CN103875846A - Bagged antrodia camphorata strain and pseudostellaria heterophylla tea and preparation method thereof - Google Patents

Abstract

The present invention relates to a tea bag fermented with fungi and ginseng and a preparation method thereof, specifically disclosing an application of Antrodia camphorata in fermenting and preparing tea bag fermented with fungus and ginseng and a method for preparing tea bag fermented with fungus and ginseng by fermenting heterophylla from Antrodia camphorata. The above-mentioned Antrodia camphorata strains were purchased from ATCC, No. 200183. The present invention uses Antrodia camphorata as the fermentation strain, cultures and ferments Pseudostellariae Radix Pseudostellariae as the main substrate raw material, and after post-processing and bagging, obtains ginseng fermented teabag, and the crude polysaccharides, total triterpenes and total amino acids in the tea soup The content is higher than that of the control. The soup is clear and transparent, with a mellow taste and the unique fruity aroma of Antrodia camphorata.

Description

A kind of ginseng fermented tea bag and preparation method thereof

technical field

The invention relates to a fungus ginseng fermented tea bag and a preparation method thereof, in particular to a fermented tea containing antrodia camphorata and heterophylla and a processing technology thereof, belonging to the field of tea substitutes. the

Background technique

Pseudostellaria heterophylla (Miq.) Pax ex Pax et Hoffm.] is the dry tuber root of Pseud ostellaria heterophylla (Miq.) Pax ex Pax et Hoffm. , the effect is similar to that of ginseng, but the strength is weaker, and the medicinal power is mild. It is good at clearing and nourishing, suitable for all ages. Studies have shown that Pseudostellariae Pseudostellariae is rich in nutrients and functional components such as sugars, cyclic peptides, amino acids, saponins, and trace elements (Qin Minjian, Yu Yongbang, Huang Wenzhe, etc. Quality analysis of Pseudostellariae Pseudostellariae from different origins. Modern Chinese Medicine Research and Practice, 2005, 19(5): 29-32; Lin Mao, Zheng Jiong, Yang Lin, et al. Analysis of chemical components in Radix Pseudostellariae from different origins. Food Science, 2012, 33(2): 204-207), has anti-fatigue, Anti-oxidation, anti-stress, hypoglycemic, and immune-enhancing effects (Wong C K, Leung K N, Fung K P. The immunostimulating activities of anti-tumor polysaccharides from Pseudostellaria heterophylla. Immunopharmacology, 1994, 28(1): 47 -54; NG T B, LIU F, WANG H X. The antioxldant effects of aqueous and organic extracts of Panax quinquefolium, Panax notoginseng, Codonopsis pilosula, Pseudostella riaheterophylla and Glehnia littoralis. Ethnopharmacol, 8(234-8) ; Wu Chaofeng, Lin Yanquan. The research progress of the medicinal plant Pseudostellariae Pseudostellariae. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2004, 33(4): 426-430), which has extremely high edible and medicinal value, and is a kind of beneficial It is a precious and nutritious tonic product with the functions of strengthening the spleen, promoting body fluid and moistening the lungs. the

Before the 1970s, Pseudostellariae Pseudostellaria was mainly wild, and it was distributed in East my country, Central China, North China, and Northwest China; after expanding cultivation, it is now planted and cultivated on a large scale in Fujian, Shandong, Jiangsu, and Anhui. The resources are very rich, and carrying out research on edible development of Radix Pseudostellariae can stimulate consumer demand and improve the full utilization of its resources, which is of great significance for the development of regional economy and the transformation of economic development mode. Pseudostellariae Radix Pseudostellariae is traditionally consumed as dietary raw material for making soup or extracting active ingredients into tablets and oral liquids. The tonic and health-care tea products that are the main raw materials and formulas are constantly being developed, and have great application prospects because they are easy to carry and eat. the

Chinese patent CN103404676A discloses a preparation method of heterophylla heterophylla tea, which consists of only heterophylla heterophylla, and the method includes raw material selection, grade screening, cleaning, drying, roasting and processing, crushing, sealing and packaging. the

Pseudostellaria Pseudostellariae White Tea is composed of Zherong Pseudostellaria Pseudostellariae and Fuding White Tea according to a certain ratio, which not only maintains the anti-radiation properties of white tea, but also increases the health care functions of Pseudostellaria Pseudostellariae to invigorate Qi and spleen, nourish yin and promote body fluid (Cai Binxin, Chen Aiping, Lin Jinke, et al. . Radiation protection effect of heterophylla heterophylla white tea. Journal of Ningde Normal University (Natural Science Edition), 2013, 25(1): 21-27.). the

