CN104367601A - Compound medicine containing herba pteridis multifidae and mequindox for livestock and poultry - Google Patents

Abstract

The invention relates to a compound medicine containing herba pteridis multifidae and mequindox for livestock and poultry. The compound medicine comprises the following raw materials in parts by weight: 3-30 parts of mequindox and 5-200 parts of herba pteridis multifidae. After herba pteridis multifidae is added to mequindox, the drug resistance of bacteria on the mequindox can be reversed, thus the treatment effect of the mequindox is improved. The compound medicine is prepared into powder, tablets, oral liquid or granules according to the conventional method; and 0.1-7g of compound medicine is added to each kg of feed for livestock and poultry for feeding, so that the medication cost and the drug residues can be greatly lowered; relatively high economic benefits are created for farms; and meanwhile, the food safety is also ensured.

Description

A kind of compound medicine for livestock and poultry containing Pteris vulgaris and Shigella

technical field

The invention belongs to the technical field of treatment of Escherichia coli and Salmonella infection in livestock and poultry, and in particular relates to a compound medicine for livestock and poultry composed of Pteridaceae Pteridaceae plants Pteris chinensis and dysentery.

Background technique

Avian salmonellosis is a general term for poultry diseases caused by one or more Salmonella in the genus Salmonella. According to the different antigenic structures of pathogens, they can be divided into three categories: pullorum, avian typhoid and avian paratyphoid. Among them, Pullorum pullorum and Salmonella typhi have host specificity, mainly causing disease in chickens and turkeys; Salmonella avian paratyphi can widely infect a variety of animals and humans, and poultry and their products contaminated by it are human Salmonella infection and food poisoning one of the main sources of . Avian salmonellosis often forms a rather complex cycle of transmission. Sick poultry and infected poultry are the main sources of infection, which can be transmitted horizontally through the digestive tract, respiratory tract or conjunctiva, and can infect young poultry after vertical transmission through eggs, causing most of them to die. Use this as a breeding egg to hatch, then it can be passed on from generation to generation again and again, causing poultry salmonellosis to be difficult to purify in flocks, causing serious economic losses. Because Salmonella has a large pollution area in chicken flocks and is prone to drug resistance, the vaccines currently on the market have unsatisfactory immune effects. Therefore, quarantine of breeder flocks, drug control, environmental disinfection and purification, strengthening feeding management and sanitation and epidemic prevention are still fundamental measures to control and prevent Salmonella from entering the food chain. After hatching, the chicks drink 0.01% potassium permanganate solution or other drugs for 1 to 2 days for drug prevention. The bacteria are resistant to commonly used drugs, forcing the production of various drugs to be used alternately in order to better control drug-resistant bacteria. At present, the bacteria have strong drug resistance to aminoglycoside drugs. Aminoglycoside inactivating enzymes modify some groups necessary to protect antibacterial activity in these antibacterial drug molecules, so that they can interact with the target ribosome affinity is greatly reduced. These inactivating enzymes include aminoglycoside acyltransferase (AAC), aminoglycoside adenylyltransferase (AAD), aminoglycoside phosphotransferase (APH), adenosylnucleosyltransferase (ANT), and the like. At present, inactivating enzymes with different characteristics have been found in many aminoglycoside-resistant bacteria.

Escherichia coli is a common conditional pathogen and a common disease. It can cause acute sepsis, peritonitis, hepatitis, pneumonia, enteritis and other severe infections in chickens, and can also be secondary or mixed with viral diseases. , causing huge economic losses to the farming industry. Amoxicillin, ampicillin, ceftiofur, ceftriaxone, amoxicillin/clavulanic acid, ampicillin/sulbactam and other β-lactam ring antibiotics are commonly used important drugs for the prevention and treatment of this disease. The degree of scale intensification is increasing, and the use of drugs is becoming more and more widespread. There are more and more drug-resistant strains, and there are more reports on the drug resistance of E. coli. Studies have confirmed that one of the main mechanisms of drug resistance is the production of β-lactamase, especially extended-spectrum β-lactamase (Extended-Spectrum β-lactamase, ESBLs), which can hydrolyze β-lactamase strongly and rapidly. The lactam ring makes these antibiotics lose their antibacterial activity. ESBLs are the most important enzyme type of β-lactamases. It is not only resistant to third-generation cephalosporins and aztreonam, but also cross-resistant to aminoglycosides, quinolones, and sulfonamides. It is only resistant to carbapenems, cephalosporins, and Sensitivity to mycophenes. Many literatures have reported that the multidrug resistance of Escherichia coli is closely related to the production of ESBLs, which has attracted more attention at home and abroad. The inventor has recently carried out studies on the detection and extraction of food animal-derived pathogenic bacteria ESBLs, the hydrolysis rate of enzymes on antibiotics, and the enzyme-inhibiting protective effects of sulbactam, tazobactam and traditional Chinese medicines. The drug sensitivity of some ESBLs Escherichia coli Tests showed that its multi-drug resistance rate was significantly higher than that of non-ESBLs strains. In addition, the results also showed that ESBLs-producing strains were not only severely resistant to third-generation cephalosporins such as ceftiofur, but also resistant to fluoroquinolones, aminoglycosides, sulfonamides, and fosfomycin. Severe multidrug resistance. The inventor isolated fosfomycin-resistant Escherichia coli producing the fosA3 drug-resistant gene from ESBLs-producing strains, which further enhanced the drug resistance of the bacteria.

