CN103852369B - Diluting device and method - Google Patents
Diluting device and method Download PDFInfo
- Publication number
- CN103852369B CN103852369B CN201410112034.XA CN201410112034A CN103852369B CN 103852369 B CN103852369 B CN 103852369B CN 201410112034 A CN201410112034 A CN 201410112034A CN 103852369 B CN103852369 B CN 103852369B
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- Prior art keywords
- peristaltic pump
- pump
- bottle
- dilution
- solvent
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- 238000000034 method Methods 0.000 title claims description 8
- 238000007865 diluting Methods 0.000 title abstract 7
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 94
- 238000010790 dilution Methods 0.000 claims abstract description 41
- 239000012895 dilution Substances 0.000 claims abstract description 41
- 239000002904 solvent Substances 0.000 claims abstract description 33
- 239000011550 stock solution Substances 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 239000000243 solution Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000003085 diluting agent Substances 0.000 claims description 12
- 238000003113 dilution method Methods 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims 1
- 239000012530 fluid Substances 0.000 description 8
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D11/00—Control of flow ratio
- G05D11/02—Controlling ratio of two or more flows of fluid or fluent material
- G05D11/13—Controlling ratio of two or more flows of fluid or fluent material characterised by the use of electric means
- G05D11/131—Controlling ratio of two or more flows of fluid or fluent material characterised by the use of electric means by measuring the values related to the quantity of the individual components
- G05D11/132—Controlling ratio of two or more flows of fluid or fluent material characterised by the use of electric means by measuring the values related to the quantity of the individual components by controlling the flow of the individual components
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Accessories For Mixers (AREA)
- Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a diluting device. The diluting device comprises a solvent bottle, a stock solution bottle, a diluting bottle, a first peristaltic pump, a second peristaltic pump, pump pipes and a control device, wherein a liquid inlet of the first peristaltic pump is communicated with the solvent bottle through the pump pipes, and a liquid outlet of the first peristaltic pump is communicated with the diluting bottle through the pump pipes; an inlet of the second peristaltic pump is communicated with the stock solution bottle through the pump pipes, and an outlet of the second peristaltic pump is communicated with the diluting bottle through the pump pipes; the first peristaltic pump and the second peristaltic pump are electrically connected with the control output end of the control device respectively, and a temperature sensor for being immersed into a solution to detect the temperature of the solution is further arranged on the control device. The diluting device is high in dilution accuracy and low in facility cost and is simple and convenient to operate.
Description
Technical field
The present invention relates to a kind of diluent facilities, more particularly to a kind of diluent facilities and method of peristaltic pump type.
Background technology
Accurate dilutions refer to the solvent that certain volume is added in certain volume stock solution, obtain the mistake of an accurate target liquid
Journey.The method of some employing manual dilutions：Accurately measuring device is accurate by stock solution using pipet, volumetric flask etc. for speciality chemical reviewer
Target liquid is really diluted to, this process is high to the operation of laboratorian chemical examination level requirement, operating procedure is more, elapsed time length, need people
Work is calculated, and the accuracy and precision that frequently can lead to dilute result is not so good as people's will；At present weight is mainly used on the market
Dilution method：Profit weighs with scale and shows the weight of the weight and addition solvent of stock solution, after setting extension rate, is diluted.
This is a kind of gravimetric method, but because the density of stock solution and solvent is affected by concentration and temperature, its repeatability is 1%, nothing
The higher precision of normal direction breaks through.
The content of the invention
The technical problem to be solved is：A kind of diluent facilities and method are provided, not only dilution precision is higher,
And it is simple, convenient, equipment cost is low.
To solve above-mentioned technical problem, the present invention proposes a kind of diluent facilities, including solvent bottle, stock solution bottle, dilution bottle,
First peristaltic pump, the second peristaltic pump, pump line and control device, the liquid inlet of first peristaltic pump is by pump line and solvent bottle
Connection, the liquid outlet of the first peristaltic pump is connected by pump line with dilution bottle；The entrance of second peristaltic pump is by pump line and original
Liquid bottle is connected, and the outlet of the second peristaltic pump is connected by pump line with dilution bottle；First peristaltic pump, the second peristaltic pump respectively with
The control output end electrical connection of control device, is used to detect solution temperature in being additionally provided with the control device for immersing solution
Temperature sensor.
Further, also including support, the support is provided with chuck, and the pump line is consolidated with the end of dilution bottle connection end
It is scheduled on the chuck.
Further, drainage tube is provided with the dilution bottle, the upper end of the drainage tube is connected with pump line.
Further, the alarm device being connected with the control device is also included.
Further, the alarm device is loudspeaker either alarm lamp or vibrator.
