CN104406965A - Kit for quickly detecting residual chlorine in water sample and use method of kit - Google Patents
Kit for quickly detecting residual chlorine in water sample and use method of kit Download PDFInfo
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- CN104406965A CN104406965A CN201410679565.7A CN201410679565A CN104406965A CN 104406965 A CN104406965 A CN 104406965A CN 201410679565 A CN201410679565 A CN 201410679565A CN 104406965 A CN104406965 A CN 104406965A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 84
- 238000000034 method Methods 0.000 title claims abstract description 20
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 title abstract description 38
- 239000000460 chlorine Substances 0.000 title abstract description 38
- 229910052801 chlorine Inorganic materials 0.000 title abstract description 38
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 83
- 238000012360 testing method Methods 0.000 claims abstract description 31
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 26
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 10
- 239000000243 solution Substances 0.000 claims description 60
- 125000001309 chloro group Chemical group Cl* 0.000 claims description 51
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical group CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 48
- 238000003756 stirring Methods 0.000 claims description 27
- HVBSAKJJOYLTQU-UHFFFAOYSA-N 4-aminobenzenesulfonic acid Chemical compound NC1=CC=C(S(O)(=O)=O)C=C1 HVBSAKJJOYLTQU-UHFFFAOYSA-N 0.000 claims description 22
- 238000012216 screening Methods 0.000 claims description 22
- WHJMGMTWMIGGQF-UHFFFAOYSA-N 1,1'-biphenyl;methanamine Chemical group NC.C1=CC=CC=C1C1=CC=CC=C1 WHJMGMTWMIGGQF-UHFFFAOYSA-N 0.000 claims description 18
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 12
- 229950000244 sulfanilic acid Drugs 0.000 claims description 11
- 239000000376 reactant Substances 0.000 claims description 10
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 9
- 239000003292 glue Substances 0.000 claims description 9
- 229940095064 tartrate Drugs 0.000 claims description 9
- -1 test tube Substances 0.000 claims description 9
- 239000006172 buffering agent Substances 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 235000005979 Citrus limon Nutrition 0.000 claims description 3
- 241000237502 Ostreidae Species 0.000 claims description 3
- 235000020636 oyster Nutrition 0.000 claims description 3
- 244000131522 Citrus pyriformis Species 0.000 claims description 2
- FEWJPZIEWOKRBE-XIXRPRMCSA-N Mesotartaric acid Chemical compound OC(=O)[C@@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-XIXRPRMCSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 238000000498 ball milling Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 7
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000000873 masking effect Effects 0.000 abstract 1
- 239000008399 tap water Substances 0.000 description 10
- 235000020679 tap water Nutrition 0.000 description 10
- 239000011259 mixed solution Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- JSYGRUBHOCKMGQ-UHFFFAOYSA-N dichloramine Chemical compound ClNCl JSYGRUBHOCKMGQ-UHFFFAOYSA-N 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- QDHHCQZDFGDHMP-UHFFFAOYSA-N Chloramine Chemical compound ClN QDHHCQZDFGDHMP-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 244000248349 Citrus limon Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000012206 bottled water Nutrition 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000022534 cell killing Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention relates to a kit for quickly detecting residual chlorine in a water sample and a use method of the kit. The kit and the method are suitable for quickly detecting the residual chlorine in the water sample. The kit for quickly detecting the residual chlorine in the water sample comprises the following components: a masking agent, a color developing agent, a color enhancing agent, a buffer agent, diluted hydrochloric acid of which the concentration is 6%, a test tube, a dropper with a rubber head, standard pH paper and a self-made color chart. Comparing a reaction liquid with the self-made color chart is only required to obtain changes of color, so that the observation is easy. Besides, the kit and the method have the advantages that the cost is low, the production is easy, the detection sensitivity is high, and the operation is simple and rapid.
Description
Technical field
The present invention relates to a kind of detection method of water sample, more specifically relating to a kind of kit for detecting chlorine residue in water sample fast and using method thereof.
