CN104222078B - A kind of for luring the compoistion and method of use of citrus psylla - Google Patents

Abstract

The present invention relates to orange disease and insect pests prevention and control field, specifically disclose a kind of for luring the compoistion and method of use of citrus psylla, described composition comprises γ-terpinene, australene and linalool, the composition prepared being inserted transparent plastic lures in worm bottle, cover bottle cap, spray insect sticker mythimna separata bottle luring outside worm bottle, host plant mythimna separata bottle being suspended to citrus psylla is used for traping and adheres to citrus psylla, described composition lures collection quantity can improve 49.3 times when citrus psylla density is high, lure collection quantity also can improve 5.17 times when citrus psylla density is low, its gathering effect is remarkable, can be used for control and prediction citrus psylla, prevent the citrus yellow shoot disease propagated by it simultaneously.

Description

Composition for attracting citrus psyllids and method of use thereof

technical field

The invention relates to the field of citrus pest control, and more particularly relates to a composition for attracting citrus psyllids and an application method thereof.

Background technique

The citrus psyllid Diaphorinacitri (Kuwayama) is the main pest of citrus at the shoot stage and the only vector of the most important disease of citrus - citrus greening disease. Citrus huanglongbing is the most dangerous infectious disease in the world's citrus industry. Since no effective method to control this disease has been found at home and abroad, the only way to prevent its spread is to control insect vectors in production. At present, chemical control of citrus psyllids is mainly adopted, that is, a large amount of chemical pesticides are sprayed every time the new shoots are sprouted. However, the use of chemical pesticides not only seriously pollutes the environment, but also causes pesticide residues, stimulates the outbreak of secondary pests, and produces pesticide resistance. Issues such as citrus affect the safe production of citrus and ultimately reduce the economic value of citrus.

Citrus psyllids have tenderness and yellowing properties. In the prior art, using yellow boards to lure citrus psyllids has certain effects, but the effect is still not significant. Therefore, more effective methods are sought to lure citrus psyllids. It is particularly important to prevent or forecast citrus Huanglongbing.

Plant secondary matter is produced by the plant's secondary metabolism and plays an important role in the plant's defense against its natural enemies, including pests. In the previous research, the inventor found that the young shoots of citrus infected with Huanglongbing yellowing have a significant effect on attracting them Enhancement, the present invention attempts to find the active ingredient formula for attracting citrus psyllids from the volatilized secondary metabolites after citrus is infected with Huanglongbing.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the insignificant effect of trapping citrus psyllids with yellow boards in the prior art, and to provide a composition for efficiently attracting citrus psyllids.

The second object of the present invention is to provide a method of using the above composition.

The third object of the present invention is to provide the application of the above composition in controlling citrus psyllid.

The purpose of the present invention is achieved through the following technical solutions:

A composition for attracting citrus psyllids is provided, the composition comprising gamma-terpinene and linalool.

Preferably, the volume ratio of γ-terpinene and linalool in the above composition is 2:3, and the balance is liquid paraffin solution, more preferably, the contents of the γ-terpinene and linalool are respectively 20ul/mL, 30ul/mL.

The citrus psyllid relies on the sense of smell and vision to locate the feeding direction. Its host has a unique volatile odor, which can play a role in attracting the citrus psyllid. Therefore, the trapping effect of the host plant odor can be fully used to control the citrus psyllid. , can not only reduce the extensive use of synthetic chemical pesticides and the negative impact on the environment, but also significantly reduce the resistance of pests, which is a promising technical method in the ecological control strategy of pests.

The volatile secondary metabolites of citrus include esters, aldehydes, sesquiterpenes, ethyl butyrate, etc. The composition of volatiles changes after being infected with Huanglongbing. Some studies have shown that the volatile species of young shoots of diseased trees with symptoms Among them, the contents of γ-terpinene and α-terpinolene were 1320% and 62% higher than normal young shoots, and the content of linalool was 356% higher than that of normal young shoots. Among them, linalool may be related to the preference of citrus psyllids for new shoots , so substances containing linalool can be used to lure citrus psyllids, but due to differences in sex, insects also have certain differences in the structure and number of odor recognition organs, which lead to their recognition and chemotactic responses to different odor substances Although the content of several substances in the infected citrus shoots is much higher than that of the corresponding substances in the normal citrus shoots, it is not the substances with increased levels that can attract citrus psyllids, the applicant In the early stage, a variety of volatile secondary metabolites in citrus were studied, and only some of them were found to be effective in attracting citrus psyllids.

On the basis of the disclosed prior art, the inventor conducted an experiment to lure citrus psyllids to various volatile substances present in citrus tender shoots, and found that mixing gamma-terpinene and linalool The obtained composition is more effective than linalool alone in attracting citrus psyllids. In particular, when the volume ratio of γ-terpinene and linalool was 2:3, the number of citrus psyllids attracted by it was 6.6 times that of linalool alone.

