CN103750846A - Cobitidae fish blood fixation method - Google Patents
Cobitidae fish blood fixation method Download PDFInfo
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- CN103750846A CN103750846A CN201410036989.1A CN201410036989A CN103750846A CN 103750846 A CN103750846 A CN 103750846A CN 201410036989 A CN201410036989 A CN 201410036989A CN 103750846 A CN103750846 A CN 103750846A
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- blood
- syringe
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- fixative
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- 239000008280 blood Substances 0.000 title claims abstract description 35
- 210000004369 blood Anatomy 0.000 title claims abstract description 35
- 241000252185 Cobitidae Species 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title abstract description 5
- 241000251468 Actinopterygii Species 0.000 claims abstract description 20
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 10
- 239000001509 sodium citrate Substances 0.000 claims abstract description 10
- 239000000834 fixative Substances 0.000 claims abstract 5
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims abstract 2
- 229960002897 heparin Drugs 0.000 claims abstract 2
- 229920000669 heparin Polymers 0.000 claims abstract 2
- 241000685317 Triplophysa Species 0.000 claims description 10
- 210000003462 vein Anatomy 0.000 claims description 3
- 238000010241 blood sampling Methods 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract 1
- 230000001112 coagulating effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 1
- 241001275867 Schizothorax Species 0.000 description 1
- 210000004712 air sac Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000002489 hematologic effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000004894 snout Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明涉及一种鳅科鱼类血液固定方法，将用于固定鳅科鱼类血液的固定液(柠檬酸钠或肝素)先放入注射器中，然后用注射器直接从鳅科鱼类机体上(通常在尾部)采集血样，由于注射器中有预先放入的固定液(柠檬酸钠)，所以从鳅科鱼类机体上采集的血液进入注射器后直接被固定，再送到实验室冷藏保存，以备后用。这样就避免血液样本的浪费，使得血液固定简单化，所以本方法简单易学，适用于鳅科鱼类机体血液固定。The invention relates to a method for fixing the blood of Loach family fish. The fixative (sodium citrate or heparin) used for fixing the blood of Loach family fish is first put into a syringe, and then the syringe is used to directly extract the blood from the body of Loach family fish ( Blood samples are usually collected from the tail), and since the syringe has a pre-placed fixative (sodium citrate), the blood collected from the loach family fish body will be fixed directly after entering the syringe, and then sent to the laboratory for refrigerated storage for future use. Use later. In this way, the waste of blood samples is avoided, and the blood fixation is simplified. Therefore, the method is simple and easy to learn, and is suitable for blood fixation of fishes of the family Loachidae.
Description
技术领域technical field
本发明涉及一种鳅科鱼类血液固定方法。The invention relates to a method for fixing blood of loach family fish.
背景技术Background technique
叶尔羌高原鳅Triplophysa(Hedinichthys)yarkandensis(Day)，地方名为狗头鱼，隶属鲤形目、鳅科、条鳅亚科、高原鳅属、鼓鳔亚属；广泛分布于塔里木河水系，塔里木河特有鱼类，分布海拔最高的鱼类之一。近年来，由于塔里木河水系水体的污染，环境破坏，叶尔羌高原鳅资源锐减，可能会成为继扁吻鱼和塔里木裂腹鱼之后的第三种塔里木河的濒危鱼类，需要亟待保护。Yarkand plateau loach Triplophysa (Hedinichthys) yarkandensis (Day), the local name doghead fish, belongs to Cyprinidae, Loachidae, Loach subfamily, Plateau loach genus, Swimbladder subgenus; widely distributed in the Tarim River system, Tarim River endemic fish, one of the highest distribution of fish. In recent years, due to the pollution of the Tarim River water system and the destruction of the environment, the resources of the Yarkand plateau loach have declined sharply. It may become the third endangered fish species in the Tarim River after the flat snout and the Tarim Schizothorax, and it needs to be protected urgently. .
近年来，对于鱼类生理生化的研究较多，尤其是对于这些易危鱼类或濒危鱼类生活环境的调查，环境胁迫的研究等，都必须进行血液学的研究。对于个体较大的鱼类而言，采集血液比较容易，但对于个体较小，血液要求较高的鱼类而言，是比较困难的，再加上固定，更是很难完成的事情。In recent years, there have been many studies on the physiology and biochemistry of fish, especially for the investigation of the living environment of these vulnerable fish or endangered fish, and the study of environmental stress, etc., all must carry out hematological research. For larger fish, it is easier to collect blood, but for smaller fish with higher blood requirements, it is more difficult, and it is even more difficult to complete when it is fixed.
发明内容Contents of the invention
鉴于叶尔羌高原鳅为无鳞鱼类，个体较小，资源锐减的鱼类之一，为了更好的开展其生理生化学等方面的研究，特此进行了血液采集，以便进行相关研究。In view of the fact that the Yarkand plateau loach is a scaleless fish with small individuals and one of the fishes whose resources are sharply reduced, in order to better carry out research on its physiology and biochemistry, blood collection is hereby carried out for related research.
将采集的叶尔羌高原鳅先行养殖者水族箱中，待采集血液。Put the collected Yarkand plateau loach in the aquarium of the breeder first, and wait for the blood to be collected.
