CN104434060A - Device for testing stereotyped behaviors of non-human primates - Google Patents

Abstract

The embodiment of the present invention discloses a non-human primate stereotyped behavior testing device, comprising: a behavior testing box, an animal containment box connected to the first side of the behavior testing box, and a second side connected to the behavior testing box A behavior observation room with two sides connected; wherein, the first side is adjacent to the second side. The non-human primate stereotyped behavior testing device provided by the invention can solve the problem of lack of measuring standards and testing devices for non-human primate stereotyped behavior.

Description

A device for testing stereotyped behavior of non-human primates

technical field

The invention relates to the field of medical technology, in particular to a non-human primate stereotyped behavior testing device.

Background technique

Autism is a serious neuropsychiatric disease, and its incidence is on the rise in modern society. Recent data show that the incidence rate is about 1% worldwide. Stereotyped behavior is one of the main pathological manifestations of autism. Although a large number of related studies have been done on rodent models of autism, due to the great differences in the genetic structure and physiological functions of the brains of rodents and humans, some rodent-based (such as The successful experiments in human clinical trials have all failed in human clinical trials, precisely because it is difficult to simulate the symptoms of advanced brain function impairment in mice and other rodents, which has greatly hindered the study of autism . Non-human primates have many similarities with humans in terms of genetic structure and physiological functions. Therefore, the development of autism research using non-human primates as models has been widely recognized. However, autism-related behaviors (such as stereotyped behaviors) still seriously lack quantifiable and standardized detection standards and measurement standards in non-human primates, and there is no matching behavioral testing device.

Contents of the invention

The embodiment of the present invention provides a non-human primate stereotyped behavior testing device, which can solve the problem of lack of measurement standards and testing devices for non-human primate stereotyped behavior.

The embodiment of the present invention discloses a non-human primate stereotyped behavior testing device, comprising:

a behavioral testing box, an animal holding box connected to a first side of the behavioral testing box, and a behavioral observation room connected to a second side of the behavioral testing box;

Wherein, the first side is adjacent to the second side.

In the non-human primate stereotyped behavior testing device provided in the embodiments of the present invention, the first side of the behavior testing box is connected to the animal holding box, and the side adjacent to the first side of the behavior testing box is connected to the behavior observation room It provides an experimental test device for the study of stereotyped behavior of non-human primates, and the stereotyped behavior of non-human primates can be further measured by this test device.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings that need to be used in the embodiments. Obviously, the drawings in the following description are some embodiments of the present invention. Those of ordinary skill in the art can also obtain other drawings based on these drawings without any creative effort.

Fig. 1 is a schematic structural view of a non-human primate stereotyped behavior testing device provided by an embodiment of the present invention;

Fig. 2 is the structural representation of the behavior testing box shown in Fig. 1;

Fig. 3 is a schematic structural view of the animal holding box shown in Fig. 1;

Fig. 4 is a schematic structural diagram of the behavior observation room shown in Fig. 1 .

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

The embodiment of the present invention provides a non-human primate stereotyped behavior test device, which can provide an experimental test device for the research of non-human primate stereotyped behavior, and the non-human primate can be further measured by the test device. Stereotyped behavior of animals.

Please refer to FIGS. 1 to 4. FIG. 1 is a schematic structural view of a non-human primate stereotyped behavior testing device provided in an embodiment of the present invention. FIG. 2 is a schematic structural view of the behavior test box shown in FIG. 1 , and FIG. 3 is a schematic structural diagram of the animal holding box shown in FIG. 1 , and FIG. 4 is a schematic structural diagram of the behavior observation room shown in FIG. 1 .

As shown in Figures 1 to 4, the non-human primate stereotyped behavior testing device may include:

Behavior test box 1, animal containment box 2 communicated with the first side of the behavior test box 1, and behavior observation room 3 communicated with the second side of the behavior test box 1;

Wherein, the first side is adjacent to the second side.

