CN103966847B - A kind of method of plants essential oil thermal sensitive liposome finish fabric - Google Patents

Abstract

The invention discloses a kind of method of plants essential oil thermal sensitive liposome finish fabric, by mix and blend under tea tree ethereal oil, menthol and P-Cymene low temperature, obtain natural aromatic antiseptic; Natural aromatic antiseptic is dissolved in phosphate buffered solution; Natural phospholipid, phosphatid ylcholine, glycerophosphatide, phosphatidyl-ethanolamine, phosphatidylserine and phosphatidic acid are mixed, adds cholesterol, be dissolved in chloroform and pour in container, be at room temperature evaporated to chamber wall and form the homogeneous film of one deck; Being added by the phosphate buffer solution of natural aromatic antiseptic in ultrasonic water bath is covered with in the container of film, obtains thermal sensitive liposome suspension after sonic oscillation; Added by adhesive in thermal sensitive liposome suspension, stirring at room temperature, to dissolving completely, obtains thermal sensitivity fragrant antimicrobial finishing agent; By textile pad in dressing liquid, two leachings two are rolled, and last fabric utilizes desivac to carry out arrangement sizing.

Description

A kind of method for finishing fabric with plant essential oil thermosensitive liposome

technical field

The invention belongs to the field of textile clothing and medical materials, and in particular relates to a method for finishing fabrics with plant essential oil thermosensitive liposomes.

Background technique

With the change of consumption concepts and health concepts, people have new requirements for textiles, and textiles with health care effects are very popular.

At present, inorganic antibacterial agents such as nano-silver, nano-zinc oxide, nano-ceramic powder, and _TiO2 , which have been studied more, are used in cotton fabric finishing, which can obtain better antibacterial and health-care properties. However, metal ions are easily transformed into oxides or reduced to simple substances to change color, affecting the color of fabrics, especially silver ions or nano-silver particles have potential toxicity to the reproductive system and liver. Some photocatalyzed metal oxides have poor antibacterial effects on skin surfaces protected from light. In addition, the metals in the fabric cannot be transformed or enriched after entering the environment, thus possibly causing ecological imbalance. Quaternary ammonium salts also have some inherent defects, such as: easy leaching from fabrics, incompatibility with anionic surfactants, etc. DC Company has produced a silicone-based quaternary ammonium antimicrobial agent (AEM5700), which can be covalently bonded to the surface of fabrics and has long-lasting antibacterial activity. However, the antibacterial activity of such quaternary ammonium compounds can also be reduced or lost due to the adsorption of dust or dead microorganisms or the formation of complexes between positive quaternary ammonium salts and negative surfactants during repeated washings. In addition, biguanides have weak antibacterial effects on fungi and are not very resistant to light. Triclosan's resistance to bacteria has been reported in the literature and has attracted attention. But its use in fabrics and many other products is banned in many countries due to the toxic polychlorinated dioxins it produces when exposed to sunlight. In short, most organic synthetic antibacterial agents have short antibacterial cycles, poor safety and chemical stability, and are prone to microbial resistance, which limits their application range. More importantly, metals, metal oxides, and organic antibacterial agents are all produced from non-renewable resources. Under the impact of resource crisis, the manufacturing cost of these antibacterial agents will inevitably increase significantly, which is not conducive to the development of antibacterial finishing.

An ideal textile antibacterial finish not only kills harmful bacteria and prevents the spread of diseases, but also must meet three basic requirements: first, safety: the product should not be excessively toxic to humans and the environment, and should not cause skin allergies and irritations; second , compatibility: the product must have no negative impact on textile performance or appearance, and must be compatible with common textile processes; third, durability: the product should be able to withstand washing and drying, and not easily leached from the fabric. To achieve this ultimate goal, natural antimicrobials are one of the best options. Because antibacterial natural active substances can be used to produce bioactive textiles that are safe, non-toxic, environmentally friendly, and non-irritating to the skin, their application in the biofunctionalization of textile fibers is becoming more and more important. Moreover, natural antibacterial agents have a wide range of sources, large quantities and types, diverse action targets, broad antibacterial spectrum, and are not easy to produce drug resistance. Therefore, it is necessary to combine antibacterial natural active substances with modern textile finishing techniques to finish cotton fabrics, which not only helps prevent fabrics from being fouled by microorganisms, but more importantly, prevents the spread of diseases and ensures the safety and comfort of the human body. Reduced cross-infection rates in public environments. This is completely consistent with people's pursuit of health, green and environmental protection concepts, and has significant social benefits.

