CN104055510A - Wireless communication-based wearable rat olfactory nerve signal detection device and method - Google Patents

Abstract

The invention discloses a wearable rat olfactory nerve signal detection device and method based on wireless communication. The device includes a microwire electrode array, a front probe pin, a preamplifier, an FPC cable, and an FPC cable connector , lithium battery, first printed circuit board, second printed circuit board, computer or mobile terminal; the microwire electrode array includes nickel-chromium alloy microwire, electrode printed circuit board and epoxy glue, and the nickel-chromium alloy microwire is arranged in parallel to the electrode The front and back sides of the printed circuit board are arranged four on each side, and encapsulated by epoxy glue; the first printed circuit board includes an amplification filter circuit and a power module; the second printed circuit board includes a microcontroller and a Wi-Fi chip; the present invention The microwire electrode array can meet the in vivo detection of olfactory nerve signals in active rats; the invention uses lithium battery power supply combined with Wi-Fi technology, does not limit the range of activities of rats, has small requirements for the experimental environment, and has the advantages of simple operation and long-distance Advantages of measurement.

Description

Wearable rat olfactory nerve signal detection device and method based on wireless communication

technical field

The invention relates to the field of animal nerve signal detection, in particular to a wearable rat olfactory nerve signal detection device and method based on wireless communication.

Background technique

Recording and analyzing the electrophysiological activity of rat olfactory nervous system by using implantable olfactory and odor microelectrode array combined with signal acquisition system has a wide range of application requirements. At present, the in vivo multi-channel neural signal acquisition systems with relatively mature technology and commercialization mainly include the series of products launched by American companies such as Plexon, Blackrock Microsystems, TDT, and German Multichannel systems. There is still a certain gap in comparison. In addition, the implanted microelectrode arrays reported at home and abroad cannot meet the in vivo detection of olfactory odor. Traditional instruments use wired transmission, which greatly limits the range of activities of animals and has high requirements for the experimental environment.

Contents of the invention

The object of the present invention is to provide a wearable rat olfactory nerve signal detection device and method based on wireless communication for the deficiencies of the prior art.

The object of the present invention is achieved through the following technical solutions: a wearable rat olfactory nerve signal detection device based on wireless communication, including: microwire electrode array, pre-probe pin, pre-amplifier, FPC cable, FPC cable connector, lithium battery, first printed circuit board, second printed circuit board, computer or mobile terminal; wherein, the microwire electrode array includes nickel-chromium alloy microwire, electrode printed circuit board and epoxy glue, The nickel-chromium alloy microwires are arranged in parallel on the front and back sides of the electrode printed circuit board, four on each side, and encapsulated by epoxy glue; the first printed circuit board includes an amplification filter circuit and a power module; the second printed circuit board includes Microcontroller and Wi-Fi chip; one end of the preamplifier is connected to the nickel-chromium alloy microwire through the front probe pin and the electrode printed circuit board, and the other end is connected to the input end of the first printed circuit board through the FPC cable. The output end of the printed circuit board is connected to the second printed circuit board through the FPC cable; the lithium battery is connected to the power module of the first printed circuit board; the Wi-Fi chip communicates with the computer or mobile terminal through the wireless local area network; the first printed circuit board , the lithium battery and the second printed circuit board are arranged from bottom to top, and are fixed on the back of the rat through the animal backpack;

Described amplifying filter circuit comprises 8 groups of sub-circuits, and each group of sub-circuits all comprises successively connected power frequency notch filter, secondary amplifier, band-pass filter and output buffer, and amplifying and filtering circuit outputs 8 signals to preamplifier Perform amplification and filtering.

The power frequency trap includes 5 resistors R1-R5, 3 capacitors C1-C3 and 2 operational amplifiers U1-U2; one end of the resistor R1 is connected to one end of the capacitor C1 and then connected to the output end of the preamplifier, The other end of the resistor R1 is connected to one end of the resistor R2 and one end of the capacitor C3, the other end of the capacitor C1 is connected to one end of the capacitor C2 and one end of the resistor R3, and the other end of the resistor R2 is connected to the other end of the capacitor C2 and then connected to the operation The non-inverting input terminal of the amplifier U1, the other end of the capacitor C3 is connected to the other end of the resistor R3 and then connected to the inverting input terminal and output terminal of the operational amplifier U2; one end of the resistor R4 is connected to the inverting input terminal and output terminal of the operational amplifier U1 After being connected, it is used as the output terminal of the power frequency notch filter. The other end of the resistor R4 is connected to one end of the resistor R5 and then connected to the non-inverting input terminal of the operational amplifier U2, and the other end of the resistor R5 is connected to the power ground; the operational amplifiers U1 and U2 The positive power supply terminals are all connected to the +5V power supply, and the negative power supply terminals are all connected to the -5V power supply.

