CN103966086A - Internal and external hierarchically-adjusted and controlled light source system capable of adjusting and controlling light intensity and adjusting light color - Google Patents
Internal and external hierarchically-adjusted and controlled light source system capable of adjusting and controlling light intensity and adjusting light color Download PDFInfo
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Abstract
本发明涉及一种可调控光强可调光色的内外置分级调控光源系统,包含LED灯管组、反应罐体、反应罐上盖、光强控制器、光照传感器组、反应罐支架和透明玻璃管,所述的透明玻璃管固定在反应罐上盖正中间位置,所述的反应罐上盖上设有光照传感器引出口,所述的反应罐支架固定在反应罐体底部,所述的LED灯管组与外部光强控制器连接,所述的光照传感器安装在反应罐中透明管与反应罐外壁的中间位置,所述的光强控制器与光照传感器通过经由光照传感器引出口的数据线连接。本发明的优点在于,使用户对饵料微藻反应器的操作更加方便;光源设备适合不同直径的反应器和藻类培养的光谱需求;光强调控可提供可靠和精确的测量参数。
The invention relates to an internal and external graded control light source system with adjustable light intensity and adjustable light color, which comprises an LED lamp tube group, a reaction tank body, a reaction tank upper cover, a light intensity controller, an illumination sensor group, a reaction tank bracket and a transparent The glass tube, the transparent glass tube is fixed at the middle position of the upper cover of the reaction tank, the upper cover of the reaction tank is provided with an outlet for the light sensor, the bracket of the reaction tank is fixed at the bottom of the reaction tank, and the The LED lamp tube group is connected with an external light intensity controller, and the light sensor is installed in the middle of the transparent tube in the reaction tank and the outer wall of the reaction tank, and the light intensity controller and the light sensor pass the data from the outlet of the light sensor line connection. The invention has the advantages of making the operation of the bait microalgae reactor more convenient for users; the light source equipment is suitable for reactors with different diameters and the spectral requirements of algae cultivation; the light intensity control can provide reliable and accurate measurement parameters.
Description
【技术领域】【Technical field】
本发明涉及渔业养殖微环境监控领域,具体地说,是一种可调控光强可调光色的内外置分级调控光源系统。 The invention relates to the field of micro-environment monitoring for fish farming, in particular to an internal and external graded control light source system with adjustable light intensity and adjustable light color.
【背景技术】【Background technique】
关于渔业微藻养殖微环境监控领域的现有技术中,存在如下缺陷: In the existing technology in the field of microenvironment monitoring for fishery microalgae cultivation, there are the following defects:
1.一些饵料微藻光生物反应器只采用自然光作为系统的光源,当微藻生长到一定阶段,微藻细胞密度增加,微藻细胞自身的互相遮挡使得微藻光生物反应器中心区域的光照强度变得很弱,难以满足微藻快速生长的需要。 1. Some bait microalgae photobioreactors only use natural light as the light source of the system. When the microalgae grows to a certain stage, the density of microalgae cells increases, and the mutual occlusion of the microalgae cells themselves makes the light in the central area of the microalgae photobioreactor The strength becomes very weak, and it is difficult to meet the needs of the rapid growth of microalgae.
2.一些饵料微藻光生物反应器采用的是以自然光作为外部光源,辅以单根灯管作为内置光源的设计方式。反应罐内部安装部署单根灯管作为内部光源,尽管系统通过安装内置光源达到了增加光照强度的效果,但是当反应罐外部自然光线较弱时,仍难以达到饵料微藻生长的最佳光照强度。 2. Some bait microalgae photobioreactors use natural light as the external light source, supplemented by a single lamp tube as the built-in light source. A single lamp tube is installed inside the reaction tank as the internal light source. Although the system achieves the effect of increasing the light intensity by installing a built-in light source, it is still difficult to achieve the optimal light intensity for the growth of bait microalgae when the natural light outside the reaction tank is weak. .
