CN104459117A - Carbon nano-tube procalcitonin detection kit and preparation method thereof - Google Patents
Carbon nano-tube procalcitonin detection kit and preparation method thereof Download PDFInfo
- Publication number
- CN104459117A CN104459117A CN201410740721.6A CN201410740721A CN104459117A CN 104459117 A CN104459117 A CN 104459117A CN 201410740721 A CN201410740721 A CN 201410740721A CN 104459117 A CN104459117 A CN 104459117A
- Authority
- CN
- China
- Prior art keywords
- tube
- carbon nano
- procalcitonin
- colloidal gold
- pad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 96
- 239000002041 carbon nanotube Substances 0.000 title claims abstract description 90
- 229910021393 carbon nanotube Inorganic materials 0.000 title claims abstract description 90
- 108010048233 Procalcitonin Proteins 0.000 title claims abstract description 48
- CWCXERYKLSEGEZ-KDKHKZEGSA-N procalcitonin Chemical compound C([C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)NCC(O)=O)[C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCSC)NC(=O)[C@H]1NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@@H](N)CSSC1)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 CWCXERYKLSEGEZ-KDKHKZEGSA-N 0.000 title claims abstract description 48
- 238000001514 detection method Methods 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 66
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 12
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 12
- 238000012360 testing method Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000002131 composite material Substances 0.000 claims description 33
- 239000010931 gold Substances 0.000 claims description 22
- 229910052737 gold Inorganic materials 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 10
- 239000006185 dispersion Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000002109 single walled nanotube Substances 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 241001494479 Pecora Species 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 239000002274 desiccant Substances 0.000 claims description 3
- 230000033444 hydroxylation Effects 0.000 claims description 3
- 238000005805 hydroxylation reaction Methods 0.000 claims description 3
- 239000002048 multi walled nanotube Substances 0.000 claims description 3
- 238000001338 self-assembly Methods 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- 230000000274 adsorptive effect Effects 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 13
- 238000005516 engineering process Methods 0.000 abstract description 4
- 239000012528 membrane Substances 0.000 abstract description 4
- 238000002372 labelling Methods 0.000 abstract description 3
- 208000035143 Bacterial infection Diseases 0.000 abstract description 2
- 208000022362 bacterial infectious disease Diseases 0.000 abstract description 2
- 238000003908 quality control method Methods 0.000 abstract description 2
- 230000002745 absorbent Effects 0.000 abstract 1
- 239000002250 absorbent Substances 0.000 abstract 1
- 239000000427 antigen Substances 0.000 description 8
- 102000036639 antigens Human genes 0.000 description 8
- 108091007433 antigens Proteins 0.000 description 8
- 239000000084 colloidal system Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 239000002245 particle Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 206010036790 Productive cough Diseases 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 210000003802 sputum Anatomy 0.000 description 3
- 208000024794 sputum Diseases 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000005784 autoimmunity Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nanotechnology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a carbon nano-tube procalcitonin detection kit and a preparation method thereof. A test paper base plate is sequentially adhered with a sample pad, a carbon nano-tube colloidal gold pad, a nitrocellulose membrane and a water absorbent pad. The carbon nano-tube procalcitonin detection kit is characterized in that the carbon nano-tube colloidal gold pad is adhered with a carbon nano-tube colloidal gold compound labeled procalcitonin monoclonal antibody 1, and the nitrocellulose membrane is coated with a detection line and a quality control line of a procalcitonin monoclonal antibody 2. According to the kit disclosed by the invention, procalcitonin in a sample is detected by adopting a carbon nano-tube colloidal gold labelling technology so as to judge whether bacterial infection occurs. The carbon nano-tube procalcitonin detection kit disclosed by the invention has the characteristics of simplicity in operation, rapid reaction, high sensitivity, strong specificity and the like, and is suitable for field detection and self-detection.