The secondary amplifier and the bandpass filter include 5 resistors R6-R10, 3 capacitors C4-C6 and 2 operational amplifiers U3-U4; one end of the capacitor C4 is connected to the output terminal of the power frequency notch filter, The other end is connected to one end of the resistor R8 and then connected to the non-inverting input terminal of the operational amplifier U3, and the other end of the resistor R8 is connected to the power ground; after the resistors R6 and R7 are connected, connected to the inverting input terminal of the operational amplifier U3, the other end of the resistor R6 One end is connected to the power ground, the other end of the resistor R7 is connected to one end of the resistor R9 and then connected to the output end of the operational amplifier U3; the other end of the resistor R9 is connected to the resistor R10 and one end of the capacitor C5, and the other end of the resistor R10 is connected to the capacitor C6 One end of the capacitor C5 is connected to the non-inverting input terminal of the operational amplifier U4, the other end of the capacitor C6 is connected to the power ground, and the other end of the capacitor C5 is connected to the output terminal and the inverting input terminal of the operational amplifier U4 as a secondary amplifier and band pass The output terminals of the filter; the positive power terminals of the operational amplifiers U3 and U4 are connected to the +5V power supply, and the negative power supply terminals are connected to the -5V power supply.

The output buffer includes two resistors R11-R12 and an operational amplifier U5; one end of the resistor R12 is connected to the output end of the secondary amplifier and the band-pass filter, and the other end is connected to one end of the resistor R11 and then connected to the non-phase of the operational amplifier U5 At the input end, the other end of the resistor R11 is connected to the +5V power supply; the positive power supply end of the operational amplifier U5 is connected to the +5V power supply, the negative power supply end is connected to the -5V power supply, and the inverting input end is connected to the output end as the output of the output buffer end.

A method for detecting rat olfactory nerve signals using the above-mentioned rat olfactory nerve signal detection device, comprising the following steps:

(1) Embed nickel-chromium alloy microwires in the olfactory bulb or olfactory cortex of rats: select male SD rats weighing 200–250 g, and fix them on a stereotaxic instrument after anesthesia; the operation is performed under sterile conditions, using a cranial drill The skull covering the olfactory bulb/olfactory cortex was abraded to expose the dorsal part of the olfactory bulb/olfactory cortex; the nichrome microwires were then slowly implanted into the olfactory nerve layer of rats using a hydraulic micro-propeller; embedding;

(2) Select unimolecular gas and natural gas to stimulate the rats; unimolecular gas is diluted with colorless and odorless mineral oil, and the concentration of unimolecular gas is 10 ^-15 ~ 10 ^-1 mol/L; the rats are placed in In a plexiglass cage with a length of 30 cm, a width of 25 cm, and a height of 30 cm, a filter paper dripped with 0.1 mL of monomolecular gas was placed next to the nasal cavity of the rat, and stayed for 5 seconds; after the odor response was obtained, fresh air was introduced into the cage and waited for 5 minutes ; Nickel-chromium alloy microwires record rat olfactory nerve signals;

(3) After the lithium battery is connected to the input terminal of the power module in the first printed circuit board, the microcontroller executes the initialization of the hardware and the network protocol stack, and establishes a TCP server. When the computer or mobile terminal establishes a wireless network with the Wi-Fi chip After connection, the microcontroller waits for instructions from the computer or mobile terminal to start data collection; the preamplifier performs preamplification on the rat olfactory nerve signal recorded by the nickel-chromium alloy microwire in step 3, and then performs power frequency on the signal in turn. Notch wave, secondary amplification, band-pass filter and output buffer processing; the digital-to-analog converter built in the microcontroller scans and converts the 8-channel analog signal input cyclically, and stores the data in the data buffer. After the buffer is full, the microcontroller The TCP protocol is used to send the data to a computer or a mobile terminal, and the computer or mobile terminal displays and records the rat olfactory nerve signal data.

