CN104388236B - A kind of grape wine and preparation method and application - Google Patents
A kind of grape wine and preparation method and application Download PDFInfo
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- CN104388236B CN104388236B CN201410776047.7A CN201410776047A CN104388236B CN 104388236 B CN104388236 B CN 104388236B CN 201410776047 A CN201410776047 A CN 201410776047A CN 104388236 B CN104388236 B CN 104388236B
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Abstract
Field is brewed the invention belongs to grape wine, a kind of Resveratrol content grape wine high and preparation method and application is disclosed.Grape wine of the present invention is made up of the Chinese medicine matrimony vine of integration of drinking and medicinal herbs, Poria cocos, peanut vine, sealwort, Chinese cassia tree, mulberry fruit and grape.Not only there is the health maintenance effect of grape wine, and make it have the health drug effect of Chinese medicine.The not good enough deficiency of the mouthfeel of Traditional health care wine is improved simultaneously, maintains the mouthfeel of the strong mellowness of grape wine.Experiment shows grape wine Resveratrol content of the present invention apparently higher than existing grape wine, and quality is more excellent.Grape wine of the present invention meets traditional Chinese medicine compatibility requirement, have no toxic side effect, equilibrium between yin and yang is reached with the purpose mended, it is applicable not only to male population, it is also applied for female group simultaneously, can gently kidney tonifying, liver and kidney benefiting, replenishing vital essence and blood, strengthening the bones and muscles, the effects such as having obvious establishing-Yang, improve sexuality, antifatigue, anti-aging, enhancing immune, softening blood vessel, beautifying face and moistering lotion, immune enhancing, anti-aging.
Description
Technical field
Field is brewed the invention belongs to grape wine, and in particular to a kind of grape wine and preparation method and application, especially
It is related to a kind of Resveratrol content grape wine high and preparation method and application.
Background technology
RV (Resveratrol, abbreviation Res), also known as resvertrol, chemical entitled trans -3,4,5- trihydroxy -1,
2- talan (3,4,5-trihydrolystilbence), it is the polyphenol substance for being used to resist Infected with Pathogenic Fungi in plant,
Nature is with free state RV or combines the presence of state polidatin, the content of polidatin is generally greater than its aglycon
The content of RV.RV and polidatin have dividing for cis and trans, often with the trans shape of stabilization in plant
Formula is present, and its transisomer physiologically active is better than cis-isomer, and under ultraviolet irradiation, trans RV can be converted
It is cis-isomer.The molecular structure of RV (glycosides) is as follows
RV is just found early in nineteen twenty-four, and 1940 first from Mao Yeli (Yeramtrum grandiflorum)
Root obtain, Nonomura in 1963 etc. proposes that RV is some Chinese herbal treatment inflammation, angiocardiopathy, regulation fat
The active ingredient of class metabolism etc..In recent years, increasing research shows that there is resveratrol various biological to imitate both at home and abroad
With.Resveratrol can be combined with ER in human body, adjust Blood Cholesterol level, effectively reduced people and suffered from painstaking effort
The danger of pipe disease；Resveratrol can also suppress low-density lipoprotein peroxidating and platelet aggregation, adjust lipopoteins metabolic water
It is flat, human body lipoprotein is reduced, with good prevention of arterial is atherosis and coronary heart disease effect.Resveratrol also has strong anti-oxidation
And anti-free radical function, the resveratrol of 1.3g/mL can substantially suppress the autoxidation haemolysis of Rat Erythrocytes and by H2O2Cause
Oxidative hemolysis, and have obvious inhibiting effect to mouse core, liver, brain, the inside and outside lipid peroxide of kidney.The U.S. in 1997
Scientist exists《Science》RV has been delivered on magazine, and there is the paper of active anticancer to cause the sensation of scientific circles.White black false hellebore
Alcohol has anti-mutation and antitumaous effect, can suppress the activity of cyclo-oxygenase and catalase, starting, increasing in cancer cell
Grow, develop 3 Main Stages have suppression so that reverse effect；Resveratrol can be such that the mouse skin cancer cell can at most reduces
98%, the differentiation of people's promyelocytic leukemia cell line HL-60 can be induced, it is called another new after taxol
Green cancer therapy drug.The same year, doctor Gehm etc. have found the chemical constitution of RV with a kind of female hormone-diethylstilbestrol very
It is similar, can be combined with internal ER and it is activated, play the signal transduction of female hormone, can be used as natural plant
Thing female hormone improves the osteoporosis related to female dimacteric syndrome.There is resveratrol obvious anti-radiation protection to make
With, and influence skeletal metabolism etc..Additionally, resveratrol and its derivative in liver protecting, activate longevity gene, prevent and treat nerve
The aspects such as disease also play an important role.Therefore RV has turned into world today's functional food, medicine and other fields research
One of focus.
Langcake in 1976 and Pryce have found there is RV in grape leave, and its synthesis is being subjected to ultraviolet photograph
Penetrate, mechanical wounding and drastically strengthen during fungal infection, can resist grey mold the grape pathogen such as downy mildew infect, be referred to as plant
Thus antibiotic, RV starts to be paid attention to by grape breeding scholar and phytopathologist.World health group in 1989
" the world angiocardiopathy control system " research for knitting that (WHO) carried out draws " French antinomy ", and French habits and customs are included
Trigger the high risk factor-high cholesterol of coronary heart disease, smoking and lack motion, but their coronary heart disease the incidence of disease than North America people
It is much lower, research find this with Frenchman love to drink red wine relevant because being rich in the white multitude of physiologically active ingredient in red wine
Reed alcohol.The resveratrol in grape wine has important health-care efficacy to human body as can be seen here.
The sales volume of current grape wine is growing, and product category is various, and the quality good and the bad mixes.Current domestic common extra dry red wine Portugal
The content of the resveratrol in grape wine is in 0.3-2.67mg/L, and the preferable health care dosage of resveratrol is every daily ingestion 2-
10mg/ days, to reach health-care effect needed to drink substantial amounts of claret, but substantial amounts of grape wine of drinking can cause to take in
Many alcohol, and do not meet noble, the elegant culture of sampling wine of grape wine.
The preparation of wine of resveratrol amount has been had been improved at present, can be mainly summarized as following several
Class：
1st, grape wine is prepared by raw material of the grape of Resveratrol content high.Such as Application No. CN200610129321.7
Chinese patent, the invention induces resveratrol by choosing the grape variety rich in resveratrol and ultraviolet radioactive in specific producing region
Synthesis, using the resveratrol in specific technique as much as possible dissolution Grape Skin, and farthest prevent white black false hellebore
The oxidation of alcohol, vinosity exquisiteness alcohol is with elegant fragrance is noble, in limpid ruby red.The method of the invention and common grape
Wine brewage technology does not conflict, and is not required to specific equipment, and technical arrangement plan quite facilitates, and social benefit is huge.White black false hellebore high
The grape variety of alcohol is ultraviolet radioactive induction, and its toxigenic capacity is high, and the proterties of resveratrol high is not easy hereditary succession, one
After the proterties of denier resveratrol high disappears, it is impossible to the grape wine of continuous production resveratrol high.
2nd, to adding resveratrol in grape wine.The side as disclosed in the Chinese patent of Application No. CN200880122683.0
Method includes：The resveratrol of grape is obtained to addition in a part for bulk wine, resveratrol concentrate is formed, and to
The resveratrol concentrate is added in bulk wine, the increased grape wine of resveratrol is produced.The production cost of the method
It is low.As disclosed production of high content white veratrum alkaloids spirit tool in the Chinese patent specification of Application No. CN03112486.0
Body implementation method discloses one ton of high-content of resveratrol drinks production instance：Increase by every liter of grape wine Resveratrol content
10mg is calculated, and need to prepare 10 grams of resveratrol, and grape wine 1000kg takes grape wine in right amount, and low temperature distillation obtains more than 70%v/v Portugals
Grape alcohol liquid 50mL, by the 10g resveratrols above-mentioned grape alcohol liquid of addition, after after fully dissolving, adds 1000mL grape wine
In, it is full and uniform after, during this wine then added into one ton of grape wine, be uniformly mixed, high-content of resveratrol grape is obtained
Wine.The above method needs to be additionally provided resveratrol, and resveratrol if it is natural extract if relatively costly, and natural money
Source is limited, and the method for chemical synthesis provides resveratrol meeting environment and adversely affects, and does not meet the theory of environmental protection.
3rd, polydatin is changed into resveratrol.The Chinese patent of Application No. CN 201210539571.3 is opened
A kind of method that resveratrol is enriched with during liquor brewing, methods described includes：By brewed wine, in Aspergillus and/or
Progressively fermentation generates alcohol and various accessory substances under saccharomycete effect, while the polydatin of addition changes into resveratrol.
The method will not change the fermentation technique of wine, but it is glucuroide that polydatin changes into the enzyme of resveratrol, so nothing
Method ensures that polydatin changes into the conversion ratio of resveratrol.
4th, the Resveratrol content in grape is improved.Announced in the Chinese patent of Application No. CN 201010509046.8
It is a kind of raise grape leaf, grape stem and in grape fruit Resveratrol content method, with colloidal aluminum derivant to grape
Material is induced.Resveratrol is raised during inducible grape fruit and grape produce related by-products (blade, stem), efficiently, surely
Fixed, low-residual, low toxicity.But harm of the residual of aluminium salt to human body can not be ignored, and a large amount of pollutions of the aluminium salt to environment
It is also quite serious.
Therefore, content of the resveratrol in grape wine into a new research direction how is improved.
The content of the invention
In view of this, the present invention for prior art defect, there is provided a kind of Resveratrol content grape wine high and its
Preparation method and purposes.
To realize the purpose of the present invention, the present invention is adopted the following technical scheme that：
A kind of grape wine, is made up of following raw material：50 parts -100 parts of matrimony vine, 40 parts -100 parts of Poria cocos, peanut vine
50 parts -100 parts, 50 parts -70 parts of sealwort, 25 parts -50 parts of Chinese cassia tree, 45 parts -60 parts of mulberry fruit, 20000 parts -34000 parts of grape.
