CN104127192A - Fluid system of flow cytometer and flow cell detecting method - Google Patents
Fluid system of flow cytometer and flow cell detecting method Download PDFInfo
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- CN104127192A CN104127192A CN201310485166.2A CN201310485166A CN104127192A CN 104127192 A CN104127192 A CN 104127192A CN 201310485166 A CN201310485166 A CN 201310485166A CN 104127192 A CN104127192 A CN 104127192A
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Abstract
The invention discloses a fluid system of a flow cytometer and a flow cell detecting method. The fluid system of the flow cytometer comprises a sheath flow injector, a sample flow injector, a blood sampling injector, a hemolytic agent injector, a blood sampling needle, a reaction tank, a flowing chamber, a diluent barrel, a hemolytic agent bottle, a first motor, a second motor and a third motor, wherein the sheath flow injector is driven by the first motor; the hemolytic agent injector is driven by the third motor; and the sample flow injector and the blood sampling injector are driven by the second motor. The sample flow injector and the blood sampling injector share the second motor, and are arranged in a moment balancing manner; the second motor is arranged between the two injectors; the cost of the system is reduced; the whole rigidity and the inertia of the system are also improved; sample flow is pushed by the injector stably; and the detection accuracy is improved.
Description
Technical field
The present invention relates to biomedical detection field, relate in particular to a kind of flow cytometer fluid system and fluidic cell detection method.
Background technology
Flow cytometer is one by one by the hemanalysis instrument of detection zone by flow cytometry control cell, flow cytometry refers to that stream of cells (also referred to as sample flow) is under the parcel of sheath fluid, cell is queued up by the fluid control technology of flow chamber detection zone, this technology can avoid cell to cause the problem that detects misalignment through detection zone by irregular route, can greatly reduce cell overlap and produce undetected probability by detection zone simultaneously.
Existing flow cytometry is mainly divided into two large classes, and the first kind is that sheath stream and sample flow all push away sample with syringe, and Equations of The Second Kind is that sample flow is used syringe to push away sample, and sheath stream uses constant voltage source of the gas to push away sample.Constant voltage source of the gas drives sheath stream to need a set of constant voltage air-channel system in addition, and cost is very high.
The fluid scheme of first kind flow cytometry (being that sheath stream and sample flow all push away sample with syringe) as shown in Figure 1, this fluid system is by sheath multiple abscess emitter 5 ', sample flow syringe 13 ', injector for collecting blood 17 ', hemolytic agent syringe 22 ', blood taking needle 19 ', reaction tank 20 ', flow chamber 10 ', diluent bucket 1 ', hemolytic agent bottle 24 ', three-way valve 2 ', two-way valve 3 ', two-way valve 8 ', two-way valve 11 ', two-way valve 18 ', three-way valve 23 ', three way cock 6 ', three way cock 7 ', three way cock 9 ', three way cock 14 ', three way cock 15 ', diluent entrance 30 ', the connected pipeline composition used of flow chamber waste liquid outlet 31 ' and each device.Its mesotheca multiple abscess emitter 5 ' is driven by motor 21 ' by motor 16 ' driving, hemolytic agent syringe 22 ' by motor 12 ' driving, injector for collecting blood 17 ' by motor 4 ' driving, sample flow syringe 13 '.
The workflow of first kind flow cytometer is as follows:
1) take a blood sample by injector for collecting blood 17 ' and blood taking needle 19 ';
2) the rear blood taking needle 19 ' of having taken a blood sample enters reaction tank 20 ', then separates specified blood sample and enters reaction tank 20;
3) adding hemolytic agent by hemolytic agent syringe 22 ' and three-way valve 23 ' enters reaction tank 20 and reacts with blood sample;
4) after blood sample has reacted with hemolytic agent, by sheath multiple abscess emitter 5 ' and two-way valve 8 ' (closing three-way valve 2 ') by blood sample sucking-off reaction tank;
5) sheath multiple abscess emitter 5 pushes away sample together with sample flow syringe 13 ', first open two-way valve 11 ' and three-way valve 2 ', close two-way valve 18 ', sheath multiple abscess emitter 5 ' can be assisted and be pushed away blood sample and enter fast flow chamber 10 ', then blood sample is pushed the detection zone of flow chamber 10 ' again by sample flow syringe 13 ', now open three-way valve 2 ' and two-way valve 3 ', diluent pushes flow chamber 10 ' by sheath multiple abscess emitter 5 ' and forms sheath stream, thereby the queuing of parcel blood sample is carried out hemocyte detection by flow chamber 10 '.
Like this, each syringe of first kind flow cytometer needs to join separately a motor and drives, because the holistic cost of motor and supporting drive transmission is higher, and the therefore high expensive of this scheme.And flow cytometer is high to the stability requirement of sample flow, therefore the rigidity to syringe and inertia requirement are high, batch difficult quality control of material.
Equations of The Second Kind flow cytometer is owing to will adding a set of constant voltage air-channel system, cost costliness, and simultaneity factor complexity is high, and inconvenience is safeguarded.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of flow cytometer fluid system and fluidic cell detection method, the cost that is intended to solve existing flow cytometer is high, and inconvenience safeguards, and sample flow is the problem such as influenced easily.