The beneficial effects of the present invention are: the eight-channel microwire electrode array of the present invention can meet the in vivo detection of olfactory nerve signals of active rats; the present invention uses lithium battery power supply combined with Wi-Fi technology, and does not limit the range of activities of rats in the experiment , the requirements for the experimental environment are small, the operation is simple, and long-distance measurement can be performed; the device of the present invention has the advantages of low noise, light load, and high throughput.

Description of drawings

Fig. 1 is microwire electrode array structural diagram;

Fig. 2 is a structural diagram of a wearable active rat olfactory nerve signal detection device of the present invention;

Fig. 3 is a functional block diagram of the wearable active rat olfactory nerve signal detection device of the present invention;

Fig. 4 is a flow chart of the amplification filter circuit;

Fig. 5 is a circuit diagram of a power frequency notch filter;

Fig. 6 is a circuit diagram of a secondary amplifier and a bandpass filter;

Fig. 7 is output buffer circuit diagram;

Figure 8 is a schematic diagram of the power module;

Fig. 9 is a +5V power supply circuit diagram;

Figure 10 is a -5V power supply circuit diagram;

Figure 11 is a +3.3V power supply circuit diagram;

Fig. 12 is a schematic diagram of microcontroller and Wi-Fi chip;

Figure 13 is a circuit diagram of the embedded Wi-Fi module;

Fig. 14 is microcontroller control flowchart;

Figure 15 (a) is a schematic diagram of the olfactory nerve signals recorded when rats are stimulated by six different gases, and (b) is a graph showing the change in firing frequency of neurons when stimulated by different concentrations of carvone;

In the figure, nickel-chromium alloy microwire 1, electrode printed circuit board 2, epoxy glue 3, front probe pin 4, preamplifier 5, FPC cable 6, FPC cable connector 7, lithium battery 8, the first A printed circuit board 9 and a second printed circuit board 10 .

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

As shown in Figures 1-3, the wearable rat olfactory nerve signal detection device based on wireless communication of the present invention includes: microwire electrode array, front probe pin 4, preamplifier 5, FPC cable 6, FPC row Wire connector 7, lithium battery 8, first printed circuit board 9, second printed circuit board 10, computer or mobile terminal; among them, the surface of nickel-chromium alloy microwire 1 with a diameter of 65 μm is made of polymethyl vinyl acetate as an insulating layer , arranged in parallel on the front and back sides of the electrode printed circuit board 2, four on each side, the distance between the nickel-chromium alloy microwires 1 is 200-400 μm, and the free end is welded to the electrode printed circuit board 2 after stripping off the insulating layer Pad, and encapsulated with epoxy glue 3, the impedance of each nickel-chromium alloy microwire 1 is about 300kΩ at 1kHz; the first printed circuit board 9 includes an amplification filter circuit and a power module; the second printed circuit board 10 includes Microcontroller and Wi-Fi chip; one end of the preamplifier 5 is connected to the nickel-chromium alloy microwire 1 through the front probe pin 4 and the electrode printed circuit board 2, and the other end is connected to the first printed circuit board 9 through the FPC cable 6 The input end is connected, and the output end of the first printed circuit board 9 is connected with the second printed circuit board 10 through the FPC cable 6; the lithium battery 8 is connected with the power module of the first printed circuit board 9; the Wi-Fi chip is connected with the computer through the wireless local area network or a mobile terminal for communication; the first printed circuit board 9, the lithium battery 8 and the second printed circuit board 10 are arranged from bottom to top, and are fixed on the back of the rat through the animal backpack;

As shown in Figure 4, the amplifying and filtering circuit includes 8 groups of sub-circuits, each group of sub-circuits includes successively connected power frequency notch filters, secondary amplifiers, band-pass filters and output buffers, and the amplifying and filtering circuits are connected to the front The 8-way signals output by the amplifier 5 are amplified and filtered.

As shown in Figure 5, the power frequency notch filter adopts a double T network structure, including 5 resistors R1-R5, 3 capacitors C1-C3 and 2 operational amplifiers U1-U2; one end of the resistor R1 and the capacitor C1 One end is connected to the output end of the preamplifier 5, the other end of the resistor R1 is connected to one end of the resistor R2 and one end of the capacitor C3, the other end of the capacitor C1 is connected to one end of the capacitor C2 and one end of the resistor R3, and the resistor R2 The other end is connected to the other end of the capacitor C2 and then connected to the non-inverting input end of the operational amplifier U1, and the other end of the capacitor C3 is connected to the other end of the resistor R3 to be connected to the inverting input end and output end of the operational amplifier U2; the resistor R4 One end is connected to the inverting input terminal and output terminal of the operational amplifier U1 and then used as the output terminal of the power frequency notch filter. The other end of the resistor R4 is connected to one end of the resistor R5 and then connected to the non-inverting input terminal of the operational amplifier U2. The resistor R5 The other end is connected to the power supply ground; the positive power supply terminals of the operational amplifiers U1 and U2 are both connected to the +5V power supply, and the negative power supply terminals are both connected to the -5V power supply.

