CN103845343A - Application of pecan sterol extractive in preparation of anti-inflammatory materials - Google Patents

Abstract

The invention discloses the application of hickory nut sterol extract in preparing anti-inflammatory articles; the anti-inflammatory articles include anti-inflammatory drugs, daily cosmetics for anti-inflammation, or health products for anti-inflammation. The weight content of the total sterols in the hickory nut sterol extract is 10-50%, and the total sterols include β-sitosterol, rock soap sterol, campesterol and stigmasterol. The weight content of β-sitosterol in the total sterols is 70-95%, and the weight ratio between β-sitosterol, rock saponosterol, campesterol and stigmasterol is 70-95:1-10:2-12:1 ~15.

Description

Application of hickory nut sterol extract in preparation of anti-inflammatory articles

technical field

The invention relates to the field of deep processing and comprehensive utilization of agricultural and sideline products. More specifically, it relates to the use of a phytosterol extract extracted from hickory nuts.

Background technique

Hickory (Carya), also known as "small walnut" and walnut catalpa, is an endangered plant under national third-level protection. Juglandaceae, Juglans, originated in the Tertiary and Cretaceous, is one of the older groups of angiosperms. Hickory nuts grow in a natural environment with superior climate, fertile soil and lush vegetation. It is a pure wild fruit and is a natural green food without any pollution pollution. There are many types of hickory, about 30 species, mainly produced in America. In my country, there are 5 species of hickory in Lin'an, Dabie Mountain, Hunan, Guizhou and Yunnan. In addition, American hickory is introduced and cultivated from America. Among the hickory genus, American hickory and Lin'an hickory are the most planted. The annual output of American pecans is 78,000-18,000 tons, and the market value can reach 20 billion to 40 billion US dollars. Lin'an hickory is mainly produced in the Tianmu Mountains at the junction of Zhejiang and Anhui. Lin'an is one of the main producing areas of hickory in Zhejiang. As of 2011, the hickory planting area in Hangzhou City exceeded 800,000 mu, and the output value exceeded 2.3 billion yuan.

There are many types of natural active ingredients in pecans. In addition to basic nutrients, the main functional ingredients contained in pecans are: phenolic acids, flavonoids, steroids, terpenes, alkaloids, active polysaccharides, vitamins, etc., among which Mainly phenolic acids, tocopherols, sterols, etc. At present, the research on the active ingredients of pecans is mostly limited to the pecan varieties with the characteristics of domestic resources, especially the research on American pecans is more comprehensive and in-depth.

China is an ancient country of hickory production, utilization and research. However, the further development and utilization of pecan resources by domestic scientific researchers are still in the exploratory stage, such as using pecans to prepare dietary fiber, separating active proteins and polysaccharides, and developing high-nutrition feed.

Laura et al. (2010) studied that pecans are extremely rich in phenolic acids, mainly containing ellagic acid, gallic acid, chlorogenic acid, and p-hydroxybenzoic acid, of which ellagic acid is the main component. Margit et al. (2006) systematically studied the types and contents of tocopherols in 10 common nuts. The research showed that the tocopherols of American hickory nuts are mainly γ-tocopherols, and their content can reach more than 90% of the total tocopherols.

However, so far, many constituents and functions of hickory nuts are still poorly understood. In order to develop and utilize hickory nut resources more effectively, it is urgent to discover and develop various components with beneficial functions in hickory nut.

Phytosterol is a steroidal compound with a hydroxyl group in the 3 position, with cyclopentane perhydrophenanthrene as the main skeleton. There are many kinds of natural phytosterols, mainly including β-sitosterol, stigmasterol, brassicasterol and campesterol. Sterol. It is generally believed that vegetable oil and its processed products are the most abundant natural sources of phytosterols, followed by grain by-products and nuts. Corn oil, canola oil, rice bran oil, wheat germ oil, rye, wheat, barley and oats are all rich in sterols. Regarding the application of phytosterols, a large number of works have been published so far, covering the fields of medicine, cosmetics, food, feed, optical products, paints, pigments, resins, papermaking, textiles, insecticides and herbicides. Using phytosterols as raw materials, almost all steroid drugs can be synthesized, which is an industry with 4 billion US dollars worldwide. Therefore, the separation and purification of phytosterols have been highly valued.

