient for the operator to control the temperature of the fixation solution in time, avoiding the disadvantages of accelerating the volatilization speed of the fixation solution due to the high temperature, and avoiding the deterioration of the pathological tissue due to the high temperature of the fixation solution. At the same time, it is avoided that the fixation effect of the pathological tissue is affected due to the temperature of the fixation solution being too low. The concentration detection part can display the concentration of the fixation solution in the corresponding chamber at all times. Therefore, it is convenient for the operator to control the concentration of the fixing solution in time. The pH detection component can display the pH of the fixed solution in the corresponding chamber at all times. Therefore, it is convenient for the operator to control the pH of the fixing solution in time.

Preferably, an installation opening is opened on the cover assembly at a position corresponding to each of the chambers.

Preferably, the fixing device further includes a sealing box cover installed in the installation opening, and the sealing box cover is hinged with the box cover assembly. In this way, it is convenient for the operator to put or take out the pathological tissue into the corresponding chamber. At the same time, during the process of fixing the pathological tissue, the lid of the sealed box can be closed, so that the impurities outside the box can be effectively prevented from entering. into the fixation solution in the corresponding chamber, thereby affecting the fixation effect of pathological tissues.

Preferably, a liquid injection port is opened on each of the sealed box covers.

According to the second subject of the present invention, a method for fixing pathological tissues with a fixing device is proposed, the method includes: preparing a fixation solution with a preset concentration value; after the fixation solution is prepared, inject it into the corresponding chamber of the box body ;Detect whether the configured fixed solution reaches the preset concentration value; if it does not reach the preset concentration value, adjust the concentration of the fixed solution to reach the preset concentration value; if it has reached the preset concentration value, inject The pathological tissue to be fixed is put into the chamber of the fixing solution; the preset time required for fixing the pathological tissue is set, and the fixing of the pathological tissue is started; when the preset time is reached, it indicates that the fixing of the pathological tissue is completed.

According to the present invention, the fixation device has the advantages of avoiding cross-contamination between pathological tissues, having a good fixation effect on pathological tissues, and making the same batch of pathological tissues have the same fixation effect, and also has the advantages of being able to detect in real time the Advantages of solution concentration, temperature, and pH.

Description of drawings

Hereinafter, the present invention will be described in more detail based on the embodiments with reference to the accompanying drawings. In the picture:

Fig. 1 is a schematic diagram of the overall exploded structure of the fixation device for pathological tissue of the present invention.

Fig. 2 is a structural schematic diagram of a box body of the fixation device for pathological tissue according to the present invention.

Fig. 3 is a schematic structural view of the box cover assembly of the fixation device for pathological tissue according to the present invention.

Fig. 4 is a schematic flowchart of a method for fixing pathological tissues by the fixing device of the present invention.

In the figures, the same parts are given the same reference numerals. The drawings are not drawn to true scale.

Detailed ways

The present invention will be further described below in conjunction with accompanying drawing.

Please refer to FIG. 1 , which is a schematic diagram of the general exploded structure of the fixation device for pathological tissues of the present invention. As shown in the figure, the fixing device includes a box body 1 . The box body 1 is used to contain fixative solution and pathological tissue.

In the embodiment of the present application, partition members 11 are arranged at intervals in the box body 1 . The partition member 11 divides the box body 1 into a plurality of chambers 12, and each chamber 12 can be filled with fixative solution and pathological tissue. In this way, due to the setting of the partition member 11, the box body 1 is divided into a plurality of chambers 12 independent of each other, so that the purpose of simultaneously fixing multiple pathological tissues can be achieved, and it is also avoided to be located in adjacent chambers. The problem of cross-contamination between pathological tissues in 12. The immersion of the pathological tissue in the fixative solution causes the amino group on the protein in the pathological tissue to react with the formaldehyde molecule in the fixative solution, thereby making the protein cross-linked and denatured. Therefore, the degree of deformation of the pathological tissue can be reduced as much as possible, and the original shape of the pathological tissue when it is cut from the diseased organ can be better maintained, so that the fixation effect of the pathological tissue is better. In the embodiment of the present application, the specific design number of the chamber 12 can be determined according to the specific number of pathological tissues to be fixed at that time.