3.一些饵料微藻光生物反应器采用的是以自然光作为外部光源,反应罐内部安装部署多根灯管作为内部光源的设计方式,这种光源设计方式提高了光照效果,但又会使得反应罐内部的结构太过复杂,影响罐内部培养液的循环流动,并且在夜间因无外部光源,光照效果效果不佳。 3. Some bait microalgae photobioreactors use natural light as the external light source, and multiple lamp tubes are installed and deployed inside the reaction tank as the internal light source. This light source design improves the lighting effect, but it will make the reaction The internal structure of the tank is too complicated, which affects the circulation of the culture solution inside the tank, and at night because there is no external light source, the lighting effect is not good.
4.大多数光生物反应器采用的是日光灯管,极易破碎,不仅安装检修非常困难,而且发热量大,不宜控制温度,能量转化效率低。 4. Most photobioreactors use fluorescent tubes, which are easily broken, not only difficult to install and maintain, but also generate a lot of heat, which is not suitable for temperature control and low energy conversion efficiency.
5.一些光生物反应器采用的是LED灯管,但灯管数量固定,光色固定,可提供的光照强度和光谱范围无法调节,只适宜培养固定种类的藻类,而大多数藻种培养以蓝光和白光相结合的效果较好。 5. Some photobioreactors use LED lamps, but the number of lamps is fixed, the light color is fixed, and the light intensity and spectral range that can be provided cannot be adjusted. It is only suitable for cultivating fixed types of algae, while most algae are cultivated A combination of blue light and white light works better.
现有的饵料微藻光生物反应器光源设计的可调节性不足,不能随着微藻细胞的不同培养阶段动态地提供最佳的光照强度,也不能随着微藻种类的变化提供最佳光谱的光照效果。因为对于不同种类的微藻在同等光强条件下,单色光源对微藻的生长效率的影响大小是不尽相同的;另外在微藻的生长过程中,反应罐内的透光度是非线性变化的,而现有的光源设计系统所提供的光照值固定光色固定,不能根据反应罐内微藻细胞浓度的变化动态调整光照强度和光谱范围,为反应桶内细胞提供足够的光照和适宜不同藻种光合反应的光谱,使得微藻培养的产率增加相对较低,光转化效率较低。 The light source design of the existing bait microalgae photobioreactor has insufficient adjustability, and cannot dynamically provide the best light intensity with the different culture stages of microalgae cells, nor can it provide the best spectrum with the change of microalgae species lighting effects. Because for different types of microalgae under the same light intensity conditions, the influence of monochromatic light source on the growth efficiency of microalgae is not the same; in addition, during the growth of microalgae, the light transmittance in the reaction tank is nonlinear However, the light value provided by the existing light source design system is fixed and the light color is fixed. It cannot dynamically adjust the light intensity and spectral range according to the change of the concentration of microalgae cells in the reaction tank, so as to provide enough light and suitable for the cells in the reaction tank. The spectrum of photosynthetic reactions of different algae species makes the yield increase of microalgae culture relatively low, and the photoconversion efficiency is low.
中国专利文献CN101724547A公开了一种一种用LED可调光进行微藻培养实验的方法和装置。但其不能根据反应罐内微藻细胞浓度的变化动态调整光照强度和光谱范围。目前,关于一种可自动调节光色、光强,并针对反映罐内的微藻细胞浓度的变化动态调整光照强度和光谱范围的设备或方法还未见相关报道。 Chinese patent document CN101724547A discloses a method and device for microalgae cultivation experiments using LED adjustable light. However, it cannot dynamically adjust the light intensity and spectral range according to the change of the concentration of microalgae cells in the reaction tank. At present, there is no relevant report on a device or method that can automatically adjust light color and light intensity, and dynamically adjust light intensity and spectral range to reflect changes in the concentration of microalgae cells in the tank.
【发明内容】【Content of invention】
本发明的目的是针对现有技术中的不足,提供可调控光强可调光色的内外置分级调控光源系统。 The object of the present invention is to address the deficiencies in the prior art and provide an internal and external graded controllable light source system with adjustable light intensity and adjustable light color.
