CN103918418B - The storage device of Seeds of Dendrobium Candidum and method for preserving thereof - Google Patents
The storage device of Seeds of Dendrobium Candidum and method for preserving thereof Download PDFInfo
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Abstract
The invention belongs to Seed Deposit technical field, relate to a kind of storage device and method for preserving thereof of Seeds of Dendrobium Candidum, storage device comprises depository dish and is placed in the seed collection bottle of the storage Seeds of Dendrobium Candidum in depository dish, collect in seed collection bottle and have Seeds of Dendrobium Candidum, bottle cap covers after on bottleneck, the outer surface of bottle cap and bottleneck junction is provided with ventilative sealed membrane, the jack of receiving flask dispenser is laid seed collection bottle, method for preserving has disinfecting utensils, capsule is sterilized, the collection of seed, stored refrigerated four steps, advantage is: storage device reasonable in design of the present invention, method for preserving science is reliable, the preservation time is long, survival rate is high, the requirement that dendrobium candidum organizes training throughout the year can be met, be applicable to collection and the preservation of similar seed.
Description
Technical field
The invention belongs to Seed Deposit technical field, refer in particular to a kind of storage device and method for preserving thereof of Seeds of Dendrobium Candidum.
Background technology
Dendrobium candidum (DendrobiumofficinaleKimuraetMigo) is the orchid family Dendrobium herbaceos perennial, one of the Pharmacopoeia of the People's Republic of China 5 kinds of Dendrobium Sws of including, the effects such as antitumor, anti-ageing, acoustic resistive band is tired because having, hemangiectasis and enhancing body immunity, be called as the celestial grass of help, rank first of Chinese nine immortal grass.
Dendrobium candidum principle active component is stem of noble dendrobium class polysaccharide and dendrobine etc.The water-soluble polysaccharide that dendrobium candidum includes is a para-immunity reinforcing agent, has stronger anti-tumor biological, can promote that lymphocyte produces lymphokine, strengthen body humoral immune function, cellular immune function, strengthen body autoimmunity.The pharmacological action of dendrobine is mainly manifested in antitumor, aspect of bringing down a fever etc. to cardiovascular and intestines and stomach inhibitory action and pain relieving.There are some researches show, the dendrobine content in dendrobium candidum is not high, but quality is better.Dendrobium candidum contains abundant Essential metal elements of human, and wherein Zn, Cu, Fe, Mn, Sr content is higher, and these trace elements have good regulating action for the raising of human mechanism activity.Free amino acid is also one of principle active component in the stem of noble dendrobium.The primary amino acid of dendrobium candidum is aspartic acid, glutamic acid, sweet ammonia, valine and leucine, and this 5 seed amino acid accounts for 53% of total amino acid.Also containing 2 kinds of luxuriant and rich with fragrance compounds in dendrobium candidum: chrysotoxene and luxuriant blue element, these two kinds of compounds have antitumor activity.
Current dendrobium candidum is mainly used in that fresh goods is edible, dry product and converted products thereof and clinical practice aspect.
Dendrobium candidum is as edible mainly its fresh bar edible of fresh goods, and eating method comprises: chewing is instant, it is edible to squeeze the juice, make tea, enter meals, infusing drugs in wine.Fresh produce dendrobium flowers is except drying tea making, and can also work as dish use, arrange in pairs or groups in recipe, mouthfeel is fresh and crisp good to eat.
The dendrobium candidum circulated in the market is dry product and converted products thereof mainly, i.e. 2nd generation product.Mainly contain the series of products such as Tiepi Fengdou, dendrobium candidum particle electuary, dendrobium officinale flower tea, dendrobium candidum tealeaves, officinal Dendrobium stem capsule and dendrobium candidum functional beverage.
Dendrobium candidum is used for treating chronic pharyngitis, digestive system disease, ophthalmology disease, the complete plug disease of thrombus, arthritis clinically, and the treatment of cancer or supplemental treatment, particularly in recent years for eliminating the side effect after cancer radiation, chemotherapy and recovering physical efficiency successful.Dendrobium candidum is generally processed into Chinese patent drug on medical, as stem of noble dendrobium particle, Shiyu Yeguang Wan, MAILUONING ZHUSHEYE, TONGSAIMAI PIAN and yin-nourishing oral liquid etc.
Dendrobium candidum is generally bloomed in 5 ~ June, forms capsule after pollination.Its seed is minimum, and have several ten thousand seeds in a capsule, normotrophic seed, is similar to olive shape, and seed coat is the parenchyma cell of layer of transparent, and there is a spherical embryo centre, the cell number of embryo, approximately remains on more than 90 cell.