Technical scheme of the present invention is as follows:
A kind of flow cytometer fluid system, comprises sheath multiple abscess emitter, sample flow syringe, injector for collecting blood, hemolytic agent syringe, blood taking needle, reaction tank, flow chamber, diluent bucket, hemolytic agent bottle, the first motor, the second motor, the 3rd motor, the first three-way valve, the first two-way valve, the second two-way valve, the 4th two-way valve, the second three-way valve; On described flow chamber, be provided with sheath stream input port, sample flow input port and flow chamber waste liquid outlet; The dispensing end of sheath multiple abscess emitter is connected with described sample flow input port with described sheath stream input port respectively; Described sheath multiple abscess emitter is divided on the pipeline between described sheath stream input port described the first three-way valve is set, and one end of described the first three-way valve is connected with described diluent bucket; On pipeline between described the first three-way valve and described sheath stream input port, described the first two-way valve is set; On pipeline between described sheath multiple abscess emitter and described sample flow input port, described the second two-way valve is set; The dispensing end of sample flow syringe is connected with reative cell with described sample flow input port respectively; Described sample flow input port is communicated with the pipeline from described sample flow syringe and described sheath multiple abscess emitter; On the pipeline that described sample flow syringe is connected with reative cell, be provided with described the 4th two-way valve; Described injector for collecting blood is connected with blood taking needle, and described diluent entrance is arranged on described injector for collecting blood; The dispensing end of hemolytic agent syringe arranges described the second three-way valve, is connected respectively with reaction tank with hemolytic agent bottle; Described sheath multiple abscess emitter is driven by the first motor, and described hemolytic agent syringe is driven by the 3rd motor, and wherein, described sample flow syringe and injector for collecting blood drive by the second motor.
Described flow cytometer fluid system, wherein, sample flow injection adopts equalising torque to arrange with injector for collecting blood, arranges that distance meets formula L1 × F1=L2 × F2; Wherein, L1 is the distance at the center of sample flow syringe and the second motor, and L2 is the distance at the center of injector for collecting blood and the second motor, and F1 is the promotion resistance of sample flow syringe, the promotion resistance that F2 is injector for collecting blood.
Described flow cytometer fluid system, wherein, is provided with the 3rd three-way valve between injector for collecting blood and blood taking needle on the pipeline being connected, one end of the 3rd three-way valve is cleaned entrance with sampling needle and is connected.
Described flow cytometer fluid system, wherein, the dispensing end of sample flow syringe is connected with described sample flow input port on pipeline and is provided with a bypass, and the pipeline that described bypass is connected with described sample flow input port with described sheath multiple abscess emitter is connected, and the 3rd two-way valve is set in described bypass.
Described flow cytometer fluid system, wherein, described flow cytometer fluid system also comprises the second hemolytic agent syringe, the second hemolytic agent bottle and the 4th three-way valve;
Described the second hemolytic agent syringe is driven by the 3rd motor, and the dispensing end of the second hemolytic agent syringe arranges described four three-way valve, is connected respectively with reaction tank with the second hemolytic agent bottle.
A kind of fluidic cell detection method, wherein, described fluidic cell detection method adopts flow cytometer fluid system as above to carry out, and specifically comprises the following steps:
1) by the pipeline of the 3rd three-way valve connection injector for collecting blood and blood taking needle, the second motor drives injector for collecting blood, takes a blood sample by blood taking needle; Open the second two-way valve and the first three-way valve, sample flow syringe is drawn diluent from diluent bucket simultaneously;
2) the rear blood taking needle of having taken a blood sample enters reaction tank, then separates specified blood sample and enters reaction tank;
3) the 3rd motor drives hemolytic agent syringe, from hemolytic agent bottle, draws hemolytic agent, then connects the pipeline of hemolytic agent syringe and reaction tank by described the second three-way valve, and hemolytic agent is added in reaction tank and reacted with blood sample;
4) after blood sample has reacted with hemolytic agent, open the second two-way valve and the 4th two-way valve and close the first three-way valve, the first motor drives sheath multiple abscess emitter, by blood sample sucking-off reaction tank;
5) the second motor drives sample flow syringe, directly pushes away blood sample and push the detection zone of flow chamber; Open the first three-way valve and the first two-way valve, the first motor drives sheath multiple abscess emitter, and diluent pushes flow chamber by sheath multiple abscess emitter and forms sheath stream, thereby the queuing of parcel blood sample is carried out blood cell analysis detection by flow chamber.