Tea tree essential oil is an oily extract obtained from the leaves of Melaleuca alternifolia through steam distillation, and has a special aromatic smell. Tea tree essential oil has significant antibacterial activity and broad antibacterial spectrum range, and has a strong inhibitory effect on common Gram-positive bacteria, Gram-negative bacteria and fungi. Compared with chemical antibacterial agents, it has side effects and residual The unique advantage of low toxicity, no irritation to the skin. Tea tree essential oil has the functions of purifying air, sterilizing, immunizing, antisepsis, providing cell nutrition, etc. It can be used as a safe and effective antibacterial agent for medical dressings.

Artemisia argyi essential oil is a volatile aromatic substance extracted from the leaves and stems of wormwood, extruded and solvent extracted. The main therapeutic effects of Artemisia argyi essential oil are regulating qi and blood, warming meridians, dispelling cold and dampness, relieving pain, antibacterial, anti-inflammatory, stimulating digestive glands, relieving cough and reducing phlegm, promoting circulation, replenishing qi, and improving immunity.

Menthol, organic compound, colorless needle crystal or granular. It is the main component of peppermint and peppermint essential oils, and exists in free and ester states. There are 8 isomers of menthol, and their aroma properties are different. Levomenthol has a mint aroma and has a cooling effect, racemic menthol also has a cooling effect, and other isomers have no cooling effect. It can be used as flavoring agent for toothpaste, perfume, beverage and candy. It is used as a stimulating drug in medicine, acts on the skin or mucous membrane, and has a cooling and antipruritic effect; it can be used for headache, nose, pharynx, and laryngitis when taken orally.

Among the various new liposomes studied, thermosensitive liposomes are a promising branch, which effectively utilizes the dual advantages of liposomes and thermotherapy to improve the therapeutic effect, reduce toxic side effects, and increase the number of targets. tropism. At normal ambient temperature, the liposome membrane is in a densely arranged colloidal crystal state, and it is difficult for drugs to diffuse out through the liposome membrane. When the liposome is in the environment above the membrane phase transition temperature, the local high temperature will strengthen the movement of phospholipid molecules, and the structure of the liposome membrane will change. In the loose and chaotic liquid crystal state, the drug is released.

Therefore, the combination of plant essential oils and their components through thermosensitive liposome technology can give full play to the advantages of the two, prepare antibacterial agents with good antibacterial properties, and apply them to the antibacterial finishing of fabrics, which has important application value and development prospects.

Contents of the invention

The technical problem to be solved by the present invention is to provide a method for finishing fabrics with plant essential oil thermosensitive liposomes with mint fragrance.

In order to solve the problems of the technologies described above, the technical scheme adopted in the present invention is as follows:

A method for finishing fabrics with plant essential oil thermosensitive liposomes, comprising the steps of:

(1) Mix tea tree essential oil, menthol and mugwort essential oil at low temperature to obtain a natural aromatic antibacterial agent;

(2) dissolving the natural aromatic antibacterial agent obtained in step (1) in a phosphate buffer solution;

(3) Mix natural phospholipids, phosphatidylcholine, glycerophospholipids, phosphatidylethanolamine, phosphatidylserine and phosphatidic acid, add cholesterol, dissolve it in chloroform and pour it into a container (such as a round-bottomed flask), at room temperature Evaporate down to the container wall to form a uniform film;

(4) adding the phosphate buffer solution of the natural aromatic antibacterial agent obtained in step (2) into the film-covered container obtained in step (3) in an ultrasonic water bath, and obtaining a heat-sensitive liposome suspension after ultrasonic oscillation;

(5) Add the adhesive to the heat-sensitive liposome suspension obtained in step (4), stir at room temperature until completely dissolved, and prepare a heat-sensitive aromatic antibacterial finishing agent;

(6) The fabric is dipped in the finishing solution, two dips and two pads, and finally the fabric is finished and shaped by freeze-drying.

