CN104041462A - Method for regulating and controlling diapause of microplitis syntretomorpha - Google Patents
Method for regulating and controlling diapause of microplitis syntretomorpha Download PDFInfo
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- CN104041462A CN104041462A CN201410314662.6A CN201410314662A CN104041462A CN 104041462 A CN104041462 A CN 104041462A CN 201410314662 A CN201410314662 A CN 201410314662A CN 104041462 A CN104041462 A CN 104041462A
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- 230000005058 diapause Effects 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 22
- 241000500113 Microplitis Species 0.000 title claims description 19
- 230000001105 regulatory effect Effects 0.000 title abstract 2
- 230000001276 controlling effect Effects 0.000 title 1
- 241000256844 Apis mellifera Species 0.000 claims abstract description 55
- 241000985245 Spodoptera litura Species 0.000 claims abstract description 23
- 238000003860 storage Methods 0.000 claims abstract description 13
- 230000006698 induction Effects 0.000 claims abstract description 12
- 239000004576 sand Substances 0.000 claims abstract description 9
- 230000032669 eclosion Effects 0.000 claims abstract description 6
- 230000003071 parasitic effect Effects 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000500112 Microplitis sp. Species 0.000 claims description 2
- 230000033228 biological regulation Effects 0.000 abstract description 9
- 238000012360 testing method Methods 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
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- 230000006866 deterioration Effects 0.000 abstract 1
- 230000003699 hair surface Effects 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 22
- 241000607479 Yersinia pestis Species 0.000 description 11
- 235000013311 vegetables Nutrition 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 230000013011 mating Effects 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 239000000090 biomarker Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 240000001624 Espostoa lanata Species 0.000 description 2
- 235000009161 Espostoa lanata Nutrition 0.000 description 2
- 241000346285 Ostrinia furnacalis Species 0.000 description 2
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- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
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- 230000009469 supplementation Effects 0.000 description 2
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- 241000256837 Apidae Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
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- 241000700605 Viruses Species 0.000 description 1
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
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Abstract
一种调控斯氏侧沟茧蜂滞育的方法，该方法包括滞育诱导、滞育储存和滞育解除三个步骤。滞育诱导是将被斯氏测沟茧蜂寄生1d-3d的斜纹夜蛾幼虫单独分装于平底试管中，置于温度18±1℃、相对湿度70±10％、光周期L10:D14的条件下进行诱导，待寄主幼虫出茧后即得到滞育状态的蜂茧；滞育储存是将寄主幼虫移除后，在滞育茧上铺潮湿细沙，转移至6℃黑暗贮存；滞育解除是将滞育茧置于27±2℃、湿度70±10％、光周期L14:D10条件下，经历5d左右得到羽化的斯氏侧沟茧蜂。由该方法引导的斯氏测沟茧蜂滞育率可达98％以上，以滞育茧于6℃条件下保存3-5个月，羽化后的蜂在产卵量、寿命等生物学指标发面未出现退化。本方法能有效延长蜂种保存周期4-5倍，为斯氏测沟茧蜂发育的调控提供了参考依据。The invention discloses a method for regulating the diapause of Brachia schneidi, which comprises three steps of diapause induction, diapause storage and diapause release. Diapause induction is to separate the Spodoptera litura larvae parasitized by Brassina stigneri for 1d-3d into flat-bottomed test tubes, and place them in a temperature of 18±1°C, relative humidity of 70±10%, and photoperiod L10:D14. Induction under certain conditions, after the host larvae come out of the cocoon, the cocoon in the diapause state can be obtained; diapause storage is to remove the host larvae, spread moist fine sand on the diapause cocoon, and transfer to 6°C for dark storage; diapause storage The release is to place the diapause cocoon under the conditions of 27±2°C, humidity 70±10%, and photoperiod L14:D10, and after about 5 days to obtain the eclosion of B. sternii. Guided by this method, the diapause rate of the wasps stigmata can reach more than 98%, and the diapause cocoons are stored at 6°C for 3-5 months. There was no deterioration of the hair surface. The method can effectively prolong the storage period of the bee species by 4-5 times, and provides a reference basis for the regulation and control of the development of the bee wasp.
Description
Technical field
The invention belongs to the biological prevention field of agricultural insect pests control, relate to the method for a kind of Si Shi of regulation and control Microplitis Sp diapause.
