CN104188857B - Traditional Chinese medicine skin-whitening fleck-banishing cream and preparation method thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine whitening cream and a preparation method thereof. The whitening cream contains whitening components such as angelica extract, licorice extract, white peony extract, white pomegranate extract, atractylodes macrocephala extract, and polygonatum polygonatum extract extract and mulberry leaf extract, and also contains oil phase matrix components and water phase matrix components. The preparation method is to mix the oil-phase base component and the water-phase base component separately at high temperature, add the oil-phase base component into the water-phase base component, stir evenly, and then add the traditional Chinese medicine whitening component into it, After vacuum homogeneous emulsification, cool down, add essence and mix well. The traditional Chinese medicine whitening and spot-lightening cream prepared by the invention has a strong whitening and spot-lightening effect, and is safe and non-irritating.

Description

A kind of traditional Chinese medicine whitening cream and preparation method thereof

technical field

The invention belongs to the technical field of cosmetics, and relates to a whitening cream and a preparation method thereof, in particular to a cream with traditional Chinese medicine extracts as whitening ingredients.

Background technique

As the saying goes: one white covers one hundred ugliness, fair skin has always been the pursuit of many beauty lovers, especially for Asian women with natural yellow skin, having fair skin has always been an important issue in beauty. In recent years, with the continuous improvement of people's living standards, people's requirements for beauty are getting higher and higher, and the market share of whitening and spot-lightening cosmetics has always been in a rapid growth trend.

The whitening active ingredients used in whitening cosmetics in the early days include mercury compounds, hormones and hydroquinone, etc. These compounds generally act quickly and have obvious whitening effects, but they are highly toxic and easy to deposit in the body, causing internal organ failure or damaging the skin. It is highly irritating and can easily lead to cell lesions. It has been banned by countries all over the world. At present, the skin whitening agent added in cosmetics should meet two standards: one is that it has a high inhibition rate on tyrosinase activity, which can significantly reduce the production of melanin; the other is that it has high safety and is harmless to human skin. Toxic and non-irritating. It can be seen that preparing a non-toxic and non-irritating whitening cream with good whitening effect will be favored by people.

Contents of the invention

The object of the present invention is to provide a traditional Chinese medicine whitening cream.

Another object of the present invention is to provide a preparation method of traditional Chinese medicine whitening cream.

The technical scheme that the present invention takes is:

A traditional Chinese medicine whitening cream, which contains angelica extract, licorice extract, white peony extract, white pomegranate extract, Atractylodes macrocephala extract, Polygonatum odoratum extract and mulberry leaf extract.

Further, the above-mentioned traditional Chinese medicine whitening cream consists of 0.5-5.0 parts of traditional Chinese medicine whitening components, 8.8-22.65 parts of oil phase base components and 72.35-90.7 parts of water phase base components in parts by mass; wherein, The traditional Chinese medicine whitening component is composed of the following raw material proportions by mass: 1.6-2.4 parts of angelica extract, 1.6-2.4 parts of licorice extract, 1.6-2.4 parts of white peony extract, 0.8-1.2 parts of 0.8 to 1.2 parts of fruit, 0.8 to 1.2 parts of Polygonatum odoratum extract, and 0.8 to 1.2 parts of mulberry leaf extract.

Further, the above-mentioned oil phase base component is composed of the following raw material proportions in parts by mass: 0-3.0 parts of stearic acid, 0-2.0 parts of beeswax, 1.0-3.0 parts of white oil, 1.0-4.0 parts of cetyl alcohol, and -60 0.2-0.5 parts, 0.5-2.0 parts of glyceryl monostearate, 0.5-1.5 parts of olive oil, 0.5-1.5 parts of dimethyl silicone oil, 0.05-0.15 parts of methylparaben, 0.05 parts of propylparaben ~0.15 parts.

Further, the above-mentioned aqueous matrix is composed of the following raw material proportions by mass: 0.5-1.5 parts of glycerin, 0.5-1.0 parts of propylene glycol, 0.5-2.0 parts of water-soluble jojoba oil, 0.5-2.0 parts of hyaluronic acid, Tween- 60 0.5-2.0 parts, Carbopol 940 0-0.5 parts, triethanolamine 0.1-0.5 parts, essence 0.05-0.2 parts, water 60.0-83.8 parts.

