CN104498348A - Filter used for separating specific cells - Google Patents
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- CN104498348A CN104498348A CN201410801841.2A CN201410801841A CN104498348A CN 104498348 A CN104498348 A CN 104498348A CN 201410801841 A CN201410801841 A CN 201410801841A CN 104498348 A CN104498348 A CN 104498348A
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- 238000001514 detection method Methods 0.000 claims abstract description 83
- 238000001914 filtration Methods 0.000 claims abstract description 55
- 239000007788 liquid Substances 0.000 claims abstract description 42
- 239000012528 membrane Substances 0.000 claims description 60
- 210000004027 cell Anatomy 0.000 claims description 32
- 239000012530 fluid Substances 0.000 claims description 22
- 238000009825 accumulation Methods 0.000 claims description 5
- 210000002421 cell wall Anatomy 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 7
- 238000012544 monitoring process Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000002360 explosive Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003679 cervix uteri Anatomy 0.000 description 1
- 210000005266 circulating tumour cell Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011900 installation process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 210000004910 pleural fluid Anatomy 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
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- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses a filter used for separating specific cells. The filter sequentially comprises a volume tube, a liquid level detection tube and a filtering device according to a sample flowing direction, wherein the volume tube is a hollow cylinder and an inlet is formed in the first end of the volume tube and is used for inputting and storing a sample; the liquid level detection tube is a transparent tube; the first end of the liquid level detection tube is connected with the second end of the volume tube and the liquid level detection tube is used for inputting and storing the sample input by the volume tube, and detecting and the height of a liquid level; the first end of the filtering device is connected with the second end of the liquid level detection tube; and an outlet is formed in the second end of the filtering device and is used for filtering the specific cells in the sample and outputting the filtered sample. According to the filter disclosed by the invention, after the liquid level of liquid in the liquid level detection tube reaches a photoelectric monitoring point, light is deviated by the refraction of the light and is used for detecting the height of the liquid level, so that the filtering device keeps in the liquid all the time, and the filtered cells keep the activity.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of filter for separating of specific cell.
Background technology
Part specific cell quantity in peripheral blood is few, as circulating tumor cell (CTCs), usually needs to find only several tumour cell in about 100,000,000 white corpuscles or 50,000,000,000 red corpuscle.At ascites pleural fluid, urine, sputum, uterine cervix is scrubbed in the body fluid such as liquid and bronchial perfusate also there is the specific cells such as tumour, the common feature of specific cell is that relative body fluid cell volume is bigger than normal, quantity is few, therefore when using particular device to detect, in order to ensure validity and the accuracy of equipment use, must filter cell.
Filtration method is the separation method based on cell size, is mainly separated specific cell (as tumour cell) according to cell with Normocellular size.This cellular form by physical method for separation is preserved complete, and the various antigen on surface or molecule marker are all without destruction, and do not affect the characteristic of cell, for subsequent detection provides good condition, and the method is less demanding to equipment and technology, and process is also easy to grasp.
Use filter to be filter membrane and corresponding volume tube or syringe are simply linked together mostly in current filtration method, there is following defect: operation steps is various, be not easy to control; The easy fold breakage of filter membrane can not ensure filter effect; Filter membrane easily exposes in atmosphere, can not ensure the original characteristic of specific cell.
Summary of the invention
For this reason, the present invention proposes a kind of filter for separating of specific cell that is that can solve the problem or that at least can partly solve the problem.
According to an aspect of the present invention, provide a kind of filter for separating of specific cell, this filter flows to per sample and comprises successively:
Volume tube, described volume tube is hollow circuit cylinder, its first end inlet porting, for input and accumulation sample;
Level Detection pipe, described Level Detection pipe is clear tubular, and its first end is connected with described volume tube second end, for inputting, accumulating the sample of described volume tube input, and for detecting the height of liquid level;
Filtration unit, described filtration unit first end is connected with described Level Detection pipe second end, and described filtration unit second end arranges outlet, for the specific cell in filtered sample and by the sample output after filtration.
Alternatively, according to filter of the present invention, wherein, described volume tube second end and described Level Detection pipe first end junction are provided with the first seal washer.
Alternatively, according to filter of the present invention, wherein said volume tube second end is connected by buckle structure and guide frame with described Level Detection pipe first end.
Alternatively, according to filter of the present invention, wherein said Level Detection pipe first end is sleeved in described volume tube second end, wherein,
Described Level Detection pipe first end outer wall arranges depression or the buckle structure of projection and the guide frame of depression or projection;
Described volume tube second end inwall is provided with the buckle structure and guide frame that match with described Level Detection pipe first end outer wall.