In the embodiment of the present invention, the behavior test box 1 is a hollow three-dimensional structure, which includes four opaque sides, a bottom surface and a box cover. The animal holding box 2 can be a hollow three-dimensional structure, or can be set to a structure of other shapes, and its sides are all made of plexiglass material, which is convenient for observers to observe the activities of the test animals in the described animal holding box 2. Animals can rest, eat and other activities in the animal holding box 2, and the animal holding box 2 should also include an entrance for allowing test animals to enter the animal holding box 2, which is not shown in the figure. The behavior observation room 3 can be a hollow three-dimensional structure, which includes four sides, a bottom surface and a top surface, and the behavior observation room 3 should also include an entrance for observers to enter the behavior observation room 3, as shown in the figure not shown.

In the embodiment of the present invention, a first through hole 010 is opened at the bottom of the first side of the behavior test box 1, and the first through hole 010 is used to communicate with the behavior test box 1 and the animal. Storage box 2 ; a second through hole 011 is opened on the second side of the behavior test box 1 , and the second through hole 011 is used to communicate with the behavior test box 1 and the behavior observation room 3 .

In the embodiment of the present invention, the non-human primate stereotyped behavior testing device may also include:

An electric control door 4 whose size is suitable for the first through hole 010, and the electric control door 4 fits the first through hole 010;

A one-way perspective glass 5 whose size is suitable for the second through hole 011, and the one-way perspective glass 5 is attached to the second through hole 011;

Wherein, the front side of the one-way see-through glass 5 is located in the behavior test box 1 , and the back side is located in the behavior observation chamber 3 .

It should be noted that the one-way see-through glass 5 is a special glass with a high reflectance to visible light, which is usually used in judicial interrogation rooms, detention centers or psychological counseling rooms. The one-way see-through glass 5 is divided into front and back sides. The front side is the reflective surface, and it must face the bright and abundant place, that is, the space that needs to be monitored. In the embodiment of the present invention, the space to be monitored is the behavior test box 1, that is, the front of the one-way perspective glass 5 is located in the behavior test box. 1; the opposite side faces the position where the monitor is located, that is, the behavior observation room 3 in the embodiment of the present invention. Wherein, the light intensity in the monitored room (i.e. behavior test box 1) should ensure sufficient brightness, the stronger the light in the behavior test box 1, the more obvious the one-way perspective effect of the one-way perspective glass 5, which is located in the behavior observation room 3 The observer can see the behavior of the test animal in the behavior test chamber 1 more clearly, and the test animal cannot see any activity in the behavior observation chamber 3, and then the behavior of the test animal will not be affected by the external environment.

In the embodiment of the present invention, several partitions 6 are fixed on the third side of the behavior test box 1, and different instruments 061-065 for non-human primate activities are placed on each of the partitions 6; Wherein, the third side is opposite to the second side.

Wherein, the second side of the behavior test box 1 is provided with a one-way perspective glass 5, and the dividing plate 6 that is placed with different apparatuses for testing animals to carry out activities is fixed on the side opposite to the second side, which can pass through the one-way perspective glass 5. The glass 5 enables the observer in the behavior observation room 3 to fully observe the behavior of the test animal, including all the behaviors of the test animal in the corner. The partitions 6 are fixed on the wall on the third side opposite to the second side of the behavior test box 1, and the walls separate these partitions 6 into separate spaces layer by layer, and the test animals can only be in one space at a time. Play on the equipment in one space, and cannot play on the equipment in two or more spaces at the same time. The left side of Fig. 2 shows different instruments placed on the partition 6, and the instruments on each partition 6 are different. Although only 5 different instruments are shown in Fig. 2, in the embodiment of the present invention, the instruments can be used according to the experiment The progress and design of the device can be increased, decreased and replaced in quantity and type, so that the number of partitions 6 also changes with the increase or decrease of the number of devices. The main feature of these instruments 061-065 is that they are fixed in a specific position and cannot be moved at will, and can be used for play and activities of test animals. In addition, the types, appearance, and functions of the placed devices should be as rich as possible to make them more diverse and personalized. The advantage of this is to discover the different preferences of different test animals for different types of activity equipment and toys.