As a preferred technical solution, the above-mentioned composition of the present invention also includes α-pinene, that is, a composition formed by matching γ-terpinene, linalool and α-pinene in a certain ratio; the γ-terpinene The volume ratio of ene, α-pinene and linalool is 2:8:3, and the contents of the γ-terpinene, α-pinene and linalool are respectively 2～20ul/mL, 8～80ul/mL mL, 3~30ul/mL, the balance is liquid paraffin solution.

Provide a method for using any one of the above-mentioned compositions for attracting citrus psyllids, that is, drop 1 mL of the prepared composition on a cotton carrier, put it into a transparent plastic insect-attracting bottle, and cover the bottle cap. There is a vent hole between the mouth of the bottle, and then a layer of transparent or yellow sticky insect glue is evenly sprayed on the periphery of the insect trap bottle to make a transparent sticky insect bottle or a yellow sticky insect bottle, which is used to adhere to the trapped citrus psyllid. Hang the sticky bottle on the host plant of the citrus psyllid, and the sticky bottle is at the same height as the host plant or slightly lower than the height of the host.

The application of any one of the above compositions in preventing and treating citrus psyllid is provided.

Compared with the prior art, the present invention has the following beneficial effects:

The invention provides a composition for attracting citrus psyllids, which can increase the number of citrus psyllids by 49.3 times when the density of citrus psyllids is high, and attract the number of citrus psyllids when the density of citrus psyllids is low. It can also be increased by 5.17 times, and the trapping effect is remarkable. The composition of the invention can efficiently trap citrus psyllids and improve the control efficiency of citrus psyllids.

Description of drawings

Figure 1 is the trapping effect of different attractants on citrus psyllids on Murata; formula 1 is γ-terpinene (20ul/ml) + α-pinene (80ul/ml) + linalool (30ul /ml) liquid paraffin solution; Formula 2 is a liquid paraffin solution prepared from γ-terpinene (2ul/ml) + α-pinene (8ul/ml) + linalool (3ul/ml) ; Formula 3 is a liquid paraffin solution prepared from γ-terpinene (20ul/ml) + linalool (30ul/ml); the different lowercase letters above the columns in the figure indicate that the number of transparent sticky bottleworms is between Significant difference at 0.01 level; different capital letters indicate significant difference in the number of yellow armyworm bottleworms at 0.01 level;

Figure 2 is the trapping effect of different attractants on citrus psyllids on shatangju; formula 1 is γ-terpinene (20ul/ml) + α-pinene (80ul/ml) + linalool ( 30ul/ml) liquid paraffin solution; Formula 2 is a liquid paraffin solution prepared from γ-terpinene (2ul/ml) + α-pinene (8ul/ml) + linalool (3ul/ml) Solution; Formula 3 is a liquid paraffin solution prepared from γ-terpinene (20ul/ml) + linalool (30ul/ml); different lowercase letters above the columns in the figure indicate the number of transparent sticky bottleworms after analysis of variance There is a significant difference at the 0.01 level; different capital letters indicate a significant difference in the number of yellow armyworm bottleworms at the 0.01 level;

Fig. 3 is an effect diagram of trapping citrus psyllids in sticky insect bottles; wherein, Figure A is a transparent sticky insect bottle, and Figure B is a yellow sticky insect bottle.

Detailed ways

The present invention is further specifically described below by way of examples. Unless otherwise specified, the experimental methods used below are conventional methods in this technical field, and the ingredients or materials used, unless otherwise specified, are commercially available ingredients or materials.

Example 1

Attractant 1: 50ul/mL aromatic camphor, the balance is liquid paraffin solution.

Attractant 2: 30ul/mL camphor, 20ul/mLγ-terpinene, and the balance is liquid paraffin solution.

The above-mentioned two attractants were used on Murata to detect the attractant effect on citrus psyllids. The experimental method was as follows: the prepared attractant 1 and attractant 2 (the control was an equal amount of liquid paraffin solution) were respectively taken Drop 1mL onto the cotton carrier, put it into a transparent plastic insect-attracting bottle, cover the bottle cap, there is a vent hole between the bottle cap and the bottle mouth, and then evenly spray a layer of transparent or yellow viscous on the periphery of the insect-attracting bottle. Shellac, respectively made into a transparent sticky bottle or a yellow sticky bottle, used to adhere to the trapped citrus psyllids. Hang the sticky worm bottle on the mulberry, and the hanging bottle is at the same height as the host plant. The test results are shown in Table 1:

The results showed that the trapping effect of lure 2 was significantly higher than that of lure 1 after hanging the bottle for 10 days.

Example 2

The gamma-terpinene and aromatic camphor described in the attractant 2 in Example 1 are mixed in different proportions, and the balance is liquid paraffin solution to obtain the attractants of different proportions in the following table respectively. Said experimental method lures psyllids and statistical results are shown in Table 2:

The results show that when the volume ratio of γ-terpinene and camphor is 2:3 (the contents are 20ul/mL and 30ul/mL respectively), the trapping effect on psyllids is the best (as shown in Figure 1).

