CN104089804B - Biological sample processing system - Google Patents
Biological sample processing system Download PDFInfo
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- CN104089804B CN104089804B CN201410311189.6A CN201410311189A CN104089804B CN 104089804 B CN104089804 B CN 104089804B CN 201410311189 A CN201410311189 A CN 201410311189A CN 104089804 B CN104089804 B CN 104089804B
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of biological sample processing systems, including system backplane, glass slide placement platform, sample cell, biological sample drips sheet devices, sample treatment cavity, control system and man-machine interface, it includes spatial movement mechanism that biological sample, which drips sheet devices, liquid-transfering device and glass slide fetching device, control system passes through control spatial movement mechanism, the motion parts of spatial movement mechanism are made to export the motion profile of setting, when testing beginning, it is automatic to place glass slide, and makes liquid-transfering device for biological sample automatic sucking and be automatically transferred on the surface of glass slide, after the completion of experiment, it is automatic to remove glass slide；The temperature of biological sample tank is adjustable.Biological sample processing system of the present invention can carry out the operation of chromosome automatic drip piece with high throughput, it is small in size, production cost is low, installation and easy to maintain, low energy consumption, especially suitable for CYTOGENETIC ANALYSIS OF ONE, it realizes that chromosome drips piece process automation, improves analysis efficiency, ensure that biological sample handles quality.
Description
Technical field
The present invention relates to a kind of biological sample experimental provisions, the automatic drip piece operation suitable for biological sample preparation process
Technical field and cytogenetics experimental technique field.
Background technique
According to classical cytogenetics, the chromosome prepared from cell can be analyzed under the microscope, to possible
Existing defect is diagnosed.Chromosome preparation need to carry out the cell of harvest hypotonic, fixed, dry, aging, enzymic digestion and
The processing such as aobvious band, after these processing, just can under the microscope analyze the chromosome of cell, to judge whether to deposit
In chromosome abnormality.This classical method is continued to use always till now at the beginning of foundation.
In the past few decades, Cytogenetic techniques achieve various important breakthroughs, as cell synchronization is trained
The technology of supporting, chromosome high resolution technology and amniocyte chromosome analysis technology in situ etc..Although in Cytogenetic techniques side
Face has achieved these great breakthroughs, but to reach optimal Chromosome spread degree and its high consistency and repeatability
It is still extremely difficult problem, it is difficult to ensure that each as the consistency and again of its Chromosome spread of the sample of cytogenetic
Renaturation.
According to Chromosome spread principle of dynamics, in conventional dyeing production procedure, the temperature and humidity pair of environment
There is very big influence in the quality of chromosome preparation.To solve influence of the environmental factor to Chromosome spread degree, the happy occasion biology in Shanghai
The Bioexperiment instrument of the entitled Chromprep S of product of Science and Technology Ltd.'s manufacture, the manufacture of Euroclone company, Italy
The entitled Optichrome Bioexperiment instrument of product, Thermotron Cytogenetic chamber company, the U.S. and Japan
Hanabin Metaphase spreader company develops manually Chromosome spread instrument respectively, very good solution environment
Influence of the factor to Chromosome spread, it is ensured that the preparation quality of chromosome.
With increasing for analytical specimen amount, manual Chromosome spread instrument is increasingly unable to satisfy the need of routine analysis work
It asks.Japanese Hanabin company develops automatic staining body drop piece instrument Metaphase Auto-spreader, solves dye substantially
The high-throughput problem of colour solid preparation, but the system is a site type machine, and volume is larger, and structure is complicated, and energy consumption is higher, raw
Production, operation and maintenance cost are very high.
Summary of the invention
In order to solve prior art problem, it is an object of the present invention to overcome the deficiencies of the prior art, and to provide one kind
Biological sample processing system can carry out the operation of chromosome automatic drip piece with high throughput, small in size, production cost is low, installation and
Easy to maintain, low energy consumption.The present invention is especially suitable for CYTOGENETIC ANALYSIS OF ONEs, realize the automation of chromosome drop piece process, mention
High analyte efficiency ensure that the quality of biological sample processing.
Purpose is created to reach foregoing invention, the present invention adopts the following technical solutions:
A kind of biological sample processing system, including system backplane, glass slide placement platform, sample cell, biological sample drip piece
Device and sample treatment chamber, glass slide placement platform are fixedly installed in system backplane, are arranged on glass slide placement platform and are carried
Slide further includes control system and man-machine interface, and control system drips sheet devices by control biological sample, by biology to be processed
Sample is transferred in slide surface, and user realizes information exchange by man-machine interface and control system, and biological sample drips piece
Device includes spatial movement mechanism, liquid-transfering device and glass slide fetching device, and the fixed part of spatial movement mechanism passes through bracket
It is fixedly connected with system backplane, the bottom of bracket is fixedly installed in system backplane, the action part difference of spatial movement mechanism
It is fixedly connected with liquid-transfering device and glass slide fetching device, control system makes spatial movement machine by control spatial movement mechanism
The motion profile of the motion parts output setting of structure makes glass slide fetching device to glass slide when biological sample tests beginning
Automatically glass slide is placed on placement platform, and make liquid-transfering device by biological sample from the sample cell of sample cell automatic sucking and from
Turn moves on on the surface of glass slide, after the completion of biological sample processing, removes glass slide automatically from glass slide placement platform；
Glass slide placement platform is mounted in sample treatment chamber body, and sample treatment cavity is enclosed by shell cavity with open topped
Chamber, glass slide placement platform is mounted at the open topped following position directly of counter sample process chambers, also for sample at
Reason cavity is specially equipped with setting temperature control equipment, humidity conditioner and air flow controller, real respectively by control system
Now to the automatic control of the inner chamber of sample treatment cavity indoor temperature, humidity and air velocity；Sample cell is provided with temperature
Regulating device, for sample cell for placing biological sample, temperature-adjusting device regulates and controls the temperature in sample cell.