Vegetable oil refining by-products, sugar by-products and papermaking waste liquids are rich in sterols. Among them, the by-products of vegetable oil refining are the main source of sterols. Extraction of sterols from raw materials is usually carried out in two steps. Crude sterols are first extracted from raw materials and then further refined. Common methods include solvent crystallization, molecular distillation, enzymatic, dry saponification, and complexation. But these processes are not ideal enough, or consume a large amount of flammable organic solvents, or the product purity is not high, or high temperature easily decomposes sterols.

At present, the total sterols of pecan extracts currently available only contain β-sitosterol and stigmasterol.

Contents of the invention

The technical problem to be solved by the present invention is to provide a phytosterol extract extracted from hickory nuts - the use of hickory nut sterol extract.

In order to solve the above technical problems, the present invention provides the application of hickory nut sterol extract in the preparation of anti-inflammatory articles. The anti-inflammatory articles include anti-inflammatory drugs, daily cosmetics for anti-inflammation, or health products for anti-inflammation.

As an improvement in the application of the pecan sterol extract of the present invention in the preparation of anti-inflammatory articles: the total sterol weight content in the pecan sterol extract is 10 to 50%, and the total sterols include β-sitosterol, rock Saponasterol, campesterol and stigmasterol (consisting basically of the above 4 sterols).

As a further improvement of the application of the pecan sterol extract of the present invention in the preparation of anti-inflammatory articles: the weight content of β-sitosterol in the total sterols is 70-95%, and β-sitosterol, rock soap sterol, campesterol, soybean The weight ratio between sterols is 70-95:1-10:2-12:1-15.

As a further improvement of the application of the pecan sterol extract of the present invention in the preparation of anti-inflammatory articles: the weight content of total sterols in the extract is 20-40%, and the weight content of β-sitosterol in the total sterols is 75-90% .

As a further improvement of the application of the hickory nut sterol extract of the present invention in the preparation of anti-inflammatory articles: the weight ratio between β-sitosterol, rock saponosterol, campesterol and stigmasterol is 75～90:2～10:2～ 10:2-11.

As a further improvement of the application of the pecan sterol extract of the present invention in the preparation of anti-inflammatory articles: the weight ratio between β-sitosterol, rock saponosterol, campesterol and stigmasterol is 76.5:9.6:2.9:11.

As a further improvement of the application of the pecan sterol extract of the present invention in the preparation of anti-inflammatory articles: the preparation method of the pecan sterol extract comprises the following steps:

1) Grinding hickory nuts (20-40 mesh) as raw material (water content about 5-10%, weight%), in the separation kettle, the raw material is subjected to supercritical fluid extraction under the following conditions: the extraction agent is CO _2. The extraction pressure is 15-35MPa, the extraction temperature is 40-70°C, the separation temperature is 30-50°C, the separation pressure is 4-8MPa, and the cycle dynamic extraction is 1-5h;

2) Take out the extract from the separation kettle and dry it (at 50-60°C for 40-60 minutes), so as to obtain the hickory nut sterol extract.

As a further improvement of the application of the pecan sterol extract of the present invention in the preparation of anti-inflammatory articles: the extraction pressure in step 1) is 20-30MP, the extraction temperature is 45-65°C, an entrainer is used, and the amount of the entrainer is 5% to 15% of the weight of hickory; the entrainer is a C1-C4 alcohol organic solvent (that is, any one of the C1-C4 alcohol organic solvent) or a C1-C4 ketone organic solvent ( That is, any one of C1-C4 ketone organic solvents).

As a further improvement of the application of the Cargo sterol extract of the present invention in the preparation of anti-inflammatory articles: the Cargo sterol extract is prepared into a composition, and the composition is composed of 0.01-99.9wt% Cargo sterol extract and the remaining amount of acceptable carrier composition.

As a further improvement of the application of the pecan sterol extract of the present invention in the preparation of anti-inflammatory articles: the composition is a pharmaceutical composition, a health product composition, a food composition or a cosmetic composition.

After many years of in-depth research, the inventor has extracted and separated various components of hickory nuts, and obtained an extract that can effectively reduce inflammation. The research on the extract shows that the extract mainly contains β-sitosterol, rock Compositions such as soap sterol, campesterol and stigmasterol have completed the present invention on this basis.