Example 3

The attractant described in this example is improved on the basis of attractant 2 in Example 1, namely including γ-terpinene, α-pinene and linalool.

The formula of the attractant described in this embodiment is: 2ul/mL, 8ul/mL, 3ul/mL, and the balance is liquid paraffin solution. Also using the experimental method described in Example 1, the citrus psyllid was lured with the attractant described in this example. The trapping effect of this lure on citrus psyllids on Jiulixiang:

Transparent sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 10.2 heads/bottle, which was extremely different from the liquid paraffin control (only 0.4 heads/bottle), which was 25.5 times that of the control.

Yellow sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 24 heads/bottle, which was significantly different from the liquid paraffin control (only 0.6 heads/bottle), which was 40 times that of the control (Figure 1).

Example 4

Adopt the technical scheme described in embodiment 3, the only difference is to detect the trapping effect of the hanging bottle on the shatangju orchard to the citrus psyllid, and the detection results are as follows:

Transparent sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 2.25 heads/bottle, which was significantly different from the liquid paraffin control (only 0.50 heads/bottle), which was 4.5 times that of the control.

Yellow sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 2.75 heads/bottle, which was significantly different from the liquid paraffin control (only 0.75 heads/bottle), which was 3.67 times that of the control (as shown in Figure 2).

Example 5

In this embodiment, the attractant described in Example 3 is further improved, that is, the formula of the attractant is: 20ul/mL, 80ul/mL, 30ul/mL, and the balance is liquid paraffin solution.

Also using the experimental method described in Example 1, the citrus psyllid was lured with the attractant described in this example. The trapping effect of this lure on citrus psyllids on Jiulixiang:

Transparent sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 14 heads/bottle, which was extremely different from the liquid paraffin control (only 0.4 head/bottle), which was 35 times that of the control.

Yellow sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 29.6 heads/bottle, which was significantly different from the liquid paraffin control (only 0.6 heads/bottle), which was 49.3 times that of the control (as shown in Figure 1) .

Example 6

Adopt the technical scheme described in embodiment 5, the only difference is to detect the trapping effect of the hanging bottle on the shatangju orchard to the citrus psyllid, and the detection results are as follows:

Transparent sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 3 heads/bottle, which was extremely different from the liquid paraffin control (only 0.50 heads/bottle), which was 6 times that of the control.

Yellow sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 3.88 heads/bottle, which was significantly different from the liquid paraffin control (only 0.75 heads/bottle), which was 5.17 times that of the control (as shown in Figure 2).

Example 7

In this example, further improvements are made to the attractant described in Example 3, that is, the formula of the attractant is: 10ul/mL, 40ul/mL, 15ul/mL, and the balance is liquid paraffin solution.

Also using the experimental method described in Example 1, the citrus psyllid was lured with the attractant described in this example. The trapping effect of this lure on citrus psyllids on Jiulixiang:

Transparent sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 11.8 heads/bottle, which was significantly different from the liquid paraffin control (only 0.4 heads/bottle), which was 29.5 times that of the control.

Yellow sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 26.2 heads/bottle, which was extremely different from the liquid paraffin control (only 0.6 heads/bottle), which was 43.7 times that of the control.

Example 8

Adopt the technical scheme described in embodiment 7, the only difference is to detect the trapping effect of the hanging bottle on the shatangju orchard to the citrus psyllid, and the detection results are as follows:

Transparent sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 1.8 heads/bottle, which was significantly different from the liquid paraffin control (only 0.50 heads/bottle), which was 3.6 times that of the control.

Yellow sticky insect bottle: The number of citrus psyllids trapped after hanging the bottle for 10 days was 3.4 heads/bottle, which was significantly different from the liquid paraffin control (only 0.75 heads/bottle), which was 4.5 times that of the control.

Claims (5)

1. lure a composition for citrus psylla, it is characterized in that, comprise γ-terpinene and linalool; The volume ratio of described γ-terpinene and linalool is 2:3.

2. composition according to claim 1, is characterized in that, also comprises australene; The volume ratio of described γ-terpinene, australene and linalool is 2:8:3.

3. composition according to claim 1 and 2, is characterized in that, described γ-terpinene content is in the composition 2 ~ 20ul/mL.

4. the using method of composition described in any one of claim 1 to 2, it is characterized in that, the composition prepared is dripped on cotton carrier, inserting transparent plastics lures in worm bottle, cover bottle cap, between described bottle cap and bottleneck, have air-vent, lure at plastics and spray insect sticker outside worm bottle and make mythimna separata bottle, mythimna separata bottle is suspended on the host plant of citrus psylla, described mythimna separata bottle and host plant is contour or a little less than the height of host plant.

5. the application of composition described in any one of claim 1 to 2 in control citrus psylla.