CN103960031B - A kind of method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing - Google Patents
A kind of method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing Download PDFInfo
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Abstract
The invention discloses a kind of method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing. By adopting raise seedling in greenhouse substrate culture to be bred as seedling, the ridging of decomposing agent fertilizer is used, disinfection by ultraviolet light processing is carried out in raise seedling in greenhouse reply agate coffee seed and canopy chamber, nutritive soil preparation and seedbed with soil and seedling medium by weight the ratio of 5:1 fully mix, add 1% plant ash, raw microbacterium in employing beet (<i>Microbacterium? maritypicum</i>) as Antagonistic Fungi, adding root matrix soil to fill with root mode Antagonistic Fungi suspension, field planting cultivation applies high-quality decomposed manure according to 2000-2500 kg/acre, ploughing depth 15~20cm according to every mu; Flat planting bedding. The agate coffee cultivation cycle has shortened 2 months, and in the success of 3500 meters to 4500 meters Pamir Mountain Area plantation agate coffees of height above sea level, survival rate more than 98%, is with a wide range of applications first.
Description
Technical field
The present invention relates to the technical field of the planting of medicinal materials plantation. Specifically, the present invention relates to a kind of agate coffee and adapt to high seaLift the technical field of the field production plantation under latitude condition.
Background technology
Agate coffee (LepidiummeyeniiWalp.) claim again agate card, Ma Ka, and English is commonly called as MACA, for Cruciferae is walked alone2 years raw herbaceous plant of Lepidium. Medicinal part is underground radish shape or turbination meat rhizome, is about 10 ~ 14cm, diameter 3 ~5cm, can be divided into multiple types such as white, black, red, yellow according to color difference. Leaf green, is winglike compound leaf, and base is born in rhizome, crawlsCrawl is in ground, and leaf has 12~20, and bar shaped, is about 10~20cm, and wide 1~2cm has smooth meat axle, present situation drastic crack,Blade is rosette to be arranged, and new leaf is longer renewal the from rose center constantly. The general First Year of agate coffee isVegetative growth phase, Second Year is is just blossomed and had seeds, and in 3 ~ June of florescence, is raceme. Raceme top is raw, spends little and many, normalHave six stamens, generally only have two complete, only a few have three complete. Agate coffee is mainly self-pollination, the florescence 1~2Individual month, great majority were spent and can be formed fruit, and fruit is short horn shape, and long 4~5cm, splits into bicrescentic tubule, each fruitThere is a seed real the inside. 5 week of fruit can be ripe, and division discharges seed. Seed ovalize, long 2~2.5cm,Micro-cervinus. Agate coffee is octoploid plant, 64 chromosomes.
Agate coffee is not only nutritious food, is also the medicinal herbs with multiple magical effect, according to modern scientific research, and agateCoffee has multiple nutritional components, and as protein, amino acid, polysaccharide, multivitamin etc., in addition, agate coffee also contains and has special meritEffect active skull cap components agate coffee alkene, agate coffee acid amides, mustard oil, alkaloid, mineral trace element, have improve immunity,Pharmacological effects such as improving sexual function, regulate endocrine, antifatigue, be anti-oxidant, antitumor, can be used for improving and treatment clinicallySexual function, the climacteric metancholia of improving, treatment anaemia, enhancing muscle power, reducing blood lipid etc.
Due to the abundant nutritional labeling of agate coffee and special function effect, make it be subject to global attracting attention. Open at agate coffeeThe field of sending out, utilizes the character of agate coffee " herbal cuisine is dual-purpose " to develop various health foods and nutritious supplementary pharmaceutical more on market,There are in the world market prospects well.
The achievement in research that Peru biologist GloriaChacon in 1961 announces agate coffee (Maca) first obtains industryGreatly pay close attention to; Within 1992, FAO's Rome declaration is using agate coffee (Maca) as a kind of rare nutritious supplementary pharmaceutical to generationBoundary is recommended. Within 1992, International Plant Genetic Resources Institute classifies agate coffee (Maca) as rare plant. Within 1998, America plant is treatedMethod research institute publishes agate coffee (Maca) monograph " the medicinal and nutrient plant of agate coffee (Maca) Peru; International mountain-climbing tissue connection in 2000Alliance (UIAA) classifies agate coffee (Maca) as appointed product. Calendar year 2001 FDA Food and Drug Administration (FDA) has passed through agate coffee(Maca) health medicine enters the demonstration of the U.S.. Calendar year 2001 USPO's approval Section 1 is extracted effectively from agate coffee (Maca)The patented technology of composition. Calendar year 2001 the U.S. two kinds of novel substance called after agate coffee alkene and the agate coffee that will in agate coffee (Maca), findAcid amides. Calendar year 2001 American Space General Administration (NASA) is the space food using agate coffee (Maca) as astronaut first. Calendar year 2001 roadMagical effect of society's report agate coffee (Maca) thoroughly. Calendar year 2001 Chinese Central Television " 30 points of news " special report agate coffee (Maca)Newest research results. Calendar year 2001 China excitant and sport nutrition testing research center confirm that agate coffee (Maca) Bu Han is international difficult to understandCommittee can forbidding composition. Calendar year 2001 member of Chinese Academy of Engineering Xiao Peigen " health food that MACA-attractes attention in a whole world " literary composition is delivered.2002 agate coffee (Maca) become the world cup sportsman of Korean and Japanese and specify nutriment. Ministry of Health of China official approval agate coffee in 2002(Maca) enter China.