As used herein, hickory refers to the fruit of the hickory tree. The hickory nuts that can be used in the present invention can be hickory kernels, hickory skins, or wastes generated during hickory processing, such as hickory shells and waste fruits. Preferred starting materials are hickory shells and hickory kernels.

The raw materials used in the present invention may or may not be pretreated. A preferred starting material is pecan kernels in powder form. For example, hickory kernels, hickory skins, and hickory shells are collected, pulped, pressed or directly aired to a certain extent, then dried to a water content of about 5-10%, and crushed to about 20-40 mesh powder.

The hickory varieties that can be used in the present invention are not particularly limited, and may be different kinds of hickory, preferably Lin'an hickory and American hickory.

As used herein, "active substance of the present invention" refers to pecan sterol extract.

As used herein, "the active substance preparation of the present invention" or "the active composition of the present invention" refers to a composition containing the sterol extract of hickory nuts, including a pharmaceutical composition and a health product composition.

The pecan sterol extract of the present invention is preferably extracted by supercritical fluid extraction technology, although it can also be extracted by other methods.

A preferred method is to extract sterols from pecans and their processing waste (pecan shells) using supercritical fluid extraction. In a preferred mode, the extractant is CO ₂ , the extraction pressure is 15-35MPa, the extraction temperature is 40-70°C, the separation temperature is 30-50°C, the separation pressure is 4-8MPa, and alcohol can be added during the extraction process. Or ketone acts as an entrainer to play a synergistic effect.

In a more preferred way, put about 20-40 mesh hickory powder into the supercritical extraction kettle, the extraction agent is CO ₂ , CO ₂ is boosted by the compressor and the extraction kettle is heated at the same time, when the extraction pressure reaches 15~ 35MPa, when the extraction temperature reaches 40-70°C, the cycle starts; during the cycle, _CO2 and the extract are decompressed in the separator, and the extract is precipitated in the separator, and the _CO2 is recycled, and the separation tank 1 is set The separation pressure is set at 6-8MPa, the temperature is 35-45°C, the separation pressure of the separation tank 2 is set at 4-7MPa, and the temperature is 30-35°C; in the state of using or not using an entrainer during the extraction process, the cycle dynamic Extract for 1-5 hours, the entrainer used can be C1-C4 alcohol or ketone organic solvent (such as methanol, ethanol, acetone, etc.), and the dosage is generally 5-15% of the weight of the raw material. After the extraction is completed, take out the extract from the separation kettle, and after drying at low temperature, you can get the pecan sterol extract, and recover the entrainer from the separation kettle (in the state of using the entrainer), stop the machine and take out the extract from the extraction kettle remnants.

The pecan sterol extract of the present invention is a mixture mainly containing β-sitosterol, rockapasterol, campesterol and stigmasterol. Detect with Liebermann Burchard spectrophotometry, take β-sitosterol as standard substance, measure the content of total sterols in the extract to be 10～50% (preferably 20～40%); Analyze with UPLC technique, wherein β- The content of sitosterol (relative to the total sterols) is 70-95% (preferably 75-90%), and the ratio between β-sitosterol, rock saponosterol, campesterol and stigmasterol is 70-95:1～ 10:2～12:1～15. More preferably, it is 75-90:2-10:2-10:2-11.

The hickory nut sterol extract of the invention has beneficial effects such as anti-inflammation, so it can be used to prepare anti-inflammatory medicines, daily cosmetics and health care products. Therefore, the present invention also provides a composition (including pharmaceutical composition, health product composition, food composition and cosmetic composition, etc.) containing the hickory nut sterol extract of the present invention as an active ingredient. These compositions can be used to prevent, treat or assist in the treatment of inflammation.

After obtaining the pecan sterol extract, it can be mixed with pharmaceutically, food science, health care products or cosmetically acceptable carriers, excipients or diluents by conventional methods to form the pharmaceutical composition of the present invention, food Composition, health product composition or cosmetic composition. Such carriers include, but are not limited to: saline, buffer, dextrose, water, glycerol, ethanol, and combinations thereof.

Taking pharmaceutical compositions or health product compositions as an example, they can be in solid state (such as granules, tablets, freeze-dried powder, suppositories, capsules, sublingual tablets) or liquid state (such as oral liquid) or other suitable shapes. The content of the active ingredient of the present invention (Pecanus sterol extract)) is usually 0.01-99.9% of the weight of the composition, preferably 1-99%, more preferably 2-95%, and even more preferably 5-90%, optimally 10-80%.