Background technology
Prodenia litura is a kind of universal omnivorousness, fulminating pest, this worm can endanger 99 sections, more than 290 Plants, generation overlap is serious, wildness in recent years causes harm on China's various vegetables and economic crops, newly hatched larvae has gluttony, can be by the flower of surface crops, blade, fruit, even stem stalk is cleared off, its host is many, generation in a year is from generation to generation many, allow the harm increasing year by year of prodenia litura, add to again a large amount of frequent use of chemical pesticide, the pesticide resistance of prodenia litura is strengthened year by year, can not get effectively controlling.Therefore, on producing, in the urgent need to improving and promote the anti-control techniques of insect, utilize biological control to carry out insect protected pest controlling, can well avoid the shortcoming of traditional chemical control.
Utilizing the pest natural enemies such as parasitic wasp to carry out pest controlling to insect is a kind of important biological control of insect pests means.Parasitic wasp is the important natural enemy insect of prodenia litura, and honeybee produces ovum in pin main body, and after the hatching of honeybee ovum, larva of bee draws nutrition in pin main body, makes host can not complete normal life and goes through the phase final dead, thereby reach the object of prevention and control insect.At present, people have developed various pests natural enemy commodity, in agricultural production, insect can be controlled at below economic threshold, and control efficiency is very remarkable.The for example natural enemy of primary pest corn borer on corn---Trichogramma Ostriniae can very effectively be controlled corn borer to the causing harm of corn, and improves China's grain yield.But, also not yet there is remarkable achievement for the natural enemy product of this throughout the year multiple insect of prodenia litura.
Because natural enemy self grows, host is had to high dependency, therefore, how on the basis of artificial propagation natural enemy, growing of natural enemy population carried out to Effective Regulation, thereby in adaptation agricultural production, population fluctuation and the Harm of insect are the technical barriers that natural enemy product development is primarily captured.At occurring in nature, the breeding of natural enemy need to rely on its natural host, and in the process of artificial breeding pest natural enemy, also should fully take into account this characteristic, effectively adjusts the Population Growth relation between host and natural enemy.And the regulation and control of realization to natural enemy insect development progress, can utilize insect to be subject to external environment induction to reach this characteristic of diapause, by understanding the development characteristics of natural enemy insect, natural enemy insect is carried out to diapause regulation and control, at a large amount of timely termination of diapause in season that occur of insect, natural enemy insect is thrown in to field, cooperatively interact with parasitic wasp population under natural conditions, reach the object of insect protected; The natural enemy insect of means by diapause storage is simultaneously also a kind of desirable long-term protecting method, can solve natural enemy insect survival period shorter, expands the problem that numerous cycle grows, and extends in the prevention and control action time in field, improves control of insect efficiency.
Summary of the invention
Technical problem to be solved by this invention is: for above-mentioned prior art blank, taking prodenia litura solitary parasitism cocoon honeybee Si Shi Microplitis Sp as example, the method of a kind of Si Shi of regulation and control Microplitis Sp diapause is provided, by this method, Si Shi Microplitis Sp is carried out to diapause regulation and control, realize preservation and the rejuvenation of the Si Shi Microplitis Sp population of indoor propagation, produce the upper requirement to insect prevention and control to reach.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of regulate and control the method for Si Shi Microplitis Sp diapause, the method step is as follows:
A. diapause induction: comprise two steps of the acquisition in worm source and the induction of diapause;
The acquisition in worm source: prodenia litura is put into flat based tubes together with vegetable leaf, access than the ratio of 1:10 the queen bee that mating is good with host according to honeybee, after meeting honeybee 2h, queen bee is shifted out, put into the test tube that is ready to prodenia litura and vegetable leaf and carry out next round breeding, until the death of honeybee;
The induction of diapause: after queen bee shifts out, the above-mentioned Spodoptera litura larvae flat based tubes of taking over honeybee is carried out to independent packing, raise taking artificial feed and be placed in 27 ± 2 DEG C, photoperiod after the climatic cabinate of L14:D10 is fed 1d, 2d or 3d, proceed in batches 18 ± 1 DEG C, humidity 70 ± 10%, photoperiod is that the climatic cabinate of L10:D14 carries out diapause induction, and the state that grows of routine observation host larva obtains the cocoon of diapause status after host larva goes out cocoon;
B. diapause stores: the diapause cocoon of step a gained is proceeded in the flat based tubes that is covered with moist fine sand, be placed in the dark storage of 6 DEG C of environment; Spraying water to keep humidity every 5 days to fine sand is 70-80%;
C. diapause is removed: be that 25-29 DEG C, humidity are under the condition of 60-80%, photoperiod L14:D10 by the diapause cocoon of storage in temperature, go through 4-6 days, and bee eclosion, diapause status is removed, and obtains Si Shi Microplitis Sp.
