CN104094939B - A kind of containing copper 8-hydroxyquinolinate with the Recompounded pesticide of Difenoconazole - Google Patents

Abstract

The invention relates to a compound pesticide containing copper quinolate and difenoconazole, which belongs to the technical field of compound pesticides. It contains copper quinolate and difenoconazole, and the weight ratio of copper quinolate and difenoconazole is 1:10˜10:1. The above-mentioned compound pesticide containing copper quinolate and difenoconazole, through the rational compounding of copper quinolate and difenoconazole, copper quinolate and difenoconazole can produce complementary effects, so that quinoline Copper effectively makes up for the deficiency of difenoconazole in the control of low fungi such as Phytophthora and Pythium, and has a significant synergistic effect, expanding the scope of control and improving the efficacy.

Description

A compound pesticide containing copper quinolate and difenoconazole

technical field

The invention belongs to the technical field of compound pesticides, in particular to a compound pesticide containing copper quinolate and difenoconazole.

Background technique

Copper quinolate (copper hydroxyquinolate; copper 8-quinolinate), the chemical name is bis(8-quinolinyl)copper, and its structural formula is:

.

It is an organic chelated copper fungicide, which is commonly used by manufacturers as a seed treatment agent to prevent and control various seed-borne diseases. It is effective against Phytophthora, Pythium, Alternaria, Botrytis, and Phoma It has a certain effect and is often used in combination with other insecticides and fungicides.

Difenoconazole (Difenoconazole; Difenoconazole), chemical name is cis, trans-3-chloro-4-[4-methyl-2-1H-1,2,4-triazol-1-yl Methyl)-1,3-dipentan-2-yl)phenyl 4-chlorophenyl ether, the structural formula is:

.

It is a high-efficiency broad-spectrum triazole fungicide with both protective and therapeutic effects. It has long-lasting protective and therapeutic effects on ascomycetes, basidiomycetes, various semi-known fungi and various seed-borne pathogenic fungi. It has good systemic property and is safe for crops, and is suitable for use as a seed treatment agent.

The single use of copper quinolate and difenoconazole has the disadvantages of insufficient control effect on low-grade fungi such as Phytophthora and Pythium, small control range, and low drug efficacy.

Contents of the invention

Aiming at the problems existing in the prior art, the object of the present invention is to design and provide a technical solution for compound pesticides containing copper quinolate and difenoconazole.

A described compound pesticide containing copper quinolate and difenoconazole is characterized in that it contains copper quinolate and difenoconazole, and the weight ratio of copper quinolate and difenoconazole is 1:10～10:1.

The compound pesticide containing copper quinolate and difenoconazole is characterized in that the weight ratio of copper quinolate and difenoconazole is 1:9-9:1.

The compound pesticide containing copper quinolate and difenoconazole is characterized in that the weight ratio of copper quinolate and difenoconazole is 1:4-4:1.

The compound pesticide containing copper quinolate and difenoconazole is characterized in that the weight ratio of copper quinolate and difenoconazole is 3:7-7:3.

The compound pesticide containing copper quinolate and difenoconazole is characterized in that the weight ratio of copper quinolate and difenoconazole is 2:3-3:2.

The above-mentioned compound pesticide containing copper quinolate and difenoconazole, through the rational compounding of copper quinolate and difenoconazole, copper quinolate and difenoconazole can produce complementary effects, so that quinoline Copper effectively makes up for the deficiency of difenoconazole in the control of low fungi such as Phytophthora and Pythium, and has a significant synergistic effect, expanding the scope of control and improving the efficacy.

detailed description

The present invention will be further described below in conjunction with the examples.

Example 1 Copper quinolate and difenoconazole compound pesticide, wherein the ratio of copper quinolate and difenoconazole is 1:10-10:1.

Example 2 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 45%, and the active ingredient content of difenoconazole is 5%.

Example 3 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 40%, and the active ingredient content of difenoconazole is 10%.

