CN103815287B - A kind of imitative whole wheat fermented type alimentary paste and preparation method thereof - Google Patents
A kind of imitative whole wheat fermented type alimentary paste and preparation method thereof Download PDFInfo
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- CN103815287B CN103815287B CN201410034313.9A CN201410034313A CN103815287B CN 103815287 B CN103815287 B CN 103815287B CN 201410034313 A CN201410034313 A CN 201410034313A CN 103815287 B CN103815287 B CN 103815287B
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/109—Types of pasta, e.g. macaroni or noodles
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
技术领域 technical field
本发明属于食品技术领域,具体涉及一种仿全麦发酵型营养面条及其制作方法。 The invention belongs to the technical field of food, and in particular relates to a whole-wheat imitation fermented nutritional noodle and a preparation method thereof.
背景技术 Background technique
面条是一种非常古老的食物,起源于中国,有着源远流长的历史。在中国东汉年间已经存在记载,至今超过一千九百年。目前市场上有许多种面条,包括各种各样的挂面、方便面等,尽管制作配方和具体工艺略有差别,但其本质均与传统的面条制作方法类似,营养功能成分没有得到改善。例如现有技术中公开了“一种营养保健面条的制作方法”(申请号/专利号:200610200466),主要由面粉、蛋白多肽、红花提取物、苦瓜提取物、野酸梅提取物份和小麦多肽制作而成。孙茂合的“一种营养面条”(申请号/专利号:200610043741.3),只是在传统面条的制作配方中添加了牛奶、胡椒粉和牛骨汤,其他均无变化。宋其详研究了“一种皮子面的制作方法”(申请号/专利号:200910191530.8),在面条中加入了50g山楂,经30分钟发酵而成,因发酵时间短没有形成发酵面团应有的特点。宋胜广等的“一种保健营养面条”(申请号/专利号:CN03114488.8),仅是在面团中加入一定比例的芦荟汁和魔芋粉。 Noodles are a very ancient food that originated in China and has a long history. It has been recorded in the Eastern Han Dynasty of China, and it has been more than 1,900 years. There are many kinds of noodles on the market, including various dried noodles, instant noodles, etc. Although there are slight differences in production formula and specific process, its essence is all similar to traditional noodle production methods, and the nutritional and functional components have not been improved. For example, the prior art discloses "A Method for Making Nutritious and Healthy Noodles" (Application No./Patent No.: 200610200466), which mainly consists of flour, protein polypeptide, safflower extract, bitter melon extract, wild sour plum extract and Made from wheat peptides. Sun Maohe's "A Kind of Nutritious Noodles" (Application No./Patent No.: 200610043741.3) only added milk, pepper and beef bone broth to the traditional noodle recipe, with no other changes. Song Qixiang researched "a method of making leather noodles" (application number/patent number: 200910191530.8). He added 50g of hawthorn to the noodles and fermented them for 30 minutes. Due to the short fermentation time, the characteristics of fermented dough were not formed. "A kind of health-care and nutritious noodles" (application number/patent number: CN03114488.8) of Song Shengguang etc. only adds a certain proportion of aloe vera juice and konjac powder in the dough.
我国2010版《中国食物成分表》中,麦麸主要的可溶性营养成分如下:B1含量达0.3mg/100g,B2达0.3mg/100g,磷682mg/100g、硒7.12μg/100g、钾682mg/100g、钙206mg/100g、锌5.98mg/100g、铜2.03mg/100g、锰10.85mg/100g,以及多种氨基酸等等。 In China's 2010 edition of "Chinese Food Composition Table", the main soluble nutrients of wheat bran are as follows: B 1 content up to 0.3mg/100g, B 2 up to 0.3mg/100g, phosphorus 682mg/100g, selenium 7.12μg/100g, potassium 682mg /100g, calcium 206mg/100g, zinc 5.98mg/100g, copper 2.03mg/100g, manganese 10.85mg/100g, and a variety of amino acids and so on.
