CN103993422B - A kind of regenerated silk fibroin/chitosan derivatives blended fiber felt and preparation method thereof - Google Patents

Abstract

The invention relates to a regenerated silk fibroin/chitosan derivative blended fiber mat and a preparation method thereof, wherein the mass percentage of chitosan derivatives in the blended fiber mat is 1 to 5%, and the rest is regenerated Silk fibroin: the breaking strength of the blended fiber mat is 1.75-2.8 MPa, and the initial modulus is 70-280 MPa. The preparation method comprises: using silkworm cocoons as raw materials to prepare an aqueous solution of regenerated silk fibroin; dissolving water-soluble chitosan derivatives in water to obtain an aqueous solution of chitosan derivatives; mixing the above two solutions in a certain proportion to prepare regenerated silk fibroin / chitosan derivatives blending aqueous solution, and then using electrospinning technology to prepare blended fiber mat. The blended fiber mat prepared by this method not only has greatly improved mechanical properties compared with pure regenerated silk fibroin fiber mat, but also has good biocompatibility, moisture absorption and moisture retention, and broad-spectrum antibacterial properties. It is suitable for textile, biological Medical special tissue scaffolds and other fields.

Description

A kind of regenerated silk fibroin/chitosan derivative blended fiber mat and preparation method thereof

technical field

The invention belongs to the technical field of blended fiber mat preparation, and relates to a regenerated silk fibroin/chitosan derivative blended fiber mat and a preparation method thereof.

Background technique

Silk fibroin is degummed from silk. As a natural material that has little inflammatory response to human body, good air permeability and good biocompatibility, it has attracted more and more attention. The regenerated silk fibroin obtained by dissolving silk fibroin can be processed into various forms of materials such as gel, film and fiber mat. According to the requirements of material use, the crystallinity of re-silk fibroin material can be changed through post-processing to control its degradation rate and mechanical properties. However, the mechanical properties of pure silk fibroin materials are relatively low, and there are defects such as increased brittleness when the moisture content is low, and a serious decrease in mechanical strength in low-humidity environments, which seriously limits its wide application.

Chitosan is a product obtained by deacetylation of chitin. Generally speaking, chitin with more than 55% of its N-acetyl group removed can be called chitosan. Chitin is widely found in the shells of arthropods such as shrimps and crabs, the carapaces of insects, and the cell walls of fungi. It is the second most abundant biological polysaccharide on earth after cellulose. The processed chitosan and chitosan derivatives have been used in textile, biomedicine and other fields due to their non-toxic side effects, non-immunogenicity, excellent biocompatibility, biodegradability, and broad-spectrum antibacterial properties. be widely used. Some chitosan derivatives such as hydroxypropyl chitosan, hydroxyethyl chitosan, carboxymethyl chitosan, carboxyethyl chitosan, etc. not only retain the original excellent properties of chitosan, but also have Good water solubility, significantly improved processability. Chitosan and some chitosan derivatives have been spun into fibers for absorbable medical sutures, eliminating the pain of stitch removal for patients; used as medical dressings, suitable for treating various wounds; can also be used for weaving and processing into Various functional products, such as health underwear, antibacterial and deodorant bedding, antibacterial medical nurse clothing, etc. However, pure chitosan and its derivative fibers have high linear density, low strength, poor physical and mechanical properties in post-spinning processing, high price, and high processing and production costs.