As shown in Figure 6, the two-stage amplifier and the bandpass filter include 5 resistors R6-R10, 3 capacitors C4-C6 and 2 operational amplifiers U3-U4; one end of the capacitor C4 is connected to the power frequency notch connected to the output end of the resistor, the other end is connected to one end of the resistor R8 and then connected to the non-inverting input of the operational amplifier U3, and the other end of the resistor R8 is connected to the power ground; the resistors R6 and R7 are connected to the inverting input of the operational amplifier U3 end, the other end of the resistor R6 is connected to the power ground, the other end of the resistor R7 is connected to one end of the resistor R9 and then connected to the output end of the operational amplifier U3; the other end of the resistor R9 is connected to the resistor R10 and one end of the capacitor C5, and the resistor R10 The other end of the capacitor C6 is connected to one end of the capacitor C6 and then connected to the non-inverting input terminal of the operational amplifier U4, the other end of the capacitor C6 is connected to the power ground, and the other end of the capacitor C5 is connected to the output terminal and the inverting input terminal of the operational amplifier U4. The output terminals of the secondary amplifier and the band-pass filter; the positive power terminals of the operational amplifiers U3 and U4 are connected to the +5V power supply, and the negative power supply terminals are connected to the -5V power supply.

As shown in Figure 7, the output buffer includes two resistors R11-R12 and an operational amplifier U5; one end of the resistor R12 is connected to the output end of the secondary amplifier and the band-pass filter, and the other end is connected to one end of the resistor R11 and then connected to The other end of the resistor R11 is connected to the +5V power supply; the positive power supply terminal of the operational amplifier U5 is connected to the +5V power supply, the negative power supply terminal is connected to the -5V power supply, and the inverting input terminal is connected to the output terminal. After that, it is used as the output terminal of the output buffer.

Figure 8 is a schematic diagram of the power module. The output voltage of the lithium battery is converted by DC/DC to obtain ±5V and +3.3V power supplies.

Figure 9 is a +5V power supply circuit, including lithium battery BAT, 2 resistors R13-R14, 3 capacitors C7-C9, inductor L1, TPS61200 chip U6. The negative pole of the lithium battery BAT is connected to one end of the capacitor C7 and the power ground, the positive pole is connected to the other end of the capacitor C7 and one end of the inductor L1, and then connected to pins 5, 6, 7, and 8 of the TPS61200 chip U6; the other end of the inductor L1 Connect with pin 3 of TPS61200 chip U6. Pins 0, 4, and 9 of TPS61200 chip U6 are connected to the power ground, pin 2 is the output terminal of the +5V power supply, pin 2 is connected to resistor R13 and one end of capacitor C9, pin 1 is connected to the terminal of capacitor C8 One end is connected, and pin 10 is connected to the other end of the resistor R13 and one end of the resistor R14; the other end of the capacitor C9, the other end of the resistor R14, and the other end of the capacitor C8 are connected to the power ground.

Figure 10 is a -5V power supply circuit, including 4 resistors R15-R18, 6 capacitors C10-C15, lithium battery BAT, diode D1, inductor L2, TPS63700 chip U7. The negative pole of the lithium battery BAT is connected to one end of the capacitor C11 and the power ground, the positive pole is connected to the resistor R15 and then connected to the No. 5 pin of the TPS63700 chip U7, and the other end of the resistor R15 is connected to one end of the capacitor C10 and then connected to the TPS63700 chip U7 Pins 3 and 4, the other end of the capacitor C10 is connected to the power ground; pins 0, 2, and 7 of the TPS63700 chip U7 are connected to the power ground, pin 1 is connected to one end of the capacitor C13, and pin 6 is connected to the power ground. The cathode of the diode D1 is connected to one end of the inductor L2, and the No. 8 pin is the -5V power supply output pin. The No. 8 pin is connected to the anode of the diode D1, one end of the resistor R17, one end of the resistor R18, and one end of the capacitor C15. 9 Pin No. 10 is connected to the other end of resistor R17, one end of resistor R16, and one end of capacitor C14; pin No. 10 is connected to one end of capacitor C12 and the other end of resistor R16; the other end of capacitor C14 is connected to the other end of resistor R18 , the other ends of the capacitors C12, C13, and C15 are connected to the power ground, and the other end of the inductor L2 is connected to the power ground.