Calendar year 2001 MACA is just by the certification (FDA Food and Drug Administration) of U.S. FDA, the product of checking by FDAOn safety and effect problem, obtain approving, become the widely known health products in the whole world, on the medical science stage of the Bing worldPlay the part of more and more important role. Within 2011, health ministry is formally approved as new resource food by pueraria root powder. May 18 in 2011Day, in new resource food catalogue new resource food below, is listed agate card powder by the Ministry of Public Health---in newly development of China, new discovery, newIntroduce without food custom, meet food basic demand, the article nontoxic to human body claim new resource food.
At present, along with agate coffee is promoted in the plantation in Yunnan, agate coffee has increasing attraction in hill farmer. ButTo be that so far there are no relevant is reported in for 3500 meters of high and cold high height above sea level in the applicable Pamir Mountain Area to 4500 meter district agate coffee cultigensThe method of planting.
Summary of the invention
The present invention is directed to existing having no for the field production plantation agate coffee under the high height above sea level latitude condition of the Pamir Mountain AreaPresent situation, the present invention aims to provide a kind of technology of the method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growingScheme, creativeness provides a kind of method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing, large by adoptingCanopy is unified seedling medium and is cultivated seedling, and decomposing agent fertilizer ridging is used, and adopts Antagonistic Fungi to ensure the battalion in agate coffee seedling growth periodGood effect is all played in the demand of supporting and prevention and elimination of disease and pests, has practicality and using value widely.
The main technical schemes that the present invention adopts:
The invention provides a kind of method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing, by adoptingBooth is unified seedling medium and is cultivated seedling, and decomposing agent fertilizer ridging is used, and adopts Antagonistic Fungi to ensure in agate coffee seedling growth periodNutritional need and prevention and elimination of disease and pests, overcome the existing technical bottleneck existing for high height above sea level field planting agate coffee that has no,All play good effect for the agate coffee plantation of cultivating high viability, high yield, high-quality, there is practicality widelyAnd using value.
Further, the invention provides a kind of method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing, %According to weight percent meter, concrete growing and cultivation method is as follows.
(1) agate coffee seed pretreatment: agate coffee seed needs prior to seeding through K cryogenic treatment is preserved at-1 DEG C to-5 DEG C7-10 days with 600 times of liquid of approximately 45% Bravo and 800 times of liquid seed dressings of 55% carbendazol wettable powder, dries standby before growing seedlingsWith; Before sowing, adopt presoaking and germinating processing, seed pulled out and shakeout again with the cold water 16h-24h that at room temperature soaks seed, frequently stir,Soaking seed can be used for growing seedlings.
(2) adopt booth substrate seedling: before sowing, need raise seedling in greenhouse, select raise seedling in greenhouse facility, the reply agate of growing seedlingsDisinfection by ultraviolet light processing is carried out in coffee seed and canopy chamber, creates the environmental condition that is applicable to agate coffee seedling development; Nutritive soil preparationAnd seedbed with soil and seedling medium by weight the ratio of 5:1 fully mix, add 1% plant ash, the soil choosing of ground, seedbedSelect height above sea level 2700m to 3200m, select the leeward plot that faces south, select fertile loose neutral sandy loam, require loose, fertilizer conservation, guarantorWater, by the Nutrition Soil uniform spreading preparing, on seed bed, thickness 10cm-15cm waits to broadcast; Agate coffee is cultivated employing cave, seedbed dish and is educatedSeedling, agate coffee application rate is assessed according to 20~30 grams every mu; Cave dish is fitly emitted on to ground smooth in booth, then waters footWater, canopy chamber will ensure sufficient illumination, annual mid or late Febuary seeding and seedling raising, seedling age can be transplanted after 30~50 days in field planting, oneAs after 7 days agate coffee true leaf grow gradually, after 14 days, true leaf grows to 2~3cm, after 30~50 days, to grow to 6~8cm high for agate coffee true leafTransplant; Adopt during this time the interior raw microbacterium of beet (Microbacteriummaritypicum) as Antagonistic Fungi, to fill with rootMode Antagonistic Fungi suspension adds, and root irrigation adopts according to 1% Antagonistic Fungi sprays root soil, and pouring Antagonistic Fungi viable count is notLower than 108The bacteria suspension of cfu/mL.
(3) seedling medium: by wheat, corn stalk powder being broken into the section shape of 1-10mm, add water to water content 60-80%,Then by volume every cubic metre add urea 2-3kg, sheep excrement and cow dung are according to weight ratio 1:1 composition farm manure, farm manure is pressedEvery cubic metre of volume ratio adds 5-10kg, quick lime by volume every cubic metre add 1-2kg, compoiste fermented microbial inoculum is by volumeEvery cubic metre adds 500-1000g, and mixes, and at 15 DEG C of-40 DEG C of lower seal heap fermentations of air themperature, sealing is piled up and sent outFerment is to carry out under plastic covering film or straw screen or mat, fermentation start turning in latter every 10 days once, when turning moisturizing to water content 60%,Fermentation time continues 40 days; Ferment and can prepare and grow seedlings after rear employing microwave or ultraviolet disinfection airing are extremely driedMatrix.