"Preparation of3D Fibroin/Chitosan Blend Porous Scaffold for Tissue Engineering Via a Simplified Method" published by Yuhui Ruan in 2011 involves the preparation of 3D silk fibroin/chitosan blended porous scaffolds. The tensile strength of the blended scaffold is much lower than that of the pure silk fibroin scaffold, and the tensile strength of the blended porous scaffold is lower than 0.055 MPa; with the increase of the silk fibroin content, the tensile strength of the blended scaffold first increases slightly and then decreases, The blended scaffold with 75wt% silk fibroin had the highest tensile strength, only reaching 0.045MPa. In "Genipin-crosslinked silk fibroin/hydroxybutyl chitosan nanofibrous scaffolds for tissue-engineering application" published by Kuihua Zhang in 2010, genipin crosslinked silk fibroin/hydroxybutyl chitosan nanofiber scaffolds were prepared, and the crosslinking agent was changed. Content and crosslinking time, the maximum tensile strength of the scaffold prepared in the literature is 39.5MPa, and the maximum elongation at break is 11%, but the use of additional crosslinking agent will reduce the biocompatibility of the blended scaffold and increase the preparation steps , to increase production costs. The publication number is CN103436985A Chinese invention patent, which discloses a preparation method of silk fibroin/chitosan blended nanofibers. The spinning stock solution mixed with regenerated silk fibroin formic acid solution and chitosan trifluoroacetic acid solution is used for electrostatic The silk fibroin/chitosan blend nanofiber membrane was obtained by spinning. However, this method has many steps, and the use of organic solvents is not only environmentally unfriendly, but also reduces the biocompatibility of the material, and this patent does not mention the impact of chitosan on the mechanical properties of the blended nanofiber membrane. The Chinese invention patent with the publication number CN102936794A discloses a silk fibroin/carboxymethyl chitosan/polyethylene oxide/polyethylene glycol vitamin E succinate/vitamin C sodium phosphate composite nanofiber membrane The preparation method, which uses water as a solvent, is non-toxic, non-polluting, and has good biocompatibility, but the fiber membrane prepared by this method only has appropriate mechanical properties, and this patent does not mention the effect of chitosan derivatives on blending Influence of nanofibrous membrane mechanical properties. So far, there has been no research on the preparation of regenerated silk fibroin/chitosan derivative blended fiber mats with greatly improved mechanical properties by electrospinning an aqueous solution of regenerated silk fibroin/chitosan derivatives at home and abroad. Literature reports or patents.

Contents of the invention

The purpose of the present invention is to provide a kind of regenerated silk fibroin/chitosan derivative blend fiber felt and its preparation method, the present invention is to regenerate silk fibroin and chitosan derivative blend, to obtain the New fiber mat with one-component properties. The addition of chitosan derivatives greatly improves the mechanical properties of the fiber mat. This is because the chitosan derivative molecular chain contains a large number of hydroxyl groups that can form hydrogen bonds with the amino groups on the silk fibroin chain. Chitosan first destroys the original The hydrogen bond in the silk fibroin molecule forms the hydrogen bond between the rearranged chitosan and the silk fibroin molecule. The force of the hydrogen bond is much stronger than the Van der Waals force between the molecules, which will improve the mechanical properties. Chitosan derivatives and ethanol have different mechanisms for improving the mechanical strength of the blended fiber mat. After treatment with ethanol aqueous solution, the main reason is that water molecules first increase the free volume of silk fibroin molecular chains, and ethanol then enters the fibers to promote the formation of silk fibroin. Hydrogen bonds between molecular chains.

A regenerated silk fibroin/chitosan derivative blended fiber mat of the present invention, the mass percentage of chitosan derivatives in the blended fiber mat is 1-5%, and the rest is regenerated silk fibroin; The breaking strength of the blended fiber mat is 1.75-2.8 MPa, and the initial modulus is 70-280 MPa, which are respectively 25-100% and 15-350% higher than that of the pure regenerated silk fibroin fiber mat prepared under the same conditions.

As a preferred technical solution:

A regenerated silk fibroin/chitosan derivative blended fiber mat as described above, after the blended fiber mat is soaked in an aqueous ethanol solution, its breaking strength is 4-7.5 MPa, and the initial modulus is 360-450 MPa , the breaking energy is 60-120J·Kg ^-1 , which are respectively 30-150%, 100-150%, and 50-200% higher than the pure regenerated silk fibroin fiber mat after the same post-treatment.

In the aforementioned regenerated silk fibroin/chitosan derivative blended fiber mat, the ethanol treatment refers to soaking the blended fiber mat in 80-95 vol% ethanol aqueous solution for 10-30 minutes at fixed length.

A kind of regenerated silk fibroin/chitosan derivative blending fiber felt as above, described chitosan derivative is hydroxypropyl chitosan, hydroxyethyl chitosan, carboxymethyl chitosan , one of carboxyethyl chitosan.

A regenerated silk fibroin/chitosan derivative blended fiber mat as described above, the porosity of the blended fiber mat is 65-80%, and the diameter of the blended fibers constituting the fiber mat ranges from 200nm to 2.4 nm. μm.