As the improvement of technical solution of the present invention, system further includes label machine and barcode scanner, label machine
It connect with control system signal with barcode scanner, is swept using label of the barcode scanner to biological sample to be processed respectively
It reads, and sends scanning information to control system, the setting method and process, transmission that control system is handled according to biological sample are marked
Print command is signed to label machine, prints the label containing sample and glass slide information.
As an improvement of the above technical solution, system further includes exhaust gas adsorption device, by bracket, by exhaust gas adsorption device
It is mounted on the top of sample treatment cavity, glass slide placement platform, sample cell and biological sample drop sheet devices, is used for automatic absorbing
The pernicious gas generated in biological sample treatment process.
Above-mentioned exhaust gas adsorption device is preferably made of filter and air exhauster, during realization automatic absorbing Bioexperiment
The gas of generation is equipped with adsorbent filter layer in filter, it is preferred to use adsorbent be in soda lime, calcium lime and activated carbon
Any one or any several compositions mixture.
The spatial movement mechanism of above-mentioned biological sample drop sheet devices preferably uses XYZ three-shaft linkage device, i.e., by X-axis machine
The controlled motion mechanism composition that space is mutually orthogonal between three structure, Y-axis mechanism and Z-axis mechanism mechanism axis, X-axis mechanism, Y-axis
Mechanism and Z-axis mechanism are respectively provided with fixed part and moving portion, and wherein the fixed part of X-axis mechanism is fixedly installed in the column of bracket
On, the fixed part of Y-axis mechanism is fixedly installed in the moving portion of X-axis mechanism, and the fixed part of Z-axis mechanism is then fixedly installed in Y
In the moving portion of axis mechanism, liquid-transfering device and glass slide fetching device are fixedly installed in the moving portion of Z-axis mechanism, tri- axis of XYZ
The moving portion of each controlled motion mechanism of linkage passes through independent motor respectively and carries out corresponding space line displacement movement,
Individually under the transmission of the exclusive motor of X-axis mechanism, the fixed part of the moving portion QianYZhou mechanism of X-axis mechanism carries out side-to-side movement,
Individually under the transmission of the exclusive motor of Y-axis mechanism, the fixed part of the moving portion traction Z-axis mechanism of Y-axis mechanism carries out front and back fortune
Dynamic, individually under the transmission of the exclusive motor of Z-axis mechanism, the moving portion of Z-axis mechanism moves up and down, that is, passes through control system
Each motor of XYZ three-shaft linkage device is controlled, to control liquid-transfering device and glass slide fetching device respectively according to the space of setting
Path is mobile, to realize the automatic transporting of glass slide, placement and remove process, and realize the transfer of biological sample, draw and
Drop lets off journey.Each motor is stepper motor, direct current generator, DC speed-reducing, step-servo motor and DC servo motor.It is excellent
Step-servo motor and DC servo motor are selected, motor operation course can accurately be monitored.Sensor is photoelectric sensing
Device, Hall sensor, capacitance sensor or infrared sensor wait until, can detect to motor movement position.It is preferred that photoelectricity
Sensor, using with it is easy for installation.X-axis mechanism realizes that side-to-side movement, the realization of Y-axis mechanism move forward and backward, and Z-axis mechanism is realized up and down
Movement.
As the improvement of above-mentioned technical proposal of the present invention, preferably by the temperature control equipment and load of above-mentioned sample treatment cavity
Slide placement platform constitute sample treatment platform, wherein temperature control equipment preferably by temperature control element, radiating block, temperature-dropping fan and
Temperature sensor composition, the temperature of temperature sensor real-time detection glass slide placement platform, control system is according to temperature sensor
The detected value of temperature is compared with setting value, regulates and controls to temperature control element by the temperature signal of acquisition, realizes to glass slide
The control of the temperature of placement platform.Above-mentioned temperature control element preferably uses PTC heating element or semiconductor temperature-control element, and preferably adopts
With semiconductor temperature-control element, by the heating or cooling of temperature control element, heated up to glass slide placement platform or the control that cools down.
The humidity conditioner and air flow controller of above-mentioned sample treatment cavity are preferably by the outer of sample treatment cavity
Portion is integrated into constant humidity air-stream generating device, that is, constant humidity air-stream generating device is a closed cavity, sample treatment chamber
Body has air inlet, and the air inlet of constant humidity air-stream generating device passes through defeated with the gas outlet of constant humidity air-stream generating device
Gas flow optimized of the air-stream generating device as sample treatment cavity is arranged in air pipe connection in constant humidity air-stream generating device
Device realizes the flow control of delivery air, and air-stream generating device preferably uses blower or air pump, and constant humidity air-flow fills
Set mainly by humidification water pond, water heating elements, water temperature sensor, condenser, dehumidifying semiconductor, heat-conducting block, cooling fan and
Temperature Humidity Sensor composition, control system control water heating elements carry out heating manufacture water to the water body stored in humidification water pond and steam
Gas, water temperature sensor real-time detection humidify the water temperature in water pond, temperature detection value and setting of the control system according to water
The comparison result of value, regulates and controls water heating elements, realizes that outside air is in air-stream generating device to the accuracy controlling of water temperature
Driving under, into the inside cavity of constant humidity air-stream generating device, flow through the surface of the water body stored in humidification water pond, take
The humidified rear pipeline for entering condenser with vapor, then exsiccation region and temperature and humidity followed by dehumidifying semiconductor
The measurement point of sensor is finally flowed out from gas outlet, and into sample treatment inside cavity, cooling fan is by heat-conducting block to dehumidifying
Semiconductor is cooled down, and the heat for generating dehumidifying semiconductor successively can be discharged into environment through heat-conducting block and cooling fan, is controlled
The detected value of temperature and humidity is compared, to dehumidifying by the temperature-humidity signal that system is acquired according to Temperature Humidity Sensor with setting value
The working condition of semiconductor is regulated and controled, and realizes the humid control of the air-flow to the exsiccation region for flowing through dehumidifying semiconductor.
Above-mentioned water heating elements preferably use PTC heating element or semiconductor temperature-control element, pass through adding for water heating elements
Heat, control flow through the humidity of the air-flow of humidification water panel surface.