(4) planting pit dish sowing: should be whole before field planting, apply high-quality decomposed manure according to 2000-in conjunction with ploughing deeply every mu2500 kgs/acre, ploughing depth 15~20cm; Flat planting bedding, selects north-south ridging bedding, row spacing 80cm, row spacing 40cm, ridgeHigh 25~30cm; Field planting require 10cm soil temperature (ground temperature) is stable reach 12 DEG C after field planting, generally in mid or late April field planting, fixedPlant and adopt seeding row spacing 20-30cm, 8000-10000 plant/acre, after field planting, planted and do not water the same day, and next day, disposable irrigating, grew7-10 days phases were watered water one time, and intertill and clean tillage in good time, about the 180 days time of infertility.
(5) decomposed manure is made up of bacterial classification, carrier and auxiliary material; Strain fermentating liquid used is in following ratio compatibility: itsThe component of middle bacterial classification and parts by weight are, streptomyces griseus (Streptomycesgriseus) 20-50 part, Mortierella alpina(Mortierellaalpina) 5-15 part, pseudomonas stanieri (Pseudomonasstutzeri) 5-15 part, marsh is redPseudomonad (Rhodosedoseudomouaspadustris) 20-40 part, the mixed bacteria liquid after four kinds of bacterium compatibilities is fully inhaledInvest carrier; Carrier be wheat, corn or pulse family stalk separately or mixed powder mince, granular size is 1-30mm; Auxiliary material isUrea and farm manure, wherein the weight ratio of urea and farm manure is 1:3; The weight ratio of carrier and auxiliary material is 5:1; By wheat, jadeRice or pulse family powder of straw be broken into 1-30mm section shape, add water to water content 40-70%, then by volume every cubic metre add corruptionRipe fertilizer 100-300g, and mix, at air themperature 20-40 DEG C of lower seal composting; Sealing composting is at air-proof conditionUnder carry out, composting starts turning in latter every 30 days once, when turning moisturizing to water content 40-70%, composting time remaining 60 days; HeapHaving made rear airing can directly be applied in the soil of plantation agate coffee after dry.
The agate coffee seedling medium adopting in the present invention: by wheat, corn stalk powder being broken into the section shape of 1-10mm, add waterTo water content 60-80%, then by volume every cubic metre add urea 2-3kg, sheep excrement and cow dung form according to weight ratio 1:1Farm manure, farm manure by volume every cubic metre add 5-10kg, quick lime by volume every cubic metre add 1-2kg, fermentationComposite bacteria agent capable by volume every cubic metre add 500-1000g, and mix, at 15 DEG C of-40 DEG C of lower seals heaps of air themperatureLong-pending fermentation, sealing heap fermentation is to carry out under plastic covering film or straw screen or mat, fermentation starts turning in latter every 10 days once, when turningMoisturizing is to water content 60%, and fermentation time continues 40 days; Fermented rear employing microwave or ultraviolet disinfection airing to after dryCan prepare seedling medium.
The compoiste fermented microbial inoculum adopting in the present invention is Klebsiella pneumoniae pneumonia subspecies (KlebsiellaPneumoniaesubsppneumonaiae), gel-shaped bacillus (Bacillusmucilaginosus), huge brood cellBacillus (Bacillusmegaterium), bacillus subtilis (Bacillussubtilis) and Trichoderma viride(Trichodermaviride), pseudomonas aeruginosa (Pseudomonasaeruginosa), streptomyces albus(Streptomycesalbus) be mixed with acquisition according to weight ratio 1:1:2:1:2:3:1; In the situation of abundant solid fermentationUnder, the color that wheat, corn stalk powder mince will be from the light yellow dark brown that becomes, and the fermentation heap of crushed material no longer produces and sends out simultaneouslyMould smell, the rear airing of having fermented can prepare matrix after dry.
The decomposed manure using in the agate coffee cultivation adopting in the present invention, decomposed manure is by bacterial classification, carrier and auxiliary materialForm; Strain fermentating liquid used is in following ratio compatibility: wherein the component of bacterial classification and parts by weight are, streptomyces griseus(Streptomycesgriseus) 20-50 part, Mortierella alpina (Mortierellaalpina) 5-15 part, the false unit cell of Si ShiBacterium (Pseudomonasstutzeri) 5-15 part, Rhodopseudomonas palustris (RhodosedoseudomouasPadustris) 20-40 part, the mixed bacteria liquid after four kinds of bacterium compatibilities is fully adsorbed in carrier; Carrier is wheat, corn or pulse familyStalk separately or mixed powder mince, granular size is 1-30mm; Auxiliary material is urea and farm manure, wherein urea and farm manureWeight ratio is 1:3; The weight ratio of carrier and auxiliary material is 5:1; Wheat, corn or pulse family powder of straw are broken into the section shape of 1-30mm,Add water to water content 40-70%, then by volume every cubic metre add decomposed manure 100-300g, and mix, at sky20-40 DEG C of lower seal composting of temperature degree; Sealing composting is to carry out under air-proof condition, and composting starts turning in latter every 30 days once,When turning, moisturizing is to water content 40-70%, composting time remaining 60 days; After composting completes, airing can directly be used after dryIn the soil of plantation agate coffee.