In the embodiment of the present application, the fixing solution may be a neutral buffered formalin solution. The penetration of the neutral buffered formalin solution is strong, so that the shrinkage of the pathological tissue is small, thereby better maintaining the original shape of the pathological tissue when it is just cut from the diseased organ, so that the fixation effect of the pathological tissue can be improved. better.

In addition, the concentration range of the neutral buffered formalin solution is 9.5% to 10%, so that the fixing effect on pathological tissues is better. Since the neutral buffered formalin solution is neutral, it is beneficial to the fixation of the pathological tissue and avoids affecting the fixation effect of the pathological tissue due to the pathological tissue being in an acid environment or an alkaline environment.

In the embodiment of the present application, the fixing device further includes a box cover assembly 2 . The box cover assembly 2 is arranged at the upper port position of the box body 1, and can be used to seal the box body 1, so that the box body 1 is in a relatively airtight state during the process of fixing pathological tissues, so as to prevent impurities outside the box body 1 from entering into the Soak the pathological tissue in the fixation solution, and cause pollution to the fixation solution.

Please refer to FIG. 2 , which is a schematic structural diagram of the box body of the fixation device for pathological tissues of the present invention. As shown in the figure, since the box 1 is filled with a fixation solution, the pH of the fixation solution may change during the process of soaking pathological tissues in the fixation solution. Therefore, the box 1 should have certain corrosion resistance. In the embodiment of the present application, the box body 1 can be made of cemented carbide or stainless steel.

In a preferred embodiment, the partition member 11 is connected to the box body 1 in a plug-in type. In this way, the partition member 11 can be reasonably arranged in the box body 1 according to different types and sizes of pathological tissues, so as to divide the box body 1 into chambers of different sizes. In this way, the restriction on the type and size of pathological tissues is avoided, and pathological tissues of different types and sizes can be fixed. In addition, the partition member 11 is connected to the box body 1 in a plug-in type, which makes the installation of the partition member 11 more flexible and facilitates the installation and disassembly of the partition member 11 .

In a preferred embodiment, the partition members 11 are arranged in the box body 1 at equal intervals. In this way, multiple pathological tissues of the same type can be fixed at one time, realizing the purpose of fixing pathological tissues in large quantities. In the embodiment of the present application, a sealing member (not shown in the figure) is provided on the peripheral wall of the partition member 11 . The setting of the sealing member can make the gap between the partition member 11 and the box body 1 be sealed after the partition member 11 is inserted into the box body 1 . In this way, the chambers 12 partitioned by the partition member 11 can be in a relatively airtight state, thereby avoiding the disadvantage of cross-contamination of pathological tissues between two adjacent chambers 12 . In the embodiment of the present application, the sealing member may be a sealing ring or any other component with sealing performance.

In a preferred embodiment, the outer wall of each chamber 12 is provided with a liquid level display part 13 that is visually visible to the operator. The liquid level display part 13 can display the liquid level of the fixed solution in the corresponding chamber 12 at all times, so that it is convenient for the operator to adjust the liquid level of the fixed solution in the chamber 12 in time. Avoiding the disadvantages of overflowing the tank 1 due to too high liquid level in the chamber 12, and avoiding the disadvantages of poor fixation effect on pathological tissues due to too low liquid level.

In a preferred embodiment, an ultraviolet disinfection component 14 is fixedly installed on the inner wall of each chamber 12 . The ultraviolet sterilizing component 14 can emit ultraviolet light, which has strong penetrating power and can irradiate the bacteria or viruses in the fixation solution, so that the bacteria or viruses cannot reproduce due to denaturation, so as to achieve the purpose of sterilizing the pathological tissue. For sterilization and disinfection purposes. In this way, the cleanliness of the pathological tissue is ensured, and the disadvantage of the pathological tissue being rotted due to the propagation of its own virus or bacteria is avoided.

In addition, after the pathological tissue is fixed once, under the action of the ultraviolet sterilizing component 14 , the box body 1 can be sterilized and sterilized. Therefore, the phenomenon of cross-contamination of the pathological tissue to be fixed next time due to the remaining fixation solution used to fix the pathological tissue in the box body 1 last time is prevented.