Description
Technical field
The present invention relates to a kind of external diagnosis reagent case, specifically, relate to a kind of carbon nano-tube Procalcitonin detection kit and preparation method thereof, be applicable to the Procalcitonin in clinical examination sample.
Background technology
Colloidal gold immunochromatographimethod technology is widely used in multiple fields such as disease markers detection, food inspection, scientific research, selected trace labelling material is generally collaurum, latex particle etc., chromatographic material is generally cellulose membrane, comprise nitrocellulose filter, cellulose acetate membrane, cellulose mixture film etc., wherein the most frequently used is nitrocellulose filter.Under current technical conditions, the tracer materials such as collaurum and nitrocellulose filter is used to be difficult to improve detection sensitivity again as carrier.
The not high reason of sensitivity is a lot, comprise raw material, technique etc., but the underlying cause is the limitation of collaurum method itself, improves sensitivity again by traditional collaurum method is very difficult.Now general resolving ideas increases amplification system in collaurum system, such as adopts biotin-avidin amplification system.
Summary of the invention
The invention provides a kind of carbon nano-tube Procalcitonin detection kit and preparation method thereof, its technical matters to be solved is: provide a kind of carbon nano-tube colloidal gold composite, as trace labelling material, for marking Procalcitonin antibody, improve the sensitivity detected.
the present invention adopts following technical scheme to realize:
A kind of carbon nano-tube Procalcitonin detection kit, is characterized in that: this kit is with carbon nano-tube colloidal gold composite mark Procalcitonin antibody.
Test paper base plate is pasted with successively sample pad, carbon nano-tube colloidal gold composite pad, nitrocellulose filter and adsorptive pads; Described carbon nano-tube colloidal gold composite pad is attached with the Procalcitonin monoclonal antibody 1 of carbon nano-tube colloidal gold composite mark, described nitrocellulose filter is coated with detection line and the nature controlling line of Procalcitonin monoclonal antibody 2.
Carbon nano-tube 4 used is the one in Single Walled Carbon Nanotube, multi-walled carbon nano-tubes, hydroxylation carbon nano-tube, carboxylic carbon nano-tube, water-soluble carbon nanometer tube.
Carbon nano-tube 4 and collaurum 3 form carbon nano-tube colloidal gold composite by self assembly.
Described carbon nano-tube colloidal gold composite, wherein carbon nano-tube 4 accounts for colloidal gold composite gross mass is 0.1 ~ 20.0%.
A kind of preparation method of carbon nano-tube Procalcitonin detection kit as above, it is characterized in that: the Procalcitonin monoclonal antibody 1 described carbon nano-tube colloidal gold composite pad being attached with carbon nano-tube colloidal gold composite mark, described nitrocellulose filter is coated with detection line and the nature controlling line of Procalcitonin monoclonal antibody 2; Concrete steps are as follows:
(1) preparation of carbon nano tube dispersion liquid: get the surfactant adding 0.5 ~ 5.0g in 100ml pure water, after stirring, adds 10 ~ 1000mg carbon nano-tube 4, and the ultrasonic 2 ~ 10min of ultrasonic cell disruptor, obtains carbon nanotube dispersed solution;
(2) preparation of carbon nano-tube colloidal gold composite: take the Procalcitonin antibody that collaurum 3 has marked, adds the above-mentioned carbon nano tube dispersion liquid of mass ratio 0.1 ~ 30.0%, stirs 5 ~ 30 minutes; Centrifugal 30 ~ 60min;
(3) preparation of carbon nano-tube colloidal gold composite pad: abandoning supernatant, then in centrifuge tube, add gold mark dilution, after mixing, by above-mentioned solution spraying on the gold mark pad processed, drying for standby;
(4) detection line, nature controlling line draw film: be affixed on base plate by NC film, stand-by; The Procalcitonin monoclonal antibody 2 of preparation 0.5mg/ml; Compound concentration is 1.0mg/ml sheep anti-mouse antibody; Respectively on ready NC film before, draw film with 1.0 μ l/cm by point film instrument again, be respectively T, C line, drying at room temperature 2 hours;
(5) assemble: more dried colloidal gold pad and thieving paper, sample pad, the base plate that posts NC film are assembled, then the test strips of 4.0mm is cut into by automatic cutting machine, again test strips and cartridge are carried out pressure shell, then kit and drying agent, suction pipe are assembled and sealed.
