CN103739059B - A kind of carrier attached type Cyclic culture device of heterotrophic nitrification-aerobic denitrification microbial inoculum - Google Patents

Abstract

The invention discloses a carrier-attached circulation culture device for heterotrophic nitrification-aerobic denitrification bacteria agent, which comprises a main reactor and a pre-reactor; the top of each reactor is provided with a feeding port and a medium injection port, The lower part is provided with an aeration device, a settling area and a discharge valve, which are connected to two solid-liquid separators through the discharge valve; the main reactor is connected to the solid-liquid separator of the pre-reactor through a feeding port. The pre-reactor uses the residual liquid after the main reactor for re-cultivation, and the unsaturated carrier is transferred to the main reactor for further cultivation. The present invention not only makes full use of the culture medium, but also provides a pre-attachment process for the carrier, improving the The utilization rate of the medium and the attachment efficiency of the carrier avoid excessive loss of the bacterial agent, improve the stability and tolerance of the bacterial agent, and realize the self-controlled continuous operation of the two reactors.

Description

A carrier-attached circulation culture device for heterotrophic nitrification-aerobic denitrification bacteria agent

technical field

The invention belongs to environmental engineering water treatment microbial bacterial agent cultivation, in particular to a carrier-attached circulation culture device for heterotrophic nitrification-aerobic denitrification bacterial agent.

Background technique

Circulation culture is a culture method in which cells are retained in a culture tank in some way for recycling, usually by sedimentation, centrifugation and membrane filtration. Each of these methods has some advantages and disadvantages. The sedimentation method has simple equipment and low energy consumption. However, due to the dense medium, especially in the later stage of cultivation, the organism and the medium are mixed together in a jelly-like shape, which is difficult to settle and the culture waste is difficult to separate. ;The centrifugation method is suitable for industrial culture media containing various particles, and is more suitable for large-scale systems, but the process is more complicated and the energy consumption is higher; the membrane filtration method is more thorough in removing waste liquid, but the membrane is easily blocked, and pumps need to be added. equipment, high energy consumption. These cyclic culture methods not only have some defects in the cultivation stage, but also have some problems in the preservation stage of the bacterial agent. Because the moisture content of the bacterial agent is high, and it is not easy to use methods such as air drying to remove water, the dry storage of the bacterial agent is more difficult.

The carrier attachment method is a high-density culture method. After adding the carrier to the medium, most organisms will attach to the inner and outer surfaces of the carrier and grow, which can solve the problem of dispersed growth of organisms and high solid moisture content in the traditional microbial culture method. As for the problem of difficult solid-liquid separation, the waste liquid can be separated efficiently by adopting a suitable method, which can improve the shortcomings of sedimentation, centrifugation and membrane filtration in the separation of waste liquid. In terms of bacterial agent preservation, carrier-attached cyclic culture can also overcome the defects of traditional cyclic culture. Because the bacterial agent is attached to the carrier, the organism is supported, and the carrier has a high porosity, making air-dried storage convenient and fast.

Contents of the invention

The purpose of the present invention is to address the shortcomings of traditional bacterial agent circulation culture methods, such as sedimentation, centrifugation and membrane filtration, etc. in waste liquid separation and dry storage, and to combine carrier attachment with cycle culture to provide a method to achieve efficient culture of bacterial agents new culture device.

The present invention is realized through the following technical solutions.

A carrier-attached circulation culture device for heterotrophic nitrification-aerobic denitrification bacteria agent, comprising a reactor, a liquid storage tank, a centrifugal pump and a circulation pipeline, characterized in that the reactor is the main reactor 1 and pre-reactor 16;

The inside of the main reactor 1 is provided with an outer cylinder A2 and an inner cylinder A3, the top of which is provided with a feeding port A11 and a medium injection port A12, and the lower part is provided with an aeration device A5 and a sedimentation area A6. The bottom of A6 is provided with a discharge valve A7; the main reactor is connected to the solid-liquid separator A8 through the discharge valve A7; the lower side of the solid-liquid separator A8

A grille A29 and a residual liquid discharge port A30 are provided. A solid-liquid separator valve A9 is provided at the bottom of the solid-liquid separator A8. The bottom of the solid-liquid separator valve A9 is in the shape of a conical funnel. Saturated carrier discharge port A10; the middle part of the outer surface of the main reactor 1 is also provided with a liquid level gauge A4;

