CN104138897A - Reinforcement restoration method using hyperaccumulation restoration plant for restoration of cadmium contaminated soil - Google Patents
Reinforcement restoration method using hyperaccumulation restoration plant for restoration of cadmium contaminated soil Download PDFInfo
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- CN104138897A CN104138897A CN201310167548.0A CN201310167548A CN104138897A CN 104138897 A CN104138897 A CN 104138897A CN 201310167548 A CN201310167548 A CN 201310167548A CN 104138897 A CN104138897 A CN 104138897A
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- 239000002689 soil Substances 0.000 title claims abstract description 64
- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 32
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000002787 reinforcement Effects 0.000 title abstract 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 13
- 108010024636 Glutathione Proteins 0.000 claims abstract description 12
- 241000196324 Embryophyta Species 0.000 claims description 28
- 230000008439 repair process Effects 0.000 claims description 22
- 238000005728 strengthening Methods 0.000 claims description 12
- 235000002594 Solanum nigrum Nutrition 0.000 claims description 9
- 240000002307 Solanum ptychanthum Species 0.000 claims description 9
- 239000003337 fertilizer Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 238000005553 drilling Methods 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 238000009331 sowing Methods 0.000 abstract description 3
- 229960003180 glutathione Drugs 0.000 abstract 1
- 238000003971 tillage Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002686 phosphate fertilizer Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000010205 Cola acuminata Nutrition 0.000 description 1
- 244000228088 Cola acuminata Species 0.000 description 1
- 235000015438 Cola nitida Nutrition 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000511303 Gammarus fossarum Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000007202 Nickl synthesis reaction Methods 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
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- 231100000160 chronic toxicity Toxicity 0.000 description 1
- 231100000749 chronicity Toxicity 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 231100000584 environmental toxicity Toxicity 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
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- 230000036542 oxidative stress Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
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Abstract
The invention relates to a method for phytoremediation of cadmium contaminated soil, in particular relates to a reinforcement restoration method using a hyperaccumulation restoration plant for restoration of the cadmium contaminated soil. The hyperaccumulation restoration plant is sowed in ridge tillage and row planting manner in the cadmium contaminated soil in spring, the sowing dosage is 1.8-2.2 grams of seeds in every square metre, the row spacing is 48 to 52 meters, after seedling emergence, when the cadmium hyperaccumulation restoration plant height is about 20cm, levo reduced form glutathione is applied, the applying dose is 0.1-1.0mmol / kg soil, and the applying concentration per kg soil is measured by using the weight of the soil with the soil layer thickness of 0-20 cm of the soil layer surface of the contaminated soil.
Description
Technical field
The present invention relates to the method for the phytoremediation of cadmium pollution soil, specifically a kind of strengthening repair method that utilizes super enrichment rehabilitation plant cadmium pollution soil repair.
Background technology
According to China environmental aspect communique report in 1997, China ploughs had more than 1,000 ten thousand hm2 to be subject to multiple pollution in various degree, and its area accounts for 7.34% of cultivated area then.2000 to the whole nation 300,000 hm
2the sample investigation of Basic Farmland Conservation Zones soil harmful heavy metal show, heavy metals exceeding standard rate reaches 12.1%; There is 891 of agricultural environment pollution accidents in 23 provinces (urban district), polluted agricultural land 40,000 hm altogether
2, direct economic loss reaches 2.2 hundred million yuan, is seriously restricting the national economic development and is endangering people's physical and mental health.Variation along with pollutant emission, complicated and frequentization, the contaminated degree of major receptors soil of pollutant increases the weight of day by day, the complexity that contaminated soil is repaired and refractory rationality feature be outstanding all the more (document 1:Paton GI also, Viventsova E, Kumpene J, et al.2006.An ecotoxicity assessment of contaminated forest soils from the Kola Peninsula.Science of the Total Environment, 355 (13): 106-117), wherein the pollution of heavy metal-organic composite is more common pollution type, as Cd-polycyclic aromatic hydrocarbon (Polycyclic aromatic hydrocarbons, PAHs) combined pollution.Cadmium can be by reducing photosynthesis of plant when low concentration, produce the approach such as oxidative stress suppress plant growth, to plant produce toxic action (document 2:Schill RO,
2003.Laboratory simulation of a mining accident:acute toxicity hsc/hsp70response and recovery from sress in Gammarus fossarum (Crustacea smphipoda) exposed to a pulse of cadmium.Biometals, 16:391-401), and to some extent humans and animals is produced to toxic action, also can bring out canceration; And PAHs is a class persistence organic pollutant, biology is had to chronic toxicity and carcinogenic, teratogenesis, mutagenicity.Because Cd, PAHs show as in soil environment, have disguise and chronicity, cause the difficulty of repairing this type of combined contamination soil to strengthen, seeking efficient, safe, economic recovery technique becomes the realistic problem of needing solution badly.Phytoremediation technology have the incomparable expense of physics, chemical repair method cheap, be difficult for causing secondary pollution, beautify the environment, be easy to as advantages such as society are accepted; become extremely potential recovery technique (document 3:Chaney RL; Malik M; Li YM.1997.Phytoremediation of soil metals.Current Opinions in Biotechnology, 8:279-284; Brooks RR, Chambers MF, Nickls L J, et al.1998.Phytomining.Trends in Plant Science, 3 (9): 359-362).The phytoremediation of heavy-metal contaminated soil is mainly to be removed and be used for realizing (document 4: Wei Shuhe by the excess of super enriching plant or enriching plant, Zhou Qixing, Pavel V.Koval, Galina A.Belogolova. Phytoremediation of organic pollutants-contaminated environment: A research review. ecological magazine .2006,25 (6): 716-721); For organic contamination as polycyclic aromatic hydrocarbon be mainly to utilize the degradation of plant rhizosphere circle microorganism to complete (document 4: Wei Shuhe, Zhou Qixing, Pavel V.Koval, Galina A.Belogolova. Phytoremediation of organic pollutants-contaminated environment: A research review. ecological magazine .2006,25 (6): 716-721).But it is restricted with some that phytoremediation technology also exists some defects, as being very easily subject to, the own biological characteristics of environmental condition and plant affects, repairing efficiency is long etc.Therefore need to adopt some strengthening reclamation activities to improve the efficiency of phytoremediation, to improving reference is provided early for this technology.