The pharmaceutical compositions may be in single or multiple dose form. According to the administration dose, it usually contains 10-2000 mg/dose, preferably about 20-1000 mg/dose, more preferably 50-500 mg/dose.

The pharmaceutical composition of the present invention can be administered through conventional routes, preferably orally. The pharmaceutical formulation should match the mode of administration. The dosage of the medicament of the present invention is usually about 0.1-500 mg/kg body weight per day, preferably about 0.5-50 mg/kg body weight, calculated on the basis of the hickory nut sterol extract. In addition, the formulations of the present invention can also be used with other therapeutic agents, including, for example, various existing anti-inflammatory drugs.

The main advantages of the present invention are:

(a) Provide a pecan sterol extract with broad sources, clear composition, high quality, controllable quality, wide application and various physiological and pharmacological activities.

(b) The high-efficiency extraction of phytosterols in pecans and their processing wastes was realized by supercritical CO ₂ fluid extraction technology, and the process was easy to operate, and the obtained sterol extracts of pecans were of good and stable quality.

(c) Pecan sterol extract has anti-inflammatory properties.

Description of drawings

The specific implementation manners of the present invention will be described in further detail below in conjunction with the accompanying drawings.

Fig. 1 is the ultra high performance liquid phase chromatogram of Carya sterol extract;

Figure 2 Structural diagram of phytosterols in pecan sterol extract.

Detailed ways

Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the following examples, the conventional conditions or the conditions suggested by the manufacturer are usually followed.

general method

1) Determination of phytosterol content in pecan sterol extract——Liebermann Burchard colorimetric method

After the sample was extracted and dissolved with chloroform, the lower chloroform layer was removed. Accurately pipette 1ml of chloroform sample solution, add 2ml of acetic anhydride and 1 drop of concentrated sulfuric acid, shake well and measure the absorbance at 625nm wavelength with a 1cm cuvette. Each sample was measured twice in parallel, using β-sitosterol as a standard, converted to the corresponding total sterol content according to the standard curve, and taking the average value to obtain the total phytosterol content in the sample.

2) Analysis of active components of pecan sterol extract——Ultra Performance Liquid Chromatography (UPLC)

The sterol composition and content present in the extract were analyzed by using ultra-high performance liquid chromatography.

UPLC conditions: ultra-high performance liquid chromatography from Waters, ACQUITY BEH C18 column (2.1mm i.d.*150mm, 1.7μm, Waters), mobile phase is acetonitrile: water = 9:1, flow rate is 0.6mL/min, column temperature The temperature is 40°C, and the detection wavelength is 210nm. The detection scan range is from 210nm to 400nm.

The supercritical extraction apparatus selected in the following examples is a conventional one-extraction-two-partition _CO2 circulation type, including an extraction tank, a separation tank, a _CO2 storage tank, an entrainer pump, and the like.

Embodiment 1, a kind of preparation method of pecan sterol extract:

After shelling the Linan hickory nuts, beat the hickory nut kernels into pulp, dry them in the sun, dry them with microwave until the moisture content is 5% (weight %), crush them into 5kg of 20-mesh hickory nut powder as raw material, and make them Put it into a supercritical extraction kettle, raise the temperature to 50°C, start the plunger pump to pump CO ₂ into the extraction kettle through the preheater, and start circulation when the pressure reaches 25MPa; turn on the entrainer pump to make 10% of the raw material weight Ethanol enters the extraction tank after passing through the preheater; during the circulation process, _CO2 and the extract are decompressed in the separator, and the extract is precipitated in the separator, _CO2 is recycled, and the separation pressure is set in the separation tank 1 8MPa, temperature 40°C, separation tank 2 set separation pressure to 5MPa, temperature 30°C; after extraction for 4 hours, take out 60g of extract from separation tank 1 and 2, the yield is 1.2%, after low temperature drying (i.e. , after drying at 50-60°C for 50 minutes), 45g of pecan sterol extract was obtained, and the entrainer was recovered from the separation tank 1 and 2; the extraction tank was shut down to take out the raffinate.

The extract was analyzed for its components, and the results are shown in Figure 1-2.