As the improvement of above-mentioned technical proposal of the present invention, be preferably fixedly mounted on above system bottom plate glass slide heap frame and
Glass slide layer frame, when biological sample, which is tested, to be started, control slide fetching device automatically picks up glass slide from glass slide heap frame
It takes, the open topped after carrying again by sample treatment cavity places glass slide into the inside of sample treatment cavity
To above slide platforms, and after the completion of biological sample experiment, control slide fetching device is automatically by glass slide from glass slide
It is picked up on platform, passes through the open topped of sample treatment cavity again after carrying, leave sample treatment cavity, by glass slide point
It is not placed into the corresponding layer frame of glass slide layer frame.
As the improvement of above-mentioned technical proposal of the present invention, controllable temperature sample cell is set in system backplane, controllable temperature sample
The temperature-adjusting device in pond includes temperature sensor, temperature controller, radiator and cooling blower, the sample cell case of controllable temperature sample cell
Body top is equipped with the sunk type sample well of multiple openings, biological sample is placed in sunk type sample well, temperature sensor is real-time
The internal temperature of test sample pond cabinet, the temperature signal that control system is acquired according to temperature sensor, by the detected value of temperature
It is compared with setting value, temperature controller is regulated and controled, realize the internal temperature control to sample cell cabinet.
Above-mentioned temperature controller preferably uses PTC heating element or semiconductor temperature-control element, and preferred semiconductor temperature control element passes through
The heating or cooling of temperature controller, heat up to the intracorporal sample of sample cell case or the control that cools down.
As the improvement of above-mentioned technical proposal of the present invention, pipette tips box and de- pipette tips device, biological sample are equipped in system backplane
The action part energy automatic loading and unloading that product drip the spatial movement mechanism of sheet devices is loaded pipette tips, and control system passes through control spatial movement
Mechanism, realize automatic sampling gun take arms automatically head, take off pipette tips, mixed liquid automatically and add imbibition operation, sample loading gun includes motor, piston
And pipette tips, electric machine main shaft is by driving piston up-down to carry out imbibition and filling operation.
The motor of above-mentioned sample loading gun preferably uses stepper motor, direct current generator or decelerating motor.
Above-mentioned glass slide fetching device preferably includes negative pressure pump, pressure sensor and glass slide suction nozzle, control system according to
The pressure signal of pressure sensor acquisition, the detected value of pressure is compared with setting value, is regulated and controled to negative pressure pump, is realized
The spatial movement mechanism that sheet devices include is dripped to the work method control of glass slide suction nozzle, and by linkage biological sample, is now carried
Slide takes being automatically brought into operation for piece and film releasing.
Above-mentioned control system is preferably using any one in dynamic control card, single-chip microcontroller, computer, programmable controller
Kind.
The present invention compared with prior art, has following obvious prominent substantive distinguishing features and remarkable advantage:
1. the constant humidity air-stream generating device of biological sample processing system of the present invention, can accurately control at sample
The intracorporal temperature of chamber, humidity and air velocity are managed, the samples such as peripheral blood, amniotic fluid, marrow or chorion after fixer is fixed
Pneumatic conveying drying is stabilized in sample treatment cavity of the invention, cell fixer is volatilized with speed appropriate, be can control
Chromosome spread process significantly improves metaphase chromosome number, guarantees optimal Chromosome spread degree and Chromosome spread
High consistency and repeatability；
2. the constant humidity air-stream generating device that the present invention uses, humid control precision is high, small in size, simplifies well
The internal structure of system, realizes the miniaturization of system；
3. biological sample processing box of the present invention is equipped with gas filtration absorption device, can be with decontamination of biological sample preparation procedure
The pernicious gases such as the methanol and acetic acid of middle generation greatly reduce harmful substance and break to the injury of operator and to environment
It is bad；
4. low energy consumption by the present invention, compared with similar products, energy consumption can be reduced to 500W from 1200W；
5. the XYZ three-shaft linkage that the present invention uses, the automatic drop piece operation for carrying out sample, high degree of automation, efficiency
It is high；
6. biological sample processing system of the present invention is easy to operate, low manufacture cost is had good market prospects.
Detailed description of the invention
Fig. 1 is the biological sample processing system overall appearance structural representation of the embodiment of the present invention one.
Fig. 2 is the structural schematic diagram of the sample treatment cavity of the embodiment of the present invention one.
Fig. 3 is the structural schematic diagram of the constant humidity air-stream generating device of the embodiment of the present invention three.
Fig. 4 is the structural schematic diagram of the controllable temperature sample cell of the embodiment of the present invention four.
Specific embodiment
Details are as follows for the preferred embodiment of the present invention:
Embodiment one:
In the present embodiment, it is placed referring to Fig. 1, a kind of biological sample processing system, including system backplane 7, glass slide flat
Platform 21, sample cell 6, biological sample drop sheet devices and sample treatment cavity, glass slide placement platform 21 are fixedly installed in system bottom
On plate 7, glass slide 25 is set on glass slide placement platform 21, further includes control system 15 and man-machine interface 16, control system
15 be single-chip microcontroller, and control system 15 drips sheet devices by control biological sample, biological sample to be processed is transferred to glass slide
On 25 surfaces, user realizes information exchange, man-machine interface 16 and control system 15 by man-machine interface 16 and control system 15
It is connected by communication wire, is set by parameter of the man-machine interface 16 to sample handling processes, according to specific sample
Processing requirement changes the method and process of sample treatment, by man-machine interface 16, while show sample treatment procedure information,
The status information of sensor information and associated control modules, it includes spatial movement mechanism, liquid-transfering device 8 that biological sample, which drips sheet devices,
It is fixedly connected by bracket 14 with system backplane 7 with the fixed part of glass slide fetching device 9, spatial movement mechanism, bracket 14
Bottom be fixedly installed in system backplane 7, the action part of spatial movement mechanism is picked and placed with liquid-transfering device 8 and glass slide respectively
Device 9 is fixedly connected, and control system 15 makes the motion parts of spatial movement mechanism export setting by control spatial movement mechanism
Motion profile, biological sample test start when, place glass slide fetching device 9 automatically on glass slide placement platform 21
Glass slide 25, and make liquid-transfering device 8 by biological sample from 6 automatic sucking of sample cell and be automatically transferred to the surface of glass slide 25
On, after the completion of biological sample processing, remove glass slide 25 automatically from glass slide placement platform 21；Glass slide placement platform 21
It being mounted in sample treatment cavity 2, sample treatment cavity 2 is enclosed the chamber with open topped 29 by shell cavity 28,
Glass slide placement platform 21 is mounted at the open topped following position directly of counter sample process chambers 2, is also sample treatment chamber
Body 2 is specially equipped with setting temperature control equipment, humidity conditioner and air flow controller, is realized respectively by control system 15
Automatic control to the inner chamber of sample treatment cavity 2 indoor temperature, humidity and air velocity；Sample cell 6 is provided with temperature
Regulating device, for sample cell 6 for placing biological sample, temperature-adjusting device regulates and controls the temperature in sample cell 6.