为实现上述目的,本发明采取的技术方案是: For realizing above-mentioned object, the technical scheme that the present invention takes is:
一种可调控光强可调光色的内外置分级调控光源系统,包含LED灯管组、反应罐体、反应罐上盖、反应罐支架和透明玻璃管,所述的反应罐上盖盖在反应罐体上,所述的透明玻璃管固定在反应罐上盖正中间位置,所述的反应罐支架固定在反应罐体底部,所述的LED灯管安装在反应罐的支架上和反应罐内部的透明玻璃管中,还包括光强控制器和光照传感器组,所述的光照传感器安装在反应罐中透明管与反应罐外壁的中间位置,所述的光强控制器与光照传感器通过经由反应罐上盖的光照传感器引出口的数据线连接,所述的光强控制器与每只灯管连接。 An internal and external hierarchical control light source system with adjustable light intensity and adjustable light color, including LED lamp tube group, reaction tank body, reaction tank top cover, reaction tank support and transparent glass tube. On the reaction tank, the transparent glass tube is fixed in the middle of the upper cover of the reaction tank, the support of the reaction tank is fixed at the bottom of the reaction tank, and the LED light tube is installed on the support of the reaction tank and the reaction tank The internal transparent glass tube also includes a light intensity controller and a light sensor group. The light sensor is installed in the middle of the transparent tube in the reaction tank and the outer wall of the reaction tank. The light intensity controller and the light sensor pass through The data line of the light sensor outlet on the upper cover of the reaction tank is connected, and the light intensity controller is connected with each lamp tube.
所述的光照传感器组为同一位置背靠背安装的2个光照度传感器。 The light sensor group is two light intensity sensors installed back to back at the same position.
所述的反应罐支架为可伸缩结构。 The reaction tank support is a telescopic structure.
所述的LED灯管共5只,其中一只安装在反应罐内部的透明玻璃管中,其余四只安装在反应罐支架的上,固定在反应罐的前后左右四个方向。 There are 5 LED lamp tubes in total, one of which is installed in the transparent glass tube inside the reaction tank, and the other four are installed on the support of the reaction tank, and are fixed in the front, rear, left, and right directions of the reaction tank.
所述的光照传感器组采用多传感器数据融合算法。 The illumination sensor group adopts a multi-sensor data fusion algorithm.
所述的光强控制器采用模糊逻辑光强分级调控方法。 The light intensity controller adopts a fuzzy logic light intensity graded control method.
本发明的优点在于: The advantages of the present invention are:
1. 采用内外置可分级调控光强的光源设备的设计方法,内置灯管数为1个,减少了桶内灯管的数量,优化了反应桶的结构,使得用户对饵料微藻反应器的操作更加方便,对反应桶的清洗也更加方便。光源采用LED灯管,灯管距反应罐位置可调,LED灯管光色可调,使得光源设备适合不同直径的反应器和不同藻类培养的光谱需求。 1. The design method of light source equipment with internal and external adjustable light intensity can be adopted. The number of built-in lamps is 1, which reduces the number of lamps in the barrel and optimizes the structure of the reaction barrel. The operation is more convenient, and the cleaning of the reaction barrel is also more convenient. The light source adopts LED lamp tube, the distance between the lamp tube and the reaction tank is adjustable, and the light color of the LED lamp tube is adjustable, so that the light source equipment is suitable for reactors with different diameters and the spectral requirements of different algae cultivation.
2. 采用多传感器测量和传感器数据融合方法测量和计算反应罐内的光照强度值,为光强调控提供可靠和精确的测量参数。 2. Using multi-sensor measurement and sensor data fusion methods to measure and calculate the light intensity value in the reaction tank, to provide reliable and accurate measurement parameters for light intensity control.