Background technology
Chlorine residue refers to that water is through chlorination, after contact certain hour, and effective chlorine remaining in water.Chlorine is thrown in water, after certain hour contact, free property chlorine remaining in water and the general name of associativity chlorine.Refer to that chlorine drops into after in water, consume except a part of chlorine dose except acting on bacterium, microorganism, organism, inorganics etc. in water, also leave a part of chlorine dose, this part chlorine dose is just called chlorine residue.Chlorine residue can be divided into combined residual chlorine (refer to the compound of chlorine and ammonia in water, have NH2Cl, NHCl2 and NHCl3 tri-kinds, more stable with NHCl2, bactericidal effect is good), is combinative residual chlorine again; Free residual chlorine refers to ClO-, HClO, Cl2 etc. in water, and sterilization speed is fast, and sterilizing power is strong, but it is fast to disappear), cry again freedom chlorine residue; Total residual chlorine and combined residual chlorine and free residual chlorine sum.The effect of chlorine residue ensures Continuous sterilization, also can be polluted by anti-sealing again.Chlorine is current the most widely used sanitizer, and to carry out disinfection sterilization to tap water with chloride disinfectant, inexpensive, effective, easy to operate, well received, the whole world is general.But chlorine is good to bacterial cell killing effect, equally, other biological body cell, human body cell are also had a strong impact on.Add chlorine, as the effective sterilizing means of one, still used by the water factory in the world more than 80% at present.So, a certain amount of chlorine residue must be kept in municipal tap water, to guarantee the microbiological indicator safety of potable water.But, when chlorine and organic acid reaction, many carcinogenic accessory substances will be produced, such as methenyl choloride etc.Exceed a certain amount of chlorine, much harm will be produced to human body, and with niff, be commonly called as " chlorinated lime taste ".Expert studies discovery, has a bath ten minutes with tap water, and have four one-tenth to be suck via respiratory tract in bathroom in chlorine total amount, three one-tenth is absorbed by skin.Visible in airtight air, quite surprising via the chlorinity breathed or skin sucks.These chlorine be less readily available for absorption by the skin, the lighter can injure skin, makes that skin quality is coarse even produces itch.Heavy then the decline of the physiological function that can cause people that accumulates over a long period, the increase of cancer morbidity.Measure contents of residual chlorine and existence in water, to carrying out disinfection of drinking water work and ensureing that water hygiene safety is very important.
The mensuration of chlorine residue mainly has three kinds, portable DPD chlorine residual measurement instrument; O-tolidine colorimetric method; Online electrochemical analysis chlorine residue instrument.On-site measurement method in the exposure draft of National Standard of the People's Republic of China's " the mensuration N of water quality free chlorine and total chlorine, N-diethyl Isosorbide-5-Nitrae-phenylenediamine spectrophotometric method " and new replacement standard GB11898-89, its method is: at sample
The DPD solution of recommendation sample size 1/20 volume and the phosphate buffered solution of 1/20 volume is added in cup or pipe, then add instrument and recommend the sample of sample volume (adding the liquor kalii iodide recommending sample size 1/10 volume when surveying total chlorine again), colorimetric estimation after mixing.The detection reagent related in this assay method is all liquid reagent, according to calculating, its effective content of these liquid reagents added during mensuration in water sample is considerably less, if direct basis liquid fraction carries out content, mixing to realize reagent solids package, therefore need to change ratio of reagents, make it neither to affect water determination and can realize again good solids mixing processing technology.But the method is by the content of spectrophotometry chlorine residue, although data are accurate, be not easy to field quick detection, and need calculate by calculating, consuming time longer, not intuitively.
Summary of the invention
The object of the invention is to provide a kind of economic and practical to overcome above the deficiencies in the prior art, phenomenon change obviously, can detect chlorine residue residual quantity in water sample fast and accurately.
