CN104489702A - Anti-oxidation and anti-fatigue health product and preparation method thereof - Google Patents

Abstract

The invention discloses a health care product with anti-oxidation and anti-fatigue effects and a preparation method thereof, comprising the following components in parts by weight: 25-75 parts of male silkworm moth protein powder, 50-350 parts of maca powder, 10-350 parts of maca powder 30 parts of Cordyceps extract, 5-20 parts of mulberry fruit extract, 10-25 parts of Lycium barbarum extract; Maca powder prepared by the present invention is soluble Maca powder, and male silkworm moth protein powder is superfine male silkworm moth protein powder. The health product prepared by the soluble maca powder and the superfine male silk moth protein powder has anti-fatigue and anti-oxidation effects; and the prepared maca powder, male silkworm protein powder, Cordyceps extract, mulberry fruit extract, wolfberry The extracts are all natural and safe active ingredients, which are easily absorbed by the body.

Description

A health product with anti-oxidation and anti-fatigue effects and preparation method thereof

technical field

The invention relates to the field of health food, in particular to a health product with anti-oxidation and anti-fatigue effects and a preparation method thereof.

Background technique

The application of male silkworm moth in traditional Chinese medicine has been recorded since ancient times. As a medicinal and edible insect, it not only contains a large amount of nutrients, but also has extremely rich physiologically active substances. The crude protein content of male silkworm moth is higher than that of eggs and pork. It contains not only a complete range of amino acids, but also an appropriate proportion. The male silkworm moth also contains rich biologically active substances such as brain hormones, molting hormones and juvenile hormones. It has been recognized by the Ministry of Health as "animal health food raw materials with specific health functions ". According to modern scientific research, the silkworm male moth has male hormone-like effects, as well as anti-aging, anti-fatigue, hypoglycemic, liver and liver protection effects, and also has immunological effects.

Maca is an annual herb belonging to the genus Brassicaceae, native to the Andes in Peru at an altitude of 3500-4500 m. Traditionally used as food and herbal medicine to enhance energy, fight fatigue, improve sexual function, improve fertility, and treat menopausal syndrome in women, etc., without any toxic and side effects, it has the reputation of "Peruvian ginseng" and "South American ginseng" among the local people .

Some data show that excessive fatigue is the main cause of sub-health, and eliminating fatigue is an effective way to prevent sub-health. Simple nutritional supplements can no longer meet people's needs. People hope to obtain both nutrients while supplementing nutrients. It can resist fatigue, resist self-oxidation, enhance immunity and many other effects. Therefore, it will have a good market prospect to develop a health product that can simultaneously have anti-oxidation and anti-fatigue effects.

Contents of the invention

In order to overcome the shortcomings and deficiencies of the prior art, the primary purpose of the present invention is to provide a health product with anti-oxidation and anti-fatigue effects.

Another object of the present invention is to provide a preparation method of the above-mentioned health product with anti-oxidation and anti-fatigue effects.

The present invention adopts following technical scheme to realize the purpose of the present invention:

A health product with anti-oxidation and anti-fatigue effects, comprising the following components in parts by weight:

Male silkworm moth protein powder 25~75 servings,

Maca powder 50~350 parts,

Cordyceps extract 10~30 parts,

Mulberry extract 5~20 parts,

Lycium barbarum extract 10~25 parts.

Preferably, a health product with anti-oxidation and anti-fatigue effects includes the following components in parts by weight:

Male silkworm moth protein powder 55 servings,

Maca powder 180 parts,

Cordyceps extract 10 parts,

Mulberry Extract 15 parts,

Lycium barbarum extract 20 servings.