Summary of the invention
The object of the present invention is to provide a kind of strengthening repair method that utilizes super enrichment rehabilitation plant cadmium pollution soil repair.
For achieving the above object, the technical solution used in the present invention is:
Utilize a strengthening repair method for super enrichment rehabilitation plant cadmium pollution soil repair,
Plant Cd-hyperaccumulator in the mode of ridge culture drilling spring on by the soil of cadmium pollution, application rate is every square metre of 1.8-2.2 gram seed, line-spacing is 48-52 rice, after emerging, when the about 20cm of Cd-hyperaccumulator plant height, apply left-handed reduced glutathione, applying dosage is 0.1-1.0mmol/kg soil.
When described left-handed reduced glutathione is used, left-handed reduced glutathione powder is mixed with soil, before light rain arriving, evenly spread fertilizer over the fields in soil surface, or left-handed reduced glutathione and water are uniformly dissolved and are spread fertilizer over the fields in soil surface.
When Cd-hyperaccumulator is ripe, gather in the crops whole strain plant, band is eradicated and is removed, and the Cd-hyperaccumulator after results is transferred to other place and focuses on or carry out resource processing and process.
Described Cd-hyperaccumulator is black nightshade
Referring to the first tenday period of a month in late April to May described spring, is that late July is to early August during results black nightshade.Because phosphate fertilizer has to cadmium the extraction reparation that chelating precipitation is not utilized cadmium, so while planting Cd-hyperaccumulator black nightshade spring, bottom application in spring be take and executed phosphate fertilizer as 25-35 kilogram/667 meters
2be advisable; Referring to the first tenday period of a month in late April to May described spring, is that late July is to early August during results black nightshade.
The present invention utilizes super enriching plant from cadmium pollution soil, to take away a large amount of cadmiums, makes contaminated soil repaired.
It is that every kg soil is with the soil weight metering of the 0-20 centimetre of dark soil thickness in contaminated soil soil layer surface that described left-handed reduced glutathione applies concentration.
The present invention has advantages of:
The present invention's operation is relatively simple, and the hardening agent cost applying is lower, and repairing effect is better, to soil, also can not produce secondary pollution, increases in addition the effect of phytomass simultaneously.
The specific embodiment
Field experiment is repaired in the strengthening of embodiment cadmium pollution soil
1. test site and step
In Zhang Shi irrigated area, Shenyang, carry out relevant field experiment, total Test is tested approximately 50 meters of the gross areas
2.This ground physiochemical properties of soil: pH6.5, organic carbon 20.86g/Kg, full nitrogen 1.32g/Kg, full phosphorus 0.64g/Kg, available nitrogen 179.63mg/Kg, rapid available phosphorus 2.78mg/Kg.Top layer (0-20 centimetre) cadmium content mean value is 0.65mg/Kg, with China's standard of soil environment quality secondary standard, belongs to cadmium pollution soil.
Late April starts test, every 667 meters of soil
230 kilograms, bottom application ammonium sulfate.Sowing at 4 the end of month, soaks seed 12 hours with 0.1% hydrogen peroxide before sowing.Black nightshade, apply the left-handed reduced glutathione of low dosage (0.1-1.0mmol/kg) seedling stage, first with a certain amount of soil and reagent mix, then evenly spread fertilizer over the fields in soil surface.Be selected in and use the previous day of spotting.After black nightshade maturation, measure soil and plant sample, to detect the repairing effect of contaminated soil simultaneously.Adopt diagonal 5 point sampling methods.