In step (1), the mass ratio of the tea tree essential oil, menthol and mugwort essential oil is 1-3.5:1:1-3, the stirring temperature is 0-4°C, and the stirring speed is 30-120 rpm.

In step (2), the phosphate buffer solution was prepared as follows: sodium chloride 8g/L, potassium chloride 0.2g/L, disodium hydrogen phosphate 1.44g/L, dipotassium hydrogen phosphate 0.24g/L, solvent For water, adjust the pH value to 6.5 with hydrochloric acid, and add Tween 80 with a final concentration of 6v/v%.

In step (2), the natural aromatic antibacterial agent is added to the phosphate buffer solution at a concentration of 15-30 g/L.

In step (3), the natural phospholipid is selected from soybean lecithin or egg yolk lecithin; the phosphatidylcholine is selected from 1-myristoyl-2-stearoyl lecithin, dipalmitoyl lecithin, Distearoyl lecithin or 1-palmitoyl-2-stearoyl lecithin; the glycerophospholipid is selected from dipalmitoylphosphatidylglycerol sodium salt or distearoylphosphatidylglycerol sodium salt; the phospholipid Acylethanolamine is selected from dimyristoylphosphatidylethanolamine, dipalmitoylphosphatidylethanolamine or distearoylphosphatidylethanolamine; described phosphatidylserine is selected from dipalmitoylphosphatidylserine sodium salt or distearoylphosphatidylethanolamine Serine sodium salt; the phosphatidic acid is selected from dimyristoyl phosphatidic acid sodium salt, dipalmitoyl phosphatidic acid sodium salt or dimyristoyl phosphatidic acid sodium salt; the natural phospholipids, phosphatidylcholine, glycerophospholipid , phosphatidylethanolamine, phosphatidylserine and phosphatidic acid are mixed in a ratio of 1:1:1:1:1:1; natural phospholipids, phosphatidylcholine, glycerophospholipids, phosphatidylethanolamine, phosphatidylserine and The mass ratio of phosphatidic acid mixture to cholesterol is 4-9:1.

In step (4), preheat the phosphate buffer solution containing the natural aromatic antibacterial agent to 55°C in an ultrasonic water bath at 55°C, put it into a container covered with a film, and vibrate ultrasonically for 10 to 50 minutes to obtain a thermosensitive liposome suspension. liquid, the ultrasonic frequency is 400MHz, and the oscillation frequency is 200 rpm; the mass ratio of the natural aromatic antibacterial agent to the film is 3:2-1. The mass of the film can be calculated according to the total mass of phospholipid mixture and cholesterol.

In step (5), the adhesive is selected from starch, chitosan, sodium alginate, carboxymethyl cellulose, dextrin or polyvinyl alcohol; the amount of the adhesive relative to the thermosensitive liposome suspension is 15 ~30g/L.

In step (6), the fabric is dipped in the finishing solution at a bath ratio (that is, the mass ratio of the finishing solution to the fabric) of 3 to 5:1, two dipping and two rolling, the roll pressure is 0.1 to 0.25 MPa, and the excess rate 80-100%.

In step (6), the procedure of the freeze-drying method is as follows: first, drop from room temperature to -80°C at a rate of 1°C/min, then turn on the vacuum pump for freeze-drying, the vacuum degree is less than 13Pa, and maintain at -80°C for 24h, Then raise the temperature to -55°C at a rate of 0.5°C/min, inject nitrogen gas into the freeze-drying chamber to return the air pressure to 1 atmosphere, return to room temperature at a natural heating rate, and take out the freeze-dried fabric.

The fabric prepared by the above method is also within the protection scope of the present invention. This position can prepare medical materials, antibacterial materials, textile and clothing materials, etc., which are widely used in biomedicine, textile industry, medical antibacterial health care and other fields.

