CN104222013B

CN104222013B - A kind of method of a large amount of production Phyloseiulus nersimilis

Info

Publication number: CN104222013B
Application number: CN201310255787.1A
Authority: CN
Inventors: 王伯明; 徐学农; 姜晓环; 王恩东
Original assignee: Shoubonong Beijing Biotechnology Co ltd; Institute of Plant Protection of CAAS
Current assignee: Shoubonong Beijing Biotechnology Co ltd; Institute of Plant Protection of CAAS
Priority date: 2013-06-21
Filing date: 2013-06-21
Publication date: 2016-09-07
Anticipated expiration: 2033-06-21
Also published as: CN104222013A

Abstract

本发明涉及一种大量生产智利小植绥螨的方法，其特征是包括叶螨的饲养方法、智利小植绥螨的首次饲养和连续饲养方法、智利小植绥螨的分离收集方法、智利小植绥螨的包装方法及种植基质的处理和重新利用方法五部分。本发明省去了现有智利小植绥螨饲养方法中的多步工序，不仅能够进一步降低15％以上的饲养成本、单位面积智利小植绥螨的生产量由10000头/平方米可提升到30000头/平方米，而且首次提出连续饲养智利小植绥螨时无需接种也可实现其规模化大量饲养的方法，以及分离、收集和包装智利小植绥螨的方法和节约利用种植基质的办法。The present invention relates to a method for mass production of Phytoseiillus militaris, which is characterized in that it comprises a method for raising spider mite, a method for first raising and continuous raising of Phytoseiillus militaris, a method for separating and collecting Phytoseiili militaris, The packaging method of phytoseiid mite and the treatment and reuse method of planting substrate are five parts. The present invention saves the multi-step process in the existing phytoseiid mite raising method, not only can further reduce the feeding cost by more than 15%, the production capacity of phytoseiid mite per unit area can be increased from 10000 heads/square meter to 30,000 heads/square meter, and for the first time, it is the first time to propose a method for large-scale mass breeding of Phytoseiillus militaris without inoculation, a method of isolating, collecting and packaging Phytoseioid militaris and a method of saving and using planting substrates .

Description

A kind of method of a large amount of production Phyloseiulus nersimilis

Technical field

The present invention relates to the method that one produces Phyloseiulus nersimilis (Phytoseiulus persimilis) in a large number, belong to agricultural Biological control of insect pests field.

Background technology

Phyloseiulus nersimilis is not only both at home and abroad for preventing and treating the Important Natural Enemy of greenhouse tetranychid, or the special feeding habits predator of tetranychid.

At present, the method for breeding of Phyloseiulus nersimilis is still only with tetranychid mass rearing, and existing Phyloseiulus nersimilis is raised Although method is capable of the scale mass rearing of Phyloseiulus nersimilis, but there is also feeding cost height, Phyloseiulus nersimilis list Plane amasss production scale than relatively low defect, and the problem that the separation of Phyloseiulus nersimilis, collection and packing technique are the best.

How to reduce feeding cost, increase unit are Phyloseiulus nersimilis output, solve Phyloseiulus nersimilis separation, Collect and packaging Problems needs to study.

Summary of the invention

It is an object of the invention to provide one can reduce further Phyloseiulus nersimilis feeding cost, increase unit are Chile little The output of phytoseiid mite, solution Phyloseiulus nersimilis separate, collect and the mass production method of packaging Problems.Before comparing the present invention Phyloseiulus nersimilis method for breeding, the present invention can not only reduce the feeding cost of more than 15%, and unit are Chile is little The output of phytoseiid mite also can be risen to 30000/square metre by 10000/square metre；Meanwhile, present invention also offers one Plant the preferred plan separating, collecting Phyloseiulus nersimilis, make operation easier；In terms of packaging, the present invention uses bottled, it is to avoid Packed Phyloseiulus nersimilis there may be the problem of extruding, makes the relatively expensive Phyloseiulus nersimilis safer.

