CN103819572A - Extraction technology for production of polysaccharide from mulberry leaf - Google Patents
Extraction technology for production of polysaccharide from mulberry leaf Download PDFInfo
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- CN103819572A CN103819572A CN201410063375.2A CN201410063375A CN103819572A CN 103819572 A CN103819572 A CN 103819572A CN 201410063375 A CN201410063375 A CN 201410063375A CN 103819572 A CN103819572 A CN 103819572A
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- polysaccharide
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- extraction
- polysaccharides
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 22
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 22
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 22
- 238000000605 extraction Methods 0.000 title claims abstract description 19
- 240000000249 Morus alba Species 0.000 title claims abstract description 15
- 235000008708 Morus alba Nutrition 0.000 title claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 title abstract description 7
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 239000012535 impurity Substances 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 14
- 238000001556 precipitation Methods 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 4
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical class [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000002351 wastewater Substances 0.000 claims description 3
- 229920001661 Chitosan Polymers 0.000 claims description 2
- 108010010803 Gelatin Proteins 0.000 claims description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims description 2
- 229920000159 gelatin Polymers 0.000 claims description 2
- 239000008273 gelatin Substances 0.000 claims description 2
- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- 229920002401 polyacrylamide Polymers 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 16
- 238000005516 engineering process Methods 0.000 abstract description 5
- MFYSUUPKMDJYPF-UHFFFAOYSA-N 2-[(4-methyl-2-nitrophenyl)diazenyl]-3-oxo-n-phenylbutanamide Chemical compound C=1C=CC=CC=1NC(=O)C(C(=O)C)N=NC1=CC=C(C)C=C1[N+]([O-])=O MFYSUUPKMDJYPF-UHFFFAOYSA-N 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000002932 luster Substances 0.000 description 2
- 238000000874 microwave-assisted extraction Methods 0.000 description 2
- 238000002137 ultrasound extraction Methods 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 241001518926 Cladrastis Species 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 241000218231 Moraceae Species 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000003699 antiulcer agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 235000007063 yellowwood Nutrition 0.000 description 1
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention relates to an extraction technology for production of polysaccharide from mulberry leaf, especially to a method for extracting and separating high-purity polysaccharide from mulberry leaf. The invention adopts a method for removing impurities with a precipitating agent. The method is an extraction technology for production of polysaccharide from mulberry leaf, and the method has advantages of simple technology, high efficiency and high purity. By the adoption of the technology provided by the invention, a light-yellow high-purity mulberry-leaf polysaccharide product can be prepared at a time, and the purity can reach up to more than 95%. Production time occupied by the chromatogram process adopted for preparing a high-purity product by a conventional technology can be saved in quantity.
Description
One, technical field
The present invention relates to the extraction process that a kind of polysaccharides of Folium Mori is produced, a kind of particularly method of extracting separating high-purity polysaccharides of Folium Mori from mulberry leaf.
Two, technical background
Mulberry leaf, it is the leaf of moraceae plants mulberry, mulberry tree has another name called a mulberry, Jing Sang, mulberry fruit tree, yellow wood leaf etc., whole nation most area has production more, has the effects such as anticoagulation, hypotensive, blood fat, decreasing cholesterol, antithrombotic formation and atherosclerosis, antiviral, antitumor, anti-ageing, antifatigue, spasmolysis, antiulcer agent, anti-inflammatory; Especially polysaccharides of Folium Mori, has effect significant hypoglycemic and that inhibition blood fat raises
Production technology and the technique of from mulberry leaf, extracting at present polysaccharides of Folium Mori are more, as: water extraction, ultrasonic extraction, microwave extraction, Enzymatic Extraction, the methods such as ultra-high voltage extraction are wherein the highest with ultrasonic extraction and microwave extraction yield, but the equal purity of the product of no matter taking which kind of extracting method gained is very low, product conventional content is conventionally in 20% left and right, and appearance luster is yellowish green, impure also many, can only serve as the mixed type pharmaceutical preparation of low side, not be suitable for the method for the opening application of high-end preparation; Therefore the content and the purity that improve product are particularly important.
Three, summary of the invention
The object of this invention is to provide the extraction process that a kind of polysaccharides of Folium Mori is produced, its technology is to adopt precipitation agent to go the technique of deimpurity method, is the extraction process that simple, efficient, the highly purified a kind of polysaccharides of Folium Mori of a kind of technique is produced.