Beneficial effect: compared with the prior art, the preparation method of the present invention has the following advantages:

1. The fabric finished by the present invention realizes the function of "smart switch" at 35-40°C. On the one hand, it can automatically adjust the release of essential oil components according to the temperature, and realize targeted antibacterial in the process of wearing and taking. It can be used in a very short time. Quickly kill microorganisms within a short period of time and improve antibacterial efficiency; on the other hand, the fabric can take away part of the body surface heat when the essential oil components volatilize, and the body surface has a higher temperature and more heat dissipation, and its cooling effect is more obvious .

2. Compared with other antibacterial finishes, the present invention uses natural antibacterial agents and adhesives with good biocompatibility to form an aromatic antibacterial finishing agent. A good new finishing agent that can form a film on the surface of the fabric, which reduces the adverse effects of chemical reagents on human skin.

3. Combining the advantages of both plant essential oils and liposomes, combined with freeze-drying and solidification, the essential oil liposomes are embedded in the fibers without destroying the liposome structure, making the essential oil liposomes more stable, with strong antibacterial activity and heat The sensitive release effect is obvious, and the broad-spectrum antibacterial effect is remarkable;

4. The prepared fabric has a certain mint fragrance, which can optimize the environment to a certain extent, purify the air, and can also accelerate the elimination of people's nervous tension, relieve pressure, delight the mood, and satisfy people's spiritual enjoyment;

5. It has a wide range of uses, and can be used as medical materials, antibacterial materials, textile and clothing materials, health care, antibacterial care, etc.

Description of drawings

Figure 1 Curve of menthol content in fabrics as a function of time.

Fig. 2 Determination curves of the release characteristics of fabric heat-sensitive essential oils.

Detailed ways

The present invention can be better understood from the following examples. However, those skilled in the art can easily understand that the content described in the embodiments is only for illustrating the present invention, and should not and will not limit the present invention described in the claims.

Example 1:

A method for finishing fabrics with plant essential oil thermosensitive liposomes, comprising the steps of:

(1) Mix tea tree essential oil, menthol and mugwort essential oil at a mass ratio of 1:1:1 at 0°C with a stirring speed of 30 rpm to obtain a natural aromatic antibacterial agent.

(2) Prepare phosphate buffer solution: sodium chloride 8g/L, potassium chloride 0.2g/L, disodium hydrogen phosphate 1.44g/L, dipotassium hydrogen phosphate 0.24g/L, adjust pH to 6.5 with hydrochloric acid, add The final concentration was 6% v/v Tween 80. Dissolve the natural fragrance antibacterial agent in the above-mentioned phosphate buffer solution, so that the concentration of the natural fragrance antibacterial agent is 15g/L.

(3) Soybean lecithin, 1-myristoyl-2-stearoyl lecithin, dipalmitoylphosphatidylglycerol sodium salt, dimyristoylphosphatidylethanolamine, dipalmitoylphosphatidylserine sodium salt and dimeat The sodium myristoyl phosphatidic acid is mixed in a ratio of 1:1:1:1:1:1. Take 100 mg of phospholipid mixture and 25 mg of cholesterol, dissolve in chloroform and pour into a round bottom flask, and rotate at room temperature until a uniform film is formed on the wall of the bottle.

(4) Preheat the phosphate buffer solution containing natural antibacterial agents to 55°C in an ultrasonic water bath at 55°C, add it into a round-bottomed flask covered with a film, and oscillate ultrasonically for 10 minutes at a frequency of 400MHz and an oscillation frequency of 200 rev/min, the mass ratio of natural aromatic antibacterial agent (excluding the mass of phosphate buffer solution) to film is 3:1, and a heat-sensitive liposome suspension is prepared.

(5) Add dextrin as an adhesive to the heat-sensitive liposome suspension at a concentration of 15 g/L, stir at room temperature until completely dissolved, and obtain a heat-sensitive aromatic antibacterial finishing agent;

(6) Pad the fabric in the finishing solution with a bath ratio of 3:1, two dipping and two rolling, the roll pressure is 0.1MPa, and the excess rate is 80%; finally, the fabric drops from room temperature at a rate of 1°C/min to -80°C, then turn on the vacuum pump for freeze-drying, the vacuum degree is less than 13Pa, maintain at -80°C for 24 hours, then raise the temperature to -55°C at a rate of 0.5°C/min, inject nitrogen into the freeze-drying chamber to return the air pressure to 1 atmosphere Return to room temperature at a natural heating rate, and take out the freeze-dried fabric.

