CN104118836B - The filling device of culture and aseptic filling method is carried out under a kind of aseptic condition - Google Patents

Abstract

Carrying out the filling device of culture under aseptic condition, wherein, comprise for placing the first platform of medium bottle and the electric lifting platform for culture dish holding, and the level height of the first platform is higher than the level height of the second platform; One " n " grass tube, described bottle cap is provided with an admission port, and on described admission port, connection one is with the tracheae of air valve; The top of conduit is clamped by the clip on one second support, and a described Gas Pipe being provided with burner can pass conduit, and described Gas Pipe is connected to source of the gas by gas valve.And provide a kind of aseptic filling method.This apparatus structure is simple, only has medium bottle, conduit and culture dish, can carry out flame sterilization easily to it, can realize absolute aseptic, meet the General Requirements of sterile working.

Description

A device and aseptic filling method for medium filling under aseptic conditions

technical field

The proposal relates to a device and method for aseptically filling petri dishes used in a laboratory, specifically a device and aseptic filling method for medium filling under aseptic conditions.

Background technique

It is one of the basic experimental operations in the laboratory to make "plates" by filling culture dishes with culture medium. A large number of such plates are used in operations such as pathogenic bacteria isolation and resistance identification, which requires a lot of work. The general operation procedure is to take out the sterilized medium from the refrigerator, heat and melt it in a water bath or microwave oven, and then use the flame of an alcohol lamp on the ultra-clean workbench to assist in creating a sterile environment, and then introduce the melted medium into a petri dish , one by one operation, after the culture medium is cooled and solidified, then the related inoculation operation is carried out. The disadvantage of this method is that, firstly, the efficiency is low. The culture medium melts and cools to a certain temperature (not too hot, the bottle can be held in hand, and does not solidify, and the general temperature is above 60°C), and it is time-consuming to manually pour it in one by one. The second is that it is easy to pollute. The key link is that the medium is exposed to the air for a long time when it is dumped. Although it is an ultra-clean workbench, there is still a certain chance of contamination; Achieve quantification. At present, there are similar large-scale medium dispensing instruments on the market, which are a set of equipment that combines medium preparation and medium dispensing. The price of only the dispensing part is more than 300,000 yuan. Suitable for bulk petri dish packing.

This patent aims to invent a simple automatic quantitative filling device for culture medium under aseptic conditions to realize quantitative subpackaging of small batches of culture dishes. The device has a simple structure, low price, and is easy to produce and use.

Contents of the invention

In order to solve the deficiencies of the prior art, the present invention provides a device and aseptic filling method for medium filling under aseptic conditions. Firstly, it is a low-cost filling device, and secondly, it can realize The filling is simple, and it can realize the absolute aseptic filling operation of small batches of medium.

The technical solution adopted by the present invention to solve the technical problem is: a device for filling culture medium under aseptic conditions, comprising

A base that provides support and mounting space;

The first support for placing the culture medium bottle, a height-adjustable first platform is fixed on the first support, a culture medium bottle is placed on the first platform, and the mouth of the culture medium bottle is Conical;

An electric lifting platform for placing culture dishes, the electric lifting platform includes a second platform, a jacking rod, a lead screw, a worm gear and a drive motor, and the second platform is installed on the base through a jacking rod and a linear bearing, And at the lower end of the jacking rod, a coaxial lead screw is fixed, a worm gear is provided with a wire sleeve and fitted on the lead screw, and the drive motor is directly connected with the worm; a petri dish is placed on the second platform seat, a petri dish is placed in the petri dish seat;

the level of the first platform is higher than the level of the second platform;

An "n"-shaped glass conduit, the high point entrance of the conduit is provided with an externally open conical bottle cap, and at the surface where the conical bottle cap and the conical bottle mouth of the culture medium bottle match It is a frosted glass mating surface, an air inlet is provided on the bottle cap, and an air pipe with an air valve is connected to the air inlet;

the top of the conduit is held by a clip on a second bracket,

A gas pipe fitted with a burner can pass through the conduit, and the gas pipe is connected to a gas source through a gas valve.

Further, the gas source is hydrogen, and the auxiliary gas source is oxygen.

The method for aseptic filling based on the above-mentioned device comprises the following steps, characterized in that,

Step 1, the device is placed in the aseptic area of the aseptic operation platform, and the culture medium bottle is placed in the constant temperature barrel of the first platform for use, and the temperature of the constant temperature barrel is sufficient to melt the medium and maintain a liquid state;

Step 2: Put the burner through the conduit and pass it out, open the gas valve and ignite the burner, then fire the bottle cap on the conduit, and pull the gas pipe so that the burner in the burning state retreats from the inside of the conduit. During the process, the sterilization of the inner wall of the catheter is completed, and finally the gas valve is closed and the end of the catheter with the bottle cap is inserted into the culture medium bottle;

Step 3: Use a sterile suction pipe to inhale the catheter and keep the air valve in the trachea open during the suction process until the culture medium flows out of the catheter, withdraw the suction pipe and close the air valve, and use the "siphon function" "Realize the automatic distribution of culture medium;

Step 4, place the culture dish to be filled on the second platform, and then start the driving motor, so that the second platform rises to the liquid outlet of the conduit and inserts it into the culture dish, then opens the air valve, and the culture medium drips into the Finally close the air valve in the petri dish, and then reset the second platform and remove the petri dish;

Step 5, repeat step 4 to complete the filling of multiple culture dishes.

