CN104306961A - Method for eliminating latent dormant viruses or bacteria by utilizing excisionase - Google Patents
Method for eliminating latent dormant viruses or bacteria by utilizing excisionase Download PDFInfo
- Publication number
- CN104306961A CN104306961A CN201410542058.9A CN201410542058A CN104306961A CN 104306961 A CN104306961 A CN 104306961A CN 201410542058 A CN201410542058 A CN 201410542058A CN 104306961 A CN104306961 A CN 104306961A
- Authority
- CN
- China
- Prior art keywords
- virus
- nickase
- dormancy
- hide
- viruses
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000700605 Viruses Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 12
- 241000894006 Bacteria Species 0.000 title abstract description 6
- 108010055246 excisionase Proteins 0.000 title 1
- 239000003814 drug Substances 0.000 claims abstract description 3
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 17
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 17
- 230000005059 dormancy Effects 0.000 claims description 17
- 230000000844 anti-bacterial effect Effects 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 108091008146 restriction endonucleases Proteins 0.000 claims description 7
- 230000032258 transport Effects 0.000 claims description 6
- 238000011069 regeneration method Methods 0.000 claims description 5
- 230000003612 virological effect Effects 0.000 claims description 5
- 102100031780 Endonuclease Human genes 0.000 claims description 4
- 108010042407 Endonucleases Proteins 0.000 claims description 4
- 230000009849 deactivation Effects 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 3
- 101710163270 Nuclease Proteins 0.000 claims description 2
- 238000001784 detoxification Methods 0.000 claims description 2
- 230000007159 enucleation Effects 0.000 claims description 2
- 230000035876 healing Effects 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 239000003419 rna directed dna polymerase inhibitor Substances 0.000 claims description 2
- 230000009466 transformation Effects 0.000 claims description 2
- 208000030507 AIDS Diseases 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000002103 transcriptional effect Effects 0.000 abstract 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Current known method for treating bacteria and viruses is only capable of cleaning active viruses, such as AIDS and the like, but cannot eliminate latent viruses, so that bacteria or viruses cannot be cured. According to the method, a collected latent dormant virus or bacterium is sequenced, incision enzyme designing is completed by calculation and location of recognition sequence of the incision enzyme, and active viruses can be eliminated by combining reverse transcriptional inhibitor or other medicines for killing viruses or bacteria. Therefore, all viruses in a body can be completely eliminated, and the aim of curing can be achieved.
Description
Technical field
The present invention relates to one utilizes nickase to cut transcriptase and Retroviral method.
Technical background
Current known treatment is bacillary and the viral methods activity of can only removing is viral, can not remove dormant bacteria or virus, as removed movable virus before the viraleses such as AIDS, can not remove latent virus, so can not cure.
Summary of the invention
The object of the invention is; Utilize nickase remove hide dormancy virus or bacterial method.
The scheme that its technical problem of solution of the present invention adopts is; To hide dormancy virus or antibacterial order-checking through collection, rear calculating and location endonuclease recognition sequence carry out complete design restriction endonuclease. restriction endonuclease by instrument in transporting body as cut the some position of hide dormancy virus or antibacterial in deactivation cell entry body. utilize the virus of transformation detoxification to transport for carrier transports instrument the nickase designed. cut point position peplos and hide dormancy virus or bacterial sequences. design different nickase according to different virus. make patients ' recovery by cell self-regeneration again.Entering body interior to dormancy virus or the cutting of antibacterial point position of hiding. nickase includes the restriction endonuclease of cutting function, nuclease etc. and be transported to focus and can take local injection mode and viral enucleation itself will be treated to transform means of transport arrival focus as.Kill the virus in conjunction with reverse transcriptase inhibitor or other again or the virus of medicine removing activity of antibacterial.Just can all virus in purged body completely, reach healing object.
Benefit of the present invention is; Removing hide dormancy virus or antibacterial, side effect is little, targeted elimination.
Below in conjunction with accompanying drawing, the invention will be further described
Fig. 1 be utilize nickase remove hide dormancy virus or bacterial method
In figure, the interior instrument 5 deactivation virus 6 of 1 restriction endonuclease 4 transporting body that collection dormancy is viral or antibacterial order-checking 2 calculates and location endonuclease recognition sequence 3 designs enters in body and cuts 7 cell self-regeneration 8 rehabilitations to promoter and other some positions
In FIG
Through gathering dormancy virus or antibacterial order-checking (1), rear calculating and the good nickase (3) of location endonuclease recognition sequence (2) complete design.The nickase (3) designed cuts (6) such as deactivation virus (5) enters in body to promoter and other some positions by instrument (4) interior in transporting body.Patient (7) rehabilitation (8) is made again by cell self-regeneration.
Claims (4)
1. utilize nickase remove hide dormancy virus or a bacterial method, it is characterized in that; Through collection hide dormancy virus or antibacterial order-checking, rear calculating and location endonuclease recognition sequence carry out complete design restriction endonuclease.Restriction endonuclease by instrument in transporting body as cut the some position of hide dormancy virus or antibacterial in deactivation cell entry body.
2. the one according to claims (1) utilize nickase remove hide dormancy virus or bacterial method, it is characterized in that; The virus of transformation detoxification is utilized to transport for carrier transports instrument the nickase designed. cut point position peplos and hide dormancy virus or bacterial sequences.Different nickase is designed according to different virus. make patients ' recovery by cell self-regeneration again.Be transported to focus can take local injection mode and viral enucleation itself will be treated to transform means of transport arrival focus as.Kill the virus in conjunction with reverse transcriptase inhibitor or other again or the virus of medicine removing activity of antibacterial.Just can all virus in purged body completely, reach healing object.