The present invention also provides a method for preparing a regenerated silk fibroin/chitosan derivative blended fiber mat, comprising the following steps:

(1) Using silkworm cocoons as raw materials, silkworm cocoons are degummed with 0.5% by mass percent sodium carbonate aqueous solution, and then dissolved in an aqueous lithium bromide solution with a molar concentration of 9.0 to 9.3mol/L; after the solution is centrifuged, filtered, dialyzed and concentrated, the obtained The mass percentage is 25-35% regenerated silk fibroin aqueous solution;

(2) Dissolving chitosan derivatives in deionized water to prepare an aqueous solution of chitosan derivatives with a mass percentage of 1 to 5%;

(3) Mix the regenerated silk fibroin aqueous solution with the chitosan derivative aqueous solution at a mass ratio of 100:1 to 5 of the regenerated silk fibroin/chitosan derivative to prepare the regenerated silk fibroin/chitosan Derivative blended aqueous solution;

(4) The regenerated silk fibroin/chitosan derivative blended aqueous solution prepared in step (3) is used as the spinning stock solution for electrospinning to obtain the regenerated silk fibroin/chitosan derivative blended fiber mat.

A kind of preparation method of regenerated silk fibroin protein/chitosan derivative blended fiber mat as mentioned above, the electrospinning condition in the described step (4) is: the spinning voltage is 15～30kV, and the spinning solution flow rate is 0.1~1.0mL/h, the receiving distance is 5~15cm, and the relative humidity is 45~55%.

The invention provides a regenerated silk protein and chitosan derivative blended fiber mat with natural regeneration of raw materials and degradable products and a preparation method thereof. The method is simple, easy to operate, no organic solvent is used in the whole process, and it is environmentally friendly. pollution; the resulting regenerated silk protein/chitosan derivative blend fiber mat compared with pure regenerated silk fibroin fiber mat, its breaking energy, breaking strength and initial modulus are greatly improved, and it also has antibacterial, deodorizing, Excellent properties such as antistatic, strong air permeability, good moisture absorption and heat preservation.

Beneficial effect:

The present invention uses regenerated silk fibroin and chitosan derivatives to blend, on the one hand, maintains the biocompatibility of regenerated silk fibroin, and on the other hand improves its mechanical properties, blending fiber mat breaking strength, initial modulus, The fracture energy is greatly improved, and in addition, the blended fiber mat has antibacterial effect, which is suitable for the development of functional health care clothing, and is more suitable for tissue engineering scaffolds with antibacterial or antibacterial requirements to avoid tissue infection; the present invention uses a fiber with good water solubility Chitosan derivatives, avoiding the use of organic solvents, such as formic acid, trifluoroacetic acid, hexafluoroisopropanol, etc., reduce environmental pollution, greatly reduce costs, and conform to the concept of modern green industrial production.

detailed description

The present invention will be further described below in combination with specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

Example 1

A preparation method for a regenerated silk fibroin/chitosan derivative blended fiber mat, the steps of which are:

(1) Taking silkworm cocoons as raw materials, after the silkworm cocoons are degummed with 0.5% by mass percent sodium carbonate aqueous solution, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.0mol/L; after the solution is centrifuged, filtered, dialyzed and concentrated, the mass percent is obtained 25% regenerated silk fibroin aqueous solution;

(2) dissolving hydroxypropyl chitosan in deionized water to prepare a 1% aqueous solution of hydroxypropyl chitosan;

(3) The aqueous solution of regenerated silk fibroin and the aqueous solution of hydroxypropyl chitosan are mixed according to the mass ratio of regenerated silk fibroin/hydroxypropyl chitosan to 100:1 to prepare regenerated silk fibroin/hydroxypropyl chitosan Chitosan blended aqueous solution;

(4) Using the regenerated silk fibroin/hydroxypropyl chitosan blended aqueous solution prepared in step (3) as the spinning stock solution, electrospinning is carried out to obtain the regenerated silk fibroin/hydroxypropyl chitosan derivative co- Mixed fiber felt. The electrospinning conditions are as follows: the spinning voltage is 15kV, the flow rate of the spinning solution is 0.1mL/h, the receiving distance is 5cm, and the relative humidity is 45%.

The prepared blended fiber mat has a breaking strength of 2.1 MPa and an initial modulus of 100 MPa; after the blended fiber mat is soaked in 80vol% ethanol aqueous solution for 10 minutes, its breaking strength is 5.5 MPa and its initial modulus is 360 MPa, The fracture energy is 90J·Kg ^-1 ; the porosity of the blended fiber mat is 78%, and the diameter of the blended fibers constituting the fiber mat ranges from 400nm to 1.8μm.