A preparation method of the above-mentioned health product with anti-oxidation and anti-fatigue effects, comprising the steps of:

a) Preparation of male silk moth protein powder: put the male silkworm moth in a vacuum microwave dryer to dry, then pulverize at low temperature, and then undergo supercritical carbon dioxide extraction to remove the lipids in the male silkworm moth, and then use an airflow ultrafine pulverizer to pulverize to 200 mesh Above, obtain male silkworm moth protein powder;

b) Preparation of maca powder: wash the maca root and dry it in a vacuum microwave dryer, slice it, crush it at low temperature, pass through a 60-mesh sieve, extract it with ultrasonic waves, concentrate and dry it in vacuum, and obtain maca powder;

c) Preparation of Cordyceps extract: Soak and decoct Cordyceps in water, add 5 to 10 times the amount of water, decoct for 1 to 3 hours, and decoct for 1 to 3 times; filter and combine the filtrates, concentrate at 50°C Cordyceps extract extract with a relative density of 1.2~1.3;

d) Preparation of mulberry extract: Soak and decoct mulberries in water, add 5 to 10 times the amount of water, decoct for 1 to 3 hours, and decoct for 1 to 3 times; filter and combine the filtrates, concentrate at 50°C It is the extract of mulberry fruit extract with a relative density of 1.2~1.3;

e) Preparation of Lycium barbarum extract: Soak Lycium barbarum in water, decoct, add 5-10 times of water, decoct for 1-3 hours, and decoct for 1-3 times; filter and combine filtrates, concentrate at 50°C Lycium barbarum extract extract with a relative density of 1.2~1.3;

f) The active ingredients prepared in steps a)~e) are added with various conventional excipients such as disintegrants, lubricants, and adhesives required for the preparation of different dosage forms, and then prepared by conventional traditional Chinese medicine preparation methods such as Common dosage forms of pills, tablets, capsules, and oral liquids.

Wherein, the drying temperature of the vacuum microwave drying in the step a) and the step b) is 30-60°C, and the moisture content after drying is 10wt%. Wherein, the specific technological conditions of the low-temperature crushing in the step a) are that silkworm chrysalis are pre-cooled at -60~-30°C for 1 hour, the crushing temperature is -60°C, and the crushing time is 2~3 minutes. .

Wherein, the specific process conditions of supercritical carbon dioxide extraction in the step a) are pressure 10-20MPa, temperature 30-50°C, CO2 flow rate 10-15kg/h, and extraction time 60min.

Wherein, the specific process conditions of the low-temperature crushing in the step b) are that maca is precooled at -80~-60°C for 1~2h, the crushing temperature is -80°C, and the crushing time is 3 minutes.

Wherein, the specific process conditions of the ultrasonic extraction in the step b) are that the ultrasonic frequency is 20-30 KHz, and the extraction time is 20-30 min.

Wherein, the specific process conditions of vacuum concentration and drying in the step b) are a temperature of 40-60° C. and a vacuum degree of 0.095 MPa.

Compared with the prior art, the present invention has the following beneficial effects:

1) The maca powder prepared by the present invention is easily soluble maca powder, and the male silk moth protein powder is ultrafine male silkworm protein powder, and the health care product prepared from the easily soluble maca powder and ultrafine male silkworm protein powder has the following properties: Anti-fatigue and antioxidant effects.

2) The male silkworm moth protein powder prepared by the present invention, after supercritical carbon dioxide extraction to remove lipids, has a protein yield twice that of the non-fat removal, which saves raw materials and reduces costs.

3) The maca powder, male silkworm moth protein powder, cordyceps extract, mulberry extract, and wolfberry extract prepared by the present invention are all natural and safe active ingredients, which are easily absorbed by the human body.

Detailed ways

The present invention will be further described below through specific embodiments. The following examples are preferred implementation forms of the present invention, but the implementation manners of the present invention are not limited by the following examples.