Technical scheme: the invention discloses a kind of kit for detecting chlorine residue in water sample fast, comprising following composition: screening agent, developer, toner, buffering agent, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
The above-described kit for detecting chlorine residue in water sample fast, screening agent is the mixed aqueous solution of tartrate and sulfanilic acid.
The above-described kit for detecting chlorine residue in water sample fast, the mass ratio of screening agent mesotartaric acid, sulfanilic acid and aqueous solution is 1:1:100 ~ 1:1:200.
The above-described kit for detecting chlorine residue in water sample fast, developer is the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.05%-0.2%.
The above-described kit for detecting chlorine residue in water sample fast, developer is add water dilution after adjacent biphenyl methylamine solid adds 30% salt acid ball milling to obtain.
The above-described kit for detecting chlorine residue in water sample fast, toner is ethyl acetate.
The above-described kit for detecting chlorine residue in water sample fast, buffering agent is the citric acid-sodium citrate solution of 0.05mol/L-0.2mol/L.
The using method of the above-described kit for detecting chlorine residue in water sample fast, comprises the steps:
Step one, gets water sample 10mL-20mL to be measured, adds 3-5 and drip screening agent in water sample, stirs to drip 3-5 after 2-3 minute and drip buffering agent;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping;
Step 3, adds 1mL-2mL developer with dropper to the solution described in step 2, stirs 5-10 minute;
Step 4, drips 2-3 to the reactant liquor described in step 3 and drips toner, stir 2-3 minute;
Step 5, observes the solution colour described in step 4, contrasts obtain respective color to determine the content of chlorine residue in water sample with self-control colorimetric card.
The using method of the above-described kit for detecting chlorine residue in water sample fast, contrasting with self-control colorimetric card described in step 5 obtains respective color to determine that the pass of color that in water sample, the content of chlorine residue is concrete and content is:
| Color | Oyster | Faint yellow | Middle yellow | Lemon yellow | Khaki | Brown color | Orange |
| Concentration (mg/L) | ＜0.05 | 0.05~0.3 | 0.3~0.5 | 0.5~1.0 | 1.0~1.5 | 1.5~2.0 | ＞2.0 |
Beneficial effect: the cost for the kit making detecting chlorine residue in water sample fast made by this method is low, and be easy to observe, make simple, phenomenon change is obvious, and intuitively measure content, detection sensitivity is high, simple and quick.
Embodiment
Embodiment 1
For detecting a kit for chlorine residue in water sample fast, comprise following composition: screening agent (mass ratio is the mixed solution of 1:1:100 tartrate, sulfanilic acid, water), 0.05% the aqueous hydrochloric acid solution of adjacent biphenyl methylamine, ethyl acetate, the citric acid-sodium citrate solution of 0.05mol/L, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
The using method of the above-described kit for detecting chlorine residue in water sample fast, comprises the steps:
Step one, gets Suzhou District 7 tap water water sample 10mL in evening, adds 3 screening agents, stir the citric acid-sodium citrate solution dripping 5 0.05mol/L after 2 minutes in water sample;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping, drips 2 watery hydrochloric acid altogether;
Step 3, adds the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.05% of 2mL to the solution described in step 2 with dropper, stir 10 minutes;
Step 4, drips 3 ethyl acetate to the reactant liquor described in step 3, stirs 3 minutes;
Step 5, observes the solution colour described in step 4, and by comparing for middle yellow with self-control colorimetric card, contents of residual chlorine is 0.3mg/L ~ 0.5mg/L.