It is present invention also offers a kind of preparation of wine, the weight portion of 20000 weight portion -34000 is preprocessed
Grape and the weight portion matrimony vine of 50 weight portion -100, the weight portion Poria cocos of 40 weight portion -100, the weight portion peanut of 50 weight portion -100
Rattan, the weight portion sealwort of 50 weight portion -70, the weight portion Chinese cassia tree of 25 weight portion -50, the mixing of the weight portion mulberry fruit of 45 weight portion -60 are carried out
Alcoholic fermentation, after through after fermentation, ageing, after allocate；
Or the pretreated grape of weight portion of 20000 weight portion -34000 first carried out into alcoholic fermentation, after sent out after
Ferment, ageing, then with the weight portion matrimony vine of 50 weight portion -100, the weight portion Poria cocos of 40 weight portion -100, the weight of 50 weight portion -100
Part peanut vine, the weight portion sealwort of 50 weight portion -70, the weight portion Chinese cassia tree of 25 weight portion -50, the weight portion mulberry fruit of 45 weight portion -60 mix
Close allotment.
In some embodiments, grape described in the preparation method is Merlot, Cabernet Sauvignon.
In some embodiments, pretreatment described in the preparation method is to be sprayed after treatment with ultraviolet using salicylic acid
The synthesis of resveratrol in line radiation-induced grape.
In some embodiments, in the preparation method, the SA concentration is 60mg/L-100mg/L, the UV inductions
Amount 0.9KJ/m2-1.28KJ/m2, -430 seconds 310 seconds.
In some embodiments, in the preparation method, the alcoholic fermentation is 18%- for adjustment grape juice sugar content
25%, acid content is 0.6%-0.8%, be inoculated with the yeast of 0.3wt%-0.5wt%, 0.3wt%-0.5wt% pectases and
0.3wt%-0.5wt% glucuroides are fermented, and control fermentation temperature below 28-30 DEG C during fermentation, and fermentation is extremely sent out
Zymotic fluid residual sugar is down to less than 0.06%.
In some embodiments, in the preparation method, the yeast is saccharomyces bayanus.
In some embodiments, in the preparation method, the pectase is HE GRAND CRU pectases.
Present invention also offers grape wine obtained in the preparation method.
Present invention also offers purposes of the grape wine in the medicine for preparing treatment kidney deficiency.
Grape wine of the present invention is by the Chinese medicine matrimony vine of integration of drinking and medicinal herbs, Poria cocos, peanut vine, sealwort, Chinese cassia tree, mulberry fruit and grape
It is made.Not only there is grape wine health maintenance effect in itself, and make it have the health drug effect of Chinese medicine.Also improve biography simultaneously
Unite the not good enough deficiency of mouthfeel of health liquor, maintain the mouthfeel of the strong mellowness of grape wine.Experiment shows grape of the present invention
Wine Resveratrol content is apparently higher than import and domestic grape wine, and quality is more excellent.Grape wine of the present invention meets in the traditional Chinese medical science
The requirement of medicine compatibility, has no toxic side effect, and reaches equilibrium between yin and yang with the purpose mended, and male population is applicable not only to, while being also applied for
Female group, can gently kidney tonifying, liver and kidney benefiting, replenishing vital essence and blood, strengthening the bones and muscles, have obvious establishing-Yang, improve sexuality, antifatigue, anti-aging,
The effects such as strengthening immune, softening blood vessel, beautifying face and moistering lotion, immune enhancing, anti-aging, significant curative effect is played to deficiency of the kidney, is fitted
It is suitable for that tired body, soreness of waist and knee joint, chilly are cold, impotence is not lifted, the kidney deficiency crowd of insomnia and dreamful sleep.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
To realize the purpose of the present invention, the present invention is adopted the following technical scheme that：
A kind of grape wine, is made up of following raw material：50 parts -100 parts of matrimony vine, 40 parts -100 parts of Poria cocos, peanut vine
50 parts -100 parts, 50 parts -70 parts of sealwort, 25 parts -50 parts of Chinese cassia tree, 45 parts -60 parts of mulberry fruit, 20000 parts -34000 parts of grape.
Wherein, the matrimony vine refers to commodity " fruit of Chinese wolfberry ", substantially refers to the dry mature fruit from lycium barbarum.
Matrimony vine is mild-natured, sweet, returns liver and kidney channel, has functions that nourishing liver and kidney, benefiting shrewd head, for consumption consumptive loss, soreness of waist and knee joint, dizzy
The diseases such as dizzy tinnitus, impotence and seminal emission, Heat Diabetes, blood deficiency chlorosis, blurred vision.
Poria cocos, is the dry sclerotia of On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf.Poria cocos is sweet, light,
It is mild-natured, the thoughts of returning home, lung, spleen, kidney channel, the effect of with clearing damp and promoting diuresis, invigorating the spleen, calming heart, for oedema oliguria, phlegm retention anti-dazzle nervous, insufficiency of the spleen
The diseases such as deficiency of food, loose stool diarrhea, confused and worried, insomnia of palpitating with fear.
Sealwort (scientific name：Polygonatum sibiricum), also known as：Polygonatum sibiricum Redoute, yellow chicken dish, shaft of a writing brush dish, claw ginseng,
Tendrilleaf solomonseal rhizome, achickenclaw ginseng.It is the rhizome of Liliaceae Polygonatum sealwort, P. cyrtonema, hot river sealwort, P. kingianum, polygonatum cirrhifolium Royle etc..It is yellow
Smart nature and flavor are sweet, with tonifying spleen, the effect of moistening lung is promoted the production of body fluid.
Chinese cassia tree (Classification system：Cinnamomum cassia Presl), also known as beautiful osmanthus, male osmanthus, cajaput, great Gui, peppery osmanthus,
Safety tree, cassia, are the dry bark of canella Chinese cassia tree.Complement sun, warming spleen and stomach, removing internal cold, promoting blood circulation.Control gate of vitality's fire
Decline, cold weak pulse of limb, yang depletion collapse, diarrhea of suffering from abdominal pain, cold hernia run quickly globefish, waist and knee crymodynia, Amenorrhea abdominal mass, cloudy subcutaneous ulcer, streamer, and empty sun is floating
More, heat in the upper and cold in the lower.
Mulberry fruit is the ripening fruits of Moraceae deciduous tree mulberry tree, and what kidney deficiency and liver and deficiency of blood essence were lost has a dizzy spell；Soreness of waist ear
Ring；Poliosis；Insomnia and dreamful sleep；Injury thirst；Quench one's thirst；Dry constipation of intestines.
Grape wine of the present invention is by the Chinese medicine matrimony vine of integration of drinking and medicinal herbs, Poria cocos, peanut vine, sealwort, Chinese cassia tree, mulberry fruit and grape
It is made.Not only there is grape wine health maintenance effect in itself, and make it have the health drug effect of Chinese medicine.Meanwhile, also improve
The deficiency that the mouthfeel of Traditional health care wine is not good enough, maintains the mouthfeel of the strong mellowness of grape wine.Experiment shows Portugal of the present invention
Grape wine Resveratrol content is apparently higher than import and domestic grape wine, and quality is more excellent.
It is present invention also offers a kind of preparation of wine, the weight portion of 20000 weight portion -34000 is preprocessed
Grape and the weight portion matrimony vine of 50 weight portion -100, the weight portion Poria cocos of 40 weight portion -100, the weight portion peanut of 50 weight portion -100
Rattan, the weight portion sealwort of 50 weight portion -70, the weight portion Chinese cassia tree of 25 weight portion -50, the mixing of the weight portion mulberry fruit of 45 weight portion -60 are carried out
Alcoholic fermentation, after after after fermentation, ageing allocate；
Or the pretreated grape of weight portion of 20000 weight portion -34000 first carried out into alcoholic fermentation, after sent out after
Ferment, ageing, then with the weight portion matrimony vine of 50 weight portion -100, the weight portion Poria cocos of 40 weight portion -100, the weight of 50 weight portion -100
Part peanut vine, the weight portion sealwort of 50 weight portion -70, the weight portion Chinese cassia tree of 25 weight portion -50, the weight portion mulberry fruit of 45 weight portion -60 mix
Close allotment.
Medicine fermentation process together with fresh grape can not only retain the nutritional ingredient in raw material, can also obtain many micro-
The benefit materials that biological metabolism is produced, it is highly beneficial to health.
Experiment shows, the medicinal material is fermented into obtained grape wine Resveratrol content together with fresh grape apparently higher than fresh
With Chinese medicine mixed preparing grape wine after grape fermentation.Therefore, in some preferred embodiments, the preparation of wine
Be by the pretreated grape of the weight portion of 20000 weight portion -34000 and the weight portion matrimony vine of 50 weight portion -100,40 weight portions -
100 weight portion Poria cocos, the weight portion peanut vine of 50 weight portion -100, the weight portion sealwort of 50 weight portion -70, the weight of 25 weight portion -50
Part Chinese cassia tree, the weight portion mulberry fruit of 45 weight portion -60 are mixed into row alcoholic fermentation, after allocated after after fermentation, ageing.
Wherein, in some embodiments, the medicinal material mixed with grape pulverizes and sieves in advance.
In some preferred embodiments, the sieving was 14 mesh sieves.
In preparation method of the present invention, the grape can select any grape product well known by persons skilled in the art
Kind.In some embodiments, grape described in preparation method of the present invention is Merlot or Cabernet Sauvignon.
In some embodiments, pretreatment described in the preparation method is to be sprayed after treatment with ultraviolet using salicylic acid
The synthesis of resveratrol in line radiation-induced grape.
In some embodiments, in the preparation method, the amount of application of the SA sprays 100mlSA for per kilogram grape
Solution,
In some embodiments, in the preparation method, the SA concentration is 60mg/L-100mg/L.
In some embodiments, in the preparation method, the UV inducing amount is 0.9KJ/m2-1.28KJ/m2、310
- 430 seconds seconds.
