CN104266979B - A kind of element, label and its discrimination method using the element for being used to recognize the mark containing bacteria rhodopsin or its variant - Google Patents

Abstract

The invention provides an element for identifying a marker containing bacteriorhodopsin or a variant thereof, comprising a filter assembly, the filter assembly selectively transmits visible light with a wavelength of 360-450nm, and the peak transmittance The pass rate is greater than 5%; a kind of anti-counterfeiting marker and its kit are also provided, comprising the marker containing bacteriorhodopsin or its variants arranged on the carrier and the present invention for identifying bacteriorhodopsin or its variants An element of a body mark, the element may be provided on the carrier, or may exist independently. The identification method of the anti-counterfeiting mark of the present invention is to place the element above the mark containing bacteriorhodopsin or its variants, irradiate the element with visible light, and let the visible light pass through the element and project on the mark, The authenticity of said item can be identified by the purple pattern displayed on the mark.

Description

An element for identifying a marker containing bacteriorhodopsin or a variant thereof, using Marker using the element and identification method thereof

technical field

The present invention relates to a method for identifying the authenticity of articles, in particular to an element for identifying a mark containing bacteriorhodopsin or a variant thereof, an anti-counterfeit marker or an anti-counterfeit marker kit using the element, and identification of an anti-counterfeit mark method.

Background technique

Photochromism refers to the phenomenon that the color of a substance changes reversibly under the irradiation of light of appropriate wavelength. This color change can be naturally restored by removing the excitation, irradiating it with another light of a different wavelength, or increasing the temperature. Among them, bacteriorhodopsin is a photosensitive biomaterial with photochromic properties.

Bacteriorhodopsin is a two-dimensional crystal naturally formed on the cell membrane of wild halophilic bacteria—the only protein component in the purple membrane. It has excellent stability in vitro and does not lose its activity for a long time. The bacteriorhodopsin molecule is composed of 248 amino acid residues, and its molecular weight is 26,000 Daltons. The protein molecule is folded in space to form 7 transmembrane helical columns. The N-terminus is located outside the cell membrane, and the C-terminus is located on the inside of the cell membrane. The helical columns are basically vertical In the cell membrane, each BR protein binds a retinal chromophore on the 216th amino acid residue inside the cell membrane. The above structural properties make bacteriorhodopsin and its variants rapidly undergo isomerization changes of retinal molecules after absorbing photons, and correspondingly transform into intermediate states with different absorption peaks, so that they can be produced under different intensity (light and dark) environments of visible light. Color change visible to the naked eye.

Utilizing the photochromic properties of bacteriorhodopsin and its variants, Chinese patent document CN1347371A discloses a method of applying photochromic ink to articles to protect the authenticity of articles by adding at least one A bacteriorhodopsin variant that is a photochromic moiety that undergoes a visually detectable and reversible state change, especially a color change, when irradiated with wavelengths in the visible range, for use as a low-level authenticity test. security features. However, in practical application, people may test it outdoors or in a dimly lit meeting room. The color of rhodopsin or its variants is different, which reduces the recognition rate and causes misjudgment and misjudgment of the authenticity of the item. Just because of this, it is used as a low-level security feature in the method for protecting the authenticity of the article recorded in the above-mentioned patent documents, so that in addition to the low-level security feature, it also has a high-level security feature that can only be detected by instrumental analysis and cannot be detected visually. Security features, due to the misjudgment and misjudgment of the low-level security features in the methods disclosed in the above documents, the significance of its existence is also greatly reduced. In addition, the color change of rhodopsin requires a certain ink layer thickness, and its saturation High, the discoloration effect will be more obvious after the light source is excited, but the current printing process, the pattern is not too thick, which leads to a small amount of ink, so that the rhodopsin-containing mark printed on the article Its rhodopsin content is too little, which reduces the saturation of its color change, further reducing the recognition rate of the marker.

In summary, in order to improve the recognition rate of markers containing bacteriorhodopsin or its variants, it is necessary to increase the intensity of light. However, due to the limitation of the existing printing process, even if the light intensity meets the requirements, due to the content of bacteriorhodopsin in the marker If it is too small, the recognition rate of 100% cannot be achieved, resulting in misjudgment and misjudgment of the authenticity of the item. Therefore, how to improve the recognition rate of marks containing bacteriorhodopsin or its variants under the existing printing process becomes A problem that needs to be solved at present.