Example 4 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 35%, and the active ingredient content of difenoconazole is 15%.

Example 5 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 30%, and the active ingredient content of difenoconazole is 20%.

Example 6 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 25%, and the active ingredient content of difenoconazole is 25%.

Example 7 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 20%, and the active ingredient content of difenoconazole is 30%.

Example 8 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 15%, and the active ingredient content of difenoconazole is 35%.

Example 9 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 10%, and the active ingredient content of difenoconazole is 40%.

Example 10 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 5%, and the active ingredient content of difenoconazole is 45%.

Example 11 The total active ingredient content of the medicament is 50%, the active ingredient content of quinoline copper is 45%, and the active ingredient content of difenoconazole manganese salt is 5%.

Example 12 The total active ingredient content of the medicament is 50%, the active ingredient content of quinoline copper is 40%, and the active ingredient content of difenoconazole manganese salt is 10%.

Example 13 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 35%, and the active ingredient content of difenoconazole manganese salt is 15%.

Example 14 The total active ingredient content of the medicament is 50%, the active ingredient content of quinoline copper is 30%, and the active ingredient content of difenoconazole manganese salt is 20%.

Example 15 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 25%, and the active ingredient content of difenoconazole manganese salt is 25%.

Example 16 The total active ingredient content of the medicament is 50%, the active ingredient content of quinoline copper is 20%, and the active ingredient content of difenoconazole manganese salt is 30%.

Example 17 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 15%, and the active ingredient content of difenoconazole manganese salt is 35%.

Example 18 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 10%, and the active ingredient content of difenoconazole manganese salt is 40%.

Example 19 The total active ingredient content of the medicament is 50%, the active ingredient content of copper quinolate is 5%, and the active ingredient content of difenoconazole manganese salt is 45%.

Example 20 The total active ingredient content of the medicament is 20%, the active ingredient content of copper quinolate is 5%, and the active ingredient content of difenoconazole manganese salt is 15%.

Example 21 The total active ingredient content of the medicament is 20%, the active ingredient content of copper quinolate is 8%, and the active ingredient content of difenoconazole manganese salt is 12%.

Example 22 The total active ingredient content of the medicament is 20%, the active ingredient content of quinoline copper is 10%, and the active ingredient content of difenoconazole manganese salt is 10%.

Example 22 The total active ingredient content of the medicament is 20%, the active ingredient content of copper quinolate is 12%, and the active ingredient content of difenoconazole manganese salt is 8%.

Example 22 The total active ingredient content of the medicament is 20%, the active ingredient content of copper quinolate is 15%, and the active ingredient content of difenoconazole manganese salt is 5%.

Example 23 The total active ingredient content of the medicament can be prepared at any concentration between 5% and 90%, and the ratio between the two is similar or the same as that of Examples 1 to 22, but not limited to the above concentrations.

Example 24 Control effect of quinoline copper and difenoconazole compound seed dressing on rice seedling blight

1. Experimental species

Longjing 40

2. Treatment settings and test methods

Treatment 1 50% quinoline (copper quinolate 15%, difenoconazole 35%) 1:200 seed dressing

Treatment 2 50% quinoline benzene (20% copper quinoline, 30% difenoconazole) 1:200 seed dressing

Treatment 3 50% quinoline (copper quinolate 25%, difenoconazole 25%) 1:200 seed dressing

Treatment 4 50% quinoline (copper quinolate 30%, difenoconazole 20%) 1:200 seed dressing

Treatment 5 50% quinoline (copper quinolate 40%, difenoconazole 10%) 1:200 seed dressing

Treatment 6 75% quinoline (copper quinolate 25%, difenoconazole 50%) 1:300 seed dressing

Treatment 7 75% quinoline (copper quinolate 30%, difenoconazole 45%) 1:300 seed dressing

Treatment 8 75% quinoline (copper quinolate 40%, difenoconazole 35%) 1:300 seed dressing

Treatment 9 75% quinoline (copper quinolate 50%, difenoconazole 25%) 1:300 seed dressing

Control 1 50% quinoline copper wettable powder 1:200 seed dressing

Control 2 10% difenoconazole water dispersible granule 1:40 seed dressing

Control 3 clear water

Soak the rice seeds for 48 hours and then pick them up, add medicine or water for control, add appropriate amount of water, stir evenly, dry and sow. Before rice transplanting, the incidence of rice blight was investigated, and the growth status, uniformity and root development of the seedlings were observed at the same time.

