CN103919140B - A kind of perilla herb oil microballoon and preparation method thereof - Google Patents

Abstract

The invention provides a perilla oil microsphere and a preparation method thereof. The prepared perilla oil microsphere has uniform shape and smooth surface. The preparation method is to use acetic acid solution (1% v/v) to prepare acid-processed gelatin (type A) uniform solution with pH 4-5 and hydroxypropyl distarch phosphate solution with pH 4-5 for standby; Add polyglycerol fatty acid ester to the oil and stir for 30 minutes, add dropwise to the prepared gelatin solution, stir and cut evenly to obtain a uniform emulsion; add prepared hydroxypropyl distarch to the emulsion The phosphate ester solution was stirred and then left to stand, centrifuged at 10,000 rpm at 25°C, and dried under negative pressure at room temperature to obtain perilla oil microspheres, which were wrapped in gelatin. The preparation method has mild conditions, does not use organic solvents, and has low ion concentration.

Description

A kind of perilla oil microsphere and preparation method thereof

technical field

The invention relates to the technical field of food oil, in particular to an oil microsphere and a preparation method thereof.

Background technique

Perilla oil is the oil obtained by pressing the dried ripe fruit of perilla plant of Labiatae. Perilla oil is rich in α-linolenic acid (accounting for 62 - 85% of total fatty acids), which is mainly in the form of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) after metabolism in the human body exist. It has been found through research that perilla oil has anti-thrombotic, anti-allergic, anti-inflammatory effects, lowering blood pressure, lowering blood lipids, lowering cholesterol, anti-cancer effects, promoting effects on learning behavior activities and visual functions, antitussive and asthma-relieving effects, and antibacterial effect.

On the other hand, due to the high content of unsaturated fatty acids in perilla oil, perilla oil has the disadvantages of being easily oxidized and having a bad mouthfeel. People have tried methods such as microcapsule embedding and nitrogen filling protection, and have achieved certain results, but there are some limitations. With the development of microcapsule preparation technology, nano-microsphere technology with smaller particle size and mild operating conditions has emerged. However, after reviewing the literature, there is no relevant report on perilla oil microspheres in China. The present invention utilizes the characteristics of acid-processed gelatin (type A) and hydroxypropyl distarch phosphate having opposite charges under the condition of pH 4-5, and both are molecular polymers, through ion coagulation, steric hindrance and dehydration, Form microspheres. The preparation method of the method has mild conditions, does not use organic solvents, and has low ion concentration.

Contents of the invention

The object of the present invention is to provide a perilla oil microsphere and a preparation method thereof, the perilla oil microsphere prepared by the method improves the stability of the perilla oil and increases the bioavailability of the perilla oil. The perilla oil microspheres prepared by this method have fine particle size, smooth surface and good embedding.

A kind of perilla oil microsphere and its preparation method, it is characterized in that, the homogeneous solution (0.05%-0.50% % ), and pH 4-5 hydroxypropyl distarch phosphate solution (0.2%–1.5%) for later use; add polyglycerol fatty acid ester (0.025%–0.225%) to a certain volume of perilla oil while stirring, 50 Stir in a water bath at ℃ for 30 minutes, add dropwise to the prepared gelatin solution (the ratio of perilla oil to gelatin is 3:1 -1:5, v/w), stir for 30 minutes and keep stirring at 1000 – 2000 r/min Cut evenly at a rotating speed to obtain a uniform emulsion; add the prepared hydroxypropyl distarch phosphate solution to the emulsion, stir for 20 minutes, then stand for 3-8 hours, and centrifuge at 10,000 rpm for 10 minutes at 25°C. The particles formed were collected and the resulting wet particles were dispersed in distilled water. Then dry at room temperature under negative pressure, and store under dry conditions at 25°C. The core material of the microsphere is perilla oil, and the wrapper is gelatin. The preparation method has mild conditions, does not use organic solvents, and has low ion concentration.

The specific implementation method Embodiment 1:

Prepare pH 4 acid-processed gelatin (Type A) homogeneous solution (0.05%) and pH 4 hydroxypropyl distarch phosphate solution (0.5%) with acetic acid solution (1% v/v) at room temperature Standby; add polyglycerol fatty acid ester (0.025%) to a certain volume of perilla oil while stirring, stir in a water bath at 50°C for 30 minutes, and add dropwise to the prepared gelatin solution (the ratio of perilla oil to gelatin is 1:1, v/w), stir for 30 minutes and shear evenly at 1500 r/min to obtain a uniform emulsion; add the prepared hydroxypropyl distarch phosphate solution to the emulsion, and stir for 20 minutes , and then left to stand for 4 hours, centrifuged at 10,000 rpm for 10 minutes at 25°C to collect the formed particles, and the obtained wet particles were dispersed in distilled water. Then dry at room temperature under negative pressure, and store under dry conditions at 25°C. The core material of the microsphere is perilla oil, and the wrapper is gelatin. The preparation method has mild conditions, does not use organic solvents, and has low ion concentration.

Example 2:

At room temperature, use acetic acid solution (1% v/v) to prepare acid-process gelatin (type A) homogeneous solution of pH 5 (0.09%), and hydroxypropyl distarch phosphate solution (0.8%) of pH 5 for later use; Add polyglycerol fatty acid ester (0.05%) to a certain volume of perilla oil with stirring, stir in a water bath at 50°C for 30 minutes, and add dropwise to the prepared gelatin solution (the ratio of perilla oil to gelatin is 1:3 , v/w), stirred for 30 minutes and sheared evenly at 2000 r/min to obtain a uniform emulsion; added the prepared hydroxypropyl distarch phosphate solution to the emulsion, stirred for 20 minutes, and then statically Place for 6 hours, centrifuge at 10,000 rpm for 10 minutes at 25°C, collect the formed particles, and disperse the obtained wet particles in distilled water. Then dry at room temperature under negative pressure, and store under dry conditions at 25°C. The core material of the microsphere is perilla oil, and the wrapper is gelatin. The preparation method has mild conditions, does not use organic solvents, and has low ion concentration.

Claims (1)

1. a perilla herb oil microballoon, it is characterized in that, preparation method is: the hydroxypropyl PASELLI EASYGEL solution for standby at room temperature using acid process gelatin (A type) homogeneous solution of the acetic acid solutions pH 4-5 of 1 % v/v, 0.05 % – 0.50 % and pH4-5,0.2 % – 1.5 % respectively; 0.025 % – 0.225 % polyglyceryl fatty acid ester is added under stirring in the perilla herb oil of certain volume, stir 30 minutes in 50 DEG C of water-baths, the ratio being 3:1-1:5 v/w according to perilla herb oil and gelatin is dropwise added in the gelatin solution prepared, stir 30 minutes and at 1000 – 2000 r/min rotating speed down cuts evenly, obtain homogeneous emulsion; In emulsion, add the hydroxypropyl PASELLI EASYGEL solution prepared, stir 20 minutes, then leave standstill 3-8 hour, the centrifugal 10min of 10000rpm at 25 DEG C, collect the particle formed, the wet particle dispersion obtained is in distilled water; Then dry under room temperature negative pressure, preserve under 25 DEG C of drying conditions.