The prescription of Shenmai tea is 9g of heterophylla, 15g of floating wheat, and 20 pieces of red dates, which have the effect of invigorating qi and promoting blood (Zhang Jie, Yu Huasheng, You Yongji. Edible and medicinal value of zherong heterophylla. Strait Pharmacy, 2011 , 23(5): 48-50). the

Chinese patent CN10208492A discloses a preparation method of honeysuckle heterophylla heterophylla tea. The preparation method includes raw material selection and ingredients. Take 6g of honeysuckle and 15g of heterophylla heterophylla, mix them after crushing, and obtain honeysuckle heterophylla heterophylla tea, which has the functions of invigorating qi and clearing away heat. . the

These developed Pseudostellariae Pseudostellaria tea products are all conventionally crushed raw materials, and are simply mixed with Pseudostellariae Pseudostellariae and other foods (or medicines). However, only by crushing to increase the precipitation of functional components of Pseudostellariae Pseudostellariae tea, the effect is limited, and too fine pulverization will easily affect the clarity of tea soup; in addition, Pseudostellariae Pseudostellariae itself contains methyl mercaptan, dimethyl sulfide, small molecular furans and furfural, etc. Compounds that emit a specific odor similar to "666" (Qian Wei, Han Le, Liu Xunhong, etc. HSGC-MS analysis and research on the specific odor components of Pseudostellaria Radix Pseudostellariae. Modern Chinese Medicine Research and Practice, 2010, 24(5 ): 25-27), affect the taste, simple mixing can not significantly improve the original special smell of heterophylla. the

Antrodia cintrodia is a rare edible fungus unique to Taiwan. It has the functions of calming the nerves, mildly reducing stagnation, detoxifying and reducing swelling, anti-oxidation, immune enhancement, liver protection, and anti-tumor (Li-Shian Shi, Chih-Hua Chao, De-Yang Shen, et al. Biologically active constituents from the fruiting body of Taiwan anofungus camphorates. Bioorganic & Medicinal Chemistry, 2011, 19: 677-683), which can decompose and use substrates such as lignin and cellulose for growth. the

Antrodia camphorata was fermented and cultivated by using heterophylla pseudostellariae raw materials to obtain fermented teabags with fungus ginseng. The content of functional components in the fermented tea soup has been significantly improved; at the same time, the quality of the tea has been significantly improved. The tea soup has a good color and a round taste. accept. the

the

SUMMARY OF THE INVENTION The purpose of the present invention is to provide an Antrodia camphorata ( Antrodia camphorata, purchased from ATCC, preservation number 200183), in terms of fermenting and preparing Pseudostellaria heterophylla tea, promoting the separation of functional components of Pseudophylloides heterophylla tea products, and improving the smell of Pseudostellaria heterophylla tea products Applications.

The object of the present invention is also to provide a method for preparing ginseng fermented tea bag by fermenting Radix Pseudostellariae from Antrodia camphorata. the

A kind of method that Antrodia camphorata fermentes heterophylla heterophylla prepares ginseng fermented tea in bag comprises the following steps:

(1) Raw material processing and proportioning:

Wash the Radix Pseudostellariae, spread out and dry in the sun, crush and pass through a 20-mesh sieve; prepare the material by mixing Pseudostellariae powder, soybean powder, corn flour, and edible gypsum, sterilize it in a sterilizer, and cool it for later use;

(2) Strain preparation:

Inoculate the slant strains of Antrodia camphorata (about 5mm×5mm in size) into potato dextrose agar (PDA), and cultivate them in a constant temperature and humidity incubator to obtain the plate strains of Antrodia camphorata;

(3) Inoculation fermentation:

In the ultra-clean workbench, divide the strains of Antrodia camphorata plate into small pieces of about 2.5mm×2.5mm, inoculate them into the sterilized fermentation raw materials, and fully mix them with the raw materials; place them in a constant temperature and humidity incubator for purification nourish;

(4) Post-processing and packaging of fermented tea:

After the fermentation is over, take out the fermented product and dry it, crush the agglomerates and mix well, put them into food-grade filter paper tea bags (2 g/bag) and seal them to make ginseng fermented teabags.