A kind of fluidic cell detection method, wherein, described fluidic cell detection method adopts flow cytometer fluid system as above to carry out, and specifically comprises the following steps:
1) by the pipeline of the 3rd three-way valve connection injector for collecting blood and blood taking needle, the second motor drives injector for collecting blood, takes a blood sample by blood taking needle; Open the 3rd two-way valve and the first three-way valve, sample flow syringe is drawn diluent from diluent bucket simultaneously;
2) after having taken a blood sample, blood taking needle enters reaction tank, separates specified blood sample and enters reaction tank;
3) the 3rd motor drives hemolytic agent syringe, from hemolytic agent bottle, draws hemolytic agent, then connects the pipeline of hemolytic agent syringe and reaction tank by described the second three-way valve, and hemolytic agent is added in reaction tank and reacted with blood sample;
4) after blood sample has reacted with hemolytic agent, open the second two-way valve and the 4th two-way valve and close the first three-way valve, the first motor drives sheath multiple abscess emitter, by blood sample sucking-off reaction tank;
5) open the 3rd two-way valve and the first three-way valve, close the 4th two-way valve, the first motor drives sheath multiple abscess emitter, and the second motor drives sample flow syringe, blood sample is pushed flow chamber by sheath multiple abscess emitter and sample flow syringe, and then blood sample is pushed the detection zone of flow chamber 1 again by sample flow syringe; Open the first three-way valve and the first two-way valve, the first motor drives sheath multiple abscess emitter, and diluent pushes flow chamber by sheath multiple abscess emitter and forms sheath stream, thereby the queuing of parcel blood sample is carried out blood cell analysis detection by flow chamber.
Described fluidic cell detection method, wherein, in step 5), in the time of the second motor action, sampling syringe links together with sample flow syringe, the 3rd three-way valve is connected the pipeline of sampling syringe and sampling needle cleaning entrance, and sampling syringe pushes away diluent to sampling needle and cleans entrance to clean sampling needle.
Described fluidic cell detection method, wherein, described flow cytometer fluid system also comprises the second hemolytic agent syringe, the second hemolytic agent bottle and the 4th three-way valve; Described the second hemolytic agent syringe is driven by the 3rd motor, and the dispensing end of the second hemolytic agent syringe arranges described four three-way valve, is connected respectively with reaction tank with the second hemolytic agent bottle; Completing steps 3) after, further comprising the steps of:
The 3rd motor drives the second hemolytic agent syringe, from the second hemolytic agent bottle, draw the second hemolytic agent, then the pipeline of connecting the second hemolytic agent syringe and reaction tank by described the 4th three-way valve, adds the second hemolytic agent in reaction tank to and reacts with blood sample.
Flow cytometer fluid system provided by the present invention, the drive motors of sample flow syringe and injector for collecting blood is merged into one, these two syringes adopt equalising torque to arrange simultaneously, and motor arrangement is between these two syringes, and its beneficial effect is: 1) reduce system cost; 2) after two syringe common motor, integral rigidity and inertia increase, and it is more steady that syringe pushes away sample flow, improve detection accuracy; 3) two syringe equalising torques layout rear drive resistances can be cancelled out each other, and can improve the service life of drive motors and syringe.
Brief description of the drawings
Fig. 1 is the structural representation of first kind flow cytometer in prior art.
Fig. 2 is the structural representation of flow cytometer fluid system provided by the present invention.
Fig. 3 is the structural representation that in the present invention, sample flow syringe and injector for collecting blood equalising torque are arranged.
Fig. 4 is the structural representation of flow cytometer fluid system in the embodiment of the present invention 1.
Fig. 5 is the structural representation of flow cytometer fluid system in the embodiment of the present invention 2.
Fig. 6 is the structural representation of flow cytometer fluid system in the embodiment of the present invention 3.
Detailed description of the invention
The invention provides a kind of flow cytometer fluid system and fluidic cell detection method, for making object of the present invention, technical scheme and effect clearer, clear and definite, below the present invention is described in more detail.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
A kind of flow cytometer fluid system provided by the present invention, it mainly improves and is, by the drive motors of sample flow syringe and injector for collecting blood is merged into one, has reduced a set of motor and drive transmission, can reduce costs, and can improve the stationarity of sample flow.Owing to just merging drive motors (this two syringe moves few in flow cytometry analysis process, the syringe action of arranging of can staggering) for sample flow syringe and injector for collecting blood, therefore overall measurement speed is unaffected simultaneously.