And calculate the control effect of rice disease according to the following formula:

3. Test results

The control effects of each pesticide treatment and control on rice blight are shown in Table 1:

Table 1 Control effect of compound seed dressing of copper quinolate and difenoconazole on rice seedling blight

From the test results, in the case of the same active ingredient content, the control effect of seed dressing on rice damping-off by using various treatments is between 89.56% and 97.18%, which is much higher than 74.28% of the control agent 50% quinoline copper suspension concentrate. % and 10% difenoconazole water dispersible granules were 82.67, indicating that the combination of the two had a strong synergistic effect on the control effect of rice blight.

The seedlings of each treatment were neat, the leaf color was dark green, and the root system was well developed, which was not significantly different from the control.

Example 25 Control effect of copper quinolate and difenoconazole compound soaking seeds on rice bakanae disease

1. Test varieties

Kim Zao 47

2. Treatment settings and test methods

Treatment 1 40% quinoline (copper quinolate 35%, difenoconazole 5%) 4000 times solution

Treatment 2 40% quinoline (copper quinolate 30%, difenoconazole 10%) 4000 times solution

Treatment 3 40% quinoline (copper quinolate 25%, difenoconazole 15%) 4000 times solution

Treatment 4 40% quinoline (copper quinolate 20%, difenoconazole 20%) 4000 times solution

Treatment 5 40% quinoline (copper quinolate 15%, difenoconazole 25%) 4000 times solution

Treatment 6 40% quinoline (copper quinolate 10%, difenoconazole 30%) 4000 times solution

Treatment 7 40% quinoline (copper quinolate 5%, difenoconazole 35%) 4000 times solution

Treatment 8 60% quinoline (copper quinolate 35%, difenoconazole 25%) 6000 times liquid

Treatment 9 60% quinoline (copper quinolate 25%, difenoconazole 35%) 6000 times liquid

Control 1 50% quinoline copper wettable powder 5000 times liquid

Control 2 10% difenoconazole water dispersible granules 1000 times liquid

Control 3 clear water

The rice seeds were soaked in medicine for 48 hours, and then germinated until the buds were 0.5 cm long, and then sowed on the seedling tray after being slightly dried. Each handles 3 seedling trays, 80g seeds per tray, and adopts the seedling raising method of dry seedling raising.

3. Survey

When the length of diseased seedlings is significantly higher than that of normal seedlings, investigate the number of diseased plants. Calculate the incidence rate.

Calculate the effectiveness according to the following formula:

4. Test results:

Each chemical treatment showed excellent control effects, as shown in Table 2.

Table 2 Control effect of copper quinolate and difenoconazole compound on rice bakanae disease

Pharmacy treatment Active ingredient concentration (PPM) Prevention effect (%) 1 100 91.46 2 100 95.11 3 100 90.93 4 100 94.42 5 100 89.78 6 100 87.60 7 100 86.74 8 100 91.51 9 100 88.60 CK1 100 72.25 CK2 100 81.41

It can be seen from the test results that each compound treatment has shown excellent control effect, the control effect is between 86.74% and 95.11%, which is far greater than the 72.25% of the single agent of quinoline copper and diphenoxymethyl with the same active ingredient concentration. 81.41% of cycloconazole single agent, indicating that the combination of copper quinolate and difenoconazole showed a strong synergistic effect on the control effect of rice bakanae disease.

