CN104111344A - Fully automatic sample-injection mechanism of flow cytometer - Google Patents
Fully automatic sample-injection mechanism of flow cytometer Download PDFInfo
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- CN104111344A CN104111344A CN201410221782.1A CN201410221782A CN104111344A CN 104111344 A CN104111344 A CN 104111344A CN 201410221782 A CN201410221782 A CN 201410221782A CN 104111344 A CN104111344 A CN 104111344A
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Abstract
The invention discloses a fully automatic sample-injection mechanism of a flow cytometer. The fully automatic sample-injection mechanism comprises a rotation disc radially provided with several test tubes, an X-direction driving mechanism arranged on a fixing plate at one side of the rotation disc, a sampling pin and a Z-direction driving mechanism, wherein the sampling pin and the Z-direction driving mechanism are connected to the X-direction driving mechanism. The X-direction driving mechanism comprises an X-direction sliding rail arranged on the fixing plate, a sampling pin driving plate arranged on the X-direction sliding rail, and a steering engine connection rod driving mechanism for driving the sampling pin driving plate to carry out horizontal reciprocating motion along the sliding rail. The Z-direction driving mechanism is connected to a base of the rotation disc by a connection plate and drives the rotation disc to carry out vertical reciprocating motion along the Z direction. The connection plate is connected to the base by rotation telescopic shafts arranged along the rotation disc radial direction. Through the X-direction sliding rail on the fixing plate and the steering engine connection rod driving mechanism, the sampling pin arranged on the sampling pin driving plate is driven to carry out horizontal reciprocating motion along the X-direction sliding rail. The fully automatic sample-injection mechanism has a simple structure and a small occupation space and realizes stable sampling pin horizontal-motion.
Description
Technical field
the present invention relates to a kind of sample introduction mechanism, especially relate to the full-automatic sample introduction mechanism of a kind of flow cytometer.
Background technology
flow cytometer (Flow cytometry) is cell to be carried out to the device of automatic analysis and sorting.The a series of important biophysics of the cell dispersion that it can Quick Measurement, storage, demonstration float on a liquid, the characteristic parameter of biological chemistry aspect, and can the cell subsets of appointment therefrom be sorted out according to the parameter scope of preliminary election.
in prior art, flow cytometer has an important step to sample link before measuring, sample introduction mechanism for flow cytometer of the prior art, adopt drive motor to drive sampling probe to sample, not only increasing flow cytometer integral body takes up room, and adopt drive motor directly to drive, easily cause sampling probe motion unstable, sampling precision is low, simultaneously because flow cytometer is to sample from test tube, and the test tube bore of available technology adopting is generally narrower, when extracting liquid to be detected, cannot add rabbling mechanism, cause the data that detect inaccurate, for above-mentioned technical matters, prior art has also proposed a kind of stirring integrated sampling mechanism that samples, comprise a vibrating reed swinging, this vibrating reed is connected with sampling probe, by vibrating reed, drive sampling probe to swing or swing, swaying direction is single, mixing effect is bad, and hunting range is not easy to control, be that sampling probe is easily encountered inboard wall of test tube in the process swinging, serious even breaking-up test tube and sampling probe.
Summary of the invention
the present invention seeks to: the full-automatic sample introduction mechanism of flow cytometer that a kind of simple in structure, take up room little and good stability are provided.
technical scheme of the present invention is: the full-automatic sample introduction mechanism of a kind of flow cytometer, comprise the rotating disc that is radially distributed with some test tube mounting holes, be located at the X-direction driving mechanism on described rotating disc one side fixed head, and the sampling probe and the Z-direction driving mechanism that are connected with described X-direction driving mechanism, described X-direction driving mechanism comprises the X-direction slide rail of being located on described fixed head, be located at the sampling probe drive plate on described X-direction slide rail, and for driving described sampling probe drive plate to do the steering wheel bar-link drive of horizontal reciprocating movement along X-direction slide rail, described Z-direction driving mechanism connects the base of described rotating disc by web joint and drives described rotating disc to do the vertical to-and-fro movement of Z-direction simultaneously, and described web joint is connected with described base by the rotary extension axle radially arranging along described rotating disc.
preferably, described steering wheel bar-link drive comprises the steering wheel of being located on described sampling probe drive plate, and the two-stage connecting rod that one end is connected with described fixed head, the other end is connected with described steering wheel.