The reproductive survival rate that plantation Seeds of Dendrobium Candidum carries out dendrobium candidum is low, and the method for mostly employing group training now carries out the breeding of dendrobium candidum, but the preservation of Seeds of Dendrobium Candidum is the key realizing a large amount of tissue culture propagation of dendrobium candidum.
At present, the preservation of Seeds of Dendrobium Candidum, general employing Cord blood, the capsule of dendrobium candidum is picked, after full crackless capsule is put into the bag that silica gel is housed, sack is put in refrigerator and preserve, like this, the holding time of Seeds of Dendrobium Candidum can not be very long, about 3-5 months, the raw slight crack because the capsule shell of dendrobium candidum is easily gone bankrupt, and the seed of dendrobium candidum is very tiny, so be easy to drop from breach, conventional method is preserved, and can not meet the requirement that dendrobium candidum organizes training throughout the year.
Summary of the invention
The object of this invention is to provide a kind of storage device and method for preserving thereof of Seeds of Dendrobium Candidum.
The object of the present invention is achieved like this:
The storage device of Seeds of Dendrobium Candidum, comprise depository dish and be placed in the seed collection bottle of the storage Seeds of Dendrobium Candidum in depository dish, the concrete structure of described depository dish is: be installed with on the opening of box body with some jacks receiving flask dispenser and cover the lid on box opening, four angles of box body lower surface are provided with the projection stretched out downwards, four angles of lid upper surface are provided with the bulge loop of the be set with projection protruded upward, the concrete structure of described seed collection bottle is: top is cylindricality, bottom be the bottle of taper opening on be connected with bottle cap by softer and thin brace, the outer surface of the annular protrusion on bottle cap lower surface and the inner surface clamping of bottleneck, outside annular protrusion, the bottle cap of brace offside is provided with the card be connected on bottleneck outer surface, collect in seed collection bottle and have Seeds of Dendrobium Candidum, bottle cap covers after on bottleneck, the outer surface of bottle cap and bottleneck junction is provided with ventilative sealed membrane, the jack of receiving flask dispenser is laid seed collection bottle.
Above-mentioned sealed membrane is PM-996 sealed membrane or INVENTIONPaper filter film or PTFE bacteria filters.
Above-mentioned Seeds of Dendrobium Candidum is the corpuscular seed after removing capsule shell.
Above-mentioned seed collection bottle polypropylene material is made.
The inner surface of the opening of above-mentioned box body is provided with a ring annular groove, described receiving flask dispenser is installed on described cannelure, the upper surface of receiving flask dispenser stretches out the upper surface of box opening, and the downside of described lid is fastened on the upper surface of box opening and stretches out on the lateral surface of receiving flask dispenser of box opening upper surface.
Utilize the method for preserving of storage device to Seeds of Dendrobium Candidum of above-mentioned Seeds of Dendrobium Candidum, completed by following steps:
(1) disinfecting utensils: put into overall disinfection after cup together with tweezers, scissors after seed collection bottle is opened bottle cap, put on superclean bench for subsequent use after sterilization;
(2) capsule sterilization: by full crackless dendrobium candidum capsule, alcohol-pickled 1 minute that is placed on 70% ~ 75% on superclean bench with the dust that sterile water carefully rinses on capsule, then sterilize 6 ~ 10 minutes with the mercuric chloride of 1g/L, finally use aseptic water washing 3 ~ 5 times, blot the moisture on capsule with aseptic filter paper;
(3) collection of seed: the base portion capsule disinfected being clamped in superclean bench capsule with tweezers, cut off with the top of scissors by capsule, the bottleneck of seed collection bottle is placed on bottle holder upward, the clip of capsule aims at the bottleneck of seed collection bottle down, beat the tweezers clamping capsule gently, seed is all poured in seed collection bottle, after the bottle cap of seed collection bottle is built, sealed with sealed membrane;
(4) stored refrigerated: the jack that the receiving flask dispenser of depository dish put into by the seed collection bottle that Seeds of Dendrobium Candidum is housed that sealing step (3) completed is good covers lid, moves into refrigeration in refrigerating chamber.
Above-mentioned refrigerating chamber is refrigerator, and the temperature of described refrigeration is 2-10 DEG C.
Above-mentioned refrigerating chamber is refrigerator, and the temperature of described refrigeration is 3 DEG C or 4 DEG C or 5 DEG C.
