CN104137764B - The selection of high resistance naked body yellow leaf Characters of Giant Embryo Rice CMS line - Google Patents

Abstract

The invention discloses a method for breeding a three-line sterile line of high-resistance light-bodied yellow-leaf giant-embryo rice and belongs to the technical field of special rice breeding. In this method, the yellow leaf marker parent, drought-resistant parent, naked rice parent, and rice blast-resistant parent are selected as hybrid parents, and the three-line maintainer geB of giant embryo rice is selected as recurrent parent. The genes of the four traits of drought resistance and rice blast resistance were aggregated into the three-line maintainer line of giant embryo rice, and the agronomic and yield traits were highly consistent with the original three-line maintainer geB of giant embryo rice, with light body, yellow leaves, and drought resistance. , the giant embryo three-line maintainer line (anti-yellow light geB) with the characteristics of rice blast resistance, and the anti-yellow light geB was crossed with the corresponding giant embryo rice male sterile line and backcrossed for 3 generations, and bred with light body, yellow leaves, drought resistance, The giant embryo three-line CMS line (anti-yellow light geA) with the characteristics of rice blast resistance has the advantages of good resistance, high purity and easy harvesting.

Description

Breeding Method of Three-line CMS Line of Light Body Yellow Leaf Giant Embryo Rice with High Resistance

technical field

The invention specifically relates to a method for breeding a three-line sterile line of high-resistance light-bodied yellow-leaf giant-embryo rice and belongs to the technical field of special rice breeding.

Background technique

The recessive leaf color marker yellow leaf marker is introduced into the sterile line to assist in the removal of hybrids, which is an effective way to improve the purity of hybrids. Breeders use the CMS seedlings to carry yellow leaf markers, and the hybrid F ₁ they prepare shows normal green characteristics. At the F ₁ seedling stage, the CMS or maintainer line seedlings mixed with it are eliminated according to the difference in leaf color, so as to achieve the goal of removing Miscellaneous protection pure purpose. Naked rice is a kind of rice with no hair all over the body. Because it is not easy for pests to attach and invade, it has strong resistance to pests and diseases. It can reduce skin itching and damage to the skin during manual harvesting and threshing, and is easy to mechanized harvesting and processing. There is no dust at all times, which can reduce environmental pollution, so it is also called "environmentally friendly rice". Giant embryo rice is a kind of rice with a huge embryo. The nutritional value of rice is greatly improved due to the enlargement of the embryo. It is rich in γ-aminobutyric acid, protein and various minerals, amino acids, vitamins and other natural nutrients. And physiologically active substances, regular food can improve health and regulate human circadian rhythm. In recent years, there have been many natural disasters in the world. The shortage of water resources and the serious occurrence of rice blast directly affect the production of rice. Drought and rice blast have become one of the main reasons for the loss of rice production. It is of great practical significance to cultivate three-line sterile lines and hybrid rice that are resistant to drought and rice blast, and have the traits of bare body, yellow leaves, and giant embryo. The method of the present invention aggregates a variety of rice stress resistance and beneficial special traits genes into the three-line maintainer material of giant embryo rice by hybrid backcross polymerization method, and then crosses it with the corresponding giant embryo rice male sterile line to breed The three-line male sterile line of the light-body yellow-leaf giant-embryo rice with high resistance can be used to prepare the three-line giant-embryo hybrid rice, which can improve the stress resistance level, improve the purity of the hybrid, facilitate mechanized harvesting and increase the yield level of the three-line giant-embryo hybrid rice.

Contents of the invention

The object of the present invention is to provide a method for breeding a three-line sterile line of giant embryo rice with high resistance to naked yellow leaves. The four genes of drought resistance, rice blast, naked body and yellow leaves are aggregated into giant embryo rice by hybridization and backcross polymerization. Among the three-line sterile lines of embryo rice, a three-line sterile line of giant embryo rice with the characteristics of light body, yellow leaves, drought resistance and rice blast resistance was bred.

