CN104232716A - Soybean antihypertensive peptide and preparation method thereof - Google Patents
Soybean antihypertensive peptide and preparation method thereof Download PDFInfo
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- CN104232716A CN104232716A CN201410423272.2A CN201410423272A CN104232716A CN 104232716 A CN104232716 A CN 104232716A CN 201410423272 A CN201410423272 A CN 201410423272A CN 104232716 A CN104232716 A CN 104232716A
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- soybean
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 35
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 28
- 244000068988 Glycine max Species 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 230000003276 anti-hypertensive effect Effects 0.000 title abstract description 5
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 230000007062 hydrolysis Effects 0.000 claims abstract description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 6
- 108090000790 Enzymes Proteins 0.000 claims abstract description 6
- 239000003513 alkali Substances 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 5
- 238000005507 spraying Methods 0.000 claims abstract description 5
- 102000035195 Peptidases Human genes 0.000 claims abstract description 3
- 108091005804 Peptidases Proteins 0.000 claims abstract description 3
- 238000010438 heat treatment Methods 0.000 claims abstract description 3
- 230000036772 blood pressure Effects 0.000 claims description 31
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 108010073771 Soybean Proteins Proteins 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 7
- 235000019710 soybean protein Nutrition 0.000 claims description 7
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- 229940088598 enzyme Drugs 0.000 claims description 5
- 230000009849 deactivation Effects 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 2
- 230000017854 proteolysis Effects 0.000 claims description 2
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000000047 product Substances 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract 2
- 238000001035 drying Methods 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 206010020772 Hypertension Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 102000011632 Caseins Human genes 0.000 description 3
- 108010076119 Caseins Proteins 0.000 description 3
- 101000984728 Chiropsoides quadrigatus Angiotensin-converting enzyme inhibitory peptide Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000004531 blood pressure lowering effect Effects 0.000 description 3
- 239000005018 casein Substances 0.000 description 3
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 3
- 235000021240 caseins Nutrition 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 229940079593 drug Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001077 hypotensive effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- HDACQVRGBOVJII-JBDAPHQKSA-N ramipril Chemical compound C([C@@H](C(=O)OCC)N[C@@H](C)C(=O)N1[C@@H](C[C@@H]2CCC[C@@H]21)C(O)=O)CC1=CC=CC=C1 HDACQVRGBOVJII-JBDAPHQKSA-N 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 239000005541 ACE inhibitor Substances 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 208000020446 Cardiac disease Diseases 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 108010028690 Fish Proteins Proteins 0.000 description 1
- 208000007177 Left Ventricular Hypertrophy Diseases 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 206010042434 Sudden death Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229940077927 altace Drugs 0.000 description 1
- 229940044094 angiotensin-converting-enzyme inhibitor Drugs 0.000 description 1
- 230000004872 arterial blood pressure Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 229940125532 enzyme inhibitor Drugs 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000001631 hypertensive effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 229960003401 ramipril Drugs 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a preparation method of a soybean peptide and belongs to the field of food functional agents and preparation methods thereof. The preparation method of the soybean antihypertensive peptide comprises the following steps: (1) mixing marketed soybean isolate proteins with aqueous alkali, adding a proteolytic enzyme to hydrolyze, and after hydrolysis, heating to inactivate enzyme; (2) sequentially centrifuging, filtering and ultra-filtering the hydrolysate to obtain a filtrate; and (3) decompressing, concentrating, spraying and drying the filtrate to prepare yellowish and offwhite powder which is the soybean antihypertensive peptide. The soybean antihypertensive peptide provided by the invention has the characteristics of simple process, strong operability and high yield and product quality, and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of preparation method of soybean peptides, belong to food function agent and preparation method thereof field.