In certain embodiments, in the preparation method, the grape is Merlot, and SA concentration is 100mg/L, UV
Inducing amount is 430 seconds, 1.28KJ/m2。
In certain embodiments, in the preparation method, the grape is Cabernet Sauvignon, and SA concentration is 60mg/L, UV
Inducing amount is 310 seconds, 0.9KJ/m2。
Grape deposits 72h in darkroom after treatment after being irradiated through ultraviolet, impurity elimination after then washing, broken that grape slurry is entered
Row alcoholic fermentation.
Wherein, in some embodiments, the washing is first to be cleaned with light water, then is cleaned with deionized water three times.
In some embodiments, the impurity elimination is specially using craft or destemming disintegrating machine removal carpopodium.
In some embodiments, in the preparation method, the alcoholic fermentation is 18%- for adjustment grape juice sugar content
25%, acid content is 0.6%-0.8%, be inoculated with the yeast of 0.3wt%-0.5wt%, 0.3wt%-0.5wt% pectases and
0.3wt%-0.5wt% glucuroides are fermented, and control fermentation temperature below 28-30 DEG C during fermentation, and fermentation is extremely sent out
Zymotic fluid residual sugar is down to less than 0.06%.
In some preferred embodiments, the yeast fermenting power (CO2) ＞ 950ml, moisture content ＜ 5%.
In some preferred embodiments, the pectase enzyme activity 400,000.
In some preferred embodiments, the glucuroide enzyme activity 100,000.
Daily stirring 2-4 times in wherein described alcoholic fermentation is so that in the medicinal powder and skin slag press-in grape juice for floating.
In some embodiments, the method for the adjustment grape juice sugar content and acid content is in broken grape slurry
Sulfurous acid solution 0.066%-0.117% is added, makes sulfur dioxide concentration up to 0.004%-0.006%, add sucrose
11.9wt%-23.4wt%, so that the alcoholic strength after fermentation is controlled at 7 ° -18 °.
In some preferred embodiments, in the preparation method, the yeast is saccharomyces bayanus.
In some preferred embodiments, in the preparation method, the pectase is HE GRANDCRU pectases.
In preparation method of the present invention, after fermentation and ageing are carried out after alcoholic fermentation.
In some embodiments, the after fermentation is that, in room temperature, filtering wine liquid separates skin slag by broth temperature control
Original wine is obtained, the sulfur dioxide that 0.3%-0.5% is added in original wine carries out after fermentation.
Wine liquid is filled into liquor storage vessel sealing preserve after after fermentation, temperature control is in 18-25 DEG C, period people in the art
Member can as needed carry out malic-lactic acid fermentation.
In some embodiments, the ageing is that control temperature carries out ageing, relative humidity 85%- at 10-15 DEG C
90%, and carry out down bucket, bucketful and clarifying treatment.
Preparation method of the present invention, allocates after ageing to obtained wine liquid.
In some embodiments, pol, wine degree, the acidity for being allocated as determining original wine, according to the physics and chemistry of finished product
Index is adjusted to setting respectively to be carried out blending treatment, is stored 20-30 days.
Preparation method of the present invention, also includes the steps such as refined filtration, sterilizing packing after allotment.
In some embodiments, the refined filtration is that wine liquid is crossed into 0.45 μm of filter membrane to carry out refined filtration.
In some embodiments, the sterilizing is packed as being passed through sterilizer 90 DEG C/min quick sterilizations, and bottling is sealed,
Sterilization 20min in 70~72 DEG C of water.
Alcoholic fermentation described in preparation method of the present invention and after fermentation can be at well known by persons skilled in the art
Anticipate and carry out in a kind of fermentation tank, such as good Buddhist nun's virtue fermentation tank, vertical spray fermentation tank.
In some embodiments, the fermentation tank is preferred Ni Meide fermentation tanks.
Present invention also offers grape wine obtained in the preparation method.
The mouse test result of grape wine of the present invention shows that security maintains an equal level with the grape wine for being not added with medicinal liquor fermentation,
It can significantly improve mouse sperm quantity, activity and survival rate, increase mouse testis and epididymis tissue weight, correct sun
Empty Mouse Weight, plasma cortisol, adrenal gland weight, improve mouse resist oxygen lack and Burden-Swimming Ability of KM etc., while these results
The medicinal liquor being made better than existing health care liquor for strengthening Yang (i.e. kidney-replenishing healthy medicated wine) and using existing establishing-Yang formula of Chinese materia medica preparation,
Show grape wine energy yin yang complementarity of the present invention, equilibrium between yin and yang in mediation body can nourishing blood and tranquilization, reinforcing the kidney and controlling noctural emission, to kidney deficiency
The all diseases and sub-health state for causing have obvious curative effects.
Additionally, of the present invention have level conditions to take obvious curative effects through clinical Patients with Kidney Deficiency Type：
A, taken through clinical 90 impotence patients, the patient of 30-45 days 97% feels to refresh oneself, energetic, continuously takes 6
Individual month, 95% patient can carry out normal sexual life, stop drink and do not rebound；
B, taken through clinical 10 patients, continuously taken 3 months, 8 patient's sperm quantities increase 30-55%；Through clinic
25 cold sperm patients take, and the patient of 20-30 days 97% feels that cold sperm phenomenon is alleviated, and in 2-3 month, 96% patient is without cold sperm
Phenomenon occurs；
C, taken through clinical 12 rheumatism, rheumatoid, arthritics, even drink the 30-45 days disease sites of 96% patient
Pain substantially mitigates, and even 4 months 94% patients of drink represent that disease sites are without obvious pain during drinking；
D, the patients that frequently got up in the night to urinate through clinical 23 take, and take 30-45 days 99% patients frequency of getting up in the night to urinate and significantly reduce, even drink
98% patient is without symptom of getting up in the night to urinate after 3 months, and stops not rebounded after drinking；
E, taken through clinical 9 dysmenorrhoea patients, even 2-3 month 97% patient of drink represents that dysmenorrhoea substantially mitigates, even drink 3 months
Afterwards, also without obvious dysmenorrhoea sense after 96% patient stops drinking；
F, taken through clinical 12 patients, even 3-5 month 95% patient of drink represents that the elasticity and glossiness of skin have been carried
Height, continuously drinks, and 94% patient's sensory effect is obvious：
Therefore the purposes present invention also offers grape wine of the present invention in the medicine for preparing treatment kidney deficiency.
Those skilled in the art can be according to the age of user, body weight, sex, natural health situation, nutrition condition
Deng the decision specific instructions of taking of grape wine of the present invention.In some embodiments, the grape wine side of taking of the present invention
Method is daily 50-100ml, takes optimal when having a meal, and even once week visible obvious curative effects, long-term taking can promote longevity drink, nothing
Side effect.
Grape wine of the present invention is by the Chinese medicine matrimony vine of integration of drinking and medicinal herbs, Poria cocos, peanut vine, sealwort, Chinese cassia tree, mulberry fruit and grape
It is made.Not only there is grape wine health maintenance effect in itself, and make it have the health drug effect of Chinese medicine.Also improve biography simultaneously
Unite the not good enough deficiency of mouthfeel of health liquor, maintain the mouthfeel of the strong mellowness of grape wine.Experiment shows grape of the present invention
Wine Resveratrol content is apparently higher than import and domestic grape wine, and quality is more excellent.Grape wine of the present invention meets in the traditional Chinese medical science
The requirement of medicine compatibility, has no toxic side effect, and reaches equilibrium between yin and yang with the purpose mended, and male population is applicable not only to, while being also applied for
Female group, can gently kidney tonifying, liver and kidney benefiting, replenishing vital essence and blood, strengthening the bones and muscles, have obvious establishing-Yang, improve sexuality, antifatigue, anti-aging,
The effects such as strengthening immune, softening blood vessel, beautifying face and moistering lotion, immune enhancing, anti-aging, significant curative effect is played to deficiency of the kidney, is fitted
It is suitable for that tired body, soreness of waist and knee joint, chilly are cold, impotence is not lifted, the kidney deficiency crowd of insomnia and dreamful sleep.In addition by the tune to kidney
Saving various diseases such as not smooth to the protrasion of the lumbar intervertebral disci, blockage of main and collateral channels, rheumatism chill, physically weak chilly, irregular menstruation, qi and blood has slow
Solution and improvement.The grape wine formula that the present invention is provided is without any non-traditional Chinese medicine ingredients, while these Chinese medicine excellent in taste, itself
Taste with sweetness, and do not influence grape wine mouthfeel in itself, and medicine active ingredient effect in itself and grape wine are in itself
Effect.
For a further understanding of the present invention, the grape wine provided the present invention with reference to embodiment and preparation method thereof enters
Row is described in detail.Wherein HE GRAND CRU pectases are purchased from laffort companies of France；Saccharomyces bayanus is purchased from all preferably biological section in Shanghai
Skill Co., Ltd.
Embodiment 1：Preparation method of the present invention prepares grape wine
1st, each 50 grams of matrimony vine, Poria cocos, peanut vine, sealwort, Chinese cassia tree, mulberry fruit Chinese medicine is weighed respectively, crushes the sieve of 14 mesh,
It is fitted into good Buddhist nun's virtue fermentation tank.
2nd, 20Kg fresh grapes (Merlot, Cabernet Sauvignon etc.) are taken in addition, are first sprayed after treatment with ultraviolet using salicylic acid (SA)
The synthesis of resveratrol in line (UV) radiation-induced grape.Specific method is：
1), 20Kg fresh grapes spray treatment using salicylic acid (SA), and per kilogram grape sprays 100mlSA solution, and SA is sprayed
Suitable concentration Merlot for 100mg/L, Cabernet Sauvignon be 60mg/L process after darkroom deposit 72h；
2) grape after SA sprays treatment, is taken out, with the synthesis in ultraviolet (UV) radiation-induced grape from veratryl alcohol, UV
Induction optimal dose Merlot is 430 seconds (1.28KJ/m2), Cabernet Sauvignon be 310 seconds (0.9KJ/m2), treatment is after darkroom storage
72h；
3) grape of UV irradiations, is taken out, is first cleaned with light water, then cleaned with deionized water three times, using craft or removed
Stalk disintegrating machine removal carpopodium, fruit is crushed and obtains grape slurry, in the good Buddhist nun's virtue fermentation tank of addition.