3. 采用模糊逻辑光强分级调控方法,可以根据反应罐内微藻的生长状况和微藻培养过程需光量,动态调控各组内外置光源的亮灭,提高了光照控制的精准度,使得微藻的生长始终能获得更好的光照条件,进而使得微藻的培养产率更高,对微藻养殖和精细渔业的发展有着很大的推动。 3. Using fuzzy logic light intensity graded control method, it can dynamically control the on and off of the internal and external light sources in each group according to the growth status of the microalgae in the reaction tank and the amount of light required during the microalgae cultivation process, which improves the accuracy of light control and makes the microalgae The growth of algae can always obtain better light conditions, which in turn makes the culture yield of microalgae higher, which greatly promotes the development of microalgae farming and fine fishery.
【附图说明】【Description of drawings】
附图1是本发明的结构示意图。 Accompanying drawing 1 is a structural representation of the present invention.
附图2是光强控制器工作流程图。 Accompanying drawing 2 is the working flow chart of light intensity controller.
附图3是模糊逻辑光强分级调控方法框图。 Accompanying drawing 3 is a block diagram of fuzzy logic light intensity graded control method.
附图4是采用带有内外置分级调控光源的光生物反应器中培养饵料微藻的工艺过程。 Accompanying drawing 4 is the process of cultivating bait microalgae in a photobioreactor with internal and external graded control light sources.
表格一是以亚心形扁藻为实施例的实验数据表格。 Table 1 is the experimental data table of Hymenopsis subcordiformis as an example.
【具体实施方式】【Detailed ways】
下面结合附图对本发明提供的具体实施方式作详细说明。 The specific embodiments provided by the present invention will be described in detail below in conjunction with the accompanying drawings.
附图中涉及的附图标记和组成部分如下所示: The reference signs and components involved in the accompanying drawings are as follows:
1.光强控制器 2.光照传感器组 1. Light intensity controller 2. Light sensor group
3.LED灯管组 4.反应罐支架 3. LED lamp group 4. Reaction tank support
5.反应罐体 6.透明玻璃管 5. Reactor body 6. Transparent glass tube
7.反应罐上盖 8.光照传感器引出口 7. Reaction tank cover 8. Light sensor outlet
请参见图1,图1所示为本发明一种可调控光强可调光色的内外置分级调控光源系统的结构示意图。所述的内外置光源系统包含反应罐体5、反应罐上盖7、反应罐支架4、LED灯管组3、光强控制器1、光照传感器组2和透明玻璃管6。所述的反应罐上盖7盖在反应罐体5上,所述的透明玻璃管6固定在反应罐上盖7正中间位置。所述的反应罐上盖7上设有光照传感器引出口8。所述的反应罐支架4固定在反应罐体5底部,反应罐支架8为可伸缩结构,可调节底部伸缩长度。所述的LED灯管组3共5只LED灯管,其中一只LED灯管安装在与反应罐上盖7相连的透明玻璃管6中,其他LED灯管安装在反应罐的支架4上,固定在反应罐前后左右四个方向,每只灯管均与外部光强控制器1连接。所述的光照传感器组2为光照测量设备,安装在反应罐中透明管6与反应罐外壁的中间位置。所述的光强控制器1与光照传感器组2通过经由光照传感器引出口8的数据线连接。 Please refer to FIG. 1 . FIG. 1 is a schematic structural diagram of an internal and external hierarchically adjustable light source system with adjustable light intensity and adjustable light color according to the present invention. The internal and external light source system includes a reaction tank body 5 , a reaction tank cover 7 , a reaction tank support 4 , an LED lamp tube group 3 , a light intensity controller 1 , an illumination sensor group 2 and a transparent glass tube 6 . The reaction tank upper cover 7 is covered on the reaction tank body 5, and the transparent glass tube 6 is fixed at the middle position of the reaction tank upper cover 7 . The upper cover 7 of the reaction tank is provided with a light sensor outlet 8 . The reaction tank support 4 is fixed on the bottom of the reaction tank body 5, and the reaction tank support 8 is a telescopic structure, which can adjust the telescopic length of the bottom. The LED lamp tube group 3 has 5 LED lamp tubes in total, one of which is installed in the transparent glass tube 6 connected to the upper cover 7 of the reaction tank, and the other LED lamp tubes are installed on the bracket 4 of the reaction tank, It is fixed in the front, back, left, and right directions of the reaction tank, and each lamp tube is connected with an external light intensity controller 1 . The light sensor group 2 is light measuring equipment, which is installed in the intermediate position between the transparent tube 6 and the outer wall of the reaction tank in the reaction tank. The light intensity controller 1 is connected with the light sensor group 2 through the data line through the light sensor outlet 8 .