The present invention is that the host plant first with tetranychid raises tetranychid, and the tetranychid that recycling is raised raises the side of Phyloseiulus nersimilis Method, but in key operation, not only have the biggest difference, but also provide a kind of preferably collection and packing method, specifically crucial Operating procedure is:

1) raising of tetranychid: in host plant launches the first 0.5 day-15 days to true leaf, by the blade with tetranychid kind source Or plants stems is inoculated on new host plant together with the composition of blade, the tetranychid in inoculation is each mite state of tetranychid, described leaf Sheet is not cut into small pieces or is seldom cut into small pieces, and wherein new host plant width of blade is at least at more than 1cm, if host plant is dish Beans then preferred host plant leaf blade mean breadth reaches inoculation during more than 6cm, and the tetranychid amount that average every strain host plant is accessed is more than 5, preferably more than 30, remove in 0.1 day-10 days after inoculation for new host plant inoculation blade or stem and The composition of blade, wherein the terminal bud to plant processes is not cut or cut less, the most only cuts 1-2 time.

Wherein, the composition of described plants stems and blade refer to for tetranychid inoculation carrier in the most singly have blade also have plants stems or Side shoot on stem, branch, palpus branch；Described blade is seldom cut into small pieces and refers to that blade is completely or partially complete, and imperfect Part be when shearing with the plant of tetranychid, may incidentally miscut or accidentally injure the blade fritter that blade is formed；Described terminal bud Process is not cut or cut less refer to not cut terminal bud or cut terminal bud rather than always once find that terminal bud is the most several times less Wipe out, need member-retaining portion terminal bud or all terminal bud continued growths.

2) raising of Phyloseiulus nersimilis: the whole receptacle raising Phyloseiulus nersimilis is to seal, but can breathe freely, and is raising During supporting Phyloseiulus nersimilis, it is not necessary to dependent insect cage.

The method for breeding first of Phyloseiulus nersimilis: during raising Phyloseiulus nersimilis, the inoculation of Phyloseiulus nersimilis carries Body is not essential, if having, inoculation carrier can be the most singly blade, if selection blade is as carrier, and preferably plants stems and blade Composition as the inoculation carrier of Phyloseiulus nersimilis；Phyloseiulus nersimilis is accessed when every strain tetranychid amount reaches more than 100 Each mite state, preferably every strain tetranychid amount reaches to access Phyloseiulus nersimilis when more than 500；Tetranychid and the access of Phyloseiulus nersimilis Ratio is more than 5: 1, preferably greater than 50: 1, less than 1000: 1；Raise nothing between the host plant planting container of Phyloseiulus nersimilis Need or less with boom, preferably without boom；Terminal bud process to plant is not cut or cut less, does not cuts.

Next batch and the method raising Phyloseiulus nersimilis the most continuously, method for breeding can be with the head of above-mentioned Phyloseiulus nersimilis Secondary method for breeding is identical, but preferably the following stated method for breeding, concrete operations are: after last batch Phyloseiulus nersimilis is gathered, The new a collection of host plant with tetranychid, Phyloseiulus nersimilis to be seeded is not to continue with using inoculation Phyloseiulus nersimilis to host Method mass rearing Phyloseiulus nersimilis on plant, but after last batch Phyloseiulus nersimilis is gathered within 10 days, preferably In 1 day, host plant to be seeded is moved in the receptacle of the complete Phyloseiulus nersimilis of freshly harvested, be placed on raising frame, raising Platform, ground, the complete Phyloseiulus nersimilis of freshly harvested remaining host plant plantation utensil, planting matrix or other can see Chile little Each of phytoseiid mite is local, removes the host plant plantation utensil that last batch stays, the most again put after 0.1 day-10 days Or the host plant that transfer stays, determine whether directly to gather or mend again to fill out portion according to the amount of Phyloseiulus nersimilis on host plant Point with the host plant of tetranychid or continue to raise or mend again to put to continue after part Phyloseiulus nersimilis to raise, but the most no longer Benefit puts Phyloseiulus nersimilis.

Wherein, the composition of described plants stems and blade refers to the most singly have blade to also have in the carrier of Phyloseiulus nersimilis inoculation Side shoot in plants stems or stem, branch, palpus branch；It is not cut or cut less to refer to not cut terminal bud or few that described terminal bud processes Cut terminal bud rather than always once find that terminal bud is wiped out the most in time several times, need member-retaining portion terminal bud or all terminal bud continued growths； Inoculation not essential the referring to of carrier of described Phyloseiulus nersimilis can inoculate the pure Phyloseiulus nersimilis without carrier, it is also possible to connects Plant the Phyloseiulus nersimilis with carrier；Described single can be that blade refers to that the inoculation carrier of Phyloseiulus nersimilis can be to appoint The carrier of what carrying Phyloseiulus nersimilis；Described plantation utensil refers to for planting the basin of host plant, dish, container etc., these Being filled with planting matrix in utensil, host plant is grown in these planting matrixs.

