CN103878038B - A universal microfluidic chip fixture - Google Patents

Abstract

A general-purpose microfluidic chip fixture design, which is composed of fixture B plate, chip, polyvinyl fluoride blade ring, screw, fixture A plate, connecting screw, sleeve, glass capillary, etc.; the advantage of the present invention is that the chip adopts "V"-shaped hole, the glass capillary is pressed tightly by the connecting screw through the polyvinyl fluoride blade ring, the sealing performance is good, the operation is convenient, and the standard thread of 3/16"N=32 is convenient and economical; the two sides of the fixture A plate are cut , chip loading and unloading is convenient; there are 12 on the fixture B board Observation hole, easy to observe the installation status.

Description

A universal microfluidic chip fixture

Technical field:

The invention relates to the application technology of a microfluidic chip, and in particular provides a connection interface specially used for externally connecting a fluid to the microfluidic chip.

Background technique:

Micro Total Analysis Systems (μ-TAS) is a new interdisciplinary field, and its ultimate goal is to maximize the transfer of the functions of the analytical laboratory to portable analysis through the miniaturization and integration of chemical equipment. In the equipment (such as various chips), the "miniaturization" of the analysis equipment is realized, so the micro-analysis system is also popularly called "Lab-on-a-chip". According to the chip structure and working mechanism It can be divided into two categories: Microarray chip (Microarray chip), also known as "biological chip", and microfluidic chip (Microfluidic chip). The former is quite mature and has been deeply industrialized abroad; the latter is mainly Based on analytical chemistry and biochemistry, micro-electromechanical processing technology (MEMS) is used to process a 10-100 micron micro-channel network on the surface of silicon, glass, quartz, and plastic, and use electroosmotic flow and electrophoretic flow or directly use an external pump as The driving force controls the flow direction and rate of the fluid in the microchannel network, so as to realize the steps of sampling, diluting, adding reagents, enriching, extracting, mixing, reacting, separating, and detecting the target analyte. It is a very important part to realize the connection between the external fluid and the microfluidic chip. There must be an interface in the middle. At present, there are literature reports or the company’s publicity that the pressure resistance of the interface is not good (mainly the sealing method and material, mostly normal pressure , rarely more than 20KG), the operation is inconvenient. It hinders the application and development of microfluidic chip work.

Invention content:

The object of the present invention is to provide a connection interface specially used for external fluid of the microfluidic chip: the combined fixture includes fixture B plate, chip, polyvinyl fluoride blade ring, fixture A plate, connecting screw, sleeve, glass capillary;

The chip is a rectangular flat chip, with one or more liquid storage tanks on the left and right edges, and the middle part of the chip is connected to at least one left liquid storage tank and at least one right liquid storage tank. the microchannel;

Fixture B plate is a flat plate with a rectangular through hole in the middle of the plate.

Fixture A plate is a flat plate, with a rectangular through hole in the middle of the plate, and strip-shaped bosses are respectively set on the left and right sides of the lower surface of fixture A plate, and the long strip-shaped bosses on fixture A plate face fixture B plate On one side, the jig A board is fixed above the jig B board, and a slot for placing chips is formed between the jig A board and the jig B board; the chip is inserted into the slot;

The projection of the rectangular through hole between the fixture A board and the fixture B board on the slot is located in the middle of the slot, and the size is smaller than the size of the slot;

On the fixture A plate, there are through holes with internal threads corresponding to the liquid storage tanks on the left and right sides of the chip. , the front end of the connecting screw leans against the inlet or outlet of the liquid storage tank, the connecting screw is provided with an axial through hole, a sleeve is inserted in the axial through hole, a glass capillary is inserted in the sleeve, and the glass capillary extends into the storage tank. The inlet or outlet of the liquid tank; a polyvinyl fluoride ring is set on the outside of the front sleeve of the connecting screw,

The glass capillary and the sleeve are fixed on the connecting screw by a polyvinyl fluoride ring, and at the same time, the connection screw is sealed with the inlet or outlet of the liquid storage tank by a polyvinyl fluoride ring.

Compared with conventional microfluidic chip fixtures, the advantages of the present invention are:

1. The axial section of the upper half of the inlet or outlet of the liquid storage tank on the chip is inverted trapezoidal, and the axial section of the lower half is rectangular. Use the connecting screw to press the sleeve and the glass capillary tightly through the polyvinyl fluoride ring. , Good sealing performance, high pressure resistance (up to 70KG), easy operation, saving loading and unloading time.

2. The fixture adopts standard 3/16”N=32 thread, the accessories are easy to use, the selection range is wide, and it is economical and reliable.

3. Cut on both sides of the fixture A plate, so that the chip can be easily loaded and unloaded, and the operation is flexible.

4. There are 12 on the fixture B board The observation hole is convenient for observing whether the connecting screw is pressed tightly.

Description of drawings:

Fig. 1 Schematic diagram of a combined state of the universal microfluidic chip fixture.

Figure 2 Schematic diagram of the plane and section of plate A of the universal microfluidic chip fixture.