To achieve the above object, the present invention adopts the following technical solutions:

A method for breeding a three-line sterile line of light-bodied yellow-leaf giant-embryo rice with high resistance, comprising the following steps:

1) Select yellow leaf marker parent A, drought-resistant variety B, naked rice parent C, rice blast-resistant parent D as the source of resistance, and the three-line maintainer of giant embryo rice as the recurrent parent E, and make crosses A/B and C/D , to obtain hybrid F ₁ generations, which are represented by F _1-AB and F _1-CD respectively;

2) Plant F _1-AB and F _1-CD , and cross F _1-AB /F _1-CD at the flowering stage to obtain recross F _1-ABCD ;

3) Plant F _1-ABCD , self-cross to get F _2-ABCD , plant F _2-ABCD and the recurrent parent E, the F _2-ABCD population is 900-1000 plants, and yellow leaves are carried out at the three-leaf one-center to four-leaf stage at the seedling stage. Screening of traits and bareness traits, select seedlings with both yellow leaves and bareness traits to transplant to the same field, conduct screening tests for resistance to drought and rice blast, select seedlings that are resistant to both drought and rice blast to be listed and marked, and choose to be listed for marking at the flowering stage And a single plant with excellent agronomic and yield traits can be crossed with E/F _2-ABCD to get F _1-ABCDE ;

4) Plant the F _1-ABCDE obtained in 3), and self-cross to obtain F _2-ABCDE , dehull the F _2-ABCDE seeds into brown rice, and select the brown rice with giant embryo traits for sowing. The population is 400-500 plants. Screening of yellow leaf traits and naked traits is carried out at the stage of one leaf to four leaves, and seedlings with both yellow leaf and naked traits are selected for transplantation to the same field, and screening tests for resistance to drought and rice blast are carried out. The seedlings are listed and marked, and the individual plants with listed markings and agronomic and yield traits similar to the recurrent parent E are selected at the flowering stage, and backcross E/F _2-ABCDE to obtain BC ₁ F ₁ ;

5) Plant BC ₁ F ₁ obtained from 4) and self-cross to obtain BC ₁ F ₂ , plant BC ₁ F ₂ in a population of 400-500 plants, and carry out yellow leaf traits and bare body traits at the three-leaf one-heart to four-leaf stage at the seedling stage Screening, select seedlings with both yellow leaves and bare body traits to transplant to the same field, conduct screening tests for resistance to drought and rice blast, select seedlings that are resistant to drought and rice blast at the same time, and select the seedlings that are marked at the flowering stage and have agronomic and yield traits A single plant similar to the recurrent parent E, backcrossed with the recurrent parent E to obtain BC ₂ F ₁ ;

6) Continue to backcross to BC ₄ F ₁ according to the procedure in step 5), and self-cross to obtain BC ₄ F ₂ generations, plant BC ₄ F ₂ generations, and plant the giant embryo male sterile line corresponding to the recurrent parent E at the same time, the seedling stage is opposite to BC ₄ F ₂ generations were screened for yellow leaf traits, bare body traits, drought resistance, and rice blast resistance according to step 5), and listed rice seedlings with yellow leaf traits, naked body traits, drought resistance, and rice blast resistance. Among the marked rice plants, the single plant with excellent agronomic characteristics and the most similar to the recurrent parent E was selected as the high-resistant bare yellow-leaf giant embryo rice maintainer line;

7) The maintainer line of rice with high resistance to giant embryo rice was crossed with the giant embryo male sterile line and backcrossed for 3 generations, that is, a three-line male sterile line with high resistance to giant embryo rice with light body and yellow leaves was bred.

The recurrent parent E is 1813geB, TgeB or 98-14geB, and its corresponding macroembryo male sterile line is 1813geA, TgeA or 98-14geA.

The trait of megaembryo is that the size of the embryo is more than twice the size of the common rice embryo.

The yellow leaf marker parent A is Huangyu B, the drought-resistant parent B is Luhan 1, the naked rice parent C is Guangxiang B, and the blast-resistant parent D is Wunong 1.

The three-line CMS line of rice with high resistance to naked yellow leaves and giant embryos has the characteristics of giant embryo traits, naked traits, yellow leaf traits, drought resistance, and rice blast resistance.