In the embodiment of the present invention, the electric control gate 4 is connected with an electric control part 7, and the electric control part 7 is used for remotely controlling the electric control gate 4. When the non-human primate approaches the When the electric control door 4 is opened by remote control, the electric control door 4 is opened by remote control, and when the non-human primate leaves the electric control door 4, the electric control door 4 is closed by remote control.

In the embodiment of the present invention, the behavior observation room 3 may be equipped with remote control switches 081-085 for remotely controlling the different instruments 061-065 for the non-human primate to move.

Among them, the left side of FIG. 4 is the components that can be placed inside the behavior observation room 3 . Due to the diversification of functions and types of devices used for testing animals (ie, non-human primates), some devices can be simple toys that do not require external control, while some devices can be controlled through background programs Its operation can also be controlled by a fixed switch to turn it on or off. The remote switches 081-085 used to control the operation of these different instruments 061-065 can be manually controlled by the observers in the behavior observation room 3. When the test animals enter the behavior test box 1, the observers in the behavior observation room 3 will The remote control switches 081-085 controlling the different instruments 061-065 in the behavior test box 1 are all dialed to the open state; The remote control switches 081-085 of different instruments 061-065 are all set to the off state.

In the embodiment of the present invention, the behavior observation chamber 3 is provided with an electric gate control valve 086 for controlling the electric control component 7 .

Wherein, the electric control part 7 is used for opening/closing of the remote control electric control door 4, and the observer who is positioned in the behavior observation room 3 can observe the activity situation of the test animal, when the observer finds that the test animal that is positioned in the animal holding box 2 approaches the electric control door At 4 o'clock, the electric gate control valve 086 is operated immediately, and the electric gate control valve 086 sends a control command to the electric control part 7, and the electric control part 7 remotely controls the electric control gate 4, and the remote control electric control gate 4 is opened, and the test animal can enter the behavior smoothly. Test box 1; when the observer finds that the test animal enters the behavior test box 1 and is away from the electric control door 4, then operate the electric gate control valve 086 again, and the electric gate control valve 086 controls the electric control part 7 to remotely control the electric control gate 4, and the remote control Electric control door 4 is closed, and doing like this is in order to prevent test animal from returning to animal holding box 2 prematurely, and its advantage is to reduce the contact interference of observer to test animal. After passing through the initial stage of habit, the test animals, driven by their curiosity and playful nature, will actively pass through the electric control door 4 and enter the behavior test box 1 for activities, and show their behavior in a relatively relaxed state.

In the embodiment of the present invention, the behavior observation room 3 may be equipped with a camera 087 for photographing the behavior of the non-human primate and a device 088 for recording the physiological parameters of the non-human primate .

Wherein, a camera 087 can be placed in front of the one-way perspective glass 5 in the behavior observation room 3, one camera 087 can be placed, and multiple cameras 087 can be placed, so as to comprehensively photograph the test animals in the behavior test box from different angles. 1 behavioral situation. A device 088 for recording the physiological parameters of the test animal can be placed in the behavior observation room 3. The device 088 can be used to record the physiological parameters such as heart rate, brain wave, respiratory rate, blood pressure of the test animal, and can also be used to record the test animal's physiological parameters. Behavioral tracks (such as the time spent playing on different appliances), and the activity video of the test animal taken by the camera 087 can also be preserved. In addition, some equipment 089 for electrical stimulation, light stimulation, drug stimulation, etc. can also be placed in the behavior observation room 3. For example, when the test animal stays in one place for a long time, the above equipment 089 can be used. Test animals are challenged.

The working process of the non-human primate stereotyped behavior testing device provided by the embodiment of the present invention is as follows. The premise of performing the following process is that the test animal has passed the stage of adapting to the test environment in the early stage:

The test animals to be experimented with are first placed in the animal holding box 2, and after a period of adaptation, the test animals will approach the electric control gate 4, and the observer located in the behavior observation room 3 operates the electric gate control valve 086 to control the electric control unit 7 , so that the electric control part 7 remotely opens the electric control door 4, so that the test animal enters the behavior test box 1. After the test animal enters the behavior test box 1, the observer operates the electric gate control valve 086 again to control the electric control part 7, so that the test animal enters the behavior test box 1. The electric control part 7 remotely controls the electric control door 4 to close.