A preparation method of traditional Chinese medicine whitening cream, comprising the following steps:

1) Weigh each raw material of the whitening component of the traditional Chinese medicine according to the raw material ratio of the whitening component of the traditional Chinese medicine, and mix them evenly;

2) Weigh each raw material of the oil phase base component according to the ratio of the raw materials of the oil phase base component, add it into the oil phase pot, heat to 80-90°C, and stir evenly;

3) Weigh each raw material of the water-phase matrix component according to the raw material ratio of the above-mentioned water-phase matrix component, add all other raw materials except essence into the water-phase pot, heat to 80-90°C, and stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue stirring for 5-10 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is (0.5-5): (8.8-22.65): (72.35-90.7).

The beneficial effects of the present invention are:

1) The whitening components of traditional Chinese medicine in the present invention are selected by using the tyrosinase activity inhibition method to detect a variety of Chinese medicine extracts with whitening effects, and screen out angelica extract, licorice extract, white peony extract, and white peony extract. It is prepared by mixing the extracts of Potassium officinalis, Atractylodes macrocephala, Polygonatum odoratum and Mulberry leaves according to the mass ratio of 2:2:2:1:1:1:1. At present, the above seven kinds of drugs have not been used in combination in whitening and spot-lightening products, and the traditional Chinese medicine whitening spot-lightening cream prepared by the present invention has a strong whitening and spot-lightening effect, is safe and non-irritating.

2) The present invention provides a safe and efficient whitening cream prepared from various traditional Chinese medicine extracts, which can moisturize and moisturize the skin in addition to whitening and lightening the spots. It uses traditional Chinese medicine extracts as whitening product additives, because of its low toxic and side effects, high safety, cost-effectiveness, and also conforms to the current development trend of returning to nature and people's consumption psychology, and has great development potential.

Description of drawings

Fig. 1 is the influence of different samples on the content of melanin between guinea pig skin dermis and epidermis;

Fig. 2 is the influence of different samples on melanin content in guinea pig hair follicles and hair;

Figure 3 shows the change of pigmented melanin after using different whitening products;

Fig. 4 is the change of the color of the nevus and its surrounding skin after using the traditional Chinese medicine whitening cream prepared in Example 2.

detailed description

The present invention is based on the long-term research on the mechanism of skin whitening by traditional Chinese medicine, referring to modern pharmacological research results, and using biochemical enzyme method (tyrosinase activity inhibition method) to screen out 7 kinds of Chinese medicine extracts for whitening and lightening spots, and Compatibility and combination make traditional Chinese medicine whitening cream.

The possible mechanism of action of the traditional Chinese medicine whitening cream of the present invention

In traditional Chinese medicine, in addition to promoting blood circulation and promoting muscle growth, antibacterial and anti-inflammatory, and regulating the immune function of the body, angelica also has the effect of removing spots and whitening. This is mainly due to the substances with 2-hydroxy-4 methoxyphenyl structure and Phenolic substances have an inhibitory effect on tyrosinase. Licorice is a commonly used traditional Chinese medicine. Studies have proved that its extract is a very good whitening raw material, which has multiple functions such as anti-inflammatory, moisturizing, scavenging oxygen free radicals, and good UV protection. Radix Paeoniae Alba, Bai Po, and Atractylodes macrocephala are all traditional medicines for moisturizing the skin, whitening and lightening spots. They are used in many skin-beautifying prescriptions such as Qibai San and Yurong San in ancient times. Theories of traditional Chinese medicine believe that these medicines can treat sallow complexion, Face spots, dull. "Shen Nong's Materia Medica" says: Yuzhu "long-term use to remove dark face, good color, moist, light body and not old", which shows that Yuzhu has the effect of beautifying and prolonging life. Modern research shows that Polygonatum Polygonatum contains glucoside, kaempferol glycoside, quercetin glycoside, provitamin A, mucilage and other ingredients. Provitamin A can be converted into vitamin A after entering the body, which can help improve chapped and rough skin and make the skin soft, while the mucus can make the skin smooth. According to traditional Chinese medicine, mulberry leaves are bitter, sweet and cold in nature and have the effects of dispersing wind and heat, clearing liver and improving eyesight. Studies in recent years have proved that mulberry leaves also have good skin beautification effects, especially for acne and brown spots on the face.