In the present embodiment, referring to Fig. 1, system further includes label machine 17 and barcode scanner 18, label machine
17 and barcode scanner 18 connect respectively with 15 signal of control system, using barcode scanner 18 to the mark of biological sample to be processed
Label carry out sweeping reading, and send scanning information to control system 15, the setting method that control system 15 is handled according to biological sample
And process, label print command is sent to label machine 17, prints the label containing sample and glass slide information.In this implementation
In example, referring to Fig. 1, label machine 17 and barcode scanner 18 are connected by controlling route with control system 15, are to control
System 15 sends and receivees instruction, is read out using label information of the barcode scanner 18 to sample to be processed, and is with control
System 15 is communicated, and according to the setting method and process of sample treatment, abstract factory printer 17 prints to be contained control system 15
The label of the information such as sample and glass slide is realized that the glass slide 25 after sample treatment is corresponding with processing sample, is avoided out
Existing mistake.
In the present embodiment, referring to Fig. 1, system further includes exhaust gas adsorption device 19, by bracket 14, exhaust gas is adsorbed and is filled
19 tops for being mounted on sample treatment cavity, glass slide placement platform 21, sample cell 6 and biological sample drop sheet devices are set, are used for
The pernicious gas generated in automatic absorption biological sample treatment process.
In the present embodiment, referring to Fig. 1, the spatial movement mechanism that biological sample drips sheet devices is XYZ three-shaft linkage device,
By space between 10 3 X-axis mechanism 3, Y-axis mechanism 4 and Z-axis mechanism mechanism axis, mutually orthogonal controlled motion mechanism is formed,
X-axis mechanism 3, Y-axis mechanism 4 and Z-axis mechanism 10 are respectively provided with fixed part and moving portion, wherein the fixed peace of the fixed part of X-axis mechanism 3
On column loaded on bracket 14, the fixed part of Y-axis mechanism 4 is fixedly installed in the moving portion of X-axis mechanism 3, and Z-axis mechanism 10
Fixed part is then fixedly installed in the moving portion of Y-axis mechanism 4, and liquid-transfering device 8 and glass slide fetching device 9 are fixedly installed in Z axis
In the moving portion of mechanism 10, the moving portion of each controlled motion mechanism of XYZ three-shaft linkage device pass through respectively independent motor into
The corresponding space line displacement movement of row, individually under the transmission of the exclusive motor of X-axis mechanism 3, the moving portion of X-axis mechanism 3 leads Y
The fixed part of axis mechanism 4 carries out side-to-side movement, individually under the transmission of the exclusive motor of Y-axis mechanism 4, the moving portion of Y-axis mechanism 4
The fixed part of traction Z-axis mechanism 10 is moved forward and backward, individually under the transmission of the exclusive motor of Z-axis mechanism 10, Z-axis mechanism 10
Moving portion move up and down, i.e., by control system 15 control XYZ three-shaft linkage device each motor, to control shifting respectively
Liquid device 8 and glass slide fetching device 9 are mobile according to the space path of setting, to realize the automatic transporting of glass slide 25, put
Process is set and removed, and realizes that journey is let off in the transfer, absorption and drop of biological sample.
In the present embodiment, referring to Fig. 1, it is fixedly arranged above bracket 14 in XYZ three-shaft linkage device, X-axis mechanism 3 is fixed on
On the column of bracket 14, one end of 4 mechanism of Y-axis is fixed in X-axis mechanism 3.Z-axis mechanism 10 is fixed in Y-axis mechanism 4.XYZ axis
Under motor drive, it is driven by screw rod, under the transmission of the exclusive motor of X-axis mechanism, Y-axis mechanism carries out left and right fortune
Dynamic, under the transmission of the exclusive motor of Y-axis mechanism, Z-axis mechanism carries out the movement of front and back, in the biography of the exclusive motor of Z-axis mechanism
Under dynamic, liquid-transfering device 8 and glass slide fetching device 9 move up and down.XYZ three-shaft linkage realizes glass slide 25 and wait locate
Sample is managed in the movement of three-dimensional space, and realizes the automation of sample handling processes.
In the present embodiment, referring to Fig. 1 and Fig. 2, glass slide heap frame 13 and glass slide layer are fixedly mounted in system backplane 7
Frame 11, when biological sample, which is tested, to be started, control slide fetching device 9 automatically picks up glass slide 25 from glass slide heap frame 13
It takes, passes through the open topped 29 of sample treatment cavity 2 again after carrying, into the inside of sample treatment cavity 2, by glass slide
25 are placed into above slide platforms 21, and after the completion of biological sample experiment, slide fetching device 9 is controlled automatically by glass slide
25 pick up from slide platforms 21, pass through the open topped 29 of sample treatment cavity 2 again after carrying, leave sample treatment
Glass slide 25 is placed individually into the corresponding layer frame of glass slide layer frame 11 by cavity 2, and by open topped 29, liquid-transfering device 8 will
25 surface of glass slide is drawn from sample cell 6 and be added to processing sample, carries out decentralized processing.