Embodiment 2
For detecting a kit for chlorine residue in water sample fast, comprise following composition: screening agent (mass ratio is the mixed solution of 1:1:200 tartrate, sulfanilic acid, water), 0.2% the aqueous hydrochloric acid solution of adjacent biphenyl methylamine, ethyl acetate, the citric acid-sodium citrate solution of 0.2mol/L, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
The using method of the above-described kit for detecting chlorine residue in water sample fast, comprises the steps:
Step one, gets Suzhou District 12 noon tap water water sample 20mL, adds 5 screening agents in water sample, stirs the citric acid-sodium citrate solution dripping 3 0.2mol/L after 3 minutes;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping, drips 3 watery hydrochloric acid altogether;
Step 3, adds the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.2% of 1mL to the solution described in step 2 with dropper, stir 5 minutes;
Step 4, drips 2 ethyl acetate to the reactant liquor described in step 3, stirs 2 minutes;
Step 5, observes the solution colour described in step 4, and by comparing for faint yellow with self-control colorimetric card, contents of residual chlorine is 0.05mg/L ~ 0.3mg/L.
Embodiment 3
For detecting a kit for chlorine residue in water sample fast, comprise following composition: screening agent (mass ratio is the mixed solution of 1:1:150 tartrate, sulfanilic acid, water), 0.1% the aqueous hydrochloric acid solution of adjacent biphenyl methylamine, ethyl acetate, the citric acid-sodium citrate solution of 0.1mol/L, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
The using method of the above-described kit for detecting chlorine residue in water sample fast, comprises the steps:
Step one, gets Suzhou District early 9 tap water water sample 15mL, adds 4 screening agents, stir the citric acid-sodium citrate solution dripping 3 0.1mol/L after 2 minutes in water sample;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping, drips 4 watery hydrochloric acid altogether;
Step 3, adds the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.1% of 2mL to the solution described in step 2 with dropper, stir 10 minutes;
Step 4, drips 2 ethyl acetate to the reactant liquor described in step 3, stirs 3 minutes;
Step 5, observes the solution colour described in step 4, and by comparing for lemon yellow with self-control colorimetric card, contents of residual chlorine is 0.5mg/L ~ 1.0mg/L.
Embodiment 4
For detecting a kit for chlorine residue in water sample fast, comprise following composition: screening agent (mass ratio is the mixed solution of 1:1:120 tartrate, sulfanilic acid, water), 0.2% the aqueous hydrochloric acid solution of adjacent biphenyl methylamine, ethyl acetate, the citric acid-sodium citrate solution of 0.1mol/L, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
The using method of the above-described kit for detecting chlorine residue in water sample fast, comprises the steps:
Step one, gets Suzhou District early 5 tap water water sample 18mL, adds 4 screening agents, stir the citric acid-sodium citrate solution dripping 3 0.1mol/L after 3 minutes in water sample;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping, drips 2 watery hydrochloric acid altogether;
Step 3, adds the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.2% of 2mL to the solution described in step 2 with dropper, stir 8 minutes;
Step 4, drips 2 ethyl acetate to the reactant liquor described in step 3, stirs 3 minutes;
Step 5, observes the solution colour described in step 4, and by comparing for khaki with self-control colorimetric card, contents of residual chlorine is 1.0mg/L ~ 1.5mg/L.
Embodiment 5
For detecting a kit for chlorine residue in water sample fast, comprise following composition: screening agent (mass ratio is the mixed solution of 1:1:180 tartrate, sulfanilic acid, water), 0.15% the aqueous hydrochloric acid solution of adjacent biphenyl methylamine, ethyl acetate, the citric acid-sodium citrate solution of 0.08mol/L, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
The using method of the above-described kit for detecting chlorine residue in water sample fast, comprises the steps:
Step one, gets and copies the homemade water sample 13mL of tap water composition, add 3 screening agents in water sample, stirs the citric acid-sodium citrate solution dripping 4 0.08mol/L after 3 minutes;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping, drips 3 watery hydrochloric acid altogether;
Step 3, adds the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.15% of 1mL to the solution described in step 2 with dropper, stir 7 minutes;
Step 4, drips 2 ethyl acetate to the reactant liquor described in step 3, stirs 2 minutes;
Step 5, observes the solution colour described in step 4, and by comparing for oyster with self-control colorimetric card, contents of residual chlorine is < 0.05mg/L.
