CN104450156A - Method for extracting grease in fructus cannabis by using aqueous enzymatic method and fructus cannabis protein drink - Google Patents

Abstract

The invention discloses an aqueous enzymatic method for extracting oil from hemp seeds. The method comprises the steps of extruding and puffing, extracting hemp protein and oil, enzymatic hydrolysis, and centrifuging to inactivate the enzyme; free oil, liquid phase and centrifugation are obtained after centrifugal separation. The hemp seed slag and the free oil are filtered and dehydrated to obtain the hemp seed oil, and the slag is used as a feed material. The present invention also provides a hemp seed protein beverage. The raw material of the beverage is the recovery and utilization of the liquid phase by-products of the oil and fat in the hemp seed extracted by the aqueous enzymatic method. obtained after sterilization. The protein content of this product is between 3% and 8%. Compared with other commercially available hemp drinks, the amount of additives in this product is greatly reduced, and it has natural plant flavor.

Description

A method for extracting oil from hemp seeds by aqueous enzymatic method and a protein drink from hemp seeds

technical field

The invention relates to the technical field of extraction processing. More specifically, it relates to a method for extracting oil from hemp seeds by aqueous enzymatic method and a protein drink from hemp seeds.

Background technique

Hemp seed is the shelled product of hemp seeds, with a fat content of 43% to 48%. In addition to oil, it also contains about 30% protein, about 5% sugar, and rich calcium, phosphorus, iron, zinc, and manganese. wait. The content of unsaturated fatty acid in hemp seed oil is more than 85%, especially the content of linolenic acid is higher, more than 16%. The ratio of linoleic acid to linolenic acid (ω-6):(ω-3) is close to 3:1, which meets the internationally recognized nutritional ratio standard; hemp seed oil can improve the activity of enzymes such as SOD/GSH-Px, and remove DPPH free The capacity of the base exceeds that of olive oil and is suitable as an antioxidant dietary resource. The main amino acids of hemp seed protein are arginine, histidine, and sulfur-containing amino acids. Arginine and histidine are very important during the growth period of children. Methionine and cysteine in sulfur-containing amino acids are the main Necessary for biological enzymes, hemp seed is one of the high-quality vegetable protein sources.

As a biological oil extraction method, aqueous enzymatic oil extraction has become a hot spot in domestic oil extraction research in recent years due to its advantages such as mild conditions, high efficiency, simple and safe operation, high oil quality, and environmental protection. In the water enzymatic extraction process, the total oil extraction rate is often used to evaluate the oil extraction rate, that is: total oil extraction rate (%) = (total oil quality of raw materials - total mass in residue) * 100% / total oil in raw materials Quality, due to the existence of the emulsion and the inability to completely break the emulsion, the general actual free clear oil yield (clear oil extraction rate (%)=free clear oil quality*100%/raw material total oil quality) is much lower than the total oil extraction rate. Therefore, reducing emulsification and increasing the yield of clear oil are very important steps in enzymatic oil extraction.

As a pre-treatment technology of vegetable oil, extrusion technology is based on the combined extrusion, kneading, shearing and other mechanical effects of the broken-toothed spiral and shearing pins in the extrusion machine, as well as frictional heat generation and steam injection. The effect of heat and humidity puts the material in an instantaneous high-temperature and high-pressure environment, thereby destroying the cell structure. When the material is extruded out of the die hole, the pressure is released instantly, the water evaporates rapidly, and the cell tissue is destroyed again. As a pretreatment technology for enzymatic oil extraction, it can work synergistically with enzymes to promote the release of oil in oil plants and facilitate extraction.

Patent CN102816631A discloses a method for extracting hemp seed oil by acid extrusion and water enzymatic method, which sprays the material with 2mol/L sulfuric acid of 2%-5% of the material weight before extrusion and expansion, and the sulfuric acid will cause damage to the equipment. certain corrosion, and at the same time, more alkali needs to be introduced into the enzymolysis system in the enzymatic hydrolysis with alkaline protease, and a high content of salt will have an adverse effect on the quality of the water phase; and the patent uses "total oil extraction rate " is used as an indicator, and the extraction rate of clear oil is not mentioned; the amount of enzyme added is based on the total amount of the mixed solution, and the amount of enzyme used is relatively large, and the reuse of the by-product liquid phase is not mentioned. Patent CN103445259A uses hemp seeds to directly grind and enzymatically hydrolyze to make a protein drink. The protein is dissolved through three steps of whole grain boiling, beating, and colloid milling, but the final amount of dissolved protein in the liquid phase is not clear. Patent CN101828739A discloses a tremella hemp seed beverage food and its preparation method. On the basis of the conventional direct use of hemp seed to make beverages, white fungus is added to play the role of thickener. During the preparation process, hemp seed The filtrate obtained by beating and filtering is prepared, and the protein content in the filtrate is not mentioned, and most of the nutrients such as fat and protein still exist in the residue, which is a great waste of overall resource utilization.