In addition to inhibiting the synthesis of melanin, the traditional Chinese medicine whitening cream of the present invention may also promote the shedding of keratinocytes containing melanin, or limit the ability of melanin cells to produce melanin, thereby achieving the effect of whitening.

The present invention will be further described below in conjunction with specific examples, but is not limited thereto.

Example 1

1) Weigh and mix the raw materials that make up the traditional Chinese medicine whitening component according to the following proportions by mass:

2.0 parts of angelica extract, 2.0 parts of licorice extract, 2.0 parts of white peony extract, 1.0 part of white pomegranate extract, 1.0 part of Atractylodes macrocephala extract, 1.0 part of Polygonatum odoratum extract, 1.0 part of mulberry leaf extract;

2) Weigh the raw materials that make up the oil phase matrix components according to the following proportions by mass: 0.5 parts of beeswax, 3.0 parts of white oil, 1.5 parts of cetyl alcohol, 0.5 parts of Span-60, and 1.0 parts of glyceryl monostearate , 0.5 parts of olive oil, 2.0 parts of simethicone oil, 0.1 part of methylparaben, and 0.1 part of propylparaben; put all the raw materials into the oil phase pot, heat to 80-90°C, and stir evenly;

3) Weigh the raw materials constituting the aqueous phase matrix components according to the following mass ratio: 1.5 parts of glycerin, 0.5 parts of propylene glycol, 2.0 parts of water-soluble jojoba oil, 0.5 parts of hyaluronic acid, 1.0 parts of Tween-60, 0.2 parts of Bo 940, 0.2 parts of triethanolamine, 0.1 parts of essence, and 83.8 parts of distilled water; add all other raw materials except essence into the water phase pot, heat to 80-90°C, and stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue to stir for 5 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is 1:9.2:89.8.

Example 2

1) Weigh and mix the raw materials that make up the traditional Chinese medicine whitening component according to the following proportions by mass:

2.0 parts of angelica extract, 2.0 parts of licorice extract, 2.0 parts of white peony extract, 1.0 part of white pomegranate extract, 1.0 part of Atractylodes macrocephala extract, 1.0 part of Polygonatum odoratum extract, 1.0 part of mulberry leaf extract;

2) Weigh the raw materials that make up the oil phase matrix components according to the following mass ratio: 1.0 parts of beeswax, 3.0 parts of white oil, 1.8 parts of cetyl alcohol, 1.5 parts of Span-60, 2.0 parts of glyceryl monostearate , 1.0 parts of olive oil, 1.0 parts of simethicone oil, 0.15 parts of methylparaben, and 0.15 parts of propylparaben; put each raw material into the oil phase pot, heat to 80-90°C, and stir evenly;

3) Weigh the raw materials constituting the aqueous phase matrix components according to the following mass ratio: 2.0 parts of glycerin, 1.0 parts of propylene glycol, 1.0 parts of water-soluble jojoba oil, 1.0 parts of hyaluronic acid, 2.0 parts of Tween-60, 0.3 parts of Bo 940, 0.3 parts of triethanolamine, 0.1 parts of essence, and 77.7 parts of distilled water; add all other raw materials except essence into the water phase pot, heat to 80-90°C, and stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue to stir for 5 minutes, then add the Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is 3:11.6:85.4.