In the embodiment of the present application, the ultraviolet sterilizing component 14 may be an ultraviolet germicidal lamp. The principle and structure of the ultraviolet germicidal lamp are well known to those skilled in the art, and will not be described in detail here.

In a preferred embodiment, the side wall of each chamber 12 is also provided with a temperature detection component 17 that is visually visible to the operator. The temperature detection component 17 can display the temperature of the fixation solution in the corresponding chamber 12 at all times. Therefore, it is convenient for the operator to control the temperature of the fixation solution in time, avoiding the disadvantages of accelerating the volatilization of the fixation solution due to the high temperature, and avoiding the deterioration of the pathological tissue due to the high temperature of the fixation solution. At the same time, it is avoided that the fixation effect of the pathological tissue is affected due to the temperature of the fixation solution being too low. In the disclosed embodiment, the temperature of the fixation solution in each chamber 12 ranges from 4°C to 20°C. In this way, the volatilization speed of the fixation solution can be slowed down, ensuring the fixation effect of the pathological tissue.

In a preferred embodiment, the side wall of each chamber 12 is also provided with a concentration detection component 18 that is visually visible to the operator. The concentration detecting part 18 can display the concentration of the fixation solution in the corresponding chamber 12 at all times. Therefore, it is convenient for the operator to control the concentration of the fixing solution in time.

In a preferred embodiment, the side wall of each chamber 12 is also provided with a pH detection component 19 that is visually visible to the operator. The pH detection component 19 can display the pH of the fixed solution in the corresponding chamber 12 at all times. Therefore, it is convenient for the operator to control the pH of the fixing solution in time. Once the fixation solution is found to be in an overacid or overbase state, corresponding adjustments can be made in time to ensure that the pH of the fixation solution is neutral. In the embodiment of the present application, since the structures and working principles of the temperature detection component 17 , the concentration detection component 18 and the pH detection component 19 are well known to those skilled in the art, they will not be described in detail here. In the embodiment of the present application, the temperature detection part 17 can be a temperature detector, the concentration detection part 18 can be a concentration detector, and the pH detection part 19 can be a pH detector.

In a preferred embodiment, a drain port 15 is opened on the side wall of each chamber 12 , and a filter screen 151 is fixedly installed on the peripheral wall of the drain port 15 . In this way, after the fixation of the pathological tissue is completed, the fixation solution soaked in the pathological tissue needs to be poured out. Therefore, through the setting of the liquid discharge port 15, the disadvantage of turning over the entire fixation device and pouring out the fixation solution is avoided. The labor intensity of the operator is greatly reduced.

In addition, due to the setting of the filter screen 151, the fixed pathological tissue can be effectively isolated in the corresponding chamber 12, and the discarded fixation solution is poured out, avoiding the waste of the fixation solution and the fixed pathological tissue. The disadvantages of pouring out together.

In a preferred embodiment, the fixing device further includes a sealing cover 16 . The sealing cover 16 cooperates with the liquid discharge port 15, so as to realize the sealing effect on the liquid discharge port 15, and avoid the disadvantage that the fixing solution is discharged from the liquid discharge port 15 during the process of fixing the pathological tissue. More preferably, a connecting pipe 152 protruding from the side wall of the corresponding chamber 12 is provided on the peripheral wall of the liquid discharge port 15 , and the connecting pipe 152 can be threadedly connected with the sealing cover 16 . In this way, the installation and removal of the sealing cover 16 is facilitated, and the labor intensity of the operator is reduced.

Please refer to FIG. 3 , which is a schematic structural diagram of the box cover assembly of the fixation device for pathological tissues of the present invention. As shown in the figure, in a preferred embodiment, an installation opening 21 is opened on the cover assembly 2 corresponding to each chamber 12 (see FIG. 2 ).