The present invention gives prominence to compared to existing technology and useful technique effect is:
1, the present invention utilizes depository dish and seed collection bottle to carry out seed collection and low-temperature preservation to the dendrobium candidum capsule through disinfecting, preservation was for up to more than 10 years, and survival rate is high, only can compare the preservation time of 3-5 months compared to existing technology, the preservation time improves tens times, main cause: one is aseptic collection seed; Two is that seed collection bottle can not break; Three are and sealed membrane ensure that the eupnea of seed in seed collection bottle; Four is long-time preservations that the refrigerated storage temperature of about 4 DEG C is suitable for seed; Five is the refrigerators that have employed special depository dish, seed collection bottle and refrigerate.
2, storage device reasonable in design of the present invention, method for preserving science is reliable, and the preservation time is long, and survival rate is high, can meet the requirement that dendrobium candidum organizes training throughout the year, is applicable to collection and the preservation of similar seed.
Accompanying drawing explanation
Fig. 1 is the schematic perspective view of depository dish of the present invention.
Fig. 2 is the cutaway view that depository dish adduction of the present invention is placed with seed collection bottle.
Fig. 3 is the cutaway view after multiple depository dish mounted on top of the present invention.
Fig. 4 is the schematic perspective view of seed collection bottle of the present invention.
Fig. 5 is the cutaway view of seed collection bottle of the present invention, is loaded with Seeds of Dendrobium Candidum in it.
Embodiment
Below in conjunction with accompanying drawing, with specific embodiment, the invention will be further described, see Fig. 1-Fig. 5:
The storage device of Seeds of Dendrobium Candidum, comprise the seed collection bottle 20 that depository dish 10 and the storage be placed in depository dish 10 have Seeds of Dendrobium Candidum 30, the concrete structure of described depository dish 10 is: on the opening of box body 11, be installed with the receiving flask dispenser 15 with some jacks 16 and the lid 12 that covers on box body 11 opening, four angles of box body 11 lower surface are provided with the projection 14 stretched out downwards, four angles of lid 12 upper surface are provided with the bulge loop 13 of the be set with projection 14 protruded upward, the concrete structure of described seed collection bottle 20 is: top 21a is cylindricality, bottom 21a be the bottle 21 of taper bottleneck 28 on be connected with bottle cap 25 by softer and thin brace 24, the outer surface of the annular protrusion 27 on bottle cap 25 lower surface and the inner surface clamping of bottleneck 28, outside annular protrusion 27, the bottle cap 25 of brace 24 offside is provided with the card 26 be connected on bottleneck 28 outer surface, collect in seed collection bottle 20 and have Seeds of Dendrobium Candidum 30, bottle cap 25 covers after on bottleneck 28, bottle cap 25 is provided with ventilative sealed membrane 40 with the outer surface of bottleneck 28 junction, the jack 16 of receiving flask dispenser 15 lays seed collection bottle 20.
Above-mentioned sealed membrane 40 is PM-996 sealed membrane or INVENTIONPaper filter film or PTFE bacteria filters.
Above-mentioned Seeds of Dendrobium Candidum 30 is the corpuscular seeds after removing capsule shell.
Above-mentioned seed collection bottle 20 is made with polypropylene material.
The inner surface of the opening of above-mentioned depository dish 10 is provided with a ring annular groove 111, described receiving flask dispenser 15 is installed on described cannelure 111, the upper surface of receiving flask dispenser 15 stretches out the upper surface of box body 11 opening, and the downside of described lid 12 is fastened on the upper surface of box opening and stretches out on the lateral surface 151 of receiving flask dispenser 15 of box opening upper surface.
Utilize the method for preserving of storage device to Seeds of Dendrobium Candidum of above-mentioned Seeds of Dendrobium Candidum, completed by following steps:
(1) disinfecting utensils: put into overall disinfection after cup together with tweezers, scissors after seed collection bottle 20 is opened bottle cap 25, put on superclean bench for subsequent use after sterilization;
(2) capsule sterilization: by full crackless dendrobium candidum capsule, alcohol-pickled 1 minute that is placed on 70% ~ 75% on superclean bench with the dust that sterile water carefully rinses on capsule, then sterilize 6 ~ 10 minutes with the mercuric chloride of 1g/L, finally use aseptic water washing 3 ~ 5 times, blot the moisture on capsule with aseptic filter paper;
(3) collection of seed: the base portion capsule disinfected being clamped in superclean bench capsule with tweezers, cut off with the top of scissors by capsule, the bottleneck 28 of seed collection bottle 20 is placed on bottle holder upward, the clip of capsule aims at the bottleneck 28 of seed collection bottle 20 down, beat the tweezers clamping capsule gently, seed is all poured in seed collection bottle, after the bottle cap 25 of seed collection bottle 20 is built, seal with the junction outer surface of bottle cap 25 with the bottleneck 28 of sealed membrane 40 by its seed collection bottle 20;
(4) stored refrigerated: the jack 16 that the receiving flask dispenser 15 of depository dish 10 put into by the seed collection bottle 20 that Seeds of Dendrobium Candidum 30 is housed that sealing step (3) completed is good covers lid 12, move into refrigeration in refrigerating chamber, by the mounted on top of multiple depository dish 10, and certain airspace can be remained with between adjacent depository dish 10 in the bulge loop 13 utilizing the projection 14 of box body 11 lower surface of a depository dish 10 to insert lid 12 upper surface of another depository dish 10.