3) collection method of Phyloseiulus nersimilis: when the Phyloseiulus nersimilis number of animals raised reaches the amount of gathering, so that it may to Phyloseiulus nersimilis Carrying out gathering, collecting, collection method is to cut host plant with scissors, and then plants stems, branch are put into together with leaf collection dress In putting, it is possible to individually blade or the branches and leaves of clip host plant are put in collection device, seal collection device the most as early as possible, then will Collection device is placed in suitable space, fixes the collection that i.e. can be used for Phyloseiulus nersimilis each movable mite state after putting well, collects behaviour Work is every weekly check, collects more than 1 time, preferably checks every day, collects more than 1 time, until collecting pipe is collected little less than Chile Till phytoseiid mite, collecting pipe is that length is within 1m, for collecting the container of Phyloseiulus nersimilis, it is ensured that collect little less than Chile Plants stems, branch or the leaf in collection device can be cleared up after phytoseiid mite.

Wherein, described collection device refers to the utensil for collecting Phyloseiulus nersimilis, and collection device can be made up of two parts also may be used Being made up of three parts, preferably collection device is made up of three parts, if collection device is made up of two parts, including collecting container and receipts Collector two parts, when collecting Phyloseiulus nersimilis, directly and coated lycopene, Phyloseiulus nersimilis is to hold from collecting to collecting pipe Directly climb in collecting pipe in device；If collection device is made up of three parts, including collecting container, being contained in the collection collected on container Conduit and collecting pipe three part, when collecting Phyloseiulus nersimilis, collecting pipe is directly connected with collecting duct, and Phyloseiulus nersimilis is Climb to again in collecting pipe in first climbing to collecting duct in collecting container；

Described collection device is placed on suitable space and refers to be designed for the space that Phyloseiulus nersimilis is collected, it is desirable to this space is in intelligence Profit little phytoseiid mite has light unglazed when collecting, but changes in collecting pipe, cleaning collection device in fixing collection device, inspection Light is needed when plants stems, branch or leaf；

Described collection container refers to for loading Phyloseiulus nersimilis and the part of plants stems, branch or leaf, collects container and has 0.001m³-1m³Volume, collect the manufacture material of container must contain gauze, gauze, cloth, fly net, paper or other can breathe freely and can Effectively stop the material that Phyloseiulus nersimilis is escaped, described gauze, gauze, cloth, fly net, paper or other can breathe freely and can have But effect stops the material of Phyloseiulus nersimilis escape to can be used alone also, and two or more is used in mixed way, and collects container and also must Must have preferable sealing, it is possible to ensure that Phyloseiulus nersimilis cannot climb out of from other place in addition to climbing in collecting pipe as far as possible, Collect container and also there is the opening being easily opened and sealing, open opening and can load Phyloseiulus nersimilis and plants stems, branch or leaf, Seal opening the most salable collection container, wherein collect on container for loading Phyloseiulus nersimilis and plants stems, branch, the opening of leaf The opening that mouth climbs out of in collecting container more than Phyloseiulus nersimilis.

Described collecting duct refers to be contained in collects the passage climbed out of on container for Phyloseiulus nersimilis, and Phyloseiulus nersimilis can be from Collecting duct is contained in that collect on container and climbs out of collection container, then connect together from collecting duct and collecting pipe that Crawling into collecting pipe, the collecting duct number collected on container is one or more, and the length of collecting duct is within 1m.

4) packing method of Phyloseiulus nersimilis: after utilizing collection device to collect Phyloseiulus nersimilis, can directly pack and also may be used Packing the when of first refrigerating to be needed, packing method is by packaging standard amount, puts into Phyloseiulus nersimilis with vermiculite or wood again In the Packaging Bottle of bits or vermiculite and wood chip mixture, the most quickly cover tightly bottle cap, it is desirable to Packaging Bottle body and bottle cap can be the closeest Sealing, can effectively stop mite class to be escaped, Phyloseiulus nersimilis can apply or continue refrigeration after having packed, refrigerated storage temperature is 0 DEG C-20 DEG C, preferably 3 DEG C-8 DEG C, described Packaging Bottle bottle cap is with air-vent, and air-vent size, shape are not fixed, but air-vent Need to be sealed by paper or gauze, gauze, cloth, preferably be sealed by paper.

