CN104187712B - A kind of preparation method of Chinese caterpillar fungus health powder - Google Patents
A kind of preparation method of Chinese caterpillar fungus health powder Download PDFInfo
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- CN104187712B CN104187712B CN201410379558.5A CN201410379558A CN104187712B CN 104187712 B CN104187712 B CN 104187712B CN 201410379558 A CN201410379558 A CN 201410379558A CN 104187712 B CN104187712 B CN 104187712B
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- Prior art keywords
- powder
- enzymolysis
- caterpillar fungus
- chinese caterpillar
- pleurotus nebrodensis
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- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a kind of preparation method of Chinese caterpillar fungus health powder, belong to edible mushroom field of deep.The preparation method of described Chinese caterpillar fungus health powder is: by hickory chick enzymolysis powder, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder, animal protein hydrolysis powder and pickled vegetable fermentation liquor freeze-dried powder, after mixing in proportion, microwave disinfection, packaging are Chinese caterpillar fungus health powder.Product of the present invention has mushroom fragrance to be given prominence to, and taste is suitable for, nutritious feature; Pass through enzymolysis processing, total amino acid content improves 20-37% than before enzymolysis, simultaneously because the good compatibility of the various nutritional labelings of enzymolysis generation makes product of the present invention not only have good seasoning function but also have good nutrition nourishing function, it is a kind of edible mushroom deep processed product collecting nutrition, nourishing, adjusting immunity of human body function.This product instant, can be used as common food flavoring or chafing dish bottom flavorings eats, also direct-edible.
Description
Technical field
The invention belongs to edible mushroom field of deep, particularly a kind of preparation method of Chinese caterpillar fungus health powder.
Background technology
Pleurotus nebrodensis is a kind of rare edible mushroom, and analyze according to St Foods Q Supervision & Testing Center, Pleurotus nebrodensis protein content is up to 14.7%; Containing 18 seed amino acids in protein, wherein 8 seed amino acids of needed by human all contain.Every gram of Pleurotus nebrodensis is containing polysaccharide 190 milligrams.In amino acid, lysine is 569 milligrams/100 grams, is 2.8 times of general flat mushroom; Leucine 790.2 milligrams/100 grams, is 2.6 times of general flat mushroom; Isoleucine is 470.1 milligrams/100 grams, is 2.5 times of general flat mushroom; Valine 674.6 milligrams/100 grams is general flat mushroom 2.2 times, and also than general flat mushroom height 1.9-2.7 doubly, especially glutamic acid and arginine content are high especially for other human body essential amino acid content; Surprisingly arginine, lysine, these are conducive to the nutritional factors of intelligence development, than being called that the asparagus content of intelligence mushroom is taller in the world.Pleurotus nebrodensis also containing a large amount of multivitamins, mineral element and mushroom polysaccharide, can strengthen the immunologic function of human body.Cholesterol in its unrighted acid and blood of human body is combined into cholesterol ester, reduces blood pressure, prevents arteriosclerotic effect.Often edible Pleurotus nebrodensis disappears the effect of disease dissipating blood stasis and clearing away heat removing toxic substances in addition.Therefore the nutritive value that Pleurotus nebrodensis is surprising and health care cause the very big concern of scientific circles.
Hickory chick is under the jurisdiction of Ascomycotina (Ascomycotina), discomycete, Pezizale, Morchellaceae, morchella, and hickory chick has many small ruts with its cap surface, gains the name like sheep tripe in outward appearance pole.It is a kind of wild famous and precious edible and medical fungi, includes the earliest in Li Shizhen (1518-1593 A.D.) Compendium of Material Medica.The traditional Chinese medical science is used as medicine with Morchella esculenta (L.) Pers sporophore.Hickory chick is foremost delicious edible mushroom in sac fungus, and hickory chick contains protein 22.1%, fat 3.8%, polysaccharide 16.3%; And the unrighted acid in fat is 5 to 3 with the ratio of saturated fatty acid, the linoleic acid useful to human body accounts for 56% of fatty acid total amount.The amino acid of needed by human is complete, and its content is 5 times of Hericium erinaceus.Element also containing needed by human such as abundant potassium, phosphorus, calcium, iron, magnesium, zinc and selenium.Wherein the content of potassium, phosphorus is 4 times and 2 times of Cordyceps sinensis respectively, and Zn content is 9 times of Cordyceps sinensis; Selenium then can change carcinogenic metabolism direction and anti-oxidant, and thus hickory chick is described as " anticancer star ".