Above-mentioned refrigerating chamber is refrigerator, and the temperature of described refrigeration is 2-10 DEG C.
Above-mentioned refrigerating chamber is refrigerator, and the temperature of described refrigeration is 3 DEG C or 4 DEG C or 5 DEG C.
PM (Parafilm) sealed membrane is translucent, have flexible, thermoplastic, high performance water proof material, this is as colourless, odorless, " (0.127mm), has softness-plasticity-prevent water loss; automatic-sealed-tasteless-easily cut, the characteristic of thermoplastic-translucent-colourless for tasteless, thickness only 0.005.
PM sealed membrane has the damp proof performance of waterproof; the volatilization of effective prevention sample moisture and pollution; available protecting anhydrous substances; be wrapped on sharp-pointed profile object and also can not be torn; there is excellent toughness; also can stretch close to the length of twice when 21 DEG C, raise, around toughness lifting corresponding to pliability with temperature.
PTFE bacteria filters is a kind of group training dedicated film.Its microporous bodies network structure adopts the expanded stretching of low-molecular-weight PTFE resin and obtains.Percent opening reaches more than 85%.Aperture 0.3 μ.Heatproof can reach 300 DEG C, has again high elasticity and outstanding hydrophobicity.Unique water-proof breathable properties makes pollution rate be reduced to zero.Have an unexpected effect as bacteriological filter material at engineering field.Counterdie adopts PP non-stretched film, thickness 0.06mm.Light transmittance reaches more than 85%.Utilize general character principle, by high-frequency welding technology, PTFE film is welded on PP film firmly.Thus reach the fungi-proofing object of sealing.
Above-described embodiment is only preferred embodiment of the present invention, not limits the scope of the invention according to this, therefore: all equivalence changes done according to structure of the present invention, shape, principle, all should be covered by within protection scope of the present invention.
Claims (4)
1. the method for preserving of a Seeds of Dendrobium Candidum, it is characterized in that: adopt the storage device of Seeds of Dendrobium Candidum to carry out preservation, the storage device of described Seeds of Dendrobium Candidum comprises depository dish and the storage be placed in depository dish has the seed collection bottle of Seeds of Dendrobium Candidum, the concrete structure of described depository dish is: be installed with on the opening of box body with some jacks receiving flask dispenser and cover the lid on box opening, four angles of box body lower surface are provided with the projection stretched out downwards, four angles of lid upper surface are provided with the bulge loop of the be set with projection protruded upward, the concrete structure of described seed collection bottle is: top is cylindricality, bottom be the bottle of taper opening on be connected with bottle cap by softer and thin brace, the outer surface of the annular protrusion on bottle cap lower surface and the inner surface clamping of bottleneck, outside annular protrusion, the bottle cap of brace offside is provided with the card be connected on bottleneck outer surface, collect in seed collection bottle and have Seeds of Dendrobium Candidum, bottle cap covers after on bottleneck, the outer surface of bottle cap and bottleneck junction is provided with ventilative sealed membrane, the jack of receiving flask dispenser is laid seed collection bottle,
Described method for preserving is completed by following steps:
(1) disinfecting utensils: put into overall disinfection after cup together with tweezers, scissors after seed collection bottle is opened bottle cap, put on superclean bench for subsequent use after sterilization;
(2) capsule sterilization: by full crackless dendrobium candidum capsule, alcohol-pickled 1 minute that is placed on 70% ~ 75% on superclean bench with the dust that sterile water carefully rinses on capsule, then sterilize 6 ~ 10 minutes with the mercuric chloride of 1g/L, finally use aseptic water washing 3 ~ 5 times, blot the moisture on capsule with aseptic filter paper;
(3) collection of seed: the base portion capsule disinfected being clamped in superclean bench capsule with tweezers, cut off with the top of scissors by capsule, the bottleneck of seed collection bottle is placed on bottle holder upward, the clip of capsule aims at the bottleneck of seed collection bottle down, beat the tweezers clamping capsule gently, seed is all poured in seed collection bottle, after the bottle cap of seed collection bottle is built, sealed with sealed membrane;
(4) stored refrigerated: the jack that the receiving flask dispenser of depository dish put into by the seed collection bottle that Seeds of Dendrobium Candidum is housed that sealing step (3) completed is good covers lid, moves into refrigeration in refrigerating chamber;
Wherein, described sealed membrane is PM-996 sealed membrane or INVENTIONPaper filter film or PTFE bacteria filters;
Described seed collection bottle polypropylene material is made;
Described refrigerating chamber is refrigerator, and the temperature of described refrigeration is 2-10 DEG C.