Example 2:

A method for finishing fabrics with plant essential oil thermosensitive liposomes, comprising the steps of:

(1) Mix tea tree essential oil, menthol and mugwort essential oil at a mass ratio of 3.5:1:3 at 4°C with a stirring speed of 120 rpm to obtain a natural aromatic antibacterial agent;

(2) Prepare phosphate buffer solution: sodium chloride 8g/L, potassium chloride 0.2g/L, disodium hydrogen phosphate 1.44g/L, dipotassium hydrogen phosphate 0.24g/L, adjust pH to 6.5 with hydrochloric acid, add The final concentration was 6% v/v Tween 80. Dissolve the natural fragrance antibacterial agent in the above-mentioned phosphate buffer solution, so that the concentration of the natural fragrance antibacterial agent is 30g/L.

(3) Egg yolk lecithin, dipalmitoyl lecithin, distearoylphosphatidylglycerol sodium salt, dipalmitoylphosphatidylethanolamine, distearoylphosphatidylserine sodium salt and dipalmitoylphosphatidic acid sodium salt as substances The volume ratio is 1:1:1:1:1:1 for mixing. Take 100 mg of phospholipid mixture and 11.11 mg of cholesterol, dissolve it in chloroform and pour it into a round bottom flask, and spin evaporate at room temperature until a uniform film is formed on the wall of the bottle.

(4) Preheat the phosphate buffer solution containing natural antibacterial agents to 55°C in an ultrasonic water bath at 55°C, add it to a round-bottomed flask covered with a film, and oscillate ultrasonically for 50 minutes at a frequency of 400MHz and 200 rpm /min, the mass ratio of natural aromatic antibacterial agent (not including the quality of phosphate buffer solution) to film is 3:2, and the thermosensitive liposome suspension is prepared.

(5) Add polyvinyl alcohol as an adhesive to the heat-sensitive liposome suspension at a concentration of 30 g/L, stir at room temperature until completely dissolved, and obtain a heat-sensitive aromatic antibacterial finishing agent;

(6) Pad the fabric in the finishing solution with a bath ratio of 5:1, two dipping and two rolling, the roll pressure is 0.25MPa, and the excess rate is 100%; finally, the fabric drops from room temperature at a rate of 1°C/min to -80°C, then turn on the vacuum pump for freeze-drying, the vacuum degree is less than 13Pa, maintain at -80°C for 24 hours, then raise the temperature to -55°C at a rate of 0.5°C/min, inject nitrogen into the freeze-drying chamber to return the air pressure to 1 atmosphere Return to room temperature at a natural heating rate, and take out the freeze-dried fabric.

Example 3:

A method for finishing fabrics with plant essential oil thermosensitive liposomes, comprising the steps of:

(1) Mix and stir tea tree essential oil, menthol and mugwort essential oil at a mass ratio of 2:1:2 at 2°C at a stirring speed of 60 rpm to obtain a natural aromatic antibacterial agent.

(2) Prepare phosphate buffer solution: sodium chloride 8g/L, potassium chloride 0.2g/L, disodium hydrogen phosphate 1.44g/L, dipotassium hydrogen phosphate 0.24g/L, adjust pH to 6.5 with hydrochloric acid, add The final concentration was 6% v/v Tween 80. Dissolve the natural fragrance antibacterial agent in the above-mentioned phosphate buffer solution, so that the concentration of the natural fragrance antibacterial agent is 20g/L.

(3) Egg yolk lecithin, distearoyl lecithin, distearoylphosphatidylglycerol sodium salt, dipalmitoylphosphatidylethanolamine, distearoylphosphatidylserine sodium salt and dimyristoylphosphatidic acid sodium salt The ratio of the amount of substances is 1:1:1:1:1:1. Take 100 mg of phospholipid mixture and 25 mg of cholesterol, dissolve in chloroform and pour into a round bottom flask, and rotate at room temperature until a uniform film is formed on the wall of the bottle.