Hickory chick property is put down, and taste is sweet cold, nontoxic, there is beneficial stomach, digestion help food, phlegm-eliminiating and qi-regulating, kidney tonifying, establishing-Yang, benefit brain, the function of refreshing oneself.Research finds that hickory chick has reducing blood lipid, immunity moderation, antifatigue, anti-radiation, antitumor action, and can alleviate the toxic and side effect that cancer patient's chemicotherapy causes." food and biotechnology journal " volume the 2nd March the 26th in the 2007th phase " Study on Liquid Fermentation Technology of Morchella esculenta " has been done to dissolve in research to hickory chick liquid deep layer fermenting, and the best medium proportioning of selection is: corn flour 4.0g/dL, glucose 1.0g/dL, analysis for soybean powder 2.0g/dL, dusty yeast 0.3g/dL, KH
2pO
40.2g/dL, MgSO
40.1g/dL, CaSO
40.1g/dL; Selection optimal culture conditions is: 24 DEG C, and the shaking flask liquid amount of initial pH5.8,250mL is 100mL, inoculum concentration 10mL, shaking flask rotating speed 140r/min, and fermentation time is 108h.
At present the research of Mushroom production, mushroom processing is come into one's own day by day, on market, the kind of mushroom converted products mainly concentrates on fresh goods, dried product and canned pack three aspects, publication number is the edible mushroom powder that the Chinese invention " mushroom powder and production technology thereof " of CN1136408A discloses in solid powdery, production technology is: fresh mushroom, rinsing, fragmentation, breaking-wall cell, separation, ultrafiltration, oven dry, and its technique is simple but nutrition and the reservation of delicate flavour composition are not fine.Publication number is that the Chinese invention " a kind of compound mushroom flavoring and preparation method thereof " of CN101617808A discloses the flavoring that a kind of primary raw material is agaricus bisporus powder, straw mushroom powder, sodium glutamate etc., wherein the preparation method of mushroom powder is: after fresh mushroom rinsing, boiling, pulverizing, defibrination, homogeneous, then obtains through concentrated, spraying dry.Also it is not high to there is raw material extraction and application rate in this method, and nutrition and delicate flavour retain bad problem.Publication number be CN101455332A Chinese invention " a kind of edible fungus composition of disease preventing and treating " disclose one can effective disease preventing and treating, the edible fungus composition taken good care of one's health, be made up of the raw material of following percentage by weight: auricularia auriculajudae 8-10%, straw mushroom 9-11%, mushroom 7-9%, Chinese caterpillar fungus 9-11%, asparagus 11-13%, white fungus 10-12%, Chinese caterpillar fungus 12-14%, flat mushroom 7-9%, hedgehog hydnum 8-10%, spice 2-3%, flavouring 2-3% and water 4-6%, total amount 100%.First by auricularia auriculajudae, straw mushroom, mushroom, Chinese caterpillar fungus, asparagus, white fungus, Chinese caterpillar fungus, flat mushroom and hedgehog hydnum cutting slivering or block during making, then add spice, flavouring and water, place in pressure cooker, high temperature 120-150 DEG C, boiling 5-10 minute, lets cool, tinning or bottling or pack.Publication number is the manufacture craft that the Chinese invention " a kind of anti-tumor healthcare mushroom porridge " of CN103494180A provides a kind of anti-tumor healthcare mushroom porridge, this mushroom porridge comprises Hericium erinaceus, northern hickory chick, asparagus, Chinese caterpillar fungus, white fungus, flat mushroom, pleurotus eryngii, seafood mushroom, crab flavour mushroom, tomato, the seed of Job's tears, rice etc., each raw material is allocated in proportion, selected ingredient requirement green high quality, without disease and pest.Preparation flow comprises screening and pretreatment, boiling, tinning, the sterilizing etc. of raw material, and manufacturing process does not add any anticorrisive agent and food additives.It is characterized by: to have the edible mushroom of antitumor efficacy for primary raw material, and raw material is fresh fruit body of edible fungi, arranging in pairs or groups with antitumor vegetables, cereal uses, and enhances value and the nutritive value of various raw material.This mushroom porridge comprises various active material as hickory chick element, Flammulin, cordycepin etc., and mushroom taste is strong, nutritious, and have significantly prevention and health-care effect to develop immunitypty, pre-preventing tumor, its technological operation is simple, suitable factorial praluction.Publication number is the preparation method that the Chinese invention " instant edible mushroom and preparation method thereof " of CN101238878A provides a kind of instant edible mushroom, be characterised in that the flavoring of use comprises mushroom powder, this mushroom powder comprises matsutake dry powder 9-11 by mass parts, Boletus luridus dry powder 18-22, flower mushroom dry powder 9-11, HEIHUZHANG fungus dry powder 9-11, hickory chick dry powder 9-11, Termitomyces albuminosus dry powder 9-11, Boletus aereus dry powder 90-110, the technological process of Hericium erinaceus dry powder 9-11. its preparation method is simple, with low cost; The instant product mouthfeel made is excellent, non-greasy, has the feature of the natural fresh perfume (or spice) of strong mushroom.Described mushroom powder is obtained, granularity 40-120 order lower than 12% rear grinding to water content by corresponding fresh feed bacterium natural air drying.