5) process of planting matrix and re-using: first remove used planting matrix in tetranychid or Phyloseiulus nersimilis receptacle Matter, it is possible to the plantation utensil being used in connection with removes receptacle together, after removing or pour out used planting matrix, places 0.1 More than it, it is continuing with after directly sieving or smash with pulverizer after used planting matrix certain mass dryness fraction to be achieved, Qi Zhongruo Sieving, screen sizes is 0.1cm-10cm, screen cloth planting matrix can be allowed to pass through and can prevent host plant root, stem, leaf or its Its foreign material passes through, and the root, stem and leaf of host plant can be continuing with after smashing in pulverizer or directly abandon, after sieving or after smashing The planting matrix stayed can directly utilize or be used in mixed way with new planting matrix or add other and be conducive to what host plant grew to become Point, but preferably through freezing, high temperature deinsectization sterilizing or process of being exposed to the sun before utilizing these used planting matrixs, to guarantee to plant Plant Medium Culture and there is no Phyloseiulus nersimilis, tetranychid or other insects, pathogenic bacteria etc..Wherein, described plantation utensil refers to for planting Planting the basin of host plant, dish, container etc., be filled with planting matrix in these utensils, host plant is grown in these planting matrixs In.

The present invention compared with prior art, in terms of the method for breeding of tetranychid and Phyloseiulus nersimilis: except access tetranychid, Chile little Outside the mode of phytoseiid mite, time, amount, ratio etc. are different, also reside in no matter the present invention is raising tetranychid or raising intelligence Be all that the terminal bud to plant processes during the little phytoseiid mite of profit it is not cut or cut less, is raising nothing during Phyloseiulus nersimilis Need or use less boom, and propose the method raising Phyloseiulus nersimilis continuously first, such that it is able to avoid having been used up inoculation Mode raises the method for Phyloseiulus nersimilis, reduces the most again feeding cost；Separate and in terms of collection at Phyloseiulus nersimilis, Present invention firstly provides a kind of new collection device, this device all can realize the receipts of Phyloseiulus nersimilis in the case of having light unglazed Collection, wherein collecting container is not the most to use plastic sheeting, it is also possible to completely without reinforcing bar or reinforcing bar, without support, collect and hold For loading Phyloseiulus nersimilis and plants stems on device, branch, the opening of leaf climb out of in collecting container more than Phyloseiulus nersimilis Opening, comparing lower the actual collecting effect of collection method can be higher, and cost is lower, reduce by 10% the most again, is more suitable for Chile little A large amount of collections of phytoseiid mite；In terms of packaging, the present invention uses bottled, it is to avoid packed Phyloseiulus nersimilis there may be extruding Problem, make relatively expensive Phyloseiulus nersimilis safer；In terms of the re-using of planting matrix, the present invention is first The secondary method proposing to re-use these planting matrixs, it is possible to more effectively save and utilize resource.

Present invention eliminates the multistep operation in existing Phyloseiulus nersimilis method for breeding, more than 15% can not only be reduced further Feeding cost, the output of unit are Phyloseiulus nersimilis can be risen to 30000/square metre by 10000/square metre, And the method that can realize its scale mass rearing without inoculation when raising Phyloseiulus nersimilis continuously, Yi Jifen are proposed first From, collect and pack the method for Phyloseiulus nersimilis and saving utilizes the way of planting matrix.

Detailed description of the invention

Following methods is only the citing of the embodiment to claim and summary of the invention, does not represent present claims and summary of the invention Restriction.

The method for breeding of embodiment 1 tetranychid

Raising indoor feeding tetranychid between temperature 15 DEG C-45 DEG C, humidity 50%-100%, first plants host plant, host Plant is launched first in 0.5 day-15 days of true leaf, by with the blade in tetranychid kind source or the composition one of plants stems and blade Rising and be inoculated on new host plant, the tetranychid in inoculation is each movable mite state of tetranychid, and described blade is not cut into small pieces or seldom cuts Becoming fritter, wherein new host plant width of blade is at least at more than 1cm, if host plant is Kidney bean, and preferred host plant leaf blade Mean breadth reaches inoculation during more than 6cm, and the tetranychid amount that average every strain host plant is accessed is more than 5, preferably more than 30, The blade or stem and the composition of blade inoculated for new host plant is removed in 0.1 day-10 days after inoculation, the most right It is not cut or cut less that the terminal bud of plant processes, and the most only cuts 1-2 time.