目前市场上已经有全麦面包、全麦饼干等产品,但尚未见到全麦面条,究其原因可能是直接添加麦麸会直接影响面条的口感,利用麦麸提取液和面经发酵后制作仿全麦营养保健面条的方法也未见报道。 At present, there are whole-wheat bread, whole-wheat biscuits and other products on the market, but no whole-wheat noodles have been seen. The reason may be that the direct addition of wheat bran will directly affect the taste of noodles. The method of imitating whole wheat nutritional health noodles has not been reported yet.
王华等研究表明,小麦中赖氨酸、苏氨酸和异亮氨酸含量较低,是小麦中的限制性氨基酸。其中小麦中赖氨酸含量为3.65mg/g,苏氨酸含量为4.09mg/g,异亮氨酸含量为4.47mg/g。而核桃粕中赖氨酸含量约为16.5mg/g,苏氨酸含量为16.5mg/g,异亮氨酸含量为20.3mg/g。通过添加核桃粕粉,可以改善小麦中的限制性氨基酸含量,从而提高小麦的营养价值。 Studies by Wang Hua and others have shown that the content of lysine, threonine and isoleucine in wheat is low, which are the limiting amino acids in wheat. Among them, the content of lysine in wheat is 3.65mg/g, the content of threonine is 4.09mg/g, and the content of isoleucine is 4.47mg/g. The lysine content in walnut meal is about 16.5 mg/g, the threonine content is 16.5 mg/g, and the isoleucine content is 20.3 mg/g. By adding walnut meal powder, the limiting amino acid content in wheat can be improved, thereby improving the nutritional value of wheat.
据研究资料和试验研究表明,发酵后的面团许多营养成分比未发酵的要高,并且更容易消化吸收,并且发酵后的酵母还是一种很强的抗氧化物,可以保护肝脏,还有一定的解毒作用。面团经过发酵,蛋白质含量增加;面团发酵过程中影响钙、镁、铁等元素吸收的植酸可被分解,从而提高人体对这些矿质营养素的吸收和利用;据我们研究发现经过面团发酵,其中的多酚含量提高了13.6%。多酚是一种天然抗氧化剂,有研究表明多酚能有效清除人体内的自由基,对癌症和衰老起到预防作用,能够降低心脑血管、糖尿病等疾病风险性,具有抗菌、消炎及抗病毒的功效。 According to research data and experimental studies, many nutrients of fermented dough are higher than those of unfermented dough, and it is easier to digest and absorb, and fermented yeast is also a strong antioxidant, which can protect the liver and has a certain detoxification. After the dough is fermented, the protein content increases; during the dough fermentation process, the phytic acid that affects the absorption of calcium, magnesium, iron and other elements can be decomposed, thereby improving the absorption and utilization of these mineral nutrients by the human body; according to our research, it is found that after dough fermentation, the The polyphenol content increased by 13.6%. Polyphenol is a natural antioxidant. Studies have shown that polyphenol can effectively remove free radicals in the human body, prevent cancer and aging, and reduce the risk of cardiovascular and cerebrovascular diseases, diabetes and other diseases. It has antibacterial, anti-inflammatory and anti-inflammatory properties. The efficacy of the virus.
发明内容 Contents of the invention
本发明的目的是在现有面条制作的基础上,不增加生产成本,主要通过调整生产工艺来提高面条的营养成分,提供了一种仿全麦发酵型营养面条及其制作方法。 The purpose of the present invention is to improve the nutritional content of the noodles mainly by adjusting the production process without increasing the production cost on the basis of the existing noodles, and to provide a whole-wheat imitation fermented nutritional noodles and a production method thereof.
本发明采用如下的技术方案实现: The present invention adopts following technical scheme to realize:
一种仿全麦发酵型营养面条,由以下组分的原料加工而成:面粉100kg、食盐0.1~1Kg、酵母0.3~1kg、麦麸10Kg、核桃粕粉2.8~3.2kg。 A whole-wheat imitation fermented nutritional noodle is processed from the following raw materials: 100kg of flour, 0.1-1kg of salt, 0.3-1kg of yeast, 10Kg of wheat bran, and 2.8-3.2kg of walnut meal powder.