Example 26 Control effect of copper quinolate and difenoconazole compound on rice false smut and ear rot

1. Test varieties

"Yongyou 12", a hybrid late japonica variety susceptible to rice false smut

2. Treatment settings and test methods

Treatment 1 50% quinoline (copper quinolate 35%, difenoconazole 15%) 300 g/ha

Treatment 2 50% quinoline (copper quinolate 30%, difenoconazole 20%) 300 g/ha

Treatment 3 50% quinoline (copper quinolate 25%, difenoconazole 25%) 300 g/ha

Treatment 4 50% quinoline (copper quinolate 15%, difenoconazole 35%) 300 g/ha

Treatment 5 50% quinoline (copper quinolate 10%, difenoconazole 40%) 300 g/ha

Treatment 6 50% quinoline (copper quinolate 5%, difenoconazole 45%) 300 g/ha

Control 1 25% quinoline copper suspension concentrate 600 g/ha

Control 2 10% difenoconazole water dispersible granule 1500 g/ha

Each treatment was repeated 3 times, and a total of 24 plots were set up, each plot was 30 square meters.

3. How to use the medicine

Water 45 kg/mu, and spray one week before the rice breaks.

4. Survey and survey method

35 days after the application, 5 points were selected in each plot, and 200 ears were surveyed at each point, and the incidence of rice false smut and rice ear rot was investigated according to the corresponding grading standards.

The grading standard of rice false smut is shown in the table below

disease level 0 1 3 5 7 9 Number of diseased grains/ear 0 1 2-4 5-8 9-13 ≥14 Ear Weight Loss % 0 0.1-5.0 5.1-10.0 10.1-20.0 20.1-50.0 ≥50.1

Grading standards for rice ear rot are shown in the table below:

disease level Grading standards level 0 no lesions Level 1 Less than 1% loss per ear Level 3 Loss of 2% to 5% per ear Level 5 Loss of 6% to 15% per ear Level 7 Loss of 16% to 25% per ear Level 9 More than 26% loss per ear

Calculation formula and statistical analysis:

5. Test results

The control results of copper quinolate and difenoconazole compound and each positive control agent on rice false smut and rice ear rot are shown in Table 3:

Table 3 Control effect of copper quinolate and difenoconazole compound on rice false smut and ear rot

Pharmacy treatment Active ingredient dosage (g/ha) Control effect on rice false smut Control effect on rice ear rot 1 150 89.68 88.51 2 150 86.31 90.31 3 150 86.73 86.54 4 150 86.66 90.03 5 150 88.25 86.61 6 150 89.45 87.35 CK1 150 76.34 74.92 CK2 150 71.21 66.53

In terms of the control effect on rice false smut, the control effects of copper quinolate and difenoconazole are between 86.31% and 89.68%, which are much higher than the 76.34% and 71.21% of the two single agent treatments, indicating that Copper quinolate and difenoconazole showed a strong synergistic effect on the control effect of rice false smut. In the control effect of rice ear rot, the control effect of copper quinolate and difenoconazole is between 86.54% and 90.31%, which is much higher than the 66.53% to 74.92% of the two single agent control treatments, indicating that copper quinolate and difenoconazole showed a synergistic effect on the control effect of rice ear rot.

This shows that the combination of copper quinolate and difenoconazole has a significant control effect on late-stage rice diseases such as rice false smut and ear rot, and the synergistic effect is obvious.

Other formulas also showed the same synergistic effect on the prevention and treatment of fungal diseases such as rice false smut and rice ear rot in the later stage of rice growth, so I won't repeat them here.

Example 27 Control effect of copper quinolate and difenoconazole compound on rice panicle blast

1. Test varieties

Super Rice Variety "Zhongzheyou No. 1"

2. Test processing settings

Treatment 1 20% quinoline (copper quinolate 15%, difenoconazole 5%) 600 g/ha

Treatment 2 20% quinoline (copper quinolate 13%, difenoconazole 7%) 600 g/ha

Treatment 3 20% quinoline (copper quinolate 10%, difenoconazole 10%) 600 g/ha

Treatment 4 20% quinoline (copper quinolate 7%, difenoconazole 13%) 600 g/ha

Treatment 5 20% quinoline (copper quinolate 5%, difenoconazole 15%) 600 g/ha

Control 1 20% quinoline copper suspension concentrate 600 g/ha

Control 2 10% difenoconazole water dispersible granule 1200 g/ha

3. How to use the medicine

Add 30 kg/mu of water, and spray at the rice breaching stage.