To solve above-mentioned technical problem, the invention allows for a kind of dilution process, comprises the steps：
Step 2, formulates and calibrates the first peristaltic pump：Pure water is poured in solvent bottle, with temperature sensor solvent bottle is detected
The temperature of middle pure water, and the density p 1 of pure water at this temperature is recorded, then start the first wriggling pump work, record the first peristaltic pump
Duration of runs t1, by the weight of solvent m1 of the gentle peristaltic pump of measuring cup precise first in day output, calculate the first wriggling
The flow value q1=m1/ (ρ 1*t1) of pump；
Step 3, formulates and calibrates the second peristaltic pump：Pure water is poured in stock solution bottle, with temperature sensor stock solution bottle is detected
The temperature of middle pure water, and the density p 2 of pure water at this temperature is recorded, then start the second wriggling pump work, record the second peristaltic pump
Duration of runs t2, by the weight of solvent m2 of the gentle peristaltic pump of measuring cup precise second in day output, calculate the second wriggling
The flow value q2=m2/ (ρ 2*t2) of pump；
Step 4, dilution, according to original liquid concentration, target liquid concentration, target liq product, the first peristaltic pump flow value q1 with
And second peristaltic pump flow value q2, calculate the duration of runs T1 of the first peristaltic pump and duration of runs T2 of the second peristaltic pump；
Control device controls respectively the first wriggling pump work T1 times and the second wriggling pump work T2 times, realizes automatic and serial dilution
Purpose.
Further, before the step 2, also including step 1, control device controls respectively the first peristaltic pump and second
Wriggling pump work, allows solvent and stock solution full of whole pump line and does not produce bubble, and guarantees that whole pump line is hydraulically full.
Further, also including step 5, control device control alarm device warning reminding..
Above-mentioned technical proposal at least has the advantages that：The present invention obtains solvent bottle and stock solution by temperature sensor
The temperature information of pure water, is accurately calibrated to the flow of the first peristaltic pump, the second peristaltic pump in bottle, then in dilution work
In the first peristaltic pump, the working condition of the second peristaltic pump and operational factor are controlled by control device, because peristaltic pump is being pipetted
The repetition sexual deviation of 50ml solution is only ± 0.08%, better than the volume deviation of 50mlA level product volumetric flasks（±0.1%）, so dilute
Release precision higher, and can be performed continuously dilution operation, it is not only simple, convenient, and also equipment cost is low.
Description of the drawings
Fig. 1 is the structural representation of diluent facilities of the present invention.
Specific embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the application can phase
Mutually combination.The present invention is described further below in conjunction with the accompanying drawings.
As shown in figure 1, the diluent facilities of the embodiment of the present invention include that solvent bottle 1, stock solution bottle 2, dilution bottle 3, first are wriggled
Pump 4, the second peristaltic pump 5, pump line 6, control device 7, support 9, drainage tube 11 and alarm device, the first peristaltic pump 4 enters liquid
Mouth is connected by pump line 6 with solvent bottle 1, and the liquid outlet of the first peristaltic pump 4 is connected by pump line 6 with dilution bottle 3；Second peristaltic pump
5 entrance is connected by pump line 6 with stock solution bottle 2, and the outlet of the second peristaltic pump 5 is connected by pump line 6 with dilution bottle 3；First is compacted
Dynamic pump 4, the second peristaltic pump 5 are electrically connected respectively with the control output end of control device 7, are additionally provided with control device 7 for immersing
It is used to detect the temperature sensor 8 of solution temperature in solution.
Before dilution, need to calibrate the flow of the first peristaltic pump 4, the second peristaltic pump 5, first, to first
When peristaltic pump 4 is calibrated, pure water is poured in solvent bottle 1, with temperature sensor 8 temperature of pure water in solvent bottle 1 is detected, and
The density p 1 of pure water at this temperature is recorded, is then started the first peristaltic pump 4 and is worked, record the duration of runs t1 of the first peristaltic pump 4,
By the weight of solvent m1 of the gentle output of the first peristaltic pump of measuring cup precise 4 in day, the flow value q1 of the first peristaltic pump 4 is calculated
=m1/(ρ1*t1)；Secondly, when calibrating to the second peristaltic pump 5, pure water is poured in stock solution bottle 2, is examined with temperature sensor 8
The temperature of pure water in survey stock solution bottle 2, and the density p 2 of pure water at this temperature is recorded, then start the second peristaltic pump 5 and work, record
The duration of runs t2 of the second peristaltic pump 5, by the weight of solvent m2 of the gentle output of the second peristaltic pump of measuring cup precise 5 in day, meter
Calculate the flow value q2=m2/ (ρ 2*t2) of the second peristaltic pump 5.In dilution work, it is compacted that control device 7 can directly control first
The working condition and operational factor of the peristaltic pump 5 of dynamic pump 4 and second.