(4) Preheat the phosphate buffer solution containing natural antibacterial agents to 55°C in an ultrasonic water bath at 55°C, and add it to a round-bottomed flask covered with a film, and oscillate ultrasonically for 15 minutes at a frequency of 400MHz and 200 rpm /min, the mass ratio of natural aromatic antibacterial agent (not including the quality of phosphate buffer solution) to film is 2:1, and a thermosensitive liposome suspension is prepared.

(5) Add chitosan as an adhesive to the heat-sensitive liposome suspension at a concentration of 20 g/L, stir at room temperature until completely dissolved, and obtain a heat-sensitive aromatic antibacterial finishing agent.

(6) Pad the fabric in the finishing solution with a bath ratio of 4:1, two dips and two rolls, the roll pressure is 0.2MPa, and the excess rate is 95%; finally, the fabric drops from room temperature at a rate of 1°C/min to -80°C, then turn on the vacuum pump for freeze-drying, the vacuum degree is less than 13Pa, maintain at -80°C for 24 hours, then raise the temperature to -55°C at a rate of 0.5°C/min, inject nitrogen into the freeze-drying chamber to return the air pressure to 1 atmosphere Return to room temperature at a natural heating rate, and take out the freeze-dried fabric.

Comparative example 1:

A method for finishing fabrics with plant essential oils, comprising the steps of:

(1) Mix tea tree essential oil, menthol and mugwort essential oil at a mass ratio of 2:1:2 at 2°C with a stirring speed of 60 rpm to obtain a natural aromatic antibacterial agent;

(2) Prepare phosphate buffered saline solution: sodium chloride 8g/L, potassium chloride 0.2g/L, disodium hydrogen phosphate 1.44g/L, dipotassium hydrogen phosphate 0.24g/L, adjust pH to 6.5 with hydrochloric acid, add The final concentration was 6% v/v Tween 80.

(3) Add 20g/L natural aromatic antibacterial agent and 20g/L chitosan to the phosphate buffer solution, stir at room temperature until completely dissolved, and obtain an aromatic antibacterial finishing agent.

(4) The fabric is dipped in the finishing solution at a bath ratio of 4:1, two dips and two rolls, the roll pressure is 0.2MPa, and the excess rate is 95%; finally, the fabric drops from room temperature at a rate of 1°C/min to -80°C, then turn on the vacuum pump for freeze-drying, the vacuum degree is less than 13Pa, maintain at -80°C for 24 hours, then raise the temperature to -55°C at a rate of 0.5°C/min, inject nitrogen into the freeze-drying chamber to return the air pressure to 1 atmosphere Return to room temperature at a natural heating rate, and take out the freeze-dried fabric.

Comparative example 2:

A method for finishing fabrics with plant essential oils, comprising the steps of:

(1) Mix tea tree essential oil, menthol and mugwort essential oil at a mass ratio of 2:1:2 at 2°C with a stirring speed of 60 rpm to obtain a natural aromatic antibacterial agent;

(2) Prepare phosphate buffered saline solution: sodium chloride 8g/L, potassium chloride 0.2g/L, disodium hydrogen phosphate 1.44g/L, dipotassium hydrogen phosphate 0.24g/L, adjust pH to 6.5 with hydrochloric acid, add The final concentration was 6% v/v Tween 80.

(3) Add 20g/L natural aromatic antibacterial agent and 20g/L chitosan to the phosphate buffer solution, stir at room temperature until completely dissolved, and obtain an aromatic antibacterial finishing agent.

(4) Pad the fabric in the finishing solution according to the bath ratio of 4:1, double dipping and second rolling, the roll pressure is 0.2MPa, and the excess rate is 95%; the fabric treated by the second dipping and second rolling is placed in an oven at 110°C Bake for 5 minutes, then wash with water and dry to set the shape.