The inside of the prereactor 16 is provided with an outer cylinder B17 and an inner cylinder B18, its top is provided with a feed port B25 and a medium injection port B26, its lower part is provided with an aeration device B20, and a settling area B21 is provided. In the settling area The bottom of B21 is provided with a discharge valve B22; the pre-reactor is connected with the solid-liquid separator B23 through the discharge valve B22; the lower side of the solid-liquid separator B23 is provided with a grid B27 and a waste liquid discharge port B28, solid-liquid The bottom of the separator B23 is provided with a solid-liquid separator valve B24, the bottom of the solid-liquid separator valve B24 is in the shape of a conical funnel, and the end of the conical funnel is connected to the feeding port A11 at the top of the main reactor 1; the pre-reactor 16 The middle part of the outer surface is also provided with a liquid level gauge B19;

The raffinate discharge port A30 at the bottom of the solid-liquid separator A8 of the main reactor 1 is connected to the liquid storage tank 15, and the culture medium in the liquid storage tank 15 communicates with the top of the pre-reactor 16 through the centrifugal pump 14 and the culture medium circulation pipe 13 , so that the culture medium in the two reactors can realize internal circulation through the action of the centrifugal pump 14 .

Both the main reactor 1 and the prereactor 16 are airlift reactors.

The effective volumes of the main reactor 1 and the pre-reactor 16 are respectively 0.8 m ³ .

The diameters of the inner cylinder A3 and the inner cylinder B18 of the main reactor 1 and the prereactor 16 are 0.5m,

The diameters of the outer cylinder A2 and the outer cylinder B17 are both 1 m, and the heights of the two reactors are both 2 m.

The grid spacing of the grid A29 and the grid B27 is 2 cm.

Compared with the traditional sedimentation, centrifugation and membrane filtration circulation culture device, the present invention has most of the bacterial agents attached to the carrier, and the waste liquid can be easily separated by the grid, avoiding excessive loss of the bacterial agents, and air-dried storage is more convenient It is fast, and can also protect microbial cells from the influence of water shear force, obtain a higher cell concentration, and improve the stability and tolerance of the bacterial agent. In addition, the present invention continues to utilize the culture medium waste liquid of the main reactor in the pre-reactor, which not only makes full use of the culture medium, but also provides a pre-attachment process for the carrier, which improves the efficiency of the carrier attachment. Two reactors constitute The circulation of carrier and culture medium is realized, and automatic control and continuous operation are realized.

Description of drawings

Fig. 1 is a schematic structural view of a carrier-attached circulation culture device of the present invention.

The reference signs in Fig. 1 are as follows:

1——Main Reactor 2——Outer Cylinder A

3——Inner cylinder A 4——Level gauge A

5——Aeration device A 6——Sedimentation area A

7——discharging valve A 8——solid-liquid separator A

9——solid-liquid separator valve A 10——saturated carrier discharge port A

11——feeding port A 12——medium injection port A

13——Medium circulation pipe 14——Centrifugal pump

15——reservoir 16——prereactor

17——Outer cylinder B 18——Inner cylinder B

19——Level gauge B 20——Aeration device B

21——Sedimentation area B 22——Discharge valve B

23——solid-liquid separator B 24——solid-liquid separator valve B

25——feeding port B 26——medium injection port B

27——Grille B 28——Waste liquid discharge port B

29——Grille A 30——Residual liquid discharge port A

Detailed ways

A carrier-attached circulation culture device for heterotrophic nitrification-aerobic denitrification bacterial agent of the present invention, the main reactor and the pre-reactor are made of materials such as stainless steel and plexiglass, and are prepared by a conventional production process. The two reactors are both airlift reactors, and the effective volumes of the two reactors are 0.8m ³ respectively. The diameters of the inner cylinder A3 and the inner cylinder B18 of the main reactor 1 and the prereactor 16 are both 0.5m, and the outer cylinder The diameters of A2 and outer cylinder B17 are both 1 m, and the heights of the two reactors are both 2 m. In actual production, the specific size can also be determined according to actual needs.