The drug resistance of Salmonella and Escherichia coli increases the cost of medication for farmers, prolongs the course of treatment, increases the mortality of livestock and poultry, brings greater economic losses to production, and increases the risk of drug residues in livestock and poultry. Therefore, in actual production, there is an urgent need for a drug that can have a better therapeutic effect on Salmonella and Escherichia coli no matter whether it is drug-resistant or not, and can shorten the course of treatment, reduce mortality, reduce economic losses and reduce drug residues. Create higher economic benefits and protect human health and safety.

Contents of the invention

The technical problem to be solved by the present invention is to provide a compound drug for livestock and poultry containing Pteridaceae Pteridaceae Pteridaceae Pteridaceae and Shigaojing, which can kill bacteria faster and overcome the problem of antibiotic resistance caused by antibacterial treatment The defects of poor curative effect, large dosage, high residue and high cost avoid the problems of narrow bactericidal spectrum and slow bactericidal effect of simple traditional Chinese medicine.

In order to solve the above technical problems, the present invention adopts the following technical scheme: a compound medicine for livestock and poultry containing Pteridaceae Pteridaceae Pteridaceae Pteridaceae and Dysentery, consisting of the following raw materials in parts by weight: 3-30 parts of Dysentery, Pteris 5-200 parts.

The compound medicine is prepared into powder, tablet, oral liquid or granule.

The preparation method of the compound medicine is as follows: pulverize the Pteris fragrans through a 60-mesh sieve, then mix it with the powdered antibacterial drug, and prepare it into powder, tablet, oral liquid or granule according to the conventional method.

Aiming at the problem of drug resistance existing in Escherichia coli and Salmonella infection in livestock and poultry at present, the inventor uses Pteridaceae Pteris and antibacterial drugs to form a compound drug. Studies have shown that adding Pteris to antibacterial drugs can reverse the resistance of bacteria to antibacterial drugs, thereby improving the therapeutic effect of antibacterial drugs. The compound drug is made into powder, tablet, oral liquid or granule according to conventional methods, and added to livestock and poultry feed according to the addition amount of 0.1-7g main drug/kg feed, which can greatly reduce the cost of drug use and drug residues , to create higher economic benefits for farmers, but also to ensure food safety.

Detailed ways

1. Antimicrobial resistance inhibition test of Escherichia coli after Pteris vulgaris and antibacterial drugs were mixed

Antibacterial drugs themselves have the effect of treating Escherichia coli and Salmonella, but due to the strong drug resistance of Escherichia coli and Salmonella producing drug resistance genes, antibacterial drugs do not show a good therapeutic effect under normal dosage conditions .

The minimum inhibitory concentration of antibacterial drugs against Escherichia coli strains producing ESBLs and fosA3 resistance genes and the effect of Pteris vulgaris on Escherichia coli strains producing ESBLs and fosA3 resistance genes were determined on a 96-well plate The sub-inhibitory concentration of the sub-inhibitory concentration, and then add the sub-inhibitory concentration of Pteris vulgaris in the medium, and then use the micro test tube double dilution method to cultivate and determine the minimum inhibitory concentration of the antibacterial drug; use the continuous passage method to add the same sub-inhibitory concentration Subculture Escherichia coli strains producing ESBLs and fosA3 drug-resistant genes in the medium of Pteris vulgaris and the same concentration of antibacterial drugs until the observed minimum inhibitory concentration of antibacterial drugs is more than 4 times smaller than that before subculture. It is judged that Pteris vulgaris has drug resistance reversal effect on antibacterial drugs. The statistics of the test results are shown in Table 1:

Table 1 The sub-inhibitory concentration (0.5g/mL) of sub-inhibitory concentration (0.5g/mL) Pteris chinensis subculture caused the change of MIC of western medicine of ESBLs and fosA3 Escherichia coli (μg/mL).

Two, the application example of compound medicine of the present invention

Prepare Example 1-Example 4 according to the formula in Table 2 and the following methods: after the Pteris praecox is crushed through a 60-mesh sieve, as the basic raw material, put it into a mixer in proportion by weight with the powdered antibacterial drug, and mix evenly. The compound medicine is prepared into a preparation product.

The formula of table 2 embodiment 1-4 and preparation dosage form

serial number Antibacterials Pteris dosage form

Example 1 Shigella net 11g 5g oral liquid Example 2 Shigella net 20g 10g Granules Example 3 Shigella net 3g 45g Powder Example 4 Shigella net 30g 180g tablet

1. The curative effect experiment of the compound drug product of embodiment 1-4 gained to the piglet diarrhea of 33 days of age

Clinical symptoms: 33-day-old piglets in a pig farm in Fangchenggang, Guangxi. After weaning and turning around, the pigs began to have diarrhea and pull watery feces. The feces contained undigested feed. The pigs ate the feed but did not grow. Diarrhea, dehydration and death, no symptoms of vomiting, the farmers used a variety of drugs, but the treatment did not have the desired effect.