2. method for preserving according to claim 1, is characterized in that: described Seeds of Dendrobium Candidum is the corpuscular seed after removing capsule shell.
3. method for preserving according to claim 1, it is characterized in that: the inner surface of the opening of described box body is provided with a ring annular groove, described receiving flask dispenser is installed on described cannelure, the upper surface of receiving flask dispenser stretches out the upper surface of box opening, and the downside of described lid is fastened on the upper surface of box opening and stretches out on the lateral surface of receiving flask dispenser of box opening upper surface.
4. method for preserving according to claim 1, is characterized in that: the temperature of described refrigeration is 3 DEG C or 4 DEG C or 5 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410150279.1A CN103918418B (en) | 2014-04-15 | 2014-04-15 | The storage device of Seeds of Dendrobium Candidum and method for preserving thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410150279.1A CN103918418B (en) | 2014-04-15 | 2014-04-15 | The storage device of Seeds of Dendrobium Candidum and method for preserving thereof |
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| CN105875138A (en) * | 2015-04-29 | 2016-08-24 | 广西桂平市仙宝园铁皮石斛有限责任公司 | Method for breeding direct seeding seedlings through natural seeding of dendrobe seeds |
| CN105850282A (en) * | 2016-04-29 | 2016-08-17 | 巩庆飞 | Domestic plant seed germination machine |
| CN106167124A (en) * | 2016-07-18 | 2016-11-30 | 中国科学院昆明植物研究所 | A kind of freezer the storage of seeds box and manufacture method thereof |
| CN106717266B (en) * | 2016-12-09 | 2021-07-23 | 浙江大学 | Long-term preservation method of Dendrobium officinale capsules/seeds |
| CN110651559A (en) * | 2019-11-07 | 2020-01-07 | 安徽祥枫生物科技有限公司 | Preservation method of dendrobium huoshanense capsules for tissue culture seedling |
| CN112913386B (en) * | 2021-01-30 | 2023-06-16 | 濮阳市农业科学院 | Quick-cleaning wide-width precision wheat seeder |
| CN113039945B (en) * | 2021-03-31 | 2022-01-28 | 淮北市三丰肥业有限公司 | Seed storage equipment for agricultural |
| CN114476330B (en) * | 2022-02-28 | 2024-02-27 | 中国科学院东北地理与农业生态研究所 | Soil storage and freeze-drying sample bottle for microorganism detection and detection method thereof |
| CN114847275A (en) * | 2022-06-13 | 2022-08-05 | 贵州省亚热带作物研究所 | Long-term preservation method for dendrobium officinale seeds |
| CN115777537B (en) * | 2022-12-05 | 2024-08-23 | 黔西南民族职业技术学院 | Processing apparatus of plant capsule when tissue culture |
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| JP4176193B2 (en) * | 1998-05-26 | 2008-11-05 | 日本植生株式会社 | Preservation method of wet cryopreservation seed and water supply agent used therefor |
| EP2203363B1 (en) * | 2007-09-26 | 2014-04-02 | Pioneer-Hi-Bred International, Inc. | Apparatus and method to package articles for storage and identification |
| CN101278614A (en) * | 2008-05-30 | 2008-10-08 | 中国热带农业科学院橡胶研究所 | Low-temperature storage method for rubber tree seeds |
| CN202315975U (en) * | 2011-11-25 | 2012-07-11 | 成都康华生物制品有限公司 | Test tube stand box with refrigeration function |
| CN202407634U (en) * | 2011-12-30 | 2012-09-05 | 黄如玉 | Container cap |
| CN102893865B (en) * | 2012-09-20 | 2013-11-27 | 四川农业大学 | Preparation and storage method of dendrobium officinale artificial seeds |
| CN203088500U (en) * | 2012-12-19 | 2013-07-31 | 南京南汽专用车有限公司 | Convenient water cup |
| CN203788757U (en) * | 2014-04-15 | 2014-08-27 | 高辉 | Preserving device for dendrobium officinale seeds |
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