In the present embodiment, referring to Fig. 1 and Fig. 2, the temperature control equipment of sample treatment cavity 2 and glass slide placement platform
21 constitute sample treatment platform, and wherein temperature control equipment is by temperature control element 22, radiating block 23, temperature-dropping fan 24 and temperature sensing
Device 27 forms, and temperature control element 22 is installed among slide platforms 21 and radiating block 23, and 27 real-time detection of temperature sensor carries glass
The temperature of piece placement platform 21, the temperature signal that control system 15 is acquired according to temperature sensor 27, by the detected value of temperature with
Setting value is compared, and is regulated and controled to temperature control element 22, is realized according to the platform temperature of setting to glass slide placement platform 21
Temperature control.
In the present embodiment, referring to fig. 2, temperature control element 22 is semiconductor temperature-control element, passes through the heating of temperature control element 22
Or cooling, it is heated up to glass slide placement platform 21 or the control that cools down.
In the present embodiment, it is equipped with pipette tips box 5 referring to Fig. 1, in the system backplane 7 inside sample treatment cavity 2 and takes off
Pipette tips device 12, the action part energy automatic loading and unloading that biological sample drips the spatial movement mechanism of sheet devices are loaded pipette tips, control system
15, by control spatial movement mechanism, realize automatic sampling gun, head of taking arms automatically, automatically de- pipette tips and the operation of mixed liquid, sample loading gun
Including motor, piston and pipette tips, the motor of sample loading gun uses stepper motor, and electric machine main shaft is by driving piston up-down to carry out
Imbibition and filling operation.
In the present embodiment, it referring to Fig. 1, is operated according to the drop piece of chromosome in classical cytogenetics, passes through tri- axis of XYZ
Linkage, is sequentially completed following movement:
1. glass slide 25 is first taken from glass slide heap frame 13 and is put on sample treatment cavity 2 by glass slide fetching device 9
In glass slide placement platform 21 on；
2. liquid-transfering device 8 pipettes liquid feeding pipette tips；
3. the sample loading gun of liquid-transfering device 8 carries out sample mixing operation；
4. sample loading gun pipette samples；
5. the sample liquid feeding that liquid-transfering device 8 draws sample loading gun to be located at sample treatment chamber body 2 in 25 surface of glass slide；
6. the sample on 25 surface of glass slide volatilizees under conditions of setting temperature and humidity, chromosome is dispersed；
7. after sample treatment, glass slide fetching device 9 will be picked and placed containing the glass slide 25 for having handled sample to load glass
In piece layer frame 11, a sample treatment circulation is completed, then carries out new sample treatment circulate operation.
In the present embodiment, referring to Fig. 1 and Fig. 2, biological sample processing means, including constant humidity air-stream generating device 1,
Liquid-transfering device 8, glass slide fetching device 9, sample cell 6, exhaust gas adsorption device 19, XYZ three-axis moving device, barcode scanner
18, label machine 17, glass slide heap frame 13, glass slide layer frame 11, pipette tips box 5 and control system 15.Liquid-transfering device 8 is by sample
After sample blending in pipe, it is added drop-wise to 25 surface of glass slide and is handled.Glass slide fetching device 9 is by glass slide 25 from glass slide
It picks and places on heap frame 13 into sample treatment cavity 2, is handled, handled, then glass slide 25 is picked and placed and arrives glass slide layer frame 11
On.XYZ three-axis moving device controls liquid-transfering device 8 and glass slide fetching device 9 along the movement in three X-axis, Y-axis and Z axis directions
Operation.Three parts are linked, and that completes sample automatically processes process.The embodiment biological sample processing means are particularly suitable
In CYTOGENETIC ANALYSIS OF ONE, realizes the automation of chromosome drop piece process, improve analysis efficiency, guarantee the quality of sample treatment.
Barcode scanner 18 is used to scan the bar code on sample cell, and control system 15 believes the bar code information of sample cell, sample cell position
Breath, final glass slide location information printed by label machine 17, be attached on glass slide 25.
Embodiment two:
The present embodiment is basically the same as the first embodiment, and is particular in that:
In the present embodiment, exhaust gas adsorption device 19 is made of filter and air exhauster, realizes that automatic absorbing biology is real
The gas generated during testing, filter is interior to be equipped with adsorbent filter layer, and the adsorbent used is soda lime, exhaust gas adsorption device
19 are located at the top of sample processing system, are made of blower, adsorbent and shell.It is generally solid through methanol and acetic acid to handle sample
Surely the cell suspension handled is dripped containing volatile organic solvents such as methanol and acetic acid in the sample of piece processing, during processing
Volatile methanol and acetic acid escaping gas can be generated, internal system is discharged into, device itself, operator and environment are deposited
It is adversely affecting.Air containing escaping gas is pumped into exhaust gas adsorption device by the blower of exhaust gas adsorption device 19
Portion flows through the surface of adsorbent, the pernicious gases such as methanol and acetic acid in adsorbent adsorption gas flow, then gives off clean gas
Stream, realizes the purpose of air cleaning, the escaping gas that can effectively generate in adsorption sample treatment process, eliminate its to environment and
The harm of operator's health.Air-flow inside device is pumped into adsorbent equipment by blower, and filtered air-flow can be discharged to dress
Set outside.