Wherein the ratio of raw materials described in step (1) is that every 100g of material contains 85g-97g of Radix Pseudostellaria powder, 1g-10g of soybean powder, 1g-10g of corn flour, and 1g-5g of edible gypsum; The water content of the material is controlled at 40 g-65 g per 100 g of water content. the

The culture condition in step (2) is 27°C for 12 days; the PDA medium includes the following components: 200 g of potatoes, 20 g of glucose, 20 g of agar, dilute water to 1000 mL, and natural pH. the

Wherein the pure culture condition described in step (3) is 25°C-32°C for 15 d-30 d. the

Wherein the step (4) is dried until the moisture content per 100 g of the material is less than 5 g; the ginseng fermented tea bag is 2 g/bag. the

Beneficial effect:

The existing reported or published Pseudostellariae tea preparation methods are conventional pulverization of raw materials and simple mixing of Pseudostellariae and other foods (or medicines). There is no report on the related results of fermenting Pseudostellariae Pseudostellariae to prepare fermented tea. Pseudostellaria heterophylla itself contains compounds such as methyl mercaptan, dimethyl sulfide, small molecule furans and furfural, which emit a peculiar smell like "666", which affects the taste when taken, and it is easy to mix it with other ingredients The treatment could not significantly improve the original special smell of Pseudostellariae Radix.

The present invention ferments the heterophylla to prepare fermented tea, the quality of the tea is significantly improved, the tea soup presents a good color, the taste is mellow, and the unique fruity aroma of Antrodia antrodia improves the original special smell of the heterophylla, making it easier to accept ; and the crude polysaccharides of fermented tea, total triterpenes and total amino acids and other functional components are more likely to be released due to the degradation of Antrodia camphorata on the cellulose and lignin of Radix Pseudostellariae, and the content in the tea soup is significantly improved compared with the control. the

Detailed ways

The present invention will be further described in conjunction with embodiment now. the

Embodiment 1: the preparation of raw material:

(1) Preparation of PDA medium

Potato 200 g, glucose 20 g, agar 20 g, water to 1000 mL, natural pH. 115 ℃, 0.1 MPa autoclave for 20 min.

(2) Activation of Antrodia camphorata strains

The strains of Antrodia camphorate ( Antrodia camphorate, 200183) stored in the refrigerator were inoculated on the PDA slant medium in a clean bench, and cultured in a constant temperature and humidity incubator at 27 °C for 10 days to obtain the slant activated strains.

(3) Preparation of Antrodia Antrodia Plate Strains

The activated strains of Antrodia slant (about 5 mm × 5 mm in size) were inoculated on the center of the PDA plate, and cultured in a constant temperature and humidity incubator at 27 °C for 12 days to obtain the strains of Antrodia camphorata plate.

(4) Preparation of Pseudostellariae Powder

Selection of materials: Select Pseudostellariae Radix Pseudostellariae with no pests and diseases, no mildew, and uniform size, and set aside.

Cleaning and drying: Wash the selected Radix Pseudostellariae with clean water to remove the dirt and floating ash on the surface, spread the cleaned Radix Pseudostellaria evenly in the bamboo plaques, pay attention to ventilation, and let it dry naturally. the

Crush and sieve: Grind the naturally dried Pseudostellariae Pseudostellariae with a pulverizer, pass through a 20-mesh sieve to obtain Pseudostellariae Pseudostellaria powder, and set aside. the

Example: 2:

Take 85 g of heterophylla ginseng powder, 10 g of soybean powder, 10 g of corn flour, and 5 g of edible gypsum for raw material preparation, control the water content to 40 g/100 g of material, sterilize in a sterilizing pot, and cool. Use an inoculation shovel to inoculate the Antrodia camphorata plate strain in Example 1 into the sterilized fermentation raw material in the ultra-clean workbench, and fully mix it with the raw material. The inoculated materials were placed in a 25°C constant temperature and humidity incubator for pure culture for 15 days. After the fermentation is over, take out the fermented product and dry it until the moisture content is less than 5 g per 100 g of material, crush the agglomerates and mix well, put them into food-grade filter paper tea bags (2 g/bag) and seal them to make bacteria Ginseng fermented teabag.

Example 3:

Take 95 g of Pseudostellariae powder, 3 g of soybean powder, 1 g of corn flour, and 1 g of edible gypsum for raw material preparation, control the water content to 55 g/100 g of material, sterilize in a sterilizing pot, and cool. Use an inoculation shovel to inoculate the Antrodia camphorata plate strain in Example 1 into the sterilized fermentation raw material in the ultra-clean workbench, and fully mix it with the raw material. The inoculated materials were placed in a 25°C constant temperature and humidity incubator for pure culture for 20 days. After the fermentation is over, take out the fermented product and dry it until the moisture content is less than 5 g per 100 g of material, crush the agglomerates and mix well, put them into food-grade filter paper tea bags (2 g/bag) and seal them to make bacteria Ginseng fermented teabag.