In order to make the hemp seed and other small oil industries develop healthily, we must pay attention to the overall processing efficiency of resources. Therefore, it is necessary to provide a method for extracting oil from hemp seeds by water enzymatic method that can guarantee the extraction rate of clear oil to the greatest extent. At the same time, it can also provide a method for recycling the liquid phase to make protein drinks, so as to avoid the high oil content that will lead to taste and stability. Sexually unhealthy hemp seed protein drink.

Contents of the invention

An object of the present invention is to provide a method for extracting oil from hemp seeds by an aqueous enzymatic method, which can overcome the severe emulsification, cumbersome demulsification, low yield of clear oil, and poor utilization of the liquid phase in the prior art. Sufficient problem, this method is an enzymatic oil extraction technology with high oil yield without emulsification or microemulsion.

Another object of the present invention is to provide a kind of hemp seed protein beverage, the raw material of this beverage is the recovery and utilization of the liquid phase by-product of oil and fat in hemp seed extracted by water enzymatic method, and the liquid phase is rich in protein, so this The protein content of the product is 3% to 5%. Compared with other commercially available hemp drinks, the amount of additives in this product is greatly reduced, and it has natural plant flavor.

To achieve the above object, the present invention adopts the following technical solutions:

A method for hydroenzymatically extracting oil from hemp seeds, comprising the following steps:

(1) extrusion puffing: carry out extrusion puffing after the hemp seed is coarsely crushed;

(2) Extract hemp protein and oil bodies: crush the hemp seeds after extrusion, add water to obtain a mixed solution, adjust the pH of the mixed solution to 8.0-8.5 with a dilute alkali solution, and stir and extract at 50-65°C 20～40min;

The protein in hemp seed is mainly globulin, which can be dissolved in dilute alkali solution. The dissolution of protein can promote the release of oil and improve the extraction rate of oil, but the pH should not be too high, and the excessive amount of alkali will affect the soluble matter in the system. The color and the increase of the salt concentration in the liquid phase are not conducive to the later use of the liquid phase to prepare protein beverages.

(3) Enzymolysis: After stirring and extracting, adjust the pH of the mixed solution to the optimum pH of the enzyme, add 0.5% to 2.0% of the weight of the extruded hemp seeds with alkaline protease, and perform enzymolysis for 2 to 6 hours; add the extruded 0.5% to 1.5% neutral protease by weight of the puffed hemp seeds, and enzymolysis is obtained after 2 to 4 hours;

Alkaline protease is an endoproteinase with good effect on hemp seed water enzymatic oil extraction, but it is easy to form an emulsified layer when used alone, and it is used in combination with neutral protease, almost no emulsified layer, which can greatly improve the oil and Total oil extraction rate. This is because alkaline protease and neutral protease have different action sites on proteins. When alkaline protease is used alone, there are still macromolecular proteins, which can easily be used as surfactants to promote the formation of stable emulsions. The C-terminal or N-terminal of the protein acted by protease is degraded here, reducing the molecular weight and flexibility of the protein molecule, reducing its emulsifying ability, thereby improving the extraction rate of clear oil.

Controlling the amount of alkaline protease and neutral protease and the time of enzymatic hydrolysis not only enables all kinds of enzymes to fully exert their enzymatic hydrolysis effectiveness, but also releases oil from the wrapped protein, which can save costs and reduce energy consumption.

(4) Enzyme-inactivating centrifugation: the enzymolysis solution is subjected to enzyme-inactivating treatment at 90-95°C for 5-10 minutes, and then centrifuged to obtain three parts: free oil, liquid phase and residue; the free oil can be obtained after dehydration treatment.

Enzyme-inactivating centrifugation inactivates alkaline protease and neutral protease in the system, prevents excessive protein hydrolysis, and ensures the taste of subsequent beverages. The residue can be used as feed material.

Preferably, the coarse crushing in the step (1) is crushing the hemp seed into particles with a mesh size ≥ 10 mesh.