各原料的重量组分最佳配比为:面粉100kg、食盐0.5Kg、酵母0.5kg、麦麸10Kg、核桃粕粉3kg。 The optimal ratio of weight components of each raw material is: 100kg of flour, 0.5Kg of salt, 0.5kg of yeast, 10Kg of wheat bran and 3kg of walnut meal powder.
制备方法为:麦麸用1:25、温度为25-35℃的水室温条件下浸泡1小时,过滤,上清液用于和面;原料各组分混合均匀后于35~37℃条件下发酵1.5~3h,加入碱面调节面团的酸度,然后压片、切面。 The preparation method is as follows: soak wheat bran in 1:25 water at 25-35°C for 1 hour at room temperature, filter, and use the supernatant for kneading dough; Ferment for 1.5-3 hours, add alkaline noodles to adjust the acidity of the dough, then press into sheets and cut into noodles.
本发明实施过程中,按比例称取原料,放入容器内混合、搅拌、压片、切面等,本发明既可以加工成挂面、方便面,也可以加工成各种新鲜面条。 During the implementation of the present invention, the raw materials are weighed in proportion, put into a container for mixing, stirring, tableting, cutting noodles, etc. The present invention can be processed into dried noodles, instant noodles, or various fresh noodles.
按照本发明的配方生产出的面条,营养丰富,煮熟时间短,煮熟后不断条、口感绵软滑润、容易消化吸收,特别适用于老年人和儿童食用。 The noodles produced according to the formula of the invention are rich in nutrition, short in cooking time, unbroken after cooking, soft and smooth in taste, easy to digest and absorb, and are especially suitable for eating by the elderly and children.
本发明中:麦麸含有丰富的膳食纤维,是人体必需的营养元素,可提高食物中的纤维成分,可改善大便秘结情况,同时可促使脂肪及氮的排泄,对临床常见纤维缺乏性疾病的防治作用意义重大;由于摄入了高纤维成分,从而可以降低粪便中的类固醇的排出,而人体内胆固醇的主要分界代谢过程是通过粪便的排泄,所以可以使血清胆固醇下降,动脉粥样硬化的形成减慢;可有助于预防结肠及直肠癌;可降低血液中雌激素的含量,可预防乳腺癌;麦麸中含有的B族维生素,在体内发挥着许多功能,而且还是食物正常代谢中不可缺少的营养成分。氧化是机体衰老的最大威胁,而麦麸提取液对DPPH·的清除能力较强,具有较强的抗氧化活性;具有较强的还原能力。麦麸通过发酵将不溶性的膳食纤维转化成可溶的膳食纤维,使面条更加易于消化。 In the present invention: wheat bran is rich in dietary fiber, which is an essential nutrient element for the human body, can increase the fiber content in the food, can improve the constipation of the stool, and can promote the excretion of fat and nitrogen at the same time, and can treat common clinical fiber deficiency diseases. The prevention and treatment effect of Cholesterol is of great significance; due to the intake of high-fiber ingredients, it can reduce the excretion of steroids in the feces, and the main boundary metabolic process of cholesterol in the human body is excretion through feces, so it can reduce serum cholesterol and prevent atherosclerosis. It can help prevent colon and rectal cancer; it can reduce the content of estrogen in the blood and prevent breast cancer; the B vitamins contained in wheat bran perform many functions in the body, and they are also the normal metabolism of food indispensable nutrients. Oxidation is the biggest threat to the aging of the body, and the extract of wheat bran has a strong ability to scavenge DPPH·, has a strong antioxidant activity, and has a strong reducing ability. Wheat bran converts insoluble dietary fiber into soluble dietary fiber through fermentation, making noodles easier to digest.