Pickles belong to the victual that fermentation " cold working " makes.Due to the uniqueness of processing technology and preparation method, very favourable to the nutritional labeling and color, smell and taste body keeping vegetables, make kimchi products not only fully remain the good organoleptic quality of original vegetables and nutritional labeling, and give its functional characteristic upgraded.
The nutritional labeling of pickles is very abundant, except containing except the nutritional labelings such as protein, dietary fiber, calcium, phosphorus, iron, carrotene, capsicim, also containing vitamin A, B
1, B
2, the nutritional labeling such as C.Meanwhile, due to the fermentation of beneficial bacterium, the metabolite of generation gives again pickles certain functional.For this reason, pickles become unfailing product for thousands of years among the people.
Kimchi products in fermenting and producing due to the effect of salt and fermentation, the moisture of vegetables itself can leach in a large number, produce a large amount of pickle fermentation liquid, these pickled vegetable fermentation liquors be rich in vitamin ingredients in lactic acid bacteria in pickle fermentation, lactic acid, vegetables, mineral matter that other vegetables contain.Pickled vegetable fermentation liquor generally directly discharges by current pickle production producer, causes the waste of the zymotic fluid being rich in nutritional labeling in a large number, and causes environmental pollution in various degree.As can be seen here, scientific utilization pickled vegetable fermentation liquor not only can make a large amount of utility be utilized, on the other hand also can protection of the environment, decreasing pollution.
Chinese patent: " preparation methods of bioanalysis Rapid Fermentation pickles ", the patent No. is 201110421967.3; Disclose the preparation method of bioanalysis Rapid Fermentation pickles, belong to field of vegetable deep-processing, particularly utilize composite bacterium powder to produce the method for pickles.This invention addresses the technical problem of quick production high-quality fermentation pickled vegetable, key step has: (1) reinforced sealing: put into container after vegetable raw-material cutting, add the composite bacterium powder containing acetobacter, S. cervisiae and the auxiliary material containing salt, airtight container; (2) ferment: control temperature carries out prior fermentation at 23-36 DEG C, temperature is controlled subsequently to carry out after fermentation at 15-25 DEG C, and whole fermentation time is at 20-40 hour; (3) dehydration allotment: ferment and completely slough pickles moisture, add flavoring for mixture evenly.This invention is applicable to the pickle production of different scales, obviously can shorten the production time, kimchi products is nutritious, pure taste, and this product has broad application prospects in pickle production field.
Chinese patent " preparation methods of the prebiotic pickles of bioanalysis Rapid Fermentation ", application number: 201210310308.7; This disclosure of the invention preparation method of the prebiotic pickles of bioanalysis Rapid Fermentation, belongs to field of vegetable deep-processing, particularly utilizes composite bacterium powder to produce the method for pickles.This invention addresses the technical problem of quick production high-quality fermentation pickled vegetable, key step has: (1) reinforced sealing: put into container after vegetable raw-material cutting, add the composite bacterium powder containing acetobacter, S. cervisiae and the auxiliary material containing salt, airtight container; (2) ferment: control temperature carries out prior fermentation at 23-36 DEG C, temperature is controlled subsequently to carry out after fermentation at 15-25 DEG C, and whole fermentation time is at 20-40 hour; (3) dehydration allotment: ferment and completely slough pickles moisture, add flavoring for mixture evenly.This invention is applicable to the pickle production of different scales, obviously can shorten the production time, kimchi products is nutritious, pure taste, and this product has broad application prospects in pickle production field.