Example 2

A preparation method for a regenerated silk fibroin/chitosan derivative blended fiber mat, the steps of which are:

(1) Taking silkworm cocoons as raw materials, after silkworm cocoons are degummed with a 0.5% aqueous sodium carbonate solution by mass percentage, they are dissolved in an aqueous lithium bromide solution with a molar concentration of 9.1mol/L; after the solution is centrifuged, filtered, dialyzed and concentrated, the mass percent is obtained 30% aqueous solution of regenerated silk fibroin;

(2) Dissolving hydroxyethyl chitosan in deionized water to prepare a 2% aqueous solution of hydroxyethyl chitosan by mass;

(3) The aqueous solution of regenerated silk fibroin and the aqueous solution of hydroxyethyl chitosan are mixed according to the mass ratio of regenerated silk fibroin/hydroxyethyl chitosan to 100:1 to prepare regenerated silk fibroin/hydroxyethyl chitosan Chitosan blended aqueous solution;

(4) Using the regenerated silk fibroin/hydroxyethyl chitosan blended aqueous solution prepared in step (3) as the spinning stock solution, electrospinning is carried out to obtain the regenerated silk fibroin/hydroxyethyl chitosan derivative co- Mixed fiber felt. The electrospinning conditions are as follows: the spinning voltage is 20kV, the flow rate of the spinning solution is 0.3mL/h, the receiving distance is 10cm, and the relative humidity is 50%.

The fracture strength of the prepared blended fiber mat is 1.75MPa, and the initial modulus is 160MPa. After the blended fiber felt was soaked in 80vol% ethanol aqueous solution for 20min at fixed length, its breaking strength was 4MPa, the initial modulus was 380MPa, and the breaking energy was 95J·Kg ^-1 ; the porosity of the blended fiber felt was 80 %, the diameter of the blended fibers constituting the fiber mat ranges from 200 nm to 2 μm.

Example 3

A preparation method for a regenerated silk fibroin/chitosan derivative blended fiber mat, the steps of which are:

(1) Taking silkworm cocoons as raw materials, after silkworm cocoons are degummed with 0.5% aqueous sodium carbonate solution by mass percentage, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.2mol/L; after the solution is centrifuged, filtered, dialyzed and concentrated, the mass percent is obtained 33% regenerated silk fibroin aqueous solution;

(2) carboxymethyl chitosan is dissolved in deionized water to prepare a 3% carboxymethyl chitosan aqueous solution;

(3) The aqueous solution of regenerated silk fibroin and the aqueous solution of carboxymethyl chitosan are mixed according to the mass ratio of regenerated silk fibroin/carboxymethyl chitosan to 100:2 to prepare regenerated silk fibroin/carboxymethyl chitosan Chitosan blended aqueous solution;

(4) With the regenerated silk fibroin/carboxymethyl chitosan blended aqueous solution prepared in step (3) as the spinning stock solution, electrospinning is carried out to obtain the regenerated silk fibroin/carboxymethyl chitosan blended fiber felt. The electrospinning conditions are as follows: the spinning voltage is 20kV, the flow rate of the spinning solution is 0.5mL/h, the receiving distance is 15cm, and the relative humidity is 50%.

The fracture strength of the prepared blended fiber mat is 2.3MPa, and the initial modulus is 70MPa. After the blended fiber felt was soaked in 85vol% ethanol aqueous solution for 30 minutes, its breaking strength was 6.4MPa, the initial modulus was 400MPa, and the breaking energy was 105J·Kg ^-1 ; the porosity of the blended fiber felt was 75%, the diameter of the blended fibers constituting the fiber mat ranges from 400nm to 2.2μm.

Example 4

A preparation method for a regenerated silk fibroin/chitosan derivative blended fiber mat, the steps of which are:

(1) Taking silkworm cocoons as raw materials, after silkworm cocoons are degummed with 0.5% by mass percent sodium carbonate aqueous solution, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.3mol/L; after the solution is centrifuged, filtered, dialyzed and concentrated, the mass percent is obtained 35% aqueous solution of regenerated silk fibroin;

(2) Carboxyethyl chitosan is dissolved in deionized water to prepare a 5% carboxyethyl chitosan aqueous solution;

(3) Mix the regenerated silk fibroin aqueous solution with the carboxyethyl chitosan aqueous solution according to the regenerated silk fibroin/carboxyethyl chitosan mass ratio of 100:3 to prepare regenerated silk fibroin/carboxyethyl chitosan Chitosan blended aqueous solution;

(4) With the regenerated silk fibroin/carboxyethyl chitosan blended aqueous solution prepared in step (3) as the spinning stock solution, electrospinning is carried out to obtain the regenerated silk fibroin/carboxyethyl chitosan blended fiber felt. The electrospinning conditions are as follows: the spinning voltage is 25kV, the flow rate of the spinning solution is 1mL/h, the receiving distance is 10cm, and the relative humidity is 50%.