作为压榨或萃取制油后的副产物,核桃粕中蛋白含量占核桃干重的30%~50%。核桃蛋白中18种氨基酸齐全,8种必需氨基酸含量适中,是一种优质的蛋白资源,但其利用率却很低,常被作为饲料低价出售;本发明中使用核桃粕所制粉不仅脂肪含量明显减少,而且提高了面条中的蛋白质含量,充分利用了农产品加工后的副产物,即提高了产品的营养成分,又提高了其他作物的综合利用价值,由于在制作过程中,将面粉进行了发酵,核桃粕中的蛋白质酶解为小分子的蛋白质或者氨基酸,利于人体的吸收。 As a by-product after pressing or extracting oil, the protein content in walnut meal accounts for 30% to 50% of the walnut dry weight. In walnut protein, 18 kinds of amino acids are complete, and the content of 8 kinds of essential amino acids is moderate. It is a high-quality protein resource, but its utilization rate is very low, and it is often sold as feed at a low price; The protein content in the noodles is significantly reduced, and the protein content in the noodles is improved, and the by-products after the processing of agricultural products are fully utilized, which not only improves the nutritional content of the products, but also improves the comprehensive utilization value of other crops. After fermentation, the protein in the walnut meal is enzymatically hydrolyzed into small molecular proteins or amino acids, which is conducive to the absorption of the human body.
本发明在现有面条制作的基础上,不增加生产成本,通过调整生产工艺来提高面条的营养成分。该面条不仅加工工艺简单,生产成本低,而且营养丰富。据初步研究表明,面条口感好,容易消化吸收,特别适用于老年人和儿童食用。 Based on the existing noodle production, the invention does not increase the production cost, and improves the nutritional content of the noodle by adjusting the production process. The noodles are not only simple in processing technology, low in production cost, but also rich in nutrition. According to preliminary research, the noodles have a good taste and are easy to digest and absorb, and are especially suitable for the elderly and children.
本发明通过添加麦麸提取液的方式,即达到营养回补,又不影响感官质量。本营养面条制作工艺一方面通过发酵提高营养成分和改善消化吸收率及其保健功能,另一方面用麦麸提取液和面可以强化面粉在磨制中损失的矿物质和维生素。为提高面条中的蛋白质有效利用率,加入核桃榨油后的核桃粕粉等。通过对农产品加工后的副产物进行充分利用,即提高了产品的营养成分,又提高了其他作物的综合利用价值。 The present invention achieves nutrition replenishment without affecting the sensory quality by adding the wheat bran extract. On the one hand, the production process of the nutritious noodles improves the nutrient content, digestion and absorption rate and health function through fermentation, and on the other hand, the wheat bran extract liquid and the noodles can strengthen the minerals and vitamins lost in the milling of the flour. In order to improve the effective utilization rate of protein in noodles, walnut meal powder after walnut oil extraction and the like are added. By making full use of the by-products after agricultural product processing, not only the nutritional content of the product is improved, but also the comprehensive utilization value of other crops is improved.
附图说明 Description of drawings
图1为不同浓度的乙醇对麦麸多酚提取效果的影响;图2为对二苯代苦味酰基自由基(DPPH·)清除能力的测定结果;图3为对高价铁还原力的测定结果。 Fig. 1 is the effect of different concentrations of ethanol on the extraction effect of wheat bran polyphenols; Fig. 2 is the measurement result of the scavenging ability of diphenylpicryl radical (DPPH·); Fig. 3 is the measurement result of the reducing power of high-valent iron.
具体实施方式 Detailed ways
为详细说明本发明,下面结合具体实施例对本发明做进一步详细描述。关于和面用水量,可以根据最终产品的不同进行调整。 In order to illustrate the present invention in detail, the present invention will be further described in detail below in conjunction with specific examples. Regarding the amount of water used for kneading dough, it can be adjusted according to the difference of the final product.
实施例1:一种仿全麦发酵型营养面条,称取面粉100kg、食盐0.1Kg、酵母0.3kg、麦麸10Kg、核桃粕粉2.8kg。麦麸用1:25、温度为25℃的水室温条件下浸泡1小时,过滤,上清液用于和面;原料各组分混合均匀后于35℃条件下发酵1.5h后,压片、切面制成挂面、方便面或新鲜面条。所用核桃粕粉是将核桃榨油后的核桃粕粉碎过40目筛所得。 Example 1: A whole-wheat imitation fermented nutritional noodle, weighing 100kg of flour, 0.1Kg of salt, 0.3kg of yeast, 10Kg of wheat bran, and 2.8kg of walnut meal powder. Wheat bran is soaked in 1:25 water at 25°C for 1 hour at room temperature, filtered, and the supernatant is used for kneading dough; the components of the raw materials are mixed evenly and fermented at 35°C for 1.5 hours, then pressed into tablets, Cut noodles to make dried noodles, instant noodles or fresh noodles. The used walnut meal powder is obtained by crushing the walnut meal after walnut oil extraction through a 40-mesh sieve.