How to make full use of mushroom nutritious, delicious in taste, be rich in the feature of various nutritional labeling, improve raw material extraction, utilization rate, exploitation has the flavoring products of good nutrition health care, meet and enrich the growing material requisite of people, developing edible mushroom new application is edible mushroom field of deep problem demanding prompt solution.
Summary of the invention
The technical problem that the present invention solves is to provide a kind of preparation method of Chinese caterpillar fungus health powder.
A preparation method for Chinese caterpillar fungus health powder, comprises the steps: that, by hickory chick enzymolysis powder, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder, animal protein hydrolysis powder and pickled vegetable fermentation liquor freeze-dried powder, after mixing in proportion, microwave disinfection, packaging are Chinese caterpillar fungus health powder.
The parts by weight of described Chinese caterpillar fungus health powder consist of: hickory chick enzymolysis powder 10-25, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder 25-35, animal protein hydrolysis powder 2-5, pickled vegetable fermentation liquor freeze-dried powder 2-6.
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 40-60 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 0.5-2%, temperature 30-50 DEG C, pH4-5, time 0.5-1 hour; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.3-1%, hydrolysis temperature 45-50 DEG C, pH value 6-7, after enzymolysis time 1-2 hour, adjustment temperature keeps 5-10 minute to 90-110 DEG C, cooling; Enzymolysis liquid drying obtains hickory chick enzymolysis powder.
Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder preparation process is as follows: Chinese caterpillar fungus, after Pleurotus nebrodensis sporophore and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:3-6 with water, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, adjust temperature after adding Chinese caterpillar fungus and Pleurotus nebrodensis sporophore quality 0.1-0.3% cellulase degradation 1-1.5 hour and keep 2-5 minute to 80-95 DEG C, adjustment temperature is to 50 DEG C, interpolation Chinese caterpillar fungus and Pleurotus nebrodensis sporophore quality 0.1-0.3% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 40-50 DEG C, pH value 6-7, enzymolysis time 1-2 hour, centrifugation obtains supernatant, supernatant spraying dry obtains Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder.Described Chinese caterpillar fungus, Pleurotus nebrodensis sporophore and Radix Isatidis are all crushed to particle diameter at below 0.5mm.The parts by weight of Chinese caterpillar fungus, Pleurotus nebrodensis and Radix Isatidis are respectively Chinese caterpillar fungus 10-15, Pleurotus nebrodensis 5-15, Radix Isatidis 0.1-0.2;
Animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:5.1-6 and through milling treatment of colloid, adjustment colloid mill stator and the gap of rotor are 105-120 micron, colloid mill flow-control be 0.2-0.5 ton/hour, it is 40-60 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 5.0-7.0, add the papain enzymolysis 60-100 minute of animal flesh quality 0.12-0.25%, 5-10 DEG C is cooled to keep 1.5-2 hour, increase the temperature to 85-95 DEG C and keep 10-25 minute, control temperature is to 40-50 DEG C, add animal flesh quality 0.12-0.25% flavor protease and carry out enzymolysis, hydrolysis temperature 40-50 DEG C, pH value 6-7, enzymolysis time 1-2 hour, obtain animal protein factor digest, get centrifuged supernatant (centrifugal rotational speed 3000-5000rpm) spraying dry and obtain animal protein hydrolysis powder.
Described animal flesh is chicken;
The enzyme preparation of adding in the present invention is purchased from Novi's letter, Beijing Dong Huadeng enzyme preparation company.
The root of bidentate achyranthes and the bark of eucommia enzymolysis liquid of 10-20% is added in fermentation medium in toadstool ferment process;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 orders, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, keeps 30-60 minute, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
Product preparation process of the present invention is as follows:
Take each raw material by formula, mix rear microwave disinfection, packaging is product.