Alternatively, according to filter of the present invention, wherein said filtration unit comprises:
Inner pallet, described inner pallet first end is provided with circular groove, and the second end is provided with outlet, and described inner pallet is sleeved in described Level Detection pipe second end by the cell wall of described circular groove; And,
Be arranged on the fluid control disk in described circular groove, supporting network, filter membrane and the second seal washer successively.
Alternatively, according to filter of the present invention, wherein said filtration unit comprises check nut further, and described check nut to be sleeved on described liquid level detector second end outer wall and to be locked by screw thread.
Alternatively, according to filter of the present invention, wherein said inner pallet is connected by guide frame with described Level Detection pipe second end, wherein,
The outer wall of described circular groove is provided with raised or sunken guide frame, described Level Detection pipe second end inwall is provided with the guide frame of depression or the projection coordinated with described guide frame.
Alternatively, according to filter of the present invention, the aperture of wherein said filter membrane is 5-50um, thickness is 3-100um, area is 5mm
2-500mm
2, and there are 10-30 ten thousand filter openings.
Alternatively, according to filter of the present invention, wherein said Level Detection bore is 6-20um, length is 20-60um.
Alternatively, according to filter of the present invention, wherein said fluid control disk is provided with the filter opening that aperture is 0.2-10mm.
According to filter of the present invention, filter membrane is fixed in Level Detection pipe, testing sample enters in volume tube and Level Detection pipe, flow out from the outlet of inner pallet after membrane filtration, specific cell is trapped within filter membrane, after filtration completes, the filter operation new from wherein taking out and installing new filter membrane by filter membrane.According to filter of the present invention, Level Detection pipe is clear tubular, it is connected with test set, according to the liquid level of internal liquid through photoelectric monitor, by refraction of light, light is offset and carry out detection liquid level, can control liquid level according to detected result, thus filter membrane is kept in the sample to which always, the specific cell that filtration is obtained can keep its original characteristic.According to filter of the present invention, being provided with fluid control disk in its filtration unit is arranged on inner pallet, can the flow velocity of Quality control and flow, in addition supporting network is set in filter membrane bottom, and filter membrane top arranges the breakage that impact that seal washer all can prevent filter membrane to be subject to sample causes; Connected by buckle between the outer wall and Level Detection pipe second end inwall of inner pallet circular groove, avoid the revolving force be threaded, prevent filter membrane fold and breakage, ensure that completing smoothly of filter operation.
Accompanying drawing explanation
By reading hereafter detailed description of the preferred embodiment, other advantages various and benefit will become cheer and bright for those of ordinary skill in the art.Accompanying drawing only for illustrating the object of preferred implementation, and does not think limitation of the present invention.In the accompanying drawings:
Fig. 1 shows the filter according to one embodiment of the present invention;
Fig. 2 shows the filter explosive view according to one embodiment of the present invention;
Fig. 3 shows the volume tube stereographic map according to one embodiment of the present invention;
Fig. 4 shows the liquid level detector stereographic map according to one embodiment of the present invention;
Fig. 5 shows the inner pallet front view according to one embodiment of the present invention;
Fig. 6 shows the vertical view of the check nut according to one embodiment of the present invention;
Fig. 7 shows the vertical view of the fluid control disk according to one embodiment of the present invention;
Wherein, Reference numeral is:
Filter 1000, volume tube 1100, Level Detection pipe 1200, filtration unit 1300, inner pallet 1310, circular groove 1311, outlet 1312, fluid control disk 1320, supporting network 1330, filter membrane 1340, second seal washer 1350, check nut 1360 and the first seal washer 1400.
Embodiment
The invention provides many applicable creative concepts, this creative concept can be reflected in a large number of in concrete context.The specific embodiment described in following embodiments of the present invention only as the exemplary illustration of specific implementation of the present invention, and does not form limitation of the scope of the invention.
According to the filter 1000 for separating of specific cell of the present invention, this filter 1000 flows to per sample and comprises successively: volume tube 1100, and volume tube 1100 is hollow circuit cylinder, its first end inlet porting, for input and accumulation sample; Level Detection pipe 1200, Level Detection pipe 1200 is clear tubular, and its first end is connected with volume tube 1,100 second end, for inputting, accumulating the sample that volume tube 1100 inputs, and for detecting the height of liquid level; Filtration unit 1300, filtration unit 1300 first end is connected with Level Detection pipe 1,200 second end, and filtration unit 1,300 second end arranges outlet, for the specific cell in filtered sample and by the sample output after filtration.