preferably, described Z-direction driving mechanism comprises the Z-direction guide rail being fixedly connected with described sampling probe drive plate, and described web joint is connected and drives described rotating disc to do vertical to-and-fro movement along Z-direction guide rail with the slide block on described Z-direction guide rail.
preferably, also comprise sampling probe vibrating mechanism, described sampling probe vibrating mechanism comprises the holder being connected with described web joint, be located at the vibrating motor on described holder, the eccentric wheel apparatus being connected with described vibrating motor output terminal, and the oscillating plate being connected with described eccentric wheel apparatus by web member, described sampling probe one end is through described oscillating plate.
preferably, between described rotating disc and its base, be provided with damping elastic piece, this damping elastic piece is the effect of vibration for rotating disc for slowing down vibration plate.
preferably, described sampling probe vibrating mechanism also comprises the electromagnet of being located on described holder, and the armature of being located at described electromagnet below, described armature one end is set on described web member, and the top of described web member is connected with described eccentric wheel apparatus is detachable by bearing.
preferably, be connected with for keeping oscillating plate to do the elastic component of horizontal eccentric motion on described oscillating plate, the top of described elastic component is connected with described holder.
more preferably, described elastic component is spring.
advantage of the present invention is:
1. the present invention adopts X-direction slide rail and the steering wheel bar-link drive be located on fixed head to drive the sampling probe of being located on sampling probe drive plate to do horizontal reciprocating movement along X-direction slide rail, not only simple in structure, and it is little to take up room, sampling probe tangential movement is also more stable;
2. the present invention is when sampling probe inserts test tube sampling, adopt the eccentric wheel apparatus in sampling probe vibrating mechanism to drive oscillating plate to do eccentric motion, thereby drive further sampling probe to do eccentric motion, and stir invisible spectro testing liquid, mixing effect is better compared with prior art, thereby has further improved sampling precision;
3. the present invention adopts the sampling probe of sampling, agitating function one, when saving equipment cost, reduce the shared space of equipment, and adopt eccentric wheel apparatus be convenient to control sampling probe in vitro wall scope with interior motion, effectively prevent that in motion process, sampling probe touches test tube wall, even cause sampling probe or test tube wall damaged, be applicable to the narrower test tube stirring sampling of bore;
4. the present invention adopts electromagnet and armature cooperation to make sampling probe after stirring completes, have automatic reset function, and sampling probe, after stirring completes, is got back to initial position automatically, and then sampling;
5. the present invention adopts elastic component connection fixing base and oscillating plate, effectively prevents that sampling probe from tilting, and assurance oscillating plate is done eccentric motion in the horizontal direction.
Accompanying drawing explanation
below in conjunction with drawings and Examples, the invention will be further described:
fig. 1 is the front view of the full-automatic sample introduction mechanism of flow cytometer of the present invention;
fig. 2 is the side view of the full-automatic sample introduction mechanism of flow cytometer of the present invention;
fig. 3 X-direction driving mechanism of the present invention, Z-direction driving mechanism and sampling probe vibrating mechanism schematic diagram;
fig. 4 is sampling probe vibrating mechanism stereographic map of the present invention;
fig. 5 is sampling probe vibrating mechanism vertical view of the present invention;
fig. 6 is the A-A face cut-open view of Fig. 5 of the present invention;
fig. 7 is the B-B face cut-open view of Fig. 5 of the present invention;
wherein: 1 rotating disc, 2 fixed heads, 3 sampling probes, 4aX is to slide rail, 4b sampling probe drive plate, 4c-1 steering wheel, 4c-2 two-stage connecting rod, 5 web joints, 6 bases, 7 rotary extension axles, 8aZ direction guiding rail, 8b slide block, 9a holder, 9b vibrating motor, 9c eccentric wheel apparatus, 9d web member, 9e oscillating plate, 9f electromagnet, 9g armature, 9h bearing, 9i spring.