Contents of the invention

In order to solve the problem that the discoloration of rhodopsin or its variants in the prior art responds to the ambient light at all times, the ambient light is not fixed, and the colors of rhodopsin are different, resulting in misjudgment and misjudgment of the authenticity of the item. problem, and the rhodopsin-containing mark printed by the printing process in the prior art reduces its discoloration saturation due to too little content of rhodopsin, thereby reducing the problem of the recognition rate of the mark, and then provides a method to identify the authenticity of the article The method, and further provides a component that uses the method to detect the authenticity of the item.

For this reason, the technical scheme that the present invention takes is,

The present invention provides an element for identifying a marker containing bacteriorhodopsin or a variant thereof, the element includes a filter assembly, the filter assembly selectively transmits visible light with a wavelength of 360-450nm, and the The peak transmittance is greater than 5%.

Preferably, in the above-mentioned element for identifying a marker containing bacteriorhodopsin or a variant thereof, the peak transmittance is greater than 20%.

Preferably, in the above-mentioned element for identifying a marker containing bacteriorhodopsin or a variant thereof, the filter component is a color temperature paper, a filter or an ink coating.

Preferably, the above-mentioned element for identifying a marker containing bacteriorhodopsin or a variant thereof further includes a light-transmitting component, and the light-transmitting component is arranged on the light-transmitting component.

In the above-mentioned element for identifying the marker containing bacteriorhodopsin or its variants on the article, the setting is embedding.

The present invention also provides an anti-counterfeiting marker, comprising a marker containing bacteriorhodopsin or its variants arranged on a carrier, and any one of the above-mentioned markers for identifying bacteriorhodopsin-containing substances arranged on the carrier or a tagged element of a variant thereof.

Preferably, in the above-mentioned anti-counterfeiting marker, the mark containing bacteriorhodopsin or its variants is made of ink containing bacteriorhodopsin or its variants, and the added amount of bacteriorhodopsin or its variants is The mass is 5-30wt% of the total mass of the ink.

Preferably, in the aforementioned anti-counterfeiting marker, the bacteriorhodopsin variant is D96N or D85T.

Preferably, in the above-mentioned anti-counterfeit marker, the carrier is made of paper and/or polymer material.

The present invention also provides a set of anti-counterfeiting markers, comprising a marker containing bacteriorhodopsin or a variant thereof arranged on a carrier, characterized in that it also includes any of the above-mentioned independent markers for identifying bacteriorhodopsin-containing or a tagged element of a variant thereof.

Preferably, in the above-mentioned anti-counterfeiting marker kit, the mark containing bacteriorhodopsin or its variants is made of ink containing bacteriorhodopsin or its variants, and the added amount of bacteriorhodopsin or its variants is The mass is 5-30wt% of the total mass of the ink.

Preferably, in the aforementioned anti-counterfeiting marker kit, the bacteriorhodopsin variant is D96N or D85T.

Preferably, in the aforementioned anti-counterfeit marker kit, the carrier is made of paper and/or polymer materials.

The present invention also provides a method for identifying an anti-counterfeit mark, wherein any of the above-mentioned elements for identifying a mark containing bacteriorhodopsin or a variant thereof is placed above the mark containing bacteriorhodopsin or a variant thereof, Visible light is used to irradiate the element, so that the visible light passes through the element and is projected on the mark, and the authenticity of the article is identified according to the pattern displayed on the mark, when the shape of the pattern is consistent with the shape of the filter assembly true when , otherwise false.

Compared with the prior art, the present invention has the following advantages,

(1) When visible light is irradiated on the label containing bacteriorhodopsin or its variants, reversible photosensitization isomerization occurs after the rhodopsin absorbs photons. This light cycle reaction contains a series of intermediates, among which The transformation of intermediates is accompanied by corresponding changes in material structure, and intermediates of different structures have different effects on the color development of rhodopsin; the applicant creatively found that when visible light with a wavelength of 360-450nm is selectively transmitted, and the When the above-mentioned peak transmittance is greater than 5%, when the label containing bacteriorhodopsin or its variants is irradiated, it is more likely to change to the purple B state, thus presenting a higher degree of recognition.