Background technology
Hypertension is the major cause causing the serious disease such as apoplexy, renal failure, left ventricular hypertrophy, heart disorder, arteriosclerosis, coronary heart disease, sudden death, and it is the heart, brain, kidney organ " invisible killer ".According to the statistical information display of national hygiene department, the existing hypertensive patient of China has reached 1.3 hundred million people, and with the speed increase of annual 3000000 new patients.Prevention and therapy hypertension has become the national instant task of China.
But hypertension is a puzzlement people's difficult problem, up to now, there is no good methods for the treatment of always.Angiotensin-convertion enzyme inhibitor (ACEI) is good Altace Ramipril, in clinical treatment, also achieve certain effect.But, the past be mostly synthesize ACE inhibitor.Hyperpietic's long-term taking synthesis hypotensor can produce the side effect of various discomfort, and present medical worker and patient tend to luxuriant and rich with fragrance synthetic drugs therapy.In recent years large quantity research shows, in natural food albumen, also separable go out ace inhibitory peptide.Japan NAOYUKI etc. extracts blood pressure lowering peptide from Hydrolysates of Casein, and external and animal experiment shows that casein antihypertensive peptides has strong hypotensive activity; Japan has been proposed fish-protein beverage for reducing blood pressure.Although the blood pressure lowering effect of protein peptide is strong not as good as synthetic drugs, these ace inhibitory peptides without impact, are the comparatively ideal hypotensor of a class on normal arterial pressure.In vegetable-protein and animal proteinum all separable go out ace inhibitory peptide, as soybean peptides, casein peptide, flesh of fish peptide etc.
Therefore, study a kind of blood pressure lowering peptide prepared from soybean protein and there is obvious economic and social profit.
Summary of the invention
It is simple, workable that the present invention aims to provide a kind of technique, is applicable to the preparation method of suitability for industrialized production and the rate of recovery, soybean blood pressure lowering peptide that quality product is high.
The preparation method of soybean blood pressure lowering peptide of the present invention, comprises the following steps:
(1) mixed with alkali aqueous solution by commercially available soybean protein isolate, add proteolytic enzymes hydrolize, hydrolysis post-heating makes enzyme deactivation;
(2) by hydrolyzed solution first through centrifuging ultrafiltration again, obtain filtrate;
(3) by filtrate reduced in volume, spraying dry, obtained yellowish and pale powder and soybean blood pressure lowering peptide.
Preferably, the alkali aqueous solution in step of the present invention (1) is the NaOH aqueous solution of concentration 10%, and add-on is soybean protein isolate weight is 5 times;
Preferred, the proteolysis enzyme concn in step of the present invention (1) is 50U/mL, and hydrolysis temperature is room temperature, and hydrolysis time is 3-5h, and heated and inactivated temperature is 70-80 DEG C;
Preferred further, the centrifugal rotational speed in step of the present invention (2) is 3000rpm, and ultra-filtration membrane aperture is 0.01-0.03 micron.
Preparation method of the present invention, with commercially available soybean protein isolate (protein content more than 87%) for raw material, adopt special protein enzymatic hydrolysis, through ultra-filtration and separation, remove unhydrolysed protein and macromole peptide, again through concentrated and spraying dry, obtained yellowish and pale powder and soybean blood pressure lowering peptide.
Soybean blood pressure lowering peptide of the present invention, has technique simple, workable, is applicable to suitability for industrialized production and the rate of recovery, quality product high.
Embodiment
Embodiment one:
Commercially available soybean protein isolate 10kg is joined in the NaOH aqueous solution of the concentration 10% of 50L and mix, add proteolytic ferment again and make its solubility 50U/mL, at room temperature be hydrolyzed 4h, then be heated to 75 DEG C and make enzyme deactivation, again hydrolyzed solution is first carried out the centrifuging that rotating speed is 3000rpm, carry out the ultrafiltration that aperture is 0.02 micron again, by the filtrate reduced in volume, the spraying dry that obtain, obtained yellowish and pale powder 570g and soybean blood pressure lowering peptide.