需要说明的是,所述的LED灯为线状LED灯,功率为25w,可为反应罐表面提供4000~5000lux光照强度,LED灯的光色可随培养的藻种做相应调整。光照传感器测量到的数据由数据线经光照测量设备引出口传输到光强控制器上,光强控制器对光源设备进行光强调控。外部的四个灯管安装在罐底部支架的伸出部分,该部分的长度可以调整。因此,此套光源设备可以安置在不同内径的反应罐上,为多种光反应器提供光源。 It should be noted that the LED lamp is a linear LED lamp with a power of 25w, which can provide 4000-5000 lux light intensity on the surface of the reaction tank, and the light color of the LED lamp can be adjusted accordingly with the cultured algae. The data measured by the light sensor is transmitted to the light intensity controller through the data line through the outlet of the light measurement device, and the light intensity controller controls the light intensity of the light source device. The four light tubes on the outside are installed on the protruding part of the bottom bracket of the tank, and the length of this part can be adjusted. Therefore, this set of light source equipment can be placed on reaction tanks with different inner diameters to provide light sources for various photoreactors.
光源设备中LED灯的选择,不同LED光源对微藻的生长效率的影响是不同的,对于大多数藻种来说,同等光强条件下,单色光源对微藻的生长效率的影响大小顺序是:蓝光LED>红光LED>绿光LED。不同光源组合对亚心形扁藻的生长速率的影响: 红光+蓝光LED>绿光+蓝光LED>红光+蓝光+绿光LED>红光+绿光LED; 不同光源组合对纤细角毛藻的生长速率的影响:红光+蓝光+绿光LED>绿光+蓝光LED>红光+蓝光LED>红光+绿光LED。所以根据不同藻种可采用不同光色的LED灯,改变光谱范围,可为不同藻种提供更最佳光源。 The choice of LED lights in light source equipment, different LED light sources have different effects on the growth efficiency of microalgae. For most algae species, under the same light intensity, the order of the effect of monochromatic light source on the growth efficiency of microalgae Yes: Blue LED>Red LED>Green LED. Effects of different light source combinations on the growth rate of P. subcordiformis: red light + blue LED > green + blue LED > red + blue + green LED > red + green LED; The influence of the growth rate of algae: red light + blue light + green light LED > green light + blue light LED > red light + blue light LED > red light + green light LED. Therefore, LED lights with different light colors can be used according to different algae species, and the spectral range can be changed to provide more optimal light sources for different algae species.
这种结构布局设计方式既可为多种光反应器提供最佳光源,又减少了罐内灯管的数量,优化了反应罐的结构。 This structural layout design method can not only provide optimal light sources for various photoreactors, but also reduce the number of lamp tubes in the tank and optimize the structure of the reaction tank.
请参见图2,图2所示为光强控制器工作流程图。所述的光强控制器由模糊逻辑内外置分级调控LED灯管组、光照传感器组、电源模块、微处理器、隔离保护装置、模糊逻辑光强分级调控系统、多传感器数据采集系统、光强调控模糊算法、多传感器数据融合算法组成。所述的多传感器数据采集系统与微处理器相连,将光照传感器组(同一位置背靠背安装一组光照度传感器)检测到的信号输送至微处理器,微处理器运用光强调控模糊算法、多传感器数据融合算法将数据进行计算后,将运算结果输送至模糊逻辑光强分级调控系统中,控制系统对模糊逻辑内外置分级调控LED灯光组进行不同工作状态的调控。 Please refer to Fig. 2, Fig. 2 shows the working flow diagram of the light intensity controller. The light intensity controller is composed of fuzzy logic internal and external graded control LED lamp tube group, light sensor group, power supply module, microprocessor, isolation protection device, fuzzy logic light intensity graded control system, multi-sensor data acquisition system, light intensity Control fuzzy algorithm, multi-sensor data fusion algorithm. The multi-sensor data acquisition system is connected to the microprocessor, and the signal detected by the illumination sensor group (a group of illumination sensors installed back to back at the same position) is sent to the microprocessor, and the microprocessor uses the light intensity control fuzzy algorithm, multi-sensor After the data fusion algorithm calculates the data, the calculation result is sent to the fuzzy logic light intensity classification control system, and the control system controls the different working states of the fuzzy logic internal and external hierarchical control LED lighting groups.