Beneficial effect
This product has mushroom fragrance to be given prominence to, and taste is suitable for, nutritious feature, both can make flavoring, can help food separately again, is the good merchantable brand of tourism at home.Pass through enzymolysis processing, protein in its fructification tissue is made to obtain degraded to a certain extent, amino acid analyzer is utilized to measure, total amino acid content improves 20-37% than before enzymolysis, effectively raise the content of each seed amino acid and other flavor substances in product of the present invention, make product of the present invention delicious in taste, simultaneously because the good compatibility of the various nutritional labelings of enzymolysis generation makes product of the present invention not only have good seasoning function but also have good nutrition nourishing function.Polysaccharide in final hickory chick enzymolysis powder is made to improve more than 10% after adding the root of bidentate achyranthes of 10-20% and bark of eucommia enzymolysis liquid in toadstool ferment culture medium.
The preparation of animal protein hydrolysis powder, high-pressure homogeneous, enzymolysis high-temperature process in mid-term subsection enzymolysis treatment process is adopted to combine, be beneficial to enzyme to play a role, improve enzymolysis efficiency and nutriment stripping, improve nutritive value and the good taste of product, the interpolation of pickled vegetable fermentation liquor freeze-dried powder supplements vegetable nutrient factor vitamin C and probiotic composition, is beneficial to product and plays a role in adjustment enteron aisle.This product good mouthfeel under without the condition of flavouring, taste appraisal score value uses flavouring product 10-15 point higher than related category.
This product special flavour is delicious, and being rich in multiple nutrients functional component, is a kind of edible mushroom deep processed product collecting nutrition, nourishing, adjusting immunity of human body function.This product instant, can be used as common food flavoring or chafing dish bottom flavorings eats, also direct-edible.
Detailed description of the invention
The following examples can make the present invention of those skilled in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1
A preparation method for Chinese caterpillar fungus health powder, comprises the steps: that, by hickory chick enzymolysis powder, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder, animal protein hydrolysis powder and pickled vegetable fermentation liquor freeze-dried powder, after mixing in proportion, microwave disinfection, packaging are Chinese caterpillar fungus health powder.
The parts by weight of described Chinese caterpillar fungus health powder are composed as follows: hickory chick enzymolysis powder 15, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder 30, animal protein hydrolysis powder 3, pickled vegetable fermentation liquor freeze-dried powder 4.
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 50 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 1%, temperature 40 DEG C, pH4-5,1 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 1%, hydrolysis temperature 45-50 DEG C, pH value 6-7, enzymolysis time is after 1 hour, and adjustment temperature keeps 6 minutes to 100 DEG C, cooling; Enzymolysis liquid drying obtains hickory chick enzymolysis powder.
Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder preparation process is as follows: Chinese caterpillar fungus, after Pleurotus nebrodensis sporophore and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:5 with water, adjustment temperature is 50 DEG C, pH is 6.0, the cellulase degradation adding Chinese caterpillar fungus and Pleurotus nebrodensis sporophore quality 0.2% adjusts temperature and keeps 3 minutes to 90 DEG C after 1 hour, adjustment temperature is to 50 DEG C, interpolation Chinese caterpillar fungus and Pleurotus nebrodensis sporophore quality 0.2% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 45 DEG C, pH value 6-7, enzymolysis time 1 hour, centrifugation obtains supernatant, supernatant spraying dry obtains Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder.Described Chinese caterpillar fungus, Pleurotus nebrodensis sporophore and Radix Isatidis are all crushed to particle diameter at below 0.5mm.The parts by weight of Chinese caterpillar fungus, Pleurotus nebrodensis and Radix Isatidis are respectively Chinese caterpillar fungus 12, Pleurotus nebrodensis 10, Radix Isatidis 0.1;
Animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:6 and through milling treatment of colloid, adjustment colloid mill stator and the gap of rotor are 105-120 micron, colloid mill flow-control be 0.2-0.5 ton/hour; It is 50 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 6.0, add the papain enzymolysis 80 minutes of animal flesh quality 0.15%, 8 DEG C are cooled to keep 1.5 hours, increase the temperature to 90 DEG C to keep 20 minutes, control temperature is to 50 DEG C, add animal flesh quality 0.15% flavor protease and carry out enzymolysis, hydrolysis temperature 40 DEG C, pH value 6-7, enzymolysis time 1 hour, obtain animal protein factor digest, get centrifuged supernatant (centrifugal rotational speed 4000rpm) spraying dry and obtain animal protein hydrolysis powder.
Described animal flesh is chicken;
The enzyme preparation of adding in the present invention is purchased from Novi's letter, Beijing Dong Huadeng enzyme preparation company.
