CN103804511A - Method for preparing carboxymethyl yeast glucan product and product obtained by using same - Google Patents
Method for preparing carboxymethyl yeast glucan product and product obtained by using same Download PDFInfo
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Abstract
The invention relates to a method for preparing a carboxymethyl yeast glucan product and a product obtained by using the same. The preparation method comprises the following steps: adding alkali to yeast glucan according to the ratio that per 1kg of yeast glucan contains 12.5-37.5mol of hydroxyl; simultaneously adding ethanol according to the mass ratio of the yeast glucan to the ethanol being 1:(0.5-2.5), and reacting at 0-30 DEG C; adding a chloroacetic acid to the obtained solution according to the mass ratio of the yeast glucan to the chloroacetic acid being 1:(0.3-1.0), simultaneously adding ethanol according to the mass ratio of the yeast glucan to the ethanol being 1:(0.5-1.5), reacting at 30-80 DEG C and cooling; and adding ethanol in the obtained solution to wash, simultaneously neutralizing by using a hydrochloric acid, washing and centrifugally separating to obtain sediment carboxymethyl yeast glucan. By adopting the carboxymethyl yeast glucan product obtained by the method, the substitution degree is 0.3-1.0, the molecular weight is 100-1,000KD, and the carboxymethyl yeast glucan product is completely dissolved into water, and has the effects of moisturizing, preventing aging and the like when being applied to the field of cosmetics.
Description
Technical field
The present invention relates to preparation method and its product of preparing of a kind of carboxymethyl yeast glucan product.
Background technology
Yeast glucan is a kind of functional polysaccharide being present in yeast cells wall, has strengthening immunity, anti-infective, antitumor texts, can be applied to the fields such as food, makeup, Animal nutrition.The dextran that comes from yeast has special three-dimensional spiral structure, cannot dissolve, thereby limited its range of application in every field in water.
At present, there is bibliographical information to adopt a large amount of Isopropanol Solvents to prepare Sensor Chip CM 5, in technique, adopt a large amount of Virahols as solvent (what slurry method), the product substitution value 0.55 that the method for adding alkali twice prepares.Substitution value refers to the hydroxy number being replaced by reaction reagent on average each dehydration glucose unit, is called substitution value.
Virahol security is poor, adopts standby a large amount of solvents, the adding alkali twice method complex process of consuming of what slurry legal system.Visible, all there is certain shortcoming in aforesaid method.
Summary of the invention
The object of the invention is to prepare a kind of carboxymethyl derivant product of yeast glucan, the substitution value of this product is 0.3-1.0, and molecular weight is 100-1000KD, in water, dissolves completely, is applied to cosmetic field and has stronger moisturizing, the anti-ageing effect of waiting for a long time.
The present invention takes ethanol as solvent, change the reaction density of yeast glucan, and alkali, ethanol, Mono Chloro Acetic Acid etc. are adjusted with the mass ratio of yeast glucan, the novel method of preparing carboxymethyl yeast glucan product that research obtains, the method can be saved reagent, and obtains excellent replacement effect, and the molecular weight product obtaining is stable, substitution value is high, has stronger moisturizing, anti-ageing effect.
The preparation method who the present invention relates to a kind of carboxymethyl yeast glucan product, comprises the following steps:
A alkalization: add alkali according to every 1kg yeast glucan containing the ratio of hydroxide radical 12.5～37.5mol in yeast glucan, be that 1:0.5～2.5 add ethanol according to yeast glucan and ethanol mass ratio, in 0～30 ℃ of reaction simultaneously;
B etherificate: in the solution obtaining in steps A, be that 1:0.3～1.0 add Mono Chloro Acetic Acid by yeast glucan and chloroacetic mass ratio, be that 1:0.5～1.5 add ethanol by yeast glucan and ethanol mass ratio simultaneously, in 30～80 ℃ of reactions, cooling;
C washing: in the solution obtaining at step B, add washing with alcohol, with hydrochloric acid neutralization, washing, separates and be precipitated carboxymethyl yeast glucan simultaneously;
Preferably, also comprise following filtration step or drying step:
D filters: the Sensor Chip CM 5 that step C is obtained is dissolved in water, adopts diatomite filtration to obtain carboxymethyl yeast glucan liquid product, and while preferably making Sensor Chip CM 5 concentration be 1%-4%, its 600nm OD value is less than 0.4;
E is dry: the carboxymethyl yeast glucan that step C is obtained dry, pulverize and obtains carboxymethyl yeast glucan powder-like product, be preferably dried to moisture to be less than≤8%.