Beneficial effects of the present invention:

The effect of the present invention is that the traits of yellow leaf mark, drought resistance, bare body and rice blast resistance are aggregated into three-line male sterile lines of giant embryo rice respectively, and a three-line male sterile line of giant embryo rice with high resistance to light body and yellow leaves is bred, which is used to prepare three The line of giant embryo hybrid rice can improve the resistance level of drought resistance and rice blast, the yellow leaf mark is easy to remove hybrid plants, improve the purity of hybrids, and the bare body trait is easy to mechanized harvesting and increase the yield level of three-line giant embryo hybrid rice. The yield per mu of the three-line giant-embryo hybrid rice prepared with the three-line giant-embryo rice CMS line with high resistance light body yellow leaves is 15-20% higher than that of the three-line giant-embryo hybrid rice control variety prepared with the original giant-embryo rice three-line sterile line.

detailed description

Example 1:

1) The yellow leaf marker parent Huangyu B (A), the drought-resistant variety Luhan 1 (B), the naked rice parent Guangxiang B (C), and the rice blast-resistant parent Wunong 1 (D) were selected as resistant parents, The three-line maintainer line 1813geB of giant embryo rice is the recurrent parent (E), and the crosses A/B and C/D are used to obtain the hybrid F ₁ generation, which are represented by F _1-AB and F _1-CD respectively;

2) Plant F _1-AB and F _1-CD , and cross F _1-AB /F _1-CD at the flowering stage to obtain recross F ₁ (F _1-ABCD );

3) F _1-ABCD was planted, F 2 _-ABCD was obtained by self-crossing, F _2-ABCD and the recurrent parent 1813geB were planted, the F _2-ABCD population was 900 plants, and yellow leaf traits, Screening of naked traits, selecting seedlings with both yellow leaves and naked traits to transplant to the same field, conducting screening tests for resistance to drought and rice blast, selecting seedlings resistant to both drought and rice blast to be marked, and selected for flowering period to be marked and agronomically F _1-ABCDE was obtained by crossing 1813geB/F _2-ABCD with 5 individual plants with excellent yield traits;

4) Plant the F _1-ABCDE obtained in 3), and self-cross to obtain F _2-ABCDE , dehull the F _2-ABCDE seeds into brown rice, select the brown rice with giant embryo traits for sowing, the population is 400 plants, and the three leaves are one heart At the four-leaf stage, screen for yellow leaf traits and naked traits, select seedlings with both yellow leaf and naked traits to transplant to the same field, conduct screening tests for resistance to drought and rice blast, and select seedlings that are resistant to both drought and rice blast to be listed BC ₁ F _{1 ;}

5) Plant BC ₁ F ₁ obtained from 4) and self-cross to obtain BC ₁ F ₂ , plant BC ₁ F ₂ in a population of 400 plants, and screen for yellow leaf traits and bare body traits at the three-leaf one-heart to four-leaf stage at the seedling stage, Select seedlings with both yellow leaves and bare body traits to transplant to the same field, and carry out screening tests for resistance to drought and rice blast. Select seedlings that are resistant to both drought and rice blast to be marked, and the flowering stage is selected to be marked and the agronomic and yield traits are consistent with 1813geB 5 similar single plants were backcrossed with 1813geB to obtain BC ₂ F ₁ ;

6) Continue to backcross to BC ₄ F ₁ according to the procedure in step 5), and self-cross to obtain BC ₄ F ₂ generations, plant BC ₄ F ₂ generations, and plant the giant embryo male sterile line 1813geA corresponding to 1813geB at the seedling stage to BC ₄ F ₂ generations were screened for yellow leaf traits, naked traits, drought resistance, and rice blast resistance (according to step 5), and listed rice seedlings with yellow leaf and naked traits, drought resistance, and rice blast resistance. Select 5 single plants with excellent agronomic traits and the most similarity to 1813geB among the marked rice plants, and then they can be established as the high-resistance light-body yellow-leaf giant embryo rice maintainer line "Anti-Huangguang 1813geB";

7) "Kanghuangguang 1813geB" was crossed with the giant embryo male sterile line 1813geA corresponding to 1813geB and backcrossed for 3 generations, and the three-line male sterile line "Kanghuangguang 1813geA" with high resistance to light body and yellow leaf giant embryo rice could be bred.

