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CN104012968B - Agricus blazei nutrient powder and production method thereof - Google Patents

Agricus blazei nutrient powder and production method thereof Download PDF

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Publication number
CN104012968B
CN104012968B CN201410262434.9A CN201410262434A CN104012968B CN 104012968 B CN104012968 B CN 104012968B CN 201410262434 A CN201410262434 A CN 201410262434A CN 104012968 B CN104012968 B CN 104012968B
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Prior art keywords
wheat
corn
agricus blazei
blazei
nutrient powder
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CN104012968A (en
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董建生
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SHANXI RUIZHI BIOLOGY TECHNOLOGY Co Ltd
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SHANXI RUIZHI BIOLOGY TECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Seeds, Soups, And Other Foods (AREA)

Abstract

The invention provides a kind of Agricus blazei nutrient powder and production method thereof, is with Agricus blazei solid state fermentation bacterial classification, with corn and wheat for culture medium, adopts process for solid state fermentation, makes the product by solid-state fermentation containing Mycelium in Agaricus blazei Murill and metabolin thereof.The nutrition of gained tunning and content: starch 13% ~ 16%, fat 1.8% ~ 2.5%, protein 9% ~ 11%, total phenol 4.0mg/g ~ 4.6mg/g.The present invention retains on corn and the original nutritional labeling basis of wheat basic, turn increases the active component of Agricus blazei, and make this product have adequate nutrition and form and comparatively strong anti-oxidation, total phenol content significantly increases, DPPH free radical scavenging activity 89% ~ 93%.Products obtained therefrom mouthfeel is good, aromatic flavour.