The decomposed manure that the present invention adopts can be is effectively subacidity sandy soil by planting soil improvement, this forHigh and cold high altitude localities, Pamir Mountain Area soil meta-alkalescence is unfavorable for that the realistic problem of agate coffee field planting has important at presentFunction and significance.
In the present invention, selected Klebsiella pneumoniae pneumonia subspecies (KlebsiellapneumoniaesubspPneumonaiae), gel-shaped bacillus (Bacillusmucilaginosus), Bacillus megatherium (BacillusMegaterium), bacillus subtilis (Bacillussubtilis) and Trichoderma viride (Trichodermaviride), copperGreen pseudomonad (Pseudomonasaeruginosa) and streptomyces albus (Streptomycesalbus) are although be all normalThe bacterial classification of seeing, those of ordinary skill in the art can obtain by public's channel, and still, this area has no and adopts above-mentioned seven kinds of bacteriumPlant and adopt mixing composite fermentation to be applied in solid material fermentation, also have no the fermentation being applied in as agate coffee host solid materialIn, although preparation method and the culture medium of selecting in preparation process and the cultivation of the above-mentioned strain fermentating liquid that the present invention adoptsCondition is all that this area is common and know.
In the present invention, selected streptomyces griseus (Streptomycesgriseus), Mortierella alpina(Mortierellaalpina), pseudomonas stanieri (Pseudomonasstutzeri) and Rhodopseudomonas palustris(Rhodosedoseudomouaspadustris), although be all common bacterial classification, repeatedly test through the application of agate coffee land for growing field cropsCard, adopts Pholiota adiposa (Pholiotaadiposa) in the employing decomposing agent that prior art is recorded or decomposed manure formulaAnd flavobacterium columnare (Flavobacteriumcolumnare) and streptomyces griseus (Streptomycesgriseus), high mountainMortierella (Mortierellaalpina), the red false unit cell of pseudomonas stanieri (Pseudomonasstutzeri) and marshBacterium (Rhodosedoseudomouaspadustris) mixed culture fermentation objectively exists bacterial classification mutually to conflict, and obviously exists and usesThe strain combination that former prior art adopts, particularly adopts Pholiota adiposa (Pholiotaadiposa) and flavobacterium columnare(Flavobacteriumcolumnare) bring the unfavorable factor that hardens of soil with the compound mixed culture fermentation of other common bacterial classifications,Being not suitable for the plantation of agate coffee needs the demand of sand matter, and the soil property that the plantation of agate coffee needs should be sandy soil, and the soil is porous. ForThis, the present invention is directed in the decomposing agent of matching in the plantation of agate coffee and can not select Pholiota adiposa (Pholiotaadiposa) and columnFlavobacterium (Flavobacteriumcolumnare) and the compound hybrid solid fermentation of other common bacterial classifications.
In the present invention, bacterial classification streptomyces griseus (Streptomycesgriseus), Mortierella alpina used(Mortierellaalpina), pseudomonas stanieri (Pseudomonasstutzeri) and Rhodopseudomonas palustris(Rhodosedoseudomouaspadustris) zymotic fluid is according to method common in prior art and the preparation of corresponding culture mediumObtain.
Well known, the kind that disease and pest may occur in the plantation of agate coffee mainly contains root footpath borer, leaf causes mouldDeng, the present invention according to the innoxious governance principle taking " cultural control, physical control, biological control are as main, and chemical prevention is auxiliary ",Adopt the interior raw microbacterium of beet (Microbacteriummaritypicum) as Antagonistic Fungi, this bacterial classification is common soil-borne diseaseThe microbial strains of evil, adopts filling root mode to add Antagonistic Fungi suspension, and root irrigation adopts the Antagonistic Fungi of low concentration to spray rootSoil, pouring Antagonistic Fungi viable count is not less than 108The bacteria suspension 10mL of cfu/mL.
The present invention before transplanting, adopts the Antagonistic Fungi of low concentration to spray root matrix soil agate coffee seedling, rejects in time sickStrain, checks that the agate coffee seedling in seedling dish has or not diseased plant, prevents from being transmitted to other strong seedling, strong sprout as found that diseased plant should be rejected in time, makesBecome loss, can take to strengthen and spray the number of times of Antagonistic Fungi or increase the concentration of spraying Antagonistic Fungi in good time.
The present invention carries out strict crop rotation cropping system, and every 1-2 agate coffee plantation and clover plantation crop rotation, according to surveying soil formulaFertilising, enriches the fertilizer fully becoming thoroughly decomposed, and not to fertilize, prevents soil eutrophication.
By implementing the concrete summary of the invention of the present invention, can reach following beneficial effect:
(1) method of agate coffee cultivating and growing provided by the invention is positioned at away from ocean for the Taxkorgan County in XinjiangThe Pamir Mountain Area, for compared with the high altitude mountainous area of high latitude, field irrigation is mainly main by alpine glacier and accumulated snow melt water. Plantation agateThe address choice of coffee is positioned at 38 ° of north latitude, 76 °, eastern footpath, and the plantation of sea level on the average 3000-5500 rice, in height above sea level 4500m PamirHighlands weather severe cold and arid, day and night temperature can reach 28 DEG C. The present invention is according to the biological characteristics of agate coffee, at Xinjiang handkerchief riceUnder the condition of your plateau high latitude, high height above sea level, provide a set of agate coffee high-yield high-efficiency cultivation method, adopt substrate in greenhouse to grow seedlings, greatlyThe method that transplant in field, plants agate coffee in 3500 meters to 4500 meters Pamir Mountain Area severe cold of height above sea level first under dry climate conditionSuccess.