According to filter of the present invention, testing sample enters in volume tube and Level Detection pipe, and device flows out from the outlet of inner pallet after filtering after filtration, and specific cell is trapped within filter membrane, after filtration completes, the filter operation new from wherein taking out and installing new filter membrane by filter membrane; According to filter of the present invention, Level Detection pipe is clear tubular, it is connected with test set, according to the liquid level of internal liquid through photoelectric monitor, by refraction of light, light is offset to carry out detection liquid level, can liquid level be controlled according to detected result, thus filter membrane is kept in the sample to which always, isolated air, the specific cell that filtration is obtained can keep its original characteristic.
According to filter 1000 of the present invention, wherein, volume tube 1,100 second end and Level Detection pipe 1200 first end junction are provided with the first seal washer 1400.
According to filter of the present invention, wherein volume tube can make after the first seal washer is set with the junction of described Level Detection pipe both be connected tightr.
According to filter 1000 of the present invention, wherein volume tube 1,100 second end is connected by buckle structure and guide frame with Level Detection pipe 1200 first end.
According to filter 1000 of the present invention, wherein Level Detection pipe 1200 first end is sleeved in volume tube 1,100 second end, wherein,
Level Detection pipe 1200 first end outer wall arranges depression or the buckle structure of projection and the guide frame of depression or projection;
Volume tube 1,100 second end inwall is provided with the buckle structure and/or guide frame that match with Level Detection pipe 1200 first end outer wall.
According to filter of the present invention, the mode of connection wherein between Level Detection pipe and volume tube adopts buckle structure to be connected with guide frame.Wherein, both only can use buckle structure, as arranged protruding buckle structure at Level Detection pipe first end, then volume tube second end inwall depression is set or the buckle structure of Openworks shape, the structure of described projection and the structure of depression are fastened togather, otherwise, Level Detection pipe first end is set to the buckle structure caved in, then volume tube second end arranges protruding buckle structure, if only arrange buckle structure, then at least arranges 3.Preferably, buckle structure is set simultaneously and is connected with guide frame, when to arrange buckle structure and guide frame simultaneously, connect more fastening, connect better effects if, and avoid the revolving force produced when adopting screw threads for fastening.
According to filter 1000 of the present invention, wherein filtration unit 1300 comprises:
Inner pallet 1310, inner pallet 1310 first end is provided with circular groove 1311, and the second end is provided with outlet 1312, and inner pallet 1310 is sleeved in Level Detection pipe 1,200 second end by the cell wall of circular groove 1311; And,
Be arranged on the fluid control disk 1320 in circular groove 1311, supporting network 1330, filter membrane 1340 and the second seal washer 1350 successively.
According to filter of the present invention, on the circular groove inner fluid controlling board of described inner pallet and between filter membrane, supporting network is set, filter membrane is laid on supporting network, filter membrane is provided support, even if when sample to be separated forms impact to filter membrane, also can ensure that filter membrane there will not be breakage, thus ensure carrying out smoothly of filter operation.The edge of the second seal washer is pressed on filter membrane, when filtered sample drops on filter membrane, sample can be made to fall on filter membrane filter membrane can not be made to produce fold thus affect filter effect.Wherein in filtration unit, be provided with fluid control disk, can by the aperture size of fluid control disk and the flow velocity of filter opening distributed controll fluid and flow.
According to filter 1000 of the present invention, wherein filtration unit 1300 comprises check nut 1360 further, and check nut 1360 to be sleeved on liquid level detector 1,200 second end outer wall and to be locked by screw thread.
Inwall according to check nut of the present invention is provided with screw thread, the outer wall of liquid level detector second end is provided with the screw thread coordinated with check nut lock to facilitate, lock at its outer wall, thus the power that in guarantee inner pallet, filter membrane is only upwards pushed away, thus fold can not be produced ensure that filter effect.
According to filter 1000 of the present invention, wherein inner pallet 1310 is connected by guide frame with Level Detection pipe 1,200 second end, wherein,
The outer wall of circular groove 1311 is provided with raised or sunken guide frame, Level Detection pipe 1,200 second end inwall is provided with the guide frame of depression or the projection coordinated with guide frame.