Particularly, described flow cytometer fluid system, as shown in Figure 2, comprise sheath multiple abscess emitter 5, sample flow syringe 13, injector for collecting blood 17, hemolytic agent syringe 22, blood taking needle 19, reaction tank 20, flow chamber 10, diluent bucket 1, hemolytic agent bottle 24, the first motor 4, the second motor 12, the 3rd motor 21, the first three-way valve 2, the first two-way valve 3, the second two-way valve 8, the 3rd two-way valve 11, the 4th two-way valve 18, the second three-way valve 23, the 3rd three-way valve 25, the first three way cock 6, the second three way cock 7, the 3rd three way cock 9, the 4th three way cock 14, the 5th three way cock 15 and the each device pipeline used that is connected, described the first three way cock 6, the second three way cock 7, the 3rd three way cock 9, the 4th three way cock 14, the 5th three way cock 15, for making pipeline generate bypass, are connected corresponding parts,
Described sheath multiple abscess emitter 5 is connected with described the first motor 4, is driven by the first motor 4; Described sample flow syringe 13 is connected with the second motor 12 respectively with injector for collecting blood 17, drives by the second motor 12; Described hemolytic agent syringe 22 is connected with the 3rd motor 21, is driven by the 3rd motor 21;
On described flow chamber 10, be provided with sheath stream input port, sample flow input port and flow chamber waste liquid outlet 31;
The dispensing end of described sheath multiple abscess emitter 5 is divided into twice pipeline through described the first three way cock 6, is connected respectively with the sheath stream input port of flow chamber 10 and the sample flow input port of flow chamber 10; On pipeline between the sheath stream input port of described sheath multiple abscess emitter 5 and flow chamber 10, described the first three-way valve 2 is set, one end (Chang Kaiduan) of described the first three-way valve 2 is connected with described diluent bucket 1; On pipeline between the sheath stream input port of described the first three-way valve 2 and flow chamber 10, described the first two-way valve 3 is also set; Described the second two-way valve 8 is set on the pipeline between described sheath multiple abscess emitter 5 and the sample flow input port of flow chamber 10;
The dispensing end of described sample flow syringe 13 is divided into twice pipeline through described the 5th three way cock 15, is connected respectively with the sample flow input port of described flow chamber 10 with reative cell 20; The sample flow input port place of described flow chamber 10 arranges described the 3rd three way cock 9, is communicated with the pipeline with described sheath multiple abscess emitter 5 from described sample flow syringe 13; On the pipeline that described sample flow syringe 13 is connected with reative cell 20, be provided with described the 4th two-way valve 18;
Described injector for collecting blood 17 is connected with blood taking needle 19, and described diluent entrance 30 is arranged on described injector for collecting blood 17;
The dispensing end of described hemolytic agent syringe 22 arranges described the second three-way valve 23, is connected respectively (Chang Kaiduan of described the second three-way valve 23 is connected with described solvent blood bottle 24) with reaction tank 20 with hemolytic agent bottle 24.
As shown in Figure 3, sample flow syringe 13 and injector for collecting blood 17 are carried out to equalising torque layout, the distance at sample flow syringe 13 and the second motor 12 center is L1, the distance at injector for collecting blood 17 and the second motor 12 center is L2, the promotion resistance of sample flow syringe 13 is F1, and the promotion resistance of syringe 17 is F2, and layout distance L 1 and the L2 of syringe meet formula: L1 × F1=L2 × F2, meet equalising torque, therefore can improve and drive stationarity and motor service life.Due to the existence of two syringes, integral rigidity and inertia increase, and can cushion not stationarity of motor-driven gap simultaneously, obtain than driving single syringe scheme sample flow more stably, thereby improve the accuracy of detection of fluidic cell.
In the present invention, in order to reduce costs, sample flow syringe 13 shares a motor with injector for collecting blood 17, in the time that sample flow syringe 13 moves, affect the blood sample in blood taking needle 19, described the 3rd three-way valve 25 can be set on the pipeline that described injector for collecting blood 17 is connected with blood taking needle 19.The Chang Kaiduan of described the 3rd three-way valve 25 connects sampling needle and cleans entrance 29, only take a blood sample by injector for collecting blood 17 at needs and divide blood in just open described the 3rd three-way valve 25, described in other moment, the 3rd three-way valve 25, in closed condition, is connected described injector for collecting blood 17 and sampling needle and is cleaned entrance 29.In the time that the second motor 12 moves, close the 3rd three-way valve 25, just can so just can eliminate sample flow syringe 13 and move time, the interior blood sample of blood taking needle 19 is exerted an influence.
On the pipeline that is connected between the sample flow input port of the dispensing end of described sample flow syringe 13 and described flow chamber 10, can also be provided with a bypass, the pipeline that described bypass is connected with the sample flow input port of described flow chamber 10 with described sheath multiple abscess emitter 5 is connected, and described the 3rd two-way valve 11 is set in described bypass.Particularly, the above the 4th three way cock 14 of pipeline between described sample flow syringe 13 and described the 5th three way cock 15, generate described bypass, accordingly, described sheath multiple abscess emitter 5 is provided with the second three way cock 7 with corresponding on the connected pipeline in the sample flow input port of described flow chamber 10, is connected with described bypass.Like this, in the time that sample flow syringe 13 promotes blood sample and enters flow chamber 10, open described the second two-way valve 11, described sheath multiple abscess emitter 5 just can be assisted blood sample is pushed to flow chamber 10 fast.