The extraction process that a kind of polysaccharides of Folium Mori of the present invention is produced, is completed by following steps:
(1), by mulberry leaf raw material pulverizing to 1---5 order;
(2), add purifying waste water of 10 times of raw material weights, under the temperature condition of 75 ℃, pump circulation is extracted 3 times continuously, each 3 hours extraction times; United extraction liquid;
(3), add deployed precipitation agent to precipitate in extracting solution, the add-on of precipitation agent is extracting solution weight 5%, leaves standstill after 12 hours, and centrifugation is filtered and removed impurity, obtains filtered liquid;
(4), add the activated carbon powder of filtered liquid weight 2% in filtered liquid, at 60 ℃ of-75 ℃ of temperature, stir 30 minutes, after absorption soluble impurity, recentrifuge separating and filtering;
(5), by the filtered liquid after separating and filtering again, at 75 ℃ of-90 ℃ of temperature, when its weight 1/10 of vacuum concentration, add concentrated solution 5-10 doubly to measure high purity ethanol, carry out alcohol precipitation 12 hours;
(6), precipitation solution filtering separation, holds back solid part and with high purity ethanol washing, obtains polysaccharide goods;
(7), the polysaccharide vacuum dehydrating at lower temperature of gained, dried product is crushed to Powdered molecule;
(8), detect active constituent content, packing.
Described precipitation agent is by polymerize aluminum chloride 20%, polyacrylamide 5%, and gelatin 10%, calcium carbonate 5%, chitosan 60% forms, and after above raw material is merged and mixed, adds 4 times of pure water at 60 ℃ of temperature, to be stirred to completely and dissolves, and is statically placed under normal temperature for subsequent use.
Described high purity ethanol is the ethanol of 90--95%.
Technique provided by the invention can be accomplished disposable high-purity mulberry leaf polyose of producing the micro-Huang of color and luster, and purity, up to more than 95%, can be saved the shared production time of chromatography processes link that common process high-purity product adopts in a large number.
Four, embodiment:
Below in conjunction with embodiment, the present invention is done furtherly and carefully described.
Embodiment 1:
(1), take 100 grams of the mulberry leaf raw materials of pulverizing;
(2), add 10 times of raw material weight to purify waste water 1000 milliliters, under the temperature condition of 75 ℃, pump circulation is extracted 3 times continuously, each 3 hours extraction times, united extraction liquid;
(3), add 5 milliliters of deployed precipitation solvent to precipitate in extracting solution, leave standstill centrifugation after 12 hours and filter the solid impurity of removing absorption, obtain filtered liquid;
(4), filtered liquid adds the activated carbon powder of 2 grams recentrifuge separating and filtering after 30 minutes absorption soluble impurities of continuously stirring and pigment at 65 ℃ of temperature;
(5), filtered liquid is concentrated into 10 milliliters under 80 ℃ of vacuum conditions, adds the high purity ethanol of 50 milliliters of content 95% to carry out alcohol precipitation, the time is 12 hours;
(6), precipitation solution filtering separation, hold back the part of solid and with content 95% washing with alcohol to the colourless polysaccharide goods that obtain of washings;
(7), the polysaccharide goods vacuum dehydrating at lower temperature of gained, weigh 7.8 grams of gained after dry, colourity is slightly yellowish, reaches more than 95% through UV detection level;
(8), packing.
Claims (3)
1. the extraction process that polysaccharides of Folium Mori is produced, is characterized in that: its following steps complete:
(1), by mulberry leaf raw material pulverizing to 1---5 order;
(2), add purifying waste water of 10 times of raw material weights, under the temperature condition of 75 ℃, pump circulation is extracted 3 times continuously, each 3 hours extraction times; United extraction liquid;
(3), add deployed precipitation agent to precipitate in extracting solution, the add-on of precipitation agent is extracting solution weight 5%, leaves standstill after 12 hours, and centrifugation is filtered and removed impurity, obtains filtered liquid;
(4), add the activated carbon powder of filtered liquid weight 2% in filtered liquid, at 60 ℃ of-75 ℃ of temperature, stir 30 minutes, after absorption soluble impurity, recentrifuge separating and filtering;
(5), by the filtered liquid after separating and filtering again, at 75 ℃ of-90 ℃ of temperature, when its weight 1/10 of vacuum concentration, add concentrated solution 5-10 doubly to measure high purity ethanol, carry out alcohol precipitation 12 hours;
(6), precipitation solution filtering separation, holds back solid part and with high purity ethanol washing, obtains polysaccharide goods;
(7), the polysaccharide vacuum dehydrating at lower temperature of gained, dried product is crushed to Powdered molecule;
(8), detect active constituent content, packing.
2. the extraction process that a kind of polysaccharides of Folium Mori as claimed in claim 1 is produced, it is characterized in that: precipitation agent is by polymerize aluminum chloride 20%, polyacrylamide 5%, gelatin 10%, calcium carbonate 5%, chitosan 60% forms, and above raw material is merged after mixing, add 4 times of pure water at 60 ℃ of temperature, to be stirred to completely and dissolve, be statically placed under normal temperature for subsequent use.