3rd, sulfurous acid 0.066% is added in broken grape slurry, makes sulfur dioxide concentration up to 0.004%, add 3.6kg
Sucrose, stirs, and adjustment grape juice sugar content is 18%, and acid content is 0.6%, is inoculated with the saccharomyces bayanus (fermenting power of 0.3wt%
(CO2) ＞ 950ml moisture content ＜ 5%) and 0.3wt%HE GRAND CRU pectases (enzyme activity 400,000) and 0.3wt% glucose
Glycosides enzyme (enzyme activity 100,000) is fermented, and controls fermentation temperature below 28-30 DEG C during fermentation, and fermentation to zymotic fluid residual sugar is dropped
Fermented to less than 0.06%, control fermentation temperature below 28-30 DEG C during fermentation, stirring 2-4 times, is made floating daily
In medicinal powder and skin slag press-in grape juice；
4th, 9 angel's zymotic fluid residual sugars of fermentation are down to less than 0.06%, and broth temperature control is separated in room temperature, filtering wine liquid
Skin slag obtains original wine；The sulfur dioxide that 0.3% is added in original wine carries out after fermentation；
5th, wine fills liquor storage vessel sealing preserve, and at 18-25 DEG C, period carries out malic-lactic acid fermentation to temperature control as needed；
6 then control temperature carry out ageing, relative humidity 85%-90% at 10-15 DEG C, and carry out down bucket, bucketful and clear
Clear treatment；
7th, pol, wine degree, the acidity of original wine are determined, setting is adjusted to respectively according to the physical and chemical index of finished product and is hooked
Treatment is converted, is stored 28 days；Wine liquid is crossed into 0.45 μm of filter membrane carries out refined filtration, is passed through sterilizer 90 DEG C/min quick sterilizations, bottles close
Envelope, the sterilization 20min in 70-72 DEG C of water, inspection is qualified can code, vanning, storage.
Embodiment 2：Resveratrol content is determined
Sample：Trans-resveratrol standard items (purity is more than 99%), purchased from Sigma-Aldrich companies.
Reagent：Methyl alcohol, acetonitrile are chromatographically pure, and remaining reagent is pure for analysis.
Instrument and equipment：Waters e2695 liquid chromatographs (equipped with Waters2998 PDADs and
Empower data handling systems, Waters, US)；Chromatographic condition：Chromatographic column is Ultimate C18Post (4.6*250mm,
5 μm) mobile phase As be acetonitrile, Mobile phase B be 0.01% phosphate aqueous solution, isocratic elution, A:B=25:75(v/v).Flow velocity
1.0m L/min, sample size is 20 μ L, and Detection wavelength is 306nm, and column temperature is 35 DEG C.
Sample treatment：Example 1 is obtained wine samples and commercial glucose wine sample respectively, and centrifugal concentrating removes second
Alcohol, plus 10mL deionized waters, enzymolysis liquid is extracted with 10mL ethyl acetate, is extracted 4 times, is collected extract centrifugal concentrating and is removed acetic acid
Ethyl ester, with 250 μ L mobile phase (acetonitriles：0.01% phosphate aqueous solution=25:75 (v/v)) dissolving after as test liquid.
The preparation of standard solution：25mg trans-resveratrol standard items are weighed, 25m L are dissolved and be settled to methyl alcohol,
It is made 1L storing solutions.Take appropriate storing solution methanol dilution into concentration be 0.2mg/L, 0.5mg/L, 1.0mg/L, 1.5mg/L,
The trans-resveratrol standard solution of 2.0mg/L, 5.0mg/L, 10mg/L.
Irradiation 2h at 365nm uviol lamps is placed on, trans-resveratrol is partially converted into cis-resveratrol, and lead to
Cross differential technique and calculate cis-resveratrol and trans-resveratrol content.
The content of the resveratrol in wines of table 1
The quality of grape wine is evaluated generally using Resveratrol content as important reference index, can from the result of table 1
Go out, commercially available import grape wine is higher than domestic grape wine Resveratrol content, and quality is good, and domestic grape wine is due to manufacture craft and guarantor
The deficiency in method is deposited, causes resveratrol to lose more serious.And the grape wine Resveratrol content of present invention offer is substantially high
In import and domestic grape wine, quality is more excellent.
Embodiment 3：
20Kg fresh grapes are taken by the methods described of embodiment 1, ultraviolet (UV) is used after first spraying treatment using salicylic acid (SA)
The synthesis fermentation of resveratrol prepares grape wine in radiation-induced grape；Then matrimony vine, Poria cocos, peanut vine, sealwort, meat are weighed
Each 50 grams of osmanthus, mulberry fruit Chinese medicine, crushed the sieve of 14 mesh, and the grape wine prepared with fermentation mixes, and determined pol, the wine of original wine
Degree, acidity, being adjusted to setting respectively according to the physical and chemical index of finished product carries out blending treatment, stores 28 days；Wine liquid is crossed into 0.45 μ
M filter membranes carry out refined filtration, are passed through sterilizer 90 DEG C/min quick sterilizations, bottling sealing, the sterilization 20min in 70-72 DEG C of water, inspection
Test it is qualified by code, vanning, storage.
According to described in embodiment 2 detection method detect Resveratrol content, with simultaneously according to the methods described system of embodiment 1
The standby grape wine for obtaining is compared, and the results are shown in Table 2.
The different preparation method Resveratrol contents of table 2 compare
| Processing scheme | Trans-resveratrol mg/L | Cis-resveratrol mg/L |
| 1 | 1.91 | 0.25 |
| 2 | 1.71 | 0.18 |
Note：Processing scheme 1 is according to the methods described medicine fermentation process together with fresh grape of embodiment 1；Processing scheme 2 is
Fresh grape fermentation prepare after grape wine with Chinese medicine mixed preparing grape wine.
From the result of table 2, medicine ferments obtained grape wine Resveratrol content apparently higher than fresh together with fresh grape
With Chinese medicine mixed preparing grape wine after grape fermentation.
Embodiment 4：Different yeast prepare the influence of the Resveratrol content of grape wine to fermentation
By the methods described of embodiment 1, fermented from different saccharomycete respectively, other are operated and material mixture ratio and reality
Apply example 1 identical.According to Resveratrol content after the detection method detection fermentation described in embodiment 2,3 are the results are shown in Table.
The different yeast of table 3 prepare the influence of the Resveratrol content of grape wine to fermentation
| Group | Trans-resveratrol mg/L | Cis-resveratrol mg/L |
| Saccharomyces bayanus | 2.04 | 0.48 |
| RC212 yeast | 1.82 | 0.34 |
| F15 yeast | 1.63 | 0.18 |
From the result of table 3, according to the methods described of embodiment 1, from grape wine resveratrol prepared by saccharomyces bayanus fermentation
Content is fermented and F15 yeast apparently higher than RC212 yeast.
Embodiment 5：Different pectases prepare the influence of the Resveratrol content of grape wine to fermentation
By the methods described of embodiment 1, fermented from different pectases respectively, other are operated and material mixture ratio and reality
Apply example 1 identical.According to Resveratrol content after the detection method detection fermentation described in embodiment 2,4 are the results are shown in Table.
The different pectases of table 4 prepare the influence of the Resveratrol content of grape wine to fermentation
| Group | Trans-resveratrol mg/L | Cis-resveratrol mg/L |
| HE GRAND CRU pectases | 2.04 | 0.48 |
| HE pectases | 1.87 | 0.37 |
From the result of table 4, according to the methods described of embodiment 1, from Portugal prepared by HE GRAND CRU pectin enzyme fermentation
Grape wine Resveratrol content is apparently higher than HE pectases.
Embodiment 6：Safety evaluatio is tested
The grape wine and same batch for taking the preparation of the embodiment of the present invention 1 are not added with each 1000ml of grape wine of medicinal material fermentation, point
50ml is not concentrated under reduced pressure into, as grape wine test liquid.It is another to take cleaning grade Kunming mouse 60,20 ± 2 grams of body weight, male and female are each
Half, early daily, evening respectively gives a grape wine and grape wine concentrate, is every time 0.2ml/10g mouse weight, continuously gives 7 days, observes
Mouse outward appearance, behavior, appetite, body weight and stool and urine, as a result show that two clock test liquids do not occur anomaly, 60 mouse without
One is dead, therefore cannot ask and calculate its median lethal dose (LD50), is only capable of substantially calculating that its maximum tolerance being administered orally is 1:
20 concentration>40ml/kg body weight, it is more than 1000 times of quantity, illustrates the nontoxic pair of grape wine edible safety of the present invention
Property effect.
Embodiment 7：Mouse sperm influence experiment
Experiment packet：Commercially available three penis wine group (batch number 20100725), commercially available deer antler wine group (batch number
20100618), commercial glucose wine group (batch number 20100813), 1 group of embodiment, commercially available strength wine group (batch number
And physiological saline group 20100821).
Test method：Cleaning grade male mice in kunming 60 is taken, 20 ± 2 grams of body weight is randomly divided into 6 groups, gives respectively
Animal once a day, continuous 7 days, is taken off neck on the 8th day by above-mentioned medicinal liquor, grape wine and physiological saline, 0.1ml/10g mouse weight gavage
Vertebra is put to death, and epididymis tissue is won rapidly, after scales/electronic balance weighing, is placed in mortar and is ground, and 15 times is diluted with ringer solution, micro-
Sperm quantity is counted under mirror, and observes its activity situation, survival rate, sperm count conversion is ten thousand/10mg epididymis tissues, be the results are shown in Table
5。
The sperm of table 5 influence result of the test (N=10)
| Group | Sperm quantity (ten thousand/10mg epididymises tissue) | Living spermatozoa percentage |
| Physiological saline group | 233.1±11.5 | 58.7 ± 6.7% |
| Grape wine group | 235.3±10.1 | 64.1 ± 4.1% |
| 1 group of embodiment | 275.9±20.1 | 88.6 ± 4.5% |
| Strength wine group | 237.6±12.4 | 60.3 ± 6.7% |
| Three penis wine group | 236.9±16.3 | 64.7 ± 5.5% |
| Deer antler wine group | 247.3±13.6 | 68.6 ± 7.3% |
For male's kidney-yang deficiency, the most directly reaction is exactly the decline of sexuality, and sperm quantity and survival rate are sexualities
Important indicator, the raising of these indexs indicates the lifting of sexuality, shows there is improvement result to kidney-yang deficiency.By the knot of table 5
Really visible, gavage gives grape wine of the present invention, and mouse sperm quantity, survival rate are higher, and is substantially better than existing commercially available
Medicinal liquor and wine product group and physiological saline group, show that grape wine of the present invention can improve sexuality, to deficiency of kidney-YANG syndrome
Shape has significant curative effect.