Support 9 is provided with chuck 10, and pump line 6 is fixed on chuck 10 with the end of the connection end of dilution bottle 3, such pump line 6
The bottle mouth position of dilution bottle 3 can be firmly fixed to, in order to avoid in dilution, it is to avoid pump line 6 when tube fluid flows out due to producing
Impulsive force and produce and rock, or even fluid spills outside dilution bottle 3, affects dilution precision.
Drainage tube 11 is arranged in dilution bottle 3, and the upper end of drainage tube 11 is connected with pump line 6, and such fluid can be slow
Drained in dilution bottle 3, it is to avoid fluid impact liquid level, spilt outside dilution bottle 3, impact dilution precision, and drainage tube 11
Length is about 5 centimeters, can easily remove dilution bottle.
Alarm device is connected with control device 7, and alarm device is loudspeaker, or alarm lamp or vibrator, so
After the completion of dilution, the control loudspeaker of control device 7 send alarm sound, remind staff's dilution end-of-job.
During work, the first peristaltic pump 4, the second peristaltic pump 5 are alternately extruded and released by the elastic delivery hose to pump
Put to pump fluid, the flow of fluid is depended on " pillow " that the rotating speed of pump head and the size of " pillow ", rotor every revolution are produced
The product of number this three parameters, " pillow " refers to " pillow " shape fluid that one section of pump line 6 between two transfer rollers is formed.
The present invention obtains the temperature information of pure water in solvent bottle 1 and stock solution bottle 2 by temperature sensor 8, wriggles to first
Pump 4, the flow of the second peristaltic pump 5 are accurately calibrated, and are then wriggled by the control of control device 7 first in dilution work
Pump 4, the working condition of the second peristaltic pump 5 and operational factor, because peristaltic pump is only in the repetition sexual deviation for pipetting 50ml solution
± 0.08%, better than the volume deviation of 50mlA level product volumetric flasks（±0.1%）, so dilution precision is higher, and can continuously enter
Row dilution operation, it is not only simple, convenient, and also equipment cost is low.
The dilution process of the embodiment of the present invention, comprises the steps：
Step 2, formulates and calibrates the first peristaltic pump 4：Pure water is poured in solvent bottle 1, with temperature sensor 8 solvent is detected
The temperature of pure water in bottle 1, and the density p 1 of pure water at this temperature is recorded, then starting the first peristaltic pump 4 and work, record first is compacted
The duration of runs t1 of dynamic pump 4, by the weight of solvent m1 of gentle the first peristaltic pump of measuring cup precise 4 in day output, calculates the
The flow value q1=m1/ (ρ 1*t1) of one peristaltic pump 4；
Step 3, formulates and calibrates the second peristaltic pump 5：Pure water is poured in stock solution bottle 2, with temperature sensor 8 stock solution is detected
The temperature of pure water in bottle 2, and the density p 2 of pure water at this temperature is recorded, then starting the second peristaltic pump 5 and work, record second is compacted
The duration of runs t2 of dynamic pump 5, by the weight of solvent m2 of gentle the second peristaltic pump of measuring cup precise 5 in day output, calculates the
The flow value q2=m2/ (ρ 2*t2) of two peristaltic pumps 5；
Step 4, dilution, according to original liquid concentration, target liquid concentration, target liq product, the first peristaltic pump 4 flow value q1 with
And second peristaltic pump 5 flow value q2, calculate the duration of runs T1 of the first peristaltic pump 4 and the duration of runs of the second peristaltic pump 5
T2；Control device controls respectively the work T1 times of the first peristaltic pump 4 and the work T2 times of the second peristaltic pump 5, realizes automatically and connects
The purpose of continuous dilution.
Used as another embodiment, before the step 2, also including step 1, rinse pipeline, control device 7 is distinguished
Control the first peristaltic pump 4 and the second peristaltic pump 5 works, allow solvent and stock solution full of whole pump line 6 and do not produce bubble, and guarantee
Whole pump line 6 is hydraulically full.Rinse pipeline can fully discharge bubble, impurity in pump line 6, will not pollute the fluid in pipe,
Precision is higher.
Used as another embodiment, also including step 4, the control alarm device warning reminding of control device 7 so can be with
Staff is notified in time, and dilution work is over, using more convenient.