(2), in South America Peru mountain area, agate coffee generally, in sowing in 9~October, after planting approximately carries out thinning in two months, makes plantThere are enough growing spaces. The method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing provided by the invention,The plantation of agate coffee is to nurse young plants in hothouses 3~April, transplants seedlings to land for growing field crops May, can receive by the end of October then to NovemberObtain product, be 7~8 months whole breeding time, shortened 2 months than original producton location Peru; Aspect transplanting, change tradition in the pastThe mode of transplanting seedlings, specification whole ground, making ridge, cultivation seedling technical regulation, summed up the local cultivation seedling technical management measures of a set of adaptation,The same day, cultivation was not watered, next day disposable irrigating, survival rate is more than 98%.
(3) method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing provided by the invention, by adoptingUnify seedling medium with booth and cultivate seedling, decomposing agent fertilizer ridging is used, adopt Antagonistic Fungi to ensure in agate coffee seedling growth periodNutritional need and prevention and elimination of disease and pests, overcome prior art and had no the technology bottle existing for high height above sea level field planting agate coffeeNeck, the cultivation cycle of shortening agate coffee, shorten nearly 2 months than state of original Peru or Yunnan Province of China production agate coffee cycle, reachThe results then of cultivation then. Be 17.5% by detecting agate coffee protein content, polyoses content is 14.3%.
Detailed description of the invention
, for embodiment, the present invention is described below, still, the present invention is not limited to following embodiment.
The supplementary materials such as all Chinese herbal medicines of selecting in the present invention are all well known selecting, but do not limit of the present inventionImplement, in addition, in following explanation, if no special instructions, all % that relate to all refer to m/m mass percent.
Embodiment mono-: the cultivating and growing that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee
Be suitable for the method for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing, % is according to weight percent meter, concreteGrowing and cultivation method is as follows:
(1) agate coffee seed pretreatment: agate coffee seed needs prior to seeding through K cryogenic treatment is preserved at-1 DEG C to-5 DEG C7-10 days with 600 times of liquid of approximately 45% Bravo and 800 times of liquid seed dressings of 55% carbendazol wettable powder, dries standby before growing seedlingsWith; Before sowing, adopt presoaking and germinating processing, seed pulled out and shakeout again with the cold water 16h-24h that at room temperature soaks seed, frequently stir,Soaking seed can be used for growing seedlings.
(2) adopt booth substrate seedling: before sowing, need raise seedling in greenhouse, select raise seedling in greenhouse facility, the reply agate of growing seedlingsDisinfection by ultraviolet light processing is carried out in coffee seed and canopy chamber, creates the environmental condition that is applicable to agate coffee seedling development; Nutritive soil preparationAnd seedbed with soil and seedling medium by weight the ratio of 5:1 fully mix, add 1% plant ash, the soil choosing of ground, seedbedSelect height above sea level 2700m to 3200m, select the leeward plot that faces south, select fertile loose neutral sandy loam, require loose, fertilizer conservation, guarantorWater, by the Nutrition Soil uniform spreading preparing, on seed bed, thickness 10cm-15cm waits to broadcast; Agate coffee is cultivated employing cave, seedbed dish and is educatedSeedling, agate coffee application rate is assessed according to 20~30 grams every mu; Cave dish is fitly emitted on to ground smooth in booth, then waters footWater, canopy chamber will ensure sufficient illumination, annual mid or late Febuary seeding and seedling raising, seedling age can be transplanted after 30~50 days in field planting, oneAs after 7 days agate coffee true leaf grow gradually, after 14 days, true leaf grows to 2~3cm, after 30~50 days, to grow to 6~8cm high for agate coffee true leafTransplant; Adopt during this time the interior raw microbacterium of beet (Microbacteriummaritypicum) as Antagonistic Fungi, to fill with rootMode Antagonistic Fungi suspension adds, and root irrigation adopts according to 1% Antagonistic Fungi sprays root soil, and pouring Antagonistic Fungi viable count is notLower than 108The bacteria suspension of cfu/mL.
(3) planting pit dish sowing: should be whole before field planting, apply high-quality decomposed manure according to 2000-in conjunction with ploughing deeply every mu2500 kgs/acre, ploughing depth 15~20cm; Flat planting bedding, selects north-south ridging bedding, row spacing 80cm, row spacing 40cm, ridgeHigh 25~30cm; Field planting require 10cm soil temperature (ground temperature) is stable reach 12 DEG C after field planting, generally in mid or late April field planting, fixedPlant and adopt seeding row spacing 20-30cm, 8000-10000 plant/acre, after field planting, planted and do not water the same day, and next day, disposable irrigating, grew7-10 days phases were watered water one time, and intertill and clean tillage in good time, about the 180 days time of infertility.