需要说明的是,本设计中将光照设备的工作状态分为如下四种,使光强可进行分级调控,所有灯管都关闭为工作状态0,单独开启内置灯管为工作状态1,再开启外置光源的任意对角线上的两根灯管为工作状态2,所有灯管都开启为工作状态3。 It should be noted that in this design, the working status of the lighting equipment is divided into the following four types, so that the light intensity can be adjusted in stages. All the lamps are turned off as the working status 0, and the built-in lamps are turned on separately as the working status 1, and then turned on Two light tubes on any diagonal line of the external light source are in working state 2, and all light tubes are turned on in working state 3.
(1)光强测量的多传感器数据融合算法: (1) Multi-sensor data fusion algorithm for light intensity measurement:
光反应器的传感器是安装在反应器的中心和反应器的外壁之间的中间位置,因为传感器上的光感应器件的感应角度无法实现对反应器内外两侧的光照度同时感应,所以在同一位置背靠背安装一组光照度传感器。 The sensor of the photoreactor is installed in the middle between the center of the reactor and the outer wall of the reactor, because the sensing angle of the photosensitive device on the sensor cannot realize the simultaneous sensing of the illuminance on both sides of the reactor, so in the same position Install a set of illuminance sensors back to back.
利用传感器采集数据时,要把传感器的内部噪声与环境干扰进行综合分析。纵向分析:针对一个传感器多次采样结果的分析,以单个传感器为研究对象,测量方差是传感器内部噪声与环境干扰的一种综合属性,这一属性始终存在于测量的全过程中,一次偶然的干扰可能会对最后得到的结果产生较大误差。横向分析: 横向分析中利用了多传感器在某一采样时刻的测量信息。 When using sensors to collect data, it is necessary to conduct a comprehensive analysis of the internal noise and environmental interference of the sensor. Longitudinal analysis: for the analysis of multiple sampling results of a sensor, with a single sensor as the research object, the measurement variance is a comprehensive attribute of the internal noise and environmental interference of the sensor, which always exists in the whole process of measurement, and an accidental Interference may cause large errors in the final results. Horizontal analysis: In horizontal analysis, the measurement information of multiple sensors at a certain sampling time is used.
采用多个传感器进行采样时,由于传感器朝向的不同,在计算该位置光照度的时候,每个传感器的测量结果所占的权重也是不同的。一组分别朝内外两侧的两个光照度传感器所测量的反应器内培养液的体积比为1:3,所以对内外侧方向传感器采集的数据的权重设定为0.25和0.75。 When multiple sensors are used for sampling, due to the different orientations of the sensors, when calculating the illuminance of the location, the weight of the measurement results of each sensor is also different. The volume ratio of the culture solution in the reactor measured by a group of two illuminance sensors facing the inner and outer sides is 1:3, so the weights of the data collected by the inner and outer direction sensors are set to 0.25 and 0.75.