The preparation of described fermented morel liquid: adopt slant strains to spread cultivation step by step and obtain hickory chick fungal fermented filtrate;
(1) first order seed is cultivated: hickory chick slant strains accessed in 500 ml shake flasks, fermentation medium loading amount 100 milliliters, rotary shaker 180 revs/min, cultivation temperature 24 DEG C, incubation time 50 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, fermentation medium loading amount 1000 milliliters, rotary shaker 100 revs/min, cultivation temperature 24 DEG C, incubation time 50 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 10% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 24 DEG C, mixing speed 150 revs/min, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(5) secondary seed tank is cultivated: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 1 ton, and condition of culture is identical with first class seed pot;
(6) fermentation tank culture: the fermentation tank by the bacterial classification in secondary seed tank with 10% inoculum concentration access total measurement (volume) being 13 tons, fermentation medium loading amount 10 tons, cultivation temperature 24 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1:0.4, tank pressure 0.05Mpa, incubation time 72 hours.
Described above-mentioned fermented morel liquid fermentation medium consists of: millet 2%, peptone 1%, beef extract 1%, corn, and 2.5%, potassium dihydrogen phosphate 0.5%, the root of bidentate achyranthes and bark of eucommia enzymolysis liquid 10%; Insufficient section water is supplied, pH7.0-7.2,121 DEG C of high pressure steam sterilization 20min.
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 orders, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 60 DEG C, pH is 7.0, keeps 60 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
Described pickled vegetable fermentation liquor method for preparing freeze-dried powder: get 100 kilograms of pickled vegetable fermentation liquors, pH value 4.0; (1) filter: aperture is the large grain materials such as the dish leaf in the membrane filtration removing zymotic fluid of 0.5 millimeter; (2) film concentrating and separating: fermented liquid control temperature carries out UF membrane below 30 DEG C; UF membrane adopts the board-like film of 400 dalton's molecular cut offs, membrane area 1.08m
2, the filtration later stage adds about 2.5L pure water and carries out diafiltration, obtains trapped fluid 4.5kg.(3) dry powder process; Trapped fluid adopts the drying means powder process such as freeze drying.
In order to extend storage life, pack after preferably carrying out freeze drying; Cryodesiccated condition is :-40 degree pre-freezes 6 hours; Then freeze-drying is until water content is less than 5%.The method freeze drying obtains pulvis, and storage life can reach 12 months, and the reduction of microbial inoculum activity is no more than 8%.
Product preparation process of the present invention is as follows:
Take each raw material by formula, mix rear microwave disinfection, packaging is product.
Embodiment 2:
Basic with embodiment 1
A kind of Chinese caterpillar fungus health powder, parts by weight are composed as follows: hickory chick enzymolysis powder 10, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder 35, animal protein hydrolysis powder 5, pickled vegetable fermentation liquor freeze-dried powder 6.
Embodiment 3
Basic with embodiment 1
A kind of Chinese caterpillar fungus health powder, parts by weight are composed as follows: hickory chick enzymolysis powder 25, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder 25, animal protein hydrolysis powder 2, pickled vegetable fermentation liquor freeze-dried powder 2.
Product preparation process of the present invention is as follows:
Take each raw material by formula, mix rear microwave disinfection, packaging is product.
This product embodiments 1 product select in the winter time Efficiency in Buildings in Tianjin Area 100 ages 60-75 year weak easy catching a cold the elderly's edible January continuously, edible 80 grams of every day, with edible front and edible rear effectiveness comparison, the results are shown in Table 1, result shows that this product has positive effect to raising the elderly resistance, enhancing body immunity, and product has good edible and nutritious tonifying effect.Many continuously edible this product old men reflect that to take effect for a long time better, and the whole year has no interest, and health is better.