Preferably, the yeast glucan of described steps A is 1:0.7～1.0, most preferably 1:0.7 with the mass ratio of the alkali adding.
Preferably, the yeast glucan of described steps A is 1:1.0～2.0, most preferably 1:1.0 with the mass ratio of the ethanol adding.
Preferably, the yeast glucan of described step B and chloroacetic mass ratio are 1:0.5～0.8, most preferably 1:0.5.
Preferably, the yeast glucan of described step B is 1:0.5～1.0, most preferably 1:0.5 with the mass ratio of the ethanol adding.
Preferably, the yeast glucan of described step C is 1:3～1 0 with adding the mass ratio of ethanol; Preferably 1:8～1 0, most preferably 1:8.
Preferably, each step alcohol concn used is 70～95%.
Preferably, the alkali of described steps A is NaOH or KOH, preferably pure NaOH or KOH.
Preferably, the temperature of reaction of described steps A is 0～20 ℃, preferably 0-10 ℃, most preferably 0 ℃.
Preferably, the temperature of reaction of described step B is 40～70 ℃, preferably 50-60 ℃, most preferably 50 ℃.
Preferably, described steps A quaternization and step B etherification reaction all carry out in kneader.
The invention still further relates to carboxymethyl yeast glucan product prepared by above-mentioned method, substitution value 0.3～1.0, preferably 0.5～0.75, molecular weight 100-1000KD, is preferably 200-500KD, in water, dissolves completely.
Preferably, substitution value 0.3～1.0, preferably 0.5～0.75, molecular weight 100-1000KD, is preferably 200-500KD, in water, dissolves completely.
The Sensor Chip CM 5 product that present method obtains is because substitution value is suitable, and molecular weight is moderate, and it is applied to cosmetic field and has stronger moisturizing, the anti-ageing effect of waiting for a long time.
Accompanying drawing explanation
Fig. 1 is the Sensor Chip CM 5 infrared spectrogram of embodiment 5.
Fig. 2 is sample sets and the blank moisture content change situation of organizing after the sample of embodiment 5 uses.
Fig. 3 is sample sets and the blank changing conditions of organizing moisture loss after the sample of embodiment 5 uses.
Embodiment
Describe in detail with following example as follows, but, one skilled in the art will appreciate that protection scope of the present invention should not be confined to this.
Raw material of the present invention is the yeast glucan product that yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) or other kind yeast prepare, and this raw material can be from market purchase or by yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) or other kind yeast are obtained through extracting to separate.(hydroxyl value 4, molecular weight ranges 500,000～1,000,000)
Ethanol: use mainly as solvent, the product of capable of choosing multiple concentration, the present invention generally adopts 70～95% ethanol.
Mono Chloro Acetic Acid: the present invention uses analytically pure Mono Chloro Acetic Acid.