Necropsy lesions: After necropsy, the intestinal wall of the pig is thin, especially in the rear end of the small intestine and the colon, the intestinal mucosa is shed, and the intestinal wall is congested and bleeding.

Laboratory Diagnosis: Stained slices of the lesioned liver were taken, Gram stained, observed under a microscope, and red Gram-negative bacilli were seen; it was preliminarily determined to be Escherichia coli disease. Through Escherichia coli culture and biochemical test identification, it was judged that the piglets were infected with Escherichia coli disease, and PCR amplification proved that there were CTX-M type ESBLs drug resistance gene and fosA3 drug resistance gene.

Experimental drug regimen: the diseased pigs were divided into 6 groups for administration, the first group was administered according to the addition amount of 0.3g/kg feed in accordance with Example 1, and the second group was administered according to the addition amount of 0.3g/kg feed in Example 2 , the third group is administered according to the addition amount of 3g/kg feed with embodiment 3, and the fourth group is administered according to the addition amount of 1.4g/kg feed with embodiment 4; The addition amount of kg feed was administered, and the sixth group was administered with Pteris vulgaris coarse powder according to the addition amount of 4g/kg feed; the above six groups of medication schemes were used continuously for 5 days.

Feedback on the use effect: 2 days after the first group to the fourth group of medication, the pigs no longer pull watery feces, the feces are formed, and the feces no longer contain feed. Continue to use for 3 days, and the pigs are cured; 2 days after the fifth group of medication, The pigs are still unhealthy, continue to use for 3 days, and basically recover to health, but some still have mushy feces, but after 3 days of stopping the drug, the pigs relapse; 2 days after the sixth group of drugs, there is no obvious improvement in the pigs, continue to use for 3 days , the pigs continued to have diarrhea, and the rest were more serious. It can be seen that the curative effect of the compound composition containing Pteris vulgaris on swine is better than that of unilateral dysentery and unilateral Pteris vulgaris.

2. The curative effect experiment of the compound drug product obtained in Examples 1-4 to chicken salmonella disease.

Clinical symptoms: In a chicken farm in Nanning, Guangxi, 14-day-old broiler chickens had diarrhea, white, pasty and loose feces, the fluff around the anus was contaminated by feces, there were lime-like lumps, and some screamed in pain. death phenomenon. Autopsy of individual chicken liver enlargement is dark red to dark purple, or slightly khaki, crisp and easy to break, the surface is scattered or densely covered with grayish-white, grayish-yellow necrotic spots, sometimes red bleeding spots, typical characteristics of salmonellosis.

Laboratory Diagnosis: Stained slices of the lesioned liver were taken, Gram stained, observed under a microscope, and red Gram-negative bacilli were seen; it was preliminarily judged to be Salmonella disease. Through Salmonella culture and biochemical test identification, it was judged that the chicken infection was Salmonella disease, and the results of the drug susceptibility test were judged to be drug-resistant Salmonella strains according to the NCCLS standard.

Experimental medication regimen: the diseased chickens were divided into 6 groups for administration, the first group was administered according to the addition amount of 0.3g/kg feed with Embodiment 1, and the second group was administered according to the addition amount of 0.3g/kg feed with Embodiment 2. Medicine, the third group is administered according to the addition amount of 3g/kg feed with embodiment 3, and the fourth group is administered according to the addition amount of 1.4g/kg feed with embodiment 4; The addition amount of /kg feed was administered, and the sixth group was administered with Pteris vulgaris coarse powder according to the addition amount of 4g/kg feed; the above six groups of medication schemes were used continuously for 5 days.

Effect feedback: After the first group to the fourth group of chickens took medicine, the feed intake of the diseased chickens increased, and no dead chickens appeared. After 3 days of continuous use, the chickens were basically cured; Most of the flocks are still unhealthy, and some chickens still die. Continue to use for 3 days, and the death of chickens has not been effectively controlled; after 2 days of medication, the chickens in the sixth group have not changed significantly. Continue to use for 3 days, the chickens The death phenomenon has not been effectively controlled. It can be seen that the curative effect of the compound medicine containing Pteris vulgaris is better than that of the single prescription.

Claims (2)

1. contain a poultry compound medicine for Pteridaceae Herba Pteridis Multifidae and LIJUNJING, it is characterized in that, be made up of the raw material of following weight portion: LIJUNJING 3-30 part, Herba Pteridis Multifidae 5-200 part.

2. the poultry compound medicine containing Pteridaceae Herba Pteridis Multifidae and LIJUNJING according to claim 1, it is characterized in that, described compound medicine is prepared into powder, tablet, oral liquid or granule.