Preferably, during the extrusion and puffing process described in step (1), the speed of the extruder is controlled to 60-90rpm, the speed of the feeder is 160-200rpm, the temperature of the casing of the puffing machine is 5-70°C, and the moisture content of the hemp seed after control is controlled to be 5.0% to 10.0%.

Due to the high oil content and protein content of hemp seeds, when the steam is large, the water content of the material will increase after absorbing water, and the sticky property will easily cause cavity blockage and affect the puffing effect. Therefore, it is particularly important to maintain an appropriate steam amount; feeding speed If the speed is too fast, the feed port will be blocked when the speed of the extruder is slow, otherwise the puffed material will be discharged intermittently. Therefore, a coordinated speed can ensure the smooth progress of the puffing; Aqueous enzymatic oil extraction has adverse effects.

Preferably, step (2) pulverizes the expanded hemp seeds to an average particle size of ≤50 μm; the weight of the added water is 3-8 times the weight of the hemp seeds;

The fineness of the crushed material affects the oil extraction rate of the aqueous enzymatic method. The smaller the particle size of the crushed material, the higher the oil extraction rate. However, if the crushing is too fine, the emulsification layer will be serious, which will increase the difficulty of demulsification.

More preferably, the weight of adding water is 4-6 times of the weight of hemp seed.

Preferably, the alkaline protease described in step (3) is selected from one or more of food grade Alcalase 2.4L, FoodPro protease, 2709 alkaline protease or papain;

The optimum pH ranges of the above-mentioned alkaline proteases are: Alcalase 2.4L6.5-8.5, FoodPro protease 9-10, 2709 alkaline protease 8.5-9.5, papain 8.0-9.5;

The neutral protease is selected from one or more of food-grade protease Alphalase, compound flavor protease or Neutrase;

The optimum pH ranges of the above-mentioned neutral proteases are: food-grade protease Alphalase 6-8, compound flavor protease 5.0-7.0, Neutrase 5.5-7.5;

Preferably, the alkaline protease is Alcalase 2.4L, and the neutral protease is Alphalase;

Preferably, the amount of the alkaline protease is 0.5%-1.5% of the weight of the hemp seed, and the amount of the neutral protease is 0.5%-1% of the weight of the hemp seed.

Preferably, the centrifugal separation in step (4) adopts a three-phase centrifuge or a horizontal centrifuge.

A hemp seed protein drink, the main raw material of the hemp seed protein drink is the liquid phase obtained in the method for extracting oil from hemp seed by aqueous enzymatic method in the present invention.

Preferably, the protein content in the hemp seed protein drink is 3%-8%.

Preferably, the raw material of the hemp seed protein beverage also includes sweetener and water, preferably, the weight of the sweetener is 1%-10% of the weight of the liquid phase; the weight of the water is 0-1 times of the liquid phase. The purpose of adding water is that the protein content in the liquid phase that comes out directly is too high. Adding water can not only properly dilute and stabilize the beverage, but also increase the output of the product to a certain extent.

The sweetener is selected from sucrose or fructooligosaccharides.

The present invention also provides a preparation method of hemp seed protein drink, which is to filter the liquid phase obtained in the method of extracting hemp seed oil by the above water enzymatic method, add water and sweetener, homogenize and sterilize Get the product hemp seed protein drink.

Preferably, the homogeneous pressure is 20-50Mpa; the sterilization condition is 121°C for 15min.

In the process of water enzymatic oil extraction, a large amount of hydrolyzed protein, soluble carbohydrates, minerals, etc. are dissolved in the liquid phase, and the prepared drink is rich in nutrition and has various effects on promoting human health. It is a good health-care functional plant nutrition drink. When blending, the sweetener fructooligosaccharide is added to make the drink taste better, and the homogenization treatment can improve the stability of the drink.

The measurement basis of each index among the present invention:

Clean oil yield (%) = clean oil quality * 100% / oil quality in raw materials

Total oil yield (%) = (mass of oil in raw material - mass of oil in residue) * 100%/mass of oil in raw material.

The beneficial effects of the present invention are as follows:

Compared with the organic solvent oil extraction process, the present invention does not use organic solvents, is environmentally friendly, and has good quality crude oil, reduces refining links, and saves equipment investment; Utilize protein to maximize the value of this resource.

Compared with other patents of hemp seed water enzymatic oil extraction, the amount of enzyme added is calculated by the percentage of raw materials, and the amount of enzyme used is small, which reduces the economic cost; using alkaline protease + neutral protease for enzymatic hydrolysis, greatly The degree of emulsification is reduced, the burden of the demulsification process is reduced, and the extraction rate of clear oil is improved; the vegetable protein drink is directly prepared by using the liquid phase, and the utilization rate of by-products is improved.

