CN104365540A - Quasipaa spinosa induced spawning and efficient breeding method - Google Patents
Quasipaa spinosa induced spawning and efficient breeding method Download PDFInfo
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- CN104365540A CN104365540A CN201410572372.1A CN201410572372A CN104365540A CN 104365540 A CN104365540 A CN 104365540A CN 201410572372 A CN201410572372 A CN 201410572372A CN 104365540 A CN104365540 A CN 104365540A
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- Prior art keywords
- frog
- pond
- straw mat
- stone
- ovum
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- Pending
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 19
- 241001560470 Quasipaa spinosa Species 0.000 title abstract description 7
- 239000010902 straw Substances 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 20
- 235000013601 eggs Nutrition 0.000 claims abstract description 17
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 108700012941 GNRH1 Proteins 0.000 claims abstract description 8
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims abstract description 8
- 210000001015 abdomen Anatomy 0.000 claims abstract description 6
- 239000011259 mixed solution Substances 0.000 claims abstract description 6
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims abstract description 5
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims abstract description 5
- 229940015047 chorionic gonadotropin Drugs 0.000 claims abstract description 5
- 239000004575 stone Substances 0.000 claims description 42
- 102000002322 Egg Proteins Human genes 0.000 claims description 27
- 108010000912 Egg Proteins Proteins 0.000 claims description 27
- 210000004681 ovum Anatomy 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 230000032696 parturition Effects 0.000 claims description 16
- 230000008569 process Effects 0.000 claims description 14
- 230000012447 hatching Effects 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 230000037396 body weight Effects 0.000 claims description 4
- 230000001568 sexual effect Effects 0.000 claims description 4
- 244000144987 brood Species 0.000 claims description 3
- 230000008011 embryonic death Effects 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 235000008935 nutritious Nutrition 0.000 claims description 3
- 230000017448 oviposition Effects 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 6
- 238000011534 incubation Methods 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000004720 fertilization Effects 0.000 abstract description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 abstract description 3
- 239000001301 oxygen Substances 0.000 abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 239000000645 desinfectant Substances 0.000 abstract 1
- 241000132931 Batrachia Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000288007 Alectoris chukar Species 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 241000269436 Ranidae Species 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 244000046101 Sophora japonica Species 0.000 description 1
- 235000010586 Sophora japonica Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000003194 forelimb Anatomy 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a Quasipaa spinosa induced spawning and efficient breeding method and relates to the technical field of Quasipaa spinosa culture. The method comprises the steps of breeding frog selection; induced spawning, wherein the mixed solution containing chorionic gonadotropin, luteinizing hormone releasing hormone and normal saline is injected into the abdomen of each breeding frog, the use concentration of chorionic gonadotropin is 240-160 units/ml, the use concentration of luteinizing hormone releasing hormone is 7-9 micrograms/ml, 15-20 ml mixed solution is injected into each male frog, and 7-10 ml mixed solution is injected into each female frog; breeding; spawn treating, wherein a straw mat and spawns are transferred into a treating pool together, and the straw mat is beaten with a disinfectant stick to vibrate; incubation. According to the method, control over the cleanness, oxygen content and temperature of the Quasipaa spinosa breeding environment is reasonable, the fertilization rate of spawns subjected to induced spawning and spawn treating can reach over 95%, the hatchability of fertilized eggs is over 90%, effect time is shortened to 42 hours, spawn incubation time is shortened to 12.5 days, and the economic benefits of Quasipaa spinosa breeding are increased.
Description
Technical field
The present invention relates to stone frog cultural technique field, be specifically related to a kind of stone frog and hasten parturition efficient breeding method.