Example 4:

Determination of essential oil and menthol content:

A certain amount of sample was weighed, extracted with ethanol, and then the content of essential oil was tested by absorbance at 265nm, and the content of menthol was determined by gas chromatography. Gas chromatography method: the chromatographic column is SE-5430m×0.32m×0.25μm; the carrier gas is nitrogen, the flow rate is 1ml/min; the temperature is programmed, 70°C is maintained for 1min, then the temperature is raised at 5°C/min, and finally 140°C is maintained for 1min ; The temperature of the injection port is 250°C, and the temperature of the detector is 250°C; the detector is a hydrogen ion flame detector; the split ratio is 50:1; the injection volume is 0.4 μl.

The results of determination of essential oil and menthol content are shown in Table 1.

Table 1

Essential oil content mg/g Menthol content mg/g Example 1 58 18 Example 2 95 12 Example 3 78 14 Comparative example 1 75 13 Comparative example 2 7 1

Embodiment 1, 2, 3 and comparative example 1 adopt freeze-drying post-finishing technology to finish the fabric, make most of the essential oil and menthol remain in the fabric, and comparative example 2 adopts high-temperature drying to finish the fabric, make the essential oil component and peppermint Alcohol evaporates quickly, leaving only traces of essential oils and menthol in the fabric.

Determination of aroma release: Put the sample in an environment of 25°C, 1 standard atmospheric pressure, and 50% humidity. Take out the sample every month, cut a 1cm×1cm square, extract with ethanol, and measure the menthol content. Menthol content is 100%.

The results of the menthol content of the five fabrics prepared in the above examples and comparative examples are shown in FIG. 1 .

Compared with the comparative examples, the heat-sensitive liposome technology used in Examples 1, 2, and 3 effectively reduces the volatilization rate of menthol and achieves the effect of long-term release of fragrance. The prepared fabric has mint fragrance, and as time goes by, the fragrance smell is gradually released and weakened, the fragrance can create a pleasant and comfortable environment, relieve and release pressure, and contribute to people's physical and mental health.

The mensuration of the heat-sensitive essential oil release characteristic of fabric: by the five kinds of fabrics that above-mentioned embodiment and comparative example make, get 12 groups of test tubes and place respectively in 32,33,34,35,36,37,38,39,40,41 ,42 A total of 12 groups of water baths with different temperatures were preheated for 15 minutes, each test tube was added with 0.1g of fabric, shaken, and after 72 hours, the test tubes were quickly taken out of each group of water baths and placed in liquid nitrogen to cool, samples were taken, and extracted with ethanol. The essential oil content was measured at 265nm respectively, and the release rate was calculated, as shown in Figure 2.

Examples 1, 2, and 3 use thermosensitive liposome technology to enable the fabric to release the essential oil component as the temperature changes, while the comparative example has no such effect.

Fabric washability test: by the five kinds of fabrics that above-mentioned embodiment and comparative example make, according to AATCC100-2004 and AATCC124-2006 standard test its anti-staphylococcus aureus, Escherichia coli, Candida albicans, trichophyton rubrum after washing bacteria, Trichophyton mentagrophytes, and Epidermophyton flocculus are shown in Table 2.

Table 2

The fabric finished by the present invention has significant inhibitory effects on Staphylococcus aureus, Escherichia coli, Candida albicans, Trichophyton rubrum, Trichophyton mentagrophytes, and Epidermophyton flocculus, and the antibacterial effect is released slowly. It maintains a high antibacterial rate after multiple washings. In the comparative example, after repeated washing, the inhibitory effect on various microorganisms was significantly reduced.

Claims (8)