Embodiment
embodiment: shown in 7, the full-automatic sample introduction mechanism of a kind of flow cytometer, comprise the rotating disc 1 that is radially distributed with some test tube mounting holes, be located at the X-direction driving mechanism on rotating disc 1 one side fixed heads 2, and the sampling probe 3 being connected with X-direction driving mechanism and Z-direction driving mechanism, X-direction driving mechanism comprises the X-direction slide rail 4a being located on fixed head 2, be located at the sampling probe drive plate 4b on X-direction slide rail 4a, and for driving sampling probe drive plate 4b to do the steering wheel bar-link drive of horizontal reciprocating movement along X-direction slide rail 4a, this steering wheel bar-link drive comprises the steering wheel 4c-1 being located on sampling probe drive plate 4b, and one end is connected with fixed head 2, the two-stage connecting rod 4c-2 that the other end is connected with steering wheel 4c-1, Z-direction driving mechanism connects rotating disc 1 by web joint 5 simultaneously base 6 driven rotary dish 1 are done the vertical to-and-fro movement of Z-direction, this Z-direction driving mechanism comprises the Z-direction guide rail 8a being fixedly connected with sampling probe drive plate 4b, web joint 5 is connected with slide block 8b on Z-direction guide rail 8a and driven rotary dish 1 is done vertical to-and-fro movement along Z-direction guide rail 8a, and web joint 5 is connected with base 6 by the rotary extension axle 7 radially arranging along rotating disc 1, the present invention adopts X-direction slide rail 4a and the steering wheel bar-link drive be located on fixed head 2 to drive the sampling probe 3 of being located on sampling probe drive plate 4b to do horizontal reciprocating movement along X-direction slide rail 4a, not only simple in structure, and it is little to take up room, sampling probe 3 tangential movements are also more stable.
with reference to shown in Fig. 3 to 7, the full-automatic sample introduction mechanism of flow cytometer of the present invention also comprises sampling probe vibrating mechanism, sampling probe vibrating mechanism comprises the holder 9a being connected with web joint 5, be located at the vibrating motor 9b on holder 9a, the eccentric wheel apparatus 9c being connected with vibrating motor 9b output terminal, and the oscillating plate 9e being connected with eccentric wheel apparatus 9c by web member 9d, sampling probe 3 one end are through oscillating plate 9e, when sampling probe 3 inserts test tube sampling, adopt the eccentric wheel apparatus 9c in sampling probe vibrating mechanism to drive oscillating plate 9e to do eccentric motion, thereby drive further sampling probe 3 to do eccentric motion, and stir invisible spectro testing liquid, mixing effect is better compared with prior art, thereby further improved sampling precision, adopt sampling simultaneously, the sampling probe of agitating function one, when saving equipment cost, reduce the shared space of equipment, and adopt eccentric wheel apparatus 9c be convenient to control sampling probe 3 in vitro wall scope with interior motion, effectively prevent that in motion process, sampling probe 3 touches test tube wall, even cause sampling probe 3 or test tube wall damaged, be applicable to the narrower test tube stirring sampling of bore, this sampling probe vibrating mechanism also comprises the electromagnet 9f being located on holder 9a, and the armature 9g that is located at electromagnet 9f below, armature 9g one end is set on web member 9d, the top of this web member 9d is connected with eccentric wheel apparatus 9c is detachable by bearing 9h, the present invention adopts electromagnet 9f and armature 9g cooperation to make sampling probe 3 after stirring completes, have automatic reset function, be sampling probe 3 after stirring completes, automatically get back to initial position, and then sampling, between rotating disc 1 and its base 6, be provided with damping elastic piece (not drawing on figure) simultaneously, this damping elastic piece is the effect of vibration for rotating disc 1 for slowing down vibration plate 9e, on this oscillating plate 9e, be connected with for keeping oscillating plate 9e to be the spring 9i of horizontal eccentric motion, the top of described spring 9i is connected with described holder 9a, adopt spring 9i connection fixing base 9a and oscillating plate 9e, effectively prevent that sampling probe 3 from tilting, guarantee that oscillating plate 9e does eccentric motion in the horizontal direction.
above-described embodiment is only explanation technical conceive of the present invention and feature, and its object is to allow person skilled in the art can understand content of the present invention and implement according to this, can not limit the scope of the invention with this.All equivalences that Spirit Essence is done according to the present invention change or modify, within all should being encompassed in protection scope of the present invention.
Claims (8)
1. the full-automatic sample introduction mechanism of flow cytometer, comprise the rotating disc (1) that is radially distributed with some test tube mounting holes, be located at the X-direction driving mechanism on described rotating disc (1) one side fixed head (2), and the sampling probe (3) and the Z-direction driving mechanism that are connected with described X-direction driving mechanism, it is characterized in that, described X-direction driving mechanism comprises the X-direction slide rail (4a) of being located on described fixed head (2), be located at the sampling probe drive plate (4b) on described X-direction slide rail (4a), and for driving described sampling probe drive plate (4b) to do the steering wheel bar-link drive of horizontal reciprocating movement along X-direction slide rail (4a), described Z-direction driving mechanism is connected the base (6) of described rotating disc (1) and is driven described rotating disc (5) to do the vertical to-and-fro movement of Z-direction by web joint (5) simultaneously, and described web joint (5) is connected with described base (6) by the rotary extension axle (7) radially arranging along described rotating disc (1).