Background technology
The bamboo stone frog (Quasipaa spinosa) standard name Ranaspinosa David, have another name called stone clam, chukar, pheasant, stone freeze, flying fish, stone squama, stone frog, stone frog, the stone pit frog, stone unrest, wooden Chinese scholartree (Chuan Nan) etc., be a kind of animal of Amphibia Anura Ranidae.Stone frog tadpole body growth strip, tail is plump, and the colour of skin is dark yellow, and the black star point that distributes, look down " V " printed words decorative pattern of black at body and the back side of afterbody joining place, the muzzle of tadpole is flourishing, and very by force, stone frog body is large and sturdy for adsorption capacity.Become the long 10-13 centimetre of the general body of the frog, more than body weight 150-250 gram, large can reach more than 500 grams, stone frog pachylosis, skin of back becomes lead, and upper face length has much oil, the head of the stone frog is not only wide but also flat, and kiss is held into round, protrudes from lower jaw, distance between two nostrils and the distance between two almost equal, the stone frog of the same age is male to be greater than female, and the male frog forelimb after sexual maturity is very sturdy, chest has the corn in sour jujube shape, belly is faint yellow, female chest without quil, abdomen and smooth in white.The small tadpole of firm incubation of membrane, by incubating yellow capsule nutrition in 4 ~ 5 days, just starts to look for food after incubating Huang and being consumed.In the process that the artificial feeding stone frog breeds, the stone frog breeds the cleannes to environment, oxygen, the requirement of the conditions such as temperature is high especially, and the stone frog is different from common batrachia, the ovum that the stone frog produces is larger, almost mucous membrane is not had between ovum and ovum, comparatively dispersion residing for ovum, thus sperm can not be contacted fully with ovum, cause the spawning and fertilization rate of the stone frog in prior art low, intelligence reaches more than percent 80, the incubation rate of fertilized egg is also lower, cause raising stone frog economic benefit not high, and be all adopt the mode of hastening parturition of common batrachia for hastening parturition of the stone frog in prior art, the weak effect of hastening parturition, even uneven effect.
Summary of the invention
It is high that technical problem to be solved by this invention is to provide a kind of spawning and fertilization rate, and hatchability of fertile eggs is high, and the Effect time after hastening parturition and the short stone frog of brooding time are hastened parturition efficient breeding method.
Technical solution problem adopts following technical scheme,
A kind of stone frog is hastened parturition efficient breeding method, it is characterized in that: comprise the following steps,
Plant the selection of the frog
Select to raise through 10 ~ 12 months, reach sexual maturity, body weight requires at 250 ~ 400g, and without hindering without patient as kind of a frog, adopt natural propagation method, sex ration is 2: 1;
To hasten parturition process
The mixed solution of human chorionic gonadtropin, luteinizing hormone releasing hormone and physiological saline is used to carry out kind of a frog abdomen injection process, the working concentration of Chorionic Gonadotropin is 240 ~ 160 units/ml, the working concentration of luteinizing hormone releasing hormone is 7 ~ 9 μ g/ml, male frog injection volume is 15 ~ 20ml, and the injection volume of the female frog is 7 ~ 10ml;
Breeding
Stone is spread at the bottom of the pond of the foster stone frog, one deck straw mat is laid on stone top, the stone frog lays eggs from the first tenday period of a month in May, until terminate by the end of September, egg-laying period water temperature controls at 18 ~ 22 DEG C, and the sexually matured close frog oestrused can lie prone in mating season the tweeting sound sending on straw mat and seek a spouse, so as to attracting the other side, it is right to embrace in pairs after contact, and the male frog is lied prostrate in female frog back, and after the female frog lays eggs, namely the male frog ejaculates rapidly on ovum;
Ovum process
Ovum just under on straw mat after, straw mat is together transferred in treatment pond together with ovum, disinfect, water lev el control in treatment pond is at 20 ~ 30cm, straw mat is lain in a horizontal plane in pond, make the water in pond just flood straw mat, use the waddy of sterilization to beat straw mat and make it vibrate, notice that ovum place avoided by waddy;
Hatching
Pull out gently after when the morning 8, the ovum handled well is transferred in hatchery together with straw mat, hatchery area at 4 ~ 5m2, the dark 80 ~ 90cm in pond, water lev el control is at 70 ~ 80cm, putting ovum density is every square metre about 900 ~ 1100, a small amount of clear water will be had in hatching process to flow, and guarantee clean, water temperature controls between 15-30 DEG C, pH value is 6-8, and a pond is a brood of.
The frog's egg that described breeding is produced can not be hatched in native pond, is covered in case frog's egg falls and causes embryonic death at the bottom of pond by earth.