1. a method for plant essential oil thermosensitive liposome finishing fabric, is characterized in that, comprises the steps: (1) Mix and stir tea tree essential oil, menthol and Artemisia argyi essential oil at low temperature to obtain a natural aromatic antibacterial agent; (2) the natural aromatic antibacterial agent that step (1) obtains is dissolved in the phosphate buffered saline solution; (3) Mix natural phospholipids, phosphatidylcholine, glycerophospholipids, phosphatidylethanolamine, phosphatidylserine and phosphatidic acid, add cholesterol, dissolve in chloroform and pour into a container, and evaporate to the container wall at room temperature to form a uniform layer film; (4) Add the phosphate buffered saline solution of the natural aromatic antibacterial agent obtained in step (2) in the ultrasonic water bath to the container covered with film obtained in step (3), and obtain the thermosensitive liposome suspension after ultrasonic oscillation; (5) adding the adhesive to the heat-sensitive liposome suspension obtained in step (4), and stirring at room temperature until completely dissolved to obtain a heat-sensitive aromatic antibacterial finishing agent; (6) padding the fabric in the finishing solution, two dipping and two rolling, and finally the fabric is finished and shaped by freeze-drying; In step (3), described natural phospholipid is selected from soybean lecithin or egg yolk lecithin; Described phosphatidylcholine is selected from 1-myristoyl-2-stearoyl lecithin, dipalmitoyl lecithin, Distearoyl lecithin or 1-palmitoyl-2-stearoyl lecithin; described glycerophospholipid is selected from dipalmitoylphosphatidylglycerol sodium salt or distearoylphosphatidylglycerol sodium salt; described phospholipid Acylethanolamine is selected from dimyristoylphosphatidylethanolamine, dipalmitoylphosphatidylethanolamine or distearoylphosphatidylethanolamine; described phosphatidylserine is selected from dipalmitoylphosphatidylserine sodium salt or distearoylphosphatidylethanolamine Serine sodium salt; the phosphatidic acid is selected from dimyristoyl phosphatidic acid sodium salt, dipalmitoyl phosphatidic acid sodium salt or dimyristoyl phosphatidic acid sodium salt; the natural phospholipids, phosphatidylcholine, glycerophospholipid , phosphatidylethanolamine, phosphatidylserine and phosphatidic acid are mixed in a ratio of 1:1:1:1:1:1; natural phospholipids, phosphatidylcholine, glycerophospholipids, phosphatidylethanolamine, phosphatidylserine and The mass ratio of phosphatidic acid mixture to cholesterol is 4-9:1. 2. the method for plant essential oil thermosensitive liposome finishing fabric according to claim 1, is characterized in that, in step (1), the mass ratio of described tea tree essential oil, menthol and Folium Artemisiae Argyi quintessence oil is 1～3.5:1 :1～3, the stirring temperature is 0～4°C, and the stirring speed is 30～120 rpm. 3. the method for finishing fabric with plant essential oil thermosensitive liposome according to claim 1, is characterized in that, in step (2), natural fragrance antibacterial agent adds phosphate buffered saline with the concentration of 15～30g/L. 4. the method for plant essential oil thermosensitive liposome finishing fabric according to claim 1, is characterized in that, in step (4), in 55 ℃ of supersonic water baths, the phosphate buffer solution preheating that will contain natural aromatic antiseptic to 55°C, and added to a container covered with a film, ultrasonically oscillating for 10 to 50 minutes to obtain a thermosensitive liposome suspension, the ultrasonic frequency is 400MHz, and the oscillation frequency is 200 rpm; It is 3:2~1. 5. the method for plant essential oil thermosensitive liposome finishing fabric according to claim 1, is characterized in that, in step (5), described tackiness agent is selected from starch, chitosan, sodium alginate, carboxymethyl Cellulose, dextrin or polyvinyl alcohol; the amount of the adhesive relative to the thermosensitive liposome suspension is 15-30 g/L. 6. the method for plant essential oil thermosensitive liposome finishing fabric according to claim 1, it is characterized in that, in step (6), fabric is 3～5:1 padding in finishing liquid by bath ratio, two Dipping and rolling, the pressure of the roll is 0.1-0.25MPa, and the excess rate is 80-100%. 7. the method for finishing fabric with vegetable essential oil thermosensitive liposome according to claim 1, is characterized in that, in step (6), described freeze-drying method program is: at first from room temperature with the rate of 1 ℃/minute Decrease to -80°C, then turn on the vacuum pump for freeze-drying, the vacuum degree is less than 13Pa, maintain at -80°C for 24 hours, then raise the temperature to -55°C at a rate of 0.5°C/min, inject nitrogen into the freeze-drying chamber to bring the pressure back to 1 Atmospheric pressure, return to room temperature at a natural heating rate, and take out the freeze-dried fabric. 8. The fabric prepared by the method for finishing fabric with vegetable essential oil thermosensitive liposome according to any one of claims 1 to 7.