The table 1 eating effect table of comparisons
Claims (3)
1. the preparation method of a Chinese caterpillar fungus health powder, it is characterized in that, comprise the steps: that, by hickory chick enzymolysis powder, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder, animal protein hydrolysis powder and pickled vegetable fermentation liquor freeze-dried powder, after mixing in proportion, microwave disinfection, packaging are Chinese caterpillar fungus health powder;
The parts by weight of described Chinese caterpillar fungus health powder consist of: hickory chick enzymolysis powder 10-25, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder 25-35, animal protein hydrolysis powder 2-5, pickled vegetable fermentation liquor freeze-dried powder 2-6;
The preparation process of hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 40-60 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 0.5-2%, temperature 30-50 DEG C, pH4-5, time 0.5-1 hour; Add the flavor protease enzymolysis processing of zymotic fluid weight 0.3-1%, hydrolysis temperature 45-50 DEG C, pH value 6-7, after enzymolysis time 1-2 hour; Adjustment temperature keeps 5-10 minute to 90-110 DEG C, cooling; Enzymolysis liquid drying obtains hickory chick enzymolysis powder;
Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder preparation process is as follows: Chinese caterpillar fungus, after Pleurotus nebrodensis sporophore and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:5 with water, adjustment temperature is 50 DEG C, pH is 6.0, the cellulase degradation adding Chinese caterpillar fungus and Pleurotus nebrodensis sporophore quality 0.2% adjusts temperature and keeps 3 minutes to 90 DEG C after 1 hour, adjustment temperature is to 50 DEG C, interpolation Chinese caterpillar fungus and Pleurotus nebrodensis sporophore quality 0.2% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 45 DEG C, pH value 6-7, enzymolysis time 1 hour, centrifugation obtains supernatant, supernatant spraying dry obtains Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder, described Chinese caterpillar fungus, Pleurotus nebrodensis sporophore and Radix Isatidis are all crushed to particle diameter at below 0.5mm, the parts by weight of Chinese caterpillar fungus, Pleurotus nebrodensis and Radix Isatidis are respectively Chinese caterpillar fungus 12, Pleurotus nebrodensis 10, Radix Isatidis 0.1,
The root of bidentate achyranthes and the bark of eucommia enzymolysis liquid of 10-20% is added in fermentation medium in toadstool ferment process; The root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 orders, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 40-60 DEG C, pH is 5.0-7.0, keeps 30-60 minute, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid.
2. the preparation method of Chinese caterpillar fungus health powder according to claim 1, is characterized in that, described pickled vegetable fermentation liquor method for preparing freeze-dried powder: get 100 kilograms of pickled vegetable fermentation liquors, pH value 4.0; (1) filter: aperture is the large grain materials such as the dish leaf in the membrane filtration removing zymotic fluid of 0.5 millimeter; (2) film concentrating and separating: fermented liquid control temperature carries out UF membrane below 30 DEG C; UF membrane adopts the board-like film of 400 dalton's molecular cut offs, membrane area 1.08m
2, the filtration later stage adds 2.5L pure water and carries out diafiltration, obtains trapped fluid 4.5kg; (3) dry powder process; Trapped fluid adopts the powder process of freeze drying drying means; Pack after freeze drying; Described cryodesiccated condition is :-40 degree pre-freezes 6 hours; Then freeze-drying is until water content is less than 5%.
3. the preparation method of Chinese caterpillar fungus health powder according to claim 1, it is characterized in that, comprise the steps: that, by hickory chick enzymolysis powder, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder, animal protein hydrolysis powder and pickled vegetable fermentation liquor freeze-dried powder, after mixing in proportion, microwave disinfection, packaging are Chinese caterpillar fungus health powder;
The parts by weight of described Chinese caterpillar fungus health powder are composed as follows: hickory chick enzymolysis powder 15, Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder 30, animal protein hydrolysis powder 3, pickled vegetable fermentation liquor freeze-dried powder 4;
The preparation process of described hickory chick enzymolysis powder is as follows: hickory chick prepares through fermentation method, adjusts to 50 DEG C after fermentation ends, adds the cellulase degradation process of zymotic fluid weight 1%, temperature 40 DEG C, pH4-5,1 hour time; Add the flavor protease enzymolysis processing of zymotic fluid weight 1%, hydrolysis temperature 45-50 DEG C, pH value 6-7, enzymolysis time is after 1 hour, and adjustment temperature keeps 6 minutes to 100 DEG C, cooling; Enzymolysis liquid drying obtains hickory chick enzymolysis powder;