The method for breeding first of embodiment 2 Phyloseiulus nersimilis

Raising indoor feeding Phyloseiulus nersimilis between temperature 15 DEG C-45 DEG C, humidity 50%-100%, raise Chile little plant peaceful The whole receptacle of mite is to seal, but can breathe freely, during raising Phyloseiulus nersimilis, it is not necessary to dependent insect cage.

During raising Phyloseiulus nersimilis, the inoculation carrier of Phyloseiulus nersimilis is not essential, if having, inoculation carrier is not List can be blade, if selection blade is as carrier, and preferably plants stems and composition the connecing as Phyloseiulus nersimilis of blade Plant carrier；Access Phyloseiulus nersimilis when every strain tetranychid amount reaches more than 100, preferably every strain tetranychid amount reach 500 with Phyloseiulus nersimilis was accessed time upper；The access ratio of tetranychid and Phyloseiulus nersimilis is more than 5: 1, preferably greater than 50: 1, is less than 1000∶1；Raise between the host plant planting container of Phyloseiulus nersimilis without or less with boom, preferably without boom；To planting It is not cut or cut less that the terminal bud of strain processes, and does not cuts.

Wherein, the composition of described plants stems and blade refers to the most singly have blade to also have in the carrier of Phyloseiulus nersimilis inoculation Side shoot in plants stems or stem, branch, palpus branch；It is not cut or cut less to refer to not cut terminal bud or few that described terminal bud processes Cut terminal bud rather than always once find that terminal bud is wiped out the most in time several times, need member-retaining portion terminal bud or all terminal bud continued growths； Inoculation not essential the referring to of carrier of described Phyloseiulus nersimilis can inoculate the pure Phyloseiulus nersimilis without carrier, it is also possible to connects Plant the Phyloseiulus nersimilis with carrier；Described single can be that blade refers to that the inoculation carrier of Phyloseiulus nersimilis can be to appoint The carrier of what carrying Phyloseiulus nersimilis.

The continuous method for breeding of embodiment 3 Phyloseiulus nersimilis

After last batch Phyloseiulus nersimilis is gathered, new is a collection of with tetranychid, the host plant of Phyloseiulus nersimilis to be seeded It is not to continue with the method mass rearing Phyloseiulus nersimilis using inoculation Phyloseiulus nersimilis to host plant, but at last consignment of Within secondary Phyloseiulus nersimilis is gathered latter 10 days, in preferably 1 day, host plant to be seeded is moved into that the complete Chile of freshly harvested is little plants Pacify in the receptacle of mite, be placed on raising frame, raise platform, ground, the complete Phyloseiulus nersimilis of freshly harvested remaining host plant kind Plant utensil, planting matrix or other can see that each of Phyloseiulus nersimilis is local, remove last batch after 0.1 day-10 days and stay Host plant plantation utensil, the most again put or shift the host plant stayed, according on host plant Chile little plant peaceful The amount of mite determines whether directly to gather or mends and fills out part and with the host plant of tetranychid or continue to raise or mend to put part Continue after Phyloseiulus nersimilis to raise, but the most no longer mend and put Phyloseiulus nersimilis.Wherein, described plantation utensil refers to for planting Planting the basin of host plant, dish, container etc., be filled with planting matrix in these utensils, host plant is grown in these planting matrixs In.

The collection method of embodiment 4 Phyloseiulus nersimilis

When the Phyloseiulus nersimilis number of animals raised reaches the amount of gathering, so that it may gather Phyloseiulus nersimilis, collect, collection method is Cut host plant with scissors, then plants stems, branch are put in collection device together with leaf, it is possible to individually clip host plant Blade put in collection device, seal collection device the most as early as possible, then collection device be placed in suitable space, fixing put I.e. can be used for the collection of Phyloseiulus nersimilis each movable mite state after Hao, collect operation and be every weekly check, collect more than 1 time, preferably Every day check, collect more than 1 time, until collecting pipe collect less than Phyloseiulus nersimilis till, collecting pipe be length within 1m, For collecting the container of Phyloseiulus nersimilis, it is ensured that collect less than the plants stems can cleared up after Phyloseiulus nersimilis in collection device, Branch or leaf.