将配置好柠檬酸钠分成2份以上，一份中放置离心管待加血液样品，一份待放到注射器中。Divide the prepared sodium citrate into two or more parts, place a centrifuge tube in one to add blood samples, and put the other in a syringe.
将叶尔羌高原鳅麻醉保定或不麻醉保定，用干净毛巾擦拭鱼体，尤其是尾柄采血处，要保持干净，采用1.0ml或2.0ml的注射器，并在注射器中先行抽取少量柠檬酸钠。Anesthetize or not anesthetize the Yeerqiang plateau loach, wipe the fish body with a clean towel, especially the tail peduncle blood collection place, keep it clean, use a 1.0ml or 2.0ml syringe, and first extract a small amount of sodium citrate in the syringe .
在叶尔羌高原鳅尾柄尾静脉采血，用抽取少量柠檬酸钠注射器采血，当血液进入注射器后随即被固定，以免抽取时间过长凝固。Blood was collected from the caudal vein of the loach on the Yarkand plateau, and a small amount of sodium citrate was used to collect blood with a syringe. When the blood entered the syringe, it was fixed immediately to prevent it from coagulating for too long.
具体实施方式Detailed ways
在下文中，现在将更充分地描述本发明，示出了各种实施例。然而，本发明可以以许多不同的形式来实施，且不应该解释为局限于在此阐述的实施例。相反，提供这些实施例使得本公开将是彻底和完全的，并将本发明的范围充分地传达给本领域技术人员。In the following text, the invention will now be described more fully, illustrating various embodiments. However, this invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
将采集的叶尔羌高原鳅先行养殖于水族箱中，待采集血液。The collected Yarkand plateau loach was cultured in the aquarium first, and the blood was collected.
将配置好柠檬酸钠分成2份以上，一份中放置离心管待加血液样品，一份待放到注射器中。Divide the prepared sodium citrate into two or more parts, place a centrifuge tube in one to add blood samples, and put the other in a syringe.
将叶尔羌高原鳅麻醉保定或不麻醉保定，用干净毛巾擦拭鱼体，尤其是尾柄采血处，要保持干净，采用1.0ml或2.0ml的注射器，并在注射器中先行抽取少量柠檬酸钠。Anesthetize or not anesthetize the Yeerqiang plateau loach, wipe the fish body with a clean towel, especially the tail peduncle blood collection place, keep it clean, use a 1.0ml or 2.0ml syringe, and first extract a small amount of sodium citrate in the syringe .
在叶尔羌高原鳅尾柄尾静脉采血，用抽取少量柠檬酸钠注射器采血，当血液进入注射器后随即被固定，以免抽取时间过长凝固。Blood was collected from the caudal vein of the loach on the Yarkand plateau, and a small amount of sodium citrate was used to collect blood with a syringe. When the blood entered the syringe, it was fixed immediately to prevent it from coagulating for too long.
以上所述仅为本发明的实施例而已，并不用于限制本发明。本发明可以有各种合适的更改和变化。凡在本发明的精神和原则之内所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。The above descriptions are only examples of the present invention, and are not intended to limit the present invention. Various suitable modifications and variations are possible in the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.
Claims (4)
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| CN201410036989.1A CN103750846A (en) | 2014-01-24 | 2014-01-24 | Cobitidae fish blood fixation method |
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| CN201410036989.1A CN103750846A (en) | 2014-01-24 | 2014-01-24 | Cobitidae fish blood fixation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112167121A (en) * | 2020-10-16 | 2021-01-05 | 国家能源集团新疆开都河流域水电开发有限公司 | Method for cultivating long-body plateau loach fry |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998025523A1 (en) * | 1996-11-25 | 1998-06-18 | Symbiosis Corporation | Biopsy forceps instrument having irrigation and aspiration capabilities |
| JP2004290517A (en) * | 2003-03-27 | 2004-10-21 | Olympus Corp | Biopsy needle |
| CN101035459A (en) * | 2004-10-05 | 2007-09-12 | 奥林巴斯株式会社 | Endoscope system, biopsy-sample container, method of obtaining biopsy samples, and method of processing biopsy samples |
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2014
- 2014-01-24 CN CN201410036989.1A patent/CN103750846A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998025523A1 (en) * | 1996-11-25 | 1998-06-18 | Symbiosis Corporation | Biopsy forceps instrument having irrigation and aspiration capabilities |
| JP2004290517A (en) * | 2003-03-27 | 2004-10-21 | Olympus Corp | Biopsy needle |
| CN101035459A (en) * | 2004-10-05 | 2007-09-12 | 奥林巴斯株式会社 | Endoscope system, biopsy-sample container, method of obtaining biopsy samples, and method of processing biopsy samples |
Non-Patent Citations (2)
| Title |
|---|
| 傅晓龙: "《大白鼠大剂量静脉血心脏快速采血方法的改进》", 《中国组织工程研究与临床康复》, vol. 13, no. 7, 12 February 2009 (2009-02-12), pages 1279 - 1282 * |
| 高建军: "《泥鳅的血液学及湖北省泥鳅群体的遗传多样性研究》", 《华中农业大学硕士学位论文》, 31 December 2010 (2010-12-31), pages 13 - 15 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112167121A (en) * | 2020-10-16 | 2021-01-05 | 国家能源集团新疆开都河流域水电开发有限公司 | Method for cultivating long-body plateau loach fry |
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Application publication date: 20140430 |