1) alkalization: be equipped with a certain amount of yeast glucan in kneader, adding the ratio of 12.5～37.5mol alkali to add alkali, add concentration by the mass ratio 1:0.5-1:2.5 of yeast glucan and ethanol according to every 1kg yeast glucan is the ethanol of 70%-95%, control temperature at 0-30 ℃, reaction continues 10-90min left and right; The alkali of described steps A is preferably pure NaOH or KOH; Temperature is preferably 0-20 ℃, most preferably 0-10 ℃; Preferred 1:0.7～1.0 of mass ratio of yeast glucan and the alkali that adds; Preferred 1:1.0～2.0 of mass ratio of yeast glucan and the ethanol that adds;
2) etherificate: be that 1:0.3-1:1.0 adds Mono Chloro Acetic Acid by dextran and chloroacetic mass ratio in kneader, the ethanol that the concentration that to add with dextran mass ratio be 1:0.5-1:1.5 is simultaneously 70%-95%, control temperature at 30-80 ℃, after reaction 10-90min, cooling finishes reaction; Temperature of reaction is preferably 40-70 ℃, most preferably 50～60 ℃; Yeast glucan and preferred 1:0.5～0.8 of chloroacetic mass ratio; Preferred 1:0.5～1.0 of mass ratio of yeast glucan and the ethanol that adds;
3) washing: from kneader, take out the said products, the ethanol that the concentration that to add by dextran and ethanol mass ratio in product be 1:3-1:10 is 70%-95%, simultaneously with hydrochloric acid neutralization, washing, centrifugation precipitates, and obtains Sensor Chip CM 5 product; Described washing, centrifugal repeating 1～5 time; Yeast glucan and preferred 1:8～10 of mass ratio that add ethanol;
Preferably, also can carry out to the Sensor Chip CM 5 product obtaining that filtration step obtains liquid product or drying step obtains powder-product:
4) filter: the Sensor Chip CM 5 product that step 3) is obtained is dissolved in water, adjust concentration 1%-4%, adopt diatomite filtration, make the optical density value (OD value) of its 600nm be less than 0.4, can make liquid product substantially keep transparent, sensory evaluation is higher, can be packaged as liquid product.
5) dry: the Sensor Chip CM 5 product that step 3) is obtained is dried, and obtains carboxymethyl yeast glucan powder-like product; Preferably be dried to moisture≤8%.
The raw materials used source of following examples is as shown in table 1 below:
The source of table 1 raw material or reagent and model
| Source commodity title | Producer or source | Model |
| Yeast glucan | Angel Yeast Co.,Ltd | G70 |
| Mono Chloro Acetic Acid | Tianjin good fortune chemical reagent factory in morning | Analytical pure |
| Sodium hydroxide | Tianjin Kermel Chemical Reagent Co., Ltd. | Analytical pure |
Model and the producer of following examples instrument or equipment are as shown in table 2 below:
The model of table 2 instrument or equipment and producer
Take yeast glucan 100kg and be placed in kneader, add therein analytical pure NaOH150kg, 70% ethanol 250kg, controlling temperature is 25 ℃ of reaction 90min.Then add Mono Chloro Acetic Acid 100kg, and add 70% ethanol 150kg, holding temperature to lower the temperature and finish reaction after 30 ℃ of reaction 90min.
From kneader, take out Sensor Chip CM 5, add the ethanol of 300kg70%, centrifuging and taking precipitation after washing.Adopt aforesaid way repeated washing, centrifugal 3 times, with salt acid for adjusting pH be neutrality, after collecting precipitation, oven drying to moisture is 5.8%, packs to obtain carboxymethyl yeast glucan powder-like product after pulverizing.
Yeast glucan 100kg is placed in kneader, adds therein analytical pure NaOH50kg, 85% ethanol 50kg, and controlling temperature is 30 ℃ of reaction 50min.Then add Mono Chloro Acetic Acid 30kg, and add 85% ethanol 50kg, holding temperature to lower the temperature and finish reaction after 80 ℃ of reaction 60min.
From kneader, take out Sensor Chip CM 5, add the ethanol of 1000kg85%, centrifuging and taking precipitation after washing.Adopt aforesaid way repeated washing, centrifugal 3 times, be neutral with salt acid for adjusting pH, after collecting precipitation, be dissolved in water, regulating weight percentage concentration is 2%, adopting diatomite filtration to OD(600nm) value is 0.3, utilize ultraviolet-visible pectrophotometer to measure the OD value of 600nm, directly pack to obtain carboxymethyl yeast glucan liquid product.