Si Shi Microplitis Sp is one of Natural Enemies of prodenia litura, can effectively control the harm of prodenia litura to crop, promotes agricultural product quality, and its ecological benefits and economic benefit are all comparatively remarkable.Adopt method of the present invention, form with diapause cocoon can be preserved 3-5 month at 6 DEG C, degenerating does not appear in the honeybee after emergence in the biological indicator such as egg laying amount, life-span, remove the time that can control more exactly bee eclosion by diapause, can effectively extend honeybee kind retention cycle 4-5 doubly, be conducive to the production of artificial breeding honeybee.Generally speaking, utilize this method, can realize the conservation storage of Si Shi Microplitis Sp, thereby enriched the means of prodenia litura control, and then for the regulation and control of growing of pest natural enemy provide theoretical foundation, pest biological control is significant.
Embodiment
With specific embodiment, the present invention is further elaborated below.
For examination worm source
Prodenia litura and Si Shi Microplitis Sp are the population of Agricultural University Of Hunan's institute of viruses's artificial propagation, and condition of culture is 27 ± 2 DEG C, photoperiod L14:D10, relative moisture 70 ± 10%.Prodenia litura breeds with artificial feed.Si Shi Microplitis Sp adult, using 30% hydromel as nutritional supplementation, provides a large amount of 2-3 prodenia litura in age hosts to allow it carry out egg reproduction.
Test apparatus and material
Temperature and photoperiod carry out in climatic cabinate the experiment of Si Shi Microplitis Sp diapause induction, set two different environment, the breeding of insect population is carried out under 27 ± 2 DEG C, photoperiod L14:D10, relative moisture 70 ± 10% conditions, and diapause induction is carried out under 18 ± 1 DEG C, photoperiod L10:D14, relative moisture 70 ± 10% conditions.All the other materials have flat based tubes (2 × 10cm), gauze, cotton, elastic tape, 30% hydromel, artificial feed and wet sand etc.
Embodiment 1
1) experience the acquisition of diapause signal worm state: comprise that host cultivates, the cultivation of honeybee, connect three processes of honeybee.
Host cultivation: in climatic cabinate (27 ± 2 DEG C, L14:D10, relative moisture 70 ± 10%), prodenia litura connects generation and raises.Larva is raised with artificial feed, to six age mature larvas, larva is proceeded to the independent raising of flat based tubes to pupating.After adult eclosion, raise with 10% hydromel, ovum is collected in male and female pairings, and pieces of an egg are transferred to and on artificial feed, treat its hatching.
The cultivation of honeybee: taking 2-3 prodenia litura in age as host, prodenia litura is moved in flat based tubes together with vegetable leaf, put in test tube bottom the cotton balls that is moistened with 30% hydromel, access the queen bee of mating 12h with host than the ratio of 1:10 according to honeybee, connect after honeybee 2h, shift out queen bee and carry out next round and connect honeybee; Prodenia litura in flat based tubes after queen bee shifts out carries out packing raising, and its state that grows of routine observation, host larva goes out after cocoon, collect parasitic wasp cocoon, treat that parasitic wasp sprouts wings, nutritional supplementation using 30% hydromel as bee imago, by after male and female mating 12h, shifting out queen bee, to carry out follow-on expansion numerous.
Connect honeybee: a large amount of by parasitic host for obtaining, get the prodenia litura just casting off a skin to 3 ages some, with vegetable leaf (2cm
2) put into flat based tubes, 10 of every pipes put in test tube bottom the cotton balls that is moistened with 30% hydromel, access a queen bee of mating 12h, after 2h, queen bee are shifted out.This process repeats 20 honeybees.
2) diapause induction: after queen bee shifts out, prodenia litura is taken out separately and divides and install in the flat based tubes that is loaded with feed, put into the climatic cabinate of 27 ± 2 DEG C of temperature, relative moisture 70 ± 10%, photoperiod L14:D10.First host moves into (climatic cabinate that 18 ± 1 DEG C of temperature, humidity 70 ± 10%, photoperiod are L10:D14) in diapause condition after by parasitic 1d, in like manner, second batch, the 3rd crowd of host are respectively at being moved into after parasitic 2d, 3d in diapause condition, to obtain the Si Shi Microplitis Sp cocoon of diapause status.Diapause cocoon surface is light green color, is obviously different from the light brown of non-diapause cocoon.
3) storage requirement: by above-mentioned 2) in the diapause cocoon that obtains proceed in the flat based tubes that is covered with moist fine sand, be positioned over dark storage in the refrigerating chamber of 6 DEG C, spray a small amount of water to keep humidity as 70-80% every 5d to fine sand.