Example 1

A health product with antioxidant and anti-fatigue effects, comprising the following components in parts by weight: 55 parts of male silk moth protein powder, 180 parts of maca powder, 10 parts of Cordyceps extract, 15 parts of mulberry extract, 20 parts of Lycium barbarum extract;

A preparation method comprising the above-mentioned health product with anti-oxidation and anti-fatigue effects, comprising the steps of:

a) Preparation of male silk moth protein powder: put the male silkworm moth in a vacuum microwave dryer to dry, then pulverize at low temperature, and then undergo supercritical carbon dioxide extraction to remove the lipids in the male silkworm moth, concentrate and dry in vacuum, and then use an airflow ultrafine pulverizer Pulverize to more than 200 mesh to obtain male silkworm moth protein powder;

b) Preparation of maca powder: wash the maca root and dry it in a vacuum microwave dryer, slice it, crush it at low temperature, pass through a 60-mesh sieve, extract it with ultrasonic waves, concentrate and dry it in vacuum, and obtain maca powder;

c) Preparation of Cordyceps extract: Soak and decoct Cordyceps in water, add 5 to 10 times the amount of water, decoct for 1 to 3 hours, and decoct for 1 to 3 times; filter and combine the filtrates, concentrate at 50°C Cordyceps extract extract with a relative density of 1.2~1.3;

d) Preparation of mulberry extract: Soak and decoct mulberries in water, add 5 to 10 times the amount of water, decoct for 1 to 3 hours, and decoct for 1 to 3 times; filter and combine the filtrates, concentrate at 50°C It is the extract of mulberry fruit extract with a relative density of 1.2~1.3;

e) Preparation of Lycium barbarum extract: Soak Lycium barbarum in water, decoct, add 5-10 times of water, decoct for 1-3 hours, and decoct for 1-3 times; filter and combine filtrates, concentrate at 50°C Lycium barbarum extract extract with a relative density of 1.2~1.3;

f) The active ingredients prepared in steps a)~e) are added with various conventional excipients required for the preparation of capsules, and then prepared into capsules by conventional traditional Chinese medicine preparation methods.

Example 2

A health product with antioxidant and anti-fatigue effects, comprising the following components in parts by weight: 25 parts of male silk moth protein powder, 50 parts of maca powder, 10 parts of Cordyceps extract, 5 parts of mulberry fruit extract, 10 parts of Lycium barbarum extract;

A preparation method comprising the above-mentioned health product with anti-oxidation and anti-fatigue effects, comprising the steps of:

a) Preparation of male silk moth protein powder: put the male silkworm moth in a vacuum microwave dryer to dry, then pulverize at low temperature, and then undergo supercritical carbon dioxide extraction to remove the lipids in the male silkworm moth, concentrate and dry in vacuum, and then use an airflow ultrafine pulverizer Pulverize to more than 200 mesh to obtain male silkworm moth protein powder;

b) Preparation of maca powder: wash the maca root and dry it in a vacuum microwave dryer, slice it, crush it at low temperature, pass through a 60-mesh sieve, extract it with ultrasonic waves, concentrate and dry it in vacuum, and obtain maca powder;

c) Preparation of Cordyceps extract: Soak and decoct Cordyceps in water, add 5 to 10 times the amount of water, decoct for 1 to 3 hours, and decoct for 1 to 3 times; filter and combine the filtrates, concentrate at 50°C Cordyceps extract extract with a relative density of 1.2~1.3;

d) Preparation of mulberry extract: Soak and decoct mulberries in water, add 5 to 10 times the amount of water, decoct for 1 to 3 hours, and decoct for 1 to 3 times; filter and combine the filtrates, concentrate at 50°C It is the extract of mulberry fruit extract with a relative density of 1.2~1.3;

e) Preparation of Lycium barbarum extract: Soak Lycium barbarum in water, decoct, add 5-10 times of water, decoct for 1-3 hours, and decoct for 1-3 times; filter and combine filtrates, concentrate at 50°C Lycium barbarum extract extract with a relative density of 1.2~1.3;

f) The active ingredients prepared in steps a)~e) are added with various conventional excipients required for tablet preparation, and then prepared into tablets by conventional traditional Chinese medicine preparation methods.