Further, the temperature of the thermostatic barrel is kept above 60°C.

The beneficial effects of the present invention are:

1. The structure is simple, there are only culture medium bottles, catheters and petri dishes, which can be easily flame sterilized, can achieve absolute sterility, and meet the general requirements of aseptic operation.

2. It is operable and has good performance. It can be sterilized by flame method, and can be sterilized by bare hands. The flame temperature is high, which is very convenient compared with burning with alcohol lamp or using high pressure sterilization, and the sterilization effect is better.

3. It can replace expensive imported aseptic filling equipment.

Description of drawings

Figure 1 is a schematic diagram.

Fig. 2 is a schematic perspective view of the device.

Fig. 3 is a schematic perspective view after removing the base.

Fig. 4 is a front view of Fig. 3 .

Figure 5 is a schematic diagram of the filling process.

Fig. 6 is a schematic diagram of the process of burning and sterilizing the burner.

Fig. 7 is a flow chart of the aseptic disinfection method.

In the figure: 1 base, 11 switch and button, 12 second support, 2 medium bottle, 21 first support, 22 first platform, 23 constant temperature barrel, 3 electric lifting platform, 31 jacking rod, 32 second platform, 33 leading screws, 34 worm gears, 35 driving motors, 36 petri dish seats, 4 petri dishes, 5 conduits, 51 conical bottle caps, 52 air inlets, 6 gas pipes, and 61 burners.

Detailed ways

As shown in Figure 1 to Figure 6,

A device for filling culture medium under aseptic conditions consists of the following parts.

A base 1 providing support and installation space, on which switches and buttons 11 are installed.

The first bracket 21 for placing the culture medium bottle 2 is made of stainless steel rods. The lower end of the first bracket is fixed on the base by screws, and a height-adjustable first platform 22 is fixed on the first bracket 21. By fastening The screw can adjust the effective height of the first platform. A culture medium bottle 2 is placed on the first platform 22. The mouth of the culture medium bottle 2 is conical, and the outer surface of the conical bottle mouth is frosted. And place a constant temperature barrel 23 with electric heating function on the first support, the culture medium bottle can be placed in the constant temperature barrel, the internal temperature is kept between 50 degrees and 60 degrees by electric heating, so that the culture medium becomes into a liquid state for filling.

The electric lifting platform 3 that is used to place culture dish, described electric lifting platform comprises second platform 32, jacking rod 31, leading screw 33, worm gear 34 and driving motor 35, and described second platform 32 is passed through jacking rod and The linear bearing is installed on the base 1 and can move up and down. A coaxial lead screw 33 is fixed at the lower end of the jacking rod 31, a worm gear is provided with a thread sleeve and is sleeved on the lead screw, and the drive motor 35 is directly connected with the worm screw, so that under the drive of the drive motor 35 , can drive the second platform to rise or fall to form an electric lifting platform. A petri dish seat 36 is placed on the second platform 32, and there is a socket in the petri dish seat, and then a petri dish 4 is placed in the petri dish seat to realize the fixing of the petri dish.

Among the above-mentioned platforms, the level of the first platform 22 is higher than that of the second platform 32, so that a height difference is formed to facilitate filling. Optimally, both the first platform and the second platform are made of stainless steel plates.

An "n" glass conduit 5, wherein the nozzles at both ends are high and the other is low, and the height difference H is basically similar to the height difference of the two platforms. The high point inlet of conduit 5 is provided with the conical bottle cap 51 of an outer opening, can insert in the culture medium bottle 2 easily like this, and the conical bottle mouth of described conical bottle cap and culture medium bottle Erlenmeyer bottle The matching surface is frosted glass mating surface, forming a good sealed connection. At the same time, an air inlet 52 is provided on the bottle cap, and an air pipe with an air valve is connected to the air inlet, and the flow rate can be controlled by closing and opening the air valve.

The top of the conduit 5 is clamped by a clip on a second bracket 12, which is a bendable metal hose or metal bellows, has a certain degree of flexibility, and plays the role of clamping the conduit.