Advantage and effect: it is generally acknowledged are 10 when the colloid gold particle of every square millimeter gathers
7time individual, detection line could obviously develop the color, and just can improve detection sensitivity by the accumulated amount increasing collaurum.In line with this thinking, adopt carbon nano-tube and collaurum to combine the method forming compound and improve detection sensitivity.
Carbon nano-tube is a kind of novel nano-material, lightweight, and hexagonal structure connects perfect, has many abnormal mechanics, electricity and chemical property.Carbon nano-tube is existing many research in immunoassay, mainly concentrates on immunosensor field, can increase substantially detection sensitivity.Carbon nano-tube is divided into Single Walled Carbon Nanotube, multi-walled carbon nano-tubes, hydroxylation carbon nano-tube, carboxylic carbon nano-tube, also can obtain water-soluble carbon nanometer tube after treatment, has good adsorption separation performance, and the carbon nano-tube of functionalization more easily and protein bound.The compound labelled protein of carbon nano-tube and collaurum, can improve the sensitivity detected.
Procalcitonin (PCT) is a kind of protein, and when serious bacterial, fungi, parasitic infection and pyemia and MOFE, its level in blood plasma raises.When autoimmunity, allergy and virus infections, PCT can not raise.
In respiratory tract bacterial infection patient, the Procalcitonin Concentrations in sputum and pharyngeal juice all can raise, and its elevated levels, generally higher than blood, so detect the Procalcitonin content in sputum and throat swab, can diagnose the type of respiratory tract infection.
accompanying drawing illustrates:
Fig. 1 is carbon nano-tube colloidal gold composite micromechanism schematic diagram of the present invention.
Mark in figure: collaurum 3, carbon nano-tube 4.
embodiment:
A carbon nano-tube can adsorb multiple colloid gold particle; as long as wherein have one or several antibody and determined antigen to form sandwich complex on detection line; just can other colloid gold particles in carbon nano-tube be accumulated on detection line simultaneously, increase colored intensity, thus improve detection sensitivity.
Fig. 1 is carbon nano-tube colloidal gold composite schematic diagram, and collaurum and carbon nano-tube can form compound by self assembly, and a carbon nano-tube can adsorb multiple gold grain.In general colloidal gold method, a gold grain marked and a determined antigen combine, and on detection line, form centre-fills colour developing.After adopting carbon nano-tube colloidal gold composite, if a gold grain in carbon nano-tube and determined antigen combine, take detection line to together with the gold grain that so just other in carbon nano-tube can not combined with determined antigen and stop colour developing, increase the accumulated amount of gold grain at detection line, color developing effect is more obvious.In most cases, determined antigen will be less than the antibody of gold mark, and antibody is often excessive in other words, especially all the more so when detecting trace antigen, such as detects trace P CT.In this case, a large amount of gold grains do not combined with determined antigen does not stop at detection line, and direct chromatography is gone over, and colloidal gold method sensitivity that Here it is is difficult to the basic reason improved.By adopting carbon nano-tube colloidal gold composite technology, the colloid gold particle in conjunction with determined antigen just can be allowed to stop on detection line, thus improve detection sensitivity.
A kind of carbon nano-tube Procalcitonin detection kit and preparation method thereof, described carbon nano-tube colloidal gold composite pad is attached with the Procalcitonin monoclonal antibody 1 of carbon nano-tube colloidal gold composite mark, described nitrocellulose filter is coated with detection line and the nature controlling line of Procalcitonin monoclonal antibody 2.