2. the full-automatic sample introduction mechanism of flow cytometer according to claim 1, it is characterized in that, described steering wheel bar-link drive comprises the steering wheel (4c-1) of being located on described sampling probe drive plate (4b), and the two-stage connecting rod (4c-2) that one end is connected with described fixed head (2), the other end is connected with described steering wheel (4c-1).
3. the full-automatic sample introduction mechanism of flow cytometer according to claim 1, it is characterized in that, described Z-direction driving mechanism comprises the Z-direction guide rail (8a) being fixedly connected with described sampling probe drive plate (4b), and described web joint (5) is connected and drives described rotating disc (1) to do vertical to-and-fro movement along Z-direction guide rail (8a) with the slide block (8b) on described Z-direction guide rail (8a).
4. the full-automatic sample introduction mechanism of flow cytometer according to claim 1, it is characterized in that, also comprise sampling probe vibrating mechanism, described sampling probe vibrating mechanism comprises the holder (9a) being connected with described web joint (5), be located at the vibrating motor (9b) on described holder (9a), the eccentric wheel apparatus (9c) being connected with described vibrating motor (9b) output terminal, and the oscillating plate (9e) being connected with described eccentric wheel apparatus (9c) by web member (9d), described sampling probe (3) one end is through described oscillating plate (9e).
5. the full-automatic sample introduction mechanism of flow cytometer according to claim 4, is characterized in that, between described rotating disc (1) and its base (6), is provided with damping elastic piece.
6. the full-automatic sample introduction mechanism of flow cytometer according to claim 4, it is characterized in that, described sampling probe vibrating mechanism also comprises the electromagnet (9f) of being located on described holder (9a), and the armature (9g) of being located at described electromagnet (9f) below, it is upper that described armature (9g) one end is set in described web member (9d), and the top of described web member (9d) is by bearing (9h) and detachable connection of described eccentric wheel apparatus (9c).
7. the full-automatic sample introduction mechanism of flow cytometer according to claim 4, it is characterized in that, on described oscillating plate (9e), be connected with for keeping oscillating plate (9e) to do the elastic component of horizontal eccentric motion, the top of described elastic component is connected with described holder (9a).
8. the full-automatic sample introduction mechanism of flow cytometer according to claim 7, is characterized in that, described elastic component is spring (9i).
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| CN201410221782.1A CN104111344B (en) | 2014-05-23 | 2014-05-23 | The full-automatic sample introduction mechanism of a kind of flow cytometer |
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| CN201410221782.1A CN104111344B (en) | 2014-05-23 | 2014-05-23 | The full-automatic sample introduction mechanism of a kind of flow cytometer |
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| CN104111344A true CN104111344A (en) | 2014-10-22 |
| CN104111344B CN104111344B (en) | 2016-04-13 |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104913946A (en) * | 2015-05-26 | 2015-09-16 | 中山市创艺生化工程有限公司 | A grasping device for blood cell analyzer |
| CN106706370A (en) * | 2016-12-30 | 2017-05-24 | 聚光科技(杭州)股份有限公司 | Automatic water quality sampler and operating method thereof |
| CN108303556A (en) * | 2018-01-10 | 2018-07-20 | 中南林业科技大学 | A kind of medicinal intelligent biochemical detection system |