4. Investigation method and investigation

25 days after spraying, investigate the incidence index of rice panicle blast, and 35 days after spraying, investigate the incidence of bacterial rice blight clusters, and harvest and count yields by plot.

Grading standards for rice panicle blast are shown in the table below

disease level Injury situation of panicle blast Single ear damage 0 Disease ear rate is less than 1% Hogami disease-free 1 Disease ear rate 1-5% Loss rate per ear ≤ 5% (individual twigs are diseased) 3 Disease ear rate is 5.1-10% The loss rate per ear is 5.1-15% (about 1/10~1/5 of the branches are infected) 5 Disease ear rate is 10.1-25% The loss rate per ear is 15.1-30% (about 1/5~1/3 of the branches are diseased) 7 Disease ear rate is 25.1-50% The loss rate per ear is 30.1-50% (the ear neck or main shaft is diseased, the grain is half-shrunk) 9 Disease ear rate ≥ 50.1% Loss rate per ear ≥ 50.1% (ear neck disease, most shriveled grains or white ears)

Calculation method of control effect on rice panicle blast:

5. Test results

The control effects of quinoline copper and difenoconazole compound treatments and positive controls on rice panicle blast are shown in Table 4:

Table 4 Control effect of copper quinolate and difenoconazole compound on rice panicle blast

pesticide treatment Active ingredient dosage (g/ha) Control effect (%) 1 120 86.37 2 120 88.60 3 120 85.53 4 120 88.20 5 120 85.55 CK1 120 63.41 CK2 120 75.63

From the test results, the control effect of each treatment on rice panicle blast is between 85.53% and 88.60%, which is higher than the 63.41% and 75.63% of the two single-dose controls, indicating that the combination of copper quinolate and difenoconazole is effective for rice. The control effect of panicle blast is significant, and the synergistic effect of the combination of the two is obvious.

Example 28 The combination of quinoline copper and difenoconazole has a good control effect on rice sheath blight, rice blast, wheat ear rot, wheat sheath blight and other diseases, and the synergistic effect is obvious. I won't go into details one by one.

Example 29 Indoor bioassay of copper quinolate and difenoconazole complex on rice bakanae pathogen

1. Drug preparation

The original copper quinoline drug is made into a suspension by ultrasonic treatment, dissolved in a small amount of acetone, and diluted with sterile water to form mother solutions of 800mg/ml and 400mg/ml respectively. According to the purpose of mixing, set the ratio of multiple groups of medicines, 1:9, 1:4, 3:7, 2:3, 1:1, 3:2, 7:3, 4:1, 9:1, each ratio Both are the mass ratios of the active ingredients of the two medicaments.

2. Test strains

Rice bakanae pathogen.

3. Test method

The indoor virulence test of rice bakanae pathogen was carried out by mycelium rate method.

Under aseptic operation conditions, quantitatively add pre-thawed sterilized medium (generally 60ml) into a sterile conical flask according to the test treatment, quantitatively draw the liquid medicine from low concentration to high concentration, and add them to the above conical flasks respectively medium, shake well. Then pour it into three or more petri dishes with a diameter of 9 cm to prepare drug-containing plates of corresponding concentrations. In the experiment, the treatment without drug was used as the blank control, and each treatment had no less than 3 repetitions.

The bakanae bacteria cultured in the culture medium (about 5 days old), under aseptic conditions, use a sterilized puncher with a diameter of 5mm to cut the bacteria cake from the edge of the colony, and use an inoculator to connect the bacteria cake to the drug-containing plate In the center, the mycelium faces up, cover the dish, and place it in an incubator with a suitable temperature for cultivation.