Example 3

1) Weigh and mix the raw materials that make up the traditional Chinese medicine whitening component according to the following proportions by mass:

2.0 parts of angelica extract, 2.0 parts of licorice extract, 2.0 parts of white peony extract, 1.0 part of white pomegranate extract, 1.0 part of Atractylodes macrocephala extract, 1.0 part of Polygonatum odoratum extract, 1.0 part of mulberry leaf extract;

2) Weigh the raw materials that make up the oil phase matrix components according to the following proportions by mass: 3.0 parts of stearic acid, 2.0 parts of white oil, 3.0 parts of cetyl alcohol, 1.0 parts of Span-60, glyceryl monostearate 1.0 parts, olive oil 1.0 parts, simethicone oil 1.5 parts, methylparaben 0.1 part, propylparaben 0.1 part; put each raw material into the oil phase pot, heat to 80～90℃, stir well ;

3) Weigh the raw materials constituting the aqueous phase matrix components according to the following mass ratio: 1.5 parts of glycerin, 1.5 parts of propylene glycol, 1.0 parts of water-soluble jojoba oil, 1.0 parts of hyaluronic acid, 1.0 parts of Tween-60, three 0.15 parts of ethanolamine, 0.1 part of essence, 79.05 parts of distilled water; add all other raw materials except essence into the water phase pot, heat to 80-90°C, and stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue to stir for 5 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is 2:12.7:85.3.

Example 4

1) Weigh and mix the raw materials that make up the traditional Chinese medicine whitening component according to the following proportions by mass:

2.0 parts of angelica extract, 2.0 parts of licorice extract, 2.0 parts of white peony extract, 1.0 part of white pomegranate extract, 1.0 part of Atractylodes macrocephala extract, 1.0 part of Polygonatum odoratum extract, 1.0 part of mulberry leaf extract;

2) Weigh the raw materials that make up the oil phase matrix components according to the following proportions by mass: 4.0 parts of stearic acid, 2.0 parts of white oil, 3.0 parts of cetyl alcohol, 1.0 parts of Span-60, glyceryl monostearate 1.0 parts, olive oil 1.0 parts, simethicone oil 2.0 parts, methylparaben 0.15 parts, propylparaben 0.15 parts; put each raw material into the oil phase pot, heat to 80～90℃, stir well ;

3) Weigh the raw materials constituting the aqueous phase matrix components according to the following mass ratio: 1.0 parts of glycerin, 1.0 parts of propylene glycol, 1.5 parts of water-soluble jojoba oil, 0.5 parts of hyaluronic acid, 1.0 parts of Tween-60, three 0.15 parts of ethanolamine, 0.1 part of essence, 76.45 parts of distilled water; add all other raw materials except essence into the water phase pot, heat to 80-90°C, and stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue to stir for 5 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is 4:14.3:81.7.

Example 5

1) Weigh and mix the raw materials that make up the traditional Chinese medicine whitening component according to the following proportions by mass:

2.0 parts of angelica extract, 2.0 parts of licorice extract, 2.0 parts of white peony extract, 1.0 part of white pomegranate extract, 1.0 part of Atractylodes macrocephala extract, 1.0 part of Polygonatum odoratum extract, 1.0 part of mulberry leaf extract;

2) Weigh the raw materials constituting the oil phase matrix components according to the following mass ratio: 1.0 parts of stearic acid, 0.5 parts of beeswax, 1.0 parts of white oil, 1.5 parts of cetyl alcohol, 1.0 parts of Span-60, single hard 1.0 parts of fatty acid glyceride, 0.5 parts of olive oil, 2.0 parts of simethicone oil, 0.15 parts of methyl paraben, and 0.15 parts of propyl paraben; put all the raw materials into the oil phase pot and heat to 80-90 ℃, stir evenly;

3) Weigh the raw materials constituting the aqueous phase matrix components according to the following mass ratio: 1.0 parts of glycerin, 0.5 parts of propylene glycol, 3.0 parts of water-soluble jojoba oil, 0.5 parts of hyaluronic acid, 2.0 parts of Tween-60, cal 0.2 parts of Bo 940, 0.3 parts of triethanolamine, 0.1 parts of essence, and 80.6 parts of distilled water; add other raw materials except essence to the water phase pot, heat to 80-90°C, and stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue to stir for 5 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is 3:8.8:88.2.