2. sample analysis
A plant sample part for results is for the mensuration of overground part content of beary metal, and remainder root division, stem, leaf, fruit 4 parts, fully rinse with running water respectively, and then with deionized water rinsing, drain moisture, the 30min that completes at 105 ℃ then dries to constant weight at 70 ℃ in baking oven.Plant sample after oven dry pulverizes and sieves standby, and air-dry rear mistake 100 mesh sieves of pedotheque are standby.Plant and pedotheque all adopt HNO
3-HClO
4method digestion (the two volume ratio is 3:1), atomic absorption spectrophotometer method is measured the content of Cd, and atomic absorption spectrophotometer is the 180-80 of Hitachi, and the absorbing wavelength of cadmium is 228.8.The mensuration of the basic physical and chemical such as the content of organic matter of soil adopts conventional assay method.
3 data statistics
The data that obtain are carried out statistical analysis with Microsoft Excel and SPSS16.0, utilize Duncan newly to answer range test and carry out significance test of difference (p<0.05).
4. result
Result of the test shows (table 1), through black nightshade and the Chinese Cabbage in a season, and cadmium content average out to 0.68mg/kg in upper soll layer, the extraction repair rate of cadmium is 8.78%;
Sampled point cadmium content (mg/kg) in table 1 field experiment
| Sampled point | 1 | 2 | 3 | 4 | 5 | On average |
| Before Cadmium in Soil is repaired | 0.62 | 0.71 | 0.64 | 0.65 | 0.68 | 0.66 |
| After Cadmium in Soil is repaired | 0.58 | 0.62 | 0.58 | 0.62 | 0.61 | 0.60 |
Claims (6)
1. utilize a strengthening repair method for super enrichment rehabilitation plant cadmium pollution soil repair, it is characterized in that:
Plant Cd-hyperaccumulator in the mode of ridge culture drilling spring on by the soil of cadmium pollution, application rate is every square metre of 1.8-2.2 gram seed, line-spacing is 48-52 rice, after emerging, when the about 20cm of Cd-hyperaccumulator plant height, apply left-handed reduced glutathione, applying dosage is 0.1-1.0mmol/kg soil.
2. by the strengthening repair method of the super enrichment rehabilitation plant of utilization claimed in claim 1 cadmium pollution soil repair, it is characterized in that: when described left-handed reduced glutathione is used, left-handed reduced glutathione powder is mixed with soil, before light rain arrives, evenly spread fertilizer over the fields in soil surface, or left-handed reduced glutathione and water are uniformly dissolved and are spread fertilizer over the fields in soil surface.
3. by the strengthening repair method of the super enrichment rehabilitation plant of utilization claimed in claim 1 cadmium pollution soil repair, it is characterized in that: when Cd-hyperaccumulator is ripe, gather in the crops whole strain plant, band is eradicated and is removed, and the Cd-hyperaccumulator after results is transferred to other place and focuses on or carry out resource processing and process.
4. by the strengthening repair method of the super enrichment rehabilitation plant of the utilization cadmium pollution soil repair described in claim 1 or 3, it is characterized in that: described Cd-hyperaccumulator is black nightshade.
5. by the strengthening repair method of the super enrichment rehabilitation plant of utilization claimed in claim 1 cadmium pollution soil repair, it is characterized in that: referring to the first tenday period of a month in late April to May described spring, is that late July is to early August during results black nightshade.
6. by the strengthening repair method of the super enrichment rehabilitation plant of utilization claimed in claim 1 cadmium pollution soil repair, it is characterized in that: it is that every kg soil is with the soil weight metering of the 0-20 centimetre of dark soil thickness in contaminated soil soil layer surface that described left-handed reduced glutathione applies concentration.
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| CN201310167548.0A CN104138897A (en) | 2013-05-08 | 2013-05-08 | Reinforcement restoration method using hyperaccumulation restoration plant for restoration of cadmium contaminated soil |
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| CN201310167548.0A CN104138897A (en) | 2013-05-08 | 2013-05-08 | Reinforcement restoration method using hyperaccumulation restoration plant for restoration of cadmium contaminated soil |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472175A (en) * | 2014-12-01 | 2015-04-01 | 四川农业大学 | Method for improving cadmium-contaminated orchard soil remediation capability of solanum photeinocarpum |
| CN111530919A (en) * | 2020-04-30 | 2020-08-14 | 上海交通大学 | Optimal phytoremediation method of cadmium and arsenic composite polluted soil based on Solanum nigrum |
| CN113600609A (en) * | 2021-07-30 | 2021-11-05 | 山东省农业科学院 | Crop restoration method for reducing cadmium content in soil |
| CN116871316A (en) * | 2023-07-04 | 2023-10-13 | 江南大学 | A method to reduce the content of heavy metal cadmium in soil |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472175A (en) * | 2014-12-01 | 2015-04-01 | 四川农业大学 | Method for improving cadmium-contaminated orchard soil remediation capability of solanum photeinocarpum |
| CN111530919A (en) * | 2020-04-30 | 2020-08-14 | 上海交通大学 | Optimal phytoremediation method of cadmium and arsenic composite polluted soil based on Solanum nigrum |
| CN113600609A (en) * | 2021-07-30 | 2021-11-05 | 山东省农业科学院 | Crop restoration method for reducing cadmium content in soil |
| CN116871316A (en) * | 2023-07-04 | 2023-10-13 | 江南大学 | A method to reduce the content of heavy metal cadmium in soil |
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Application publication date: 20141112 |