According to the growth of bacteria in the blank control petri dish, the growth of pathogenic fungus hyphae was investigated. The colony diameter was measured with a caliper in millimeters (mm). The diameter of each colony was measured vertically once by the cross method.

According to the survey results, use formula 1 and formula 2 to calculate the mycelial growth inhibition rate of the target bacteria tested by each treatment concentration, the unit is percentage (%), and the calculation results retain two decimal places.

……………………………………………Formula 1)

In the formula:

- colony growth diameter;

- colony diameter;

- Cake diameter.

…………………………………… Formula (2)

In the formula:

- mycelial growth inhibition rate;

-Blank control colony growth diameter;

- Colony growth diameter of agent-treated colonies.

Using Tang Qiyi DPS data analysis software, according to the logarithm value of the concentration of each agent and the corresponding mycelium growth inhibition probability value for regression analysis, calculate the cost of each agent.

4. Compounding effect evaluation

Based on the EC50 value of each single agent and compound agent, the synergistic coefficient (SR) of each compound agent was calculated according to Wadley method, and the compound effect was evaluated by SR value. SR≥1.5 means synergistic effect; SR≤0.5 means antagonistic effect; 0.5<SR<1.5 means additive effect.

The synergistic factor (SR) is calculated according to formula (6) and formula (7):

………………………………… Formula (3)

In the formula:

- The theoretical value of the mixture in milligrams per liter (mg/L);

- The percentage content of A in the mixture, in percentage (%);

- The percentage of B in the mixture, in percentage (%);

- The theoretical value of A in the mixture in milligrams per liter (mg/L);

- The theoretical value of B in the mixture in milligrams per liter (mg/L).

………………………………………………… Formula (4)

In the formula:

- the synergistic coefficient of the mixture;

-Theoretical value of the mixture, in milligrams per liter (mg/L);

- The measured value of the mixture, in milligrams per liter (mg/L).

5. Test results

The indoor bioassay results of quinolate copper and difenoconazole complex against rice bakanae pathogen are shown in the table below:

Test drug Actual _EC50 (ppm) Theoretical _EC50 (ppm) Synergy factor copper quinolate 5.35 / / Difenoconazole 0.096 / / Quinobenol ratio 1:9 0.034 0.106 3.09 Quinabenol ratio 1:4 0.037 0.119 3.24 Quinabenol ratio 3:7 0.030 0.136 4.50 Quinabenol ratio 2:3 0.029 0.158 5.40 Quinabenol ratio 1:1 0.027 0.189 7.11 Quinabenol ratio 3:2 0.038 0.234 6.07 Quinabenol ratio 7:3 0.050 0.307 6.11 Quinabenol ratio 4:1 0.084 0.448 5.34 Quinabenol ratio 9:1 0.133 0.826 6.23

According to the test results, the inhibitory efficiency of difenoconazole against rice bakanae is greater than that of copper quinolate, but the synergistic coefficient of the combination of the two is between 3.09 and 7.11, far greater than 1.5, indicating that difenoconazole Cycloconazole and quinoline copper compound showed a strong synergistic effect.

Example 30 Indoor bioassay of difenoconazole and copper quinolate against rice blast fungus

1. Drug preparation

Same as Example 29

2. Test strains

Rice blast pathogen strain: Pyriculariaoryae WZ30, which belongs to the strong pathogenic strain of ZB population.

3. Test method

The cultured rice blast pathogenic fungal spores were eluted from the culture medium or diseased tissue with deionized water, filtered, centrifuged (1000r/min) for 5min, poured off the clear night, added deionized water, and centrifuged again. Finally, resuspend the spores with deionized water to 1×10 ⁵ -1×10 ⁷ spores per ml, and add 0.5% glucose solution.