Embodiment 8：The influence experiment of mouse sexual gland
Experiment packet：With the group technology of embodiment 4.
Test method：Cleaning grade male mice in kunming 60 is taken, 20 ± 2 grams of body weight is randomly divided into 6 groups, gives respectively
Animal once a day, continuous 7 days, is taken off neck on the 8th day by above-mentioned medicinal liquor, grape wine and physiological saline, 0.1m1/10g mouse weight gavage
Vertebra is put to death, and takes each mouse preputial gland, prostate seminal vesicle, carefully removes the accompanying fat of tissue, is sucked on surface with filter paper
Liquid, weighs on the balance of a ten thousandth, and it is average every mouse testis and epididymis tissue wet to convert, and the results are shown in Table 6.
The sexual gland of table 6 influence result of the test (N=10)
| Group | Testis weight in wet base (mg) | Epididymis tissue wet (mg) |
| Physiological saline group | 45.7±3.3 | 83.6±10.3 |
| Grape wine group | 46.1±7.5 | 87.1±11.5 |
| 1 group of embodiment | 57.5±12.4 | 113.2±12.4 |
| Strength wine group | 47.6±4.7 | 97.8±11.3 |
| Three penis wine group | 50.1±7.3 | 97.4±12.7 |
| Deer antler wine group | 48.5±6.2 | 97.8±12.3 |
The development for promoting sexual organ is a means of enhancing ability, and it is to improve male's kidney-yang deficiency that sexuality is improved
One reaction.From the result of table 6, grape wine of the present invention can increase testis and epididymis tissue wet, and substantially excellent
In existing commercially available medicinal liquor and wine product group and physiological saline group.Show that there is grape wine of the present invention androgen sample to make
With, the development of sexual organ can be promoted, there is significant curative effect to kidney-yang deficiency symptom.
Embodiment 9：To mice with kidney-yang deficiency body weight, plasma cortisol, adrenal gland seminal vesicle weights influence
Experiment packet：With the group technology of embodiment 4.
Test method：Cleaning grade male mice in kunming 120,20 grams or so of body weight, one time 0.5% hydrogenation of hypodermic injection
Cortisone 0.2m1//day, continuous 7 days.Visual animal body weight substantially mitigates around here, 21 grams or so of average out to, there is about 30%
Animal dead, the visible hair of surviving animals is loose, chilly, and limbs are sent out cool, poor appetite, the kidney-yang deficiency symptom such as dispirited.Experiment
8th day, animal is divided into 6 groups by body weight Stochastic Equilibrium, above-mentioned 4 groups of medicinal liquor, grape wine and physiological saline, 0.1ml/ are given respectively
10g mouse weight gavage, once a day, continuous 12 days, during experiment, every 4 days record body weight were once.Test the 21st day, extract mouse
Eyeball, blood is taken by orbital vein, and anticoagulant heparin after centrifugation, quantitatively draws blood plasma, by northern immunoreagent research institute method, profit
Counter is immunized with EJ-2008r, plasma cortisol measure is carried out.Compare group difference with the T methods of inspection.Take blood simultaneously
Weigh, and win bilateral adrenal glands seminal vesicle, to put and fix 24 hours in Bownis liquid, then suck surface liquid with filter paper, it is young
It is thin to remove its hetero-organization such as fat, weight in wet base is weighed on a ten thousandth balance, and it is average every mouse adrenal gland milligram to convert
Weight in wet base, statistical disposition is carried out by the method for inspection, the results are shown in Table 7.
The testing result of table 7
As shown in Table 7, grape wine of the present invention has obvious curative effects to mice with kidney-yang deficiency, can improve because of hypodermic injection hydrogen
Change body weight reduction, plasma cortisol reduction, the situation of adrenal gland weight reduction that cortisone occurs, and result is better than physiology
Salt solution group and existing commercially available medicinal liquor and wine product group.Show that grape wine of the present invention not only has and correct " deficiency of yang " mouse
Body weight and the low effect of adrenal gland endocrine function, but also with the effect for promoting " deficiency of yang " animal adrenal gland to increase weight.
Embodiment 10：Mouse mating contrast test
Experiment packet：With the group technology of embodiment 4.
Test method：Take healthy male mice in kunming 60 (20 ± 2 grams of body weight) and (mate preceding 48h with female elementization of the same race
The μ g/ of intraperitoneal injection oestradiol benzoate 20 only) female mice (20 ± 2 grams of body weight) mate 2 angels its obtain sex experience.Take out female
Mouse, makes male mouse have three days off.Male mouse is divided into 6 groups, each group gives above-mentioned 4 groups of medicinal liquor, grape wine and physiological saline respectively,
0.1ml/10g mouse weight gavage, once a day, continuous 7 days, the 8th day male mice pressed 1 with raettin:5 quantity are mated.Record its mating
Since incubation period (to the 1st time of mating of mouse mating) and 1 hour mating number of times, the results are shown in Table 8.
The mouse mating result of the test of table 8 (N=10)
| Group | Average mating incubation period (min) | Average mating number of times/hour |
| Physiological saline group | 29.8±2.9 | 4.3±0.1 |
| Grape wine group | 26.6±2.8 | 4.6±0.1 |
| 1 group of embodiment | 16.6±2.3 | 9.8±0.3 |
| Strength wine group | 26.5±2.7 | 6.1±0.2 |
| Three penis wine group | 25.8±2.9 | 5.5±0.1 |
| Deer antler wine group | 26.2±3.3 | 5.3±0.4 |
The raising of potency is to improve most directly embodying for male's kidney-yang deficiency.As seen from the results in Table 8, Portugal of the present invention
Grape wine shortens mating incubation period compared with remaining is 5 groups, increased mating number of times.Show that grape wine of the present invention can be carried
Potency high, has significant curative effect to kidney-yang deficiency symptom.
Embodiment 11：Mouse resist oxygen lack contrast test
Experiment packet：With the group technology of embodiment 4.
Test method：Healthy male mice in kunming (20 ± 2 grams of body weight) 60 is taken, 6 groups are randomly divided into, every group 10,
Each group gives above-mentioned 4 groups of medicinal liquor and physiological saline respectively, and 0.1mL/10g mouse weight gavage once a day, continuous 7 days, was filled in the 8th day
Be respectively put into the 250mL wide-mouth bottles for filling 5g soda limes each group mouse (every bottle puts 1 mouse) after 1 hour by stomach.Use all scholars
The closed bottleneck of woods, timing immediately, until breathing stops.With mouse, the time-to-live, as index, compares each group in closed wide-mouth bottle
Hypoxia endurance time, the results are shown in Table 9.
The mouse hypoxia endurance test result of table 9 (N=10)
| Group | Hypoxia endurance time (min) |
| Physiological saline group | 15.9±1.5 |
| Grape wine group | 21.1±1.3 |
| 1 group of embodiment | 23.4±1.2 |
| Strength wine group | 18.1±1.1 |
| Three penis wine group | 17.3±1.7 |
| Deer antler wine group | 18.3±1.1 |
Kidney-yang deficiency patient in addition to sexuality goes down, it is main sign be exactly fatiguability, spirit not good, hypoxia-bearing capability
Difference is to cause fatiguability, the not good main cause of spirit, thus raising mouse hypoxia-bearing capability can improve fatiguability, spirit
It is not good, so as to improve kidney-yang deficiency illness.As seen from the results in Table 9, grape wine of the present invention dramatically increases mouse hypoxia endurance time,
And better than other 5 groups, show that it has significant curative effect to fatiguability, the spirit kidney-yang deficiency symptom such as not good.
Embodiment 12：Mouse swimming exercise contrast test
Experiment packet：With the group technology of embodiment 4.
Test method：Healthy male mice in kunming (20 ± 2 grams of body weight) 60 is taken, 6 groups are randomly divided into, every group 10,
Each group gives above-mentioned 4 groups of medicinal liquor and physiological saline respectively, and 0.1mL/10g mouse weight gavage once a day, continuous 7 days, was filled in the 8th day
Stomach fastens the small iron ring of 2 grammes per square metres after 1 hour in each mouse tail line, mouse is put into the bucket of depth of water 20cm, immediately timing, until
Mouse sinks under water untill no longer upstream completely, the results are shown in Table 10.
The mouse swimming exercise result of table 10 (N=10)
| Group | Swimming time (min) |
| Physiological saline group | 19.3±1.5 |
| Grape wine group | 21.5±1.2 |
| 1 group of embodiment | 26.7±2.3 |
| Strength wine group | 19.5±1.4 |
| Three penis wine group | 19.3±2.1 |
| Deer antler wine group | 19.6±1.9 |
Sportive efforts endurance be reaction the whether vigorous important indicator of body energy, be also the middle energy of kidney-yang deficiency not
The direct performance of Ji, fatiguability, slow in reacting etc., thus improve exercise tolerance and muscle power be also kidney-yang deficiency improved it is direct
Embody.As seen from the results in Table 10, grape wine of the present invention dramatically increases mouse swimming time, and better than other 5 groups, shows it
The kidney-yang deficiency symptom such as, fatiguability not good to energy, slow in reacting has significant curative effect.