多传感器融合算法如下:设 表示第i个传感器短时间内第n次采样的结果,其中最大值为,最小的值为,则连续n次采样时各传感器测量算术平均值为: The multi-sensor fusion algorithm is as follows: Indicates the result of the nth sampling of the i-th sensor within a short period of time, where the maximum value is , the minimum value is , then each sensor measures the arithmetic mean value during n consecutive samplings for:
则安装在同一位置的背靠背a组传感器测得的光照度为: Then the illuminance measured by the back-to-back group A sensors installed at the same position is:
式中表示a位置朝向外侧的传感器测量算术平均值,表示a位置朝向内侧的传感器测量算术平均值。 In the formula Indicates the arithmetic mean of sensor measurements with a position facing outwards, Indicates the arithmetic mean of sensor measurements at a position towards the inside.
(2)模糊逻辑光强分级调控方法 (2) Fuzzy logic light intensity classification control method
请参见图3,图3所示为模糊逻辑光强分级调控方法框图。 Please refer to FIG. 3 , which is a block diagram of the fuzzy logic light intensity level control method.
模糊逻辑光强分级调控方法及策略如下:将整个光照系统的光源设备中的所有灯管开关分为四种工作状态,在反应罐中心与侧壁的中间位置背靠背放置两个光照传感器,传感器采集的光照强度信号,经过D/A转换电路处理之后,经上述多传感器融合算法得到罐内光照度值,同时计算出藻细胞浓度,作为模糊推理控制器的输入变量,接着再将此输入量作标准化处理,将其变化范围影射到响应论域中,通过隶属函数建立清晰量与模糊量之间的对应关系,确定输入值对应于语言变量的语言值作为模糊控制推理的输入。模糊控制推理以规则库和输入变量为依据,经模糊推理得到分级光源设备的模糊结果,通过控制光源设备的工作状态实现对光照强度的分级调控。规则库是根据饵料微藻培养过程的光需量模型和反应罐内细胞密度变化模型得到。 The fuzzy logic light intensity classification control method and strategy are as follows: divide all the lamp switches in the light source equipment of the entire lighting system into four working states, place two light sensors back to back at the middle position between the center of the reaction tank and the side wall, and the sensors collect After being processed by the D/A conversion circuit, the illuminance value in the tank is obtained by the above-mentioned multi-sensor fusion algorithm, and the algae cell concentration is calculated at the same time, which is used as the input variable of the fuzzy inference controller, and then this input is standardized Processing, mapping its range of change to the response domain, establishing the correspondence between clear and fuzzy quantities through membership functions, and determining the linguistic values corresponding to the input values of linguistic variables as the input of fuzzy control reasoning. The fuzzy control reasoning is based on the rule base and input variables, and the fuzzy results of the graded light source equipment are obtained through fuzzy reasoning, and the graded regulation of the light intensity is realized by controlling the working state of the light source device. The rule base is obtained according to the light demand model of the bait microalgae cultivation process and the cell density change model in the reaction tank.
微藻培养初期反应罐内的微藻浓度较低,外部的自然光可以满足微藻快速培养的需要,光照设备处于工作状态0(即所有灯管都关闭);随着微藻浓度的不断增加,靠近反应罐的中心位置的光照条件变差,光强调控系统将采集到的光照数据经模糊逻辑分析之后得到的模糊结果,与最适宜微藻生长的光照范围下限值(不同藻种的值是不同的)相比较,模糊结果一旦超出下限值,光照设备将进入工作状态1(即仅开启内置灯管),即最弱光照度LED灯管组,开启中心位置的一个LED灯光。模糊逻辑光强分级调控会根据藻种规则库设定LED灯的光色,使得反应罐内的光照条件处在最适宜生长的光谱范围,同时不断根据微藻浓度的变化,调节线状LED灯的光照强度。采取同样的调控策略控制何时进入工作状态2(开启内置灯管的同时开启外置光源的任意对角线上的两根灯管)和工作状态3(开启全部灯管)。 At the initial stage of microalgae cultivation, the concentration of microalgae in the reaction tank is low, and the external natural light can meet the needs of rapid cultivation of microalgae, and the lighting equipment is in the working state of 0 (that is, all lamps are turned off); The light conditions near the center of the reaction tank become worse. The light intensity control system analyzes the collected light data through fuzzy logic to obtain the fuzzy results and the lower limit value of the light range that is most suitable for the growth of microalgae. (of different algal species Values are different), once the fuzzy result exceeds the lower limit, the lighting device will enter working state 1 (that is, only the built-in lamp is turned on), that is, the LED lamp group with the weakest illuminance, and an LED light at the center will be turned on. The fuzzy logic light intensity classification control will set the light color of the LED light according to the algae rule library, so that the light conditions in the reaction tank are in the most suitable spectral range for growth, and at the same time continuously adjust the linear LED light according to the change of the concentration of microalgae light intensity. Adopt the same control strategy to control when to enter working state 2 (turn on the built-in light tube and turn on two light tubes on any diagonal of the external light source at the same time) and work state 3 (turn on all the light tubes).
