CN104490987A - Method for extracting general flavone from soybean leaves - Google Patents
Method for extracting general flavone from soybean leaves Download PDFInfo
- Publication number
- CN104490987A CN104490987A CN201410710444.4A CN201410710444A CN104490987A CN 104490987 A CN104490987 A CN 104490987A CN 201410710444 A CN201410710444 A CN 201410710444A CN 104490987 A CN104490987 A CN 104490987A
- Authority
- CN
- China
- Prior art keywords
- soybean leaves
- eluent
- alcoholic solution
- extracting
- macroporous resin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 34
- 244000068988 Glycine max Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 20
- 229930003944 flavone Natural products 0.000 title claims abstract description 18
- 235000011949 flavones Nutrition 0.000 title claims abstract description 18
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title abstract description 7
- 150000002212 flavone derivatives Chemical class 0.000 title abstract description 7
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title abstract description 7
- 239000000284 extract Substances 0.000 claims abstract description 23
- 239000011347 resin Substances 0.000 claims abstract description 19
- 229920005989 resin Polymers 0.000 claims abstract description 19
- 239000003480 eluent Substances 0.000 claims abstract description 17
- 238000001035 drying Methods 0.000 claims abstract description 7
- 230000001476 alcoholic effect Effects 0.000 claims description 28
- 150000002213 flavones Chemical class 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 10
- 238000010828 elution Methods 0.000 claims description 7
- 238000001179 sorption measurement Methods 0.000 claims description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 4
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000003808 methanol extraction Methods 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting general flavone from soybean leaves. The method comprises the following steps: extracting the soybean leaves by virtue of an ethanol solution to obtain an extracting solution, putting the extracting solution on a column by virtue of macroporous resin absorption, eluting to obtain an eluent, collecting the eluent, concentrating the eluent, and drying, thereby obtaining the product. The method disclosed by the invention has the beneficial effects that 1, the ethanol solution serves as the extracting solution and the eluent and is free of pollution to the environment and products, and green, and the product quality is high; 2, the steps are simple, the operating time is short, the investment is low, and the cost is low; and 3, the content of the general flavone in the obtained extract is high, the yield is high, and the economic benefits are good.
Description
Technical field
The present invention relates to the preparation method of a Plant Extracts, be specifically related to a kind of method extracting total flavones from Soybean Leaves.
Background technology
Total flavones is a kind of important active ingredient contained in Soybean Leaves, has protection cardiovascular system, blood pressure lowering and antiinflammatory, antioxidation, can be used for disease such as treatment hypertension, diabetes etc.In the past is mostly that Bian methanol extraction or water extraction obtain extractive of general flavone from Soybean Leaves, on this basis can also in conjunction with additional physical field strengthening effects such as ultrasonic, microwaves, the shortcomings such as but these extracting method often exist, and yield is low, energy consumption is high, operation is many, toxicity greatly, is easily gone mouldy, mostly containing plurality of impurities in the total flavones crude extract obtained, cause its quality to decline, reduce its medical value.Therefore adopting effective extracting method, extract highly purified extractive of general flavone from Soybean Leaves, is the main direction of studying of this area.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method extracting total flavones from Soybean Leaves, has the advantage of science environmental protection, extract quality better.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions:
From Soybean Leaves, extract a method for total flavones, be by Soybean Leaves after alcoholic solution extracts, with macroporous resin adsorption by extracting solution through upper prop, eluting, collection eluent, eluent is concentrated, obtain product after drying.
In technique scheme:
Described Soybean Leaves volumetric concentration is the alcoholic solution extraction of 30% ~ 80%, and altogether extract three times, the mass ratio of each Soybean Leaves and alcoholic solution is 1:3 ~ 10, extracts 0.1 ~ 1 hour at every turn; Further, the concentration of alcoholic solution is preferably 55%, and the mass ratio of Soybean Leaves and alcoholic solution is preferably 1:5, and each time of extracting is preferably 0.5 hour.
Described macroporous resin is any one in AB-8, HZ818, HPD-100 and D101;
The flow velocity that described extracting solution flows through macroporous resin is 1 ~ 3mL/min, is preferably 2mL/min;
During described eluting, selective body volume concentrations is the alcoholic solution of 50% ~ 95% is eluant, and elution flow rate is 1 ~ 4mL/min; Further, the concentration of alcoholic solution is preferably 75%, and elution flow rate is preferably 2.5mL/min.
The invention has the beneficial effects as follows:
1, the present invention take alcoholic solution as extracting solution and eluent, to environment and product pollution-free, environmental protection, product quality is high;
2, step of the present invention is simple, and the operating time is short, small investment, and cost is low;
3, the extractive of general flavone of the present invention's acquisition, general flavone content can reach more than 40%, and yield is high, good in economic efficiency.
Detailed description of the invention
Be described further with embodiment below, but the present invention is not limited to these embodiments.
Embodiment 1
From Soybean Leaves, extract a method for total flavones, be by Soybean Leaves after alcoholic solution extracts, with macroporous resin adsorption by extracting solution through upper prop, eluting, collection eluent, eluent is concentrated, obtain product after drying.