The tadpole that described hatching has just gone out is afraid of fierce vibration, and environment of hatching be kept quiet as far as possible, and keep bait abundance nutritious, water temperature controls 19 ~ 22 DEG C time.
The invention has the beneficial effects as follows: the stone frog of the present invention breeds the cleannes of environment, oxygen, temperature control rationally, through hastening parturition and the spawning and fertilization rate of ovum process up to more than 95%, the incubation rate of fertilized egg is also higher by more than 90%, shorten Effect time to 42H and egg hatching time by 12.5 days, be conducive to improving raising stone frog economic benefit.
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
A kind of stone frog is hastened parturition efficient breeding method, comprises the following steps,
Plant the selection of the frog
Select to raise through 10 ~ 12 months, reach sexual maturity, body weight requires at 250 ~ 400g, and without hindering without patient as kind of a frog, adopt natural propagation method, sex ration is 2: 1;
To hasten parturition process
The mixed solution of human chorionic gonadtropin, luteinizing hormone releasing hormone and physiological saline is used to carry out kind of a frog abdomen injection process, the working concentration of Chorionic Gonadotropin is 240 ~ 160 units/ml, the working concentration of luteinizing hormone releasing hormone is 7 ~ 9 μ g/ml, male frog injection volume is 15 ~ 20ml, and the injection volume of the female frog is 7 ~ 10ml;
Breeding
Stone is spread at the bottom of the pond of the foster stone frog, one deck straw mat is laid on stone top, the stone frog lays eggs from the first tenday period of a month in May, until terminate by the end of September, egg-laying period water temperature controls at 18 ~ 22 DEG C, and the sexually matured close frog oestrused can lie prone in mating season the tweeting sound sending on straw mat and seek a spouse, so as to attracting the other side, it is right to embrace in pairs after contact, and the male frog is lied prostrate in female frog back, and after the female frog lays eggs, namely the male frog ejaculates rapidly on ovum;
Ovum process
Ovum just under on straw mat after, straw mat is together transferred in treatment pond together with ovum, disinfect, water lev el control in treatment pond is at 20 ~ 30cm, straw mat is lain in a horizontal plane in pond, make the water in pond just flood straw mat, use the waddy of sterilization to beat straw mat and make it vibrate, notice that ovum place avoided by waddy;
Hatching
Pull out gently after when the morning 8, the ovum handled well is transferred in hatchery together with straw mat, hatchery area at 4 ~ 5m2, the dark 80 ~ 90cm in pond, water lev el control is at 70 ~ 80cm, putting ovum density is every square metre about 900 ~ 1100, a small amount of clear water will be had in hatching process to flow, and guarantee clean, water temperature controls between 15-30 DEG C, pH value is 6-8, and a pond is a brood of.
Breed the frog's egg produced to hatch in native pond, covered by earth at the bottom of pond in case frog's egg falls and cause embryonic death.
Hatch the tadpole just gone out and be afraid of fierce vibration, environment of hatching be kept as far as possible quiet, keep bait abundance nutritious, water temperature controls 19 ~ 22 DEG C time.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (3)
1. the stone frog is hastened parturition an efficient breeding method, it is characterized in that: comprise the following steps,
Plant the selection of the frog
Select to raise through 10 ~ 12 months, reach sexual maturity, body weight requires at 250 ~ 400g, and without hindering without patient as kind of a frog, adopt natural propagation method, sex ration is 2: 1;
To hasten parturition process
The mixed solution of human chorionic gonadtropin, luteinizing hormone releasing hormone and physiological saline is used to carry out kind of a frog abdomen injection process, the working concentration of Chorionic Gonadotropin is 240 ~ 160 units/ml, the working concentration of luteinizing hormone releasing hormone is 7 ~ 9 μ g/ml, male frog injection volume is 15 ~ 20ml, and the injection volume of the female frog is 7 ~ 10ml;
Breeding
Stone is spread at the bottom of the pond of the foster stone frog, one deck straw mat is laid on stone top, the stone frog lays eggs from the first tenday period of a month in May, until terminate by the end of September, egg-laying period water temperature controls at 18 ~ 22 DEG C, and the sexually matured close frog oestrused can lie prone in mating season the tweeting sound sending on straw mat and seek a spouse, so as to attracting the other side, it is right to embrace in pairs after contact, and the male frog is lied prostrate in female frog back, and after the female frog lays eggs, namely the male frog ejaculates rapidly on ovum;
Ovum process
Ovum just under on straw mat after, straw mat is together transferred in treatment pond together with ovum, disinfect, water lev el control in treatment pond is at 20 ~ 30cm, straw mat is lain in a horizontal plane in pond, make the water in pond just flood straw mat, use the waddy of sterilization to beat straw mat and make it vibrate, notice that ovum place avoided by waddy;
Hatching
Pull out gently after when the morning 8, transfer in hatchery by the ovum handled well together with straw mat, hatchery area is at 4 ~ 5m
2, the dark 80 ~ 90cm in pond, water lev el control is at 70 ~ 80cm, and putting ovum density is every square metre about 900 ~ 1100, and a small amount of clear water will be had in hatching process to flow, and guarantee clean, water temperature controls between 15-30 DEG C, and pH value is 6-8, and a pond is a brood of.