Compared with other hemp seed beverages, the invention utilizes the liquid phase rich in hydrolyzed protein after extracting the oil with the highest content in the hemp seed. Compared with other commercially available hemp drinks, the amount of additives is greatly reduced, the protein content is 3% to 8%, contains natural plant fragrance, and is suitable for drinking by different groups of people.

Description of drawings

The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings.

Fig. 1 shows the process flow chart of embodiment 1 of the present invention.

Detailed ways

In order to illustrate the present invention more clearly, the present invention will be further described below in conjunction with preferred embodiments and accompanying drawings. Similar parts in the figures are denoted by the same reference numerals. Those skilled in the art should understand that the content specifically described below is illustrative rather than restrictive, and should not limit the protection scope of the present invention.

Example 1

Weigh 2kg of shelled hemp seeds, coarsely pulverize and extrude and expand, the die aperture is 8mm, the rotating speed of the extruder is 90rpm, the rotating speed of the feeder is 200rpm, the casing temperature is 60°C, and the moisture content of the material after extrusion is 9.6%;

Take the extruded and puffed hemp seeds, coarsely pulverize them with a pulverizer, and then grind them with a mill until the particle size is ≤50 μm, weigh 1kg, add 4L of deionized water, stir evenly, adjust the pH of the system to 8.5, and the temperature is 60 ℃, extract for 35min;

Then adjust system pH to 8.5, add alkaline protease Alcalase 2.4L (addition is 2% of the hemp seed weight after extrusion puffing), after acting for 4 hours, add neutral protease Alphalase (addition is after extrusion puffing) 0.5% of the weight of the hemp seed), stirred and enzymolyzed for 3h to obtain the enzymolyzate;

Raise the temperature of the obtained enzymolysis solution to 90°C and keep it for 5 minutes to inactivate the enzyme, centrifuge at 4000rpm for 15 minutes, the enzymolysis solution is divided into three layers: clear oil, liquid phase and residue; the yield of clear oil is 86.12%, and the yield of total oil is 89.44% .

The obtained liquid phase is filtered, added with a sweetener (the addition amount is 5% of the liquid weight), an equal amount of water, homogenized at 30Mpa (2min/time, twice), filled, sterilized at 121°C for 15min, and cooled After getting drinks. The vegetable protein content in the beverage is 4.8%, and the beverage has a unique roasted aroma. Generally, the protein content in vegetable protein drinks is less than 3%, and the national industry standard is generally more than 0.6%.

Example 2

Weigh 3kg of shelled hemp seeds, carry out extrusion and puffing after coarse crushing, the die aperture is 6mm, the speed of the extruder is 70rpm, the speed of the feeder is 175rpm, the casing temperature is 55°C, and the water content of the material after extrusion is 6.2%;

Take the extruded and puffed hemp seeds, coarsely pulverize them with a grinder, and then grind them with a mill until the particle size is ≤50 μm, weigh 500 g, add 1.5 L of deionized water, stir evenly, adjust the pH of the system to 8.0, and the temperature is 60°C, extract for 30min;

Adjust the pH of the system to 8.5, add 2.4L of alkaline protease Alcalase 1% by weight of extruded and expanded hemp seeds and neutral protease Alphalase of 1% by weight of extruded and expanded hemp seeds, and slowly stir for 6 hours;

Raise the temperature of the system to 95°C and maintain it for 5 minutes to inactivate the enzyme, centrifuge at 4500 rpm for 15 minutes, and the enzymolysis liquid is divided into three layers: clear oil, liquid phase and residue; the yield of clear oil is 90.69%, and the yield of total oil is 91.06%.

The obtained liquid phase is filtered, added with a sweetener (the addition amount is 3% of the liquid phase weight), water in the same amount as the liquid phase, homogenized at 20Mpa (3min/time, 3 times), and sterilized at 121°C. get drinks. The vegetable protein content in the beverage is 6.9%, and the beverage has a unique roasted aroma.