Figure 11 is a +3.3V power supply circuit diagram, including a lithium battery BAT, two capacitors C16-C17, an inductor L3, and a TPS63031 chip U8. The negative electrode of the lithium battery BAT is connected to one end of the capacitor C16 and the power ground, the positive electrode is connected to the other end of the capacitor C16, and then connected to pins 5, 6, 7, and 8 of the TPS63031 chip U8, and pins 0, 3, and 9 of the TPS63031 chip U8 The pins are connected to the power ground, pin 4 is connected to one end of the inductor L3, pin 2 is connected to the other end of the inductor L3, pin 1 is the output terminal of the +3.3V power supply, pins 1 and 10 are connected to the capacitor One end of C17 is connected, and the other end of capacitor C17 is connected to the power ground.

Figure 12 is a schematic diagram of the microcontroller and Wi-Fi chip. The microcontroller STM32F205 and the Wi-Fi chip BCM43362 are integrated in the embedded Wi-Fi module EMW3161.

Figure 13 is a circuit diagram of the embedded Wi-Fi module EMW3161, including the embedded Wi-Fi module EMW3161U9, two capacitors C18-C19, and a button S1. Pins 1, 2, 3, 4, 45, 46, 47, and 48 of the embedded Wi-Fi module EMW3161U9 are analog input pins, which are respectively connected to the output terminals of 8 groups of amplification and filtering circuits, and pin 40 is connected to the capacitor C19 , one end of button S1 is connected, capacitor C19, and the other end of button S1 are connected to the power ground, pins 41 and 44 are connected to one end of capacitor C18 and then connected to the power ground, pins 42 and 43 are connected to the other end of capacitor C18 After connecting, connect +3.3V power supply, pin 49 is connected to power ground, and other pins are not connected.

Fig. 14 is a control flow chart of the microcontroller. The microcontroller receives commands from the computer or mobile terminal, starts signal collection, and sends data to the computer or mobile terminal through the wireless network.

Applying the above-mentioned wearable rat olfactory nerve signal detection device based on wireless communication to detect the method for rat olfactory nerve signal, comprising the following steps:

(1) Embed nickel-chromium alloy filaments 1 in the olfactory bulb or olfactory cortex of rats: select male SD rats weighing 200–250 g, and inject 10% chloral hydrate (injection volume 0.4ml/100g) from the abdominal cavity for anesthesia, Fix it on a stereotaxic instrument; the operation is performed under sterile conditions, using a cranial drill to grind away the skull covering the olfactory bulb/olfactory cortex, exposing the back of the olfactory bulb/olfactory cortex, and the body temperature of the rats must be maintained at a normal level during the experiment; The nickel-chromium alloy microwire 1 was slowly implanted into the rat olfactory nerve layer with a hydraulic micro-propeller; during the operation, the nickel-chromium alloy microwire 1 was connected to the rat olfactory nerve signal detection device for real-time monitoring. When there is a characteristic Spike signal, it is considered that the tip of the nickel-chromium alloy microwire 1 has entered the olfactory nerve layer, and the implantation of the nickel-chromium alloy microwire 1 is stopped at the same time, and the nickel-chromium alloy microwire 1 is embedded with dental cement;

(2) Choose six middle monomolecular gases (carvone, citral, isoamyl acetate, isobutanol, diacetyl, anisole) and natural gas (banana) to stimulate rats; Diluted with colorless and odorless mineral oil, the monomolecular gas concentration is 10 ^-15 ~ 10 ^-1 mol/L; put the rats in a plexiglass cage with a length of 30 cm, a width of 25 cm, and a height of 30 cm, and drop 0.1 mL of mono Put the filter paper of molecular gas next to the nasal cavity of the rat, and stay for 5 seconds; after getting the odor response, let fresh air into the cage, and wait for 5 minutes; nickel-chromium alloy microwire 1 records the olfactory nerve signal of the rat;