According to filter of the present invention, wherein, the guide frame circular groove of inner pallet arranging raised or sunken guide frame and arrange the depression corresponding with it or projection on the inwall of Level Detection pipe second end carries out connection to both and seals, filter membrane is made only to bear the pressure of seal washer, and the revolving force etc. that when preventing spiral to tighten, screw thread produces, thus ensure that filter membrane can not produce fold in the process of installing, and ensure that the effect of filtration.
According to filter 1000 of the present invention, the aperture of wherein said filter membrane 1340 is 5-50um, thickness is 3-100um, area is 5mm
2-500mm
2, and there are 10-30 ten thousand filter openings.
According to filter of the present invention, utilize filter membrane area for 5-500mm
2filter membrane, during 10-30 ten thousand filter opening, detect 1-10ml blood, recall rate can reach 80%; And usable floor area is 0.5-5mm
2, 1000-10 ten thousand filter opening the blood of filter membrane to 0.1-2.5ml detect, recall rate is only 20-50%.
According to filter 1000 of the present invention, wherein Level Detection pipe 1200 internal diameter is 6-20um, length is 20-60um.
Wherein according to filter of the present invention, wherein the internal diameter of Level Detection pipe is less than the internal diameter of volume tube.
According to filter 1000 of the present invention, wherein fluid control disk 1320 is provided with aperture is 0.2-10mm filter opening.
According to filter of the present invention, wherein fluid control disk arranges filter opening, and the size in filter opening aperture arranges different specifications according to the control of flow velocity and flow.
Filter optional element according to the present invention is more, unitized design can go out different embodiments according to claim of the present invention, but different embodiments only the present invention will be described, be not to limit the invention.Below in conjunction with drawings and Examples, the present invention is described further.
Embodiment 1
The filter 1000 for separating of specific cell according to Fig. 1, this filter 1000 flows to per sample and comprises successively: volume tube 1100, and volume tube 1100 is hollow circuit cylinder, its first end inlet porting, for input and accumulation sample; Level Detection pipe 1200, Level Detection pipe 1200 is clear tubular, and its first end is connected with described volume tube 1,100 second end, for inputting, accumulating the sample that volume tube 1100 inputs, and for detecting the height of liquid level; Filtration unit 1300, filtration unit 1300 first end is connected with Level Detection pipe 1,200 second end, and filtration unit 1,300 second end arranges outlet, for the specific cell in filtered sample and by the sample output after filtration.
According to the filter of the present embodiment, testing sample enters in volume tube and Level Detection pipe, and device flows out from the outlet of inner pallet after filtering after filtration, and specific cell is trapped within filter membrane, after filtration completes, the filter operation new from wherein taking out and installing new filter membrane by filter membrane; According to the filter of the present embodiment, Level Detection pipe is clear tubular, it is connected with test set, according to the liquid level of internal liquid through photoelectric monitor, by refraction of light, light is offset to carry out detection liquid level, can liquid level be controlled according to detected result, thus filter membrane is kept in a liquid always, isolated air, the specific cell that filtration is obtained can keep its original characteristic.
Embodiment 2
The filter 1000 for separating of specific cell according to Fig. 1, this filter 1000 flows to per sample and comprises successively: volume tube 1100, and volume tube 1100 is hollow circuit cylinder, its first end inlet porting, for input and accumulation sample; Level Detection pipe 1200, Level Detection pipe 1200 is clear tubular, and its first end is connected with described volume tube 1,100 second end, for inputting, accumulating the sample that volume tube 1100 inputs, and for detecting the height of liquid level; Filtration unit 1300, filtration unit 1300 first end is connected with Level Detection pipe 1,200 second end, and filtration unit 1,300 second end arranges outlet, for the specific cell in filtered sample and by the sample output after filtration.