Example 6

1) Weigh and mix the raw materials that make up the traditional Chinese medicine whitening component according to the following proportions by mass:

2.0 parts of angelica extract, 2.0 parts of licorice extract, 2.0 parts of white peony extract, 1.0 part of white pomegranate extract, 1.0 part of Atractylodes macrocephala extract, 1.0 part of Polygonatum odoratum extract, 1.0 part of mulberry leaf extract;

2) Weigh the raw materials that make up the oil phase matrix components according to the following mass ratio: 3.0 parts of stearic acid, 1.0 parts of white oil, 2.0 parts of cetyl alcohol, 1.0 parts of Span-60, glyceryl monostearate 0.75 parts, 0.5 parts of olive oil, 1.0 parts of simethicone oil, 0.15 parts of methylparaben, 0.15 parts of propylparaben; put all the raw materials into the oil phase pot, heat to 80-90℃, and stir well ;

3) Weigh the raw materials constituting the aqueous phase matrix components according to the following mass ratio: 0.5 parts of glycerin, 0.5 parts of propylene glycol, 2.0 parts of water-soluble jojoba oil, 0.5 parts of hyaluronic acid, 1.0 parts of Tween-60, 0.2 parts of Bo 940, 0.3 parts of triethanolamine, 0.1 parts of essence, and 80.35 parts of distilled water; add other raw materials except essence to the water phase pot, heat to 80-90°C, and stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue to stir for 5 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is 5:9.55:85.45.

Example 7

1) Weigh and mix the raw materials that make up the traditional Chinese medicine whitening component according to the following proportions by mass:

2.0 parts of angelica extract, 2.0 parts of licorice extract, 2.0 parts of white peony extract, 1.0 part of white pomegranate extract, 1.0 part of Atractylodes macrocephala extract, 1.0 part of Polygonatum odoratum extract, 1.0 part of mulberry leaf extract;

2) Weigh the raw materials that make up the oil phase matrix components according to the following mass ratio: 6.0 parts of stearic acid, 1.0 parts of white oil, 6.0 parts of cetyl alcohol, 1.0 parts of Span-60, glyceryl monostearate 4.85 parts, 0.5 parts of olive oil, 3.0 parts of simethicone oil, 0.15 parts of methylparaben, 0.15 parts of propylparaben; put all the raw materials into the oil phase pot, heat to 80-90℃, and stir well ;

3) Weigh the raw materials constituting the aqueous matrix component according to the following mass ratio: 5.0 parts of glycerin, 3.5 parts of propylene glycol, 2.0 parts of water-soluble jojoba oil, 0.5 parts of hyaluronic acid, 1.0 parts of Tween-60, 0.2 parts of wave 940, 0.2 parts of triethanolamine, 0.1 parts of essence, 59.85 parts of distilled water (the sum of parts by mass here does not necessarily have to be 72.35); ℃, stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue to stir for 5 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is 5:22.65:72.35.

Example 8

1) Weigh and mix the raw materials that make up the traditional Chinese medicine whitening component according to the following proportions by mass:

2.0 parts of angelica extract, 2.0 parts of licorice extract, 2.0 parts of white peony extract, 1.0 part of white pomegranate extract, 1.0 part of Atractylodes macrocephala extract, 1.0 part of Polygonatum odoratum extract, 1.0 part of mulberry leaf extract;

2) Weigh the raw materials that make up the oil phase matrix components according to the following proportions by mass: 5.0 parts of stearic acid, 1.0 parts of white oil, 5.0 parts of cetyl alcohol, 1.0 parts of Span-60, glyceryl monostearate 3.75 parts, 0.5 parts of olive oil, 1.0 parts of simethicone oil, 0.15 parts of methylparaben, 0.15 parts of propylparaben; put all the raw materials into the oil phase pot, heat to 80～90℃, stir well ;

3) Weigh the raw materials that make up the aqueous phase matrix components according to the following mass ratio: 3.5 parts of glycerin, 3.5 parts of propylene glycol, 2.0 parts of water-soluble jojoba oil, 0.5 parts of hyaluronic acid, 1.0 parts of Tween-60, 0.2 parts of Bo 940, 0.3 parts of triethanolamine, 0.1 parts of essence, 70.85 parts of distilled water; add all other raw materials except essence into the water phase pot, heat to 80-90°C, and stir evenly;

4) Under stirring, slowly add the oil phase base components into the water phase base components, continue to stir for 5 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly;

5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is 0.5:17.55:81.95.

The following is a further effect test on the traditional Chinese medicine whitening and lightening cream prepared in the above examples.