(3) After the lithium battery 8 is connected to the input end of the power module in the first printed circuit board 9, the microcontroller executes the initialization of the hardware and the network protocol stack, and establishes a TCP server. After the wireless network is connected, the microcontroller waits for instructions from the computer or mobile terminal to start data collection; after the preamplifier 5 preamplifies the rat olfactory nerve signal recorded by the nickel-chromium alloy microwire 1 in step 3, the signal is amplified. Carry out power frequency notch, secondary amplification, band-pass filtering and output buffer processing in sequence; the built-in digital-to-analog converter of the microcontroller scans and converts the 8-channel analog signal input cyclically, and stores the data in the data buffer. After the buffer is full, , the microcontroller uses the TCP protocol to send data to the computer or mobile terminal, and the computer or mobile terminal displays and records the rat olfactory nerve signal data. During the experiment, the computer or mobile terminal can close the data acquisition of the microcontroller by sending an end acquisition command to stop the experiment.

We analyzed the spike signals recorded by the rat olfactory nerve signal detection device and method of the present invention when rats were stimulated by six kinds of unimolecular odors, and found that the olfactory bulb signals recorded from the same rat had very high specificity, as shown in Figure 15 As shown in (a), different odor-evoked spike firing patterns lead to different types of neuron excitation. In addition, we also gradually reduce the odor concentration. Taking carvone as an example, when the concentration of carvone is between 10 ^-9 and 10 ^-1 mol/L, the frequency of spike release is almost unchanged, and once it is lower than 10 ^-9 mol/L , the frequency decreases rapidly, and the amplitude of the spike is also greatly reduced, as shown in Figure 15(b). Experimental results show that the wearable rat olfactory nerve signal detection device based on wireless communication of the present invention has very high sensitivity and can correctly record rat olfactory nerve signals.

Claims (5)

1. the Wearable rat olfactory nerves signal supervisory instrument based on wireless telecommunications, it is characterized in that, comprising: fibril electrode array, preposition probe contact pin (4), preamplifier (5), FPC winding displacement (6), FPC bus bar connector (7), lithium battery (8), the first printed circuit board (PCB) (9), the second printed circuit board (PCB) (10), computer or mobile terminal; Wherein, described fibril electrode array comprises nichrome microfilament (1), electrode printed circuit board (2) and epoxy glue (3), nichrome microfilament (1) is parallel to electrode printed circuit board (2) tow sides, respectively arrange four, encapsulate by epoxy glue (3) for every; Described the first printed circuit board (PCB) (9) comprises filtering and amplifying circuit and power module; The second printed circuit board (PCB) (10) comprises microcontroller and Wi-Fi chip; Preamplifier (5) one end is connected with nichrome microfilament (1) by preposition probe contact pin (4), electrode printed circuit board (2), the other end is connected with the first printed circuit board (PCB) (9) input by FPC winding displacement (6), and the first printed circuit board (PCB) (9) outfan is connected with the second printed circuit board (PCB) (10) by FPC winding displacement (6); Lithium battery (8) is connected with the power module of the first printed circuit board (PCB) (9); Wi-Fi chip communicates by WLAN and computer or mobile terminal; The first printed circuit board (PCB) (9), lithium battery (8) and the second printed circuit board (PCB) (10) are arranged from the bottom to top, are fixed on rat back by animal knapsack;

Described filtering and amplifying circuit comprises 8 groups of electronic circuits, every group of electronic circuit includes connected successively power frequency notch filter, two-stage amplifier, band filter and output buffering, and filtering and amplifying circuit carries out amplification filtering processing to 8 road signals of preamplifier (5) output.

2. the Wearable rat olfactory nerves signal supervisory instrument based on wireless telecommunications according to claim 1, is characterized in that, described power frequency notch filter comprises 5 resistance R 1-R5,3 capacitor C 1-C3 and 2 operational amplifier U1-U2; The outfan of access preamplifier (5) after one end of resistance R 1 is connected with one end of capacitor C 1, the other end of resistance R 1 is connected with one end of resistance R 2, one end of capacitor C 3, the other end of capacitor C 1 is connected with one end of capacitor C 2, one end of resistance R 3, the in-phase input end of access operational amplifier U1 after the other end of resistance R 2 is connected with the other end of capacitor C 2, inverting input and the outfan of access operational amplifier U2 after the other end of capacitor C 3 is connected with the other end of resistance R 3; After one end of resistance R 4 is connected with inverting input, the outfan of operational amplifier U1 as the outfan of power frequency notch filter, the in-phase input end of access operational amplifier U2 after the other end of resistance R 4 is connected with one end of resistance R 5 is connected the other end of resistance R 5 and power supply; The positive power source terminal of operational amplifier U1, U2 is all connected with+5V power supply, and negative power end is all connected with-5V power supply.