实施例2:一种仿全麦发酵型营养面条,称取面粉100kg、食盐0.5Kg、酵母0.5kg、麦麸10Kg、核桃粕粉3kg。麦麸用1:25、温度为30℃的水室温条件下浸泡1小时,过滤,上清液用于和面;原料各组分混合均匀后于36℃条件下发酵2h后加入碱面,搅拌均匀,压片、切面制成挂面、方便面或新鲜面条。所用核桃粕粉是将核桃榨油后的核桃粕粉碎过40目筛所得。 Example 2: A whole-wheat imitation fermented nutritional noodle, weighing 100 kg of flour, 0.5 kg of salt, 0.5 kg of yeast, 10 kg of wheat bran, and 3 kg of walnut meal powder. Soak the wheat bran in 1:25 water at 30°C for 1 hour at room temperature, filter, and use the supernatant for kneading dough; after mixing all components of the raw materials, ferment at 36°C for 2 hours, add alkaline flour, and stir Uniform, pressed into slices, cut into noodles, instant noodles or fresh noodles. The used walnut meal powder is obtained by crushing the walnut meal after walnut oil extraction through a 40-mesh sieve.
实施例3:一种仿全麦发酵型营养面条,称取面粉100kg、食盐0.6Kg、酵母0.6kg、麦麸10Kg、核桃粕粉3.1kg。麦麸用1:25、温度为35℃的水室温条件下浸泡1小时,过滤,上清液用于和面;原料各组分混合均匀后于37℃条件下发酵2.5h后加入碱面,搅拌均匀,压片、切面制成挂面、方便面或新鲜面条。所用核桃粕粉是将核桃榨油后的核桃粕粉碎过40目筛所得。 Example 3: A whole-wheat imitation fermented nutritional noodle, weighing 100 kg of flour, 0.6 kg of salt, 0.6 kg of yeast, 10 kg of wheat bran, and 3.1 kg of walnut meal powder. Soak the wheat bran in 1:25 water at 35°C for 1 hour at room temperature, filter, and use the supernatant for kneading dough; after mixing the raw materials evenly, ferment at 37°C for 2.5 hours, then add alkaline noodles, Stir evenly, press into pieces, and cut into noodles to make dried noodles, instant noodles or fresh noodles. The used walnut meal powder is obtained by crushing the walnut meal after walnut oil extraction through a 40-mesh sieve.
实施例4:一种仿全麦发酵型营养面条,称取面粉100kg、食盐1Kg、酵母1kg、麦麸10Kg、核桃粕粉3.2kg。麦麸用1:25、温度为32℃的水室温条件下浸泡1小时,过滤,上清液用于和面;原料各组分混合均匀后于37℃条件下发酵3h后加入碱面,搅拌均匀,压片、切面制成挂面、方便面或新鲜面条。所用核桃粕粉是将核桃榨油后的核桃粕粉碎过40目筛所得。 Example 4: A whole-wheat imitation fermented nutritional noodle, weighing 100kg of flour, 1Kg of salt, 1kg of yeast, 10Kg of wheat bran, and 3.2kg of walnut meal powder. Soak the wheat bran in 1:25 water at 32°C for 1 hour at room temperature, filter, and use the supernatant for kneading dough; after mixing the ingredients evenly, ferment at 37°C for 3 hours, add alkaline flour, and stir Uniform, pressed into slices, cut into noodles, instant noodles or fresh noodles. The used walnut meal powder is obtained by crushing the walnut meal after walnut oil extraction through a 40-mesh sieve.
实验例1:为强化面条中的矿物质、维生素B2和多酚类物质的含量,用麦麸的水提取物和面。通常多酚类物质均采用乙醇等有机溶剂辅助提取,为了比较二者的差异,做了如下试验进行比较。 Experimental Example 1 : In order to strengthen the content of minerals, vitamin B2 and polyphenols in noodles, water extract of wheat bran was used to knead noodles. Usually, polyphenols are extracted with the assistance of organic solvents such as ethanol. In order to compare the difference between the two, the following experiments were done for comparison.