Example 3

Take by weighing 5kg shelled hemp seeds, carry out extrusion puffing after coarse pulverization, die aperture 6mm, extruder rotating speed 80rpm, feeder rotating speed 188rpm, casing temperature is 70 ℃, and the material moisture content after extrusion puffing is 7.8%;

Take the extruded and expanded hemp seeds, coarsely pulverize them with a pulverizer, and then grind them with a mill until the particle size is ≤50 μm, weigh 1.5kg, add 7.5L deionized water, and adjust the pH of the system to 8.0 after stirring evenly. at 60°C, extract for 30 minutes;

Adjust the pH of the system to 9.0, add 1.5% of the weight of the hemp seed Alcalase 2.4L and 1% of the neutral protease Alphalase, and stir slowly for 5 hours;

The temperature of the system was raised to 95°C and kept for 5 minutes to inactivate the enzyme, centrifuged at 4500 rpm for 15 minutes, and the enzymolysis liquid was divided into three layers: clear oil, liquid phase and residue; the yield of clear oil was 89.21%, and the yield of total oil was 92.40%.

The liquid phase is filtered, added with 7% sweetener, homogenized at 35Mpa (3 min/time, 3 times), sterilized at 121°C, etc. to obtain a beverage. The vegetable protein content in the beverage is 7.6%, and the beverage has a unique roasted aroma

Example 4

Weigh 2kg of shelled hemp seeds, coarsely pulverize and extrude and expand, the die aperture is 10mm, the rotating speed of the extruder is 70rpm, the rotating speed of the feeder is 175rpm, the casing temperature is 65°C, and the moisture content of the material after extrusion is 9.6%;

Take the extruded and puffed hemp seeds, coarsely pulverize them with a pulverizer, and then grind them with a mill until the particle size is ≤50 μm, weigh 1 kg, add 5 L of deionized water, stir evenly, adjust the pH of the system to 8.5, and the temperature is 50 ℃, extract for 40min;

Regulate system pH to 8.5 then, add alkaline protease Alcalase 2.4L (add-on is 2% of hemp seed weight), after effecting 4 hours, add neutral protease Alphalase (add-on is 0.5% of hemp seed weight), Stir the enzymolysis for 3h to obtain the enzymolysis solution;

Raise the temperature of the obtained enzymolysis solution to 90°C and keep it for 5 minutes to inactivate the enzyme, and use a horizontal centrifuge to separate the enzymolysis solution into three layers: clear oil, liquid phase and residue; the yield of clear oil is 90.38%, and the yield of total oil is 93.25%.

The obtained liquid phase is filtered, added with sweetener fructooligosaccharide (addition amount is 1% of liquid phase weight), homogenized (2min/time, 2 times) at 30Mpa, filled, sterilized at 121°C for 15min, after cooling get drinks.

Example 5

Weigh 2 kg of shelled hemp seeds, coarsely pulverize and extrude and expand, the die aperture is 8mm, the speed of the extruder is 60rpm, the speed of the feeder is 160rpm, the temperature of the casing is 60°C, and the water content of the material after extrusion is 9.6%;

Take the extruded hemp seeds, pulverize them with a pulverizer, and then grind them with a mill until the average particle size is ≤50 μm; weigh 1kg, add 8L deionized water, stir evenly, adjust the pH of the system to 8.5, and the temperature is 65 ℃, extract for 20min;

Regulate system pH to 8.5 then, add alkaline protease Alcalase 2.4L (add-on is 2% of hemp seed weight), after effecting 4 hours, add neutral protease Alphalase (add-on is 0.5% of hemp seed weight), Stir the enzymolysis for 3h to obtain the enzymolysis solution;

Raise the temperature of the enzymolysis solution to 90°C and keep it for 10 minutes to inactivate the enzyme, and use a three-phase centrifuge to separate the enzymolysis solution into three layers: clear oil, liquid phase and residue; the yield of clear oil is 88.67%, and the yield of total oil is 91.73%.

The obtained liquid phase is filtered, added with sweetener sucrose (the added amount is 10% of the liquid phase weight), homogenized at 50Mpa (2min/time, 2 times), filled, sterilized at 121°C for 15min, and obtained beverage after cooling . The vegetable protein content in the beverage is 4.97%, and the beverage has a unique roasted aroma

Example 6-8

With embodiment 1, its difference is only: described alkaline protease is respectively FoodPro protease, 2709 alkaline protease or papain.

Examples 9-11

Same as Example 1, the only difference is that the neutral proteases are food-grade protease Alphalase, compound flavor protease or protease Neutrase respectively.