Wherein, Soybean Leaves volumetric concentration is the alcoholic solution extraction of 60%, and altogether extract three times, the mass ratio of each Soybean Leaves and alcoholic solution is 1:8, each extraction 0.5 hour; The model of macroporous resin is AB-8; The flow velocity that extracting solution flows through macroporous resin is 2mL/min; During eluting, selective body volume concentrations is the alcoholic solution of 75% is eluant, and elution flow rate is 2.5mL/min.
Embodiment 2
From Soybean Leaves, extract a method for total flavones, be by Soybean Leaves after alcoholic solution extracts, with macroporous resin adsorption by extracting solution through upper prop, eluting, collection eluent, eluent is concentrated, obtain product after drying.
Wherein, Soybean Leaves volumetric concentration is the alcoholic solution extraction of 30%, and altogether extract three times, the mass ratio of each Soybean Leaves and alcoholic solution is 1:7, each extraction 1 hour; The model of macroporous resin is HZ818; The flow velocity that extracting solution flows through macroporous resin is 1mL/min; During eluting, selective body volume concentrations is the alcoholic solution of 70% is eluant, and elution flow rate is 4mL/min.
Embodiment 3
From Soybean Leaves, extract a method for total flavones, be by Soybean Leaves after alcoholic solution extracts, with macroporous resin adsorption by extracting solution through upper prop, eluting, collection eluent, eluent is concentrated, obtain product after drying.
Wherein, Soybean Leaves volumetric concentration is the alcoholic solution extraction of 75%, and altogether extract three times, the mass ratio of each Soybean Leaves and alcoholic solution is 1:10, each extraction 0.1 hour; The model of macroporous resin is HPD-100; The flow velocity that extracting solution flows through macroporous resin is 2mL/min; During eluting, selective body volume concentrations is the alcoholic solution of 95% is eluant, and elution flow rate is 1mL/min.
Embodiment 4
From Soybean Leaves, extract a method for total flavones, be by Soybean Leaves after alcoholic solution extracts, with macroporous resin adsorption by extracting solution through upper prop, eluting, collection eluent, eluent is concentrated, obtain product after drying.
Wherein, Soybean Leaves volumetric concentration is the alcoholic solution extraction of 80%, and altogether extract three times, the mass ratio of each Soybean Leaves and alcoholic solution is 1:3, each extraction 0.5 hour; The model of macroporous resin is D101; The flow velocity that extracting solution flows through macroporous resin is 3mL/min; During eluting, selective body volume concentrations is the alcoholic solution of 50% is eluant, and elution flow rate is 3mL/min.
Claims (5)
1. from Soybean Leaves, extract a method for total flavones, it is characterized in that: by Soybean Leaves after alcoholic solution extracts, with macroporous resin adsorption by extracting solution through upper prop, eluting, collection eluent, eluent is concentrated, obtain product after drying.
2. method according to claim 1, is characterized in that: described Soybean Leaves volumetric concentration is the alcoholic solution extraction of 30% ~ 80%, and altogether extract three times, the mass ratio of each Soybean Leaves and alcoholic solution is 1:3 ~ 10, extracts 0.1 ~ 1 hour at every turn.
3. method according to claim 1, is characterized in that: described macroporous resin is any one in AB-8, HZ818, HPD-100 and D101.
4. method according to claim 1, is characterized in that: the flow velocity that described extracting solution flows through macroporous resin is 1 ~ 3mL/min.
5. method according to claim 1, is characterized in that: during described eluting, selective body volume concentrations is the alcoholic solution of 50% ~ 95% is eluant, and elution flow rate is 1 ~ 4mL/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410710444.4A CN104490987A (en) | 2014-11-28 | 2014-11-28 | Method for extracting general flavone from soybean leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410710444.4A CN104490987A (en) | 2014-11-28 | 2014-11-28 | Method for extracting general flavone from soybean leaves |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104490987A true CN104490987A (en) | 2015-04-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410710444.4A Pending CN104490987A (en) | 2014-11-28 | 2014-11-28 | Method for extracting general flavone from soybean leaves |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104490987A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009109402A2 (en) * | 2008-03-07 | 2009-09-11 | S.W. Patentverwertungs Limited | Composition and uses for influencing hair growth |
| CN103751260A (en) * | 2014-01-10 | 2014-04-30 | 山东省高唐蓝山集团总公司 | Method for extracting soy isoflavone and soyasaponin from soy hilum |
-
2014
- 2014-11-28 CN CN201410710444.4A patent/CN104490987A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009109402A2 (en) * | 2008-03-07 | 2009-09-11 | S.W. Patentverwertungs Limited | Composition and uses for influencing hair growth |
| CN103751260A (en) * | 2014-01-10 | 2014-04-30 | 山东省高唐蓝山集团总公司 | Method for extracting soy isoflavone and soyasaponin from soy hilum |
Non-Patent Citations (2)
| Title |
|---|
| 戴胜军等: "大豆叶化学成分研究", 《中草药》 * |
| 杜成林等: "大豆茎叶化学成分及其生理活性的研究", 《第六届全国药用植物和植物药学术研讨会论文集》 * |
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| WD01 | Invention patent application deemed withdrawn after publication | ||
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Application publication date: 20150408 |