Example 3

A health product with antioxidant and anti-fatigue effects, comprising the following components in parts by weight: 75 parts of male silk moth protein powder, 350 parts of maca powder, 30 parts of Cordyceps extract, 20 parts of mulberry extract, 25 parts of Lycium barbarum extract;

A preparation method comprising the above-mentioned health product with anti-oxidation and anti-fatigue effects, comprising the steps of:

a) Preparation of male silk moth protein powder: put the male silkworm moth in a vacuum microwave dryer to dry, then pulverize at low temperature, and then undergo supercritical carbon dioxide extraction to remove the lipids in the male silkworm moth, concentrate and dry in vacuum, and then use an airflow ultrafine pulverizer Pulverize to more than 200 mesh to obtain male silkworm moth protein powder;

b) Preparation of maca powder: wash the maca root and dry it in a vacuum microwave dryer, slice it, crush it at low temperature, pass through a 60-mesh sieve, extract it with ultrasonic waves, concentrate and dry it in vacuum, and obtain maca powder;

c) Preparation of Cordyceps extract: Soak and decoct Cordyceps in water, add 5 to 10 times the amount of water, decoct for 1 to 3 hours, and decoct for 1 to 3 times; filter and combine the filtrates, concentrate at 50°C Cordyceps extract extract with a relative density of 1.2~1.3;

d) Preparation of mulberry extract: Soak and decoct mulberries in water, add 5 to 10 times the amount of water, decoct for 1 to 3 hours, and decoct for 1 to 3 times; filter and combine the filtrates, concentrate at 50°C It is the extract of mulberry fruit extract with a relative density of 1.2~1.3;

e) Preparation of Lycium barbarum extract: Soak Lycium barbarum in water, decoct, add 5-10 times of water, decoct for 1-3 hours, and decoct for 1-3 times; filter and combine filtrates, concentrate at 50°C Lycium barbarum extract extract with a relative density of 1.2~1.3;

f) The active ingredients prepared in steps a)~e) are added with various conventional excipients required for the preparation of oral liquids, and then prepared into oral liquids by conventional traditional Chinese medicine preparation methods.

Pharmacodynamics Experiment 1

Pharmacodynamic experiments show that the health care products with anti-oxidation and anti-fatigue effects of the present invention are positive in the weight-bearing swimming test, and the results of the three biochemical indicators of serum urea nitrogen, liver glycogen, and blood lactic acid are also all positive. Positive, it shows that the health product with anti-oxidation and anti-fatigue effects of the present invention has the effect of anti-fatigue function.

Experimental materials and methods

1. 1 Experimental samples

The health product with antioxidant and anti-fatigue effects obtained in Example 1 was selected as the experimental sample.

1.2 Experimental animals and breeding

KM male mice with a body weight of 18-22 g were selected.

1.3 Experimental method

The clean-grade male Kunming mice were randomly divided into four groups, and the dosage of the mice was 10 times larger than the recommended dose of the human body, and two dose groups were set up and down, namely, the high, medium and low dose groups of the test substance (2.00 g/ kg.bw.d, 0.50 g/kg.bw.d, 0.10 g/kg.bw.d), and a blank control group was set up to carry out weight-bearing swimming test, serum urea nitrogen determination, blood lactic acid determination, hemoglobin determination, lactic acid Dehydrogenase assay experiment and liver glycogen assay experiment. The mice were kept under normal light, drinking water, and kept at 25°C. Oral gavage once a day, the blank group was gavaged with normal saline, the amount of gavage was 1% of the mice's body weight, and all indicators were measured after 30 consecutive days.

1.3.1 Weight-bearing swimming test

30 minutes after the last administration of the test drug, the root of the tail of the rat was loaded with a lead wire of 5% body weight, and the mouse was placed in a swimming box (40cm×60cm×36cm) for swimming. The water depth was not less than 30 cm, and the water temperature was (25 ± 1) , record the time from the start of swimming to the death of the mice, that is, the weight-bearing swimming time of the mice.

* indicates significant difference compared with the control group (P<0.05).

1.3.2 Effects on serum urea nitrogen and liver glycogen content in mice

30 minutes after the last administration of the test substance, the mice were placed in the swimming box and swam without weight for 90 minutes, the water temperature was (30 ± 1) ℃, and after a 60-minute rest, the eyeballs were removed to collect blood, and the blood samples were centrifuged at 3000 rpm for 5 minutes to separate the serum . Blood urea nitrogen (BUN) was measured with a blood urea nitrogen (BUN) assay kit.