The fracture strength of the prepared blended fiber mat is 2.5MPa, and the initial modulus is 280MPa. After the blended fiber mat was soaked in 90vol% ethanol aqueous solution for 20min at fixed length, its breaking strength was 5.9MPa, the initial modulus was 450MPa, and the breaking energy was 110J·Kg ^-1 ; the porosity of the blended fiber felt was 76%, the diameter of the blended fibers constituting the fiber mat ranges from 400nm to 2.4μm.

Example 5

A preparation method for a regenerated silk fibroin/chitosan derivative blended fiber mat, the steps of which are:

(1) Taking silkworm cocoons as raw materials, after the silkworm cocoons are degummed with 0.5% by mass percent sodium carbonate aqueous solution, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.0mol/L; after the solution is centrifuged, filtered, dialyzed and concentrated, the mass percent is obtained 30% aqueous solution of regenerated silk fibroin;

(2) dissolving hydroxypropyl chitosan in deionized water to prepare an aqueous solution of 3% hydroxypropyl chitosan by mass percent;

(3) Mix the regenerated silk fibroin aqueous solution with the hydroxypropyl chitosan aqueous solution at a mass ratio of regenerated silk fibroin/hydroxypropyl chitosan of 100:2 to prepare regenerated silk fibroin/hydroxypropyl chitosan Chitosan blended aqueous solution;

(4) Using the regenerated silk fibroin/hydroxypropyl chitosan blended aqueous solution prepared in step (3) as the spinning stock solution, carry out electrospinning to obtain the regenerated silk fibroin/hydroxypropyl chitosan blended fiber felt. The electrospinning conditions are as follows: the spinning voltage is 25kV, the flow rate of the spinning solution is 1mL/h, the receiving distance is 10cm, and the relative humidity is 55%.

The fracture strength of the prepared blended fiber mat is 1.9MPa, and the initial modulus is 260MPa. After the blended fiber felt was soaked in 90vol% ethanol aqueous solution for 30 minutes, its breaking strength was 7.5MPa, the initial modulus was 420MPa, and the breaking energy was 120J·Kg ^-1 ; the porosity of the blended fiber felt was 73%, the diameter of the blended fibers constituting the fiber mat ranges from 400nm to 1.8μm.

Example 6

A preparation method for a regenerated silk fibroin/chitosan derivative blended fiber mat, the steps of which are:

(1) Taking silkworm cocoons as raw materials, after the silkworm cocoons are degummed with 0.5% by mass percent sodium carbonate aqueous solution, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.0mol/L; after the solution is centrifuged, filtered, dialyzed and concentrated, the mass percent is obtained 30% aqueous solution of regenerated silk fibroin;

(2) hydroxyethyl chitosan is dissolved in deionized water to prepare a 4% aqueous solution of hydroxyethyl chitosan;

(3) The aqueous solution of regenerated silk fibroin and the aqueous solution of hydroxyethyl chitosan are mixed according to the mass ratio of regenerated silk fibroin/hydroxyethyl chitosan to 100:3 to prepare regenerated silk fibroin/hydroxyethyl chitosan Chitosan blended aqueous solution;

(4) Using the regenerated silk fibroin/hydroxyethyl chitosan blended aqueous solution prepared in step (3) as the spinning stock solution, electrospinning is carried out to obtain the regenerated silk fibroin/hydroxyethyl chitosan blended fiber felt. The electrospinning conditions are as follows: the spinning voltage is 25kV, the flow rate of the spinning solution is 1mL/h, the receiving distance is 10cm, and the relative humidity is 50%.

The fracture strength of the prepared blended fiber mat is 2.8MPa, and the initial modulus is 150MPa. After the blended fiber felt was soaked in 90vol% ethanol aqueous solution for 30min at fixed length, its breaking strength was 7.3MPa, the initial modulus was 370MPa, and the breaking energy was 60J·Kg ^-1 ; the porosity of the blended fiber felt was 76%, the diameter of the blended fibers constituting the fiber mat ranges from 400nm to 1.6μm.