The inside of the main reactor 1 is provided with an outer cylinder A2 and an inner cylinder A3, the top of which is provided with a feeding port A11 and a medium injection port A12, and the lower part is provided with an aeration device A5 and a sedimentation area A6. The bottom of A6 is provided with a discharge valve A7; the main reactor is connected with the solid-liquid separator A8 through the discharge valve A7; the lower side of the solid-liquid separator A8 is provided with a grid A29 and a raffinate discharge port A30, solid-liquid The bottom of the separator A8 is provided with a solid-liquid separator valve A9, and the bottom of the solid-liquid separator valve A9 is in the shape of a conical funnel, and the end of the conical funnel is provided with a saturated carrier discharge port A10; the middle of the outer surface of the main reactor 1 The part is also equipped with a liquid level gauge A4;

The inside of the prereactor 16 is provided with an outer cylinder B17 and an inner cylinder B18, its top is provided with a feed port B25 and a medium injection port B26, its lower part is provided with an aeration device B20, and a settling area B21 is provided. In the settling area The bottom of B21 is provided with a discharge valve B22; the pre-reactor is connected with the solid-liquid separator B23 through the discharge valve B22; the lower side of the solid-liquid separator B23 is provided with a grid B27 and a waste liquid discharge port B28, solid-liquid The bottom of the separator B23 is provided with a solid-liquid separator valve B24, the bottom of the solid-liquid separator valve B24 is in the shape of a conical funnel, and the end of the conical funnel is connected to the feeding port A11 on the top of the main reactor 1; the pre-reactor 16 The middle part of the outer surface is also provided with a liquid level gauge B19;

The diameters of the inner cylinder A3 and the inner cylinder B18 of the main reactor 1 and the prereactor 16 are 0.5m, the diameters of the outer cylinder A2 and the outer cylinder B17 are 1m, and the heights of the two reactors are 2m. The grid spacing between the grid A29 and the grid B27 is 2 cm.

The raffinate discharge port A30 at the bottom of the solid-liquid separator A8 of the main reactor 1 is connected to the liquid storage tank 15, and the culture medium in the liquid storage tank 15 communicates with the top of the pre-reactor 16 through the centrifugal pump 14 and the culture medium circulation pipe 13 , so that the culture medium in the two reactors can realize internal circulation through the action of the centrifugal pump 14 .

Adopt culture device of the present invention to carry out the culture process of heterotrophic nitrification-aerobic denitrification bacterial agent, concrete steps are as follows:

(1) start

①In order to form a gradient of carrier attachment in the reactor, add 0.4m ³ packing in four times. In the main reactor 1 and the pre-reactor 16, add respectively total filler volume 1/4 namely 0.1m The spherical plastic carrier of 0.1m ³ , and fill the two reactors with the inoculated heterotrophic nitrification-aerobic denitrification bacterial agent For culture medium, turn on the aeration device A5 and aeration device B20, adjust the aeration rate to 0.3-0.6m ³ /h to ensure good fluidization effect in the two reactors, and start culturing;

The main reactor 1 and the prereactor 16 are airlift reactors.

The total packing volume is 1/2 of the effective volume of the main reactor 1 or the pre-reactor 16; the effective volumes of the main reactor 1 and the pre-reactor 16 are both 0.8m ³ .

The spherical plastic filler as the carrier is a lightweight filler with a diameter of 3 cm.

The culture medium is beer wastewater, which can also include papermaking wastewater, food factory wastewater, leather wastewater and various industrial processing wastewater;

② The first cycle is 0-24h. Since the filler attachment time is short, the filler has not reached saturation, and the settled filler is less, so the circulation of the culture medium in the main reactor 1 is carried out first;

Open the discharge valve A7 of the main reactor 1, the culture medium may entrain a little filler, which is neglected for now and enter the solid-liquid separator A8, and the residual liquid of the culture medium flows out through the grid A29 and the residual liquid discharge port A30, and enters the liquid storage tank 15 reserve, when the liquid level in the main reactor 1 drops to the liquid level height set by the liquid level meter A4, that is, the 1/2 position of the initial liquid level, the automatic control device automatically closes the discharge valve A7, and at the same time through the medium injection port A12 Supplement fresh culture medium to the main reactor 1 to the original liquid level, and add 1/4 of the total filler volume, that is, 0.1m ³ fresh spherical Plastic carrier; adjust the aeration rate of the two reactors to 0.3-0.6m ³ /h to ensure a good fluidization effect;

③ In the second cycle, namely 24-48 hours, the settled filler is still less, and the filler has not yet reached saturation, so the culture medium circulation of the pre-reactor 16 and the main reactor 1 is carried out at the same time;