Yeast glucan 100kg is placed in kneader, add therein analytical pure NaOH100kg, 70% ethanol 200kg, controlling temperature is 20 ℃ of reaction 60min, then add Mono Chloro Acetic Acid 80kg and add 70% ethanol 100kg, holding temperature is that after 70 ℃ of reaction 60min, cooling finishes reaction.
From kneader, take out Sensor Chip CM 5, add the ethanol of 800kg70%, centrifuging and taking precipitation after washing.Adopt aforesaid way repeated washing, centrifugal 5 times, with salt acid for adjusting pH be neutrality, after collecting precipitation, oven drying to moisture is 5.1%, packs to obtain carboxymethyl yeast glucan powder-like product after pulverizing.
Yeast glucan 100kg is placed in kneader, add therein analytical pure NaOH70kg, 95% ethanol 100kg, controlling temperature is 10 ℃ of reaction 70min, then add Mono Chloro Acetic Acid 50kg and add 95% ethanol 50kg, holding temperature is that after 40 ℃ of reaction 90min, cooling finishes reaction.
From kneader, take out Sensor Chip CM 5, add the ethanol of 80kg95%, centrifuging and taking precipitation after washing.Adopting aforesaid way repeated washing, centrifugal 2 times, is neutral with salt acid for adjusting pH, is dissolved in water after collecting precipitation, controls content 1%, and after diatomite filtration, recording OD value is 0.18, directly packs, and obtains carboxymethyl yeast glucan liquid product.
Embodiment 5
Yeast glucan 100kg is placed in kneader, adds therein analytical pure NaOH70kg, 70% ethanol 100kg, controlling temperature is 0 ℃ of reaction 90min, then add Mono Chloro Acetic Acid 50kg, and add 70% ethanol 50kg, holding temperature to lower the temperature and finish reaction after 50 ℃ of reaction 90min.
From kneader, take out Sensor Chip CM 5, add the ethanol of 800kg70%, centrifuging and taking precipitation after washing.Adopt aforesaid way repeated washing, centrifugal 5 times, with salt acid for adjusting pH be neutrality, after collecting precipitation, oven drying to moisture is 6.3%, packs to obtain carboxymethyl yeast glucan powder-like product after pulverizing.
Embodiment 6
Yeast glucan 100kg is placed in kneader, add therein analytical pure NaOH80kg, 95% ethanol 150kg, controlling temperature is to add Mono Chloro Acetic Acid 70kg and add 95% ethanol 70kg after 10 ℃ of reaction 90min, holding temperature is that after 60 ℃ of reaction 90min, cooling finishes reaction.
From kneader, take out Sensor Chip CM 5, add the ethanol of 800kg95%, centrifuging and taking precipitation after washing.Adopting aforesaid way repeated washing, centrifugal 5 times, is neutral with salt acid for adjusting pH, is dissolved in water after collecting precipitation, controls content 2%, and after diatomite filtration, recording OD value is 0.24, directly packs, and obtains carboxymethyl yeast glucan liquid product.
Reference examples 1
Yeast glucan 100kg is placed in reactor, adds therein analytical pure NaOH150kg, 70% ethanol 1000kg, and controlling temperature is 25 ℃ of reaction 90min.Then add Mono Chloro Acetic Acid 100kg, and add 70% ethanol 150kg, holding temperature to lower the temperature and finish reaction after 30 ℃ of reaction 90min.
Add the ethanol of 300kg70%, centrifuging and taking precipitation after washing.Adopt aforesaid way repeated washing, centrifugal 3 times, with salt acid for adjusting pH be neutrality, after collecting precipitation, oven drying to moisture is 5.1%, packs to obtain carboxymethyl yeast glucan powder-like product after pulverizing.
Reference examples 2(what slurry method)
Yeast glucan 100kg adds analytical pure NaOH300kg, 95% ethanol 1000kg, and controlling temperature is to add Mono Chloro Acetic Acid 350kg after 20 ℃ of reaction 60min, holding temperature is that after 80 ℃ of reaction 120min, cooling finishes reaction.