Embodiment 13：To CAMP, E2, T, Cu in kidney-yin deficiency mice plasma++、Zn++The influence of content
Experiment packet：Normal group, Kidney-yin-vacuity control group, commercially available three penis wine group (batch number:20100312)、
Commercially available deer antler wine group (batch number:20100619), commercial glucose wine group (batch number 20100813), 1 group of embodiment, commercially available
Strength wine group (batch number 20100821).
Test method：Kunming mouse 450, male and female half and half, body weight (20 ± 2) g is dynamic purchased from Medical University Of Chongqing's experiment
Thing center.Fasting 12h, free water before experiment.Laboratory temperature (24 ± 2) DEG C, humidity 60%.Model mice hypodermic injection hydrogen
Change cortisone 1mg/ only, the next day 1 time, totally 3 times, kidney-yin deficiency symptom is presented.The capacity physiological saline such as Normal group injection.Injection
2h, perfusions respectively are grouped by following afterwards.Normal group and Kidney-yin-vacuity control group (physiological saline 20ml/kg)；Grape wine
Group (20ml/kg)；1 group of embodiment (20ml/kg)；Strength wine group (20ml/kg)；Three penis wine group (20ml/kg)；Deer antler wine group
(20ml/kg).The daily gastric infusion of each group animal once, continuous 7 days.Broken end takes blood, and separated plasma uses measured by radioimmunoassay
CAMP, E2, T content, use aas determination Cu++、Zn++Content, the results are shown in Table 11.
The each group of table 11 is to CAMP, E2, T, Cu in mice plasma++、Zn++Content influence (N=10)
Note：* represent that 1 group of embodiment has significant difference P ＜ 0.05 compared with Normal group
Knowable to the result of table 11, CAMP, E2, Cu after grape wine of the present invention are taken++、Zn++Content is substantially reduced, T contents
It is significantly raised, show that grape wine of the present invention there are obvious curative effects to kidney-yin deficiency mouse, significant difference is notable.
Embodiment 14：To kidney-yin deficiency mouse phagocytosis phagocyte imdex, the influence of test for humoral immunity
Experiment packet：With the group technology of embodiment 13.
Test method：Kidney-yin deficiency mouse model is obtained as described in Example 13.
By following packet perfusions respectively.Normal group and Kidney-yin-vacuity control group (physiological saline 20ml/kg)；Grape
Wine group (20ml/kg)；1 group of embodiment (20ml/kg)；Strength wine group (20ml/kg)；Three penis wine group (20ml/kg)；Deer antler wine group
(20ml/kg).The daily gastric infusion of each group animal once, continuous 7 days.
1st, phagocytosis carbon granules experiment：Male mice 70 is taken, 7 groups are randomly divided into, every group 10, in 2h after last dose, tail
(stoste is centrifuged with preceding 2000rmp, discards sediment, and 2 are diluted with 0.9%N.S to be injected intravenously 50% india ink 0.1ml/10g
Times) 2min, 10min afterwards, blood 25ul is taken from eyeground respectively, it is dissolved in 2ml distilled water, with normal mouse blood as blank,
Absorbance (OD) is surveyed at 72 type spectrophotometer 600nm wavelength.Index K, K=1/8 × lg (OD are cleaned up in calculating2/OD10), OD2、
OD10Respectively 2min and 10min OD values.
2nd, test for humoral immunity：Male mice 70 is taken, 7 groups are randomly divided into, every group 10, mouse peritoneal on the 1st is administered
Injecting 5% sheep red blood cell (SRBC) (SRBC) 0.2ml carries out initial immunity, extracts eyeball and takes blood within 5th day after immune, separates serum.
Take 1：5000 dilute serum 1ml, 5%SRBC0.5ml, GPS 1ml, mix, and blank pipe replaces mice serum with NS,
Other are ibid.37 DEG C of water-bath 10min, 2000rmp centrifugations 10min, takes supernatant 1ml after water-bath terminating reaction, adds Dou Shi examinations
Agent 3.25ml red blood cell HD50 control tubes, take SRBC 0.25ml, add 4ml Dou Shi reagents, fully shake up.Place
10min, at 540nm, determines each pipe absorbance respectively.Calculate half hemolytic value (HC50).
Half hemolytic value=sample light absorption value/SRBC HD50 light absorption value * extension rates
Statistical analysis uses NDST, and measurement data is checked with t, and enumeration data is checked with X2, the conspicuousness between analysis each group,
And contrasted two-by-two, significance is P=0.05.Result is as shown in table 12：
Index is cleaned up after each group medication of table 12 and HD50 Data-Statistics are calculated
| Group | Dosage (ml/kg) | Clean up index (× 10-3) | HC50 |
| Normal group | 20 | 19.81±9.35 | 318.76±48.71 |
| Model group | 20 | 6.59±2.53 | 97.89±20.91 |
| Grape wine group | 20 | 15.36±5.61 | 171.37±21.45 |
| 1 group of embodiment | 20 | 22.75±11.68 | 285.61±36.39 |
| Strength wine group | 20 | 14.19±5.36 | 154.39±17.29 |
| Three penis wine group | 20 | 27.61±7.98 | 194.21±32.60 |
| Deer antler wine group | 20 | 44.78±11.34 | 255.26±47.83 |
From the result of table 12, kidney-yin deficiency mouse phagocytosis phagocyte imdex and HC50 are substantially reduced, and of the present invention
Grape wine can be remarkably reinforced kidney-yin deficiency mouse phagocyte imdex and antibody-producing capacity, than commercially available health liquor and grape wine effect
Significantly.
Embodiment 15：Preparation method of the present invention prepares grape wine
100g matrimony vines, 100g Poria cocos, 100g peanut vines, 50g sealworts, 25g Chinese cassia trees, 50g mulberry fruits are weighed respectively, crush 14
Purpose is sieved, and is fitted into good Buddhist nun's virtue fermentation tank.34Kg fresh grapes (Merlot, Cabernet Sauvignon etc.) are taken in addition, first using salicylic acid
(SA) treatment is sprayed, per kilogram grape sprays 100mlSA solution, the suitable concentration Merlot that SA is sprayed is 100mg/L, Cabernet Sauvignon
It is 60mg/L, treatment deposits 72h after darkroom；The grape after SA sprays treatment is taken out, with ultraviolet (UV) radiation-induced grape
In from the synthesis of veratryl alcohol, UV induction optimal dose Merlots are 430 seconds (1.28KJ/m2), Cabernet Sauvignon be 310 seconds (0.9KJ/
m2), treatment deposits 72h after darkroom.The grape for taking out UV irradiations is first cleaned with light water, then is cleaned with deionized water three times,
Grape harvest is by hand or destemming disintegrating machine removes carpopodium, fruit is crushed and obtains grape slurry, in the good Buddhist nun's virtue fermentation tank of addition；
Sulfurous acid 0.117% is added in broken grape slurry, makes sulfur dioxide concentration up to 0.007%, add 8.5kg sucrose, stirring is equal
It is even.Adjustment grape juice sugar content is 25%, and acid content is 0.6%, is inoculated with the saccharomyces bayanus (fermenting power (CO of 0.3wt%2) ＞
950ml moisture content ＜ 5%) and 0.3wt%HE GRAND CRU pectases (enzyme activity 400,000) and 0.3wt%-0.5wt% glucose
Glycosides enzyme (enzyme activity 100,000) is fermented, and controls fermentation temperature below 28-30 DEG C during fermentation, and stirring 2-4 times, makes daily
In floating medicinal powder and skin slag press-in grape juice；8 days after fermentation liquid residual sugars of fermentation are down to less than 0.06%, and broth temperature control exists
Room temperature, filtering wine liquid separates skin slag and obtains original wine；The sulfur dioxide that 0.3% is added in original wine carries out after fermentation, and wine is filled
Liquor storage vessel sealing preserve, at 18-25 DEG C, period carries out malic-lactic acid fermentation to temperature control as needed；Then control temperature is in 10-
15 DEG C carry out ageing, relative humidity 85%-90%, and carry out down bucket, bucketful and clarifying treatment；Determine pol, the wine of original wine
Degree, acidity, being adjusted to setting respectively according to the physical and chemical index of finished product carries out blending treatment, stores 28 days；Wine liquid is crossed into 0.45 μ
M filter membranes carry out refined filtration, are passed through sterilizer 90 DEG C/min quick sterilizations, bottling sealing, the sterilization 20min in 70-72 DEG C of water, inspection
Test it is qualified by code, vanning, storage.
Carry out coherent detection according to embodiment 2, the method for embodiment 6-14, grape wine manufactured in the present embodiment to impotence,
The kidney-yang deficiency symptom such as hypogona dism, fatiguability, hypoimmunity, energy is not good and there is significant curative effect, effect is better than existing guarantor
Strong product.
Embodiment 16：Preparation method of the present invention prepares grape wine
60g matrimony vines, 40g Poria cocos, 80g peanut vines, 50g sealworts, 25g Chinese cassia trees, 45g mulberry fruits are weighed respectively, crush 14 mesh
Sieve, be fitted into good Buddhist nun's virtue fermentation tank.
20Kg fresh grapes (Merlot, Cabernet Sauvignon etc.) are taken in addition, and treatment, per kilogram grape are first sprayed using salicylic acid (SA)
100mlSA solution is sprayed, the suitable concentration Merlot that SA is sprayed is 100mg/L, Cabernet Sauvignon is 60mg/L, and treatment is deposited after darkroom
Put 72h；The grape after SA sprays treatment is taken out, with the synthesis in ultraviolet (UV) radiation-induced grape from veratryl alcohol, UV inductions
Optimal dose Merlot is 430 seconds (1.28KJ/m2), Cabernet Sauvignon be 310 seconds (0.9KJ/m2), treatment deposits 72h after darkroom.
The grape of UV irradiations is taken out, is first cleaned with light water, then cleaned with deionized water three times, grape harvest is by hand or destemming disintegrating machine
Removal carpopodium, fruit is crushed and obtains grape slurry, in the good Buddhist nun's virtue fermentation tank of addition.