The preparation of carbon nano tube dispersion liquid: get the surfactant adding 0.5 ~ 5.0g in 100ml ultrapure water, after stirring, adds 10 ~ 1000mg carbon nano-tube, and the ultrasonic 2 ~ 10min of ultrasonic cell disruptor, obtains carbon nanotube dispersed solution;
The preparation of carbon nano-tube colloidal gold composite: take the Procalcitonin antibody that colloid gold label is good, adds the above-mentioned carbon nano tube dispersion liquid of mass ratio 0.1 ~ 30.0%, stirs 5 ~ 30 minutes.Centrifugal 30 ~ 60min.
The preparation of carbon nano-tube colloidal gold composite pad: abandoning supernatant, then in centrifuge tube, add gold mark dilution, after mixing, by above-mentioned solution spraying on the gold mark pad processed, drying for standby.
Below in conjunction with embodiment, the present invention is further elaborated, but protection scope of the present invention not limit by embodiment.
Embodiment
1, the preparation of carbon nano tube dispersion liquid: get the Tween-20 adding 2.0g in 100ml pure water, after stirring, adds 20mg Single Walled Carbon Nanotube, and the ultrasonic 2min of ultrasonic cell disruptor, obtains the carbon nano-tube solution of 0.2mg/ml;
2, colloid gold label Procalcitonin antibody: get the colloidal gold solution 100ml prepared and add in beaker, measured by acidometer, with the solution of potassium carbonate adjust pH of 0.2M for 8.0, adds the Procalcitonin monoclonal antibody 1, magnetic agitation 30min of 0.5mg.
3, the preparation of carbon nano-tube colloidal gold composite: take the Procalcitonin antibody that colloid gold label is good, adds the above-mentioned carbon nano tube dispersion liquid of 0.1ml, stirs 30 minutes.Low-temperature centrifugation 30min.
4, the preparation of carbon nano-tube colloidal gold composite pad: abandoning supernatant, then in centrifuge tube, add gold mark dilution, after mixing, by above-mentioned solution spraying on gold mark pad, drying for standby.
5, detection line, nature controlling line draw film: be affixed on base plate by NC film, stand-by.The Procalcitonin monoclonal antibody 2 of preparation 0.5mg/ml; Compound concentration is 1.0mg/ml sheep anti-mouse antibody; Respectively on ready NC film before, draw film with 1.0 μ l/cm by point film instrument again, be respectively T, C line, drying at room temperature 2 hours.
6, assemble: more dried colloidal gold pad and thieving paper, sample pad, the base plate that posts NC film are assembled, then the test strips of 4.0mm is cut into by automatic cutting machine, again test strips and cartridge are carried out pressure shell, then kit and drying agent, suction pipe are assembled and sealed.
Minimum detectability
The Procalcitonin quality-control product (coming from Landau company) of preparation variable concentrations, detect by this detection kit, result shows that this detection kit minimum detectability is 0.1ng/ml.About 5 times are improved than traditional collaurum method sensitivity.
Clinical examination
The clinical testing of this kit, via the routine clinical research of two provincial Grade A hospital 240, may be used for the sxemiquantitative inspection of Procalcitonin in sputum or throat swab, and the coincidence rate of blood testing is 100%.
Claims (5)
1. a carbon nano-tube Procalcitonin detection kit, is characterized in that: this kit carbon nano-tube colloidal gold composite mark Procalcitonin antibody; Test paper base plate is pasted with sample pad, carbon nano-tube colloidal gold composite pad, nitrocellulose filter and adsorptive pads successively; Described carbon nano-tube colloidal gold composite pad is attached with the Procalcitonin monoclonal antibody 1 of carbon nano-tube colloidal gold composite mark, described nitrocellulose filter is coated with detection line and the nature controlling line of Procalcitonin monoclonal antibody 2.