Add the ethanol of 1000kg70%, centrifuging and taking precipitation after washing.Adopt aforesaid way repeated washing, centrifugal 5 times, with salt acid for adjusting pH be neutrality, after collecting precipitation, oven drying to moisture is 5%, packs to obtain carboxymethyl yeast glucan powder-like product after pulverizing.
Detect test:
One, infrared spectra
Method for making sample: KBr pressed disc method;
Instrument: FT-IR SPECTROMETER, Fourier transformation infrared spectrometer
Model: NICOLET NEXUS470, DTGS detector
Scanning times: 32 times, resolving power: 4cm
-1
The infrared spectra of the product that embodiment 1-6 obtains all can be found out, at 1602cm
-1there is obvious absorption peaks at place, is expressed as carboxymethylation product.Wherein, the product infrared spectra of embodiment 5 is shown in accompanying drawing 1.
Two, substitution value is measured
With reference to GB GB1904-2005(foodstuff additive---Xylo-Mucine) in the measuring method of substitution value.
The measurement result of the substitution value of reference examples and each embodiment is shown in as shown in table 3.
Three, moisture-keeping efficacy evaluation
1. experimental principle
Human experimentation, by particular experiment, crowd forms test population, and test subject makes the variation of (and cosmetic industry composition) front and back moisture of skin and moisture loss that applies some make up, thereby determines the moisture-keeping efficacy of makeup (or functional component).
2. laboratory apparatus and sample
Laboratory apparatus: moisture content of skin testing tool Corneometer CM825, moisture are through the lost testing tool Tewameter TM300 of skin.
Sample: Sensor Chip CM 5 astringent, containing Sensor Chip CM 5 0.1%.
3. experiment crowd and the test duration
Experimenter amounts to 30 people.Concrete sex composition and age composition be defined as at random 18-25,25-35,35-50 year each five of each age group men and women.
4. experimental technique
(1) select the left and right arm of experimenter cycle labeling successively: tested region samples group and blank region;
(2) technician uses moisture content of skin testing tool Corneometer CM825, moisture to measure through the lost testing tool Tewameter TM300 of skin, averages, and is designated as blank value.
(3) smear sample at experimenter's cheek, use successively: Sensor Chip CM 5 (prescription).
(4) experimenter makes to apply some make up after one hour, two hours, four hours continuously, uses moisture content of skin testing tool Corneometer CM825, moisture to scatter and disappear and test Tewameter TM300 measurement 5 times through skin by technician, averages.
(5) numerical value that statistics records, analyzes its moisture of skin and moisture loss Changing Pattern.
5. interpretation of result
(1) moisture content change
Moisture content change was reflected in test period, Experimental Area moisture content change with time.Its value is larger, and moisture content is larger, otherwise moisture content is less.
Make the curve of moisture content change with time for reference examples and each embodiment, can find out that embodiment 1-6 has all embodied good moisture retention, there were significant differences (p<0.05) with blank group.For example, the moisture content change with time of embodiment 5 as shown in Figure 2.
(2) moisture loss situation
Moisture loss variation was reflected in test period, Experimental Area moisture loss change with time.Its value is less, and moisture loss is fewer, and lock outlet capacity is stronger; Otherwise lock outlet capacity is more weak.
Make the curve of water loss amount change with time for reference examples and each embodiment, can find out that embodiment 1-6 has all embodied good moisture retention, each time period sample sets is compared and is all had significant difference (p<0.05) with blank group water loss amount, and always lower than blank group, the water loss amount change with time of for example embodiment 5 as shown in Figure 3.