Example 10

The liquid phase of the hemp seed oil is extracted by an acid-extrusion-expanding water enzymatic method disclosed in CN102816631A in the prior art, the liquid phase is filtered, and the sweetener sucrose is added (the addition amount is 10% of the liquid phase weight), Homogenize under 50Mpa (2min/time, twice), fill, sterilize at 121°C for 15min, and obtain beverage after cooling. The beverage produces flocculation, cannot become a stable and uniform beverage system, and has a slightly undesirable pungent taste.

Apparently, the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those of ordinary skill in the art can also make It is impossible to exhaustively list all the implementation modes here, and any obvious changes or changes derived from the technical solutions of the present invention are still within the scope of protection of the present invention.

Claims (10)

1. the method for grease in aqueous enzymatic extraction Hemp Seed, is characterized in that, comprise the following steps:

(1) extruding puffing: carry out extruding puffing by after Hemp Seed coarse reduction;

(2) extract fiery numb albumen and grease body: pulverized by the Hemp Seed after extruding puffing, add water, obtain mixed solution, regulate pH to 8.0 ~ 8.5 of mixed solution with dilute alkaline soln, at 50 ~ 65 DEG C, stir extraction 20 ~ 40min;

(3) enzymolysis: stir after extracting and regulate pH of mixed to the suitableeest action pH of enzyme, add the Sumizyme MP of the Hemp Seed weight 0.5% ~ 2.0% after extruding puffing, enzymolysis 2 ~ 6h; Add the neutral protease of the Hemp Seed weight 0.5%-1.5% after extruding puffing, after enzymolysis 2 ~ 4h, obtain enzymolysis solution;

(4) enzyme that goes out is centrifugal: enzymolysis solution is carried out at 90 ~ 95 DEG C go out ferment treatment 5 ~ 10min, then centrifugation, obtains free oil, liquid phase and residue three part; After free oil processed and get final product.

2. the method for grease in aqueous enzymatic extraction Hemp Seed according to claim 1, is characterized in that: step (1) described coarse reduction is pulverized as order number >=10 object particle by Hemp Seed;

In step (1) described extrusion pressing puffing process, control bulking machine rotating speed 60-90rpm, feeding machine rotating speed 160-200rpm, bulking machine bushing temperature 5-70 DEG C, controlling expanded rear Hemp Seed water ratio is 5.0% ~ 10.0%.

3. the method for grease in aqueous enzymatic extraction Hemp Seed according to claim 1, is characterized in that: the Hemp Seed after expanded is crushed to median size≤50 μm by step (2); The weight adding water is 3-8 times of Hemp Seed weight;

More preferably, the weight adding water is 4-6 times of Hemp Seed weight.

4. the method for grease in aqueous enzymatic extraction Hemp Seed according to claim 1, is characterized in that: step (3) described Sumizyme MP be selected from food grade Alcalase 2.4L, FoodPro proteolytic enzyme, 2709 Sumizyme MPs or papoid one or more; Described neutral protease be selected from food-grade albumen enzyme Alphalase, compound protease or protease N eutrase one or more;

Preferably, described Sumizyme MP is Alcalase 2.4L, and neutral protease is Alphalase;

Preferably, described basic protein enzyme dosage is 0.5% ~ 1.5% of Hemp Seed weight, and neutral protein enzyme dosage is 0.5% ~ 1.0% of Hemp Seed weight.

5. the method for grease in aqueous enzymatic extraction Hemp Seed according to claim 1, is characterized in that: the described centrifugation of step (4) adopts three-phase centrifuge or horizontal centrifuge.

6. a hemp seeds protein beverage, is characterized in that: the liquid phase obtained in the main raw material of the described hemp seeds protein beverage method for grease in the aqueous enzymatic extraction Hemp Seed described in any one of claim 1-5.

7. hemp seeds protein beverage according to claim 6, is characterized in that: in described hemp seeds protein beverage, protein content is 3%-8%.

8. the hemp seeds protein beverage according to claim 6 or 7, is characterized in that: also comprise sweeting agent and water in the raw material of described hemp seeds protein beverage, and preferably, the weight of described sweeting agent is the 1-10% of described liquid phase weight; The weight of described water is 0-1 times of liquid phase.

9. the hemp seeds protein beverage according to claim 6 or 7, is characterized in that: described sweeting agent is selected from sucrose or oligofructose.

10. the preparation method of hemp seeds protein beverage as claimed in claim 8, it is characterized in that: in the aqueous enzymatic extraction Hemp Seed described in any one of claim 1-5 grease method in the liquid phase that obtains after filtering, add water, sweeting agent, obtain product hemp seeds protein beverage after homogeneous, sterilizing.