2. carbon nano-tube Procalcitonin detection kit according to claim 1, is characterized in that: carbon nano-tube (4) used is Single Walled Carbon Nanotube, one in multi-walled carbon nano-tubes, hydroxylation carbon nano-tube, carboxylic carbon nano-tube, water-soluble carbon nanometer tube.
3. carbon nano-tube Procalcitonin detection kit according to claim 1, is characterized in that: carbon nano-tube (4) and collaurum (3) form carbon nano-tube colloidal gold composite by self assembly.
4. carbon nano-tube Procalcitonin detection kit according to claim 1, is characterized in that: described carbon nano-tube colloidal gold composite, and wherein carbon nano-tube (4) accounts for colloidal gold composite gross mass is 0.1 ~ 20.0%.
5. the preparation method of a carbon nano-tube Procalcitonin detection kit as claimed in claim 1, it is characterized in that: the Procalcitonin monoclonal antibody 1 described carbon nano-tube colloidal gold composite pad being attached with carbon nano-tube colloidal gold composite mark, described nitrocellulose filter is coated with detection line and the nature controlling line of Procalcitonin monoclonal antibody 2; Concrete steps are as follows:
(1) preparation of carbon nano tube dispersion liquid: get the surfactant adding 0.5 ~ 5.0g in 100ml pure water, after stirring, add 10 ~ 1000mg carbon nano-tube (4), the ultrasonic 2 ~ 10min of ultrasonic cell disruptor, obtains carbon nanotube dispersed solution;
(2) preparation of carbon nano-tube colloidal gold composite: take the Procalcitonin antibody that collaurum (3) has marked, adds the above-mentioned carbon nano tube dispersion liquid of mass ratio 0.1 ~ 30.0%, stirs 5 ~ 30 minutes; Centrifugal 30 ~ 60min;
(3) preparation of carbon nano-tube colloidal gold composite pad: abandoning supernatant, then in centrifuge tube, add gold mark dilution, after mixing, by above-mentioned solution spraying on the gold mark pad processed, drying for standby;
(4) detection line, nature controlling line draw film: be affixed on base plate by NC film, stand-by; The Procalcitonin monoclonal antibody 2 of preparation 0.5mg/ml; Compound concentration is 1.0mg/ml sheep anti-mouse antibody; Respectively on ready NC film before, draw film with 1.0 μ l/cm by point film instrument again, be respectively T, C line, drying at room temperature 2 hours;
(5) assemble: more dried colloidal gold pad and thieving paper, sample pad, the base plate that posts NC film are assembled, then the test strips of 4.0mm is cut into by automatic cutting machine, again test strips and cartridge are carried out pressure shell, then kit and drying agent, suction pipe are assembled and sealed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410740721.6A CN104459117A (en) | 2014-12-08 | 2014-12-08 | Carbon nano-tube procalcitonin detection kit and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410740721.6A CN104459117A (en) | 2014-12-08 | 2014-12-08 | Carbon nano-tube procalcitonin detection kit and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104459117A true CN104459117A (en) | 2015-03-25 |
Family
ID=52905505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410740721.6A Pending CN104459117A (en) | 2014-12-08 | 2014-12-08 | Carbon nano-tube procalcitonin detection kit and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104459117A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424933A (en) * | 2016-01-26 | 2016-03-23 | 姜竹泉 | Collaurum immunochromatography test strip detecting mycoplasma pneumoniae and preparing method thereof |
| CN107543933A (en) * | 2016-06-24 | 2018-01-05 | 江苏雷森生物科技有限公司 | A kind of preparation method of carbon nano-particle of antibody labeling and the early pregnancy test strips using its preparation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202794178U (en) * | 2012-08-07 | 2013-03-13 | 天津中新科炬生物制药有限公司 | Fast quantitative immunochromatographic assay kit for procalcitonin |
| CN103278637A (en) * | 2013-06-05 | 2013-09-04 | 姜竹泉 | Carbon nanotube test paper for detecting helicobacter pylori, and preparation method thereof |