30 minutes after the last administration of the test substance, the animals were sacrificed by cervical dislocation. The liver was taken out, rinsed with normal saline and blotted dry with filter paper, and liver glycogen was measured with a glycogen assay kit.

* indicates a significant difference compared with the control group (P<0.05).

1.3.3 Effects on blood lactic acid content in mice

30 minutes after the last administration of the test substance, the mice were placed in the swimming box to swim without weight for 10 minutes, the water temperature was (30 ± 1) ℃, and they rested for 20 minutes before and after swimming, and then the blood was collected from the eyeballs. The blood samples were Serum was separated by centrifugation at 3000 rpm for 5 min. Blood lactate was measured with a lactate (LD) assay kit.

Calculate the area under the blood lactate curve:

5× (blood lactic acid value before swimming + 3× blood lactic acid value at 0 min after swimming + 2× blood lactic acid value after 20 min of rest after swimming).

* indicates significant difference compared with the control group (P<0.05).

Experimental results

The health product with anti-oxidation and anti-fatigue effects of the present invention has positive results in the weight-bearing swimming test, and the results of the three biochemical indicators of serum urea nitrogen, liver glycogen, and blood lactic acid are also positive, indicating that the health products of the present invention Health products with anti-oxidation and anti-fatigue effects have anti-fatigue functions.

Pharmacodynamics Experiment II

Pharmacodynamic experiments show that the health care product with anti-oxidation and anti-fatigue effects of the present invention, the antioxidant enzyme activity and lipid peroxidation content indicators are all positive, indicating that the health care product with anti-oxidation and anti-fatigue effects of the present invention The product has the function of anti-oxidation.

Experimental materials and methods

1. 1 Experimental samples

The health product with antioxidant and anti-fatigue effects obtained in Example 2 was selected as the experimental sample.

1. 2 Experimental objects

12-month-old male rats were used.

1.3 Experimental method

The rats were randomly divided into four groups, and the intragastric dose was 10 times the recommended dose for the human body. Two dose groups were set up and down, that is, the high, medium and low dose groups of the test substance (2.00 g/kg.bw.d, 0.50 g/kg.bw.d, 0.10 g/kg.bw.d), and a blank control group with 12 rats in each group. Rats were kept under normal light, drinking water, and kept at 25°C. The blank group was intragastrically administered once a day with the same volume of normal saline for 30 consecutive days. After the experiment, the eyeballs were removed to collect blood to prepare serum and determine the content of lipid peroxide malondialdehyde (MDA). Barbituric acid (TBA) fluorescence spectrophotometry; the activity of antioxidant enzyme superoxide dismutase (SOD) was measured by hydroxylamine colorimetry.

1.3.1 Analysis of experimental results

The experimental data were analyzed by variance analysis using SPSS statistical software.

1.3.2 Determination of the content of malondialdehyde (MDA), the degradation product of lipid peroxidation in serum

* indicates significant difference compared with the control group (P<0.05).

1.3.3 Determination results of superoxide dismutase (SOD) activity in serum

* indicates a significant difference compared with the control group (P<0.05).

Experimental results

Both the antioxidant enzyme activity and the lipid peroxidation content index are positive, indicating that the health product with anti-oxidation and anti-fatigue effects of the present invention has an anti-oxidation function.

Claims (9)

1. there are anti-oxidant and health products that are antifatigue effect, it is characterized in that, by weight, comprise following component:

Male silk moth albumen powder 25 ~ 75 parts,

Pueraria root powder 50 ~ 350 parts,

Cordyceps 10 ~ 30 parts,

Fructus Mori extract 5 ~ 20 parts,

Wolfberry fruit extract 10 ~ 25 parts.