The above is the specific embodiment of the present invention, it is noted that for those skilled in the art
For, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (3)
1. a kind of dilution process of diluent facilities, it is characterised in that：
The diluent facilities include solvent bottle, stock solution bottle, dilution bottle, the first peristaltic pump, the second peristaltic pump, pump line, control
Device and the alarm device being connected with control device, the liquid inlet of first peristaltic pump is connected by pump line with solvent bottle,
The liquid outlet of the first peristaltic pump is connected by pump line with dilution bottle；The liquid inlet of second peristaltic pump is by pump line and stock solution bottle
Connection, the liquid outlet of the second peristaltic pump is connected by pump line with dilution bottle；First peristaltic pump, the second peristaltic pump respectively with control
The control output end electrical connection of device processed, is additionally provided with immersion solution for detecting the temperature of temperature of liquid on the control device
Sensor, diluent facilities also include support, and the support is provided with chuck, and the pump line is fixed with the end of dilution bottle connection end
On the chuck；
Methods described includes：
Step 1, control device controls respectively the first peristaltic pump and the second wriggling pump work, allows solvent and stock solution to be full of whole pump line
And bubble is not produced, and guarantee that whole pump line is hydraulically full；
Step 2, it is intended that and calibrate the first peristaltic pump；Pure water is poured in solvent bottle, detects pure in solvent bottle with temperature sensor
The temperature of water, and the density p 1 of pure water at this temperature is recorded, then start the first wriggling pump work, record the fortune of the first peristaltic pump
Turn time t1, by the weight of solvent m1 of the gentle peristaltic pump of measuring cup precise first output in day, calculate the first peristaltic pump
Flow value q1=m1/ (ρ 1*t1)；
Step 3, it is intended that and calibrate the second peristaltic pump；Pure water is poured in solvent bottle, detects pure in stock solution bottle with temperature sensor
The temperature of water, and the density p 2 of pure water at this temperature is recorded, then start the second wriggling pump work, record the fortune of the second peristaltic pump
Turn time t2, by the weight of solvent m2 of the gentle peristaltic pump of measuring cup precise second output in day, calculate the second peristaltic pump
Flow value q2=m2/ (ρ 2*t2)；
Step 4, dilution, according to original liquid concentration, target liquid concentration, target liq product, the first peristaltic pump flow q1 and second
The flow value q2 of peristaltic pump, calculates the duration of runs T1 of the first peristaltic pump and duration of runs T2 of the second peristaltic pump；
Step 5, control device control alarm device warning reminding.
2. the dilution process of diluent facilities as claimed in claim 1, it is characterised in that be provided with drainage tube in the dilution bottle,
The upper end of the drainage tube is connected with pump line.
3. the dilution process of diluent facilities as claimed in claim 1, it is characterised in that the alarm device is loudspeaker or report
Warning lamp or vibrator.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410112034.XA CN103852369B (en) | 2014-03-24 | 2014-03-24 | Diluting device and method |
| PCT/CN2014/083209 WO2015143809A1 (en) | 2014-03-24 | 2014-07-29 | Dilution device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410112034.XA CN103852369B (en) | 2014-03-24 | 2014-03-24 | Diluting device and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103852369A CN103852369A (en) | 2014-06-11 |
| CN103852369B true CN103852369B (en) | 2017-05-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410112034.XA Active CN103852369B (en) | 2014-03-24 | 2014-03-24 | Diluting device and method |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN103852369B (en) |
| WO (1) | WO2015143809A1 (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103852369B (en) * | 2014-03-24 | 2017-05-17 | 深圳市清时捷科技有限公司 | Diluting device and method |
| CN104897820B (en) * | 2015-05-22 | 2017-01-25 | 中国科学院寒区旱区环境与工程研究所 | An online sample dilution and neutralization test device |
| CN111054458B (en) * | 2019-12-27 | 2025-01-17 | 珠海美华医疗科技有限公司 | High-precision micro-liquid filling equipment |
| CN111054227A (en) * | 2020-01-09 | 2020-04-24 | 张世奇 | A preparation device and preparation method for realizing solution dilution and preparation by utilizing peristaltic pump |
| CN111693397A (en) * | 2020-05-25 | 2020-09-22 | 广东省测试分析研究所（中国广州分析测试中心） | Novel automatic constant volume device and automatic constant volume method |
| CN113663547A (en) * | 2021-09-26 | 2021-11-19 | 陆越自动化科技(上海)有限公司 | Accurate online dilution system and dilution method thereof |
| CN115254221A (en) * | 2022-07-14 | 2022-11-01 | 启盘科技发展(上海)有限公司 | Solution adding device and using method thereof |
| CN117142414B (en) * | 2023-09-06 | 2025-07-25 | 广东先通分子影像科技有限公司 | Automatic nuclide distribution device |
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| JP3476417B2 (en) * | 2000-06-05 | 2003-12-10 | 株式会社島津製作所 | Analytical method by liquid chromatography |
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| KR100693799B1 (en) * | 2005-04-13 | 2007-03-12 | 최다희 | Circulating chemical fluid input method and system for maintaining and correcting chemical concentration in liquid |
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Also Published As
| Publication number | Publication date |
|---|---|
| WO2015143809A1 (en) | 2015-10-01 |
| CN103852369A (en) | 2014-06-11 |
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