| CN104090109A (en) * | 2014-07-25 | 2014-10-08 | 胡晓武 | Colloidal gold immunochromatography test paper and colloidal gold immunochromatography test method for quickly detecting human blood procalcitonin |
-
2014
- 2014-12-08 CN CN201410740721.6A patent/CN104459117A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202794178U (en) * | 2012-08-07 | 2013-03-13 | 天津中新科炬生物制药有限公司 | Fast quantitative immunochromatographic assay kit for procalcitonin |
| CN103278637A (en) * | 2013-06-05 | 2013-09-04 | 姜竹泉 | Carbon nanotube test paper for detecting helicobacter pylori, and preparation method thereof |
| CN104090109A (en) * | 2014-07-25 | 2014-10-08 | 胡晓武 | Colloidal gold immunochromatography test paper and colloidal gold immunochromatography test method for quickly detecting human blood procalcitonin |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424933A (en) * | 2016-01-26 | 2016-03-23 | 姜竹泉 | Collaurum immunochromatography test strip detecting mycoplasma pneumoniae and preparing method thereof |
| CN107543933A (en) * | 2016-06-24 | 2018-01-05 | 江苏雷森生物科技有限公司 | A kind of preparation method of carbon nano-particle of antibody labeling and the early pregnancy test strips using its preparation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10983057B2 (en) | Method for preparing a ratiometric fluorescent sensor for paracetamol based on a copper nanoclusters-carbon dots-arginine composite | |
| CN103439497B (en) | Salmonella enrichment and rapid detection method | |
| WO2022206401A1 (en) | Detection method and detection kit for high-sensitivity sars-cov-2 neutralizing antibody | |
| CN103278637B (en) | A kind of carbon nano-tube Test paper detecting helicobacter pylori and preparation method thereof | |
| CN102103145B (en) | Colloidal gold test strip for double-amplification system and preparation method thereof | |
| CN103558271B (en) | Electrochemical biosensor for detecting penicillin and preparation method and application thereof | |
| JP4920553B2 (en) | Immunochromatographic kit | |
| CN103439495B (en) | Listeria monocytogenes enrichment and rapid detection method | |
| CN103439496B (en) | Escherichia coli O157:H7 enrichment and rapid detection method | |
| CN104582571A (en) | Quick test device and method | |
| CN101965515A (en) | Methods and device for the detection of occult blood | |
| WO2022110733A1 (en) | Fluorescent immunochromatographic test strip capable of detecting early infection of swine trichinella spiralis and preparation method therefor | |
| CN102854324B (en) | Method for rapid nondestructive detection of liver tumor marker and test paper strip adopted by the method | |
| CN106226518A (en) | Canine distemper virus colloidal gold immunochromatographydetection detection test paper bar and preparation method thereof | |
| CN103645314A (en) | Test strip card capable of automatically filtering sample | |
| CN101561436A (en) | Colloidal gold test paper for listeria monocytogenes, preparation method thereof and application thereof | |
| CN103091306B (en) | Solution for sealing and preserving magnetic nanoparticles | |
| CN105606808A (en) | Group A/B streptococcus colloidal gold immunochromatograohic assay detection device and detection method thereof | |
| CN102565163B (en) | Screen-printed electrode and multiple modification method thereof and method for detecting zearalenone | |
| CN105137066A (en) | Polystyrene latex based cancer marker immuno-chromatography kit and application method thereof | |
| CN112684169A (en) | Novel coronavirus N protein detection test strip based on immunochromatography | |
| CN204203160U (en) | The colibacillary electrochemical sensor of a kind of detection | |
| CN104459117A (en) | Carbon nano-tube procalcitonin detection kit and preparation method thereof | |
| CN206082559U (en) | A microfluid chip that outside being used for, secretes body separation, enrichment and detection | |
| CN103645321A (en) | Test paper for screening procalcitonin and preparation method of test paper |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150325 |