3. the extraction process that a kind of polysaccharides of Folium Mori as claimed in claim 1 is produced, is characterized in that: the ethanol that high purity ethanol is 90--95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410063375.2A CN103819572A (en) | 2014-02-17 | 2014-02-17 | Extraction technology for production of polysaccharide from mulberry leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410063375.2A CN103819572A (en) | 2014-02-17 | 2014-02-17 | Extraction technology for production of polysaccharide from mulberry leaf |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103819572A true CN103819572A (en) | 2014-05-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410063375.2A Pending CN103819572A (en) | 2014-02-17 | 2014-02-17 | Extraction technology for production of polysaccharide from mulberry leaf |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104403016A (en) * | 2014-11-17 | 2015-03-11 | 安徽工程大学 | Folium mori polysaccharide as well as separation and purification preparation method and application thereof |
| CN104829737A (en) * | 2015-04-30 | 2015-08-12 | 华南理工大学 | Crude raspberry leaf polysaccharide, and preparation method and application thereof |
| CN104877038A (en) * | 2015-05-28 | 2015-09-02 | 北京石油化工学院 | Method of extracting polysaccharide from mulberry leaves |
| CN105085700A (en) * | 2015-08-25 | 2015-11-25 | 北京石油化工学院 | Method for extracting, separating and purifying polysaccharide from mulberry leaves |
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| CN101121920A (en) * | 2007-08-01 | 2008-02-13 | 陕西省科学院酶工程研究所 | Preparation method for biological flocculant |
| CN101214279A (en) * | 2008-01-09 | 2008-07-09 | 浙江工商大学 | A method for extracting flavonoids and polysaccharides from mulberry leaves |
| CN101412703A (en) * | 2008-11-17 | 2009-04-22 | 江苏科技大学 | Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid |
| CN101709278A (en) * | 2009-11-23 | 2010-05-19 | 浙江省环境保护科学设计研究院 | Large-scaled culture method of high-concentration nitrosobacteria and application thereof |
| CN101712726A (en) * | 2009-10-30 | 2010-05-26 | 浙江工业大学 | Method for preparing high-purity mulberry leaf polysaccharide |
| CN102774947A (en) * | 2012-07-25 | 2012-11-14 | 华南理工大学 | Biological compound flocculant and application thereof |
| CN103524632A (en) * | 2013-03-21 | 2014-01-22 | 王喜军 | Rapid preparation process of astragalus polysaccharide |
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2014
- 2014-02-17 CN CN201410063375.2A patent/CN103819572A/en active Pending
Patent Citations (8)
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| JP2006160862A (en) * | 2004-12-07 | 2006-06-22 | Oki Bussan:Kk | Method for extracting fucoidan |
| CN101121920A (en) * | 2007-08-01 | 2008-02-13 | 陕西省科学院酶工程研究所 | Preparation method for biological flocculant |
| CN101214279A (en) * | 2008-01-09 | 2008-07-09 | 浙江工商大学 | A method for extracting flavonoids and polysaccharides from mulberry leaves |
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| CN101712726A (en) * | 2009-10-30 | 2010-05-26 | 浙江工业大学 | Method for preparing high-purity mulberry leaf polysaccharide |
| CN101709278A (en) * | 2009-11-23 | 2010-05-19 | 浙江省环境保护科学设计研究院 | Large-scaled culture method of high-concentration nitrosobacteria and application thereof |
| CN102774947A (en) * | 2012-07-25 | 2012-11-14 | 华南理工大学 | Biological compound flocculant and application thereof |
| CN103524632A (en) * | 2013-03-21 | 2014-01-22 | 王喜军 | Rapid preparation process of astragalus polysaccharide |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104403016A (en) * | 2014-11-17 | 2015-03-11 | 安徽工程大学 | Folium mori polysaccharide as well as separation and purification preparation method and application thereof |
| CN104403016B (en) * | 2014-11-17 | 2016-09-07 | 安徽工程大学 | A kind of mulberry leaf polysaccharide and preparation method for separating and purifying thereof and application |
| CN104829737A (en) * | 2015-04-30 | 2015-08-12 | 华南理工大学 | Crude raspberry leaf polysaccharide, and preparation method and application thereof |
| CN104829737B (en) * | 2015-04-30 | 2017-09-29 | 华南理工大学 | A kind of palmleaf raspberry leaf Thick many candies and preparation method and application |
| CN104877038A (en) * | 2015-05-28 | 2015-09-02 | 北京石油化工学院 | Method of extracting polysaccharide from mulberry leaves |
| CN105085700A (en) * | 2015-08-25 | 2015-11-25 | 北京石油化工学院 | Method for extracting, separating and purifying polysaccharide from mulberry leaves |
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Application publication date: 20140528 |