(2) The element for identifying markers containing bacteriorhodopsin or its variants according to the present invention further includes a light-transmitting component, and the filter component is embedded on the light-transmitting component, so that when visible light is emitted by the When the element is filtered and irradiated onto the mark, there is a difference in wavelength between the light transmitted by the light-transmitting component and the light transmitted by the filter component, which further increases the difference between the colored rhodopsin pattern and the surrounding color, making the display The color is more obvious, increasing the degree of recognition.

(3) In the anti-counterfeiting marker of the present invention or its kit, the addition amount of the bacteriorhodopsin or its variants is 5-30wt% of the total mass of the ink in terms of mass, and is used in conjunction with the present invention. After the element filters the visible light and irradiates the mark, the effect of other substances in the ink on the rhodopsin discoloration is avoided, so that the printed mark with a low rhodopsin content has a better color rendering effect.

(4) The method for protecting the authenticity of the article according to the present invention has a high recognition rate, reduces misjudgment or misjudgment of the real article, and has higher anti-counterfeiting performance, and the cooperative use of the identification mark and the detection element increases the difficulty of article imitation , reducing the appearance of imitations.

Description of drawings

In order to make the content of the present invention more easily understood, the present invention will be described in further detail below according to specific embodiments of the present invention in conjunction with the accompanying drawings, wherein

Figure 1 is an element of the present invention for identifying markers containing bacteriorhodopsin or variants thereof;

Fig. 2 is another element of the present invention for identifying markers containing bacteriorhodopsin or its variants;

Fig. 3 is another element of the present invention for identifying markers containing bacteriorhodopsin or its variants;

Figure 4 is a schematic diagram of an invoice using the elements described in Example 1;

Fig. 5 is a schematic diagram of a document using the components described in Embodiment 3 and the identification method of the document.

where the reference numerals denote,

1-filter component; 2-light-transmitting component; 3-mark; 4-element.

detailed description

Embodiments 1-3 are embodiments of the elements used for identifying markers containing bacteriorhodopsin or variants thereof according to the present invention.

Example 1

As shown in Figure 1, it is an element used to identify markers containing bacteriorhodopsin or its variants, the element only consists of a filter assembly, and the filter assembly selectively transmits visible light with a wavelength of 360-450nm , and the peak transmittance is greater than 5%. In this embodiment, the filter assembly is a square filter.

In other embodiments, the filter assembly can also be color temperature paper that meets the above light transmission requirements, and the filter assembly can be formed of color temperature paper or filters in various shapes.

As a preferred embodiment, the filter assembly selectively transmits visible light with a wavelength of 360-450 nm, and the peak transmittance is greater than 20%.

Example 2

As shown in Figure 2, it is yet another element used to identify markers containing bacteriorhodopsin or its variants, the element includes a filter assembly 1, and the filter assembly selectively transmits light having a wavelength of 360-450nm. Visible light, and the peak transmittance is greater than 5%. In this embodiment, the filter component is color temperature paper, and the element further includes a light-transmitting component 2 , and the filter component is embedded on the light-transmitting component. In this embodiment, the filter component 1 is a "¥"-shaped pattern formed by color temperature paper, which is embedded in a circular light-transmitting component. In this embodiment, the light-transmitting component is a piece of paper.

In other embodiments, the filter assembly 1 can also be a filter that meets the above light transmission requirements, and the filter assembly 1 can be various patterns formed by color temperature paper or filters, and the light transmission assembly It can also be a material formed of high molecular polymer, such as PET material.

As a preferred embodiment, the filter assembly selectively transmits visible light with a wavelength of 360-450 nm, and the peak transmittance is greater than 20%.

Example 3

Another element for identifying a marker containing bacteriorhodopsin or its variants as shown in Figure 3, comprising a circular light-transmitting component 2 and a filter component 1 coated on the light-transmitting component 2, The filter assembly 1 is an ink coating printed with Marapol PY screen printing ink from Germany. The ink coating selectively transmits visible light with a wavelength of 360-450nm, and the peak transmittance is greater than 5%. In this embodiment, the ink coating (colored part) cooperates with the uncoated area (uncolored area) of the light-transmitting component 2 to form the pattern of a football. In other embodiments, the ink coating can also be other The shape of the light-transmitting component 2 is used to form other patterns, and the light-transmitting component 2 is made of paper, plastic or PET material. In this embodiment, the light-transmitting component is a PET sheet.