The three-line CMS line "Kanghuangguang 1813geA" with high resistance to light-body yellow-leaf giant-embryo rice has the characteristics of giant-embryo traits, light-body traits, yellow-leaf traits, drought resistance, and rice blast resistance. It is used to prepare three-line giant-embryo hybrid rice "Anti-Huangguang 1813geA/MhgeR" is resistant to drought and rice blast, is easy to remove hybrids, improves the purity of hybrids, and is easy to harvest mechanized. %.

Example 2:

1) The yellow leaf marker parent Huangyu B (A), the drought-resistant variety Luhan 1 (B), the naked rice parent Guangxiang B (C), and the rice blast-resistant parent Wunong 1 (D) were selected as resistant parents, The three-line maintainer line TgeB of giant embryo rice is the recurrent parent (E), and crosses A/B and C/D to obtain the hybrid F ₁ generation, which are represented by F _1-AB and F _1-CD respectively;

2) Plant F _1-AB and F _1-CD , and cross F _1-AB /F _1-CD at the flowering stage to obtain recross F ₁ (F _1-ABCD );

3) F _1-ABCD was planted, F 2 _-ABCD was obtained by self-crossing, F _2-ABCD and the recurrent parent TgeB were planted, the F _2-ABCD population was 1000 plants, and the yellow leaf traits, Screening of naked traits, selecting seedlings with both yellow leaves and naked traits to transplant to the same field, conducting screening tests for resistance to drought and rice blast, selecting seedlings resistant to both drought and rice blast to be marked, and selected for flowering period to be marked and agronomically F _1-ABCDE was obtained by crossing TgeB/F _2-ABCD with 5 single plants with excellent yield traits;

4) Plant the F _1-ABCDE obtained in 3), and self-cross to obtain F _2-ABCDE , dehull the F _2-ABCDE seeds into brown rice, and select the brown rice with giant embryo traits for sowing. At the four-leaf stage, screen for yellow leaf traits and naked traits, select seedlings with both yellow leaf and naked traits to transplant to the same field, conduct screening tests for resistance to drought and rice blast, and select seedlings that are resistant to both drought and rice blast to be listed Marking, 5 individual plants with listed markers and similar agronomic and yield traits to TgeB were selected at the flowering stage, and backcrossed with TgeB/F _2-ABCDE to obtain BC ₁ F ₁ ;

5) Plant BC ₁ F ₁ obtained from 4) and self-cross to obtain BC ₁ F ₂ , plant BC ₁ F ₂ in a population of 500 plants, and screen for yellow leaf traits and bare body traits at the three-leaf one-heart to four-leaf stage at the seedling stage, Select seedlings with both yellow leaves and bare body traits to transplant to the same field, and carry out screening tests for resistance to drought and rice blast. Select seedlings that are resistant to both drought and rice blast to be marked, and the flowering stage is selected to be marked and the agronomic and yield traits are consistent with TgeB. 5 similar single plants were backcrossed with TgeB to obtain BC ₂ F ₁ ;

6) Continue to backcross to BC ₄ F ₁ according to the procedure in step 5), and self-cross to obtain BC ₄ F ₂ generations, plant BC ₄ F ₂ generations, and plant the giant embryo male sterile line TgeA corresponding to TgeB at the seedling stage to BC ₄ F F ₂ generations were screened for yellow leaf traits, naked traits, drought resistance, and rice blast resistance (according to step 5), and listed rice seedlings with yellow leaf and naked traits, drought resistance, and rice blast resistance. Select 5 individual plants with excellent agronomic traits and the most similarity to TgeB among the marked rice plants to be finalized as the high-resistance light-body yellow-leaf giant-embryo rice maintainer line "anti-yellow light TgeB";

7) "Anti-yellow light TgeB" was crossed with the giant embryo male sterile line TgeA corresponding to TgeB and backcrossed for 3 generations, and the three-line male sterile line "Anti-yellow light TgeA" with high resistance to light body and yellow leaves giant embryo rice could be bred.

The three-line CMS line "Anti-Huangguang TgeA" with high resistance to light body yellow leaf giant embryo rice has the characteristics of giant embryo traits, naked body traits, yellow leaf traits, drought resistance, and rice blast resistance. It is used to prepare three-line giant embryo hybrid rice "Anti-yellow light TgeA/MhgeR" is resistant to drought and rice blast, is easy to remove hybrids, improves the purity of hybrids, and is easy to mechanized harvesting. %.