| CN109781609A (en) * | 2018-12-31 | 2019-05-21 | 苏州朗如精密机械科技有限公司 | A kind of hit impact protection formula sampler of stream type cell analyzer |
| CN109883895A (en) * | 2019-02-11 | 2019-06-14 | 辽宁科技大学 | A kind of mixed particle layer test device and method |
| CN110079446A (en) * | 2019-02-28 | 2019-08-02 | 保定蕴生生物科技有限公司 | Microbial fermentation product monitors fermentor on-line |
| CN111089981A (en) * | 2019-12-25 | 2020-05-01 | 中生（苏州）医疗科技有限公司 | Automatic sample loading device of carousel |
| CN111521547A (en) * | 2020-05-13 | 2020-08-11 | 洹仪科技(上海)有限公司 | Particle analyzing and sorting device and method |
| CN111796109A (en) * | 2020-07-13 | 2020-10-20 | 杭州道宇检测技术有限公司 | Automatic sample introduction device of atomic absorption spectrophotometer |
| CN112834413A (en) * | 2021-03-04 | 2021-05-25 | 常州必达科生物科技有限公司 | Fully automatic flow cytometer |
| CN112945836A (en) * | 2021-02-03 | 2021-06-11 | 上海萌薇生物医疗科技有限公司 | Automatic device of taking in and send out of buckle centre gripping formula cell counting board |
| CN114252298A (en) * | 2021-12-21 | 2022-03-29 | 宁波甬恒瑶瑶智能科技有限公司 | Automatic flow cytometer pretreatment equipment |
| CN114350486A (en) * | 2020-10-13 | 2022-04-15 | 中国科学院大连化学物理研究所 | A spray needle interface arrangement for unicellular sample |
| CN117169535A (en) * | 2023-09-01 | 2023-12-05 | 俊兰(丽水)医院有限公司 | Sample adding device for medical examination |
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Cited By (19)
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| CN104913946B (en) * | 2015-05-26 | 2017-07-11 | 中山市创艺生化工程有限公司 | A grabbing device for hematology analyzer |
| CN104913946A (en) * | 2015-05-26 | 2015-09-16 | 中山市创艺生化工程有限公司 | A grasping device for blood cell analyzer |
| CN106706370A (en) * | 2016-12-30 | 2017-05-24 | 聚光科技(杭州)股份有限公司 | Automatic water quality sampler and operating method thereof |
| CN108303556A (en) * | 2018-01-10 | 2018-07-20 | 中南林业科技大学 | A kind of medicinal intelligent biochemical detection system |
| CN109781609A (en) * | 2018-12-31 | 2019-05-21 | 苏州朗如精密机械科技有限公司 | A kind of hit impact protection formula sampler of stream type cell analyzer |
| CN109883895A (en) * | 2019-02-11 | 2019-06-14 | 辽宁科技大学 | A kind of mixed particle layer test device and method |
| CN109883895B (en) * | 2019-02-11 | 2023-09-05 | 辽宁科技大学 | Mixed particle layering test device and method |
| CN110079446B (en) * | 2019-02-28 | 2022-05-17 | 保定蕴生生物科技有限公司 | Fermentation tank for on-line monitoring of microbial fermentation products |
| CN110079446A (en) * | 2019-02-28 | 2019-08-02 | 保定蕴生生物科技有限公司 | Microbial fermentation product monitors fermentor on-line |
| CN111089981A (en) * | 2019-12-25 | 2020-05-01 | 中生（苏州）医疗科技有限公司 | Automatic sample loading device of carousel |
| CN111521547A (en) * | 2020-05-13 | 2020-08-11 | 洹仪科技(上海)有限公司 | Particle analyzing and sorting device and method |
| CN111796109A (en) * | 2020-07-13 | 2020-10-20 | 杭州道宇检测技术有限公司 | Automatic sample introduction device of atomic absorption spectrophotometer |
| CN111796109B (en) * | 2020-07-13 | 2023-10-10 | 杭州道宇检测技术有限公司 | Automatic sample injection device of atomic absorption spectrophotometer |
| CN114350486A (en) * | 2020-10-13 | 2022-04-15 | 中国科学院大连化学物理研究所 | A spray needle interface arrangement for unicellular sample |
| CN112945836A (en) * | 2021-02-03 | 2021-06-11 | 上海萌薇生物医疗科技有限公司 | Automatic device of taking in and send out of buckle centre gripping formula cell counting board |
| CN112834413A (en) * | 2021-03-04 | 2021-05-25 | 常州必达科生物科技有限公司 | Fully automatic flow cytometer |
| CN114252298A (en) * | 2021-12-21 | 2022-03-29 | 宁波甬恒瑶瑶智能科技有限公司 | Automatic flow cytometer pretreatment equipment |
| CN117169535A (en) * | 2023-09-01 | 2023-12-05 | 俊兰(丽水)医院有限公司 | Sample adding device for medical examination |
| CN117169535B (en) * | 2023-09-01 | 2024-02-27 | 俊兰(丽水)医院有限公司 | Sample adding device for medical examination |
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