Observe the test animal, and record within a certain period of time (such as within 30 minutes) the time that the test animal plays on the apparatus placed on each partition 6, and the time that the test animal does not touch the apparatus in the behavior test box 1 The time spent at a certain location.

The longer the test animal stays on a certain apparatus, or the longer the time that the test animal stays in a certain position in the behavior test box 1 without touching the apparatus, the more serious the stereotyped behavior tendency of the test animal is.

After each stereotyped behavior test of the test animals, the behavior test box 1 and the animal holding box 2 must be cleaned and disinfected with alcohol, so as not to affect the test results of the next test animal stereotyped behavior by the excrement of the test animals.

The non-human primate stereotyped behavior testing device disclosed in the embodiment of the present invention has a structure in which the animal holding box and the behavior testing box are connected by an electric control door, and the behavior testing box and the behavior observation room are connected by a one-way perspective glass, It is convenient for the observer to observe the behavior activities of the test animals well, and the influence of the external environment on the behavior of the test animals is minimized. In addition, by observing and recording quantifiable indicators such as the time spent by test animals playing on several different activity equipment, heart rate, brain waves, etc., and analyzing and comparing these quantifiable indicators, it can be used for the test of stereotyped behavior. Indicators provide a measure and also extend the stereotyped behavioral monitoring paradigm in non-primates. The non-human primate stereotyped behavior test device disclosed in the embodiment of the present invention has the advantages of simple and reasonable design, convenient operation, stable performance, and low cost. Promoted and used in the school and the School of Life Sciences.

The above only specifically and in detail disclosed the preferred embodiments of the present invention, but should not be construed as limiting the patent scope of the present invention. It should be pointed out that those skilled in the art can make some modifications and improvements without departing from the concept of the present invention, and these still belong to the scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.

Claims (9)

1. A non-human primate stereotyped behavior testing device, characterized in that it comprises: a behavioral testing box, an animal holding box connected to a first side of the behavioral testing box, and a behavioral observation room connected to a second side of the behavioral testing box; Wherein, the first side is adjacent to the second side. 2. The device according to claim 1, characterized in that, The behavior test box is a hollow three-dimensional structure, and the bottom of the first side is provided with a first through hole, and the first through hole is used to communicate with the behavior test box and the animal holding box; A second through hole is opened on the second side, and the second through hole is used for connecting the behavior test box and the behavior observation room. 3. The device according to claim 2, further comprising: an electric control door whose size is suitable for the first through hole, and the electric control door fits the first through hole; a one-way perspective glass whose size is suitable for the second through hole, and the one-way perspective glass is attached to the second through hole; Wherein, the front side of the one-way see-through glass is located in the behavior test box, and the back side is located in the behavior observation room. 4. The device according to any one of claims 1 to 3, characterized in that, several partitions are fixed on the third side of the behavior test box, and each of the partitions is placed with a A different apparatus for animal activity; wherein said third side is opposite said second side. 5. The device according to claim 3, wherein the electric control gate is connected with an electric control part, and the electric control part is used to remotely control the electric control gate, when the non-human primate When approaching the electric control gate, the electric control gate is remotely opened, and when the non-human primate leaves the electric control gate, the electric control gate is remotely closed. 6 . The device according to claim 4 , wherein the behavior observation room is equipped with a remote control switch for remotely controlling the different instruments for the non-human primate to move about. 7 . 7. The device according to claim 5, characterized in that an electric gate control valve for controlling the electric control component is placed in the behavior observation room. 8. The device according to claim 1, wherein the behavior observation room is equipped with a video camera for photographing the behavior of the non-human primate and for recording the behavior of the non-human primate. physiological parameters of the device. 9. The apparatus of claim 1, wherein the animal holding box is made of plexiglass material.