Described fermented morel liquid fermentation medium consists of: millet 2%, peptone 1%, beef extract 1%, corn 2.5%, potassium dihydrogen phosphate 0.5%, the root of bidentate achyranthes and bark of eucommia enzymolysis liquid 10%; Insufficient section water is supplied, pH7.0-7.2,121 DEG C of high pressure steam sterilization 20min;
The described root of bidentate achyranthes and bark of eucommia enzymolysis liquid preparation method as follows: the interpolation mass ratio of the root of bidentate achyranthes and the bark of eucommia is 1:2, both were pulverized 100 orders, crushed mixture is added 6 times of water-soluble solutions, the cellulase degradation of interpolation mixture quality 2.5% 35 minutes, adjustment temperature is 60 DEG C, pH is 7.0, keeps 60 minutes, obtains the root of bidentate achyranthes and bark of eucommia enzymolysis liquid;
Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder preparation process is as follows: Chinese caterpillar fungus, after Pleurotus nebrodensis sporophore and Radix Isatidis are pulverized, crushed material mixes with the ratio of 1:5 with water, adjustment temperature is 50 DEG C, pH is 6.0, the cellulase degradation adding Chinese caterpillar fungus and Pleurotus nebrodensis sporophore quality 0.2% adjusts temperature and keeps 3 minutes to 90 DEG C after 1 hour, adjustment temperature is to 50 DEG C, interpolation Chinese caterpillar fungus and Pleurotus nebrodensis sporophore quality 0.2% flavor protease carry out enzymolysis subsequently, hydrolysis temperature 45 DEG C, pH value 6-7, enzymolysis time 1 hour, centrifugation obtains supernatant, supernatant spraying dry obtains Pleurotus nebrodensis Chinese caterpillar fungus enzymolysis powder, described Chinese caterpillar fungus, Pleurotus nebrodensis sporophore and Radix Isatidis are all crushed to particle diameter at below 0.5mm, the parts by weight of Chinese caterpillar fungus, Pleurotus nebrodensis and Radix Isatidis are respectively Chinese caterpillar fungus 12, Pleurotus nebrodensis 10, Radix Isatidis 0.1,
Animal protein hydrolysis powder, preparation method thereof is as follows: get quantitative animal flesh and rub through meat grinder, animal flesh after rubbing and water mix with the ratio of 1:6 and through milling treatment of colloid, adjustment colloid mill stator and the gap of rotor are 105-120 micron, colloid mill flow-control be 0.2-0.5 ton/hour; It is 50 DEG C that animal flesh after milling treatment of colloid is adjusted temperature, pH is 6.0, add the papain enzymolysis 80 minutes of animal flesh quality 0.15%, 8 DEG C are cooled to keep 1.5 hours, increase the temperature to 90 DEG C to keep 20 minutes, control temperature is to 50 DEG C, add animal flesh quality 0.15% flavor protease and carry out enzymolysis, hydrolysis temperature 40 DEG C, pH value 6-7, enzymolysis time 1 hour, obtains animal protein factor digest, gets centrifuged supernatant, centrifugal rotational speed 4000rpm, spraying dry obtains animal protein hydrolysis powder;
Described pickled vegetable fermentation liquor method for preparing freeze-dried powder: get 100 kilograms of pickled vegetable fermentation liquors, pH value 4.0; (1) filter: aperture is the large grain materials such as the dish leaf in the membrane filtration removing zymotic fluid of 0.5 millimeter; (2) film concentrating and separating: fermented liquid control temperature carries out UF membrane below 30 DEG C; UF membrane adopts the board-like film of 400 dalton's molecular cut offs, membrane area 1.08m
2, the filtration later stage adds 2.5L pure water and carries out diafiltration, obtains trapped fluid 4.5kg; (3) dry powder process; Trapped fluid adopts the powder process of freeze drying drying means;
Described cryodesiccated condition is :-40 degree pre-freezes 6 hours; Then freeze-drying is until water content is less than 5%.
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| CN102960789A (en) * | 2012-10-21 | 2013-03-13 | 山西汉波食品股份有限公司 | Isatis root and red date concentrated slurry product |
| CN103504444A (en) * | 2012-06-16 | 2014-01-15 | 唐桂德 | Method for preparing mythic fungus and dictyophora phalloidea solid beverage |
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| CN103504444A (en) * | 2012-06-16 | 2014-01-15 | 唐桂德 | Method for preparing mythic fungus and dictyophora phalloidea solid beverage |
| CN102960789A (en) * | 2012-10-21 | 2013-03-13 | 山西汉波食品股份有限公司 | Isatis root and red date concentrated slurry product |
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