In a preferred embodiment, the fixing device further includes a sealing box cover 22 . The sealed box cover 22 is installed in the installation opening 21 and is hinged with the box cover assembly 2 . Specifically, the sealing box cover 22 can be opened and closed relative to one side of the surrounding wall of the installation opening 21 . In this way, it is convenient for the operator to put pathological tissue into or take out pathological tissue into the corresponding chamber 12 . At the same time, in the process of fixing the pathological tissue, the sealed box cover 22 is closed, so that impurities outside the box body 1 (see FIG. 1 ) can be effectively prevented from entering the fixing solution in the corresponding chamber 12, and Affect the fixation effect of pathological tissue. A handle 221 that facilitates the opening and closing of the sealing box cover 22 is also provided on the sealing box cover 22 .

In a preferred embodiment, each sealing box cover 22 is also provided with a liquid injection port 222 .

In the embodiment of the present application, the fixing device further includes a liquid injection funnel 3 that is pluggably matched with the liquid injection port 222 . The setting of the liquid injection funnel 3 can facilitate the operator to inject the fixing solution into the corresponding chamber 12 . Simultaneously, since the fixing solution in the present invention is a neutral buffered formalin solution, the neutral buffered formalin solution has the characteristics of strong volatility. If the injection funnel 3 in the fixture is not used to inject the neutral buffered formalin solution into the chamber 12, but the open mouth is directly injected into the neutral buffered formalin solution in the chamber 12, it will cause volatilization The gas-forming neutral buffered formalin solution is directly sprayed on the operator's face, which will affect the breathing of the operator and affect the health of the operator.

According to a first example of the present invention: according to the type and size of the pathological tissue to be fixed, a partition member 11 is inserted into the box 1 (see FIG. 2 ).

Prepare a fixed solution (neutral buffered formalin solution) with a preset concentration.

After the configuration is completed, the fixing solution is poured into the injection funnel 3 through the cooperation of the injection funnel 3 with the injection port 222 . During the pouring process, the operator needs to observe the liquid level height of the fixation solution displayed by the liquid level display part 13 (see FIG. 2 ), so as to accurately control the liquid level height of the fixation solution required by the pathological tissue to be fixed.

After the injection of the fixative solution is completed, the concentration of the fixative solution is detected by the concentration detection part 18 (see FIG. 2 ), so that the operator can judge whether the concentration of the fixative solution reaches the preset concentration value. Simultaneously, utilize pH detection part 19 (seeing Fig. 2) to detect the pH of fixed solution, to facilitate the operator can judge whether the pH of fixed solution has reached preset pH value in time (the pH of fixed solution should be neutral) . In addition, the temperature of the fixation solution is detected by the temperature detection component 17 (see FIG. 2 ), so that the operator can judge the temperature of the fixation solution in a timely manner.

After the above detection, it is judged that the concentration, temperature and pH of the fixation solution have reached the preset values, and the pathological tissue to be fixed is put into the fixation solution to start fixing the pathological tissue.

Set the preset time required to fix the pathological tissue.

When the preset time is reached, it indicates that the fixation of the pathological tissue has been completed, the discarded fixation solution is poured out, and the fixed pathological tissue is taken out.

In summary, the fixation device has the advantages of avoiding cross-contamination between pathological tissues, having a good fixation effect on pathological tissues, and making the same batch of pathological tissues have the same fixation effect, and also has the advantages of being able to detect in real time Advantages of solution concentration, temperature and pH.

Please refer to FIG. 4 , which is a schematic flowchart of a method for fixing pathological tissues by the fixing device of the present invention. As shown in the figure, the method includes the following steps:

In step S410, a fixed solution with a preset concentration is prepared, and the preset concentration ranges from 9.5% to 10%.

Step S411, after finishing the configuration of the fixation solution, inject the fixation solution into the corresponding chamber of the box, and adjust the size of the chamber in the box appropriately according to the type and size of the pathological tissue to be fixed.

Step S412, using the concentration detection component to detect whether the configured fixation solution reaches a preset concentration value.

Step S413, if the preset concentration value is not reached, the concentration of the fixative solution is adjusted, that is, if the concentration value of the fixative solution at this time is lower than the preset concentration value, a certain proportion of the fixative solution can be injected into the fixative solution , to reach the preset density value. If the concentration of the fixative solution at this time is higher than the preset concentration value, a solvent (such as water) can be injected into the fixative solution to achieve the purpose of diluting the fixative solution, so that the concentration of the fixative solution reaches the preset concentration value .