Can find out according to the explosive view of filter 1000 of the present invention according to Fig. 2, wherein, volume tube 1,100 second end and Level Detection pipe 1200 first end junction are provided with the first seal washer 1400, and wherein volume tube 1,100 second end is connected by buckle structure and guide frame with Level Detection pipe 1200 first end.Level Detection pipe 1200 first end is sleeved in volume tube 1,100 second end, and wherein, Level Detection pipe 1200 first end outer wall arranges depression or the buckle structure of projection and the guide frame of depression or projection; Described volume tube 1,100 second end inwall is provided with the buckle structure and guide frame that match with Level Detection pipe 1200 first end outer wall.Filtration unit 1300 comprises: inner pallet 1310, and inner pallet 1310 first end is provided with circular groove 1311, and the second end is provided with outlet 1312, and inner pallet 1310 is sleeved in Level Detection pipe 1,200 second end by the cell wall of circular groove 1311; And, be arranged on the fluid control disk 1320 in circular groove 1311, supporting network 1330 and filter membrane 1340 successively; And the second seal washer 1350, second seal washer 1350 is arranged on Level Detection pipe 1,200 second end and inner pallet 1310 junction.Inner pallet 1310 is connected by guide frame with Level Detection pipe 1,200 second end, wherein, the outer wall of circular groove 1311 is provided with raised or sunken guide frame, Level Detection pipe 1,200 second end inwall is provided with the guide frame of depression or the projection coordinated with guide frame.Filtration unit 1300 comprises check nut 1360 further, and check nut 1360 to be sleeved on liquid level detector 1,200 second end outer wall and to be locked by screw thread.The aperture of filter membrane 1340 is 5-50um, thickness is 3-100um, area is 5mm
2-500mm
2, and there are 10-30 ten thousand filter openings.Level Detection pipe 1200 internal diameter is 6-20um, length is 20-60um; It is 0.2-10um filter opening that fluid control disk 1320 is provided with aperture.
The three-dimensional arrangement of volume tube and Level Detection pipe wherein can be found out according to Fig. 3 and Fig. 4, and buckle structure on volume tube and Level Detection pipe and guide frame.As can be seen from Fig. 3 and 4, buckle structure and guide frame are set simultaneously in the present embodiment, wherein, Level Detection pipe first end arranges protruding buckle structure, the buckle structure of Openworks shape is set at volume tube second end inwall, the structure of protruding structure and depression is fastened togather, and the first end of Level Detection pipe is provided with protruding guide frame, and the guide frame that the second end inwall of volume tube arranges depression matches with it.
Can find out that inner pallet comprises circular groove 1311 and outlet 1312 according to Fig. 5; Can find out that check nut inside is provided with screw thread according to Fig. 6, outer setting bulge-structure assists check nut rotate and be tightened on described liquid level detector second end outer wall; As can be seen from Figure 7 fluid control disk is provided with filter opening, also can selects the fluid control disk with different size filter opening as required.
According to the filter of the present embodiment, testing sample enters in volume tube and Level Detection pipe, and device flows out from the outlet of inner pallet after filtering after filtration, and specific cell is trapped within filter membrane, after filtration completes, by filter membrane new operation from wherein taking out and installing new filter membrane.According to the filter of the present embodiment, Level Detection pipe is clear tubular, it is connected with test set, according to the liquid level of internal liquid through photoelectric monitor, by refraction of light, light is offset to detect liquid level, control liquid level according to detected result, thus filter membrane is kept in the sample to which always, isolated air, the specific cell that filtration is obtained can keep its primary characteristic.On the circular groove inner fluid controlling board of inner pallet and between filter membrane, supporting network is set, filter membrane is laid on supporting network, filter membrane is provided support, even if when sample to be separated forms impact to filter membrane, also can ensure that filter membrane there will not be breakage, thus ensure carrying out smoothly of filter operation.Second seal washer edge is pressed on filter membrane, when filtered sample drops on filter membrane, sample can be made to fall on filter membrane filter membrane can not be made to produce fold.Fluid control disk is provided with in filtration unit, can by the aperture size of fluid control disk and the flow velocity of filter opening distributed controll fluid and flow.The circular groove of inner pallet arranges raised or sunken guide frame, make filter membrane in installation process, only bear the pressure of seal washer, and prevent the revolving force etc. as screw thread when spiral is tightened produces, thus ensure that filter membrane can not produce fold in the process of installing, and ensure that the effect of filtration.The outer wall of liquid level detector second end is provided with the screw thread coordinated with check nut lock to facilitate, makes the power that filter membrane in pallet is only upwards pushed away, can not fold be produced.Filter according to the present embodiment can reach more than 80% to the recall rate of specific cell.
It should be noted, the present invention will be described instead of limit the invention for above-described embodiment, and those skilled in the art can design alternative embodiment when not departing from claims scope.In the claims, any reference symbol between bracket should be configured to limitations on claims.Word " comprises " not to be got rid of existence and does not arrange element in the claims or step.