The automatic control device opens the discharge valve A7 and discharge valve B22 of the two reactors at the same time. The culture medium may contain a little filler, which is ignored for the time being. Enter the solid-liquid separator A8 and solid-liquid separator B23; enter the main reactor 1 solid-liquid The culture medium residual liquid of separator A8 flows out through grid A29 and residual liquid discharge port A30, and enters liquid storage tank 15 for storage; the culture medium waste liquid entering pre-reactor 16 solid-liquid separator B23 passes through grid B27, waste liquid The liquid discharge port B28 flows out and is directly discharged into the waste liquid pool outside the system; when the liquid level in the two reactors drops to the liquid level height set by the liquid level gauge A4 and the liquid level gauge B19, which is 1/2 of the initial liquid level position, the automatic control device automatically closes the discharge valve A7 and the discharge valve B22, and at the same time replenishes fresh culture medium to the main reactor to the original liquid level; the automatic control device starts the centrifugal pump 14, and the culture medium in the reservoir A15 The raffinate is pumped into the pre-reactor 16 to reach the original liquid level, and then respectively add 1/4 of the total filler volume respectively in the two reactors, that is, 0.1 ^m Fresh spherical plastic carrier; adjust the exposure of the two reactors The air volume is 0.3～0.6m ³ /h to ensure good fluidization effect;

④The third cycle is 48~72h, repeat step 3;

⑤ In the fourth cycle, which is 72-96 hours, the growth of the first batch of spherical plastic carrier bacterial agents of 1/4 of the total filler volume of each of the two reactors, which is 0.1m ³ , reaches the maximum, enters a settleable state, and deposits on the sedimentation In the area A6 and the precipitation area B21; the filler in the main reactor 1 is cultivated due to the supplement of fresh culture medium, and the filler is basically saturated; the filler in the pre-reactor 16 is not saturated due to the circulation of the culture medium residue. Further attachment culture is required, so the filler and the culture medium are circulated; the automatic control device opens the discharge valves of the two reactors at the same time, and the culture medium entrains the filler into the solid-liquid separator A8 and the solid-liquid separator B23; into the main reactor 1 The culture medium raffinate of the solid-liquid separator A8 flows out through the grid A29 and the raffinate discharge port A30, and enters the liquid storage tank 15 for storage; the waste liquid of the culture medium entering the pre-reactor 16 solid-liquid separator B23 passes through the grid The grid B27 and the waste liquid discharge port B28 flow out, and are directly discharged into the waste liquid pool outside the system. The fillers in the two solid-liquid separators are intercepted by the grid A29 and the grid B27 and remain in the solid-liquid separator A8 and solid-liquid separation. In the reactor B23; when the liquid level in the two reactors drops to the liquid level height set by the liquid level gauge A4 and the liquid level gauge B19, that is, the 1/2 position of the initial liquid level, the automatic control device automatically closes the discharge valve A7 and the discharge valve. Feed valve B22; after 30s, when the liquid flows out, the automatic control device opens the solid-liquid separator valve A9 and the solid-liquid separator valve B24 at the bottom of the solid-liquid separator of the two reactors, and the pre-reactor 16 solid-liquid separator B23 The carrier flows into the main reactor 1 by gravity; the carrier in the solid-liquid separator A8 of the main reactor 1 is discharged from the system through the saturated carrier discharge port A10, and enters the air-drying area. After the carrier has been cleaned and sterilized, it can be used as a supplementary carrier for the pre-reactor 16 again; then the main reactor 1 is replenished with fresh culture medium to the original level; The residual liquid of the base liquid is pumped into the pre-reactor 16 to reach the original liquid level; 1/4 of the total packing volume, that is, ^0.1m3 new spherical plastic carrier is added to the pre-reactor to adjust the aeration rate of the two reactors 0.3～0.6m ³ /h to ensure a good fluidization effect; at this time, the start-up is completed;