相对于传统的简单控制方式,这种调控方式对光照条件的控制更加精确,可随着饵料微藻的生长需要、藻细胞的生长进程和外部自然光的变化动态调控光强。 Compared with the traditional simple control method, this regulation method can control the light conditions more accurately, and can dynamically adjust the light intensity according to the growth needs of bait microalgae, the growth process of algae cells and the changes of external natural light.
请参见图4,图4所示为采用带有内外置分级调控光源的光生物反应器中培养饵料微藻的工艺过程。饵料微藻培养首先需要对反应罐进行清洗,清除之前培养残留的微藻,防止残留物对本次培养的污染;然后根据培养藻种的需要将LED灯的光强和色度调节到最适合微藻生长的范围;准备工作做好之后,加入微藻藻种,设置控制器为自动调控状态,光反应器就进入了自动培养阶段,调控系统将实时采集到的反应罐内培养液光照度和细胞浓度值,根据多传感器测量和数据融合的模糊逻辑光强分级调控方法分级调控LED灯管组的工作状态,使得微藻的整个培养过程始终处于良好的光照条件下;同时控制系统还根据采集到的藻细胞浓度值来判断是否达到了最适合收获的浓度K,一旦达到目标浓度值就收获微藻,进入下次培养阶段,否则继续进行本次培养。 Please refer to Figure 4, which shows the process of cultivating microalgae as food in a photobioreactor with internal and external graded light sources. For bait microalgae cultivation, the reaction tank first needs to be cleaned to remove the residual microalgae from the previous cultivation to prevent the residue from polluting this cultivation; then adjust the light intensity and chromaticity of the LED lamp to the most suitable The range of microalgae growth; after the preparations are done, add microalgae species, set the controller to the automatic control state, and the photoreactor will enter the automatic cultivation stage. The control system will collect the real-time The cell concentration value, according to the multi-sensor measurement and data fusion fuzzy logic light intensity graded regulation method, grades and regulates the working status of the LED lamp group, so that the whole cultivation process of microalgae is always under good light conditions; at the same time, the control system also according to the collected The obtained algae cell concentration value is used to judge whether the concentration K that is most suitable for harvesting has been reached. Once the target concentration value is reached, the microalgae will be harvested and enter the next cultivation stage, otherwise continue this cultivation.
实施例1 Example 1
藻种:亚心形扁藻 Algae species: subcardioid flat algae
最佳光强范围:5000-1000lux Best light intensity range: 5000-1000lux
实验实时采集数据见表一: The real-time data collected in the experiment is shown in Table 1:
由上述表格可以看出,采用多传感器测量和数据融合的模糊逻辑光强调控方法可以实时监测并调控光强,使微藻时刻处于最佳光照强度下,极大的提高了培养效率。 It can be seen from the above table that the fuzzy logic light intensity control method using multi-sensor measurement and data fusion can monitor and adjust the light intensity in real time, so that the microalgae are always under the optimal light intensity, which greatly improves the cultivation efficiency.
以上所述仅是本发明的优选实施方式,应当指出,对于本技术领域的普通技术人员,在不脱离本发明方法的前提下,还可以做出若干改进和补充,这些改进和补充也应视为本发明的保护范围。 The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the method of the present invention, some improvements and supplements can also be made, and these improvements and supplements should also be considered Be the protection scope of the present invention.
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