Example 4:

Take 90 g of Pseudostellariae powder, 5 g of soybean powder, 3 g of corn flour, and 2 g of edible gypsum for raw material preparation, control the water content to 45 g/100 g of material, sterilize in a sterilizing pot, and cool. Use an inoculation shovel to inoculate the Antrodia camphorata plate strain in Example 1 into the sterilized fermentation raw material in the ultra-clean workbench, and fully mix it with the raw material. The inoculated materials were placed in a 32°C constant temperature and humidity incubator for pure culture for 18 days. After the fermentation is over, take out the fermented product and dry it until the moisture content is less than 5 g / 100 g of material, crush the agglomerates and mix them well, put them into food-grade filter paper tea bags (2 g / bag) and seal them to make bacteria Ginseng fermented teabag.

Example 5:

Take 97 g of Pseudostellariae powder, 1 g of soybean powder, 1 g of corn flour, and 1 g of edible gypsum for raw material preparation, control the water content to 65 g/100 g of material, sterilize in a sterilizing pot, and cool. Use an inoculation shovel to inoculate the Antrodia camphorata plate strain in Example 1 into the sterilized fermentation raw material in the ultra-clean workbench, and fully mix it with the raw material. The inoculated materials were placed in a 27°C constant temperature and humidity incubator for pure culture for 30 days. After the fermentation is over, take out the fermented product and dry it until the moisture content is less than 5 g / 100 g of material, crush the agglomerates and mix them well, put them into food-grade filter paper tea bags (2 g / bag) and seal them to make bacteria Ginseng fermented teabag.

Brew the teabag prepared in Example 2-5 with tea water ratio of 1:50, and brew it with boiling water for 5 minutes, and evaluate the tea soup together with the corresponding uninoculated control, and the results are recorded as follows:

Table 1 Quality of fermented tea soup and content of functional components

Claims (6)

1. a ginseng fermented tea bag is characterized in that, utilizes heterophylla heterophylla raw material to carry out fermentation preparation to Antrodia camphorata. 2. a preparation method for bacterial ginseng fermented tea in bag, is characterized in that, carries out according to the following steps: (1) Raw material processing and proportioning: Wash the Radix Pseudostellariae, spread out and dry in the sun, crush and pass through a 20-mesh sieve; prepare the material by mixing Pseudostellariae powder, soybean powder, corn flour, and edible gypsum, sterilize it in a sterilizer, and cool it for later use; (2) Strain preparation:   Inoculate the slanted strains of Antrodia Antrodia into PDA medium and cultivate them in a constant temperature and humidity incubator to obtain the plate strains of Antrodia Antrodia; (3) Inoculation fermentation: In the ultra-clean workbench, divide the strains of Antrodia cinnamomea into small pieces, inoculate them into the sterilized fermentation raw materials, and fully mix them with the raw materials; place them in a constant temperature and humidity incubator for pure cultivation; (4) Post-processing and packaging of fermented tea: After the fermentation is over, take out the fermented product and dry it, crush the agglomerates and mix them well, put them into food-grade filter paper tea bags and seal them to make ginseng fermented teabags. 3. The preparation method of a kind of ginseng fermented teabag according to claim 2, characterized in that, the ratio of raw materials described in step (1) is that every 100g of material contains 85 g-97 g of Pseudostellaria ginseng powder, Soybean powder 1 g-10 g, corn flour 1 g-10 g, edible gypsum 1 g-5 g; the water content of the materials is controlled at 40 g-65 g per 100 g. 4. The preparation method of a kind of ginseng fermented teabag according to claim 2, characterized in that, the culture condition in step (2) is 27°C for 12 days; the PDA medium includes the following components : Potato 200 g, glucose 20 g, agar 20 g, water to 1000 mL, natural pH. 5. The method for preparing teabags fermented with fungus ginseng according to claim 2, characterized in that the pure culture condition in step (3) is 25°C-32°C for 15 d-30 d. 6. The preparation method of a kind of ginseng fermented teabag according to claim 2, characterized in that, in the step (4), drying is carried out until the moisture content of each 100 g material is less than 5 g; the ginseng fermentation Teabags are 2 g/bag.