Wherein, described collection device is the utensil for collecting Phyloseiulus nersimilis, and collection device can be made up of two parts also can be by Three part compositions, preferably collection device is made up of three parts, if collection device is made up of two parts, including collecting container and collection Pipe two parts, when collecting Phyloseiulus nersimilis, directly and coated lycopene, Phyloseiulus nersimilis is from collecting container to collecting pipe The most directly climb in collecting pipe；If collection device is made up of three parts, lead including the collection collecting container, be contained on collection container Pipe and collecting pipe three part, collect Phyloseiulus nersimilis time, collecting pipe is directly connected with collecting duct, Phyloseiulus nersimilis be from Climb to again in collecting pipe in first climbing to collecting duct in collecting container；

Described collection device is placed on suitable space and refers to be designed for the space that Phyloseiulus nersimilis is collected, it is desirable to this space is in intelligence Profit little phytoseiid mite has light unglazed when collecting, but changes in collecting pipe, cleaning collection device in fixing collection device, inspection Need light when plants stems, branch or leaf, collecting chamber temperature 15 DEG C-45 DEG C, humidity is between 30%-100%；

Described collection container refers to for loading Phyloseiulus nersimilis and the part of plants stems, branch or leaf, collects container and has 0.001m³-1m³Volume, its surface area more than 10%, preferably 50% be above being made up of gauze, gauze, cloth, fly net or paper, receives Collection container must also have preferable sealing, it is possible to ensure that Phyloseiulus nersimilis cannot be from it in addition to climbing in collecting pipe as far as possible Its place climbs out of, and collects container and also has the opening being easily opened and sealing, opens opening and can load Phyloseiulus nersimilis and plant Strain stem, branch or leaf, seal opening the most salable collection container, wherein collect on container and be used for loading Phyloseiulus nersimilis and plant The opening that stem, branch, the opening of leaf climb out of in collecting container more than Phyloseiulus nersimilis.

Described collecting duct refers to be contained in the passage climbed out of for Phyloseiulus nersimilis collected on container, and Phyloseiulus nersimilis is permissible Climb out of from being contained in one, the conduit collected container, then crawl into collecting pipe from the other end connected together with collecting pipe, collect container On collecting duct number be one or more, the length of collecting duct is within 1m；

The packing method of embodiment 5 Phyloseiulus nersimilis

After utilizing collection device to collect Phyloseiulus nersimilis, can directly pack the when of also can first refrigerating to be needed and pack again, Packing method is by packaging standard amount, Phyloseiulus nersimilis is put into the Packaging Bottle with dry or wet vermiculite, the most quickly covers tightly Bottle cap, it is desirable to Packaging Bottle body and bottle cap can fully seal, can effectively stop mite class to be escaped, and Phyloseiulus nersimilis has been packed After can apply or continue refrigeration, refrigerated storage temperature is at 0 DEG C-20 DEG C, preferably 3 DEG C-8 DEG C, and described Packaging Bottle bottle cap is with ventilative Hole, air-vent size, shape do not fix, but air-vent need to be sealed by paper or gauze, gauze, cloth, is preferably sealed by paper.

The process of embodiment 6 planting matrix and re-using

First in tetranychid or Phyloseiulus nersimilis receptacle, remove used planting matrix, it is possible to the plantation utensil being used in connection with Removing receptacle together, after removing or pour out used planting matrix, place more than 0.1 day, used planting matrix is treated Being continuing with after directly sieving or smash with pulverizer after reaching certain mass dryness fraction, if wherein sieving, screen sizes is 0.1cm-8cm, Screen cloth can allow planting matrix to pass through and can prevent host plant root, stem, leaf or other foreign material from passing through, the root, stem and leaf of host plant Can be continuing with after smashing in pulverizer or directly abandon, the planting matrix stayed after sieving or after smashing can directly utilize or with New planting matrix is used in mixed way or adds other composition being conducive to host plant to grow, but is utilizing these used planting matrixs Preferably through freezing, high temperature deinsectization sterilizing or process of being exposed to the sun before matter, there is no Phyloseiulus nersimilis, tetranychid in guaranteeing planting matrix Or other insects, pathogenic bacteria etc..Wherein, described plantation utensil refers to for planting the basin of host plant, dish, container etc., this Being filled with planting matrix in a little utensils, host plant is grown in these planting matrixs.