Embodiment bis-: the preparation of agate coffee seedling medium
The agate coffee seedling medium adopting in the present invention: by wheat, corn stalk powder being broken into the section shape of 1-10mm, add waterTo water content 60-80%, then by volume every cubic metre add urea 2-3kg, sheep excrement and cow dung form according to weight ratio 1:1Farm manure 5-10kg, quick lime 1-2kg, compoiste fermented microbial inoculum 500-1000g, and mix, at air themperature 15-40 DEG CSealing heap fermentation, sealing heap fermentation is to carry out under plastic covering film or straw screen or mat, fermentation starts turning in latter every 10 days once,When turning, moisturizing is to water content 60%, and fermentation time continues 40 days; Fermented rear employing microwave or ultraviolet disinfection airing extremelyAfter dry, can prepare seedling medium.
The compoiste fermented microbial inoculum adopting in the present invention is Klebsiella pneumoniae pneumonia subspecies (KlebsiellaPneumoniaesubsppneumonaiae), gel-shaped bacillus (Bacillusmucilaginosus), huge brood cellBacillus (Bacillusmegaterium), bacillus subtilis (Bacillussubtilis) and Trichoderma viride(Trichodermaviride), pseudomonas aeruginosa (Pseudomonasaeruginosa), streptomyces albus(Streptomycesalbus) be mixed with acquisition according to weight ratio 1:1:2:1:2:3:1; In the situation of abundant solid fermentationUnder, the color that wheat, corn stalk powder mince will be from the light yellow dark brown that becomes, and the fermentation heap of crushed material no longer produces and sends out simultaneouslyMould smell, the rear airing of having fermented can prepare matrix after dry.
Embodiment tri-: the preparation of decomposed manure in the cultivation of agate coffee
The decomposed manure using in the agate coffee cultivation adopting in the present invention, decomposed manure is by bacterial classification, carrier and auxiliary materialForm; Strain fermentating liquid used is in following ratio compatibility: wherein the component of bacterial classification and parts by weight are, streptomyces griseus(Streptomycesgriseus) 20-50 part, Mortierella alpina (Mortierellaalpina) 5-15 part, the false unit cell of Si ShiBacterium (Pseudomonasstutzeri) 5-15 part, Rhodopseudomonas palustris (RhodosedoseudomouasPadustris) 20-40 part, the bacterium liquid after compatibility is fully adsorbed in carrier; Carrier be wheat, corn or pulse family stalk separately orPerson's mixed powder minces, and granular size is 1-30mm; Auxiliary material is urea and farm manure, and wherein the weight ratio of urea and farm manure is 1:3; The weight ratio of carrier and auxiliary material is 5:1; The section shape that wheat, corn or pulse family powder of straw is broken into 1-30mm, adds water to moistureAmount 40-70%, then by volume every cubic metre add decomposed manure 100-300g, and mix, at air themperature 20-40 DEG C of lower seal compostings; Sealing composting is to carry out under air-proof condition, and composting starts turning in latter every 30 days once, moisturizing when turningTo water content 40-70%, composting time remaining 60 days; After composting completes, airing can directly be applied to soil after dry.
The decomposed manure that the present invention adopts can be is effectively subacidity sandy soil by planting soil improvement, this forHigh and cold high altitude localities, Pamir Mountain Area soil meta-alkalescence is unfavorable for that the realistic problem of agate coffee field planting has important at presentFunction and significance.
In the present invention, selected Klebsiella pneumoniae pneumonia subspecies (KlebsiellapneumoniaesubspPneumonaiae), gel-shaped bacillus (Bacillusmucilaginosus), Bacillus megatherium (BacillusMegaterium), bacillus subtilis (Bacillussubtilis) and Trichoderma viride (Trichodermaviride), copperGreen pseudomonad (Pseudomonasaeruginosa), streptomyces albus (Streptomycesalbus) are although be all commonBacterial classification, those of ordinary skill in the art can obtain by public's channel, still, this area have no adopt above-mentioned seven kinds of bacterial classificationsAdopt mixing composite fermentation to be applied in solid material fermentation, also have no in the fermentation that is applied in crops host solid material,The preparation method of the above-mentioned strain fermentating liquid that meanwhile, the present invention adopts and the culture medium of selecting in preparation process thereof and cultivation barPart is all that this area is common and know.
In the present invention, selected streptomyces griseus (Streptomycesgriseus), Mortierella alpina(Mortierellaalpina), pseudomonas stanieri (Pseudomonasstutzeri) and Rhodopseudomonas palustris(Rhodosedoseudomouaspadustris), although be all common bacterial classification, repeatedly test through the application of agate coffee land for growing field cropsCard, adopts Pholiota adiposa (Pholiotaadiposa) in the employing decomposing agent that prior art is recorded or decomposed manure formulaAnd flavobacterium columnare (Flavobacteriumcolumnare) and streptomyces griseus (Streptomycesgriseus), high mountainMortierella (Mortierellaalpina), the red false unit cell of pseudomonas stanieri (Pseudomonasstutzeri) and marshBacterium (Rhodosedoseudomouaspadustris) mixed culture fermentation objectively exists bacterial classification mutually to conflict, and obviously exists and usesThe strain combination that former prior art adopts, particularly adopts Pholiota adiposa (Pholiotaadiposa) and flavobacterium columnare(Flavobacteriumcolumnare) bring the unfavorable factor that hardens of soil with the compound mixed culture fermentation of other common bacterial classifications,Being not suitable for the plantation of agate coffee needs the demand of sandy soil, and the soil property that the plantation of agate coffee needs should be sandy soil, and the soil is porous. ForThis, the present invention is directed in the decomposing agent of matching in the plantation of agate coffee and can not select Pholiota adiposa (Pholiotaadiposa) and columnFlavobacterium (Flavobacteriumcolumnare) and the compound hybrid solid fermentation of other common bacterial classifications.