Claims (10)
1. the filter for separating of specific cell (1000), this filter (1000) flows to per sample and comprises successively:
Volume tube (1100), described volume tube (1100) is hollow circuit cylinder, its first end inlet porting, for input and accumulation sample;
Level Detection pipe (1200), described Level Detection pipe (1200) is clear tubular, its first end is connected with described volume tube (1100) second end, for inputting, accumulating the sample that described volume tube (1100) inputs, and for detecting the height of liquid level;
Filtration unit (1300), described filtration unit (1300) first end is connected with described Level Detection pipe (1200) second end, described filtration unit (1300) second end arranges outlet, for the specific cell in filtered sample and by the sample output after filtration.
2. filter according to claim 1 (1000), wherein, described volume tube (1100) second end and described Level Detection pipe (1200) first end junction are provided with the first seal washer (1400).
3. filter according to claim 1 (1000), wherein said volume tube (1100) second end is connected by buckle structure and guide frame with described Level Detection pipe (1200) first end.
4. filter according to claim 3 (1000), wherein said Level Detection pipe (1200) first end is sleeved in described volume tube (1100) second end, wherein,
Described Level Detection pipe (1200) first end outer wall arranges depression or the buckle structure of projection and the guide frame of depression or projection;
Described volume tube (1100) second end inwall is provided with the buckle structure and guide frame that match with described Level Detection pipe (1200) first end outer wall.
5. filter according to claim 1 (1000), wherein said filtration unit (1300) comprising:
Inner pallet (1310), described inner pallet (1310) first end is provided with circular groove (1311), second end is provided with outlet (1312), and described inner pallet (1310) is sleeved in described Level Detection pipe (1200) second end by the cell wall of described circular groove (1311); And,
Be arranged on the fluid control disk (1320) in described circular groove (1311), supporting network (1330), filter membrane (1340) and the second seal washer (1350) successively.
6. filter according to claim 5 (1000), wherein said filtration unit (1300) comprises check nut (1360) further, and described check nut (1360) to be sleeved on described liquid level detector (1200) second end outer wall and to be locked by screw thread.
7. filter according to claim 6 (1000), wherein said inner pallet (1310) is connected by guide frame with described Level Detection pipe (1200) second end, wherein,
The outer wall of described circular groove (1311) is provided with raised or sunken guide frame, described Level Detection pipe (1200) second end inwall is provided with the guide frame of depression or the projection coordinated with described guide frame.
8. filter according to claim 5 (1000), the aperture of wherein said filter membrane (1340) is 5-50um, thickness is 3-100um, area is 5mm
2-500mm
2, and there are 10-30 ten thousand filter openings.
9. filter according to claim 1 (1000), wherein said Level Detection pipe (1200) internal diameter is 6-20um, length is 20-60um.
10. filter according to claim 6 (1000), wherein said fluid control disk (1320) is provided with the filter opening that aperture is 0.2-10mm.
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| CN201410801841.2A CN104498348A (en) | 2014-12-19 | 2014-12-19 | Filter used for separating specific cells |
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|---|---|---|---|
| CN201410801841.2A CN104498348A (en) | 2014-12-19 | 2014-12-19 | Filter used for separating specific cells |
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| CN104498348A true CN104498348A (en) | 2015-04-08 |
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| CN201410801841.2A Pending CN104498348A (en) | 2014-12-19 | 2014-12-19 | Filter used for separating specific cells |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105647784A (en) * | 2016-03-08 | 2016-06-08 | 闫维新 | Negative pressure based specific cell treatment device |
| CN107772751A (en) * | 2017-10-17 | 2018-03-09 | 深圳市镭起科技有限公司 | Essential oil capsules and its method being used cooperatively with steam hair-care comb |
| CN108325388A (en) * | 2018-04-18 | 2018-07-27 | 武汉芯生生物科技有限公司 | A kind of filter for detaching circulating tumor cell |
| CN109266529A (en) * | 2018-10-11 | 2019-01-25 | 武汉友芝友医疗科技股份有限公司 | A kind of cell filter structure |
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| CN105647784A (en) * | 2016-03-08 | 2016-06-08 | 闫维新 | Negative pressure based specific cell treatment device |
| CN107772751A (en) * | 2017-10-17 | 2018-03-09 | 深圳市镭起科技有限公司 | Essential oil capsules and its method being used cooperatively with steam hair-care comb |
| CN108325388A (en) * | 2018-04-18 | 2018-07-27 | 武汉芯生生物科技有限公司 | A kind of filter for detaching circulating tumor cell |
| CN109266529A (en) * | 2018-10-11 | 2019-01-25 | 武汉友芝友医疗科技股份有限公司 | A kind of cell filter structure |
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Application publication date: 20150408 |