Claims

1. A method for mass production of Phytoseiulus persimilis (Phytoseiulus persimilis), characterized in that it utilizes the host plant of Tetranychus persimilis to raise Tetranychus, then utilizes the method of raised Tetranychus to raise Phytoseiulus persimilis, specific key The operation steps are:

1) Feeding of spider mites: within 0.5 days to 15 days after the host plant unfolds the first pair of true leaves, the leaves with the source of spider mites or the composition of the plant stem and leaves are inoculated on the host plant together, and the inoculated Tetranychus are various forms of Tetranychus, and the leaves are not cut into small pieces or rarely cut into small pieces, wherein the width of the leaves of the host plant is more than 1 cm, and the average number of spider mites inserted into each host plant is more than 5, Within 10 days after inoculation, remove the leaves or the combination of plant stems and leaves used for host plant inoculation, wherein the terminal buds of the plants are not cut or cut less;

2) Breeding of Phytoseiillus militaris: The whole breeding room for raising Phytoseiillus militaris is airtight, and in the process of raising Phytoseiillus militaris, no insect cage is needed;

The first feeding method of Phytoseiillus militaris: In the process of raising Phytoseiillus militaris, the inoculation carrier of Phytoseiillus militaris is optional. When the number of phytoseiid mites reached more than 100, each mite state of Phytoseiid militaris was inoculated, and the inoculation ratio of spider mites and Phytoseioid militaris was greater than 5:1, and no or less water was needed between the planting containers of the host plants for raising Phytoseioid militaris Fence, the treatment of the terminal buds of the plants is not to cut or to cut less;

The method for continuously raising Phytoseiillus militaris in the next batch and later, the feeding method is the same as the first feeding method of Phytoseiillus militaris above, or choose the following feeding method, the specific operation is: wait for the last batch of Phytoseioid militaris After the mites were harvested, a new batch of host plants with spider mites and to be inoculated with Phytoseiillus paliscens did not continue to use the method of inoculating Phytoseius paliscens to host plants to raise Phytoseius paliscens in large quantities, but in Within 10 days after the harvest of the last batch of Phytoseiid mites, the host plants to be inoculated were moved into the rearing room where Phytoseiid mites had just been harvested, and placed on the rearing racks, breeding tables, grounds, and freshly harvested plants. The remaining host plant planting utensils, planting substrates, or other places where Phytoseiin militaris can be seen, remove the host plant planting utensils left over from the previous batch after 0.1 days to 10 days, and then Rearrange or transfer the remaining host plants, and judge whether to harvest them directly, or fill in some host plants with spider mites, continue to raise them, or replant some Continue to raise after Phytoseiillus militaris;

3) Separation and collection method of Phytoseius militaris: when the feeding amount of Phytoseioid militaris reached the harvesting amount, Phytoseiilis militaris could be harvested and collected. The collection method was to cut off the host plant with scissors, and then Put the stems, branches and leaves of the plant into the collection device together, or cut the leaves or branches of the host plant separately and put them into the collection device, then seal the collection device as soon as possible, then put the collection device in a suitable space, and place it in a fixed place. After being ready, it can be used for the collection of the various active mite states of Phytoseiillus militaris; the collection device refers to the utensil used to collect Phytoseioid militaris, and the collection device can be composed of two parts or three parts. If the collection device consists of The two parts consist of a collection container and a collection tube. When collecting Phytoseioid mites, the collection pipe is directly connected to the collection container, and Phytoseioid mites climb directly into the collection pipe from the collection container; The part consists of three parts: a collection container, a collection conduit mounted on the collection container, and a collection pipe. When collecting Phytoseioid mites, the collection pipe is directly connected to the collection conduit. Phytoseioids is first crawled from the collection container Climb into the collection tube and then climb into the collection tube; the collection operation is to check and collect more than once a week until the collection tube cannot collect Phytoseioid mites. Clean up the plant stems, branches or leaves in the collection device after ensuring that no phytoseiid mites are collected;

4) Packing method of Phytoseius paliscens: after collecting Phytoseius paliscens with a collection device, it can be packed directly or refrigerated and repacked when needed. Put the mites into a packaging bottle with vermiculite or wood chips or a mixture of vermiculite and wood chips, and then quickly close the bottle cap tightly. It is required that the bottle body and bottle cap of the packaging bottle can be completely sealed, which can effectively prevent mites from escaping. After packaging, it can be used or continued to be refrigerated. The refrigerated temperature is 0°C-20°C. The bottle cap of the packaging bottle has air holes. The size and shape of the air holes are not fixed, but the air holes need to be covered with paper or gauze, cloth seal;