3. the one according to claims (1) utilize nickase remove hide dormancy virus or bacterial method, it is characterized in that; Cut point position peplos and bacterial sequences.Different nickase is designed according to different virus.Nickase includes the restriction endonuclease of cutting function, nuclease etc.
4. the one according to claims (1) utilize nickase remove hide dormancy virus or bacterial method, it is characterized in that; Patients ' recovery is made again by cell self-regeneration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410542058.9A CN104306961A (en) | 2014-10-05 | 2014-10-05 | Method for eliminating latent dormant viruses or bacteria by utilizing excisionase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410542058.9A CN104306961A (en) | 2014-10-05 | 2014-10-05 | Method for eliminating latent dormant viruses or bacteria by utilizing excisionase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104306961A true CN104306961A (en) | 2015-01-28 |
Family
ID=52362370
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410542058.9A Pending CN104306961A (en) | 2014-10-05 | 2014-10-05 | Method for eliminating latent dormant viruses or bacteria by utilizing excisionase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104306961A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4024530A1 (en) * | 1990-08-02 | 1992-02-06 | Klinger & Co Dr | Control of viral disease using bovine pancreatic nuclease(s) - for treatment of e.g. rabies, herpes, Epstein-Barr virus and cow-pox |
| WO1993020207A1 (en) * | 1992-04-02 | 1993-10-14 | The United States Of America, As Represented By The Secretary Of Health And Human Services | Use of restriction endonucleases against viruses, including hiv |
| WO1996022368A1 (en) * | 1995-01-18 | 1996-07-25 | Gene Shears Pty. Ltd. | Ribozymes targeting the retroviral packaging sequence expression constructs and recombinant retroviruses containing such constructs |
| EP0979655A1 (en) * | 1998-08-01 | 2000-02-16 | Boehringer Mannheim Gmbh | Means for treatment of infections caused by RNA-Virusses such as HIV |
| WO2001094391A2 (en) * | 2000-06-08 | 2001-12-13 | Incyte Genomics, Inc. | Intracellular signaling proteins |
| CN1364898A (en) * | 2002-02-05 | 2002-08-21 | 中国人民解放军第四军医大学 | Process for preparing target ribonuclease for curing hepatitis B virus infection |
| CN1490050A (en) * | 2002-10-17 | 2004-04-21 | 中国医学科学院医学生物学研究所 | Hepatitis A inactivated vaccine |
| CA2549663A1 (en) * | 2005-06-06 | 2006-12-06 | University Of Northern British Columbia | Endoribonuclease and uses thereof |
| CN101250510A (en) * | 2008-03-19 | 2008-08-27 | 广东药学院 | Construction method and application of hepatitis C virus specific ribozyme M1GS-hcv/C20 |
-
2014
- 2014-10-05 CN CN201410542058.9A patent/CN104306961A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4024530A1 (en) * | 1990-08-02 | 1992-02-06 | Klinger & Co Dr | Control of viral disease using bovine pancreatic nuclease(s) - for treatment of e.g. rabies, herpes, Epstein-Barr virus and cow-pox |
| WO1993020207A1 (en) * | 1992-04-02 | 1993-10-14 | The United States Of America, As Represented By The Secretary Of Health And Human Services | Use of restriction endonucleases against viruses, including hiv |
| US5523232A (en) * | 1992-04-02 | 1996-06-04 | The United States Of America As Represented By The Department Of Health And Human Services | Use of restriction endonucleases against viruses, including HIV |
| WO1996022368A1 (en) * | 1995-01-18 | 1996-07-25 | Gene Shears Pty. Ltd. | Ribozymes targeting the retroviral packaging sequence expression constructs and recombinant retroviruses containing such constructs |
| EP0979655A1 (en) * | 1998-08-01 | 2000-02-16 | Boehringer Mannheim Gmbh | Means for treatment of infections caused by RNA-Virusses such as HIV |
| WO2001094391A2 (en) * | 2000-06-08 | 2001-12-13 | Incyte Genomics, Inc. | Intracellular signaling proteins |
| CN1364898A (en) * | 2002-02-05 | 2002-08-21 | 中国人民解放军第四军医大学 | Process for preparing target ribonuclease for curing hepatitis B virus infection |
| CN1490050A (en) * | 2002-10-17 | 2004-04-21 | 中国医学科学院医学生物学研究所 | Hepatitis A inactivated vaccine |
| CA2549663A1 (en) * | 2005-06-06 | 2006-12-06 | University Of Northern British Columbia | Endoribonuclease and uses thereof |
| CN101250510A (en) * | 2008-03-19 | 2008-08-27 | 广东药学院 | Construction method and application of hepatitis C virus specific ribozyme M1GS-hcv/C20 |
Non-Patent Citations (4)
| Title |
|---|
| JEROME MULHBACHER等: "Therapeutic applications of ribozymes and riboswitches", 《CURRENT OPINION IN PHARMACOLOGY》 * |
| SANJEEV SINGWI等: "POTENTIAL NUCLEASE-BASED STRATEGIES FOR HIV GENE THERAPY", 《FRONTIERS IN BIOSCIENCE》 * |
| 付勇等: "核糖核酸酶的抗肿瘤研究进展", 《国外医学·生理、病理科学与临床分册》 * |
| 李红等: "脱氧核酶在病毒学中的研究和应用", 《中国兽药杂志》 * |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150128 |
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| WD01 | Invention patent application deemed withdrawn after publication |