A kind of fluidic cell detection method is also provided in the present invention, and described method adopts described flow cytometer fluid system to carry out, and its workflow is as follows:
1) open the 3rd three-way valve 25, the second motors 12 and drive injector for collecting blood 17, take a blood sample by blood taking needle 19; Open the 3rd two-way valve 11 and the first three-way valve 2, sample flow syringe 13 is drawn diluent from diluent bucket 1 simultaneously; (open the 3rd three-way valve 25, the 3rd two-way valve 11 and the first three-way valve 2 simultaneously, sample flow syringe 13 and injector for collecting blood 17 worked simultaneously, the impact on sample flow syringe 13 and reaction tank 20 interior liquid can eliminate the second motor 12 and move time);
2) after having taken a blood sample, blood taking needle 19 enters reaction tank 20, separates specified blood sample and enters reaction tank 20;
3) the 3rd motor 21 drives hemolytic agent syringe 22, from hemolytic agent bottle 24, draws hemolytic agent, then opens described the second three-way valve 23, and hemolytic agent is added in reaction tank 20 and reacted with blood sample;
4) after blood sample has reacted with hemolytic agent, open the second two-way valve 8 and the 4th two-way valve 18 and close the first three-way valve 2, the first motors 4 and drive sheath multiple abscess emitter 5, by blood sample sucking-off reaction tank 20;
5) open the 3rd two-way valve 11 and the first three-way valve 2, close the 4th two-way valve 18, the first motor 4 drives sheath multiple abscess emitter 5, the second motor 12 drives sample flow syringe 13, blood sample is pushed flow chamber 10 by sheath multiple abscess emitter 5 and sample flow syringe 13, and then blood sample is pushed the detection zone of flow chamber 10 again by sample flow syringe 13; Open the first three-way valve 2 and the first two-way valve 3, the first motors 4 drive sheath multiple abscess emitter 5, diluent pushes flow chamber 10 by sheath multiple abscess emitter 5 and forms sheath and flow, thereby parcel blood sample is queued up and carried out blood cell analysis detection by flow chamber 10.
Wherein, in step 5), in the time that the second motor 12 moves, sampling syringe 17 links together with sample flow syringe 13, and the 3rd three-way valve 25 is in closed condition, and sampling syringe 17 pushes away diluent to sampling needle and cleans entrance 29 to clean sampling needle.
Owing to reducing by a motor, simultaneously the drive transmission of motor also reduces a set ofly, and increases cost that a three-way valve the brings cost far below a set of motor, and therefore technical scheme cost provided by the present invention greatly reduces.Meanwhile, sample flow syringe and injector for collecting blood adopt equalising torque to arrange, integral rigidity and inertia increase, and not stationarity of the gap of available buffer drive motors, can obtain sample flow more stably, thereby improve the accuracy of detection of flow cytometer.In addition, sample flow syringe and injector for collecting blood are merged into after same motor driving, and two motor action times were staggered and arranged, and also do not affect the measuring speed of flow cytometer.
The present invention will be further described by the following examples.
Embodiment 1:
As shown in Figure 4, a kind of composition of flow cytometer fluid system comprises sheath multiple abscess emitter 5, sample flow syringe 13, injector for collecting blood 17, hemolytic agent syringe 22, blood taking needle 19, reaction tank 20, flow chamber 10, diluent bucket 1, hemolytic agent bottle 24, the first three-way valve 2, the first two-way valve 3, the second two-way valve 8, the 4th two-way valve 18, the second three-way valve 23, the 3rd three-way valve 25, the first three way cock 6, the 4th three way cock 14, the 5th three way cock 15 and the each device pipeline used that is connected.29 for sampling needle cleans entrance, and 30 is diluent entrance, and 31 is flow chamber waste liquid outlet.Wherein, sheath multiple abscess emitter 5 by the first motor 4 drive, sample flow syringe 13 and injector for collecting blood 17 by the second motor 12 drive, hemolytic agent syringe 22 drives by the 3rd motor 21.Sample flow syringe 13 adopts equalising torque to arrange with injector for collecting blood 17, and the second motor 12 is arranged between two syringes.
The difference of the scheme in this scheme and Fig. 2 has been to reduce the bypass at the 3rd two-way valve 11 places, without the auxiliary sample flow branch road that pushes away.
This scheme workflow is as follows:
1) open the 3rd three-way valve 25, the second motors 12 and drive injector for collecting blood 17, take a blood sample by blood taking needle 19; Open the second two-way valve 8 and the first three-way valve 2, sample flow syringe 13 is drawn diluent from diluent bucket 1 simultaneously; (impacts on sample flow syringe 13 and reaction tank 20 interior liquid when elimination motor 12 moves);
2) the rear blood taking needle 19 of having taken a blood sample enters reaction tank 20, then separates specified blood sample and enters reaction tank 20;
3) the 3rd motor 21 drives hemolytic agent syringe 22, from hemolytic agent bottle 24, draws hemolytic agent, then opens described the second three-way valve 23, and hemolytic agent is added in reaction tank 20 and reacted with blood sample;
4) after blood sample has reacted with hemolytic agent, open the second two-way valve 8 and the 4th two-way valve 18 and close the first three-way valve 2, the first motors 4 and drive sheath multiple abscess emitter 5, by blood sample sucking-off reaction tank 20;
5) the second motor 12 drives sample flow syringe 13, (the second motor 12 moves directly to push away the detection zone that blood sample pushes flow chamber 10, sampling syringe 17 links together with sample flow syringe 13, the 3rd three-way valve 25 is not opened, and sampling syringe 17 pushes away diluent to sampling needle and cleans entrance 29 to clean sampling needle); Open the first three-way valve 2 and the first two-way valve 3, the first motors 4 drive sheath multiple abscess emitter 5, diluent pushes flow chamber 10 by sheath multiple abscess emitter 5 and forms sheath and flow, thereby parcel blood sample is queued up and carried out blood cell analysis detection by flow chamber 10.