2. a kind of stone frog according to claim 1 is hastened parturition efficient breeding method, and it is characterized in that, the frog's egg that described breeding is produced can not be hatched in native pond, is covered in case frog's egg falls and causes embryonic death at the bottom of pond by earth.
3. a kind of stone frog according to claim 2 is hastened parturition efficient breeding method, it is characterized in that, the tadpole that described hatching has just gone out is afraid of fierce vibration, and environment of hatching be kept quiet as far as possible, and keep bait abundance nutritious, water temperature controls 19 ~ 22 DEG C time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410572372.1A CN104365540A (en) | 2014-10-23 | 2014-10-23 | Quasipaa spinosa induced spawning and efficient breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410572372.1A CN104365540A (en) | 2014-10-23 | 2014-10-23 | Quasipaa spinosa induced spawning and efficient breeding method |
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| CN104365540A true CN104365540A (en) | 2015-02-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410572372.1A Pending CN104365540A (en) | 2014-10-23 | 2014-10-23 | Quasipaa spinosa induced spawning and efficient breeding method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070040A (en) * | 2016-06-27 | 2016-11-09 | 益阳市丰运农业综合科技发展有限公司 | A kind of hatching method of Rana nigromaculata Rana ovum |
| CN109258567A (en) * | 2018-10-10 | 2019-01-25 | 安徽省农业科学院水产研究所 | A method of improving toad ovum rate of fertilization |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101461345A (en) * | 2008-12-22 | 2009-06-24 | 建德市洪村石蛙生态养殖场 | Intensive style method for hatching RanaspinosaDavid |
| CN101658157A (en) * | 2009-09-09 | 2010-03-03 | 吴诚根 | Rana spinosa eggmass collecting device and collecting method thereof |
| CN101904315A (en) * | 2010-07-05 | 2010-12-08 | 何明华 | Method for ecologically culturing Rana spinosa |
-
2014
- 2014-10-23 CN CN201410572372.1A patent/CN104365540A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101461345A (en) * | 2008-12-22 | 2009-06-24 | 建德市洪村石蛙生态养殖场 | Intensive style method for hatching RanaspinosaDavid |
| CN101658157A (en) * | 2009-09-09 | 2010-03-03 | 吴诚根 | Rana spinosa eggmass collecting device and collecting method thereof |
| CN101904315A (en) * | 2010-07-05 | 2010-12-08 | 何明华 | Method for ecologically culturing Rana spinosa |
Non-Patent Citations (3)
| Title |
|---|
| 仄凌: "棘胸蛙的繁殖培育管理", 《浙江林业》 * |
| 王建国等: "《致富霸王丛书 青蛙、石蛙养殖》", 30 September 2002 * |
| 陈三木: "棘胸蛙人工繁殖及苗种培育技术", 《北京水产》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070040A (en) * | 2016-06-27 | 2016-11-09 | 益阳市丰运农业综合科技发展有限公司 | A kind of hatching method of Rana nigromaculata Rana ovum |
| CN109258567A (en) * | 2018-10-10 | 2019-01-25 | 安徽省农业科学院水产研究所 | A method of improving toad ovum rate of fertilization |
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Application publication date: 20150225 |