Description

Agricus blazei nutrient powder and production method thereof
Technical field
The present invention relates to food compositions, particularly utilize food compositions prepared by fungi, specifically a kind of Agricus blazei nutrient powder and production method thereof.
Background technology
Agricus blazei (Agaricusblazei) is also called Brazilian mushroom, delicious flavour, good to eat, has almond flavor, integrates cuisines, beauty treatment, medical treatment, health-care effect.In recent years, the antitumor action, immunological enhancement, antioxidation, inhibitory mutagenesis effect etc. of Agricus blazei cause people and pay close attention to greatly, are development is recommended by edible mushroom principal item from FAO (Food and Agriculture Organization of the United Nation) to the whole world.The now Agricus blazei product commercially mainly Agricus blazei Agaricus Blazei Murrill polysaccharide product doing fructification and be processed into by fructification, but Agricus blazei cultivation scale is less, limits throughput, far can not meet the demand in market, thus develop Mycelium in Agaricus blazei Murill and tunning very necessary and important.Obtain Mycelium in Agaricus blazei Murill with liquid fermentation method, equipment needed thereby is complicated, invests larger; Prepare Agricus blazei nutrient powder with it, cost is higher, is difficult to become popular food.Agricus blazei process for solid state fermentation is alternative a kind of approach.By retrieval, not yet finding with corn and wheat for primary raw material, take Agricus blazei as bacterial classification, and the adequate nutrition that has adopting process for solid state fermentation to produce is formed and compared with the Agricus blazei nutrient powder of strong anti-oxidation.
Summary of the invention
The object of the present invention is to provide and a kind ofly there is adequate nutrition to form and compared with the Agricus blazei nutrient powder of strong anti-oxidation, and a kind of preparation method of Agricus blazei nutrient powder is provided, the method ferment effect is good, fast growth, be easy to suitability for industrialized production, and gained Agricus blazei nutrient powder has adequate nutrition and forms and comparatively strong anti-oxidation.
A kind of Agricus blazei nutrient powder provided by the invention is with Agricus blazei solid state fermentation bacterial classification, with corn and wheat for culture medium, adopts process for solid state fermentation, makes the product by solid-state fermentation containing Mycelium in Agaricus blazei Murill and metabolin thereof.
The production method of a kind of Agricus blazei nutrient powder provided by the invention, comprises the steps:
1) get corn 30% ~ 50%, wheat 50% ~ 70%, by weight, rinsed clean, again attrition crushing become 2mm ~ 3mm size particles respectively, and mixing, adds water and make water content reach 40%, load polypropylene plastics pocket or vial, 0.1MPa sterilizing 60min;
2), after sterilizing, access liquid Agaricus blazei, cultivates 15 days ~ 20 days 25 DEG C ~ 28 DEG C lucifuges;
3), by cultured product by solid-state fermentation dig out, dry at 60 DEG C, pulverize, cross 0.2mm and sieve and obtain Agricus blazei nutrient powder.The Major Nutrient composition of gained Agricus blazei nutrient powder and content: starch 13% ~ 16%, fat 1.8% ~ 2.5%, protein 9% ~ 11%, total phenol 4.0mg/g ~ 4.6mg/g.
Described Agaricus blazei introduces from edible mushroom research institute of Shanghai academy of agricultural sciences, is trained liquid spawn according to a conventional method.The inoculum concentration of described Agricus blazei liquid spawn is 2.5% ~ 5%.
Be easy to make Agricus blazei nutrient powder product dissolve and digestion, can after product by solid-state fermentation to be dried, elder generation is expanded, then pulverizes.
In order to make the nutrition of Agricus blazei nutrient powder product abundanter, edible better to eat, honey element, sucrose etc. can be added in Agaricus blazei powder, further obtained sweet taste Agricus blazei nutrient powder; Add bean powder, xylitol obtains beans taste Agricus blazei nutrient powder; Add milk powder, xylitol, mix thereafter, then row granulation, drying milk taste Agricus blazei nutrient powder.
Compared with prior art advantage of the present invention:
1, the present invention is at reservation corn, on the original nutritional labeling basis of wheat, turn increase Mycelium in Agaricus blazei Murill and metabolite thereof, and make this product have adequate nutrition and form and comparatively strong anti-oxidation, total phenol content significantly increases.
2, with the corn after fragmentation, wheat grain preparation culture medium, good permeability, is beneficial to mycelial growth.After access liquid spawn, at the bottom of liquid spawn can to flow to bag from sack (or bottleneck) (or at the bottom of bottle), substantially increase the speed sending out bacterium like this.
3, the Mycelium in Agaricus blazei Murill that obtains of solid fermentation and metabolite can all utilize, and products obtained therefrom aromatic flavour, mouthfeel is good.Agricus blazei nutrient powder raw material sources are extensive, and cheap, have ready conditions and become popular nutraceutical.
4, present invention process, equipment simply, are invested little, can be accomplished scale production; Production process produces without the three disasters.
Detailed description of the invention
Embodiment 1
The granular size taking 4kg and 6kg is respectively corn and the wheat of 2mm ~ 3mm, adds water, makes water content reach 40%, be dispensed into polypropylene plastics pocket after mixing, every packed 0.8kg, 0.1MPa sterilizing 60min; When material temperature drops to 30 DEG C, the liquid Agaricus blazei of 10 days cell ages of every bag of access 20ml, after 25 ~ 28 DEG C of lucifuges cultivate 18 days, takes out in 60 DEG C of oven dry from bag by solid fermentation product, pulverize, and crosses 0.2mm sieve, obtains 9.04kg.This product colour is faint yellow, has almond flavor, and nutrition is respectively starch 14%, fat 1.8%, protein 10%, total phenol content 4.6mg/g, DPPH free radical scavenging activity 92.3%.
Embodiment 2
The granular size taking 5kg is respectively corn and the wheat of 2mm ~ 3mm, adds water, makes water content reach 40%, be dispensed in vial after mixing, every bottled 0.8kg, 0.1MPa sterilizing 60min; When material temperature drops to 30 DEG C, the Agricus blazei liquid spawn of access 25ml, after 25 ~ 28 DEG C of lucifuges cultivate 19 days, takes out solid fermentation thing from bottle, dries at 60 DEG C, and mixing is expanded, pulverizes 0.2mm sieve, and obtains 8.85kg.This powder is faint yellow, has almond flavor, and nutrition is respectively starch 16%, fat 2.2%, protein 9%, total phenol content 4.3mg/g, DPPH free radical scavenging activity 90.2%.
Embodiment 3
The granular size taking 3kg and 7kg is respectively corn and the wheat of 2mm ~ 3mm, adds water, make water content reach 40% after mixing, loads in polypropylene plastics pocket, every packed 0.8kg, 0.1MPa sterilizing 60min; Access 30ml Agricus blazei liquid spawn, cultivates after 15 days, solid fermentation product is dug out from bag for 25 ~ 28 DEG C, dry at 60 DEG C, expanded, pulverizes 0.2mm sieve, and obtains 9.07kg Agricus blazei nutrient powder.This powder is faint yellow, has almond flavor, and its nutrition of products obtained therefrom is starch 13%, fat 2.0%, protein 10.5%, total phenol content 4.0mg/g, DPPH free radical scavenging activity 89.2%.
Embodiment 4
Obtain Agricus blazei nutrient powder according to the pulp furnish of case study on implementation 1, step, in this powder, add the honey element of 1%, the sucrose of 5%, obtain sweet taste Agricus blazei nutrient powder; In this powder, add bean powder 10%, xylitol 1% obtains beans taste Agricus blazei nutrient powder.
Embodiment 5
Obtain Agricus blazei nutrient powder according to the pulp furnish of case study on implementation 2, step, in this powder, add 10% milk powder, 1% xylitol, mix thereafter, then row granulation, drying milk taste Agricus blazei nutrient powder.
The assay method that in above embodiment, Agricus blazei nutrient powder product adopts:
[1] assay method of total phenol content: adopt Folin-Ciocalteucolorimetricmethod.Sample thief dilution 0.4ml, adds the Folin-Ciocalteu reagent of 2.8ml distilled water and 0.2ml, after fully shaking up, leaves standstill a few minutes, then adds the saturated Na of 0.6ml 2cO 3solution, room temperature lucifuge leaves standstill 120min, and 760nm measures light absorption value.Take gallic acid as calibration curve, result is with the quality representation of gallic acid equivalent.
[2] mensuration of DPPH radicals scavenging effect: adopt spectrophotometer method: add DPPH reagent in sample liquid, after fully shaking up, at room temperature lucifuge places 24h, surveys light absorption value at 517nm place, then calculates DPPH free radical scavenging activity.
In product, the assay method of Major Nutrient composition adopts:
The mensuration of GB/T5009.5-2010 Protein in Food
The mensuration of fat in GB/T5009.6-2003 food
The mensuration of starch in GB/T5009.9-2008 food.