Example 3:

1) The yellow leaf marker parent Huangyu B (A), the drought-resistant variety Luhan 1 (B), the naked rice parent Guangxiang B (C), and the rice blast-resistant parent Wunong 1 (D) were selected as resistant parents, The three-line maintainer line 98-14geB of giant embryo rice is the recurrent parent (E), and the crosses A/B and C/D are used to obtain the hybrid F ₁ generation, which are represented by F _1-AB and F _1-CD respectively;

2) Plant F _1-AB and F _1-CD , and cross F _1-AB /F _1-CD at the flowering stage to obtain recross F ₁ (F _1-ABCD );

3) Plant F _1-ABCD , self-cross to get F _2-ABCD , plant F _2-ABCD and the recurrent parent 98-14geB, the F _2-ABCD population is 950 plants, and yellow leaves are carried out at the three-leaf one-center to four-leaf stage at the seedling stage. Screening of traits and bareness traits, select seedlings with both yellow leaves and bareness traits to transplant to the same field, conduct screening tests for resistance to drought and rice blast, select seedlings that are resistant to both drought and rice blast to be listed and marked, and choose to be listed for marking at the flowering stage And 5 individual plants with excellent agronomic and yield traits were crossed with 98-14geB/F _2-ABCD to obtain F _1-ABCDE ;

4) Plant the F _1-ABCDE obtained in 3), and self-cross to obtain F _2-ABCDE , dehull the F _2-ABCDE seeds into brown rice, and select the brown rice with giant embryo traits for sowing. At the four-leaf stage, screen for yellow leaf traits and naked traits, select seedlings with both yellow leaf and naked traits to transplant to the same field, conduct screening tests for resistance to drought and rice blast, and select seedlings that are resistant to both drought and rice blast to be listed Marking, select 5 individual plants with listed markers and similar agronomic and yield traits to 98-14geB at the flowering stage, and backcross 98-14geB/F _2-ABCDE to obtain BC ₁ F ₁ ;

5) Plant BC ₁ F ₁ obtained from 4) and self-cross to obtain BC ₁ F ₂ , plant BC ₁ F ₂ in a population of 450 plants, and screen for yellow leaf traits and bare body traits at the three-leaf one-heart to four-leaf stage at the seedling stage. Select seedlings with both yellow leaves and bare body traits to transplant to the same field, and carry out screening tests for resistance to drought and rice blast. Select seedlings that are resistant to drought and rice blast to be marked, and the flowering stage is selected to be marked, and the agronomic and yield traits are the same as those of 98 5 individual plants similar to -14geB were backcrossed with 98-14geB to obtain BC ₂ F ₁ ;

6) Continue to backcross to BC ₄ F ₁ according to the procedure in step 5), and self-cross to obtain BC ₄ F ₂ generations, plant BC ₄ F ₂ generations, and plant the giant embryo male sterile line 98-14geA corresponding to 98-14geB at the same time, seedlings In the _second generation of BC ₄ F, the yellow leaf traits, naked traits, drought resistance, and rice blast resistance were screened (according to the method in step 5), and the rice seedlings with yellow leaf and naked traits, drought resistance, and rice blast resistance were listed and marked. At the flowering stage, select 5 individual plants with excellent agronomic characteristics and the most similarity to 98-14geB among the listed rice plants, and then they can be established as the high-resistant light-bodied yellow-leaf giant embryo rice maintainer line "Anti-Huangguang 98-14geB";

7) "Anti-Yellow Light 98-14geB" was crossed with the giant embryo male sterile line 98-14geA corresponding to 98-14geB and backcrossed for 3 generations, and a three-line male sterile line "Anti Yellow Light 98-14geA".

The three-line CMS line "Kanghuangguang 98-14geA" with high resistance to naked yellow leaf giant embryo rice has the characteristics of giant embryo traits, naked traits, yellow leaf traits, drought resistance, and rice blast resistance. It is used to prepare three-line giant embryo The hybrid rice "Anti-Huangguang 98-14geA/MhgeR" is resistant to drought and rice blast, is easy to remove hybrids, improves the purity of hybrids, and is easy to mechanized harvesting. MhgeR" variety increased yield by 18%.

The above descriptions are only preferred embodiments of the present invention, and all equivalent changes and modifications made according to the scope of the patent application of the present invention shall fall within the scope of the present invention.