In the present invention, bacterial classification streptomyces griseus (Streptomycesgriseus), Mortierella alpina used(Mortierellaalpina), pseudomonas stanieri (Pseudomonasstutzeri) and Rhodopseudomonas palustris(Rhodosedoseudomouaspadustris) zymotic fluid is according to method common in prior art and the preparation of corresponding culture mediumObtain.
The kind that disease and pest may occur in the plantation of agate coffee mainly contains root footpath borer, leaf causes mould etc., and the present invention pressesAccording to the innoxious governance principle taking " cultural control, physical control, biological control are as main, and chemical prevention is auxiliary ", adopt in beetRaw microbacterium (Microbacteriummaritypicum) is as Antagonistic Fungi, and this bacterial classification is the microorganism of common soil-borne diseaseBacterial strain, adopts filling root mode to add Antagonistic Fungi suspension, and root irrigation adopts the Antagonistic Fungi of low concentration to spray root soil, waters short of moneyAntibacterial viable count is not less than 108The bacteria suspension 10mL of cfu/mL.
The present invention before transplanting, adopts the Antagonistic Fungi of low concentration to spray root matrix soil agate coffee seedling, rejects in time sickStrain, checks that the agate coffee seedling in seedling dish has or not diseased plant, prevents from being transmitted to other strong seedling, strong sprout as found that diseased plant should be rejected in time, makesBecome loss, can take to strengthen and spray the number of times of Antagonistic Fungi or increase the concentration of spraying Antagonistic Fungi in good time.
By the enforcement of above-described embodiment, the Taxkorgan County that the present invention is directed to Xinjiang is positioned at the Pamir away from oceanPlateau, for compared with the high altitude mountainous area of high latitude. Field irrigation is mainly main by alpine glacier and accumulated snow melt water. The ground of plantation agate coffee38 ° of north latitude, 76 °, eastern footpath are selected to be positioned in location, and the plantation of sea level on the average 3000-5500 rice, on ground, the height above sea level 4500m Pamir Mountain AreaDistrict's weather severe cold and arid, surface soil alkalinity, day and night temperature can reach 28 DEG C. The present invention is directed to the biological characteristics of agate coffee, in XinjiangUnder the condition of high latitude, high height above sea level, provide a set of agate coffee high-yield high-efficiency cultivation method, employing substrate in greenhouse is grown seedlings, field-transplantingMethod, under dry climate condition, plant first agate coffee success in 3500 meters to 4500 meters Pamir Mountain Area severe cold of height above sea level.
In South America Peru mountain area, agate coffee generally, in sowing in 9~October, after planting approximately carries out thinning in two months, and plant is hadEnough growing spaces. During field management, note carrying out the work such as liquid manure supply and the prevention and control of plant diseases, pest control, 5~July of next year,In the time that fleshy root diameter grows to about 5cm, gather in the crops, need 9~10 months whole breeding time. Applicable Pamir provided by the invention is highThe method of former high and cold high altitude localities agate coffee cultivating and growing, the plantation of agate coffee is to nurse young plants in hothouses 3~April, May is by seedlingBe transplanted to land for growing field crops, can gather in the crops product by the end of October then to November, be 7~8 months whole breeding time, contracts than original producton location PeruShort 2 months; Aspect transplanting, changed in the past traditional mode of transplanting seedlings, specification whole ground, making ridge, cultivation seedling technical regulation, alwaysTied the local cultivation seedling technical management measures of a set of adaptation, the same day, cultivation was not watered, next day disposable irrigating, survival rate is more than 98%.