Figure 1 shows that the components of the hickory nut sterol extract include: about 9 minutes is fusapesterol, about 11 minutes is campesterol, about 12 minutes is stigmasterol, and about 16 minutes is β-sitosterol.

Figure 2 confirms that the extract contains fucosterol, campesterol, stigmasterol, and β-sitosterol.

The UPLC method analyzes the component content of pecan sterol extract, and its total sterol content is 25.6%, and β-sitosterol is 19.6% (that is, the content of β-sitosterol in total sterol is 76.56%), β-sitosterol, rock The weight ratio among saponasterol, campesterol and stigmasterol is 76.5:9.6:2.9:11.

Embodiment 2, a kind of preparation method of pecan sterol extract:

The American hickory shells are preliminarily dried in the sun and then dried with hot air (70-90°C) until the moisture content is 5%, crushed into 5kg of 20-mesh hickory powder as raw material, and put it into a supercritical extraction kettle to heat up When the temperature reaches 60°C, start the plunger pump to pump CO ₂ into the extraction kettle through the preheater. When the pressure reaches 30MPa, start the circulation; turn on the entrainer pump to make 10% acetone pass through the preheater and enter the extraction kettle at the same time; During the circulation process, CO2 _and the extract are decompressed in the separator, and the extract is precipitated in the separator, and the _CO2 is recycled. The separation pressure of the separation tank 1 is set at 8MPa and the temperature is 45 ° C. The separation tank 2 Set the separation pressure to 5MPa and the temperature to 30°C; after extraction for 2.5 hours, take out 35g of the extract from separation kettles 1 and 2, with a yield of 0.7%, and obtain 31g of pecan extract after drying at low temperature, and extract from Recover the entrainer in the separation kettle 1 and 2; stop the machine and take out the raffinate from the extraction kettle.

The extract is subjected to component analysis, and the result is the same as in Example 1. The content of the extract was analyzed by UPLC. The total sterol content was 31.7%, the β-sitosterol content was 25.4% (that is, the content of β-sitosterol in the total sterols was 80.12%), β-sitosterol, rock saponosterol , campesterol, stigmasterol ratio is 80.2:3.5:6.4:9.9.

Embodiment 3, a kind of preparation method of pecan sterol extract:

Grind the hickory kernels from Yunnan into pulp, press to remove water and preliminarily dry them in the sun, then dry them with microwaves until the water content is 5%, crush them into 6kg of 20-mesh hickory kernel powder, and put them into a supercritical extraction kettle , raise the temperature to 45°C, start the plunger to pump CO ₂ into the extraction kettle through the preheater, and start the cycle when the pressure reaches 20MPa; during the cycle, CO ₂ and the extract are decompressed in the separator, and the The extract is precipitated in the separator, and the _CO2 is recycled. The separation pressure of the separation kettle 1 is set to 6MPa and the temperature is 35°C, and the separation pressure of the separation kettle 2 is set to 5MPa and the temperature is 30°C; 60g of the extract was taken out in 1 and 2, and the yield was 1.0%. After drying at low temperature, 49g of the pecan sterol extract was obtained; the machine was shut down and the raffinate was taken out from the extraction kettle.

The extract is subjected to component analysis, and the result is the same as in Example 1. The content of the extract was analyzed by UPLC. The total sterol content was 37.1%, and the β-sitosterol content was 32.8% (that is, the content of β-sitosterol in the total sterols was 88.4%). The ratio between campesterol and stigmasterol is 88.5:2:7.5:2.

Embodiment 4, a kind of preparation method of pecan sterol extract:

Grind Hunan hickory nuts and shells, beat them into pulp, press to remove water, dry them in the sun, dry them with microwave until the moisture content is 5%, grind them into 20 mesh powder 6kg, and put them into a supercritical extraction kettle , heat up to 50°C, start the plunger pump to pump CO ₂ into the extraction kettle through the preheater, and start circulation when the pressure reaches 15MPa; turn on the entrainer pump, so that 10% ethanol enters the extraction tank at the same time after passing through the preheater Extraction tank; during the circulation process, CO2 _and the extract are decompressed in the separator, and the extract is precipitated in the separator, and _CO2 is recycled. The separation pressure of the separation tank 1 is set at 7MPa and the temperature is 40°C. The separation pressure of separation kettle 2 is set at 5MPa and the temperature is 30°C; after extraction for 2.5h, 90g of extract is taken out from separation kettle 1 and 2, and the yield is 1.8%. After low-temperature drying, 78g of pecan sterol extraction material, recover the entrainer from the separation tank 1, 2, shut down and take out the raffinate from the extraction tank.