Claims (1)

1. a selection for high resistance naked body yellow leaf Characters of Giant Embryo Rice CMS line, is characterized in that: described selection comprises the following steps:

1) select yellow leaf marker parent A, drought-resistant variety B, naked body rice parent C, blast resisting parent D be resistant parent, Characters of Giant Embryo Rice three is maintenance is recurrent parent E, makes hybridization A/B and C/D, must hybridize F ₁in generation, respectively with F _1-ABand F _1-CDrepresent;

2) F is planted _1-ABand F _1-CD, make hybridization F in flowering stage _1-AB/ F _1-CD, must reestablish diplomatic relations F _1-ABCD;

3) F is planted _1-ABCD, selfing obtains F _2-ABCD, plantation F _2-ABCDwith recurrent parent E, F _2-ABCDcolony is in 900-1000 strain, yellow leaf proterties, naked body character screening is carried out in leaf wholeheartedly to the four leaf stage in seedling stage three, select the seedling transplantation simultaneously with Huang Ye and naked body proterties to same field, carry out drought-resistant, blast resisting screening test, select the rice shoot listing mark of simultaneously drought-resistant and rice blast, select listing mark flowering stage and the individual plant of agronomy and yield traits excellence, make hybridization E/F _2-ABCDobtain F _1-ABCDE;

4) 3 are planted) gained F _1-ABCDE, selfing obtains F _2-ABCDE, by F _2-ABCDEseed shells into brown rice, select the brown rice sowing with giant embryo proterties, colony is in 400-500 strain, yellow leaf proterties, naked body character screening is carried out in leaf wholeheartedly to the four leaf stage in seedling stage three, select the seedling transplantation simultaneously with Huang Ye and naked body proterties to same field, carry out drought-resistant, blast resisting screening test, select the drought-resistant rice shoot listing mark with rice blast simultaneously, select listing mark flowering stage and agronomy and yield traits similar to recurrent parent E individual plant, backcross E/F _2-ABCDEobtain BC ₁f ₁;

5) 4 are planted) gained BC ₁f ₁, selfing obtains BC ₁f ₂, plantation BC ₁f ₂colony is in 400-500 strain, yellow leaf proterties, naked body character screening is carried out in leaf wholeheartedly to the four leaf stage in seedling stage three, select the seedling transplantation simultaneously with Huang Ye and naked body proterties to same field, carry out drought-resistant, blast resisting screening test, select the rice shoot listing mark of simultaneously drought-resistant and rice blast, select listing mark flowering stage and agronomy and yield traits similar to recurrent parent E individual plant, to backcross to obtain BC with recurrent parent E ₂f ₁;

6) continue to be returned to BC by the program of step 5) ₄f ₁, selfing obtains BC ₄f ₂generation, plantation BC ₄f ₂in generation, plant giant-embryo CMS lines corresponding to recurrent parent E, seedling stage is to BC simultaneously ₄f ₂in generation, carries out yellow leaf proterties, naked body proterties, drought-resistant, blast resisting screening respectively according to step 5), listing mark has the rice seedling of yellow leaf proterties, naked body proterties, drought-resistant, blast resisting, selects that economical character is excellent, the individual plant the most similar to recurrent parent E is fixed to high resistance naked body Huang leaf Characters of Giant Embryo Rice maintainer flowering stage in the rice strain of listing mark;

7) the yellow leaf Characters of Giant Embryo Rice maintainer of high resistance naked body and giant-embryo CMS lines are hybridized and 3 generations that backcrossed, and are namely bred as high resistance naked body yellow leaf Characters of Giant Embryo Rice CMS line;

Recurrent parent E is 1813geB, TgeB or 98-14geB, and its corresponding giant-embryo CMS lines is 1813geA, TgeA or 98-14geA;

Yellow leaf marker parent A is topaz B, and drought resisting parent B is green non-irrigated No. 1, and naked body rice parent C is light fragrant B, blast resisting parent D is Wunong 1;

The high resistance naked body be bred as yellow leaf Characters of Giant Embryo Rice CMS line has the characteristic of giant embryo proterties, naked body proterties, yellow leaf proterties, drought-resistant, blast resisting simultaneously.