4) diapause is removed: to the Si Shi Microplitis Sp of diapause state, stimulate in conjunction with photoperiodic signal by temperature, termination of diapause, enters development process, and concrete measure is as follows:
The diapause state Si Shi Microplitis Sp that 6 DEG C of low temperature are preserved is placed in 27 ± 2 DEG C, the climatic cabinate of humidity 70 ± 10%, photoperiod L14:D10 again.Every day, timing was observed, about 5d left and right, and bee eclosion, diapause status is removed, and obtains the Si Shi Microplitis Sp of sprouting wings.
5) detection of diapause honeybee biological indicator
Get above-mentioned 4) the middle honeybee sprouting wings, raise with 30% hydromel, make male and female pairing 12h, again queen bee is moved into the flat based tubes that provides host and vegetable leaf, connect honeybee with host than the ratio of 1:10 according to honeybee, every 2h, queen bee is shifted out, packing host plant for rearing, carry out next round and connect honeybee, and so forth, until the death of queen bee is observed host and is gone out cocoon situation, record out cocoon number, and record honeybee survival number of days.This experiment is established contrast for non-diapause honeybee, and all the other conditions are identical, and it the results are shown in following table 1 and table 2.
Different diapause and the emergence of growing number of days honeybee of table 1
Note: will be moved in diapause environment by parasitic host after the growth number of days before immigration diapause environment represents to connect the 1st, 2 after honeybee, 3d.It is the surface color of cocoon that successful discrimination standard is induced in diapause, and light green color is diapause cocoon, is obviously different from the light brown of non-diapause cocoon.Identical letter representation there was no significant difference (p < 0.05, LSD).
The detection of table 2 diapause honeybee biological indicator
Note: will be moved in diapause environment by parasitic host after the growth number of days before immigration diapause environment represents to connect the 1st, 2 after honeybee, 3d.Identical letter representation there was no significant difference (p < 0.05, LSD).
Found out by upper table 2, although it is relatively low to move into the larva of bee diapause inductivity of the growth 3d before diapause environment, but its life-span and spawning rate are without big-difference too, can find that by contrasting non-diapause honeybee relevant data honeybee life-span, egg laying amount after diapause are not all affected, prove to there is feasibility with the diapause of the method regulation and control honeybee.
Claims (2)
1. a method that regulates and controls the diapause of Si Shi Microplitis Sp, is characterized in that, the method step is as follows:
A. diapause induction: after independent the Spodoptera litura larvae of being surveyed the parasitic 1d-3d of ditch cocoon honeybee by Si Shi point is installed in the flat based tubes that is loaded with feed, be placed under temperature 17-19 DEG C, the condition of humidity 60-80%, photoperiod L10:D14 and induce, after host larva goes out cocoon, obtain the honeybee cocoon of diapause status;
B. diapause stores: the diapause cocoon of step a gained is proceeded in the flat based tubes that is covered with moist fine sand, be placed in the dark storage of 6 DEG C of environment;
C. diapause is removed: be that 25-29 DEG C, humidity are under the condition of 60-80%, photoperiod L14:D10 by the diapause cocoon of storage in temperature, go through 4-6 days, and bee eclosion, diapause status is removed, and obtains Si Shi Microplitis Sp.
As claimed in claim 1 a kind of regulate and control the method for Si Shi Microplitis Sp diapause, it is characterized in that, after diapause cocoon proceeds to tiling and have in the flat based tubes of moist fine sand in step b, sprayed water to keep 70-80% humidity every 5 days to fine sand.
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Cited By (4)
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| CN104304193A (en) * | 2014-10-28 | 2015-01-28 | 河北省农林科学院旱作农业研究所 | Aphidoletes aphidimyza diapause induction method and application thereof to production of aphidoletes aphidimyza |
| CN105766804A (en) * | 2016-03-18 | 2016-07-20 | 中南林业科技大学 | Artificial breeding method of cotesia chilonis matsumura |
| CN105794727A (en) * | 2016-03-24 | 2016-07-27 | 镇远县醉美果业有限公司 | Osmia cocoon breaking method |
| CN109874752A (en) * | 2019-04-03 | 2019-06-14 | 中国农业科学院植物保护研究所 | Diapause regulation and storage method of a belt-long wasp |
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| CN105794727A (en) * | 2016-03-24 | 2016-07-27 | 镇远县醉美果业有限公司 | Osmia cocoon breaking method |
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| CN109874752A (en) * | 2019-04-03 | 2019-06-14 | 中国农业科学院植物保护研究所 | Diapause regulation and storage method of a belt-long wasp |
| CN109874752B (en) * | 2019-04-03 | 2021-04-09 | 中国农业科学院植物保护研究所 | Diapause regulation and storage method of a belt-long wasp |
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Application publication date: 20140917 |