3. the Wearable rat olfactory nerves signal supervisory instrument based on wireless telecommunications according to claim 1, is characterized in that, described two-stage amplifier and band filter comprise 5 resistance R 6-R10,3 capacitor C 4-C6 and 2 operational amplifier U3-U4; One end of described capacitor C 4 is connected with the outfan of power frequency notch filter, and the in-phase input end of access operational amplifier U3 after the other end is connected with one end of resistance R 8 is connected the other end of resistance R 8 and power supply; The inverting input of access operational amplifier U3 after resistance R 6 is connected with R7 is connected the other end of resistance R 6 and power supply, the outfan of access operational amplifier U3 after the other end of resistance R 7 is connected with one end of resistance R 9; The other end of resistance R 9 is connected with one end of resistance R 10, capacitor C 5, the in-phase input end of access operational amplifier U4 after the other end of resistance R 10 is connected with one end of capacitor C 6, be connected the other end of capacitor C 6 and power supply, after the other end of capacitor C 5 is connected with outfan, the inverting input of operational amplifier U4 as the outfan of two-stage amplifier and band filter; The positive power source terminal of operational amplifier U3, U4 is all connected with+5V power supply, and negative power end is all connected with-5V power supply.

4. the Wearable rat olfactory nerves signal supervisory instrument based on wireless telecommunications according to claim 1, is characterized in that, described output buffering comprises 2 resistance R 11-R12 and operational amplifier U5; One end of resistance R 12 is connected with the outfan of two-stage amplifier and band filter, the in-phase input end of access operational amplifier U5 after the other end is connected with one end of resistance R 11, and the other end of resistance R 11 is connected with+5V power supply; The positive power source terminal of operational amplifier U5 is connected with+5V power supply, and negative power end is connected with-5V power supply, after inverting input is connected with outfan as the outfan of output buffering.

5. application rights requires a method for the detection of the Wearable rat olfactory nerves signal supervisory instrument based on wireless telecommunications rat olfactory nerves signal described in 1, it is characterized in that, comprises the following steps:

(1) nichrome microfilament (1) is embedded in to Rat Olfactory Bulb or smells cortex: choose the male SD rat of heavy 200 – 250g, after anesthesia, be fixed on stereotaxic instrument; Operation is carried out under aseptic condition, utilizes cranium to bore and grinds off the skull that olfactory bulb/smell cortex top covers, and exposes olfactory bulb/smell cortex back; Use subsequently hydraulic pressure micro-thruster that nichrome microfilament (1) is slowly implanted to rat olfactory nerves layer; Nichrome microfilament (1) is carried out to embedding with dentistry cement;

(2) choose unimolecule gas and natural gas stimulates rat; Unimolecule gas dilutes with the mineral oil of colorless and odorless, and unimolecule gas concentration is 10 ^-15～10 ^-1mol/L; Rat is put in to long 30cm, and wide 25cm, in the lucite cage of high 30cm, has the filter paper of 0.1mL unimolecule gas to be put in by rat nasal cavity by dripping, and stops 5s; Obtain, after abnormal smells from the patient response, in cage, passing into fresh air, and waiting for 5min; Nichrome microfilament (1) records rat olfactory nerves signal;

(3) after lithium battery (8) is connected with the input of power module in the first printed circuit board (PCB) (9), microcontroller is carried out the initialization of hardware and network protocol stack, and set up TCP server, after computer or mobile terminal and Wi-Fi chip are set up wireless network and are connected, microcontroller is waited for the instruction of computer or mobile terminal, log-on data collection; The rat olfactory nerves signal that preamplifier (5) is recorded to nichrome microfilament (1) in step 3 carries out after preposition amplification, and signal is carried out to notch filter, secondary amplification, bandpass filtering and output buffered successively; 8 channel analog signal inputs are changed in the built-in digital to analog converter scan round of microcontroller, and deposit data in data buffer zone, relief area completely after, microcontroller uses Transmission Control Protocol to send the data to computer or mobile terminal, by computer or mobile terminal to rat olfactory nerves signal data show, record.