The present invention cultivates seedling by adopting booth to unify seedling medium, and the ridging of decomposing agent fertilizer is used, and adopts antagonismBacterium ensures nutritional need and the prevention and elimination of disease and pests in agate coffee seedling growth period, has overcome existing having no for high height above sea level land for growing field crops kindPlant the technical bottleneck that agate coffee exists, shorten the cultivation cycle of agate coffee, produce the contracting of agate coffee cycle than state of original Peru or Yunnan Province of ChinaShort nearly 2 months, reach the results then of cultivation then. Be 17.5% by detecting agate coffee protein content, polyoses content is14.3%。
Claims (1)
1. a method that is suitable for high and cold high altitude localities, Pamir Mountain Area agate coffee cultivating and growing, is characterized in that, % is according to weightPercentage meter, concrete growing and cultivation method is as follows:
(1) agate coffee seed pretreatment: agate coffee seed needs prior to seeding through K cryogenic treatment is preserved 7-10 at-1 DEG C to-5 DEG CMy god, before growing seedlings, with 600 times of liquid of 45% Bravo and 800 times of liquid seed dressings of 55% carbendazol wettable powder, dry for subsequent use; Before sowingAdopt presoaking and germinating processing, seed is pulled out and shakeout again with the cold water 16h-24h that at room temperature soaks seed, frequently stir, soak seedBe used for growing seedlings;
(2) adopt booth substrate seedling: before field planting, need raise seedling in greenhouse, select raise seedling in greenhouse facility, before growing seedlings, tackle agate coffee kindDisinfection by ultraviolet light processing is carried out in son and canopy chamber, creates the environmental condition that is applicable to agate coffee seedling development; Nutritive soil preparation is with soilEarth and the seedling medium by weight ratio of 5:1 fully mix, and add 1% plant ash, and height above sea level 2700m is selected extremely in ground, seedbed3200m, selects the leeward plot that faces south, and selects fertile loose neutral sandy loam, requires loose, fertilizer conservation, water conservation, by what prepareNutrition Soil uniform spreading is on seed bed, and thickness 10cm-15cm waits to broadcast; Agate coffee is cultivated seedbed and is adopted hole plate seedling growth, agate coffee application rateAssess according to 20~30 grams every mu; Cave dish is fitly emitted on to ground smooth in booth, then waters sufficient water, canopy chamber will ensureSufficient illumination, annual mid or late Febuary seeding and seedling raising, seedling age after 30~50 days field planting transplant, after 7 days, agate coffee true leaf is long graduallyGo out, after 14 days, true leaf grows to 2~3cm, and after 30~50 days, agate coffee true leaf grows to that 6~8cm is high to transplant; Adopt during this time beetInterior raw microbacterium (Microbacteriummaritypicum), as Antagonistic Fungi, adds to fill with root mode Antagonistic Fungi suspension, fills withRoot is processed the Antagonistic Fungi sprinkling root matrix soil that adopts 1%, and pouring Antagonistic Fungi concentration is 108The bacteria suspension of cfu/mL;
(3) field planting: should be whole before field planting, apply high-quality decomposed manure 2000-2500 kilogram, ploughing depth in conjunction with ploughing deeply every mu15~20cm; Flat planting bedding, selects north-south ridging bedding, row spacing 80cm, row spacing 40cm, the high 25~30cm in ridge; Field planting requirement10cm soil temperature is stable reach 12 DEG C after field planting, in mid or late April field planting, field planting adopts seeding row spacing 20-30cm, 8000-10000 plants/acre, after field planting, do not water the same day, and next day, disposable irrigating, watered water 7-10 days growth periods one time, and intertillage in good time removesGrass, about the 180 days time of infertility;
Described seedling medium, by wheat, corn stalk powder being broken into the section shape of 1-10mm, adds water to water content 60-80%, soAdd urea 2-3kg by every cubic metre afterwards, sheep excrement and cow dung are according to weight ratio 1:1 composition farm manure 5-10kg, quick lime 1-2kg, compoiste fermented microbial inoculum 500-1000g, and mix, at air themperature 15-40 DEG C of lower seal heap fermentation, sealing is piled upFermentation is to carry out under plastic covering film or straw screen or mat, and fermentation starts turning in latter every 10 days once, and when turning, moisturizing is to water content60%, fermentation time continues 40 days; Fermented rear employing microwave or ultraviolet disinfection, airing is educated to preparing after drySeedling matrix;
Described compoiste fermented microbial inoculum is Klebsiella pneumoniae pneumonia subspecies (KlebsiellapneumoniaesubspPneumonaiae), gel-shaped bacillus (Bacillusmucilaginosus), Bacillus megatherium (BacillusMegaterium), bacillus subtilis (Bacillussubtilis), Trichoderma viride (Trichodermaviride), verdigrisPseudomonad (Pseudomonasaeruginosa) and streptomyces albus (Streptomycesalbus) are according to weight ratio 1:1:2:1:2:3:1 is mixed with acquisition;
Described decomposed manure is made up of bacterial classification, carrier and auxiliary material; Bacterial classification used is in following ratio proportioning: wherein bacterial classificationComponent and parts by weight are, streptomyces griseus (Streptomycesgriseus) 20-50 part, Mortierella alpina(Mortierellaalpina) 5-15 part, pseudomonas stanieri (Pseudomonasstutzeri) 5-15 part, marsh is redPseudomonad (Rhodosedoseudomouaspadustris) 20-40 part, the mixed bacteria liquid after four kinds of bacterium proportionings is fully inhaledInvest carrier; Carrier be wheat, corn or pulse family stalk separately or mixed powder mince, granular size is 1-30mm; Auxiliary material isUrea and farm manure, wherein the weight ratio of urea and farm manure is 1:3; The weight ratio of carrier and auxiliary material is 5:1; By wheat, jadeRice or pulse family powder of straw are broken into the section shape of 1-30mm, add water to water content 40-70%; Then by volume every cubic metre add corruptionRipe fertilizer 100-300g, and mix, at air themperature 20-40 DEG C of lower seal composting; Sealing composting is at air-proof conditionUnder carry out, composting starts turning in latter every 30 days once, when turning moisturizing to water content 40-70%, composting time remaining 60 days; HeapMade rear airing obtains after dry.
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