The step S413 further includes, using the temperature detection component to detect the temperature of the fixation solution to determine whether the fixation solution has reached a preset temperature value, and the preset temperature value ranges from 4°C to 20°C. At the same time, the pH detection part is used to complete the detection of the pH of the fixation solution, so as to judge whether the fixation solution reaches a neutral environment.

Step S414, after the above detection, it can be judged that the temperature, concentration and pH of the fixation solution have reached the preset values, then put the pathological tissue to be fixed into the chamber filled with the fixation solution.

In step S415, according to different types of pathological tissues, the preset time required for fixing the pathological tissues is set, and the pathological tissues are fixed.

Step S416, when the preset time is reached, it indicates that the fixation of the pathological tissue is completed, and the waste fixation solution is discharged from the drain opening on the side wall of the box, and the pathological tissue is taken out. At the same time, the box is sterilized and sterilized by using ultraviolet disinfection components to avoid the disadvantage of cross-contamination to the pathological tissue that needs to be fixed next time. In the embodiment of the present application, the settings of the preset time, the preset concentration value and the preset temperature value are well known to those skilled in the art and will not be described in detail here.

While the invention has been described with reference to a preferred embodiment, various modifications may be made and equivalents may be substituted for parts thereof without departing from the scope of the invention. In particular, as long as there is no structural conflict, the technical features mentioned in the various embodiments can be combined in any manner. The present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims (12)

1. A fixation device for pathological tissue, comprising: A box for containing fixative solution and pathological tissue, and a partition part is arranged at intervals in the box to divide the box into multiple chambers; A box cover assembly is arranged at the upper port of the box body, and the box cover assembly is used to seal the box body. 2 . The fixation device for pathological tissue according to claim 1 , wherein the partition member is connected to the box in a plug-in type. 3 . 3. The fixing device for pathological tissue according to claim 2, characterized in that, the partition members are arranged in the box at equal intervals. 4 . The fixation device for pathological tissue according to claim 3 , wherein a liquid level display member capable of displaying the level of the fixation solution is provided on the outer wall of each chamber. 5 . 5. The fixing device for pathological tissue according to claim 3, characterized in that, an ultraviolet sterilizing component for sterilizing the pathological tissue in the chamber is fixedly installed on the inner side wall of each chamber. 6. The fixing device for pathological tissue according to claim 4 or 5, characterized in that, a drain port is provided on the side wall of each chamber, and the peripheral wall of the drain port is fixed and installed usefully. A filter for filtering the pathological tissue. 7. The fixation device for pathological tissue according to claim 6, characterized in that, the fixation device further comprises a sealing cover that cooperates with the liquid discharge port and is used to seal the liquid discharge port. 8. The fixation device for pathological tissue according to claim 3, characterized in that, the side walls of each chamber are also provided with: a temperature detecting part for detecting the temperature of the fixing solution; a concentration detecting part for detecting the concentration of the fixed solution; A pH detection part for detecting the pH of the fixative solution. 9 . The fixation device for pathological tissue according to claim 1 , wherein an installation opening is opened on the cover assembly corresponding to each of the chambers. 10 . 10. The fixing device for pathological tissue according to claim 9, characterized in that, the fixing device further comprises a sealing box cover installed in the installation opening, and the sealing box cover is connected with the box cover assembly hinged. 11. The fixation device for pathological tissue according to claim 10, wherein a liquid injection port is opened on each of the sealed box covers. 12. A method for fixing a pathological tissue with a fixation device, the fixation device comprising a casing, the method comprising: Prepare a fixed solution with a preset concentration; After the fixative solution is prepared, it is injected into the corresponding chamber of the box; Detect whether the configured fixative solution reaches the preset concentration value; If the preset concentration value is not reached, the concentration of the fixed solution is adjusted to reach the preset concentration value; If the preset concentration value has been reached, put the pathological tissue to be fixed into the chamber filled with the fixation solution; Set the preset time required for fixing the pathological tissue, and start fixing the pathological tissue; When the preset time is reached, it indicates that the pathological tissue fixation is completed.