Embodiment 2:
As shown in Figure 5, a kind of composition of flow cytometer fluid system comprises sheath multiple abscess emitter 5, sample flow syringe 13, injector for collecting blood 17, hemolytic agent syringe 22, the second hemolytic agent syringe 26, blood taking needle 19, reaction tank 20, flow chamber 10, diluent bucket 1, hemolytic agent bottle 24, the second hemolytic agent bottle 28, the first three-way valve 2, the first two-way valve 3, the second two-way valve 8, the 4th two-way valve 18, the second three-way valve 23, the 3rd three-way valve 25, the 4th three-way valve 27, the first three way cock 6, the 4th three way cock 14, the 5th three way cock 15 and the each device pipeline used that is connected.29 for sampling needle cleans entrance, and 30 is diluent entrance, and 31 is flow chamber waste liquid outlet.Wherein, sheath multiple abscess emitter 5 by the first motor 4 drive, sample flow syringe 13 and injector for collecting blood 17 by the second motor 12 drive, hemolytic agent syringe 22 and the second hemolytic agent syringe 26 drive by the 3rd motor 21.Sample flow syringe 13 and injector for collecting blood 17 equalising torques layouts, the second motor 12 is arranged between two syringes.
This scheme workflow:
1) open the 3rd three-way valve 25, the second motors 12 and drive injector for collecting blood 17, take a blood sample by blood taking needle 19; Open the second two-way valve 8 and the first three-way valve 2, sample flow syringe 13 is drawn diluent from diluent bucket 1 simultaneously; (impacts on sample flow syringe 13 and reaction tank 20 interior liquid when elimination motor 12 moves);
2) the rear blood taking needle 19 of having taken a blood sample enters reaction tank 20, then separates specified blood sample and enters reaction tank 20;
3) the 3rd motor 21 drives hemolytic agent syringe 22, from hemolytic agent bottle 24, draws hemolytic agent, then opens described the second three-way valve 23, and hemolytic agent is added in reaction tank 20 and reacted with blood sample;
4) the 3rd motor 21 drives the second hemolytic agent syringe 26, from the second hemolytic agent bottle 28, draws the second hemolytic agent, then opens described the 4th three-way valve 27, and the second hemolytic agent is added in reaction tank 20 and reacted with blood sample;
5) after blood sample has reacted with hemolytic agent, open the second two-way valve 8 and the 4th two-way valve 18 and close the first three-way valve 2, the first motors 4 and drive sheath multiple abscess emitter 5, by blood sample sucking-off reaction tank 20;
6) the second motor 12 drives sample flow syringe 13, (the second motor 12 moves directly to push away the detection zone that blood sample pushes flow chamber 10, sampling syringe 17 links together with sample flow syringe 13, the 3rd three-way valve 25 is not opened, and sampling syringe 17 pushes away diluent to sampling needle and cleans entrance 29 to clean sampling needle); Open the first three-way valve 2 and the first two-way valve 3, the first motors 4 drive sheath multiple abscess emitter 5, diluent pushes flow chamber 10 by sheath multiple abscess emitter 5 and forms sheath and flow, thereby parcel blood sample is queued up and carried out blood cell analysis detection by flow chamber 10.
Embodiment 2 is with the difference of embodiment 1, for strengthening the reaction effect of blood sample and hemolytic agent, increases a kind of hemolytic agent, therefore, has increased the second hemolytic agent syringe 26, the 4th three-way valve 27 and the second hemolytic agent bottle 28 in the present embodiment scheme.
Embodiment 3
As shown in Figure 6, a kind of composition of flow cytometer fluid system comprises sheath multiple abscess emitter 5, sample flow syringe 13, injector for collecting blood 17, hemolytic agent syringe 22, the second hemolytic agent syringe 26, blood taking needle 19, reaction tank 20, flow chamber 10, diluent bucket 1, hemolytic agent bottle 24, the second hemolytic agent bottle 28, the first three-way valve 2, the first two-way valve 3, the second two-way valve 8, the 3rd two-way valve 11, the 4th two-way valve 18, the second three-way valve 23, the 3rd three-way valve 25, the 4th three-way valve 27, the first three way cock 6, the second three way cock 7, the 3rd three way cock 9, the 4th three way cock 14, the 5th three way cock 15 and the each device pipeline used that is connected.29 for sampling needle cleans entrance, and 30 is diluent entrance, and 31 is flow chamber waste liquid outlet.Wherein, wherein, sheath multiple abscess emitter 5 by the first motor 4 drive, sample flow syringe 13 and injector for collecting blood 17 by the second motor 12 drive, hemolytic agent syringe 22 and the second hemolytic agent syringe 26 drive by the 3rd motor 21.Sample flow syringe 13 and injector for collecting blood 17 equalising torques layouts, the second motor 12 is arranged between two syringes.