The extract is subjected to component analysis, and the result is the same as in Example 1. The content of the extract was analyzed by UPLC method, the total sterol content was 27.3%, the β-sitosterol content was 22.4% (that is, the content of β-sitosterol in the total sterol was 82.05%), β-sitosterol, rock saponosterol , campesterol, stigmasterol ratio is 81.9:6.5:3.8:7.8.

Embodiment 5, a kind of preparation method of pecan sterol extract:

Chop the Guizhou hickory nut skin and preliminarily dry it in the sun, dry it with microwave until the moisture content is 5%, crush it into 5kg of 20-mesh powder, put it into a supercritical extraction kettle, raise the temperature to 50°C, and start the plunger pump Pump CO ₂ into the extraction tank through the preheater. When the pressure reaches 25MPa, the circulation starts; during the circulation process, CO ₂ and the extract are decompressed in the separator, and the extract is separated in the separator. _CO2 is recycled, the separation pressure of separation kettle 1 is set to 8MPa, the temperature is 45°C, the separation pressure of separation kettle 2 is set to 5MPa, and the temperature is 30°C; after 4 hours of extraction, 45g of extract is taken out from separation kettle 1 and 2 , The yield was 0.9%. After drying at low temperature, 37g of the pecan sterol extract was obtained. Stop the machine and take out the raffinate from the extraction kettle.

The extract is subjected to component analysis, and the result is the same as in Example 1. The content of the extract was analyzed by UPLC. The total sterol content was 22.9%, and the β-sitosterol content was 19.8% (that is, the content of β-sitosterol in the total sterols was 86.46%). , campesterol, stigmasterol ratio is 86.6:2:8.7:3.6.

Activity test 1, the Cargo sterol extract obtained in embodiment 1 is carried out to the following performance detection

Anti-inflammatory effects of pecan sterol extract:

1) Effect on ear swelling of mice induced by croton oil

The mice were randomly divided into 5 groups according to body weight, 10 in each group, and the settings were as follows: ①Blank control group: equal volume of normal saline, 0.8mL/20g; ②Positive control group: indomethacin 10mg/kg; ③High-dose group: 200mg/kg kg of pecan sterol extract; ④ medium dose group: 100 mg/kg of pecan sterol extract; ⑤ low dose group: 50 mg/kg of pecan sterol extract. Animals were administered once a day for 7 consecutive days (indomethacin was only administered once, and normal saline was used for the next 6 days). 1 hour after the last administration, the right ear of the mouse was coated with 0.02mL of 2% croton oil to induce inflammation for 4 hours Afterwards, they were put to death, and the left and right ears were cut along the auricles, and the left and right ear pieces were punched out with a 9mm trephine drill, and weighed. The difference in weight between the left and right ear pieces was used as the swelling rate.

Remarks: The above-mentioned hickory nut sterol extract (and "indomethacin") of the present invention need not be dissolved in physiological saline, and can be administered directly.

The experimental results showed that after intragastric administration of 100mg/kg and 200mg/kg doses of pecan sterol extract to mice for 7 days, it had significant (p<0.05) and extremely significant (p<0.01) effects on the ear swelling caused by croton oil. ) in a dose-dependent manner.

Table 1 Effect of hickory nut sterol extract on ear swelling caused by croton oil in mice

^* p<0.05, ^** p<0.01, compared to the control group.

2) Effect of p-xylene-induced ear swelling in mice

The mice were randomly divided into 5 groups according to body weight, 10 in each group, and the setting was the same as 1 above. The animals were administered once a day for 7 consecutive days (indomethacin was only administered once). 30 minutes after the last administration, the right ear of the mouse was coated with 0.05 mL of xylene to cause inflammation. After 15 minutes, it was killed, and the left and right ears were cut along the auricle. For ears, the left and right ear pieces were punched out with a 9mm trephine, weighed, and the difference in weight between the left and right ear pieces was used as the swelling rate. And compared with the control group to judge the curative effect.