具体方法是:将麦麸用1:25、温度为25-35℃的水室温条件下浸泡1小时,过滤,上清液备用;同时,在与水浸提条件相同的情况下,用不同浓度的乙醇对同样量的麦麸浸泡、过滤,提取上清液,研究不同浓度的乙醇对麦麸多酚的提取效果,结果见图1。 The specific method is: soak wheat bran in 1:25 water at 25-35°C for 1 hour at room temperature, filter, and use the supernatant for later use; at the same time, under the same conditions as water extraction, use different concentrations The same amount of ethanol was soaked and filtered, and the supernatant was extracted to study the extraction effect of different concentrations of ethanol on wheat bran polyphenols. The results are shown in Figure 1.
由图1可知,不同浓度的乙醇溶液对多酚类物质的提取率不同,其中体积分数为10%的乙醇溶液提取率最高。而采用水做提取液,多酚提取率为5.21mg/g,是10%乙醇溶液提取量的86.3%。因此,为了不增加生产成本,采用水来浸泡麦麸在理论上是可行的。另一方面,口感方面克服了直接使用麦麸带来的粗糙感,风味方面克服了直接使用麦麸带来的麦麸味,色泽方面也远远优于直接添加麦麸所生产的面条。总之,从面条的色香味方面来考虑,添加麦麸提取液都要优于直接添加麦麸所生产的面条。 It can be seen from Figure 1 that the extraction rates of polyphenols are different for ethanol solutions with different concentrations, and the ethanol solution with a volume fraction of 10% has the highest extraction rate. While using water as the extracting solution, the polyphenol extraction rate is 5.21mg/g, which is 86.3% of the extraction amount of the 10% ethanol solution. Therefore, in order not to increase production costs, it is theoretically feasible to use water to soak wheat bran. On the other hand, in terms of taste, it overcomes the rough feeling brought by direct use of wheat bran, in terms of flavor, it overcomes the taste of wheat bran brought by direct use of wheat bran, and in terms of color, it is far superior to the noodles produced by directly adding wheat bran. In a word, considering the color, flavor and flavor of noodles, the addition of wheat bran extract is better than the noodles produced by directly adding wheat bran.
实验例2:对麦麸提取液的抗氧化活性的初步研究: Experimental example 2: Preliminary research on the antioxidant activity of wheat bran extract:
1.对二苯代苦味酰基自由基(DPPH·)清除能力的测定:具塞试管中加入2ml浓度为2×10-4mol/L的DPPH·无水乙醇溶液,再加入2ml抗氧化剂(不同浓度梯度的麦麸提取液、VC、BHT)的乙醇溶液,混匀,室温下静置30min后,517nm处测得吸光值A2,同时测定2ml浓度为2×10-4mol/L的DPPH·无水乙醇溶液与2ml无水乙醇混合液的吸光值A0,以及2ml抗氧化剂与2ml无水乙醇混合液的吸光值A1。计算DPPH·自由基的清除率: 1. Determination of the scavenging ability of diphenylpicric acyl radicals (DPPH ): Add 2ml of DPPH absolute ethanol solution with a concentration of 2×10 -4 mol/L in a stoppered test tube, then add 2ml of antioxidants (different concentration gradients) Wheat bran extract, V C , BHT) ethanol solution, mix well, after standing at room temperature for 30min, the absorbance value A 2 is measured at 517nm , and 2ml of DPPH· The absorbance value A 0 of the mixture of absolute ethanol solution and 2ml of absolute ethanol, and the absorbance value A 1 of the mixture of 2ml of antioxidant and 2ml of absolute ethanol. Calculate the scavenging rate of DPPH free radicals:
DPPH·自由基的清除率(%)=[A0-(A2-A1)]/A0×100。 DPPH·free radical scavenging rate (%)=[A 0 -(A 2 -A 1 )]/A 0 ×100.