Four, senile-resistant efficacy (hydroxy radical qiao is removed experiment)
1. laboratory apparatus and reagent
Thermostat water bath, whizzer, spectrophotometer, colorimetric cylinder
H
2o
2, FeSO
4, Whitfield's ointment, deionized water
2. experimental technique
(1) in colorimetric cylinder, add a certain amount of 2mmol/L FeSO
4the each 3mL of solution, 1mmol/LH
2o
2the each 3mL of solution, shakes up;
(2) add 6mmol/L Whitfield's ointment 3mL, shake up, after heating in water bath, take out, under 510nm, survey its absorbance A
0;
(3) then add respectively 1% liquid 1.0ml to be measured, shake up, continue heating in water bath, take out and survey its absorbance A x;
(4) reduction of the system absorbance causing for the common 1.0ml liquid to be measured that adds after eliminating and distilled water, method is the same, surveys its absorbance A after water bath with thermostatic control
00, add 1ml distilled water, after shaking up, survey again once its absorbance A xx, A
reduce=A
00-Axx.
(5) liquid to be measured to OH clearance rate is: OH clearance rate (%)=100 (A
0-Ax-A
reduce)/A
0calculate the hydroxyl radical free radical clearance rate of reference examples and each embodiment, visible embodiment 1-6 has embodied certain anti-ageing effect, and concrete data are in table 3.
Each parametric measurement result of embodiment and prior art is as shown in table 3 below.
Interpretation of result
By relatively drawing above, the indices of carboxymethyl yeast glucan in various embodiments of the present invention, substitution value, dissolving power, moisture-keeping efficacy, skin regeneration speed all reach requirement, substitution value all reaches 0.3-1.0,2% solution is clear solution, molecular weight all reaches 100-1000KD, all has moisture-keeping efficacy, and Scavenging action to hydroxyl free radical all reaches more than 60%.
And wherein, preferred embodiment is 3,4, more preferably 5,6, most preferred embodiment is that the substitution value of 5, embodiment 5 reaches 0.75, and molecular weight reaches 530KD, and Scavenging action to hydroxyl free radical reaches 89%.
By the contrast of embodiment 1 and reference examples 1, the add-on of etoh solvent when known alkalinization step, has affected the effect of preparing to a great extent, it is the product substitution value 0.1 3 that reference examples 1 obtains, 2% solution is opaque, and molecular weight 780KD, does not have moisture-keeping efficacy, Scavenging action to hydroxyl free radical only has 18%, the product substitution value that visible reference examples 1 obtains is extremely low, level of response deficiency, and therefore molecular weight is very high, water-soluble hardly, substantially do not reach anti-ageing moisturizing texts.
By the contrast of embodiment 1-6 and reference examples 2, known existing what slurry method, although by adjusting alkalizing agent, the amount of etherifying reagent, strengthens level of response, can not obtain as the result of embodiments of the invention 1-6, for example the Scavenging action to hydroxyl free radical of reference examples 2 only has 42%, and the amount of reagent used due to reference examples 2 substantially exceeded various embodiments of the present invention, therefore not only the impact of performance is better in the present invention, and has saved the amount of the reagent such as ethanol, alkali.
Finally reach a conclusion, method of the present invention alkalizes and concentration and the condition of etherification reaction by adjustment, and reactivity is improved, the molecular weight product obtaining changes little, antioxidant property is better, and has greatly saved the reaction cost such as reagent cost, has very significantly advantage.
Claims (13)
1. a preparation method for carboxymethyl yeast glucan product, is characterized in that, comprises the following steps:
A alkalization: add alkali according to every 1kg yeast glucan containing the ratio of hydroxide radical 12.5～37.5mol in yeast glucan, be that 1:0.5～2.5 add ethanol according to yeast glucan and ethanol mass ratio, in 0～30 ℃ of reaction simultaneously;
B etherificate: in the solution obtaining in steps A, be that 1:0.3～1.0 add Mono Chloro Acetic Acid by yeast glucan and chloroacetic mass ratio, be that 1:0.5～1.5 add ethanol by yeast glucan and ethanol mass ratio simultaneously, in 30～80 ℃ of reactions, cooling;
C washing: in the solution obtaining at step B, add washing with alcohol, with hydrochloric acid neutralization, washing, separates and be precipitated carboxymethyl yeast glucan simultaneously;
Preferably, also comprise following filtration step or drying step:
D filters: the Sensor Chip CM 5 that step C is obtained is dissolved in water, adopts diatomite filtration to obtain carboxymethyl yeast glucan liquid product, and while preferably making Sensor Chip CM 5 concentration be 1%-4%, its 600nm OD value is less than 0.4;
E is dry: the carboxymethyl yeast glucan that step C is obtained dry, pulverize and obtains carboxymethyl yeast glucan powder-like product, be preferably dried to moisture to be less than≤8%.