5) Treatment and reuse of planting substrate: first remove the used planting substrate from the breeding room of spider mites or phytoseiillus chileans, or move it out of the breeding room together with the used planting utensils, remove or pour out the used planting After the substrate, place it for more than 0.1 day. After the used planting substrate reaches a certain dryness, it will be sieved directly or crushed with a pulverizer before continuing to use. It can allow the planting substrate to pass through and prevent the host plant roots, stems, leaves or other debris from passing through. The roots, stems and leaves of the host plant can be crushed in the pulverizer and then used or directly discarded. The planting medium can be used directly or mixed with new planting medium or added with other ingredients that are beneficial to the growth of the host plant. However, before using these used planting mediums, they must be frozen, sterilized at high temperature or exposed to the sun to ensure that the planting medium is healthy and healthy. There are no phytoseiid mites, spider mites or other insects and pathogenic bacteria in it.

2. according to claim 1, a kind of method of mass-producing Phytoseiid militaris is characterized in that the composition of said plant stem and blade refers to not only The leaves also have plant stems or side shoots, branches, and fibrous branches on the stems.

3. according to claim 1, a kind of method for mass production of Phytoseius militaris, is characterized in that described blade is seldom cut into fritters and means that all or part of blade is complete, and the incomplete part is in the process of cutting When cutting plants with spider mites, there may be small pieces of leaves formed by mistakenly cutting or accidentally injuring leaves.

4. according to claim 1, a kind of method of producing Phytoseius militaris in large quantities, it is characterized in that described terminal bud treatment is not cut or cut less and refers to can not cut terminal bud at all or cut terminal bud several times less, Instead of cutting off the terminal buds in time once they are found all the time, it is necessary to keep some or all of the terminal buds to continue to grow.

5. according to claim 1, a kind of method for mass production of Phytoseiillus paliscens is characterized in that the inoculation carrier of said Phytoseius paliscens is dispensable and refers to the pure Phytoseius palisei that can be inoculated without carrier mites, can also be inoculated with the carrier Phytoseiid militaris.

6. according to claim 1, a kind of method for mass production of Phytoseiillus papillonus, it is characterized in that the described not only can be only the leaf means that the inoculation carrier of Phytoseius papillonus can be any carrier that carries Phytoseioid papillonus .

7. according to claim 1, a kind of method of mass-producing phytoseiid mites is characterized in that said planting utensils refer to containers for planting host plants, and these utensils are filled with planting substrates, and host plants grow in within these planting substrates.

8. according to claim 1, a kind of method for mass production of Phytoseiid militaris is characterized in that said collection device is placed in a suitable space and refers to the space that is planned to be used for Phytoseiid militaris collection, and the space is required to be in When collecting Phytoseiid mites, it can be collected with or without light, but light is required when fixing the collection device, checking and replacing the collection tube, and cleaning the plant stems, branches or leaves in the collection device.

9. according to claim 1, a kind of method for mass production of Phytoseiid militaris is characterized in that said collection container refers to the part for filling Phytoseiid militaris and plant stem, branch or leaf, and the collection container has The volume of 0.001m ³ -1m ³ , the manufacturing material of the collection container must contain gauze, cloth, insect-proof net, paper or other materials that can breathe freely and can effectively prevent Phytoseius chileensis from escaping. The gauze, cloth, insect-proof Net, paper or other materials that are breathable and can effectively prevent phytoseioids from escaping are used alone or in combination of two or more. It cannot climb out from other places outside the collection tube. The collection container also has an opening that is easy to open and seal. Open the opening to fill Phytoseiid mites and plant stems, branches or leaves, and seal the opening to seal the collection container. Wherein the opening on the collecting container for filling the phytoseiid mites and plant stems, branches and leaves is larger than the opening through which the phytoseiid mites crawl out from the collection container.

10. according to claim 1, a kind of method for mass production of Phytoseiillus paliscens, is characterized in that said collection conduit refers to the passage that is installed on the collection container for Phytoseius paliscens to climb out, Mites can crawl out of the collection container from the end where the collection conduit is installed on the collection container, and then crawl into the collection pipe from the end where the collection conduit and the collection pipe are connected together. The number of collection conduits on the collection container is one or more. The length of the collecting conduit is within 1m.