式中:A0—2.0mLDPPH·溶液+2.0mL无水乙醇的吸光度值;A1—2.0mL样品溶液+2.0mL无水乙醇的吸光度值;A2—2.0mL DPPH·溶液+2.0mL样品溶液的吸光度值。 In the formula: A 0 —the absorbance value of 2.0mL DPPH solution+2.0mL absolute ethanol; A 1 —the absorbance value of 2.0mL sample solution+2.0mL absolute ethanol; A 2—2.0mL DPPH·solution+2.0mL sample solution absorbance value.
实验结果如图2所示,对DPPH·的清除能力排序为:50%乙醇洗脱液(麦麸提取液纯化物1)>30%乙醇洗脱液(麦麸提取液纯化物2)>VC>BHT;并且,随着浓度的增加,上述四种物质对DPPH·的清除能力逐渐增强,抗氧化性增强。可见,麸皮提取物对DPPH·的清除能力要优于VC和BHT,且50%洗脱液的清除能力要强于30%洗脱液。 The experimental results are shown in Figure 2. The scavenging ability of DPPH is ranked as follows: 50% ethanol eluate (purified wheat bran extract 1)>30% ethanol eluate (purified wheat bran extract 2)>V C >BHT; and, with the increase of the concentration, the scavenging ability of the above four substances to DPPH· was gradually enhanced, and the antioxidant activity was enhanced. It can be seen that the scavenging ability of bran extract on DPPH· is better than that of V C and BHT, and the scavenging ability of 50% eluent is stronger than that of 30% eluent.
2.高价铁还原力的测定:吸取2.5mL浓度为20mmol/L的FeCl3·6H2O溶液,与2.5mL浓度为10mmol/L的TPTZ溶液和25mL浓度为300mmol/L(pH3.6)的醋酸盐缓冲液混合。吸取该混合液1.8mL于37℃水浴中,依次加入180μL蒸馏水和60μL抗氧化剂(各浓度梯度的麦麸提取液、VC、BHT),反应30min后,在593nm下读取吸光度值A。FRAP值越大,表明由抗氧化物质还原的Fe2+越多,即该物质的抗氧化活性越强。Vc和BHT做为阳性对照,以FeSO4溶液做标准曲线。结果表示为mg Fe(II)/g干重。通过线性回归分析可得FeSO4的浓度(X)与其在593nm处吸光值(Y)的回归方程为y=0.4742 x+0.0181,相关系数R2=0.9971。 2. Determination of the reducing power of high-valent iron: draw 2.5mL of FeCl 3 6H 2 O solution with a concentration of 20mmol/L, and 2.5mL of TPTZ solution with a concentration of 10mmol/L and 25mL of TPTZ solution with a concentration of 300mmol/L (pH3.6). Acetate buffer was mixed. Pipette 1.8 mL of the mixture into a water bath at 37°C, add 180 μL of distilled water and 60 μL of antioxidants (wheat bran extract, V C , BHT with different concentration gradients) in sequence, react for 30 minutes, and read the absorbance value A at 593 nm. The larger the FRAP value, the more Fe 2+ is reduced by the antioxidant substance, that is, the stronger the antioxidant activity of the substance. Vc and BHT were used as positive controls, and the standard curve was made with FeSO 4 solution. Results are expressed as mg Fe(II)/g dry weight. Through linear regression analysis, the regression equation between FeSO 4 concentration (X) and its absorbance value at 593nm (Y) is y=0.4742 x+0.0181, and the correlation coefficient R 2 =0.9971.
实验结果如图3所示,以上抗氧化因子按照FRAP值排序为:50%乙醇洗脱液(麦麸提取液纯化物1)>30%乙醇洗脱液(麦麸提取液纯化物2)>VC>BHT。研究结果表明在本试验研究条件下,相同浓度的抗氧化因子中,麦麸提取液的抗氧化能力最强。 The experimental results are shown in Figure 3. The above antioxidant factors are ranked according to the FRAP value: 50% ethanol eluate (purified wheat bran extract 1)>30% ethanol eluate (wheat bran extract purified 2)> V C >BHT. The research results show that under the conditions of this experiment, among the same concentration of antioxidant factors, the antioxidant capacity of wheat bran extract is the strongest.
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