Embodiment two: the composition of soybean blood pressure lowering peptide
Soybean blood pressure lowering peptide composition prepared by the present invention as shown in Table-1
The composition of table l soybean blood pressure lowering peptide
As can be seen from table-1, the protein content of soybean blood pressure lowering peptide is up to 86.4%, substantially identical with former albumen; Acid-soluble egg, from up to 99.8%, is all almost small-molecular peptides that can be acid-soluble.Hazardous trace elements is all less than the foodstuff additive limit standard of national regulation.
Embodiment three: soybean blood pressure lowering peptide is to action effective, the agent effect of blood pressure
The molecular weight of the soybean blood pressure lowering peptide of preparation of the present invention, at about 1000Da, observes its blood pressure lowering effect to SHR rat by animal experiment.As can be seen from result, the hypotensive activity of soybean blood pressure lowering peptide clearly.Take medicine latter 3 days, experimental group blood pressure is namely than the low 30mm HR of control group; But from 10 days, experimental group blood pressure rose abreast together with control group blood pressure, this may be fixed with the unstable blood pressure of SHR rat own, prolongation in time and raise relevant.Dosage also has impact to blood pressure lowering effect.Low dosage can make blood pressure drops arrive lower value, but has bounce-back with ultimate effect; High dosage then can comparatively early reduce blood pressure, and keeps balance within the longer time.Soybean blood pressure lowering peptide prepared by visible the present invention is a kind of well material for lowering blood pressure.
Claims (4)
1. the preparation method of soybean blood pressure lowering peptide, comprises the following steps:
(1) mixed with alkali aqueous solution by commercially available soybean protein isolate, add proteolytic enzymes hydrolize, hydrolysis post-heating makes enzyme deactivation;
(2) by hydrolyzed solution first through centrifuging ultrafiltration again, obtain filtrate;
(3) by filtrate reduced in volume, spraying dry, obtained yellowish and pale powder and soybean blood pressure lowering peptide.
2. the preparation method of soybean blood pressure lowering peptide as claimed in claim 1, the alkali aqueous solution that it is characterized in that in described step (1) is the NaOH aqueous solution of concentration 10%, and add-on is soybean protein isolate weight is 5 times.
3. the preparation method of soybean blood pressure lowering peptide as claimed in claim 1 or 2, it is characterized in that the proteolysis enzyme concn in described step (1) is 50U/mL, hydrolysis temperature is room temperature, and hydrolysis time is 3-5h, and heated and inactivated temperature is 70-80 DEG C.
4. the preparation method of soybean blood pressure lowering peptide as claimed in claim 1, it is characterized in that the centrifugal rotational speed in described step (2) is 3000rpm, ultra-filtration membrane aperture is 0.01-0.03 micron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410423272.2A CN104232716A (en) | 2014-08-26 | 2014-08-26 | Soybean antihypertensive peptide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410423272.2A CN104232716A (en) | 2014-08-26 | 2014-08-26 | Soybean antihypertensive peptide and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN104232716A true CN104232716A (en) | 2014-12-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410423272.2A Pending CN104232716A (en) | 2014-08-26 | 2014-08-26 | Soybean antihypertensive peptide and preparation method thereof |
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| Country | Link |
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| CN (1) | CN104232716A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107007812A (en) * | 2017-04-05 | 2017-08-04 | 江苏大学 | A kind of method for extending captopril hypotensive efficacy time |
| CN111265649A (en) * | 2018-11-19 | 2020-06-12 | 林树芳 | Chinese herbal medicine blood pressure lowering medicine and preparation method thereof |
-
2014
- 2014-08-26 CN CN201410423272.2A patent/CN104232716A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107007812A (en) * | 2017-04-05 | 2017-08-04 | 江苏大学 | A kind of method for extending captopril hypotensive efficacy time |
| CN111265649A (en) * | 2018-11-19 | 2020-06-12 | 林树芳 | Chinese herbal medicine blood pressure lowering medicine and preparation method thereof |
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| C06 | Publication | ||
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Application publication date: 20141224 |