A pair of gas pipes 6 are installed on one side of the base 1. One of the gas pipes is connected to hydrogen, which is gas, and the other is connected to oxygen, which is a combustion-supporting gas. Then one end of the gas pipe is connected with a burner 61, which is smaller and can be used smoothly. The gas pipe is connected to a gas source through a gas valve, the gas source is hydrogen, and the auxiliary gas source is oxygen. Burns to produce a flame for the disinfection of catheters and is free of soot.

Use the above-mentioned device to carry out the method for aseptic filling, as shown in Figure 7,

Step 1, first place the whole device in the aseptic area of the aseptic operation platform to ensure that it is in a sterile environment, and place the culture medium bottle in the constant temperature barrel of the first platform for use;

Step 2, in a sterile environment, pass the burner through the conduit and out, open the gas valve and ignite the burner, then burn the bottle cap on the conduit, and use a high-temperature flame (up to about 800 degrees) to extinguish bacteria, and twitch the gas pipe, so that the burner in the burning state retreats from the inside of the pipe. During this process, the disinfection of the inner wall of the pipe is completed. This process should be carried out slowly to ensure the best disinfection effect. One end of the catheter with a bottle cap is inserted into the culture medium bottle so as to avoid contamination;

Step 3, use a sterile suction pipe to inhale the catheter and keep the air valve in the trachea open during the suction process until the culture medium flows out from the catheter to complete the drainage work, withdraw the suction pipe, and close the air valve at the same time ,stand-by,

Step 4, place the culture dish to be filled on the second platform, and then start the drive motor, so that the second platform rises to the liquid outlet of the conduit and inserts it into the culture dish, and then opens the air valve. According to the principle of siphon, the culture medium Drop the liquid into the petri dish and finally close the air valve. After the completion, the second platform resets and removes the petri dish;

In step five, repeat step four to complete the filling of multiple petri dishes.

The disinfection and filling operations are all carried out in the aseptic workbench, which can ensure absolute sterility and fully meet the requirements of the experiment.

The above-mentioned embodiments are only descriptions of preferred implementations of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, those skilled in the art may make various modifications and improvements to the present invention. All should expand in the scope of protection as defined in the claims of the present invention.

Claims (4)

1. carry out the filling device of culture under aseptic condition, it is characterized in that, comprise

Provide support the base with installing space;

For placing the first support of medium bottle, described first support being fixed adjustable for height first platform, a medium bottle placed by described first platform, the bottleneck of described medium bottle is conical;

For the electric lifting platform of culture dish holding, described electric lifting platform comprises the second platform, lifting pin, leading screw, turbine and worm and drive motor, described second platform is arranged on base by lifting pin and linear bearing, and a leading screw coaxially arranged is fixed in the lower end of described lifting pin, be provided with silk braid in one turbine and be sleeved on leading screw, drive motor is directly connected with worm screw; A culture dish seat placed by described second platform, in described culture dish seat, places a culture dish;

The level height of described first platform is higher than the level height of the second platform;

One " n " shape grass tube, the Gao Dian inflow point of described conduit is provided with an outer uncovered conical bottle cap, and be frosted glass fitting surface in the surface that the conical bottleneck of described conical bottle cap and medium bottle conical flask matches, described bottle cap is provided with an admission port, and on described admission port, connection one is with the tracheae of air valve;

The top of described conduit is clamped by the clip on second support,

One is provided with the Gas Pipe of burner through conduit, and described Gas Pipe is connected to source of the gas by gas valve.

2. carry out the filling device of culture under a kind of aseptic condition according to claim 1, it is characterized in that, described source of the gas is hydrogen, and auxiliary source of the gas is oxygen.

3., based on carrying out the method that the filling device of culture carries out sterile filling under a kind of aseptic condition in claim 1 to 2 described in any one, comprising the steps, it is characterized in that,

Step one, device is placed in the aseptic area of sterile working platform, and is placed on by medium bottle in the temperature-constant bucket of the first platform stand-by;

Step 2, burner is passed conduit and passes, open gas valve and ignition combustion mouth, then flame sterilization is carried out to supravasal bottle cap, and twitch Gas Pipe, the burner of fired state is retreated from catheter interior, completes the sterilizing works to pipe inner wall in the process, the tube at one end with bottle cap also inserts in medium bottle by last closing gas valve;

Step 3, uses an aseptic suction pipe to conduit air-breathing and in the process of air-breathing, makes the air valve in tracheae be in open mode, until liquid medium flows out in conduit, cancel suction pipe and close gas check valve,

Step 4, culture dish to be filled is placed on the second platform, then drive motor is started, the liquid outlet making the second platform rise to conduit is inserted in culture dish, then air valve is opened, liquid medium is added dropwise in culture dish and finally closes gas check valve, after the second platform reset take off culture dish;

Step 5, repeats step 4, completes the filling of multiple culture dish.

4. a kind of method according to claim 3, is characterized in that, the temperature of described temperature-constant bucket is set to more than 60 DEG C.