Embodiment three:
The present embodiment is substantially the same as in the previous example, and is particular in that:
In the present embodiment, referring to Fig. 3, the humidity conditioner and air flow controller of sample treatment cavity 2 are by sample
The external harmoniousness of product process chambers 2 is constant humidity air-stream generating device 1, and constant humidity air-stream generating device 1 is one closed
Cavity, sample treatment cavity 2 have air inlet 26, the air inlet 26 and constant humidity air-flow of constant humidity air-stream generating device 1
The gas outlet 61 of generating device 1 is connected to by air delivering pipeline, and air-stream generating device is arranged in constant humidity air-stream generating device 1
60 realize the flow control of delivery air as the air flow controller of sample treatment cavity 2, and air-stream generating device 60 is air pump,
Constant humidity air-stream generating device 1 mainly by humidification water pond 51, water heating elements 53, water temperature sensor 54, condenser 55, remove
Wet semiconductor 58, heat-conducting block 56, cooling fan 57 and Temperature Humidity Sensor 59 form, and control system 15 controls water heating elements 53
Heating manufacture vapor is carried out to the water body 52 stored in humidification water pond 51,54 real-time detection of water temperature sensor humidifies water pond 51
In 52 temperature of water body, control system 15 is according to the temperature detection value of water and the comparison result of setting value, to water heating elements 53
Regulated and controled, realize the accurate control of water temperature, outside air is under the driving of air-stream generating device 60, into constant humidity air-flow
The air-flow for the specified moisture that constant humidity air-stream generating device 1 generates is imported by the inside cavity of generating device 1, the present embodiment
In sample treatment cavity 2, optimal environmental condition is provided for sample treatment, it is ensured that the processing quality of sample flows through humidification water pond
The surface of the water body 52 stored in 51 carries vapor and the humidified rear pipeline for entering condenser 55, is condensed out partial moisture,
To realize the regulation to airflow humidity, then exsiccation region and Temperature Humidity Sensor 59 followed by dehumidifying semiconductor 58
Measurement point, finally flowed out from gas outlet 61, into inside sample treatment cavity 2, cooling fan 57 is by heat-conducting block 56 to removing
Wet semiconductor 58 is cooled down, and the heat for generating dehumidifying semiconductor 58 is successively discharged into ring through heat-conducting block 56 and cooling fan 57
In border, it is ensured that the lasting normal work of the balance of heat and the semiconductor 58 that dehumidifies, control system 15 is according to Temperature Humidity Sensor 59
The Temperature and Humidity result of air-flow is sent control system 15 by the temperature-humidity signal of acquisition, by the detected value of temperature and humidity with set
Definite value is compared, and is regulated and controled to the working condition of dehumidifying semiconductor 58, is realized and is made to the dehumidifying for flowing through dehumidifying semiconductor 58
With the humid control of the air-flow in region, to accurately control the humidity of effluent stream, it is ensured that constant humidity air-stream generating device 1 produces
The humidity of raw air-flow is within the set range.
In the present embodiment, referring to Fig. 3, water heating elements 53 are semiconductor heating element, pass through water heating elements 53
Heating, control flow through the humidity of the air-flow on humidification 51 surface of water pond.Water after heating plays humidification to the air-flow for being pumped into cavity and makees
With, by airflow humidity increase to setting humidity on.
Example IV:
The present embodiment is substantially the same as in the previous example, and is particular in that:
In the present embodiment, referring to fig. 4, controllable temperature sample cell 6 is set in system backplane 7, the temperature of controllable temperature sample cell 6
Spending regulating device includes temperature sensor 69, temperature controller 67, radiator 66 and cooling blower 65, the sample of controllable temperature sample cell 6
Cabinet 68 top in pond is equipped with the sunk type sample well 70 of multiple openings, for placing biological sample to be processed, in sunk type sample
Biological sample, the temperature of the biological sample of the inside of 69 real-time detection sample cell cabinet 68 of temperature sensor, control are placed in hole 70
The temperature signal that system 15 processed is acquired according to temperature sensor 69, the detected value of temperature is compared with setting value, to temperature control
Device 67 is regulated and controled, and is realized and is controlled the temperature of sample cell cabinet 68.The heat generated in 67 course of work of temperature controller is through radiating
Body 66 and blower 65 can be discharged into environment, it is ensured that the balance of heat and the lasting normal work of temperature control element.In the present embodiment,
Referring to fig. 4, temperature controller 67 is semiconductor temperature-control element, and temperature controller 67 is installed among sample cell cabinet 68 and radiator 66, is led to
The heating or cooling for crossing temperature controller 67, heat up to the sample in sample cell cabinet 68 or the control that cools down.
Embodiment five:
The present embodiment is substantially the same as in the previous example, and is particular in that:
In the present embodiment, glass slide fetching device 9 includes negative pressure pump, pressure sensor, glass slide suction nozzle, control system
15 pressure signals acquired according to pressure sensor, the detected value of pressure is compared with setting value, is adjusted to negative pressure pump
Control realizes the work method control to glass slide suction nozzle, and drips the space that sheet devices include by linkage control biological sample and transport
Motivation structure, existing glass slide 25 take being automatically brought into operation for piece and film releasing.
The embodiment of the present invention is illustrated above in conjunction with attached drawing, but the present invention is not limited to the above embodiments, it can be with
The purpose of innovation and creation according to the present invention makes a variety of variations, under the Spirit Essence and principle of all technical solutions according to the present invention
The changes, modifications, substitutions, combinations, simplifications done should be equivalent substitute mode, as long as meeting goal of the invention of the invention, only
Otherwise the technical principle and inventive concept for deviating from biological sample processing system of the present invention, belong to protection scope of the present invention.