(2) run

Every other cycle, that is, 24 hours, in the two reactors, there will be 1/4 of the total filler volume, that is, 0.1m ³ new spherical plastic carriers, which will be deposited in the precipitation area A6 and the precipitation area B21 due to gravity greater than the water flow uplift force, the main reaction The carrier in the precipitation zone A6 of the prereactor is the saturated carrier that has been attached, and the carrier in the prereactor precipitation zone B21 is the unsaturated carrier that needs to be further attached and cultivated; the automatic control device simultaneously opens the discharge valve A7 and the discharge valve of the main reactor and the prereactor. Feed valve B22, culture medium entrained filler enters solid-liquid separator A8 and solid-liquid separator B23; the residual liquid of culture medium entering main reactor 1 solid-liquid separator A8 flows out through grid A29 and enters liquid storage tank 15 Reserve; the culture medium waste liquid that enters the pre-reactor 16 solid-liquid separator B23 flows out through the grid B27, and directly discharges into the waste liquid pool; the filler is all intercepted by the two grids and stays in the two solid-liquid separators; when the two When the liquid level in each reactor drops to the liquid level height set by the two level gauges, that is, the 1/2 position of the initial liquid level, the automatic control device automatically closes the discharge valve; after 30s, the automatic control device opens two The discharge valve at the bottom of the solid-liquid separator of the reactor, the carrier in the solid-liquid separator B23 of the pre-reactor 16 flows into the main reactor 1 by gravity; the saturated carrier in the solid-liquid separator A8 of the main reactor 1 passes through the saturated carrier discharge port The A10 exhaust system enters the air-drying area, and the air-dried bacterial agent is separated from the carrier and stored at room temperature; after the carrier of the exhaust system is cleaned and disinfected, it can be used as a supplementary carrier for the pre-reactor 16; The culture medium liquid reaches the original liquid level; the automatic control device starts the centrifugal pump 14, and the residual liquid of the culture medium liquid in the liquid storage tank 15 is pumped into the pre-reactor to reach the original liquid level; then the total filler is replenished in the pre-reactor 16 A new spherical plastic carrier with a volume of 1/4, namely 0.1m ³ ; adjust the aeration rate of the two reactors to 0.3-0.6m ³ /h to ensure a good fluidization effect.

The aeration rates of the steps (1) and (2) basically do not need to be adjusted after the operation is stable.

Claims (5)

1. the carrier attached type Cyclic culture device of a heterotrophic nitrification-aerobic denitrification microbial inoculum, comprise reactor, liquid storage tank, impeller pump and circulation line, it is characterized in that, described reactor is main reactor (1) and pre-reactor (16);

The inside of main reactor (1) is provided with urceolus A (2) and inner core A (3), its top is provided with material-feeding port A (11) and substratum inlet A (12), its underpart is provided with aerating apparatus A (5), and be provided with settling region A (6), the bottom of settling region A (6) is provided with discharge valve A (7); Main reactor is connected with solid-liquid separator A (8) by discharge valve A (7); The side, bottom of solid-liquid separator A (8) is provided with grid A (29) and residual liquid discharge mouth A (30), the bottom of solid-liquid separator A (8) is provided with solid-liquid separator valve A (9), in tapered funnel-form below solid-liquid separator valve A (9), the end of tapered funnel is provided with saturated carrier discharge outlet A (10); The middle part of main reactor (1) outside surface is also provided with liquidometer A (4);

The inside of pre-reactor (2) is provided with urceolus B (17) and inner core B (18), its top is provided with material-feeding port B (25) and substratum inlet B (26), its underpart is provided with aerating apparatus B (20), and be provided with settling region B (21), the bottom of settling region B (21) is provided with discharge valve B (22); Pre-reactor is connected with solid-liquid separator B (23) by discharge valve B (22); The side, bottom of solid-liquid separator B (23) is provided with grid B (27) and discard solution discharge port B (28), the bottom of solid-liquid separator B (23) is provided with solid-liquid separator valve B (24), in tapered funnel-form below solid-liquid separator valve B (24), the end of tapered funnel is connected with the material-feeding port A (11) at main reactor (1) top; The middle part of pre-reactor (2) outside surface is also provided with liquidometer B (19);

The residual liquid discharge mouth A (30) of main reactor (1) solid-liquid separator A (8) bottom connects liquid storage tank (15), liquid medium in liquid storage tank (15) is connected with the top of substratum circulation tube (13) with pre-reactor (16) by impeller pump (14), makes the liquid medium in two reactors realize inner circulation by the effect of impeller pump (14).

2. the carrier attached type Cyclic culture device of a kind of heterotrophic nitrification-aerobic denitrification microbial inoculum according to claim 1, its feature can be, described main reactor (1) and pre-reactor (16) are all airlift reactor.

3. the carrier attached type Cyclic culture device of a kind of heterotrophic nitrification-aerobic denitrification microbial inoculum according to claim 1, its feature can be, the useful volume of described main reactor (1) and pre-reactor (16) is respectively 0.8m ³.

4. the carrier attached type Cyclic culture device of a kind of heterotrophic nitrification-aerobic denitrification microbial inoculum according to claim 1, its feature can be, the inner core A (3) of described main reactor (1) and pre-reactor (16), the diameter of inner core B (18) are 0.5m, the diameter of urceolus A (2), urceolus B (17) is 1m, and the height of two reactors is 2m.

5. the carrier attached type Cyclic culture device of a kind of heterotrophic nitrification-aerobic denitrification microbial inoculum according to claim 1, its feature can be, the interval of grid strip of described grid A (29), grid B (27) is 2cm.