Embodiment 6
For detecting a kit for chlorine residue in water sample fast, comprise following composition: screening agent (mass ratio is the mixed solution of 1:1:140 tartrate, sulfanilic acid, water), 0.12% the aqueous hydrochloric acid solution of adjacent biphenyl methylamine, ethyl acetate, the citric acid-sodium citrate solution of 0.14mol/L, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
The using method of the above-described kit for detecting chlorine residue in water sample fast, comprises the steps:
Step one, gets and copies the homemade water sample 17mL of tap water composition, add 3 screening agents in water sample, stirs the citric acid-sodium citrate solution dripping 5 0.14mol/L after 3 minutes;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping, drips 4 watery hydrochloric acid altogether;
Step 3, adds the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.12% of 2mL to the solution described in step 2 with dropper, stir 10 minutes;
Step 4, drips 3 ethyl acetate to the reactant liquor described in step 3, stirs 2 minutes;
Step 5, observes the solution colour described in step 4, and by comparing for brown color with self-control colorimetric card, contents of residual chlorine is 1.5mg/L ~ 2.0mg/L.
Embodiment 7
For detecting a kit for chlorine residue in water sample fast, comprise following composition: screening agent (mass ratio is the mixed solution of 1:1:130 tartrate, sulfanilic acid, water), 0.07% the aqueous hydrochloric acid solution of adjacent biphenyl methylamine, ethyl acetate, the citric acid-sodium citrate solution of 0.18mol/L, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
The using method of the above-described kit for detecting chlorine residue in water sample fast, comprises the steps:
Step one, gets and copies the homemade water sample 11mL of tap water composition, add 4 screening agents in water sample, stirs the citric acid-sodium citrate solution dripping 5 0.18mol/L after 2 minutes;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping, drips 3 watery hydrochloric acid altogether;
Step 3, adds the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.07% of 2mL to the solution described in step 2 with dropper, stir 6 minutes;
Step 4, drips 2 ethyl acetate to the reactant liquor described in step 3, stirs 2 minutes;
Step 5, observes the solution colour described in step 4, and by comparing for orange with self-control colorimetric card, contents of residual chlorine is > 2.0mg/L.
7 kinds that detect for above embodiment 1-7 different water samples, by detecting that the concrete content of chlorine residue verifies result of the present invention, the results are shown in Table 1:
The testing result of table 1 embodiment 1-7 and concrete detected value
| Testing result of the present invention | Concrete detection numerical value | |
| Embodiment 1 | 0.3mg/L~0.5mg/L | 0.326mg/L |
| Embodiment 2 | 0.05mg/L~0.3mg/L | 0.137mg/L |
| Embodiment 3 | 0.5mg/L~1.0mg/L | 0.752mg/L |
| Embodiment 4 | 1.0mg/L~1.5mg/L | 1.233mg/L |
| Embodiment 5 | ＜0.05mg/L | 0.025mg/L |
| Embodiment 6 | 1.5mg/L~2.0mg/L | 1.658mg/L |
| Embodiment 7 | ＞2.0mg/L | 3.549mg/L |
As known from Table 1, it is entirely true that kit for detecting chlorine residue in water sample fast used in the present invention detects the numerical range obtained, and test result of the present invention only just can need be obtained by the change of reactant liquor being compared color with homemade colorimetric card, be easy to observe.Have cost low, make simple, detection sensitivity is high, simple and quick advantage simultaneously.
Claims (9)
1. for detecting a kit for chlorine residue in water sample fast, it is characterized in that, comprising following composition: screening agent, developer, toner, buffering agent, 6% concentration watery hydrochloric acid, test tube, glue head dropper, standard pH test paper and self-control colorimetric card.