The present invention is based on the long-term research on the mechanism of skin whitening by traditional Chinese medicine, and with reference to the results of modern pharmacological research, 7 kinds of Chinese medicine extracts for whitening and blemish are screened out by biochemical enzyme method, and the Chinese medicine whitening and blemish cream is prepared by combination , It has been verified by animal experiments and human trials that it is safe and non-irritating, and has good whitening and blemish effects.

1. Whitening effect of guinea pigs

Take 10 black guinea pigs, male and female, weighing 250-350 g, and divide them into 5 groups, 4 in each group, respectively the low-dose Chinese medicine whitening cream group (1%) in Example 1 and the medium-dose Chinese medicine whitening cream group in Example 2. (3%), the high-dose Chinese medicine whitening cream group of Example 6 (5%), the positive arbutin whitening cream group and the blank group. The treatment group shaved the hair on both sides of the back to form a 3cm×3cm depilated area, took the experimental reagent with a cotton swab and applied it evenly on the left and right sides of the skin, twice a day. Animals in the blank group were also depilated on both sides, and other experimental conditions were guaranteed to be the same as those in the experimental group to form a control.

During the feeding process of guinea pigs, the indoor temperature should be strictly controlled at around 25°C, and the relative humidity should be kept between 50% and 60%. Each guinea pig eats 10-15g of feed, 10-15g of carrots or vegetables, and unlimited water supply. .

After 28 days, skin biopsies from guinea pigs were taken, fixed with 10% formalin, embedded in paraffin, sectioned, and observed. Microscopically photograph the sections stained with ferrous sulfate (as shown in Figure 1 and Figure 2), and then use Image-Pro Plus6.0 software to analyze the photographs, using optical density/section area and optical density/melanocyte area To characterize the melanin content. 40 photos were randomly taken for each slide for statistical analysis. The results are as follows:

Table 1 Comparison of melanin values in different groups after staining by ferrous sulfate absorption method

Note: * means P<0.05, ** means P<0.01, *** means P<0.001, n=40.

As can be seen from the above results, whether it is analyzed from the perspective of optical density/section area or optical density/melanocyte area, there are obvious differences between the blank group and the experimental group. The difference is not big, which also shows that the traditional Chinese medicine whitening cream will not have sex-selective effect. The results of the positive arbutin group, the middle dose group (Example 2), and the high dose group (Example 6) of the traditional Chinese medicine whitening cream are different but not far apart; at the same time, the effect strength is higher than that of the low dose (Example 1) , from this result, it can be shown that the whitening effect of this Chinese medicine whitening cream is related to the content of Chinese medicine extracts. From the prepared glass slides (as shown in Figure 1 and Figure 2), the collagen fibers are red, the muscle fibers are yellow, and the melanin is green to dark green. The melanin is mainly distributed at the junction of the dermis and epidermis and inside the hair. From Figure 2, it can be seen that the melanin content in the hair follicles and hairs of the low-dose group and the blank group is relatively high, and the distribution is relatively concentrated; compared with the three doses of low, medium and high doses, it can be seen that the melanin content decreases in turn, and the medium The pigment in the center of the hair root in the low-dose and high-dose groups became significantly thinner, and the pigment content in the epidermis was also lower. The results show that the traditional Chinese medicine whitening cream has a good whitening effect.

2. Human trial experiment

Select 4 volunteers with pigmentation, healthy skin, no photosensitivity, no cosmetic allergy history, no drug allergy history. Before the experiment started, the CBS-902 skin tester was used to detect the pigmented melanin, and the initial data could be obtained as an absolute reference value. After cleansing the skin every morning and evening, add the traditional Chinese medicine whitening cream prepared in Example 2, the commercially available whitening product 1 (Natural Tang Snow Whitening Cream), and the commercially available whitening product 2 (Avène Clear Whitening Cream) at a concentration of about 0.025 g/cm The amount of ² is evenly applied to the test site for 8 consecutive weeks. After the experiment started, the CBS-902 skin tester was used to detect the change of the melanin content of the spots every two days, and a curve was drawn according to the change. The change of skin pigment content of volunteers after 8 weeks of experiment is shown in Figure 3 and Figure 4.