This scheme workflow:
1) open the 3rd three-way valve 25, the second motors 12 and drive injector for collecting blood 17, take a blood sample by blood taking needle 19; Open the 3rd two-way valve 11 and the first three-way valve 2, sample flow syringe 13 is drawn diluent from diluent bucket 1 simultaneously; (impacts on sample flow syringe 13 and reaction tank 20 interior liquid when elimination the second motor 12 moves);
2) after having taken a blood sample, blood taking needle 19 enters reaction tank 20, separates specified blood sample and enters reaction tank 20;
3) the 3rd motor 21 drives hemolytic agent syringe 22, from hemolytic agent bottle 24, draws hemolytic agent, then opens described the second three-way valve 23, and hemolytic agent is added in reaction tank 20 and reacted with blood sample;
4) the 3rd motor 21 drives the second hemolytic agent syringe 26, from the second hemolytic agent bottle 28, draws the second hemolytic agent, then opens described the 4th three-way valve 27, and the second hemolytic agent is added in reaction tank 20 and reacted with blood sample;
5) after blood sample has reacted with hemolytic agent, open the second two-way valve 8 and the 4th two-way valve 18 and close the first three-way valve 2, the first motors 4 and drive sheath multiple abscess emitter 5, by blood sample sucking-off reaction tank 20;
6) open the 3rd two-way valve 11 and the first three-way valve 2, close the 4th two-way valve 18, the first motor 4 drives sheath multiple abscess emitter 5, the second motor 12 drives sample flow syringe 13, sheath multiple abscess emitter 5 and sample flow syringe 13 push blood sample flow chamber 10 jointly, then blood sample pushes the detection zone of flow chamber 10 by sample flow syringe 13 again (the second motor 12 moves, sampling syringe 17 links together with sample flow syringe 13, the 3rd three-way valve 25 is not opened, and sampling syringe 17 pushes away diluent to sampling needle and cleans entrance 29 to clean sampling needle); Open the first three-way valve 2 and the first two-way valve 3, the first motors 4 drive sheath multiple abscess emitter 5, diluent pushes flow chamber 10 by sheath multiple abscess emitter 5 and forms sheath and flow, thereby parcel blood sample is queued up and carried out blood cell analysis detection by flow chamber 10.
Embodiment 3 is with the difference of embodiment 2, has increased the bypass at the 3rd two-way valve 11 places, increases to assist to push away sample flow branch road fast blood sample is pushed to flow chamber 10.
Above three embodiment merge into one by the drive motors of sample flow syringe and injector for collecting blood, and these two syringes adopt equalising torque to arrange simultaneously, and motor arrangement is between these two syringes, and its beneficial effect is: 1) reduce system cost; 2) after two syringe common motor, integral rigidity and inertia increase, and it is more steady that syringe pushes away sample flow, improve detection accuracy; 3) two syringe equalising torques layout rear drive resistances can be cancelled out each other, and can improve the service life of drive motors and syringe.
Should be understood that, application of the present invention is not limited to above-mentioned giving an example, and for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (9)
1. a flow cytometer fluid system, comprises sheath multiple abscess emitter, sample flow syringe, injector for collecting blood, hemolytic agent syringe, blood taking needle, reaction tank, flow chamber, diluent bucket, hemolytic agent bottle, the first motor, the second motor, the 3rd motor, the first three-way valve, the first two-way valve, the second two-way valve, the 4th two-way valve, the second three-way valve; On described flow chamber, be provided with sheath stream input port, sample flow input port and flow chamber waste liquid outlet; The dispensing end of sheath multiple abscess emitter is connected with described sample flow input port with described sheath stream input port respectively; Described sheath multiple abscess emitter is divided on the pipeline between described sheath stream input port described the first three-way valve is set, and one end of described the first three-way valve is connected with described diluent bucket; On pipeline between described the first three-way valve and described sheath stream input port, described the first two-way valve is set; On pipeline between described sheath multiple abscess emitter and described sample flow input port, described the second two-way valve is set; The dispensing end of sample flow syringe is connected with reative cell with described sample flow input port respectively; Described sample flow input port is communicated with the pipeline from described sample flow syringe and described sheath multiple abscess emitter; On the pipeline that described sample flow syringe is connected with reative cell, be provided with described the 4th two-way valve; Described injector for collecting blood is connected with blood taking needle, and described diluent entrance is arranged on described injector for collecting blood; The dispensing end of hemolytic agent syringe arranges described the second three-way valve, is connected respectively with reaction tank with hemolytic agent bottle; Described sheath multiple abscess emitter is driven by the first motor, and described hemolytic agent syringe is driven by the 3rd motor, it is characterized in that, described sample flow syringe and injector for collecting blood drive by the second motor.
2. flow cytometer fluid system according to claim 1, is characterized in that, sample flow injection adopts equalising torque to arrange with injector for collecting blood, arranges that distance meets formula L1 × F1=L2 × F2; Wherein, L1 is the distance at the center of sample flow syringe and the second motor, and L2 is the distance at the center of injector for collecting blood and the second motor, and F1 is the promotion resistance of sample flow syringe, the promotion resistance that F2 is injector for collecting blood.