As a preferred embodiment, the ink region printed with the ink forms a filter assembly, which selectively transmits visible light with a wavelength of 360-450 nm, and the peak value of the transmittance is greater than 20%.

The element for identifying the mark containing bacteriorhodopsin or its variants on the above embodiment can be directly arranged on the marked item with bacteriorhodopsin or its variants, or can exist independently. The following examples are application examples of the elements of the present invention for identifying markers containing bacteriorhodopsin or variants thereof.

Example 4

As shown in Figure 4, it is an invoice with a mark 3, which is a square mark formed by applying ink containing bacteriorhodopsin variant D96N to the invoice body, wherein the amount of D96N added is the mass of the ink 5wt%; the invoice is also provided with an element 4 for identifying the mark, and the element 4 is the element 4 for identifying the mark containing bacteriorhodopsin or its variants on the article according to the present invention, The element described in this embodiment adopts the element 4 described in Embodiment 1. When detecting the authenticity of the invoice, the invoice is folded so that the element is placed above the mark 3, the element 4 is irradiated with visible light, and the visible light is projected on the mark 3 through the element 4 , when a purple square is displayed on mark 3, it indicates that the invoice is true, otherwise it is false.

Example 5

A document with a mark 3, the mark is a circular mark formed by applying ink containing bacteriorhodopsin variant D85T to the document body, wherein the added amount of D85T is 30wt% of the mass of the ink; The document is also provided with an element 4 for identifying the mark, and the element 4 is the element 4 for identifying the mark containing bacteriorhodopsin or its variants on the article according to the present invention. Said element adopts the element 4 described in embodiment 3 (as shown in FIG. 5 ). When detecting the authenticity of the document, the document is folded so that the element is placed above the mark 3, and the element 4 is irradiated with visible light, so that the visible light passes through the element 4 and is projected on the mark 3 , when the purple football pattern is displayed on mark 3, it indicates that the invoice is true, otherwise it is false.

The authenticity of the articles containing the markers of bacteriorhodopsin or its variants in the above-mentioned embodiment 4 or 5 can also directly adopt the independently existing markers of the present invention for identifying the markers containing bacteriorhodopsin or its variants Components for detection, that is, for items that need to be protected for authenticity, it can set a mark containing bacteriorhodopsin or its variants on the item, and then use the independently existing method for identifying items containing bacteriorhodopsin. The marked element of rhodopsin or its variants is detected, or as in embodiment 4 or 5, the element and the mark are arranged on the article at the same time, and the element of the article itself is used to detect the mark to determine the authenticity of the article . Wherein, for the element for identifying the marker containing bacteriorhodopsin or its variants according to the present invention provided on the article, the light-transmitting component used may be the same as or different from the material of the article itself, and the article may be Any item whose authenticity needs to be protected, such as merchandise, collectibles, notes, contracts or even currency, etc.

Evaluation example

Select 400 copies of gray paper of the same material, and use the existing screen printing process to print a "¥"-shaped mark on the same position on each page. The mark is printed with ink containing D96N to form a comparison; select the same Another 400 copies of gray paper were made, and a circular mark was printed on the same position of each page using the existing screen printing process. The mark was printed with the same ink containing D96N to form a sample example.

The environment for paper identification is outdoors with natural light and a closed room with the same space size with 25W, 50W, and 100W incandescent lamps respectively installed.

For the identification method of the comparison example, two experienced staff members were selected to visually identify the gray paper in the comparison example in the above four different environments, and the paper that can recognize the "￥" shaped mark was judged to be true. The paper on which the "¥"-shaped mark cannot be recognized was judged as false. The results of two workers were averaged.

Sample identification method, the above-mentioned two experienced staff, in the above-mentioned four different environments, respectively identify the gray paper in the sample example, and in the identification process, use the method used to identify the content of the article in Embodiment 2 of the present invention Identify the marked element of bacteriorhodopsin or its variants, that is, set the element above the circular mark, wherein the mark paper of "¥" shape can be recognized as true, and the mark of "¥" shape cannot be recognized The paper is judged as false. The results of two workers were averaged.