Claims (3)

1. one kind has the production method of the Agricus blazei nutrient powder compared with strong anti-oxidation function, it is characterized in that, step is: the granular size taking 4kg and 6kg is respectively corn and the wheat of 2mm ~ 3mm, add water after mixing, water content is made to reach 40%, be dispensed into polypropylene plastics pocket, every packed 0.8kg, 0.1MPa sterilizing 60min; When material temperature drops to 30 DEG C, the liquid Agaricus blazei of 10 days cell ages of every bag of access 20ml, after 25 ~ 28 DEG C of lucifuges cultivate 18 days, takes out in 60 DEG C of oven dry from bag by solid fermentation product, pulverize, and crosses 0.2mm sieve, obtains 9.04kg; This product colour is faint yellow, has almond flavor, and nutrition is respectively starch 14%, fat 1.8%, protein 10%, total phenol content 4.6mg/g, DPPH free radical scavenging activity 92.3%;
Described granular size is the corn of 2mm ~ 3mm and wheat is obtain by the following method: get corn, wheat, rinsed clean, again attrition crushing form respectively.
2. one kind has the production method of the Agricus blazei nutrient powder compared with strong anti-oxidation function, it is characterized in that, step is: the granular size taking 5kg is respectively corn and the wheat of 2mm ~ 3mm, add water after mixing, water content is made to reach 40%, be dispensed in vial, every bottled 0.8kg, 0.1MPa sterilizing 60min; When material temperature drops to 30 DEG C, the Agricus blazei liquid spawn of access 25ml, after 25 ~ 28 DEG C of lucifuges cultivate 19 days, takes out solid fermentation thing from bottle, dries at 60 DEG C, and mixing is expanded, pulverizes 0.2mm sieve, and obtains 8.85kg; This powder is faint yellow, has almond flavor, and nutrition is respectively starch 16%, fat 2.2%, protein 9%, total phenol content 4.3mg/g, DPPH free radical scavenging activity 90.2%;
Described granular size is the corn of 2mm ~ 3mm and wheat is obtain by the following method: get corn, wheat, rinsed clean, again attrition crushing form respectively.
3. one kind has the production method of the Agricus blazei nutrient powder compared with strong anti-oxidation function, it is characterized in that, step is: the granular size taking 3kg and 7kg is respectively corn and the wheat of 2mm ~ 3mm, add water after mixing, water content is made to reach 40%, load in polypropylene plastics pocket, every packed 0.8kg, 0.1MPa sterilizing 60min; Access 30ml Agricus blazei liquid spawn, cultivates after 15 days, solid fermentation product is dug out from bag for 25 ~ 28 DEG C, dry at 60 DEG C, expanded, pulverizes 0.2mm sieve, and obtains 9.07kg Agricus blazei nutrient powder; This powder is faint yellow, has almond flavor, and its nutrition of products obtained therefrom is starch 13%, fat 2.0%, protein 10.5%, total phenol content 4.0mg/g, DPPH free radical scavenging activity 89.2%;
Described granular size is the corn of 2mm ~ 3mm and wheat is obtain by the following method: get corn, wheat, rinsed clean, again attrition crushing form respectively.
CN201410262434.9A 2014-06-12 2014-06-12 Agricus blazei nutrient powder and production method thereof Active CN104012968B (en)

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CN106418164A (en) * 2016-09-30 2017-02-22 山西大学 Cereal fermented nutrition powder and production method thereof
CN110353261A (en) * 2019-08-29 2019-10-22 浙江省医学科学院 A kind of sesame young pilose antler hypha powder and its preparation method and application
CN111227080A (en) * 2020-03-10 2020-06-05 山西瑞芝生物科技有限公司 Agaricus blazei murill fermented mulberry leaf tea and production method thereof

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JP3627968B2 (en) * 1999-11-04 2005-03-09 株式会社応微研 Lactic acid fermented food and method for producing the same
CN101543285B (en) * 2009-04-30 2012-06-27 福建省农业科学院农业工程技术研究所 Wheat-bran dietary fiber composite functional fungus powder product and preparation process thereof
CN102349649A (en) * 2011-10-08 2012-02-15 杨毅 Production method of novel edible and medicinal fungi solid fermented powder

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