Claims (15)
1. a kind of biological sample processing system, including system backplane (7), glass slide placement platform (21), sample cell (6), biology
Sample drop sheet devices and sample treatment cavity, the glass slide placement platform (21) are fixedly installed on the system backplane (7),
Glass slide (25) are set on the glass slide placement platform (21), it is characterised in that: further include control system (15) and man-machine
Interface (16), the control system (15) drip sheet devices by controlling the biological sample, biological sample to be processed are shifted
Onto the glass slide (25) surface, user realizes that information is handed over by the man-machine interface (16) and the control system (15)
Mutually, the biological sample drop sheet devices include spatial movement mechanism, liquid-transfering device (8) and glass slide fetching device (9), the sky
Between the fixed part of movement mechanism be fixedly connected with the system backplane (7) by bracket (14), the bottom of the bracket (14)
Be fixedly installed on the system backplane (7), the action part of the spatial movement mechanism respectively with the liquid-transfering device (8) and
The glass slide fetching device (9) is fixedly connected, and the control system (15) is made described by controlling the spatial movement mechanism
The motion profile of the motion parts output setting of spatial movement mechanism takes the glass slide when biological sample tests beginning
It puts and places the glass slide (25) on device (9) Xiang Suoshu glass slide placement platform (21) automatically, and give birth to liquid-transfering device (8) will
Object sample is from automatic sucking in the sample cell of the sample cell (6) and is automatically transferred on the surface of the glass slide (25),
After the completion of biological sample processing, the glass slide (25) are removed automatically from the glass slide placement platform (21)；The load glass
Piece placement platform (21) is mounted in sample treatment cavity (2), and the sample treatment cavity (2) encloses shape by shell cavity (28)
At the chamber with open topped (29), the glass slide placement platform (21) is mounted on the corresponding sample treatment cavity (2)
Open topped following position directly at, also be the sample treatment cavity (2) specially be equipped with setting temperature control equipment, humidity control
Device and air flow controller processed realize the inside to the sample treatment cavity (2) by the control system (15) respectively
The automatic control of chamber indoor temperature, humidity and air velocity；The sample cell (6) is provided with temperature-adjusting device, the sample
For placing biological sample, the temperature-adjusting device regulates and controls the temperature in the sample cell (6) in product pond (6)；
Glass slide heap frame (13) and glass slide layer frame (11) are fixedly mounted on the system backplane (7), when biological sample is tested
It when beginning, controls the slide fetching device (9) and automatically picks up glass slide (25) from the glass slide heap frame (13), pass through
Again by the open topped (29) of the sample treatment cavity (2) after carrying, into the inside of the sample treatment cavity (2),
Glass slide (25) is placed into the slide platforms (21) above, and after the completion of biological sample experiment, control the slide
Fetching device (9) automatically picks up glass slide (25) from the slide platforms (21), passes through the sample again after carrying
The open topped (29) of product process chambers (2), leaves the sample treatment cavity (2), and glass slide (25) is placed individually into institute
In the corresponding layer frame for stating glass slide layer frame (11)；
When carrying out biological sample processing using biological sample processing system, it is sequentially completed following movement:
A. control glass slide fetching device (9) first takes glass slide (25) from glass slide heap frame (13) is put on sample treatment
On glass slide placement platform (21) in cavity (2)；
B. control liquid-transfering device (8) pipettes liquid feeding pipette tips；
C. the sample loading gun for controlling liquid-transfering device (8) carries out sample mixing operation；
D. sample loading gun pipette samples are controlled；
E. control liquid-transfering device (8) the sample liquid feeding of drawing sample loading gun is to the glass slide in sample treatment chamber body (2)
(25) surface；
F. the sample on glass slide (25) surface volatilizees under conditions of setting temperature and humidity, and chromosome is dispersed；
G. after sample treatment, control glass slide fetching device (9) will be arrived containing glass slide (25) pick-and-place for having handled sample
In glass slide layer frame (11), a sample treatment circulation is completed, then carries out new sample treatment circulate operation.
2. biological sample processing system according to claim 1, it is characterised in that: system further include label machine (17) and
Barcode scanner (18), the label machine (17) and the barcode scanner (18) are believed with the control system (15) respectively
Number connection, carry out sweeping reading using label of the barcode scanner (18) to biological sample to be processed, and scanning information is transmitted
It gives the control system (15), the setting method and process that the control system (15) is handled according to biological sample send label
The label machine (17) are given in print command, print the label containing sample and glass slide information.
3. biological sample processing system according to claim 1 or claim 2, it is characterised in that: system further includes exhaust gas adsorption device
(19), by the bracket (14), the exhaust gas adsorption device (19) is mounted on the sample treatment cavity, the glass slide
The top of placement platform (21), the sample cell (6) and biological sample drop sheet devices, at automatic absorption biological sample
The pernicious gas generated during reason.
4. biological sample processing system according to claim 3, it is characterised in that: the exhaust gas adsorption device (19) is by filtering
Device and air exhauster are constituted, and are realized the gas generated during automatic absorbing Bioexperiment, are equipped with adsorbent in the filter
Filter layer, the adsorbent used is the mixture of any one or any several compositions in soda lime, calcium lime and activated carbon.
5. biological sample processing system according to claim 1 or claim 2, it is characterised in that: the biological sample drop sheet devices
Spatial movement mechanism is XYZ three-shaft linkage device, by (10) three X-axis mechanism (3), Y-axis mechanism (4) and Z-axis mechanism mechanism shafts
The controlled motion mechanism composition that space is mutually orthogonal between line, the X-axis mechanism (3), the Y-axis mechanism (4) and the Z axis machine
Structure (10) is respectively provided with fixed part and moving portion, wherein the fixed part of the X-axis mechanism (3) is fixedly installed in the bracket (14)
Column on, the fixed part of the Y-axis mechanism (4) is fixedly installed in the moving portion of the X-axis mechanism (3), and the Z axis machine
The fixed part of structure (10) is then fixedly installed in the moving portion of the Y-axis mechanism (4), the liquid-transfering device (8) and the load glass
Piece fetching device (9) is fixedly installed in the moving portion of the Z-axis mechanism (10), the XYZ three-shaft linkage device it is each controllable
The moving portion of movement mechanism passes through independent motor respectively and carries out corresponding space line displacement movement, individually in the X-axis machine
Under the transmission of the exclusive motor of structure (3), the fixed part that the moving portion of the X-axis mechanism (3) leads the Y-axis mechanism (4) carries out left
Right movement, individually under the transmission of the exclusive motor of the Y-axis mechanism (4), the moving portion of the Y-axis mechanism (4) draws the Z
The fixed part of axis mechanism (10) is moved forward and backward, individually under the transmission of the exclusive motor of the Z-axis mechanism (10), the Z
The moving portion of axis mechanism (10) moves up and down, i.e., controls each of XYZ three-shaft linkage device by the control system (15)
Motor, it is mobile according to the space path of setting to control the liquid-transfering device (8) and the glass slide fetching device (9) respectively,
To realize automatic transporting, placement and the removal process of the glass slide (25), and realize the transfer, absorption and drop of biological sample
Let off journey.