Example 7

A preparation method for a regenerated silk fibroin/chitosan derivative blended fiber mat, the steps of which are:

(1) Taking silkworm cocoons as raw materials, after the silkworm cocoons are degummed with 0.5% by mass percent sodium carbonate aqueous solution, they are dissolved in a lithium bromide aqueous solution with a molar concentration of 9.0mol/L; after the solution is centrifuged, filtered, dialyzed and concentrated, the mass percent is obtained 30% aqueous solution of regenerated silk fibroin;

(2) carboxymethyl chitosan is dissolved in deionized water to prepare a 5% carboxymethyl chitosan aqueous solution;

(3) Mix the regenerated silk fibroin aqueous solution with the carboxymethyl chitosan aqueous solution according to the regenerated silk fibroin/carboxymethyl chitosan mass ratio of 100:5 to prepare the regenerated silk fibroin/carboxymethyl chitosan Chitosan blended aqueous solution;

(4) With the regenerated silk fibroin/carboxymethyl chitosan blended aqueous solution prepared in step (3) as the spinning stock solution, electrospinning is carried out to obtain the regenerated silk fibroin/carboxymethyl chitosan blended fiber felt. The electrospinning conditions are as follows: the spinning voltage is 30kV, the flow rate of the spinning solution is 1mL/h, the receiving distance is 15cm, and the relative humidity is 50%.

The fracture strength of the prepared blended fiber mat is 1.8MPa, and the initial modulus is 210MPa. After the blended fiber felt was soaked in 95vol% ethanol aqueous solution for 30min at a fixed length, its breaking strength was 4.7MPa, its initial modulus was 420MPa, and its breaking energy was 98J·Kg ^-1 ; the porosity of the blended fiber felt was 65%, and the diameter of the blended fibers constituting the fiber mat ranges from 200nm to 1.4μm.

Claims (6)

1. a kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, is characterized in that comprising the steps：

(1) with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, molar concentration is dissolved in In for the lithium bromide water solution of 9.0～9.3mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained For 25～35% regenerated silk fibroin water solutions；

(2) chitosan derivatives are dissolved in deionized water, prepare the chitosan derivatives water that mass percent is 1～5% Solution；

(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/shitosan with the chitosan derivatives aqueous solution Derivant mass ratio is 100:1～5 mixing, prepares regenerated silk fibroin/chitosan derivatives blending aqueous solution；

(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/chitosan derivatives prepared by step (3), carries out electrostatic Spinning, that is, obtain regenerated silk fibroin/chitosan derivatives blended fiber felt；

In the blended fiber felt, the weight/mass percentage composition of chitosan derivatives is 1～5%, and remaining is regenerated silk fibroin；Institute Blended fiber felt fracture strength is stated for 1.75～2.8MPa, initial moduluss are 70～280MPa.

2. the preparation method of a kind of regenerated silk fibroin according to claim 1/chitosan derivatives blended fiber felt, its It is characterised by, in step (4), electrospinning conditions are：Spinning voltage be 15～30kV, spinning flow velocity be 0.1～ 1.0mL/h, it is 5～15cm to receive distance, and relative humidity is 45～55%.

3. the preparation method of a kind of regenerated silk fibroin according to claim 1/chitosan derivatives blended fiber felt, its Be characterised by, after the blended fiber felt is through Ethanol Treatment, its fracture strength be 4～7.5MPa, initial moduluss be 360～ 450MPa, energy to failure are 60～120J Kg^-1.

4. the preparation method of a kind of regenerated silk fibroin according to claim 3/chitosan derivatives blended fiber felt, its It is characterised by, described referring to through Ethanol Treatment is soaked in blended fiber felt fixed length 10 in 80～95vol% ethanol waters ～30min.

5. the preparation method of a kind of regenerated silk fibroin according to claim 1/chitosan derivatives blended fiber felt, its It is characterised by, described chitosan derivatives are hydroxypropyl chitosan, hydroxyethyl chitosan, carboxymethyl chitosan, carboxyethyl shell One kind in polysaccharide.

6. the preparation method of a kind of regenerated silk fibroin according to claim 1/chitosan derivatives blended fiber felt, its It is characterised by, the porosity of the blended fiber felt is 65～80%, and the scope for constituting the blended fiber diameter of fiber felt is 200nm～2.4 μm.