It can be seen from Figure 3 that the three products have whitening effects on skin pigmentation. After 8 weeks of use, the pigmentation of product 1 decreased by 17%, that of product 2 decreased by 14%, and that of traditional Chinese medicine whitening cream decreased by 20%. The onset time is different, and the traditional Chinese medicine whitening and spot-lightening cream of the present invention has a relatively late onset time, and there is a relatively obvious change after one month of use. After 8 weeks of analysis, the traditional Chinese medicine whitening cream has a significant effect on pigmentation, and the skin color in the test area has decreased significantly (as shown in Figure 4). In addition to inhibiting the synthesis of melanin, the traditional Chinese medicine whitening cream of the present invention may also promote the shedding of keratinocytes containing melanin, or limit the ability of melanin cells to produce melanin, thereby achieving the effect of whitening. At the same time, it also shows that for whitening cosmetics, most qualified products can achieve whitening effect, but consumers are required to adopt appropriate methods during use and insist on using them. If you stop using it, it will be difficult to achieve the whitening effect.

Claims (4)

1. A traditional Chinese medicine whitening cream, characterized in that: in parts by mass, it consists of 0.5 to 5.0 parts of Chinese medicine whitening components, 8.8 to 22.65 parts of oil phase matrix components and 72.35 to 90.7 parts of water phase matrix components composition; Among them, the traditional Chinese medicine whitening component is composed of the following raw material proportions by mass: 1.6-2.4 parts of angelica extract, 1.6-2.4 parts of licorice extract, 1.6-2.4 parts of white peony extract, 0.8-1.2 parts of white pomegranate extract, 0.8-1.2 parts of Atractylodes macrocephala extract, 0.8-1.2 parts of Polygonatum odoratum extract, and 0.8-1.2 parts of mulberry leaf extract. 2. A traditional Chinese medicine whitening cream according to claim 1, characterized in that: the oil phase base component is composed of the following raw material ratios in parts by mass: 0-3.0 parts of stearic acid, 0-2.0 parts of beeswax 1.0-3.0 parts of white oil, 1.0-4.0 parts of cetyl alcohol, 0.2-0.5 parts of Span-60, 0.5-2 parts of glyceryl monostearate, 0.5-1.5 parts of olive oil, 0.5-0.5 parts of simethicone 1.5 parts, 0.05-0.15 parts of methylparaben, 0.05-0.15 parts of propylparaben. 3. A traditional Chinese medicine whitening and lightening cream according to claim 1, characterized in that: the water phase matrix is composed of the following raw material ratios in parts by mass: 0.5-1.5 parts of glycerin, 0.5-1.0 parts of propylene glycol, water-soluble 0.5-2.0 parts of jojoba oil, 0.5-2.0 parts of hyaluronic acid, 0.5-2.0 parts of Tween-60, 0-0.5 parts of Carbopol 940, 0.1-0.5 parts of triethanolamine, 0.05-0.2 parts of essence, 60.0-83.8 parts of water share. 4. The preparation method of a kind of traditional Chinese medicine whitening cream according to any one of claims 1 to 3, characterized in that: comprising the following steps: 1) Weigh the raw materials of the traditional Chinese medicine whitening component according to the raw material ratio described in claim 1, and mix them evenly; 2) Weigh the raw materials of the oil-phase matrix component according to the raw material ratio described in claim 2, add them into the oil-phase pot, heat to 80-90°C, and stir evenly; 3) Weigh each raw material of the water-phase matrix component according to the raw material ratio described in claim 3, add other raw materials except essence into the water-phase pot, heat to 80-90°C, and stir evenly; 4) Under stirring, slowly add the oil phase base components into the water phase base components, continue stirring for 5-10 minutes, then add the traditional Chinese medicine whitening components into the base, and stir evenly; 5) Emulsify the above mixture in a vacuum for 5-10 minutes; cool to 40-50°C, add essence, stir evenly, and cool to room temperature to obtain Chinese medicine whitening cream; wherein, Chinese medicine whitening component, oil phase base group The mass ratio of the component and the aqueous matrix component is (0.5-5): (8.8-22.65): (72.35-90.7).