3. flow cytometer fluid system according to claim 1, is characterized in that, is provided with the 3rd three-way valve between injector for collecting blood and blood taking needle on the pipeline being connected, and one end of the 3rd three-way valve is cleaned entrance with sampling needle and is connected.
4. flow cytometer fluid system according to claim 1, it is characterized in that, the dispensing end of sample flow syringe is connected with described sample flow input port on pipeline and is provided with a bypass, the pipeline that described bypass is connected with described sample flow input port with described sheath multiple abscess emitter is connected, and the 3rd two-way valve is set in described bypass.
5. flow cytometer fluid system according to claim 1, is characterized in that, described flow cytometer fluid system also comprises the second hemolytic agent syringe, the second hemolytic agent bottle and the 4th three-way valve;
Described the second hemolytic agent syringe is driven by the 3rd motor, and the dispensing end of the second hemolytic agent syringe arranges described four three-way valve, is connected respectively with reaction tank with the second hemolytic agent bottle.
6. a fluidic cell detection method, is characterized in that, described fluidic cell detection method adopts flow cytometer fluid system as claimed in claim 1 to carry out, and specifically comprises the following steps:
1) by the pipeline of the 3rd three-way valve connection injector for collecting blood and blood taking needle, the second motor drives injector for collecting blood, takes a blood sample by blood taking needle; Open the second two-way valve and the first three-way valve, sample flow syringe is drawn diluent from diluent bucket simultaneously;
2) the rear blood taking needle of having taken a blood sample enters reaction tank, then separates specified blood sample and enters reaction tank;
3) the 3rd motor drives hemolytic agent syringe, from hemolytic agent bottle, draws hemolytic agent, then connects the pipeline of hemolytic agent syringe and reaction tank by described the second three-way valve, and hemolytic agent is added in reaction tank and reacted with blood sample;
4) after blood sample has reacted with hemolytic agent, open the second two-way valve and the 4th two-way valve and close the first three-way valve, the first motor drives sheath multiple abscess emitter, by blood sample sucking-off reaction tank;
5) the second motor drives sample flow syringe, directly pushes away blood sample and push the detection zone of flow chamber; Open the first three-way valve and the first two-way valve, the first motor drives sheath multiple abscess emitter, and diluent pushes flow chamber by sheath multiple abscess emitter and forms sheath stream, thereby the queuing of parcel blood sample is carried out blood cell analysis detection by flow chamber.
7. a fluidic cell detection method, is characterized in that, described fluidic cell detection method adopts flow cytometer fluid system as claimed in claim 4 to carry out, and specifically comprises the following steps:
1) by the pipeline of the 3rd three-way valve connection injector for collecting blood and blood taking needle, the second motor drives injector for collecting blood, takes a blood sample by blood taking needle; Open the 3rd two-way valve and the first three-way valve, sample flow syringe is drawn diluent from diluent bucket simultaneously;
2) after having taken a blood sample, blood taking needle enters reaction tank, separates specified blood sample and enters reaction tank;
3) the 3rd motor drives hemolytic agent syringe, from hemolytic agent bottle, draws hemolytic agent, then connects the pipeline of hemolytic agent syringe and reaction tank by described the second three-way valve, and hemolytic agent is added in reaction tank and reacted with blood sample;
4) after blood sample has reacted with hemolytic agent, open the second two-way valve and the 4th two-way valve and close the first three-way valve, the first motor drives sheath multiple abscess emitter, by blood sample sucking-off reaction tank;
5) open the 3rd two-way valve and the first three-way valve, close the 4th two-way valve, the first motor drives sheath multiple abscess emitter, and the second motor drives sample flow syringe, blood sample is pushed flow chamber by sheath multiple abscess emitter and sample flow syringe, and then blood sample is pushed the detection zone of flow chamber 1 again by sample flow syringe; Open the first three-way valve and the first two-way valve, the first motor drives sheath multiple abscess emitter, and diluent pushes flow chamber by sheath multiple abscess emitter and forms sheath stream, thereby the queuing of parcel blood sample is carried out blood cell analysis detection by flow chamber.
8. according to the fluidic cell detection method described in claim 6 or 7, it is characterized in that, in step 5), in the time of the second motor action, sampling syringe links together with sample flow syringe, the 3rd three-way valve is connected the pipeline of sampling syringe and sampling needle cleaning entrance, and sampling syringe pushes away diluent to sampling needle and cleans entrance to clean sampling needle.
9. according to the fluidic cell detection method described in claim 6 or 7, it is characterized in that, described flow cytometer fluid system also comprises the second hemolytic agent syringe, the second hemolytic agent bottle and the 4th three-way valve; Described the second hemolytic agent syringe is driven by the 3rd motor, and the dispensing end of the second hemolytic agent syringe arranges described four three-way valve, is connected respectively with reaction tank with the second hemolytic agent bottle; Completing steps 3) after, further comprising the steps of:
The 3rd motor drives the second hemolytic agent syringe, from the second hemolytic agent bottle, draw the second hemolytic agent, then the pipeline of connecting the second hemolytic agent syringe and reaction tank by described the 4th three-way valve, adds the second hemolytic agent in reaction tank to and reacts with blood sample.
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