Sulfurous acid 0.066% is added in broken grape slurry, makes sulfur dioxide concentration up to 0.004%, add 4kg sugarcanes
Sugar, stirs.Adjustment grape juice sugar content is 20%, and acid content is 0.6%, is inoculated with the saccharomyces bayanus (fermenting power of 0.5wt%
(CO2) ＞ 950ml moisture content ＜ 5%) and 0.5wt%HE GRAND CRU pectases (enzyme activity 400,000) and 0.5wt%-
0.5wt% glucuroides (enzyme activity 100,000) are fermented, and fermentation temperature is controlled during fermentation below 28-30 DEG C, daily
Stirring 2-4 times, makes the medicinal powder of floating and skin slag be pressed into grape juice；The 9 angel's zymotic fluid residual sugars that ferment are down to less than 0.06%, hair
Zymotic fluid temperature control separates skin slag and obtains original wine in room temperature, filtering wine liquid；The sulfur dioxide that 0.3% is added in original wine is carried out
After fermentation, wine fills liquor storage vessel sealing preserve, and at 18-25 DEG C, period carries out malic-lactic acid fermentation to temperature control as needed；Then
Control temperature carries out ageing, relative humidity 85%-90% at 10-15 DEG C, and carries out down bucket, bucketful and clarifying treatment；Determine former
The pol of wine liquid, wine degree, acidity, being adjusted to setting respectively according to the physical and chemical index of finished product carries out blending treatment, stores 28 days；
Wine liquid is crossed into 0.45 μm of filter membrane carries out refined filtration, is passed through sterilizer 90 DEG C/min quick sterilizations, bottling sealing, in 70-72 DEG C of water
Middle sterilized 20min, inspection is qualified can code, vanning, storage.
Carry out coherent detection according to embodiment 2, the method for embodiment 6-14, grape wine manufactured in the present embodiment to impotence,
Hypogona dism, fatiguability, hypoimmunity, energy is not good etc., and kidney-yang deficiency symptom has significant curative effect, and effect is better than existing health care
Product.
Embodiment 17：Preparation method of the present invention prepares grape wine
100g matrimony vines, 75g Poria cocos, 80g peanut vines, 70g sealworts, 35g Chinese cassia trees, 60g mulberry fruits are weighed respectively, crush 14 mesh
Sieve, be fitted into good Buddhist nun's virtue fermentation tank.
24Kg fresh grapes (Merlot, Cabernet Sauvignon etc.) are taken in addition, and treatment, per kilogram grape are first sprayed using salicylic acid (SA)
100mlSA solution is sprayed, the suitable concentration Merlot that SA is sprayed is 100mg/L, Cabernet Sauvignon is 60mg/L, and treatment is deposited after darkroom
Put 72h；The grape after SA sprays treatment is taken out, with the synthesis in ultraviolet (UV) radiation-induced grape from veratryl alcohol, UV inductions
Optimal dose Merlot is 430 seconds (1.28KJ/m2), Cabernet Sauvignon be 310 seconds (0.9KJ/m2), treatment deposits 72h after darkroom.
The grape of UV irradiations is taken out, is first cleaned with light water, then cleaned with deionized water three times, grape harvest is by hand or destemming disintegrating machine
Removal carpopodium, fruit is crushed and obtains grape slurry, in the good Buddhist nun's virtue fermentation tank of addition.
Sulfurous acid 0.1% is added in broken grape slurry, makes sulfur dioxide concentration up to 0.006%, add 5.2kg sugarcanes
Sugar, stirs.Adjustment grape juice sugar content is 22%, and acid content is 0.6%, is inoculated with the saccharomyces bayanus (fermenting power of 0.3wt%
(CO2) ＞ 950ml moisture content ＜ 5%) and 0.3wt%HE GRAND CRU pectases (enzyme activity 400,000) and 0.3wt%-
0.5wt% glucuroides (enzyme activity 100,000) are fermented, fermentation temperature is controlled during fermentation below 28-30 DEG C, daily
Stirring 2-4 times, makes the medicinal powder of floating and skin slag be pressed into grape juice；9 days zymotic fluid residual sugars of fermentation are down to less than 0.06%, fermentation
Liquid temperature control separates skin slag and obtains original wine in room temperature, filtering wine liquid；After 0.3% sulfur dioxide is added in original wine carrying out
Fermentation, wine fills liquor storage vessel sealing preserve, and at 18-25 DEG C, period carries out malic-lactic acid fermentation to temperature control as needed；Then control
Temperature processed carries out ageing, relative humidity 85%-90% at 10-15 DEG C, and carries out down bucket, bucketful and clarifying treatment；Determine former wine
The pol of liquid, wine degree, acidity, being adjusted to setting respectively according to the physical and chemical index of finished product carries out blending treatment, stores 28 days；Will
Wine liquid crosses 0.45 μm of filter membrane and carries out refined filtration, is passed through sterilizer 90 DEG C/min quick sterilizations, bottling sealing, in 70-72 DEG C of water
Sterilized 20min, inspection is qualified can code, vanning, storage.
Carry out coherent detection according to embodiment 2, the method for embodiment 6-14, grape wine manufactured in the present embodiment to impotence,
Hypogona dism, fatiguability, hypoimmunity, energy is not good etc., and kidney-yang deficiency symptom has significant curative effect, and effect is better than existing health care
Product.
Embodiment 18：Preparation method of the present invention prepares grape wine
160g matrimony vines, 110g Poria cocos, 130g peanut vines, 120g sealworts, 60g Chinese cassia trees, 120g mulberry fruits are weighed respectively, are crushed
The sieve of 14 mesh, is fitted into good Buddhist nun's virtue fermentation tank.
56Kg fresh grapes (Merlot, Cabernet Sauvignon etc.) are taken in addition, and treatment, per kilogram grape are first sprayed using salicylic acid (SA)
100mlSA solution is sprayed, the suitable concentration Merlot that SA is sprayed is 100mg/L, Cabernet Sauvignon is 60mg/L, and treatment is deposited after darkroom
Put 72h；The grape after SA sprays treatment is taken out, with the synthesis in ultraviolet (UV) radiation-induced grape from veratryl alcohol, UV inductions
Optimal dose Merlot is 430 seconds (1.28KJ/m2), Cabernet Sauvignon be 310 seconds (0.9KJ/m2), treatment deposits 72h after darkroom.
The grape of UV irradiations is taken out, is first cleaned with light water, then cleaned with deionized water three times, grape harvest is by hand or destemming disintegrating machine
Removal carpopodium, fruit is crushed and obtains grape slurry, in the good Buddhist nun's virtue fermentation tank of addition.
Sulfurous acid 0.066% is added in broken grape slurry, makes sulfur dioxide concentration up to 0.004%, add 10.08kg
Sucrose, stirs.Adjustment grape juice sugar content is 18%, and acid content is 0.6%, is inoculated with the saccharomyces bayanus (fermenting power of 0.3wt%
(CO2) ＞ 950ml moisture content ＜ 5%) and 0.3wt%HE GRAND CRU pectases (enzyme activity 400,000) and 0.3wt%-
0.5wt% glucuroides (enzyme activity 100,000) are fermented, fermentation temperature is controlled during fermentation below 28-30 DEG C, daily
Stirring 2-4 times, makes the medicinal powder of floating and skin slag be pressed into grape juice；9 days after fermentation liquid residual sugars of fermentation are down to less than 0.06%, hair
Zymotic fluid temperature control separates skin slag and obtains original wine in room temperature, filtering wine liquid；The sulfur dioxide that 0.3% is added in original wine is carried out
After fermentation, wine fills liquor storage vessel sealing preserve, and at 18-25 DEG C, period carries out malic-lactic acid fermentation to temperature control as needed；Then
Control temperature carries out ageing, relative humidity 85%-90% at 10-15 DEG C, and carries out down bucket, bucketful and clarifying treatment；Determine former
The pol of wine liquid, wine degree, acidity, being adjusted to setting respectively according to the physical and chemical index of finished product carries out blending treatment, stores 28 days；
Wine liquid is crossed into 0.45 μm of filter membrane carries out refined filtration, is passed through sterilizer 90 DEG C/min quick sterilizations, bottling sealing, in 70-72 DEG C of water
Middle sterilized 20min, inspection is qualified can code, vanning, storage.
Carry out coherent detection according to embodiment 2, the method for embodiment 6-14, grape wine manufactured in the present embodiment to impotence,
Hypogona dism, fatiguability, hypoimmunity, energy is not good etc., and kidney-yang deficiency symptom has significant curative effect, and effect is better than existing health care
Product.
Embodiment 19：Clinical test
1st, slowly certain, man, 40 years old, usually feel be short of physical strength, impotence lift, shortness of breath, manual labor lethargy.Through tcm diagnosis
It is kidney deficiency.Grape wine described in drinking the embodiment of the present invention 1 is taken good care of and is treated, and 50 milliliters is drunk after daily supper, continuously
Drink 3 months, muscle power is restored, energetic, impotence symptom disappears, and supports and hard.Think after TCM pulse diagnosis, its kidney work(
Can reach as normal person.Confirm that its illness obtains medical treatment, muscle power is restored with energy.Prove grape wine of the present invention
It is effective to kidney deficiency.
2nd, Lee, man 38 years old, feels dampness of scrotum, and body void hidrosis, erection is unfavorable.Think through tcm diagnosis, kidney yang is not
Foot.Grape wine described in drinking the embodiment of the present invention 1 is nursed one's health, and one time a day, 80 milliliters every time, is drunk after supper.Through drinking 45
My god, treat shape and disappear.Scrotum is no longer moist, vigorous generation rapidly with it is strong, feel feel full of physical strength.Prove grape wine of the present invention
Generate the effect for the treatment of and regulation.
3rd, Zhu, man 47 years old, feels weak all over, shortness of breath, night sweat.Think through tcm diagnosis, caused by kidney deficiency.Drink this
Grape wine described in inventive embodiments 1 is taken good care of.50 milliliters are drunk every time, 1 time is respectively drunk after daily supper and before sleep, daily
Drink with 100 milliliters.After being drunk through 2 months, from head to foot effectively, shortness of breath phenomenon disappear, night sweat stop to.Prove grape of the present invention
Wine generates the effect of conditioning.