Detailed ways:

Connecting screw 6 is a PEEK connector (product model: Upchurch company, Ftg NT, PEEKHex-Head, 1/16),

As shown in Figure 1, a combined state of the general-purpose microfluidic chip fixture is shown. The combined fixture of the present invention consists of a fixture B plate, a chip, a polyvinyl fluoride blade ring, M3 screws, a fixture A plate, connecting screws, sleeves, Combination of glass capillary, etc.;

The axial cross-section of the upper half of the inlet or outlet of the liquid storage tank on the chip is an inverted trapezoidal shape, and the axial cross-section of the lower half is rectangular. There are 10 M3 fixing screw holes and 12 Observation holes, there are 10 holes on fixture A plate corresponding to the M3 fixing screw holes on fixture B plate 12 holes of 3/16"N=32 and 12 on fixture B plate Observe the screw hole corresponding to the hole.

The fixture A plate is an inverted groove with a rectangular groove on the bottom surface, so that the chip can move freely left and right in the fixture. The surface height of the fixture A plate and the surface of the fixture B plate is 2-3 times the height of the chip.

The 10 corresponding holes on the jig B board and the jig A board are respectively fixed with M3 screws and combined into a whole.

Fasten fixture B board 1 and fixture A board 5, and fix and tighten with M3 screw 4; insert chip 2 through the cutouts on both sides of fixture A board, and insert it into the fixture; align the inverted trapezoidal hole of the chip with the 3 /16"N=32 screw holes, use the connecting screw 6 to pass through the polyvinyl fluoride blade ring 3, and press the sleeve 7 and the glass capillary tightly 8.

Claims (6)

1. A universal microfluidic chip fixture, characterized in that: the combination fixture includes fixture B plate (1), chip (2), polyvinyl fluoride blade ring (3), fixture A plate (5), connecting screw (6), casing (7), glass capillary (8); The chip (2) is a rectangular flat chip, with one or more liquid storage tanks respectively arranged on the left and right sides of the chip, and the middle part of the chip is provided with at least one left liquid storage tank and at least one right storage tank respectively. Microchannels connected to the tank; Fixture B plate (1) is a flat plate with a rectangular through hole in the middle of the plate. Fixture A plate (5) is a flat plate with a rectangular through hole in the middle of the plate, and strip-shaped bosses are respectively provided on the left and right sides of the lower surface of the fixture A plate. The long strip-shaped bosses on the fixture A plate face On one side of the fixture B plate, the fixture A plate is fixed above the fixture B plate, and a slot with two ends open for placing the chip (2) is formed between the fixture A plate and the fixture B plate; the chip (2) is inserted into the slot ; The projection of the rectangular through hole between the fixture A board and the fixture B board on the slot is located in the middle of the slot, and the size is smaller than the size of the slot; There are through holes with internal threads corresponding to the liquid storage tanks on the left and right sides of the chip (2) on the fixture A plate, and the through holes correspond to the liquid storage tanks on the chip (2) one by one; The connecting screw (6) with external thread, the front end of the connecting screw (6) leans against the inlet or outlet of the liquid storage tank, the connecting screw (6) is provided with an axial through hole, and a sleeve is inserted in the axial through hole A glass capillary (8) is inserted into the tube (7) and sleeve (7), and the glass capillary (8) extends into the inlet or outlet of the liquid storage tank; it is connected to the outside of the front sleeve (7) of the screw (6) Set with polyvinyl fluoride edge ring (3), Fix the glass capillary (8) and sleeve (7) on the connecting screw (6) through the polyvinyl fluoride ring (3), and at the same time connect the connecting screw (6) to the liquid storage tank through the polyvinyl fluoride ring (3). Seal between the inlet or outlet. 2. The microfluidic chip fixture according to claim 1, characterized in that: the fixture A plate and the fixture B plate are fixedly connected by screws (4). 3. According to the microfluidic chip fixture according to claim 1, it is characterized in that: on the fixture B plate (1), an observation hole corresponding to the through hole with internal thread on the fixture A plate and the chip (2) is provided. 4. The microfluidic chip fixture according to claim 1, characterized in that: the axial cross-section of the upper half of the inlet or outlet of the liquid storage tank on the chip is an inverted trapezoidal shape, and the axial cross-section of the lower half is rectangular. 5. According to the microfluidic chip fixture according to claim 1, it is characterized in that: there are 10 M3 fixing screw holes and 12 fixing screw holes on the fixture B plate. Observation holes, there are 10 holes on fixture A plate corresponding to the M3 fixing screw holes on fixture B plate 12 holes for 3/16"N=32 and 12 on fixture B plate The screw hole corresponding to the observation hole; Fixture B board and fixture A board are fixed with M3 screws and combined into a whole; The fixture A plate is an inverted groove with a rectangular groove on the bottom surface, so that the chip can move freely left and right in the fixture. The height of the bottom of the fixture A plate and the surface of the fixture B plate is 2-3 times the thickness of the chip. 6. The microfluidic chip fixture according to claim 1, characterized in that: the shape and size of the chip (2) and the slot correspond to each other; the height of the slot is 2-3 times the thickness of the chip.