Use a pipette or pipette from low concentration to high concentration, sequentially draw 0.5mL of the drug solution into small test tubes, and then draw 0.5mL of the prepared spore suspension, so that the drug solution and the spore suspension are mixed evenly in equal amounts. Use a micro-sampler to draw the above-mentioned mixed medicinal solution onto the concave glass slide, then place it in a fresh-keeping box with a shallow layer of water, cover it to keep it moist and culture it in an incubator at a suitable temperature. Each treatment was repeated no less than 3 times, and the treatment without drug was used as the blank control.

When the blank control spore germination rate reaches more than 90%, check the spore germination of each treatment

When each treatment repeats random observation of more than 3 fields of view, the total number of spores investigated is not less than 200, and the number of germination and the total number of spores are recorded respectively. The length of the spore germ tube is greater than the short radius of the spore, which is regarded as germination.

According to the survey data, calculate the relative inhibition rate of spore germination of each treatment, the unit is percentage (%). Calculated according to formulas (5), (6) and (7), the calculation results retain two decimal places:

……………………………………… Formula (5)

In the formula:

- spore germination rate;

- Number of spores germinated

- Total number of spores investigated

……………………………………… Formula (6)

In the formula

- Treatment corrected spore germination rate

- Handle spore germination rate

- Blank control spore germination rate

……………………………………Formula (7)

In the formula

- relative inhibition rate of spore germination;

- blank control spore germination rate;

- Process corrected spore germination rate.

According to the logarithmic value of each agent concentration and the probability value of the corresponding relative inhibition rate of spore germination, regression analysis is performed to calculate the value of each agent.

4. Compounding effect evaluation

Same as Example 29.

5. Test results

The indoor toxicity test results of difenoconazole and quinoline copper compound to rice blast fungus are shown in the following table:

Test drug Actual _EC50 (ppm) Theoretical _EC50 (ppm) Synergy factor copper quinolate 10.07 / / Difenoconazole 0.125 / / Quinobenol ratio 1:9 0.089 0.139 1.55 Quinabenol ratio 1:4 0.045 0.156 3.49 Quinabenol ratio 3:7 0.069 0.178 2.57 Quinabenol ratio 2:3 0.049 0.207 4.2 Quinabenol ratio 1:1 0.067 0.247 3.69 Quinabenol ratio 3:2 0.242 0.307 1.27 Quinabenol ratio 7:3 0.182 0.405 2.23 Quinabenol ratio 4:1 0.213 0.595 2.79 Quinabenol ratio 9:1 0.450 1.124 2.5

According to the test results, the inhibitory efficiency of difenoconazole against rice blast fungus is greater than that of copper quinolate, but the synergistic coefficient of the two compounds is 1.27 except that the ratio of quinolate to benzene is 1.27, which shows an additive effect. In addition, the other formulas were all greater than 1.5, indicating that the combination of difenoconazole and copper quinolate showed a strong synergistic effect on the inhibition of bakanae pathogen.

Example 31 Indoor bioassay of difenoconazole and copper quinolate compound on rice smut fungus

1. Drug preparation

Same as Example 29

2. Test strains

Oryza smut strain Ustilaginoideavirens UV-13

3. Test method

Same as Example 29

4. Compounding effect evaluation

Simultaneous instance 29

5. Test results

The indoor bioassay results of difenoconazole and copper quinolate on rice smut fungus are shown in the table below:

According to the test results, the EC ₅₀ of difenoconazole and copper quinolate against rice false smut were 0.031 and 3.91, showing strong inhibitory effects. The compound synergistic coefficient of the two is between 3.71 and 6.58, and the synergistic effect is obvious.

Claims (2)

1. a compound pesticide containing copper quinoline and difenoconazole is characterized in that containing copper quinoline and difenoconazole, and the weight ratio of copper quinoline and difenoconazole is 1 :9～2:3. 2. A compound pesticide containing copper quinoline and difenoconazole is characterized in that it contains copper quinoline and difenoconazole, and the weight ratio of copper quinoline and difenoconazole is 3 :2～9:1.