2. a preparation method as claimed in claim 1, is characterized in that, the yeast glucan of described steps A is 1:0.7～1.0, most preferably 1:0.7 with the mass ratio of the alkali adding.
3. a preparation method as claimed in claim 1 or 2, is characterized in that, the yeast glucan of described steps A is 1:1.0～2.0, most preferably 1:1.0 with the mass ratio of the ethanol adding.
4. the preparation method as described in claim 1～3 any one, is characterized in that, the yeast glucan of described step B and chloroacetic mass ratio are 1:0.5～0.8, most preferably 1:0.5.
5. the preparation method as described in claim 1～4 any one, is characterized in that, the yeast glucan of described step B is 1:0.5～1.0, most preferably 1:0.5 with the mass ratio of the ethanol adding.
6. the preparation method as described in claim 1～5 any one, is characterized in that, the yeast glucan of described step C is 1:3～1 0 with adding the mass ratio of ethanol; Preferably 1:8～1 0, most preferably 1:8.
7. the preparation method as described in claim 1～6 any one, is characterized in that, each step alcohol concn used is 70～95%.
8. the preparation method as described in claim 1～7 any one, is characterized in that, the alkali of described steps A is NaOH or KOH, preferably pure NaOH or KOH.
9. the preparation method as described in claim 1～8 any one, is characterized in that, the temperature of reaction of described steps A is 0～20 ℃, preferably 0-10 ℃, most preferably 0 ℃.
10. the preparation method as described in claim 1～9 any one, is characterized in that, the temperature of reaction of described step B is 40～70 ℃, preferably 50-60 ℃, most preferably 50 ℃.
11. preparation methods as described in claim 1～1 0 any one, is characterized in that, described steps A quaternization and step B etherification reaction all carry out in kneader.
Carboxymethyl yeast glucan product prepared by 12. methods as described in claim 1～11 any one, is characterized in that, substitution value 0.3～1.0, and preferably 0.5～0.75, molecular weight 1 00-1 000KD, is preferably 200-500KD, in water, dissolves completely.
13. 1 kinds of carboxymethyl yeast glucan products, is characterized in that, substitution value 0.3～1.0, and preferably 0.5～0.75, molecular weight 100-1000KD, is preferably 200-500KD, in water, dissolves completely.
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| CN109528525A (en) * | 2018-12-29 | 2019-03-29 | 樊利平 | A kind of anti-aging eye cream and preparation method thereof |
| CN117487037A (en) * | 2023-12-29 | 2024-02-02 | 山东国力生物科技有限公司 | Method for preparing multiposition substituted carboxymethyl dextran and its product |
| CN119161601A (en) * | 2024-11-19 | 2024-12-20 | 齐鲁工业大学(山东省科学院) | A multi-component composite hydrogel, preparation method and application thereof |
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| CN108611385A (en) * | 2016-12-12 | 2018-10-02 | 安琪酵母股份有限公司 | Water-soluble yeast beta-dextran and its preparation method and application |
| CN108245468A (en) * | 2018-03-30 | 2018-07-06 | 广州科盈化妆品有限公司 | Hormone face repair essence containing traditional Chinese medicinal ingredients and preparation method thereof |
| CN109010245A (en) * | 2018-09-13 | 2018-12-18 | 广东丸美生物技术股份有限公司 | Anti-acne preparations |
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