2. according to claim 1 have anti-oxidant and health products that are antifatigue effect, it is characterized in that, by weight, comprise following component:

Male silk moth albumen powder 55 parts,

Pueraria root powder 180 parts,

Cordyceps 10 parts,

Fructus Mori extract 15 parts,

Wolfberry fruit extract 20 parts.

3. comprise the preparation method with the health products of anti-oxidant and antifatigue effect described in claim 1 or 2, it is characterized in that, comprise the steps:

A) preparation of male silk moth albumen powder: male silk moth is placed in vacuum microwave drier dry, then low-temperature grinding, then through supercritical carbon dioxide extracting, the lipid in male silk moth is removed, then be crushed to more than 200 orders with air-flow micronizer, obtain male silk moth albumen powder;

B) preparation of pueraria root powder: cleaned by agate coffee root and be placed on drying in vacuum microwave drier, low-temperature grinding after section, cross 60 mesh sieves, then extract through ultrasonic wave, Vacuum Concentration drying, obtains pueraria root powder;

C) preparation of Cordyceps: Chinese caterpillar fungus is soaked in water, decocts, amount of water is 5 ~ 10 times, and decocting time is 1 ~ 3 hour, and decocting number of times is 1 ~ 3 time; Merging filtrate after filtering, the Cordyceps medicinal extract of 50 DEG C of simmer down to relative densities 1.2 ~ 1.3;

D) preparation of Fructus Mori extract: mulberry fruit is soaked in water, decocts, amount of water is 5 ~ 10 times, and decocting time is 1 ~ 3 hour, and decocting number of times is 1 ~ 3 time; Merging filtrate after filtering, the Fructus Mori extract medicinal extract of 50 DEG C of simmer down to relative densities 1.2 ~ 1.3;

E) preparation of wolfberry fruit extract: matrimony vine is soaked in water, decocts, amount of water is 5 ~ 10 times, and decocting time is 1 ~ 3 hour, and decocting number of times is 1 ~ 3 time; Merging filtrate after filtering, the wolfberry fruit extract medicinal extract of 50 DEG C of simmer down to relative densities 1.2 ~ 1.3;

F) by active component that step a) ~ step e) prepares, add the various customary adjuvant as disintegrant, lubricant, adhesive required when preparing different dosage form, then be prepared into the common formulations as pill, tablet, capsule, oral liquid with the method for Chinese medicinal of routine.

4. the preparation method with the health products of anti-oxidant and antifatigue effect according to claim 3, is characterized in that, the baking temperature of the vacuum microwave drying in described step a) and step b) is 30 ~ 60 DEG C, and dried moisture is 10wt%.

5. according to claim 3 have preparation method that the is anti-oxidant and health products of antifatigue effect, it is characterized in that, in described step a) the concrete technology condition of low-temperature grinding be silkworm chrysalis at-60 ~-30 DEG C of precooling 1h, pulverize temperature be-60 DEG C, grinding time is 2 ~ 3min.

6. according to claim 3 have preparation method that the is anti-oxidant and health products of antifatigue effect, and it is characterized in that, in described step a), the concrete technology condition of supercritical carbon dioxide extracting is pressure 10 ~ 20MPa, temperature 30 ~ 50 DEG C, CO ₂flow is 10 ~ 15kg/h, and extraction time is 60min.

7. according to claim 3 have preparation method that the is anti-oxidant and health products of antifatigue effect, it is characterized in that, in described step b) the concrete technology condition of low-temperature grinding be agate coffee at-80 ~-60 DEG C of precooling 1 ~ 2h, pulverize temperature be-80 DEG C, grinding time is 3min.

8. according to claim 3 have preparation method that the is anti-oxidant and health products of antifatigue effect, and it is characterized in that, the concrete technology condition that in described step b), ultrasonic wave extracts is supersonic frequency 20 ~ 30KHz, and extraction time is 20 ~ 30min.

9. according to claim 3 have preparation method that the is anti-oxidant and health products of antifatigue effect, and it is characterized in that, in described step b), the concrete technology condition of cryogenic vacuum concentrate drying is temperature 40 ~ 60 DEG C, and vacuum is 0.095MPa.