6. biological sample processing system according to claim 1 or claim 2, it is characterised in that: the temperature of the sample treatment cavity (2)
It spends control device and the glass slide placement platform (21) constitutes sample treatment platform, wherein the temperature control equipment is by temperature control
Element (22), radiating block (23), temperature-dropping fan (24) and temperature sensor (27) composition, the temperature sensor (27) are examined in real time
Survey the temperature of the glass slide placement platform (21), the temperature that the control system (15) acquires according to the temperature sensor (27)
Signal is spent, the detected value of temperature is compared with setting value, the temperature control element (22) is regulated and controled, is realized to the load
The control of the temperature of slide placement platform (21).
7. biological sample processing system according to claim 6, it is characterised in that: the temperature control element (22) is PTC heating
Element or semiconductor temperature-control element, by the heating or refrigeration of the temperature control element (22), to the glass slide placement platform
(21) it is heated up or the control that cools down.
8. biological sample processing system according to claim 1 or claim 2, it is characterised in that: the sample treatment cavity (2) it is wet
Degree control device and air flow controller are filled by the external harmoniousness in the sample treatment cavity (2) for constant humidity air-flow
It sets (1), the constant humidity air-stream generating device (1) is a closed cavity, and the sample treatment cavity (2) has air inlet
(26), the air inlet (26) of the constant humidity air-stream generating device (1) and the constant humidity air-stream generating device (1) go out
Port (61) is connected to by air delivering pipeline, and air-stream generating device (60) are arranged in the constant humidity air-stream generating device (1)
The flow control of delivery air, the air-stream generating device are realized as the air flow controller of the sample treatment cavity (2)
It (60) is blower or air pump, the constant humidity air-stream generating device (1) is mainly by humidification water pond (51), water heating elements
(53), water temperature sensor (54), condenser (55), dehumidifying semiconductor (58), heat-conducting block (56), cooling fan (57) and warm and humid
Sensor (59) composition is spent, the control system (15) controls the water heating elements (53) to storage in the humidification water pond (51)
The water body (52) deposited carries out heating manufacture vapor, humidifies in water pond (51) described in water temperature sensor (54) real-time detection
Water body (52) temperature, the control system (15) is according to the temperature detection value of water and the comparison result of setting value, to the water
Heating element (53) is regulated and controled, and realization controls water temperature, driving of the outside air in the air-stream generating device (60)
Under, into the inside cavity of the constant humidity air-stream generating device (1), flow through the water body stored in humidification water pond (51)
(52) surface carries vapor and the humidified rear pipeline for entering the condenser (55), then followed by the dehumidifying half
The exsiccation region of conductor (58) and the measurement point of the Temperature Humidity Sensor (59) are finally flowed from the gas outlet (61)
Out, internal into the sample treatment cavity (2), the cooling fan (57) is by the heat-conducting block (56) to the dehumidifying half
Conductor (58) is cooled down, and the heat for generating dehumidifying semiconductor (58) is successively through the heat-conducting block (56) and the heat dissipation
Blower (57) can be discharged into environment, the temperature-humidity signal that the control system (15) acquires according to Temperature Humidity Sensor (59), will
The detected value of temperature and humidity is compared with setting value, is regulated and controled to the working condition of dehumidifying semiconductor (58), realization pair
Flow through the humid control of the air-flow in the exsiccation region of dehumidifying semiconductor (58).
9. biological sample processing system according to claim 8, it is characterised in that: the water heating elements (53) add for PTC
Thermal element or semiconductor temperature-control element, by the heating of the water heating elements (53), control flows through the humidification water pond (51)
The humidity of the air-flow on surface.
10. biological sample processing system according to claim 1 or claim 2, it is characterised in that: the sample cell (6) of controllable temperature
Be set on system backplane (7), the temperature-adjusting device of the sample cell (6) include temperature sensor (69), temperature controller (67),
Radiator (66) and cooling blower (65), sample cell cabinet (68) top of the sample cell (6) are equipped with the sinking of multiple openings
Style sample wells (70) places biological sample, temperature sensor (69) real-time detection in the sunk type sample well (70)
The internal temperature of the sample cell cabinet (68), the temperature that the control system (15) acquires according to the temperature sensor (69)
The detected value of temperature is compared by signal with setting value, is regulated and controled to the temperature controller (67), is realized to the sample cell
The internal temperature control of cabinet (68).
11. biological sample processing system according to claim 10, it is characterised in that: the temperature controller (67) is PTC heating
Element or semiconductor temperature-control element, by the heating or refrigeration of the temperature controller (67), in the sample cell cabinet (68)
Sample is heated up or is cooled down control.
12. biological sample processing system according to claim 1 or claim 2, it is characterised in that: be equipped with rifle on system backplane (7)
Head cartridge (5) and de- pipette tips device (12), the action part energy automatic loading and unloading of the spatial movement mechanism of the biological sample drop sheet devices
Sample loading gun, the control system (15) realize that automatic sampling gun is taken arms head, automatic automatically by controlling the spatial movement mechanism
De- pipette tips mix liquid and add imbibition operation, and the sample loading gun includes motor, piston and pipette tips, and the electric machine main shaft is by driving work
Plug, which moves up and down, carries out imbibition and filling operation.
13. biological sample processing system according to claim 12, it is characterised in that: the motor of the sample loading gun uses stepping
Motor, direct current generator or decelerating motor.
14. biological sample processing system according to claim 1 or claim 2, it is characterised in that: the glass slide fetching device (9)
Including negative pressure pump, pressure sensor, glass slide suction nozzle, pressure that the control system (15) acquires according to the pressure sensor
The detected value of pressure is compared by signal with setting value, is regulated and controled to the negative pressure pump, is realized to the glass slide suction nozzle
Work method control, and the spatial movement mechanism that sheet devices include, existing glass slide (25) are dripped by the biological sample of linking
Take being automatically brought into operation for piece and film releasing.
15. biological sample processing system according to claim 1 or claim 2, it is characterised in that: the control system (15) is movement
Control card, single-chip microcontroller, computer, any one in programmable controller.
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