2. the kit for detecting chlorine residue in water sample fast according to claim 1, is characterized in that, screening agent is the mixed aqueous solution of tartrate and sulfanilic acid.
3. the kit for detecting chlorine residue in water sample fast according to claim 1 and 2, is characterized in that, the mass ratio of screening agent mesotartaric acid, sulfanilic acid and aqueous solution is 1:1:100 ~ 1:1:200.
4. the kit for detecting chlorine residue in water sample fast according to claim 1, is characterized in that, developer is the aqueous hydrochloric acid solution of the adjacent biphenyl methylamine of 0.05%-0.2%.
5. the kit for detecting chlorine residue in water sample fast according to claim 1 or 4, is characterized in that, developer is add water dilution after adjacent biphenyl methylamine solid adds 30% salt acid ball milling to obtain.
6. the kit for detecting chlorine residue in water sample fast according to claim 1, is characterized in that, toner is ethyl acetate.
7. the kit for detecting chlorine residue in water sample fast according to claim 1, is characterized in that, buffering agent is the citric acid-sodium citrate solution of 0.05mol/L-0.2mol/L.
8. a using method for the kit for detecting chlorine residue in water sample fast described in any one of claim 1-7, comprises the steps:
Step one, gets water sample 10mL-20mL to be measured, adds 3-5 and drip screening agent in water sample, stirs to drip 3-5 after 2-3 minute and drip buffering agent;
Step 2, drips 6% watery hydrochloric acid in the solution described in step one, with stopping during standard pH test paper assaying reaction liquid pH=1 dripping;
Step 3, adds 1mL-2mL developer with dropper to the solution described in step 2, stirs 5-10 minute;
Step 4, drips 2-3 to the reactant liquor described in step 3 and drips toner, stir 2-3 minute;
Step 5, observes the solution colour described in step 4, contrasts obtain respective color to determine the content of chlorine residue in water sample with self-control colorimetric card.
9. the using method of the kit for detecting chlorine residue in water sample fast according to claim 8, it is characterized in that, contrasting with self-control colorimetric card described in step 5 obtains respective color to determine that the pass of color that in water sample, the content of chlorine residue is concrete and content is:
。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410679565.7A CN104406965A (en) | 2014-11-24 | 2014-11-24 | Kit for quickly detecting residual chlorine in water sample and use method of kit |
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| CN106546584A (en) * | 2016-11-09 | 2017-03-29 | 百奥森（江苏）食品安全科技有限公司 | The quick detection kit and using method of organochlorine residue in a kind of agricultural product |
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| CN109085162A (en) * | 2018-09-07 | 2018-12-25 | 东莞精准通检测认证股份有限公司 | A kind of chlorine residue detection method |
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| CN111504993A (en) * | 2020-05-06 | 2020-08-07 | 中国检验检疫科学研究院 | Rapid detection kit for residual chlorine after ballast water disinfection |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106546584A (en) * | 2016-11-09 | 2017-03-29 | 百奥森（江苏）食品安全科技有限公司 | The quick detection kit and using method of organochlorine residue in a kind of agricultural product |
| CN108344848A (en) * | 2018-02-06 | 2018-07-31 | 山东五洲检测有限公司 | It is a kind of to be used to detect water quality detection agent of chlorine in water and preparation method thereof |
| CN109085162A (en) * | 2018-09-07 | 2018-12-25 | 东莞精准通检测认证股份有限公司 | A kind of chlorine residue detection method |
| CN113588631A (en) * | 2018-09-07 | 2021-11-02 | 精准通检测认证(广东)有限公司 | Residual chlorine detection method |
| CN110487783A (en) * | 2019-09-08 | 2019-11-22 | 杭州陆恒生物科技有限公司 | A kind of chlorine residue detection reagent, kit and the application method of high stability |
| CN111504993A (en) * | 2020-05-06 | 2020-08-07 | 中国检验检疫科学研究院 | Rapid detection kit for residual chlorine after ballast water disinfection |
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