Table 2 shows that after intragastric administration of 400 mg/kg of hickory nut sterol extract to mice for 7 consecutive days, it has a significant (p<0.05) inhibitory effect on auricle swelling caused by xylene, and the effect is not obvious at medium and low doses .

Table 2 Effect of hickory nut sterol extract on ear swelling caused by xylene in mice

^* p<0.05, ^** p<0.01, compared to the control group.

The invention adopts the supercritical _CO2 fluid extraction technology to extract the phytosterol extract from the hickory nut and its processing waste. Not only the process is easy to operate, but also the quality of the obtained pecan sterol extract is good and stable. Moreover, the extract has good effects of anti-inflammation, lowering blood fat and blood cholesterol, etc., and has wide application prospects in cosmetics, food, health care products and medicines.

Activity test 2, the method that the pecan sterol extract that above-mentioned embodiment gains is equal to above-mentioned activity test 1 detects, and obtained result is as follows:

Table 3, the effect of hickory nut sterol extract on the auricle swelling caused by mouse croton oil

^* p<0.05, ^** p<0.01, compared to the control group.

Table 4, the effect of hickory nut sterol extract on mouse auricle swelling caused by xylene

^* p<0.05, ^** p<0.01, compared to the control group.

Finally, it should be noted that the above examples are only some specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and many variations are possible. All deformations that can be directly derived or associated by those skilled in the art from the content disclosed in the present invention should be considered as the protection scope of the present invention.

Claims (10)

1. the application of hickory nut sterol extract in the preparation anti-inflammatory article. 2. the application of pecan sterol extract according to claim 1 in the preparation of anti-inflammatory articles is characterized in that: the total sterol weight content is 10～50% in the described pecan sterol extract, and described total sterol Including beta-sitosterol, rock soap sterol, campesterol and stigmasterol. 3. the application of pecan sterol extract according to claim 2 in the preparation anti-inflammatory article is characterized in that: the weight content of β-sitosterol is 70-95% in the total sterols, and β-sitosterol, rock soap The weight ratio among sterol, campesterol and stigmasterol is 70-95:1-10:2-12:1-15. 4. the application of pecan sterol extract according to claim 3 in the preparation anti-inflammatory article is characterized in that: total sterol weight content is 20-40% in this extract, and the weight of β-sitosterol in total sterol The content is 75-90%. 5. the application of pecan sterol extract according to claim 4 in the preparation anti-inflammatory article is characterized in that: the weight ratio between β-sitosterol, rock saponosterol, campesterol, stigmasterol is 75～90: 2～10: 2～10: 2～11. 6. the application of pecan sterol extract according to claim 5 in the preparation anti-inflammatory article is characterized in that: the weight ratio between β-sitosterol, rock soap sterol, campesterol, stigmasterol is 76.5:9.6: 2.9:11. 7. according to the application of the hickory nut sterol extract described in any one of claims 1 to 6 in the preparation of anti-inflammatory articles, it is characterized in that: the preparation method of the hickory nut sterol extract comprises the following steps: 1) The hickory nuts are crushed as raw materials. In the separation kettle, the raw materials are subjected to supercritical fluid extraction under the following conditions: the extraction agent is CO ₂ , the extraction pressure is 15-35 MPa, the extraction temperature is 40-70°C, and the separation The temperature is 30-50°C, the separation pressure is 4-8 MPa, and the dynamic extraction is 1-5 hours in circulation; 2) Take out the extract from the separation kettle and dry it to obtain the pecan sterol extract. 8. the application of pecan sterol extract according to claim 7 in the preparation anti-inflammatory article is characterized in that: The extraction pressure in step 1) is 20-30MP, the extraction temperature is 45-65°C, and an entrainer is used. The amount of the entrainer is 5-15% of the weight of the hickory; the entrainer is C1-C4 alcohol Organic solvents or C1-C4 ketone organic solvents. 9. The application of the hickory nut sterol extract according to any one of claims 1 to 6 in the preparation of anti-inflammatory articles, characterized in that: the hickory nut sterol extract is prepared into a composition, and the composition consists of 0.01-99.9wt % of pecan sterol extract and acceptable carrier as the balance. 10. The application of the pecan sterol extract according to claim 9 in the preparation of anti-inflammatory articles, characterized in that: the composition is a pharmaceutical composition, a health product composition, a food composition or a cosmetic composition.

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