The error rate calculation method in the above recognition method is as follows, and the results are shown in Table 1

Error rate = (Number marked as false ÷ 400) x 100%.

Table 1

As can be seen from the data in Table 1, when the existing identification marks are observed with the naked eye, the color rendering of the pattern is greatly affected by the environment, which affects people's judgment, especially for more complex patterns, due to the existing printing The amount of ink used in the process is small. For complex patterns, the pattern deviation caused by slightly unsaturated color development or uneven ink caused by printing will affect people's mistakes in judging the authenticity of the item. In this application, however, the above-mentioned problems are avoided by adopting the components described in the present invention, and errors caused by people's recognition are reduced.

Apparently, the above-mentioned embodiments are only examples for clear description, rather than limiting the implementation. For those of ordinary skill in the art, other changes or changes in different forms can be made on the basis of the above description. It is not necessary and impossible to exhaustively list all the implementation manners here. And the obvious changes or changes derived therefrom are still within the scope of protection of the present invention.

Claims (9)

1. a kind of anti-fake marker, includes the mark containing bacteria rhodopsin or its variant being arranged on carrier, its feature It is, also comprising the element for being used to recognize the mark containing bacteria rhodopsin or its variant set on the carrier, institute Stating the element for recognizing the mark containing bacteria rhodopsin or its variant includes filtering assembly, the filtering assembly selectivity Through wavelength be 360-450nm visible ray, and peak transmittance be more than 5%, the filtering assembly be colour temperature paper, filter plate or Ink coating；Also include light-transmitting component, the filtering assembly is arranged on light-transmitting component.

2. anti-fake marker according to claim 1, it is characterised in that described containing bacteria rhodopsin or its variant Mark is made up of the ink containing bacteria rhodopsin or its variant, and the addition of bacteria rhodopsin or its variant is in mass For the 5-30wt% of the ink gross mass.

3. anti-fake marker according to claim 2, it is characterised in that the bacteria rhodopsin variant be D96N or D85T。

4. according to any described anti-fake markers of claim 1-3, it is characterised in that the carrier is by paper and/or macromolecule Material is made.

5. a kind of anti-fake marker external member, comprising the mark containing bacteria rhodopsin or its variant being arranged on carrier, its It is characterised by, it is described to be used to know also comprising the independent element for being used to recognize the mark containing bacteria rhodopsin or its variant The element of mark not containing bacteria rhodopsin or its variant includes filtering assembly, and the filtering assembly selectively passes through wavelength For 360-450nm visible ray, and peak transmittance is more than 5%, and the filtering assembly is that colour temperature paper, filter plate or ink are applied Layer；Also include light-transmitting component, the filtering assembly is arranged on light-transmitting component.

6. anti-fake marker external member according to claim 5, it is characterised in that described containing bacteria rhodopsin or its change The mark of body is made up of the ink containing bacteria rhodopsin or its variant, and the addition of bacteria rhodopsin or its variant is with matter Amount is calculated as the 5-30wt% of the ink gross mass.

7. anti-fake marker external member according to claim 6, it is characterised in that the bacteria rhodopsin variant is D96N Or D85T.

8. according to any described anti-fake marker external members of claim 5-7, it is characterised in that the carrier is by paper and/or height Molecular material is made.

9. a kind of discrimination method of anti-fake mark, it is characterised in that by for recognizing containing bacteria rhodopsin or its variant The element of mark is placed on the top of the mark containing bacteria rhodopsin or its variant, using element described in radiation of visible light, Element projection described in visible light-transmissive is set on this mark, according to the true and false of the pattern recognition article shown on mark, to work as institute State pattern shape it is consistent with the shape of filtering assembly when be true, be otherwise false；

The element for being used to recognize the mark containing bacteria rhodopsin or its variant includes filtering assembly, the filtering assembly Selectivity is through the visible ray that wavelength is 360-450nm, and peak transmittance is more than 5%, and the filtering assembly is colour temperature paper, filter Wave plate or ink coating；Also include light-transmitting component, the filtering assembly is arranged on light-transmitting component.