4th, Lee, man 52 years old, feels thanks to gas, head fan, impotence, small energy having sexual intercourse thing.Think through tcm diagnosis, serious kidney deficiency
Held back.Grape wine described in drinking the embodiment of the present invention 1 is treated and nursed one's health.50 milliliters are drunk daily, is drunk after supper, drunk
After 3 months, feel and no longer lose gas, lung capacity increases, and head fan symptom disappears, no longer impotence, and during having sexual intercourse, sun tool is bold
Effectively, it was demonstrated that grape wine of the present invention generates treatment and opsonic action.
5th, money, man, 56 years old, having sexual intercourse was difficult, sometimes impotence, and premature ejaculation, perplexs its normal life often sometimes.Examined through the traditional Chinese medical science
It is disconnected to think the insufficiency of the kidney yang, sexual disorder, it is proposed that to be treated with kidney tonifying method.Drink grape wine described in the embodiment of the present invention 15
Daily 60 milliliters, after drinking 2 months, doctor explains the situation in whereabouts with a smile to shut out certain, says having sexual intercourse such as young man, and mood is pleased
It is happy.Prove that grape wine of the present invention weakens effect to treatment man sexual function obvious.
6th, Zhao, man, 63 years old, suffers from sex dysfunction in 2006, with kidney deficiency, soreness and weakness of waist and knees illness, is imitated through hospitalize
Fruit is not good, drinks this grape wine since in the October, 2008, and the state of an illness gradually takes a turn for the better after half a year, hereafter adheres to drinking of the invention real every night
50 milliliters of grape wine described in example 16 are applied, so far more than 4 years, has not only cured the diseases such as kidney deficiency and sex dysfunction, and appearance is not
Always, it is hale and hearty, it appears that as 50 people how old.
7th, Lee, man 42 years old, suffers from night sweat, lumbago due to the kidney deficiency, the not normal illness of sexual function, controls small more long, when it is good when it is bad, take
Grape wine is clearly better after 3 months described in the embodiment of the present invention 16, is returned to one's perfect health after half a year, still is adhering to taking at present, constitution
Become better and better.
8th, river, man, 49 years old, suffers from kidney deficiency and liver, waist and knee weakness, blockage of main and collateral channels illness.It is in poor health, lassitude, warp
Multi-party treatment curative effect is little.In May, 2009 starts to take grape wine described in the embodiment of the present invention 17, and October, sb.'s illness took a favorable turn,
The first half of the year in 2010 is almost recovered, and clothes are to present, and health is good.
9th, certain is shut out, man, 60 years old, after patient 2008 tumbles by motorcycle, waist bar was sprained and fully not recovered always, often existing pain in the back,
In November, 2010 finds that tumble injury position goes out the obvious osteoproliferation of item, and its winter trick is ice-cold, and chilly is cold, waist sleepiness power, insomnia
Dreaminess, impotence is not lifted.August in 2011 26 rise start to take the embodiment of the present invention 17 described in grape wine, taken during supper every night
70 milliliters, pain in the back substantially mitigates after taking medicine continuously 15 days, continuously takes later 45 days, and pain in the back is wholly absent, and goes hospital's photo to find bone
Matter hyperplasia is substantially eliminated, and sleep is good and dream is few, and trick is warmed up, and body is strengthened, energetic, and sun tool is sturdy strong such as 30 years old young man.
10th, Zheng, female 52 years old, suffers from rheumatism, rheumatoid, arthritis, whenever rain, when the change of weather all can knee pain,
It is little through multi-party treatment curative effect.In October, 2010 starts to take grape wine described in the embodiment of the present invention 18, even drink 15 days, pain portion
There is substantially mitigation position, continuously drinks 2 months, and disease sites are without obvious pain during drinking.
11st, Huang, female 47 years old, suffers from frequent micturition, urgent urination, the symptom such as frequently get up in the night to urinate, and takes grape described in the embodiment of the present invention 18
Wine 7-15 days, frequency of getting up in the night to urinate is significantly reduced, and urine urgency-frequency is also improved, even drink 1-2 month, without symptom of getting up in the night to urinate, after stopping drink
Also do not rebound.
12nd, Dai, female 36 years old, suffers from soreness and weakness of waist and knees, two skelasthenias, and dizziness and tinnitus, insomnia and dreamful sleep is originally thought that oneself is each
Square pressure is caused greatly, is kidney-yin deficiency through tcm diagnosis.Grape wine 15-25 days described in the embodiment of the present invention 18 are taken, constitution is bright
Aobvious to improve, sleep quality is significantly improved, even drink more than 2 months, and illness does not occur substantially, stops the symptom that also do not rebounded during drink
Occur.
13rd, for certain, female, 28 years old, patient was through less, body is become thin, hectic fever night sweat.Take grape described in the embodiment of the present invention 18
Wine -1 month 15 days (being disabled during menstrual period), symptom is had clear improvement, even drink 3 months, occurs without illness, and constitution is also obtained substantially
Improve.
The explanation of above example is only intended to help and understands the method for the present invention and its core concept.It should be pointed out that right
For those skilled in the art, under the premise without departing from the principles of the invention, the present invention can also be carried out
Some improvement and modification, these are improved and modification is also fallen into the protection domain of the claims in the present invention.
Claims (9)
1. a kind of grape wine, is made up of following raw material：
50 parts -100 parts of matrimony vine, 40 parts -100 parts of Poria cocos, 50 parts -100 parts of peanut vine, 50 parts -70 parts of sealwort, 25 part -50 of Chinese cassia tree
Part, 45 parts -60 parts of mulberry fruit, 20000 parts -34000 parts of grape；
The grape wine is prepared by the following method：
By the pretreated grape of the weight portion of 20000 weight portion -34000 and the weight portion matrimony vine of 50 weight portion -100,40 weight portions -
100 weight portion Poria cocos, the weight portion peanut vine of 50 weight portion -100, the weight portion sealwort of 50 weight portion -70, the weight of 25 weight portion -50
Part Chinese cassia tree, the weight portion mulberry fruit of 45 weight portion -60 are mixed into row alcoholic fermentation, after allocated after after fermentation, ageing；
The pretreatment is that the synthesis after treatment with resveratrol in ultraviolet radiation-induced grape is sprayed using salicylic acid；It is described
SA concentration is 60mg/L-100mg/L, the UV inducing amount 0.9KJ/m2-1.28KJ/m2, -430 seconds 310 seconds；
The alcoholic fermentation is 18%-25% for adjustment grape juice sugar content, and acid content is 0.6%-0.8%, inoculation
The yeast of 0.3wt%-0.5wt%, 0.3wt%-0.5wt% pectases and 0.3wt%-0.5wt% glucuroides are sent out
Ferment, controls fermentation temperature at 28-30 DEG C during fermentation, fermentation to zymotic fluid residual sugar is down to less than 0.06%.
2. a kind of preparation of wine as claimed in claim 1, preprocessed by the weight portion of 20000 weight portion -34000
Grape and the weight portion matrimony vine of 50 weight portion -100, the weight portion Poria cocos of 40 weight portion -100, the weight portion peanut of 50 weight portion -100
Rattan, the weight portion sealwort of 50 weight portion -70, the weight portion Chinese cassia tree of 25 weight portion -50, the mixing of the weight portion mulberry fruit of 45 weight portion -60 are carried out
Alcoholic fermentation, after after after fermentation, ageing allocate.
3. preparation method according to claim 2, the grape is Merlot, Cabernet Sauvignon.
4. preparation method according to claim 2, the pretreatment is to be shone with ultraviolet after spraying treatment using salicylic acid
Penetrate the synthesis of resveratrol in induction grape.
5. preparation method according to claim 4, the SA concentration is 60mg/L-100mg/L, the UV inducing amount
0.9KJ/m2-1.28KJ/m2, -430 seconds 310 seconds.
6. preparation method according to claim 2, the alcoholic fermentation is 18%-25% for adjustment grape juice sugar content,
Acid content is 0.6%-0.8%, is inoculated with yeast, 0.3wt%-0.5wt% pectases and the 0.3wt%- of 0.3wt%-0.5wt%
0.5wt% glucuroides are fermented, and control fermentation temperature at 28-30 DEG C during fermentation, and fermentation to zymotic fluid residual sugar is down to
Less than 0.06%.
7. preparation method according to claim 6, the yeast is saccharomyces bayanus.
8. preparation method according to claim 6, the pectase is HE GRAND CRU pectases.
9. grape wine described in claim 1 prepare treatment kidney deficiency medicine in purposes.
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|---|---|---|---|---|
| CN1203939A (en) * | 1998-06-16 | 1999-01-06 | 宁夏农林科学院农副产品贮藏加工研究所 | Lycium berry and grape mix-fermented wine and its brewing method |
| CN1111596C (en) * | 2000-01-08 | 2003-06-18 | 宁夏玉泉葡萄酿酒研究所 | Brewage of Lycium Chinense-grape wine |
| CN101177659A (en) * | 2006-11-09 | 2008-05-14 | 天津科技大学 | Preparation technology of dry red wine with high content of resveratrol |
| WO2009089334A1 (en) * | 2008-01-08 | 2009-07-16 | David Rubin | Method and compositions for preserving wine |
| CN101591597A (en) * | 2008-05-31 | 2009-12-02 | 李振香 | A Liver and Kidney Wine |
| CN102115707B (en) * | 2010-12-20 | 2012-08-15 | 重庆市科学技术研究院 | Health wine and preparation process thereof |
| CN102703271A (en) * | 2012-06-15 | 2012-10-03 | 徐州大丰食品有限公司 | Formula of grape medicinal liquor and preparation method thereof |
| CN103735838B (en) * | 2014-01-14 | 2016-05-04 | 重庆利莫里亚生物科技有限公司 | One breeding kidney health care medicinal liquor for tonifying kidney and preparation method thereof |
-
2014
- 2014-12-15 CN CN201410776047.7A patent/CN104388236B/en active Active
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